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Additional file 1: View of the white matter (arrow) exposed in the ventricle of a hyh mouse to be dissected out.

Additional file 5: Percentages respect to total number of cells (labelled with DAPI) of mature oligodendrocytes (Olig2-positive and NG2-negative) relative to OPCs (Olig2-positive and NG2-positive). This has been represented for the white matter of wt (n = 6) and hyh (n = 6) mice. * p

Additional file 3: Overexpressed proteins as indicators of astrocyte reaction detected by UHPLC–HRMS. Abundance ratios (Sample hyh) / (Control wt) for GFAP (Gfap, glial fibrillary acidic protein) and vimentin (vim) (wt, n = 4; hyh, n = 5). The sum PEP score corresponds to the score calculated based on the posterior error probability (PEP) values of the peptide spectrum matches (PSM). The PEP indicates the probability that an observed PSM is a random event. Sum PEP score is calculated as the negative logarithms of the PEP values of the connected PSM.

Introduction: In premature newborns, intraventricular hemorrhages (IVH) probably trigger the disruption of the neurogenic ventricular zone. Most of the cases with severe IVH develop post-hemorrhagic hydrocephalus (PHH). A mouse model with IVH has been developed to research into the common neuropathological events present in PHH and into possible therapies. Methods: In two-day-old mice, the blood serum from littermates was injected into the ganglionic eminence of one hemisphere or both hemispheres. Fourteen days later, a histopathological analysis was carried out. In the case of injection in one hemisphere, the effects were compared with the contralateral non-injected hemisphere. Results: Mice with IVH developed the following neuropathological effects. The ependyma was found denuded and replaced by reactive astrocytes. A reaction of astrocytes over-expressing aquaporin-4 and of NG2 cells was also found developed in the white matter. Alterations in the neurogenesis were also common in the ventricular zone and in the white matter. Conclusions: The animal model of IVH developed shows similar neuropathological events to other forms of congenital hydrocephalus and can be used to research into therapies for PHH. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. PI15/0619 (ISCIII/FEDER) to AJJ.