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Your search keyword '"Okuno, Keisuke"' showing total 445 results
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445 results on '"Okuno, Keisuke"'

1. Highly sensitive detection of Epstein-Barr virus-infected cells by EBER flow FISH

Author

Tomomasa, Dan, Tanita, Kay, Hiruma, Yuriko, Hoshino, Akihiro, Kudo, Ko, Azumi, Shohei, Shiota, Mitsutaka, Yamaoka, Masayoshi, Eguchi, Katsuhide, Ishimura, Masataka, Tanaka, Yuka, Iwatsuki, Keiji, Okuno, Keisuke, Hama, Asahito, Sakamoto, Ken-Ichi, Taga, Takashi, Goto, Kimitoshi, Ota, Haruka, Ichiki, Akihiro, Kanda, Kaori, Miyamura, Takako, Endo, Saori, Ohnishi, Hidenori, Sasahara, Yoji, Arai, Ayako, Fornier, Benjamin, Imadome, Ken-Ichi, Morio, Tomohiro, Latour, Sylvain, and Kanegane, Hirokazu

Published
2024
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3. A liquid biopsy signature for predicting early recurrence in patients with gastric cancer.

Author

Okuno, Keisuke, Watanabe, Shuichi, Kanda, Mitsuro, Tokunaga, Masanori, Kodera, Yasuhiro, Kinugasa, Yusuke, Goel, Ajay, and Roy, Souvick

Subjects
Humans, Stomach Neoplasms, Gene Expression Profiling, Biomarkers, Tumor, MicroRNAs, Prognosis, Liquid Biopsy
Abstract

BACKGROUND: Gastric cancer (GC) patients who experience recurrence within the first year following surgery (early recurrence [ER]) exhibit worse prognosis. Herein, we established a microRNA-based liquid biopsy assay to predict ER in GC patients. METHODS: A comprehensive biomarker discovery was performed by analysing miRNA expression profiling in 271 primary GC tumours. Thereafter, the expression of these biomarkers was validated in 290 GC cases, which included 218 tissues and 72 pre-treatment sera, from two independent institutions. RESULTS: A panel of 8 miRNAs was identified during the initial biomarker discovery, and this panel could robustly predict ER in a tissue-based clinical cohort (area under the curve [AUC]: 0.81). Furthermore, a model combining the miRNA panel, microsatellite instability (MSI) status and tumour size exhibited superior predictive performance (AUC: 0.86), and was defined as a Prediction of Early Recurrence in GC (PERGC) signature, which was successfully validated in another independent cohort (AUC: 0.82). Finally, the PERGC signature was translated into a liquid biopsy assay (AUC: 0.81), and a multivariate regression analysis revealed this signature to be an independent predictor for ER (odds ratio: 11.20). CONCLUSION: We successfully established a miRNA-based liquid biopsy signature that robustly predicts the risk of ER in GC patients.

Published
2023

16. An exosome‐based liquid biopsy signature for therapeutic response prediction in metastatic gastric cancer.

Author

Okuno, Keisuke, Watanabe, Shuichi, Hur, Hoon, Lee, Jeeyun, Park, Joon Oh, Tokunaga, Masanori, Tanabe, Minoru, Kinugasa, Yusuke, and Goel, Ajay

Subjects
*EPIDERMAL growth factor receptors, *MITOGEN-activated protein kinases, *TRANSFORMING growth factors, *CANCER chemotherapy, *RECEIVER operating characteristic curves
Abstract

This article discusses a study that aimed to develop a liquid biopsy assay using exosomal microRNAs (exo-miRNAs) to predict the response to first-line systemic chemotherapy in patients with metastatic gastric cancer (mGC). The study involved a biomarker discovery phase, biomarker training, and biomarker validation. The exo-miRNA panel demonstrated a robust ability to predict non-responders to chemotherapy, and it was found to be an independent predictor for lack of response in patients with mGC. The study also confirmed the functional relevance of the miRNA biomarkers and highlighted their potential in precision medicine for mGC. However, the study had limitations in terms of sample size and patient population, and further validation is needed. [Extracted from the article]

Published
2024
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17. Difference in TMPRSS2 usage by Delta and Omicron variants of SARS-CoV-2: Implication for a sudden increase among children.

Author

Kakee, Sosuke, Kanai, Kyosuke, Tsuneki-Tokunaga, Akeno, Okuno, Keisuke, Namba, Noriyuki, Tomita, Katsuyuki, Chikumi, Hiroki, and Kageyama, Seiji

Subjects
SARS-CoV-2 Omicron variant, SARS-CoV-2 Delta variant, CHILD patients, RESPIRATORY organs, COVID-19 pandemic, INDIGENOUS peoples, SUDDEN death
Abstract

It has been postulated from a combination of evidence that a sudden increase in COVID-19 cases among pediatric patients after onset of the Omicron wave was attributed to a reduced requirement for TMPRSS2-mediated entry in pediatric airways with lower expression levels of TMPRSS2. Epidemic strains were isolated from the indigenous population in an area, and the levels of TMPRSS2 required for Delta and Omicron variants were assessed. As a result, Delta variants proliferated fully in cultures of TMPRSS2-positive Vero cells but not in TMPRSS2-negative Vero cell culture (350-fold, Delta vs 9.6-fold, Omicron). There was no obvious age-dependent selection of Omicron strains affected by the TMPRSS2 (9.6-fold, Adults vs. 12-fold, Children). A phylogenetic tree was generated and Blast searches (up to 100 references) for the spread of strains in the study area showed that each strain had almost identical homology (>99.5%) with foreign isolates, although indigenous strains had obvious differences from each other. This suggested that the differences had been present abroad for a long period. Therefore, the lower requirement for TMPRSS2 by Omicron strains might be applicable to epidemic strains globally. In conclusion, the property of TMPRSS2-independent cleavage makes Omicron proliferate with ease and allows epidemics among children with fewer TMPRSS2 on epithelial surfaces of the respiratory organs. [ABSTRACT FROM AUTHOR]

Published
2024
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22. Reticulocyte hemoglobin equivalent is an easily applicable marker for detecting iron deficiency in patients with acute decompensated heart failure

Author

Okuno, Keisuke, primary, Naito, Yoshiro, additional, Ohno, Junichi, additional, Sunayama, Isamu, additional, Matsumoto, Yuki, additional, Soyama, Yuko, additional, Oboshi, Makiko, additional, Manabe, Eri, additional, Azuma, Kohei, additional, Sugahara, Masataka, additional, Min, Kyung-Duk, additional, Asakura, Masanori, additional, and Ishihara, Masaharu, additional

Published
2023
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40. A microRNA signature for risk-stratification and response prediction to FOLFOX-based adjuvant therapy in stage II and III colorectal cancer

Author

Medicina, Medikuntza, Okuno, Keisuke, Kandimalla, Raju, Mendiola, Marta, Balaguer, Francesc, Bujanda Fernández de Pierola, Luis, Fernández Martos, Carlos, Aparicio, Jorge, Feliú, jaime, Tokunaga, Masanori, Kinugasa, Yusuke, Maurel, Joan, Goel, Ajay, Medicina, Medikuntza, Okuno, Keisuke, Kandimalla, Raju, Mendiola, Marta, Balaguer, Francesc, Bujanda Fernández de Pierola, Luis, Fernández Martos, Carlos, Aparicio, Jorge, Feliú, jaime, Tokunaga, Masanori, Kinugasa, Yusuke, Maurel, Joan, and Goel, Ajay

Published
2023

42. A subset of diffuse-type gastric cancer is susceptible to mTOR inhibitors and checkpoint inhibitors

Author

Fukamachi, Hiroshi, Kim, Seon-Kyu, Koh, Jiwon, Lee, Hye Seung, Sasaki, Yasushi, Yamashita, Kentaro, Nishikawaji, Taketo, Shimada, Shu, Akiyama, Yoshimitsu, Byeon, Sun-ju, Bae, Dong-Hyuck, Okuno, Keisuke, Nakagawa, Masatoshi, Tanioka, Toshiro, Inokuchi, Mikito, Kawachi, Hiroshi, Tsuchiya, Kiichiro, Kojima, Kazuyuki, Tokino, Takashi, Eishi, Yoshinobu, Kim, Yong Sung, Kim, Woo Ho, Yuasa, Yasuhito, and Tanaka, Shinji

Published
2019
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45. Additional file 6 of An exosome-based liquid biopsy signature for pre-operative identification of lymph node metastasis in patients with pathological high-risk T1 colorectal cancer

Author

Miyazaki, Katsuki, Wada, Yuma, Okuno, Keisuke, Murano, Tatsuro, Morine, Yuji, Ikemoto, Tetsuya, Saito, Yu, Ikematsu, Hiroaki, Kinugasa, Yusuke, Shimada, Mitsuo, and Goel, Ajay

Abstract

Additional file 6: Supplemental Figure 3. A nomogram illustrates the probability of lymph node metastasis risk.

Published
2023
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47. Additional file 2 of An exosome-based liquid biopsy signature for pre-operative identification of lymph node metastasis in patients with pathological high-risk T1 colorectal cancer

Author

Miyazaki, Katsuki, Wada, Yuma, Okuno, Keisuke, Murano, Tatsuro, Morine, Yuji, Ikemoto, Tetsuya, Saito, Yu, Ikematsu, Hiroaki, Kinugasa, Yusuke, Shimada, Mitsuo, and Goel, Ajay

Abstract

Additional file 2: Supplemental Table 1. Clinicopathological factors of clinical cohorts.

Published
2023
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48. Additional file 5 of An exosome-based liquid biopsy signature for pre-operative identification of lymph node metastasis in patients with pathological high-risk T1 colorectal cancer

Author

Miyazaki, Katsuki, Wada, Yuma, Okuno, Keisuke, Murano, Tatsuro, Morine, Yuji, Ikemoto, Tetsuya, Saito, Yu, Ikematsu, Hiroaki, Kinugasa, Yusuke, Shimada, Mitsuo, and Goel, Ajay

Abstract

Additional file 5: Supplemental Figure 2. Validation of novel combination panel in predicting lymph node metastasis from high-risk T1 CRC patients. Left chart) A receiver operating characteristic curve analysis to evaluate the performance of exosomal and cell-free miRNAs combination panel in a validation cohort. Right chart) Waterfall plot for modified risk score distribution in a validation cohort. LNN: lymph node metastasis negative, LNP: lymph node metastasis positive, AUC: area under the curve.

Published
2023
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50. Additional file 4 of An exosome-based liquid biopsy signature for pre-operative identification of lymph node metastasis in patients with pathological high-risk T1 colorectal cancer

Author

Miyazaki, Katsuki, Wada, Yuma, Okuno, Keisuke, Murano, Tatsuro, Morine, Yuji, Ikemoto, Tetsuya, Saito, Yu, Ikematsu, Hiroaki, Kinugasa, Yusuke, Shimada, Mitsuo, and Goel, Ajay

Abstract

Additional file 4: Supplemental Figure 1. Comparison of diagnostic ability of four cell-free miRNAs and four exosomal miRNAs in lymph node metastasis detection. A) A receiver operating characteristic (ROC) curve analysis to evaluate the performance of four cell-free miRNAs. B) ROC curve analysis to evaluate the performance of four exosomal miRNAs. Cf-: cell-free, exo: exosomal, AUC: area under the curve.

Published
2023
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