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44 results on '"Ohiolei, John Asekhaen"'

1. Update on the genetic diversity and population structure of Echinococcus granulosus in Gansu Province, Tibet Autonomous Region, and Xinjiang Uygur Autonomous Region, Western China, inferred from mitochondrial cox1, nad1, and nad5 sequences

Author

Shumuye, Nigus Abebe, Li, Li, Ohiolei, John Asekhaen, Qurishi, Sayed Ajmal, Li, Wen-Hui, Zhang, Nian-Zhang, Wu, Yan-Tao, Wu, Yao-Dong, Gao, Sheng-Zhi, Zhang, Fu-Heng, Tian, Xue-Qi, Tian, Wen-Jun, Fu, Yong, Wang, Xie-Zhong, Pan, Yong-Hong, Zhan, Fang, Zhang, Lin-Sheng, Guo, Ming-Kuan, Li, Wen-Dong, Fu, Bao-Quan, Yan, Hong-Bin, and Jia, Wan-Zhong

Published
2023
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5. Establishment of a secondary infection laboratory model of Echinococcus shiquicus metacestode using BALB/c mice and Mongolian jirds (Meriones unguiculatus)

Author

Wu, Yantao, primary, Li, Li, additional, Xu, Fuling, additional, Yan, Hongbin, additional, Ohiolei, John Asekhaen, additional, Shumuye, Nigus Abebe, additional, Nian, Xiaofeng, additional, Li, Wenhui, additional, Zhang, Nianzhang, additional, Fu, Baoquan, additional, and Jia, Wanzhong, additional

Published
2023
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11. Understanding pathogen–host interplay by expression profiles of lncRNA and mRNA in the liver of Echinococcus multilocularis-infected mice

Author

Nian, Xiaofeng, primary, Li, Li, additional, Ma, Xusheng, additional, Li, Xiurong, additional, Li, Wenhui, additional, Zhang, Nianzhang, additional, Ohiolei, John Asekhaen, additional, Li, Le, additional, Dai, Guodong, additional, Liu, Yanhong, additional, Yan, Hongbin, additional, Fu, Baoquan, additional, Xiao, Sa, additional, and Jia, Wanzhong, additional

Published
2022
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13. Molecular Characterization of a New Moniliformis sp. From a Plateau Zokor (Eospalax fontanierii baileyi) in China

Author

Dai, Guo-Dong, Yan, Hong-Bin, Li, Li, Zhang, Lin-Sheng, Liu, Zhan-Long, Gao, Sheng-Zhi, Ohiolei, John Asekhaen, Wu, Yao-Dong, Guo, Ai-Min, Fu, Bao-Quan, and Jia, Wan-Zhong

Subjects
Microbiology (medical), Microbiology
Abstract

In the present study, a new species of the genus Moniliformis species is described taxonomically in the mitochondrial genomic context. The parasite was found in a plateau zokor captured in a high-altitude area of Xiahe County of Gansu Province, China. The mitochondrial (mt) genome length of this new species was 14,066 bp comprising 36 genes and 2 additional non-coding regions (SNR and LNR), without atp8. The molecular phylogeny inferred by the cytochrome c oxidase subunit I gene (cox1) and the18S ribosomal RNA gene (18S rDNA) sequences showed that the parasite as a sister species to other Moniliformis spp. and was named Moniliformis sp. XH-2020. The phylogeny of the concatenated amino acid sequences of the 12 protein-coding genes (PCGs) showed Moniliformis sp. XH-2020 in the same cluster as Macracanthorhynchus hirudinaceus and Oncicola luehei confirming the cox1 and 18S rDNA phylogenetic inference. In addition, the entire mt genome sequenced in this study represents the first in the order Moniliformida, providing molecular material for further study of the phylogeny of the class Archiacanthocephala. Moreover, the species of this class, use arthropods as intermediate hosts and mammals as definitive hosts and are agents of acanthocephaliasis, a zoonosis in humans. Therefore, this study not only expands the host range among potential wild animal hosts for Archiacanthocephalans which is of great ecological and evolutionary significance but also has important significance for the research of zoonotic parasitic diseases.

Published
2022

14. Expansion of Cyclophyllidea Biodiversity in Rodents of Qinghai-Tibet Plateau and the “Out of Qinghai-Tibet Plateau” Hypothesis of Cyclophyllideans

Author

Wu, Yao-Dong, primary, Dai, Guo-Dong, additional, Li, Li, additional, Littlewood, D. Timothy J., additional, Ohiolei, John Asekhaen, additional, Zhang, Lin-Sheng, additional, Guo, Ai-Min, additional, Wu, Yan-Tao, additional, Ni, Xing-Wei, additional, Shumuye, Nigus Abebe, additional, Li, Wen-Hui, additional, Zhang, Nian-Zhang, additional, Fu, Bao-Quan, additional, Fu, Yong, additional, Yan, Hong-Bin, additional, and Jia, Wan-Zhong, additional

Published
2022
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15. Hydatigera taeniaeformis in urban rats (Rattus rattus) in Faisalabad, Pakistan

Author

Alvi, Mughees Aizaz, primary, Li, Li, additional, Ohiolei, John Asekhaen, additional, Qamar, Warda, additional, Saqib, Muhammad, additional, Tayyab, Muhammad Haleem, additional, Altaf, Javaria, additional, Ashfaq, Khurram, additional, Hassan, Ali, additional, Jamal, Muhammad, additional, Wahab, Abdul, additional, Alvi, Anum Aizaz, additional, Usman, Muhammad, additional, Bajwa, Muhammad Rashid Khalid, additional, Fu, Bao-Quan, additional, Yan, Hong-Bin, additional, and Jia, Wan-Zhong, additional

Published
2021
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16. Additional file 9 of A systematic review and meta-analysis on prevalence and distribution of Taenia and Echinococcus infections in Ethiopia

Author

Shumuye, Nigus Abebe, Ohiolei, John Asekhaen, Gebremedhin, Mebrahtu Berhe, Yan, Hong-Bin, Li, Li, Li, Wen-Hui, Zhang, Nian-Zhang, Fu, Bao-Quan, and Jia, Wan-Zhong

Abstract

Additional file 9: Table S9. Characteristics of studies included in the systematic review and meta-analysis (study subject: final hosts). F, female; M, male; B = both male and female; CS, cross sectional; p, prevalence; CI, confidence interval.

Published
2021
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18. Additional file 6 of A systematic review and meta-analysis on prevalence and distribution of Taenia and Echinococcus infections in Ethiopia

Author

Shumuye, Nigus Abebe, Ohiolei, John Asekhaen, Gebremedhin, Mebrahtu Berhe, Yan, Hong-Bin, Li, Li, Li, Wen-Hui, Zhang, Nian-Zhang, Fu, Bao-Quan, and Jia, Wan-Zhong

Abstract

Additional file 6: Table S6. Characteristics of studies included in the systematic review and meta-analysis (study subject: goat). F, female; M, male; B = both male and female; CS, cross sectional; p, prevalence; CI, confidence interval.

Published
2021
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19. Additional file 4 of A systematic review and meta-analysis on prevalence and distribution of Taenia and Echinococcus infections in Ethiopia

Author

Shumuye, Nigus Abebe, Ohiolei, John Asekhaen, Gebremedhin, Mebrahtu Berhe, Yan, Hong-Bin, Li, Li, Li, Wen-Hui, Zhang, Nian-Zhang, Fu, Bao-Quan, and Jia, Wan-Zhong

Abstract

Additional file 4: Table S4. Characteristics of studies included in the systematic review and meta-analysis (study subject: cattle). F, female; M, male; B = both male and female; CS, cross sectional; p, prevalence; CI, confidence interval.

Published
2021
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20. Additional file 2 of A systematic review and meta-analysis on prevalence and distribution of Taenia and Echinococcus infections in Ethiopia

Author

Shumuye, Nigus Abebe, Ohiolei, John Asekhaen, Gebremedhin, Mebrahtu Berhe, Yan, Hong-Bin, Li, Li, Li, Wen-Hui, Zhang, Nian-Zhang, Fu, Bao-Quan, and Jia, Wan-Zhong

Abstract

Additional file 2: Table S2. Characteristics of studies included in the systematic review and meta-analysis (study subject: human). F, female; M, male; B = both male and female; Imm, immigrant; CS, cross sectional; p, prevalence; CI, confidence interval.

Published
2021
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21. Additional file 5 of A systematic review and meta-analysis on prevalence and distribution of Taenia and Echinococcus infections in Ethiopia

Author

Shumuye, Nigus Abebe, Ohiolei, John Asekhaen, Gebremedhin, Mebrahtu Berhe, Yan, Hong-Bin, Li, Li, Li, Wen-Hui, Zhang, Nian-Zhang, Fu, Bao-Quan, and Jia, Wan-Zhong

Abstract

Additional file 5: Table S5. Characteristics of studies included in the systematic review and meta-analysis (study subject: sheep). F, female; M, male; B = both male and female; CS, cross sectional; p, prevalence; CI, confidence interval.

Published
2021
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22. Additional file 3 of A systematic review and meta-analysis on prevalence and distribution of Taenia and Echinococcus infections in Ethiopia

Author

Shumuye, Nigus Abebe, Ohiolei, John Asekhaen, Gebremedhin, Mebrahtu Berhe, Yan, Hong-Bin, Li, Li, Li, Wen-Hui, Zhang, Nian-Zhang, Fu, Bao-Quan, and Jia, Wan-Zhong

Abstract

Additional file 3: Table S3. Distribution of data sets by animal intermediate hosts’ taeniasis and CE, Ethiopia. n, number of report; AA, Addis Ababa; Oro, Oromia; Tig, Tigray; SNNP, Southern Nation and Nationality of People; Amh, Amhara; Har, Harar; DD, Dire Dawa; Som, Somali; *some papers reported more than one parasite hence multiple datasets.

Published
2021
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23. Additional file 8 of A systematic review and meta-analysis on prevalence and distribution of Taenia and Echinococcus infections in Ethiopia

Author

Shumuye, Nigus Abebe, Ohiolei, John Asekhaen, Gebremedhin, Mebrahtu Berhe, Yan, Hong-Bin, Li, Li, Li, Wen-Hui, Zhang, Nian-Zhang, Fu, Bao-Quan, and Jia, Wan-Zhong

Abstract

Additional file 8: Table S8. Characteristics of studies included in the systematic review and meta-analysis (study subject: pig). F, female; M, male; B = both male and female; CS, cross sectional; p, prevalence; CI, confidence interval.

Published
2021
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24. PCR‐RFLP assay confirms the existence of different mitochondrial lineages of Taenia hydatigena including a possible geographically restricted group.

Author

Ohiolei, John Asekhaen, Yan, Hong‐Bin, Li, Li, Alvi, Mughees Aizaz, Muku, Rosline James, Wu, Yao‐Dong, Zhang, Nian‐Zhang, Li, Wen‐Hui, Guo, Ai‐Min, Wang, Xue‐Lin, Fu, Bao‐Quan, and Jia, Wan‐Zhong

Subjects
*TAENIA, *MITOCHONDRIA, *CYSTICERCOSIS, *ANIMAL mortality, *HAPLOGROUPS, *ANIMAL young
Abstract

Taenia hydatigena is a widespread tapeworm of canids (primarily dogs) that causes cysticercosis in ruminants (domestic and wild) and manifests as depression and weakness secondary to various hepatic damages and sometimes mortality in young animals, although, commonly encountered cases are asymptomatic. In most taeniids, genetic polymorphism has been found to impact host preferences, distribution, disease epidemiology and management. Recently, we identified two main mitochondrial lineages of T. hydatigena in China, and here, we examined the mitochondrial nad4‐nad5 genes of T. hydatigena from China, Nigeria, Pakistan and Sudan to assess the intraspecies variation of isolates from these countries and also the distribution of the distinct mitochondrial groups. In addition to China, haplogroup B variant was found in Pakistan, while haplogroup A demonstrated a widespread distribution. We then designed a PCR‐restriction fragment length polymorphism (PCR‐RFLP) assay using XmiI (AccI) and RsaI (AfaI) restriction enzymes to differentiate members of both haplogroups. This result provides more molecular evidence supporting the existence of distinct mitochondrial variants of T. hydatigena. The epidemiological significance of these different mitochondrial groups remains to be explored further. The current PCR‐RFLP assay offers a useful molecular approach for investigating the genetic population structure of T. hydatigena in enzootic regions and in identifying/discriminating the different mitochondrial groups (haplogroups A and B). [ABSTRACT FROM AUTHOR]

Published
2022
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25. Serologic evidence of Echinococcus granulosus in slaughterhouses in Pakistan: global alarm for butchers in developing countries

Author

Alvi, Mughees Aizaz, primary, Li, Li, additional, Saqib, Muhammad, additional, Ohiolei, John Asekhaen, additional, Younas, Muhammad Waqar, additional, Tayyab, Muhammad Haleem, additional, Hassan, Ali, additional, Qamar, Warda, additional, Alvi, Anum Aizaz, additional, Fu, Bao-Quan, additional, Yan, Hong-Bin, additional, and Jia, Wan-Zhong, additional

Published
2021
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26. Understanding Pathogen-host Interplay by Expression Profiles of LncRNA and mRNA in the Liver of Echinococcus Multilocularis-infected Mice

Author

Nian, Xiaofeng, primary, Li, Li, additional, Ma, Xusheng, additional, Li, Xiurong, additional, Li, Wenhui, additional, Zhang, Nianzhang, additional, Ohiolei, John Asekhaen, additional, Li, Le, additional, Dai, Guodong, additional, Yan, Hongbin, additional, Fu, Baoquan, additional, Xiao, Sa, additional, and Jia, Wanzhong, additional

Published
2021
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27. PCR‐RFLP assay confirms the existence of different mitochondrial lineages of Taenia hydatigena including a possible geographically restricted group

Author

Ohiolei, John Asekhaen, primary, Yan, Hong‐Bin, additional, Li, Li, additional, Alvi, Mughees Aizaz, additional, Muku, Rosline James, additional, Wu, Yao‐Dong, additional, Zhang, Nian‐Zhang, additional, Li, Wen‐Hui, additional, Guo, Ai‐Min, additional, Wang, Xue‐Lin, additional, Fu, Bao‐Quan, additional, and Jia, Wan‐Zhong, additional

Published
2021
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28. Echinococcus shiquicus in Qinghai–Tibet plateau: population structure and confirmation of additional endemic areas

Author

Yan, Hong-Bin, primary, Li, Li, additional, Li, Wenhui, additional, Zhu, Guoqiang, additional, Li, Jian-Qiu, additional, Wu, Yantao, additional, Zhang, Nianzhang, additional, Wu, Yaodong, additional, Li, Min, additional, Zhang, Linsheng, additional, Yao, Gang, additional, Tian, Wenjun, additional, Li, Le, additional, Li, Wenjing, additional, Guo, Aimin, additional, Dai, Guodong, additional, Fu, Baoquan, additional, Ohiolei, John Asekhaen, additional, and Jia, Wan-Zhong, additional

Published
2021
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30. Additional file 1 of First report on the phylogenetic relationship, genetic variation of Echinococcus shiquicus isolates in Tibet Autonomous Region, China

Author

Zhu, Guo-Qiang, Yan, Hong-Bin, Li, Li, Ohiolei, John Asekhaen, Wu, Yan-Tao, Li, Wen-Hui, Zhang, Nian-Zhang, Fu, Bao-Quan, and Jia, Wan-Zhong

Abstract

Additional file 1: Fig. S1. Multiplex PCR results image of Echinococcus shiquicus samples based on three specific primers of E. multilocularis, E. shiquicus and E. granulosus.

Published
2020
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31. Additional file 1 of Echinococcus granulosus (sensu stricto) (G1, G3) and E. ortleppi (G5) in Pakistan: phylogeny, genetic diversity and population structural analysis based on mitochondrial DNA

Author

Mughees Aizaz Alvi, Ohiolei, John Asekhaen, Saqib, Muhammad, Li, Li, Tayyab, Muhammad Haleem, Anum Aizaz Alvi, Wu, Yan-Tao, Fu, Bao-Quan, Yan, Hong-Bin, and Jia, Wan-Zhong

Subjects
parasitic diseases
Abstract

Additional file 1: Table S1.Echinococcus granulosus (s.s.) mitochondrial cox1 gene nucleotide sequence polymorphism and corresponding amino acid changes among haplotypes from cattle and buffalo. Table S2.Echinococcus granulosus (s.s.) mitochondrial nad1 gene nucleotide sequence polymorphism and corresponding amino acid changes among haplotypes from cattle and buffalo. Table S3.Echinococcus ortleppi mitochondrial cox1 gene nucleotide sequence polymorphism among haplotypes from cattle. Table S4.Echinococcus ortleppi mitochondrial nad1 gene nucleotide sequence polymorphism among haplotypes from cattle.

Published
2020
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32. First Report on Molecular Characterization of Taenia multiceps Isolates From Sheep and Goats in Faisalabad, Pakistan

Author

Alvi, Mughees Aizaz, primary, Ohiolei, John Asekhaen, additional, Saqib, Muhammad, additional, Tayyab, Muhammad Haleem, additional, Zafar Khan, Muhammad Umar, additional, Li, Li, additional, Aqib, Amjad Islam, additional, Hassan, Ali, additional, Alvi, Anum Aizaz, additional, Qamar, Warda, additional, Fu, Bao-Quan, additional, Yan, Hong-Bin, additional, and Jia, Wan-Zhong, additional

Published
2020
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33. Preliminary information on the prevalence and molecular description of Taenia hydatigena isolates in Pakistan based on mitochondrial cox1 gene

Author

Alvi, Mughees Aizaz, primary, Ohiolei, John Asekhaen, additional, Saqib, Muhammad, additional, Li, Li, additional, Muhammad, Nehaz, additional, Tayyab, Muhammad Haleem, additional, Qamar, Warda, additional, Alvi, Anum Aizaz, additional, Wu, Yao-Dong, additional, Li, Xiu-Rong, additional, Fu, Bao-Quan, additional, Yan, Hong-Bin, additional, and Jia, Wan-Zhong, additional

Published
2020
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34. In-House Developed ELISA Indicates High Prevalence of Anti-Echinococcus granulosus IgG in Sheep Population—An Update from Pakistan

Author

Alvi, Mughees Aizaz, primary, Ohiolei, John Asekhaen, additional, Li, Li, additional, Saqib, Muhammad, additional, Hussain, Muhammad Hammad, additional, Tayyab, Muhammad Haleem, additional, Ghafoor, Muzafar, additional, Qamar, Warda, additional, Faridi, Noman Yousaf, additional, Alvi, Anum Aizaz, additional, Fu, Bao-Quan, additional, Yan, Hong-Bin, additional, and Jia, Wan-Zhong, additional

Published
2020
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37. Trichinella spiralis Thioredoxin Peroxidase 2 Regulates Protective Th2 Immune Response in Mice by Directly Inducing Alternatively Activated Macrophages

Author

Jin, Qi-Wang, primary, Zhang, Nian-Zhang, additional, Li, Wen-Hui, additional, Qin, Hong-Tao, additional, Liu, Yin-Ju, additional, Ohiolei, John Asekhaen, additional, Niu, Dong-Yu, additional, Yan, Hong-Bin, additional, Li, Li, additional, Jia, Wan-Zhong, additional, Song, Ming-Xin, additional, and Fu, Bao-Quan, additional

Published
2020
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42. A new molecular nomenclature for Taenia hydatigena : mitochondrial DNA sequences reveal sufficient diversity suggesting the assignment of major haplotype divisions.

Author

Ohiolei, John Asekhaen, Yan, Hong-Bin, Li, Li, Li, Wen-Hui, Wu, Yao-Dong, Alvi, Mughees Aizaz, Zhang, Nian-Zhang, Fu, Bao-Quan, Wang, Xue-Lin, and Jia, Wan-Zhong

Subjects
*CYSTICERCOSIS, *NUCLEOTIDE sequence, *TAENIA, *MITOCHONDRIAL DNA, *LIVESTOCK productivity, *DNA analysis, *WILD boar
Abstract

Cysticercosis caused by the metacestode larval stage of Taenia hydatigena formerly referred to as Cysticercus tenuicollis is a disease of veterinary importance that constitutes a significant threat to livestock production worldwide, especially in endemic regions due to condemnation of visceral organs and mortality rate of infected young animals. While the genetic diversity among parasites is found to be potentially useful in many areas of research including molecular diagnostics, epidemiology and control, that of T. hydatigena across the globe remains poorly understood. In this study, analysis of the mitochondrial DNA (mtDNA) of adult worms and larval stages of T. hydatigena isolated from dogs, sheep and a wild boar in China showed that the population structure consists of two major haplogroups with very high nucleotide substitutions involving synonymous and non-synonymous changes. Compared with other cestodes such as Echinococcus spp., the genetic variation observed between the haplogroups is sufficient for the assignment of major haplotype or genotype division as both groups showed a total of 166 point-mutation differences between the 12 mitochondrial protein-coding gene sequences. Preliminary analysis of a nuclear protein-coding gene (pepck) did not reveal any peculiar changes between both groups which suggests that these variants may only differ in their mitochondrial makeup. [ABSTRACT FROM AUTHOR]

Published
2021
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44. First Comparative Biochemical Profile Analysis of Cystic Fluids of Taenia hydatigena and Echinococcus granulosus Obtained from Slaughtered Sheep and Goats.

Author

Alvi, Mughees Aizaz, Li Li, Khan Bahadur, Sami Ullah, Saqib, Muhammad, Ohiolei, John Asekhaen, Athar Ali, Rana Muhammad, Rashid, Imaad, Ashfaq, Khurram, Butt, Asif Ali, Abbas, Muhammad Zaeem, Tayyab, Muhammad Haleem, Aqib, Amjad Islam, Qamar, Warda, Hassan, Ali, Alvi, Anum Aizaz, Aslam, Azhar, Bao-Quan Fu, Hong-Bin Yan, and Wan-Zhong Jia

Subjects
*ECHINOCOCCUS granulosus, *TAENIA, *LIVER enzymes, *FLUIDS, *GOATS, *ASPARTATE aminotransferase
Abstract

The organic and inorganic constituents present in cystic fluid play a crucial role in the physiology, metabolism and immune responses mediated by taeniids. We designed a comparative study on T. hydatigena and E. granulosus cysts based on biochemical profile. In the present study, we aspirated fluid from Taenia hydatigena (n=30) and Echinococcus granulosus (n=30) cysts recovered from slaughtered sheep and goats in the central abattoir of Faisalabad, Pakistan. The fluid obtained from each cyst was subjected to biochemical analysis to estimate the levels of liver enzymes, electrolytes and selected biomolecules (proteins, glucose, cholesterol, urea, and creatinine). Thereafter, the student's t-test was used to determine significant differences between constituents of the cystic fluids obtained from both type of cysts. The difference was considered statistically significant at P<0.05. The levels of total protein and globulin differed significantly (P<0.05) and were found to be higher in T. hydatigena cystic fluid. A non-significant difference was observed for albumin. Similarly, higher concentrations of electrolytes (sodium, chloride and magnesium) were observed in T. hydatigena cystic fluid with statistically significant difference (P<0.05). Concerning liver enzymes, high levels (p>0.05) of ALP and LDH were observed in T. hydatigena and E. granulosus cystic fluid had significantly higher (P<0.05) level of AST. The levels of cholesterol, urea and creatinine were higher in E. granulosus. In contrast, glucose was higher in T. hydatigena. A significant difference (P<0.05) was observed for these four biomolecules. Overall, these results suggest high variations of investigated biomolecules, liver enzymes, and electrolytes composition between cystic fluids of E. granulosus and T. hydatigena. Further studies are now warranted to set the standards for the differential diagnosis of cysticercosis and cystic echinococcosis based on cystic fluid contents. [ABSTRACT FROM AUTHOR]

Published
2022
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