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Your search keyword '"Ohtsuka, Masanari"' showing total 16 results
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16 results on '"Ohtsuka, Masanari"'

1. A non-invasive system to monitor in vivo neural graft activity after spinal cord injury

Author

Ago, Kentaro, Nagoshi, Narihito, Imaizumi, Kent, Kitagawa, Takahiro, Kawai, Momotaro, Kajikawa, Keita, Shibata, Reo, Kamata, Yasuhiro, Kojima, Kota, Shinozaki, Munehisa, Kondo, Takahiro, Iwano, Satoshi, Miyawaki, Atsushi, Ohtsuka, Masanari, Bito, Haruhiko, Kobayashi, Kenta, Shibata, Shinsuke, Shindo, Tomoko, Kohyama, Jun, Matsumoto, Morio, Nakamura, Masaya, and Okano, Hideyuki

Published
2022
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4. Novel System to Monitor In Vivo Neural Graft Activity After Spinal Cord Injury

Author

Ago, Kentaro, primary, Nagoshi, Narihito, additional, Imaizumi, Kent, additional, Kitagawa, Takahiro, additional, Kawai, Momotaro, additional, Kajikawa, Keita, additional, Shibata, Reo, additional, Kamata, Yasuhiro, additional, Kojima, Kota, additional, Shinozaki, Munehisa, additional, Kondo, Takahiro, additional, Iwano, Satoshi, additional, Miyawaki, Atsushi, additional, Ohtsuka, Masanari, additional, Bito, Haruhiko, additional, Kobayashi, Kenta, additional, Shibata, Shinsuke, additional, Shindo, Tomoko, additional, Kohyama, Jun, additional, Matsumoto, Morio, additional, Nakamura, Masaya, additional, and Okano, Hideyuki, additional

Published
2021
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6. Long-Term Two-Photon Calcium Imaging of Neuronal Populations with Subcellular Resolution in Adult Non-human Primates

Author

Sadakane, Osamu, Masamizu, Yoshito, Watakabe, Akiya, Terada, Shin Ichiro, Ohtsuka, Masanari, Takaji, Masafumi, Mizukami, Hiroaki, Ozawa, Keiya, Kawasaki, Hiroshi, Matsuzaki, Masanori, Yamamori, Tetsuo, Sadakane, Osamu, Masamizu, Yoshito, Watakabe, Akiya, Terada, Shin Ichiro, Ohtsuka, Masanari, Takaji, Masafumi, Mizukami, Hiroaki, Ozawa, Keiya, Kawasaki, Hiroshi, Matsuzaki, Masanori, and Yamamori, Tetsuo

Abstract

Two-photon imaging with genetically encoded calcium indicators (GECIs) enables long-term observation of neuronal activity invivo. However, there are very few studies of GECIs in primates. Here, we report a method for long-term imaging of a GECI, GCaMP6f, expressed from adeno-associated virus vectors in cortical neurons of the adult common marmoset (Callithrix jacchus), a small New World primate. We used a tetracycline-inducible expression system to robustly amplify neuronal GCaMP6f expression and up- and downregulate it for more than 100days. We succeeded in monitoring spontaneous activity not only from hundreds of neurons three-dimensionally distributed in layers 2 and 3 but also from single dendrites and axons inlayer 1. Furthermore, we detected selective activities from somata, dendrites, and axons in the somatosensory cortex responding to specific tactile stimuli. Our results provide a way to investigate the organization and plasticity of cortical microcircuits at subcellular resolution in non-human primates. Long-term two-photon calcium imaging has been challenging in non-human primates. Sadakane etal. use an inducible expression system to visualize cortical neurons in adult marmosets. They show that the same neuronal population can be followed over 100days and neuronal responses to tactile stimulation can be imaged at subcellular resolution.

Published
2015

7. Long-Term Two-Photon Calcium Imaging of Neuronal Populations with Subcellular Resolution in Adult Non-human Primates

Author

Sadakane, Osamu, primary, Masamizu, Yoshito, additional, Watakabe, Akiya, additional, Terada, Shin-Ichiro, additional, Ohtsuka, Masanari, additional, Takaji, Masafumi, additional, Mizukami, Hiroaki, additional, Ozawa, Keiya, additional, Kawasaki, Hiroshi, additional, Matsuzaki, Masanori, additional, and Yamamori, Tetsuo, additional

Published
2015
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8. In Vivo Two-Photon Imaging of Dendritic Spines in Marmoset Neocortex

Author

Sadakane, Osamu, primary, Watakabe, Akiya, additional, Ohtsuka, Masanari, additional, Takaji, Masafumi, additional, Sasaki, Tetsuya, additional, Kasai, Masatoshi, additional, Isa, Tadashi, additional, Kato, Go, additional, Nabekura, Junichi, additional, Mizukami, Hiroaki, additional, Ozawa, Keiya, additional, Kawasaki, Hiroshi, additional, and Yamamori, Tetsuo, additional

Published
2015
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11. Visualization of Cortical Projection Neurons with Retrograde TET-Off Lentiviral Vector

Author

Watakabe, Akiya, primary, Kato, Shigeki, additional, Kobayashi, Kazuto, additional, Takaji, Masafumi, additional, Nakagami, Yuki, additional, Sadakane, Osamu, additional, Ohtsuka, Masanari, additional, Hioki, Hiroyuki, additional, Kaneko, Takeshi, additional, Okuno, Hiroyuki, additional, Kawashima, Takashi, additional, Bito, Haruhiko, additional, Kitamura, Yoshihiro, additional, and Yamamori, Tetsuo, additional

Published
2012
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16. DNA methylation and methyl-binding proteins control differential gene expression in distinct cortical areas of macaque monkey.

Author

Hata K, Mizukami H, Sadakane O, Watakabe A, Ohtsuka M, Takaji M, Kinoshita M, Isa T, Ozawa K, and Yamamori T

Subjects
Animals, DNA Methylation, Endodeoxyribonucleases genetics, Endodeoxyribonucleases metabolism, Female, Macaca fascicularis, Macaca mulatta, Male, Neurons metabolism, Promoter Regions, Genetic, Cerebral Cortex metabolism, Epigenesis, Genetic, Gene Expression Regulation
Abstract

Distinct anatomical regions of the neocortex subserve different sensory modalities and neuronal integration functions, but mechanisms for these regional specializations remain elusive. Involvement of epigenetic mechanisms for such specialization through the spatiotemporal regulation of gene expression is an intriguing possibility. Here we examined whether epigenetic mechanisms might play a role in the selective gene expression in the association areas (AAs) and the primary visual cortex (V1) in macaque neocortex. By analyzing the two types of area-selective gene promoters that we previously identified, we found a striking difference of DNA methylation between these promoters, i.e., hypermethylation in AA-selective gene promoters and hypomethylation in V1-selective ones. Methylation levels of promoters of each area-selective gene showed no areal difference, but a specific methyl-binding protein (MBD4) was enriched in the AAs, in correspondence with expression patterns of AA-selective genes. MBD4 expression was mainly observed in neurons. MBD4 specifically bound to and activated the AA-selective genes both in vitro and in vivo. Our results demonstrate that methylation in the promoters and specific methyl-binding proteins play an important role in the area-selective gene expression profiles in the primate neocortex.

Published
2013
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