Search

In a plasma containing micrometer-size dust particles, nonlinear wave synchronization was investigated experimentally under microgravity conditions on board the International Space Station (ISS). These dust particles were confined into a 3-D cloud, in the vicinity of a diffuse edge of the plasma, which was generated by an inductively coupled radio frequency (RF) glow discharge. A cross-sectional slab of the cloud was imaged using a video camera. The dust-density fluctuations in the slab were characterized using the video image data. A steady and long-lived dust acoustic wave (DAW) was observed to be spontaneously generated in the cloud; it propagated through the dust cloud, which had a gradually varying density distribution. Two kinds of spectral analyses of the wave motion were performed, using Fourier transforms and Hilbert transforms, respectively; these revealed two distinctive spatial domains in the cloud, termed frequency clusters. Within each cluster, waves were found to oscillate at a dominant frequency that remained constant, manifesting mutual synchronization throughout the cluster. Across the two clusters, the dominant frequency exhibited a step-wise change, with a frequency ratio of 2:1, which is consistent with phase-lock conditions for a harmonic synchronization state.

Ovarian cancer is a gynecological cancer characterized by a high mortality rate and tumor heterogeneity. Its early detection and primary prophylaxis are difficult to perform. Detecting biomarkers for ovarian cancer plays a pivotal role in therapy effectiveness and affects patients’ survival. This study demonstrates the detection of microRNAs (miRNAs), which were reported to be associated with ovarian cancer tumorigenesis, with a nanowire biosensor based on silicon-on-insulator structures (SOI-NW biosensor). The advantages of the method proposed for miRNA detection using the SOI-NW biosensor are as follows: (1) no need for additional labeling or amplification reaction during sample preparation, and (2) real-time detection of target biomolecules. The detecting component of the biosensor is a chip with an array of 3 µm wide, 10 µm long silicon nanowires on its surface. The SOI-NW chip was fabricated using the “top-down” method, which is compatible with large-scale CMOS technology. Oligonucleotide probes (oDNA probes) carrying sequences complementary to the target miRNAs were covalently immobilized on the nanowire surface to ensure high-sensitivity biospecific sensing of the target biomolecules. The study involved two experimental series. Detection of model DNA oligonucleotides being synthetic analogs of the target miRNAs was carried out to assess the method’s sensitivity. The lowest concentration of the target oligonucleotides detectable in buffer solution was 1.1 × 10−16 M. In the second experimental series, detection of miRNAs (miRNA-21, miRNA-141, and miRNA-200a) isolated from blood plasma samples collected from patients having a verified diagnosis of ovarian cancer was performed. The results of our present study represent a step towards the development of novel highly sensitive diagnostic systems for the early revelation of ovarian cancer in women.

Self-propelled colloids, active polymers and membranes, driven (vibrated) granular layers and hybrid synthetic-biological systems are striking examples of systems containing synthetic active Brownian particles. Such particles autonomously convert the available energy of the environment into their own directed mechanical motion. In most studies the self-propelled Brownian particles move in overdamped media. Recently, experiments with Janus particles in a low-pressure plasma have appeared. A distinctive feature of such a medium is an extremely low viscosity at which the inertial effects play a significant role, resulting in underdamped Brownian motion. At present, there is a lack of statistical theory describing the underdamped Brownian motion of self-propelled particles at all time scales. This paper presents the numerical simulation results of active Brownian motion in homogeneous media of different viscosities. The calculations are performed using a mathematical model of a self-propelled Brownian sphere with translational and rotational inertia. The time-dependent mean square displacement and mean linear displacement (the noise-averaged trajectory) of the particle are investigated as a function of medium viscosity, self-propulsion velocity and moment of inertia. Our simulation reveals that the dynamics of a self-propelled spherical particle significantly depends on two independent dimensionless parameters of the particle: the ratio of the self-propulsion velocity to the characteristic thermal velocity and the ratio of the friction coefficient to the rotational diffusion coefficient. The obtained statistical characteristics of active Brownian motion are compared with the known theoretical models in a wide range of medium viscosities. We propose simple corrections to the basic theory of overdamped active Brownian motion, which allow one to calculate the effective diffusion coefficient and the persistence length of a self-propelled Brownian particle in a medium with any dynamic viscosity. The results obtained are discussed in relation to active particles in a colloidal plasma and superfluid helium.

The theoretical and experimental results on plasma currents induced by acoustic-type solitons are generalized. Ion, electron, and dust-acoustic modes in plasma without magnetic field are considered in detail. It is shown that the generation of pulsed plasma currents with a significant direct component is an inherent property of solitons. The basic properties of soliton currents are established.

The main results of a completed cycle of complex studies of a strongly coupled dusty plasma conducted in the unique, experimental Plasma Crystal-3 Plus space laboratory that operated on the International Space Station are discussed. The experiments on the physics of phenomena in the dusty plasma are analyzed. A new state of dusty plasma, the electrorheological plasma, in which there is a transition from an isotropic plasma-dusty liquid to an anisotropic state, is studied. The study of the interpenetration of particles streams of various diameters, a nonequilibrium transition, is described. Experimental studies of the liquid–crystal phase transition in a three-dimensional dusty plasma system in which it is established that the dust component is compressed and crystallized upon a decrease in the neutral gas pressure are discussed. The nearly free motion of large particles in the volume of a plasma crystal, a nonviscous, irrotational flow about a large particle by a “liquid” of small dust particles, is analyzed. An experimental study of the dynamics of the crystallization of three-dimensional plasma-dust systems at a constant argon pressure under the effect of a low-frequency, alternating electric field and without its influence under microgravity conditions is described. The formation of the crystallization front and its propagation in the three-dimensional plasma-dust system with a velocity on the order of the average interparticle distance per second were discovered.

The scientific equipment «Plasmakrystall–4» («PK–4») is designed to study complex (dusty) plasma under microgravity conditions aboard the International Space Station (ISS) and is a joint project of the European Space Agency (ESA) and Roscosmos. Scientific equipment «PK–4» is integrated into «European physiological modules» (EPM) rack, in the European laboratory module Columbus. Experiment control — automated, software-interactive, or manual from an on-board laptop and/or from a terminal in the ground control center. A low-pressure direct current discharge in noble gases in a glass tube is used to create a plasma at scientific equipment «PK–4». Microparticles of a given size are injected into the discharge to obtain a complex plasma. Two digital video cameras allow to trace individual microparticles inside the tube in phase space, which makes a complex plasma to be a good model for studying classical phenomena in condensed matter at the kinetic level. To monitor the plasma conditions, an integrated spectrometer and another video camera are used allowing to observe the plasma's own emission at different wavelengths. To study the reaction of microparticles to external forces, they can be exposed to radiation from a powerful laser, a gas stream, and also to thermophoretic force, i.e., by producing a given temperature gradient. Key words: complex plasmas, microparticles, soft matter, laser manipulation, microgravity, viscosity measurements, gas discharges, plasma diagnostics.

The action of the light pressure force on a bounded region of a 2D system of dust macroparticles is simulated using the molecular dynamics method. The dynamics of dust macroparticles in the quasi-2D structure (trajectories of particles, their mean square displacement, and kinetic energy) is analyzed for various values of the nonideality parameter and laser radiation power. It is shown that by varying the radiation power, it is possible to influence the self-diffusion processes and the value of chaotic velocity of particles. Analysis is performed for different initial values of the nonideality parameter of the unperturbed dust subsystem. It is found that the interparticle interaction results in an increase in the kinetic energy of particles in the region of action, as well as beyond it.

Complex structures, consisting of a large number of interacting subsystems, have the ability to self-organize and evolve, when the scattering of energy coming from the outside ensures the maintenance of stationary ordered structures with an entropy less than the equilibrium entropy. One of the fundamental problems here is the role of quantum phenomena in the evolution of macroscopic objects. We provide experimental evidence for the active Brownian motion and evolution of structures driven by quantum effects for micron-sized grains levitating in superfluid helium. The active Brownian motion of grains was induced by quantum turbulence during the absorption of laser irradiation by grains. The intensity of Brownian motion associated with quantum vortices increased by 6–7 orders of magnitude compared to the values from the Einstein formula. We observed the grain structures in a state far from thermodynamic equilibrium and their evolution to more complex organized structures with lower entropy due to the quantum mechanism of exceedingly high entropy loss in superfluid helium.

Using the modified method of Brownian dynamics, the dynamics of macroparticles with a uniform metal coating in a plasma-dust monolayer under the action of laser radiation was simulated. The time dependences of the root-mean-square and average linear displacements of particles were calculated for different initial effective parameters of nonideality and different intensities of laser radiation. A relationship was established that connects the effective parameter of nonideality of the dusty plasma system of active particles with the maximum value of the mean linear displacement of particles.

The results of a study on particles’ surfaces after being exposed to the near-electrode region of a radio frequency (RF) discharge are presented. It was experimentally displayed that metal starts being deposited on the surface of particles levitating above the lower electrode of the discharge chamber after switching the RF discharge on. For melamine-formaldehyde (MF) particles, the appearance of an island metal coating is observed after 30 min of plasma exposure. Eroded electrodes and elements of the gas discharge chamber may serve as a source of deposited material. In addition, an analysis of the surface and composition of particles placed on the upper electrode after 6 h of plasma exposure is presented. We reveal that the composition and structure of the particle coating changes during the experiment. The MF particles under exposure become eroded, and needle-like structures containing metals are formed on their surface. We also observe the formation of columnar structures from the products of erosion of electrodes on particles with a metal coating.

The detection of CA 125 protein in buffer solution with a silicon-on-insulator (SOI)-based nanoribbon (NR) biosensor was experimentally demonstrated. In the biosensor, sensor chips, bearing an array of 12 nanoribbons (NRs) with n-type conductance, were employed. In the course of the analysis with the NR biosensor, the target protein was biospecifically captured onto the surface of the NRs, which was sensitized with covalently immobilized aptamers against CA 125. Atomic force microscopy (AFM) and mass spectrometry (MS) were employed in order to confirm the formation of the probe–target complexes on the NR surface. Via AFM and MS, the formation of aptamer–antigen complexes on the surface of SOI substrates with covalently immobilized aptamers against CA 125 was revealed, thus confirming the efficient immobilization of the aptamers onto the SOI surface. The biosensor signal, resulting from the biospecific interaction between CA 125 and the NR-immobilized aptamer probes, was shown to increase with an increase in the target protein concentration. The minimum detectable CA 125 concentration was as low as 1.5 × 10−17 M. Moreover, with the biosensor proposed herein, the detection of CA 125 in the plasma of ovarian cancer patients was demonstrated.

Studies of dusty plasma have been actively carried out for about a quarter of a century in many laboratories on Earth, and on board the International Space Station under microgravity conditions. At present, a new experiment is being prepared. We describe the goals and objectives of the experiment, its features and differences from previous experiments, explain the need for it in microgravity. The objective of the experiment is to study the evolution of the open dissipative systems of strongly interacting macroparticles in a gas-discharge plasma, to obtain data on phase transitions and dynamics of active Brownian particles and dynamics of defects and dislocations in plasma-dust structures. Key words: dusty plasma, microgravity, cosmic experiment, open thermodynamic systems, active Brownian particles

We discuss the possibility of using static magnetic traps as an alternative to electrostatic traps for forming and confining structures of charged dust particles in a gas discharge plasma in the context of our study of strongly interacting Coulomb systems. Some advantages of confining structures in magnetic traps over electrostatic ones are shown. Also we provide a review of the related researches carried out first in laboratory conditions, and then under microgravity conditions including the motivation of performing the experiments aboard the International Space Station (ISS). The preparations of a new space experiment «Coulomb-magnet» as well as the differences of a new equipment from previously used are described. We proposed the main tasks of the new experiment as a study of the dynamics and structure of active monodisperse and polydisperse macroparticles in an inhomogeneous magnetic field under microgravity conditions, including phase transitions and the evolution of such systems in the kinetic heating of dust particles by laser radiation. Key words: Coulomb structures, magnetic trap, antiprobotron, diamagnetic particles, dust particles, microgravity.

The application of micro-Raman spectroscopy was used for characterization of structural features of the high-k stack (h-k) layer of “silicon-on-insulator” (SOI) nanowire (NW) chip (h-k-SOI-NW chip), including Al2O3 and HfO2 in various combinations after heat treatment from 425 to 1000 °C. After that, the NW structures h-k-SOI-NW chip was created using gas plasma etching optical lithography. The stability of the signals from the monocrine phase of HfO2 was shown. Significant differences were found in the elastic stresses of the silicon layers for very thick (&gt, 200 nm) Al2O3 layers. In the UV spectra of SOI layers of a silicon substrate with HfO2, shoulders in the Raman spectrum were observed at 480–490 cm−1 of single-phonon scattering. The h-k-SOI-NW chip created in this way has been used for the detection of DNA-oligonucleotide sequences (oDNA), that became a synthetic analog of circular RNA–circ-SHKBP1 associated with the development of glioma at a concentration of 1.1 × 10−16 M. The possibility of using such h-k-SOI NW chips for the detection of circ-SHKBP1 in blood plasma of patients diagnosed with neoplasm of uncertain nature of the brain and central nervous system was shown.

The detection of influenza A virions with a nanoribbon detector (NR detector) has been demonstrated. Chips for the detector have been fabricated based on silicon-on-insulator nanoribbon structures (SOI nanoribbon chip), using a complementary metal-oxide-semiconductor (CMOS)-compatible technology—by means of gas-phase etching and standard optical photolithography. The surface of the SOI nanoribbon chip contains a matrix of 10 nanoribbon (NR) sensor elements. SOI nanoribbon chips of n-type conductance have been used for this study. For biospecific detection of target particles, antibodies against influenza virus have been covalently immobilized onto NRs. Influenza A virus detection was performed by real-time registration of the source-drain current through the NRs. The detection of the target viral particles was carried out in buffer solutions at the target particles concentration within the range from 107 to 103 viral particles per milliliter (VP/mL). The lowest detectable concentration of the target viral particles was 6 × 10−16 M (corresponding to 104 VP/mL). The use of solutions containing ~109 to 1010 VP/mL resulted in saturation of the sensor surface with the target virions. In the saturation mode, detection was impossible.

Microparticle suspensions in a polarity-switched discharge plasma of the Plasmakristall-4 facility on board the International Space Station exhibit string-like order. As pointed out in [Phys. Rev. Research 2, 033314 (2020)2643-156410.1103/PhysRevResearch.2.033314], the string-order is subject to evolution on the timescale of minutes at constant gas pressure and constant parameters of polarity switching. We perform a detailed analysis of this evolution using the pair correlations and length spectrum of the string-like clusters (SLCs). Average exponential decay rate of the SLC length spectrum is used as a measure of string order. The analysis shows that the improvement of the string-like order is accompanied by the decrease of the thickness of the microparticle suspension, microparticle number density, and total amount of microparticles in the field of view. This suggests that the observed long-term evolution of the string-like order is caused by the redistribution of the microparticles, which significantly modifies the plasma conditions.

Application of micro-Raman spectroscopy for the monitoring of quality of nanowire sensor chips fabrication has been demonstrated. Nanowire chips have been fabricated on the basis of «silicon-on-insulator» (SOI) structures (SOI-NW chips). The fabrication of SOI-NW chips was performed by optical litography with gas-phase etching. The so-fabricated SOI-NW chips are intended for highly sensitive detection of brain cancer biomarkers in humans. In our present study, two series of experiments have been conducted. In the first experimental series, detection of a synthetic DNA oligonucleotide (oDNA) analogue of brain cancer-associated microRNA miRNA-363 in purified buffer solution has been performed in order to demonstrate the high detection sensitivity. The second experimental series has been performed in order to reveal miRNA-363 itself in real human plasma samples. To provide detection biospecificity, the SOI-NW chip surface was modified by covalent immobilization of probe oligonucleotides (oDNA probes) complementary to the target biomolecules. Using the SOI-NW sensor chips proposed herein, the concentration detection limit of the target biomolecules at the level of 3.3 × 10−17 M has been demonstrated. Thus, the approach employing the SOI-NW chips proposed herein represents an attractive tool in biomedical practice, aimed at the early revelation of oncological diseases in humans.

We present a detailed analysis of experimental study, which shows clear evidence of a two-stage melting process of a quasi-two-dimensional dusty plasma system in a high-frequency gas discharge. We accurately calculated global parameters of the orientational and translational order, as well as their susceptibilities to determine two critical points, related to “solid-to-hexatic” and “hexatic-to-liquid” phase transitions. The nature of the emerging defects and changes in their mutual concentration, in addition to the estimate of core energy of free dislocations also counts in favor of the formation of an intermediate hexatic phase. These results are fully consistent with the Berezinsky–Kosterlitz–Thouless theory.

Time-dependent shear motion of dust particles in a three-dimensional (3-D) strongly coupled dusty plasma under microgravity conditions was investigated using the European Space Agency–Roscosmos facility Plasma-Kristall 4 (PK-4) on the International Space Station (ISS). The dust particles, which were negatively charged, were trapped in a glow discharge plasma powered by a dc voltage that switched its polarity periodically. They self-organized themselves into a structure resembling a cold liquid. A manipulation laser beam of a circular cross section pushed a stream of particles, moving them through the surrounding sample region; its power was modulated using a rectangular pulse, causing an impulsive unsteady flow. At the onset of the impulsive motion, the flow was observed as it grew in space and time. Later, during the steady manipulation, we find the thickness of the flow region fluctuated significantly over time.

The self-organization of dust grains into stable filamentary dust structures (or "chains") largely depends on dynamic interactions between the individual charged dust grains and the complex electric potential arising from the distribution of charges within the local plasma environment. Recent studies have shown that the positive column of the gas discharge plasma in the Plasmakristall-4 (PK-4) experiment onboard the International Space Station (ISS) supports the presence of fast-moving ionization waves, which lead to variations of plasma parameters by up to an order of magnitude from the average background values. The highly-variable environment resulting from ionization waves may have interesting implications for the dynamics and self-organization of dust particles, particularly concerning the formation and stability of dust chains. Here we investigate the electric potential surrounding dust chains in the PK-4 by employing a molecular dynamics model of the dust and ions with boundary conditions supplied by a Particle-in-Cell with Monte Carlo collisions (PIC-MCC) simulation of the ionization waves. The model is used to examine the effects of the plasma conditions within different regions of the ionization wave and compare the resulting dust structure to that obtained by employing the time-averaged plasma conditions. Comparison between simulated dust chains and experimental data from the PK-4 shows that the time-averaged plasma conditions do not accurately reproduce observed results for dust behavior, indicating that more careful treatment of plasma conditions in the presence of ionization waves is required. It is further shown that commonly used analytic forms of the electric potential do not accurately describe the electric potential near charged dust grains under these plasma conditions., Comment: 15 pages, 14 figures

Experimental data on the active Brownian motion of single particles in the RF (radio-frequency) discharge plasma under the influence of thermophoretic force, induced by laser radiation, depending on the material and type of surface of the particle, are presented. Unlike passive Brownian particles, active Brownian particles, also known as micro-swimmers, move directionally. It was shown that different dust particles in gas discharge plasma can convert the energy of a surrounding medium (laser radiation) into the kinetic energy of motion. The movement of the active particle is a superposition of chaotic motion and self-propulsion.

Three-dimensional structure of complex (dusty) plasmas was investigated under long-term microgravity conditions in the International-Space-Station-based Plasmakristall-4 facility. The microparticle suspensions were confined in a polarity-switched dc discharge. The experimental results were compared to the results of the molecular dynamics simulations with the interparticle interaction potential represented as a superposition of isotropic Yukawa and anisotropic quadrupole terms. Both simulated and experimental data exhibited qualitatively similar structural features indicating the bulk liquid-like order with the inclusion of solid-like strings aligned with the axial electric field. Individual strings were identified and their size spectrum was calculated. The decay rate of the size spectrum was found to decrease with the enhancement of string-like structural features.

We present the results of an experimental study of the behavior of a colloidal plasma system formed by copper-coated and uncoated polymer particles under the action of laser irradiation. A comparative study of particle velocity distribution profiles depending on the power of the pushing laser was conducted. In the case of uncoated melamine-formaldehyde (MF) particles, we observed the well-known action of light pressure, causing shear stress in the colloidal plasma structure and leading to the occurrence of a laminar flow within the affected area. For the copper-coated MF particles, we revealed some additional patterns of behavior for the dust particles, i.e., kinetic temperature growth due to laser radiation absorption by the copper coating, as well as the appearance of chaotic particle motion. We believe that this happens due to the existence of defects in the coating, causing asymmetric heating of the particles, which in turn leads to chaotic deviations of the photophoretic force pushing the particles in different directions.