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37 results on '"Okamoto, Kazutoshi"'

4. Phenotypic features of dystrophin gene knockout pigs harboring a human artificial chromosome containing the entire dystrophin (DMD) gene

Author

Watanabe, Masahito, Miyamoto, Hitomaru, Okamoto, Kazutoshi, Nakano, Kazuaki, Matsunari, Hitomi, Kazuki, Kanako, Hasegawa, Koki, Uchikura, Ayuko, Takayanagi, Shuko, Umeyama, Kazuhiro, Hiramuki, Yosuke, Kemter, Elisabeth, Klymuik, Nikolai, Kurome, Mayuko, Kessler, Barbara, Wolf, Eckhard, Kazuki, Yasuhiro, and Nagashima, Hiroshi

Published
2023
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9. Phenotypic features of dystrophin gene knockout pigs harboring a human artificial chromosome containing the entire dystrophin gene

Author

Watanabe, Masahito, primary, Miyamoto, Hitomaru, additional, Okamoto, Kazutoshi, additional, Nakano, Kazuaki, additional, Matsunari, Hitomi, additional, Kazuki, Kanako, additional, Hasegawa, Koki, additional, Uchikura, Ayuko, additional, Takayanagi, Shuko, additional, Umeyama, Kazuhiro, additional, Hiramuki, Yosuke, additional, Kemter, Elisabeth, additional, Klymuik, Nikolai, additional, Kurome, Mayuko, additional, Kessler, Barbara, additional, Wolf, Eckhard, additional, Kazuki, Yasuhiro, additional, and Nagashima, Hiroshi, additional

Published
2023
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11. Single‐cell genotyping of phytoplankton from ocean water by gel‐based cell manipulation

Author

Yoshino, Tomoko, primary, Mao, Yihao, additional, Maeda, Yoshiaki, additional, Negishi, Ryo, additional, Murata, Satoshi, additional, Moriya, Seiichiro, additional, Shimada, Hirofumi, additional, Arakaki, Atsushi, additional, Kobayashi, Kenichi, additional, Hagiwara, Yoshitsugu, additional, Okamoto, Kazutoshi, additional, and Tanaka, Tsuyoshi, additional

Published
2022
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13. Genetically engineered pigs manifesting pancreatic agenesis with severe diabetes

Author

Nagaya, Masaki, primary, Hasegawa, Koki, additional, Watanabe, Masahito, additional, Nakano, Kazuaki, additional, Okamoto, Kazutoshi, additional, Yamada, Takeshi, additional, Uchikura, Ayuko, additional, Osafune, Kenji, additional, Yokota, Harumasa, additional, Nagaoka, Taiji, additional, Matsunari, Hitomi, additional, Umeyama, Kazuhiro, additional, Kobayashi, Eiji, additional, Nakauchi, Hiromitsu, additional, and Nagashima, Hiroshi, additional

Published
2020
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16. Generation of heterozygous PKD1mutant pigs exhibiting early-onset renal cyst formation

Author

Watanabe, Masahito, Umeyama, Kazuhiro, Nakano, Kazuaki, Matsunari, Hitomi, Fukuda, Toru, Matsumoto, Kei, Tajiri, Susumu, Yamanaka, Shuichiro, Hasegawa, Koki, Okamoto, Kazutoshi, Uchikura, Ayuko, Takayanagi, Shuko, Nagaya, Masaki, Yokoo, Takashi, Nakauchi, Hiromitsu, and Nagashima, Hiroshi

Abstract

Autosomal dominant polycystic kidney disease (ADPKD) is the most common inherited kidney disease, manifesting as the progressive development of fluid-filled renal cysts. In approximately half of all patients with ADPKD, end-stage renal disease results in decreased renal function. In this study, we used CRISPR-Cas9 and somatic cell cloning to produce pigs with the unique mutation c.152_153insG (PKD1insG/+). Pathological analysis of founder cloned animals and progeny revealed that PKD1insG/+pigs developed many pathological conditions similar to those of patients with heterozygous mutations in PKD1. Pathological similarities included the formation of macroscopic renal cysts at the neonatal stage, number and cystogenic dynamics of the renal cysts formed, interstitial fibrosis of the renal tissue, and presence of a premature asymptomatic stage. Our findings demonstrate that PKD1insG/+pigs recapitulate the characteristic symptoms of ADPKD.

Published
2022
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19. Effectiveness of bioengineered islet cell sheets for the treatment of diabetes mellitus

Author

Nagaya, Masaki, primary, Katsumata, Yuki, additional, Arai, Yoshikazu, additional, Umeki, Ikuma, additional, Nakano, Kazuaki, additional, Kasai, Yuri, additional, Hasegawa, Koki, additional, Okamoto, Kazutoshi, additional, Itazaki, Shiori, additional, Matsunari, Hitomi, additional, Watanabe, Masahito, additional, Umeyama, Kazuhiro, additional, and Nagashima, Hiroshi, additional

Published
2018
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21. Correction: Pigs with δ-sarcoglycan deficiency exhibit traits of genetic cardiomyopathy

Author

Matsunari, Hitomi, Honda, Michiyo, Watanabe, Masahito, Fukushima, Satsuki, Suzuki, Kouta, Miyagawa, Shigeru, Nakano, Kazuaki, Umeyama, Kazuhiro, Uchikura, Ayuko, Okamoto, Kazutoshi, Nagaya, Masaki, Toyo-oka, Teruhiko, Sawa, Yoshiki, and Nagashima, Hiroshi

Abstract

This article was originally published under Nature Research’s License to Publish, but has now been made available under a [CC BY 4.0] license. The PDF and HTML versions of the article have been modified accordingly.

Published
2024
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34. Preference of the grapsid crab hemigrapsus penicillatus (De Haan) for an appropriate aperture.

Author

Kurihara, Yasushi, Okamoto, Kazutoshi, and Takeda, Satoshi

Abstract

The aperture preference of the crab Hemigrapsus penicillatus (De Haan) was investigated in relation to evasion of cannibalism. Crabs migrated into apertures of a certain depth with diameters that fitted their individual body sizes. When crabs were placed artificially into apertures with different diameters, the length of stay was greatest in apertures that were best fitted to body size. These results suggest that small crabs evade predation by larger ones through their preference for apertures best fitted to their body sizes. In addition, subtle differences in preference for the height of a stone substratum and the degree of water turbulence in apertures were observed between adult and juvenile crabs after settling. These differences may decrease the chance of cannibalism by reducing the frequency of encounters between adult and juvenile crabs. [ABSTRACT FROM PUBLISHER]

Published
1989
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35. Skim-Sequencing Based Genotyping Reveals Genetic Divergence of the Wild and Domesticated Population of Black Tiger Shrimp (Penaeus monodon) in the Indo-Pacific Region.

Author

Wong, Li Lian, Deris, Zulaikha Mat, Igarashi, Yoji, Huang, Songqian, Asakawa, Shuichi, Ayub, Qasim, Lim, Shu Yong, Ikhwanuddin, Mhd, Iehata, Shumpei, Okamoto, Kazutoshi, Mariom, and Asaduzzaman, Md

Subjects
PENAEUS monodon, BREEDING, REGULATION of respiration, SINGLE nucleotide polymorphisms, CLUSTER analysis (Statistics), AQUATIC animals, SHRIMPS
Abstract

The domestication of a wild-caught aquatic animal is an evolutionary process, which results in genetic discrimination at the genomic level in response to strong artificial selection. Although black tiger shrimp (Penaeus monodon) is one of the most commercially important aquaculture species, a systematic assessment of genetic divergence and structure of wild-caught and domesticated broodstock populations of the species is yet to be documented. Therefore, we used skim sequencing (SkimSeq) based genotyping approach to investigate the genetic structure of 50 broodstock individuals of P. monodon species, collected from five sampling sites (n = 10 in each site) across their distribution in Indo-Pacific regions. The wild-caught P. monodon broodstock population were collected from Malaysia (MS) and Japan (MJ), while domesticated broodstock populations were collected from Madagascar (MMD), Hawaii, HI, USA (MMO), and Thailand (MT). After various filtering process, a total of 194,259 single nucleotide polymorphism (SNP) loci were identified, in which 4983 SNP loci were identified as putatively adaptive by the pcadapt approach. In both datasets, pairwise FST estimates high genetic divergence between wild and domesticated broodstock populations. Consistently, different spatial clustering analyses in both datasets categorized divergent genetic structure into two clusters: (1) wild-caught populations (MS and MJ), and (2) domesticated populations (MMD, MMO and MT). Among 4983 putatively adaptive SNP loci, only 50 loci were observed to be in the coding region. The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses suggested that non-synonymous mutated genes might be associated with the energy production, metabolic functions, respiration regulation and developmental rates, which likely act to promote adaptation to the strong artificial selection during the domestication process. This study has demonstrated the applicability of SkimSeq in a highly duplicated genome of P. monodon specifically, across a range of genetic backgrounds and geographical distributions, and would be useful for future genetic improvement program of this species in aquaculture. [ABSTRACT FROM AUTHOR]

Published
2020
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