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308 results on '"Oh-ishi S"'

1. Tuberculosis in Older Adults: Ibaraki, Japan

Author

Hase, I., primary, Toren, K.G., additional, Hirano, H., additional, Yabuuchi, Y., additional, Kawashima, K., additional, Kubota, S., additional, Shimada, T., additional, Kitaoka, Y., additional, Arai, N., additional, Hyodo, K., additional, Nakazawa, A., additional, Kanazawa, J., additional, Miura, Y., additional, Oh-Ishi, S., additional, Hayashihara, K., additional, Horne, D.J., additional, Saito, T., additional, and Narita, M., additional

Published
2020
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37. Immunomodulation by cells of mononuclear phagocyte lineage in acute cold-stressed or cold-acclimatized mice.

Author

Kizaki, T., Yamashita, H., Oh-Ishi, S., Day, N. K., and Goodt, R. A.

Subjects
HEMATOPOIETIC stem cells, IMMUNOREGULATION, CELL proliferation, PHYSIOLOGICAL effects of cold temperatures, LECTINS, ENDOTOXINS, LABORATORY mice
Abstract

Immunoregulatory states in acute cold-stressed or cold-acclimatized mice were investigated. When male C57BL/6 mice were exposed to environmental temperature of 5° for 24 hr (acute cold stress), the spleen cells showed depressed proliferative responses to stimulation with concanavalin A (Con A) or lipopolysaccharide (LPS) compared with control mice (reared at 25°). The proportion of Thy-1.2+ cells increased significantly in spleens from these acute cold-stressed mice. The Con A responses of T-enriched cells from acute cold-stressed mice were restored to the normal level by adding plastic-adherent cells from control mice. Further, adherent cells from acute cold-stressed mice markedly suppressed the Con A responses of control spleen cells. Thus, the plastic-adherent cells appeared to be responsible for the suppressed Con A responses. On the other hand, proliferative responses to Con A or LPS were elevated in spleen cells from mice exposed to 5° for 3 weeks (cold acclimatization). A significant decrease of Thy-l.2+ cells was detected in these spleens. It was shown that the enhanced proliferative responses were attributable to functional alterations of the plastic adherent cell population but not to those of lymphoid cell population. These findings indicate that the low or high responsiveness of spleen cells to Con A observed in cold-stressed or cold-acclimatized mice, respectively, may be due to a mechanism including the contrary modulations of functions of cells of mononuclear phagocyte lineage. [ABSTRACT FROM AUTHOR]

Published
1995

50. Rat plasma high-molecular-weight kininogen. A simple method for purification and its characterization.

Author

Hayashi, I, Kato, H, Iwanaga, S, and Oh-ishi, S

Abstract

High-molecular-weight kininogen has been isolated from rat plasma in three steps in a relatively high yield. The purified preparation gave a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the absence and presence of 2-mercaptoethanol, and the apparent Mr was estimated as 100,000. On incubation with rat plasma kallikrein, rat high Mr kininogen yielded a kinin-free protein consisting of a heavy chain (Mr = 64,000) and a light chain (Mr = 46,000), liberating bradykinin. The kinin-free protein was S-alkylated, and its heavy and light chains were separated by a zinc-chelating Sepharose 6B column. The amino acid compositions of rat high Mr kininogen and its heavy and light chains were very similar to those of bovine high Mr kininogen and its heavy and fragment 1.2-light chains, respectively. A high histidine content in the light chain of rat high Mr kininogen indicated the presence of a histidine-rich region in this protein as in bovine high Mr kininogen, although this region was not cleaved by rat plasma kallikrein. Rat high Mr kininogen corrected to normal values the prolonged activated partial thromboplastin time of Brown-Norway Katholiek rat plasma known to be deficient in high Mr kininogen and of Fitzgerald trait plasma. The kinin-free protein had the same correcting activity as intact high Mr kininogen. Rat high Mr kininogen also accelerated approximately 10-fold the surface-dependent activation of rat factor XII and prekallikrein, which was mediated with kaolin, amylose sulfate, and sulfatide. These results indicate that rat high Mr kininogen is quite similar to human and bovine high Mr kininogens in terms of biochemical and functional properties.

Published
1985
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