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Medical application of femtosecond laser irradiation for possible cancer treatment is a primary interest for this study. In this study the effects of femtosecond laser irradiation time, treatment wavelength and strain type upon the structure of DNA from methicillin-resistant Staphylococcus aureus (strain I) and methicillin-susceptible Staphylococcus aureus (strain II) is presented using Fourier transform infrared (FT-IR) spectroscopy. Six sets of irradiation parameters are considered for the bacterial strains. Fourier transform infrared spectra of genomic nucleic acids extracted from the two Staphylococcus aureus bacterial strains were characterized for control and irradiated samples. FT-IR assignments and structure of nucleic acids subgroups were analyzed from 800 to 1800 cm−1. In particular, the bands at 1074 cm−1 [PO2− symmetric stretching (υsPO2−)], 1233 cm−1 (antisymmetric stretching υaPO2−, possible A-form), 1330 cm−1 [deoxyadenosine (dA)], 1400 cm−1 (C3'-endo/anti, A-form), 1540 cm−1 [deoxycytidine (dC)] and 1650 cm−1 [deoxycytidine (dC) (C2 = O2)] were considered to evaluate the structural changes of DNA functional groups following irradiation. [ABSTRACT FROM AUTHOR]

Vibrational properties and structural behavior upon ultraviolet (UV) irradiation are reported here for DNAs isolated from three serovars of Salmonella bacteria, respectively (S. enteritidis, S. infantis and S. typhimurium) by using Fourier transform infrared spectroscopy (FT-IR). The FT-IR absorbance spectra were recorded for bacterial DNAs, control and UV irradiated samples (30 min, 1 h, 1 h 30 min), respectively, and are presented in the wavenumber ranges from 800 to 1800 cm−1 and from 3000 to 4000 cm−1, respectively. Molecular specific structural information has been found based on DNA marker bands, which were monitored primarily in terms of intensity changes. As a general remark, a serovar's dependent nonlinear behavior has been detected for some FT-IR band intensities corresponding to Salmonella DNAs, as a function of irradiation time. Particularly, alterations in DNA functional groups and molecular geometry have been observed. Chemometric analysis such as principal component analysis (PCA) and hierarchical clustering analysis (HCA) were successfully applied for the identification of inter- and intra- serovar differences between DNA spectra, before and after UV irradiation. The elucidation of the main spectral features characteristic to pathogenic bacterial DNAs offers further opportunity for bacteria identification and for establishing the mechanisms of infections, leading to the development of innovative diagnosis tools and more effective therapies. Particularly, DNA-damaging agents are most effective in cancer therapy. [ABSTRACT FROM AUTHOR]