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Introduction Type 2 diabetes mellitus (T2DM) is a highly heterogeneous and complex metabolic disease harbouring different metabolic characteristics. Adequate characterisation of subjects is essential to allow the implementation of precision medicine for the prevention, diagnosis, prognosis and treatment of this condition.Methods and analysis This prospective observational cohort study aims to identify and characterise relevant clinical clusters that are reproducibly associated with various clinical outcomes in T2DM in our Mediterranean region. The COPERNICAN study will include 1200 subjects with newly diagnosed T2DM from 28 primary care centres from the city of Barcelona and the healthcare district of Lleida in Catalonia (Spain). Participants will undergo a comprehensive phenotypic evaluation including, among others, six relevant variables: age, antibodies against glutamic acid decarboxylase, body mass index, glycated haemoglobin (HbA1c), indexes of insulin sensibility (HOMA2-IR) and secretion (HOMA2-beta). We will collect additional comprehensive data on glucose-lowering and other drug treatments, clinical evaluation (including complications), laboratory parameters, advanced lipoprotein profile, dietary habits and physical activity. The linkage with the population database will be done to perform a pragmatic follow-up of participants as part of their usual clinical care. A state-of-the-art cluster analysis (k-means and hierarchical clustering) will be performed.Ethics and dissemination The present study complies with all the ethical aspects and protection of participant subjects complying with all current local and European Union legislation. All Ethics Committees from the institutions involved in the study (IR Sant Pau Ethics Committee, Ethics Committee for Drug Research at IDIAP Jordi Gol and University Hospital of Bellvitge Ethics Committee for Research) approved this protocol. Confidentiality and anonymity of the data are ensured according to the current Spanish Organic Law 3/2018 of 05 December.Trial registration number ClinicalTrials.gov. registration number NCT05333718, 27 January 2023.

Cotija cheese is an artisanal matured Mexican cheese from unpasteurized milk. This work determined the microbiological characteristics and behavior of Staphylococcus aureus, Salmonella spp., Listeria monocytogenes and staphylococcal toxin during cheese elaboration and ripening. Sixteen 20-kg cheeses were used, eight inoculated with 6 log CFU/mL of each pathogen, and eight uninoculated, and samples were taken at each stage of the process. In the uninoculated samples, the survival of S. aureus and L. monocytogenes decreased after 30 days of ripening. The average counts of S. aureus in milk, curd, and serum were 7 log MPN /mL, and 8.7 log MPN /g in cheese, decreasing from day 15. Salmonella spp. counts (initial inoculum: 7.2 log MPN /mL) decreased after 24 h, and L. monocytogenes counts (8.7 log MPN/g at 24 h) decreased from day 15 in the cheese. Salmonella spp. and L. monocytogenes were not detected in any sample after 60 days of ripening, unlike S. aureus, which was detected at the end of the study. Lactic acid bacteria counts were 9 log CFU/mL in milk and whey and 7.2 log CFU/g in cheese. Pathogens behavior during the ripening process reduces the health risks by consuming products made with unpasteurized milk when subjected to ripening.

Currently, the high added-value compounds contained in plant by-products and wastes offer a wide spectrum of opportunities for their reuse and valorization, contributing to the circular economy. The bell pepper (Capsicum annum L.) is an exotic vegetable with high nutritional value that, after processing, leaves wastes (peel, seeds, and leaves) that represent desirable raw material for obtaining phytochemical compounds. This review summarizes and discusses the relevant information on the phytochemical profile of bell peppers and their related biological properties as an alternative to revalorize losses and wastes from bell peppers for their application in the food and pharmaceutical industries. Bell pepper fruits, seeds, and leaves contain bioactive compounds (phenols, flavonoids, carotenoids, tocopherol, and pectic polysaccharides) that exhibit antioxidant, antibacterial, antifungal, immunosuppressive and immunostimulant properties, and antidiabetic, antitumoral and neuroprotective activities, and have a potential use as functional food additives. In this context, the revalorization of food waste is positioned as a technological and innovative research area with beneficial effects for the population, the economy, and the environment. Further studies are required to guarantee the safety use of these compounds and to understand their mechanisms of action.

Synbiotic consumption can modulate immune response. This work involves studying the effect of a synbiotic on lymphoid organs and IgA of broilers infected with Salmonella typhimurium and Clostridium perfringens. A total of 258 one-day-old male broilers (Gallus gallus domesticus), line COBBAvian48 (free of growth-promoting antibiotics), were distributed into eight treatment groups. A symbiotic mix comprising Lactobacillus rhamnosus HN001 and Pediococcus acidilactici MA18/5 M as probiotics and 4.5% (0.045 g g−1) of Agave tequilana fructans as prebiotic per dose (one milliliter) was administered through drinking water the first day of life. Bursa, spleen and thymus were analyzed. Broilers treated with the synbiotic, whether or not infected with pathogens, had bigger bursa follicles than the non-treated (p < 0.05), and the ones from the synbiotic group had more lymphocytes than the control group (p < 0.05). Thymus follicles of the synbiotic group were bigger than the control group (p < 0.05). Lesions associated with Salmonella infection were found in the bursa, however, in the broilers treated with the synbiotic, the lesions were less intense and were not present after 32 days of life. The synbiotic mix can stimulate the bursa, increasing the size of their follicles and promoting the ability to resist infections caused by S. typhimurium in broilers.

Synbiotics can prevent gastrointestinal infections in broilers. This work studies the effect of a Synbiotic on broilers. One-day-old male broilers were divided into groups: Control; Synbiotic; Synbiotic + S. Typhimurium; Synbiotic + C. perfringens; Synbiotic + S. Typhimurium + C. perfringens; S. Typhimurium; C. perfringens; and S. Typhimurium + C. perfringens. Histopathological analysis revealed that the Synbiotic promoted longer villi, less deep crypts, and better villi-crypt ratio. Broilers treated with the Synbiotic, infected with pathogens or not, had healthier mucosa. In groups infected with pathogens, the frequency and intensity of histopathologic lesions were lessened often in groups treated with the Synbiotic. The Synbiotic group had higher lactic acid bacteria counts than the Control group on day 39, and the isolation frequency of S. Typhimurium was lower (p < 0.05) in the Synbiotic-treated groups. On day 18, mucosa, villi, villi-crypt ratio, crypt, and feed intake were influenced by Enterobacteriaceae. However, on day 39 (end of the trial), those parameters were influenced by lactic acid bacteria. The Synbiotic influenced morphological modifications in the duodenal mucosa, which in turn gave the broilers the ability to resist infections caused by S. Typhimurium and C. perfringens, by inhibiting their growth and decreasing the intensity and frequency of histopathological injuries.

El presente estudio investigó la correlación entre los niveles de bienestar espiritual y los niveles de ansiedad-estado, en una muestra de 190 pacientes obtenida por medio de muestreo no probabilístico; los criterios de inclusión fueron: adultos de 35 a 85 años de edad, diagnosticados con diabetes mellitus tipo 2, se excluyeron los pacientes con demencia. El marco teórico que guió el estudio es el Modelo de Adaptación de Callista Roy. El diseño fue descriptivo, transversal, correlacional. La muestra poblacional se distribuyó normalmente con una potencia de 80. Se utilizó el cuestionario de espiritualidad de Reed y la escala de ansiedad-estado de Spielberger; los instrumentos presentaron una consistencia interna favorable con un Alpha de Cronbach de .894 y .847 respectivamente. La información se recabó de cuatro instituciones de salud, una del primer nivel y tres del segundo nivel de atención. El análisis de los datos se llevó a cabo a través del paquete estadístico SPSS versión 13. La edad media de los participantes fue de 57,36 años, con una desviación estándar de 11,4; el 55% fueron del sexo femenino, el 83,2% profesa la religión católica, el 51,5% presenta algún tipo de complicación asociada a la diabetes. El coeficiente de correlación entre el bienestar espiritual y la ansiedad-estado fue significativo. Estos resultados apoyan la hipótesis del estudio que refiere a mayor espiritualidad menor nivel de ansiedad-estado, situación que invita a profundizar en el estudio de estos fenómenos tanto en el área educativa como asistencial en la disciplina de enfermería.

El presente estudio investigó la correlación entre los niveles de bienestar espiritual y los niveles de ansiedad-estado, en una muestra de 190 pacientes obtenida por medio de muestreo no probabilístico; los criterios de inclusión fueron: adultos de 35 a 85 años de edad, diagnosticados con diabetes mellitus tipo 2, se excluyeron los pacientes con demencia. El marco teórico que guió el estudio es el Modelo de Adaptación de Callista Roy. El diseño fue descriptivo, transversal, correlacional. La muestra poblacional se distribuyó normalmente con una potencia de 80. Se utilizó el cuestionario de espiritualidad de Reed y la escala de ansiedad-estado de Spielberger; los instrumentos presentaron una consistencia interna favorable con un Alpha de Cronbach de .894 y .847 respectivamente. La información se recabó de cuatro instituciones de salud, una del primer nivel y tres del segundo nivel de atención. El análisis de los datos se llevó a cabo a través del paquete estadístico SPSS versión 13. La edad media de los participantes fue de 57,36 años, con una desviación estándar de 11,4; el 55% fueron del sexo femenino, el 83,2% profesa la religión católica, el 51,5% presenta algún tipo de complicación asociada a la diabetes. El coeficiente de correlación entre el bienestar espiritual y la ansiedad-estado fue significativo. Estos resultados apoyan la hipótesis del estudio que refiere a mayor espiritualidad menor nivel de ansiedad-estado, situación que invita a profundizar en el estudio de estos fenómenos tanto en el área educativa como asistencial en la disciplina de enfermería.