1. Comparison of Serologic Assays for Middle East Respiratory Syndrome Coronavirus
- Author
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Harvey, Ruth, Mattiuzzo, Giada, Hassall, Mark, Sieberg, Andrea, Müller, Marcel A., Drosten, Christian, Rigsby, Peter, Caly, L., Li, C., Zhao, L., Tan, W., Peiris, M., Perera, M., Kang, C., Wang, J. S., Haagmans, B., Okba, N. M.A., Gopal, R., Myhill, S., Thornburg, N., and Virology
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Oman ,Comparison of Serologic Assays for Middle East Respiratory Syndrome Coronavirus ,Epidemiology ,viruses ,lcsh:Medicine ,serology ,Fluorescent Antibody Technique ,medicine.disease_cause ,Antibodies, Viral ,Serology ,COVID-19 ,Saudi Arabia ,standard ,South Korea ,antibodies ,MERS-CoV ,diagnostics ,Coronavirus ,Middle East respiratory syndrome coronavirus ,Human health ,0302 clinical medicine ,030212 general & internal medicine ,Oligonucleotide Array Sequence Analysis ,Transmission (medicine) ,virus diseases ,Reference Standards ,3. Good health ,Infectious Diseases ,Middle East Respiratory Syndrome Coronavirus ,Coronavirus Infections ,Microbiology (medical) ,030231 tropical medicine ,Enzyme-Linked Immunosorbent Assay ,Sensitivity and Specificity ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,SDG 3 - Good Health and Well-being ,Neutralization Tests ,medicine ,Humans ,lcsh:RC109-216 ,Serologic Tests ,business.industry ,Research ,lcsh:R ,Outbreak ,Reproducibility of Results ,spike ,Virology ,respiratory tract diseases ,Healthcare settings ,business - Abstract
Middle East respiratory syndrome coronavirus (MERSCoV) was detected in humans in 2012. Since then, sporadic outbreaks with primary transmission through dromedary camels to humans and outbreaks in healthcare settings have shown that MERS-CoV continues to pose a threat to human health. Several serologic assays for MERS-CoV have been developed globally. We describe a collaborative study to investigate the comparability of serologic assays for MERS-CoV and assess any benefit associated with the introduction of a standard reference reagent for MERS-CoV serology. Our study findings indicate that, when possible, laboratories should use a testing algorithm including >2 tests to ensure correct diagnosis of MERS-CoV. We also demonstrate that the use of a reference reagent greatly improves the agreement between assays, enabling more consistent and therefore more meaningful comparisons between results.
- Published
- 2019
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