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Your search keyword '"Okano, Masaharu"' showing total 94 results
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94 results on '"Okano, Masaharu"'

18. Paralogs of Common Carp Granulocyte Colony-Stimulating Factor (G-CSF) Have Different Functions Regarding Development, Trafficking and Activation of Neutrophils

Author

Katakura, Fumihiko, Nishiya, Kohei, Wentzel, Annelieke S., Hino, Erika, Miyamae, Jiro, Okano, Masaharu, Wiegertjes, Geert F., Moritomo, Tadaaki, Katakura, Fumihiko, Nishiya, Kohei, Wentzel, Annelieke S., Hino, Erika, Miyamae, Jiro, Okano, Masaharu, Wiegertjes, Geert F., and Moritomo, Tadaaki

Abstract

Mammalian granulocyte colony-stimulating factor (G-CSF; CSF3) is a primary cytokine that promotes the development, mobilization, and activation of neutrophils and their precursors. Teleosts have been reported to possess two paralogs as a likely result of the teleost-wide whole genome duplication (WGD) event, but functional divergence of G-CSF paralogs remains poorly understood. Common carp are an allotetraploid species owing to an additional WGD event in the carp lineage and here, we report on genomic synteny, sequence similarity, and phylogeny of four common carp G-CSF paralogs (g-csfa1 and g-csfa2; g-csfb1 and g-csfb2). G-csfa1 and g-csfa2 show differential and relatively high gene expression levels, while g-csfb1 and g-csfb2 show low basal gene expression levels in most tissues. All paralogs are expressed higher in macrophages than in other leukocyte sub-types and are highly up-regulated by treatment of macrophages with mitogens. Recombinant G-CSFa1 and G-CSFb1 both promoted the proliferation of kidney hematopoietic cells, while only G-CSFb1 induced the differentiation of kidney cells along the neutrophil-lineage. Colony-forming unit assays revealed that G-CSFb1 alone stimulates the formation of CFU-G colonies from head- and trunk-kidney whereas the combination of G-CSFa1 and G-CSFb1 stimulates the formation of both CFU-G and CFU-GM colonies. Recombinant G-CSFa1 and G-CSFb1 also exhibit chemotactic activity against kidney neutrophils and up-regulation of cxcr1 mRNA expression was highest in neutrophils after G-CSFb1 stimulation. Furthermore, G-CSFb1 more than G-CSFa1 induced priming of kidney neutrophils through up-regulation of a NADPH-oxidase component p47 phox . In vivo administration of G-CSF paralogs increased the number of circulating blood neutrophils of carp. Our findings demonstrate that gene duplications in teleosts can lead to functional divergence between paralogs and shed light on the sub-functionalization of G-CSF paralogs in cyprinid fish.

Published
2019

23. Identification of novel polymorphisms and two distinct haplotype structures in dog leukocyte antigen class I genes: <italic>DLA-88</italic>, <italic>DLA-12</italic> and <italic>DLA-64</italic>.

Author

Miyamae, Jiro, Suzuki, Shingo, Katakura, Fumihiko, Uno, Sae, Tanaka, Mizuki, Okano, Masaharu, Matsumoto, Taro, Kulski, Jerzy K., Moritomo, Tadaaki, and Shiina, Takashi

Subjects
GENETIC polymorphisms, HAPLOTYPES, DOG diseases, SINGLE nucleotide polymorphisms, ANIMAL genetics, LEUCOCYTES
Abstract

The current information on the polymorphism variation and haplotype structure of the domestic dog leukocyte antigen (DLA) genes is limited in comparison to other experimental animals. In this paper, to better elucidate the degree and types of polymorphisms and genetic differences for DLA-88, DLA-12 and DLA-64, we genotyped four families of 38 beagles and another 404 unrelated dogs representing 49 breeds by RT-PCR based Sanger sequencing. We also sequenced and analyzed the genomic organization of the DLA-88 and DLA-12 gene segments to better define these two-gene DLA haplotypes more precisely. We identified 45 alleles for DLA-88, 15 for DLA-12 and six for DLA-64, of which 20, 14 and six, respectively, were newly described alleles. Therefore, this study shows that the DLA-12 and DLA-64 loci are far more polymorphic than previously reported. Phylogenetic analysis strongly supported that the DLA-88, DLA-12 and DLA-64 alleles were independently generated after the original divergence of the DLA-79 alleles. Two distinct DLA-88 and DLA-12 haplotype structures, tentatively named DLA-88-DLA-12 and DLA-88-DLA-88L, were identified, and the novel haplotype DLA-88-DLA-88L contributed to 32.7% of the unrelated dogs. Quantitative real-time PCR analysis showed that the gene expression levels of DLA-88L and DLA-88 were similar, and that the gene expression level of DLA-12 was significantly lower. In addition, haplotype frequency estimations using frequently occurring alleles revealed 45 different DLA-class I haplotypes (88-88L/12-64) overall, and 22 different DLA-class I haplotypes in homozygous dogs for 18 breeds and mongrels. [ABSTRACT FROM AUTHOR]

Published
2018
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24. Radiation measurements on commercial aircrafts and ER-2 at high altitude using Liulin-4J silicon spectrometer

Author

Uchihori, Yukio, Kitamura, Hisashi, Okano, Masaharu, Benton, Eric, and Dachev, Tsvetan

Abstract

In order to consider radiation protection for the aircrews in commercial airline, many measurements were performed by mainly European researchers. And also they have developed several calculation codes based on these measurements and several airlines are using these calculation codes to assess personal doses of aircrews. But these measurements were performed in European or Canadian airlines and there are no organized measurements in Japan. In order to confirm the results of the calculation codes and ensure sudden increasing of radiation doses caused by solar flare or coronal mass ejection, we have performed radiation measurements in commercial aircrafts from and to Japanese airports using the Liulin-4J silicon spectrometers and other detectors. In the future, a supersonic aircraft may be started their service. Because the radiation in the supersonic aircraft must be higher dose rate, it should be investigated. The ER-2 research aircraft in the NASA flied very high altitude till 21 km and we had opportunities to loaded the Liulin-4J in the ER-2 aircraft. We will report these results also., ELEVENTH INTERNATIONAL SCIENTIFIC CONFERENCE, SOLAR-TERRESTRIAL INFLUENCES

Published
2005

25. Space Radiation Measurements by NaI and LiI Scintillation Spectrometers and Liulin-4J Silicon Spectrometer in Japanese Air Lines

Author

Uchihori, Yukio, Fujitaka, Kazunobu, Kitamura, Hisashi, Nojima, Kumie, and Okano, Masaharu

Subjects
Physics::Instrumentation and Detectors, Astrophysics::Instrumentation and Methods for Astrophysics
Abstract

Measurements of space radiation in aircrafts performed in many domestic and international routes. The measurements have been done using NaI Scintillation Spectrometers and LiI Scintillation Spectrometer. There are two types of NaI Scintillation Counters which are 3 inches spherical scintillator and 2 inches cylindrical scintillator which were developed Riken in Japan. One is surrounding LiI scintillation counter, a 5 inches polystyrene sphere is located. Because of this polystyrene sphere, high energy neutrons are moderated to thermal neutrons and the thermal neutrons are converted to 4He and 3H particles and recorded high deposit energy in the LiI scintillation counter. The other is used 2 inches NaI Detector with GPS system. Intensities of space radiation in aircrafts at high altitude can be measured. The ratios of charged particles which were measured in NaI SC and neutron which were measured LiI SC were correlated the altitude of aircraft and geomagnetic latitude. Using Liulin-4J silicon spectrometers which were developed in Bulgaria, a part of charged particles could be observed. The results from Liulin-4J can be compared our NaI SC and LiI Sc. We report these data in many domestic and international routes and results of comparison of these detectors and estimated doses by simulation codes., AOGS, 2nd Annual Meeting 2005

Published
2005

36. Reconstruction procedures of jaw bones by using reimplantation of resected jaw portion treated by freezing and warming.

Author

TSURUKI, Takashi, primary, ISHIKAWA, Nobutaka, additional, ICHINOKAWA, Yoshimi, additional, KURIHARA, Hideyuki, additional, HOMMA, Yoshiaki, additional, SATO, Kazunori, additional, MARUMORI, Masayoshi, additional, SHIBATA, Takanori, additional, OKANO, Masaharu, additional, TAKANO, Nobuo, additional, MATSUI, Takashi, additional, UCHIYAMA, Takeshi, additional, SAITO, Chikara, additional, SHIGEMATSU, Tomohiro, additional, and TAKAHASHI, Shojiro, additional

Published
1985
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40. The Decay of 76Br

Author

Nagahara, Teruaki, primary, Ruan (gen), Jian-zhi, additional, Nakayama, Hiroshi, additional, Ishizuka, Yasuhiro, additional, and Okano, Masaharu, additional

Published
1974
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