327 results on '"Oh HB"'
Search Results
2. Pseudogout – a rare manifestation of hungry bone syndrome after focused parathyroidectomy
- Author
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Tai, CH, primary, Oh, HB, additional, Seet, JE, additional, and Ngiam, KY, additional
- Published
- 2018
- Full Text
- View/download PDF
3. Ductal carcinoma in-situ arising within benign phyllodes tumours
- Author
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Lui, S-A, primary, Oh, HB, additional, Wang, S, additional, and Chan, CW, additional
- Published
- 2018
- Full Text
- View/download PDF
4. Advanced trauma life support (ATLS®): the ninth edition
- Author
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Chapleau, W, Al khatib, J, Haskin, D, Leblanc, P, Cardenas, G, Borum, S, Torres, N, abi Saad, G, al Ghanimi, O, Al Harthy, A, al turki, S, Ali, J, Allerton, D, Androulakis, Ja, Arca, Mj, Armstrong, Jh, Atkinson, Jl, Ayyaz, M, Baker, A, Blake, Dp, Sallee, R, Scruggs, F, Bowyer, Mw, Brandt, Mm, Branicki, Fj, Brasel, K, Brighton, G, Brown, J, Bruna, L, Burton, Ra, Bustraan, J, Cabading, V, Carvajal Hafemann, C, Castagneto, Gh, Castro, Cl, Chaudhry, Zu, Chehardy, P, Chennault, Rs, Chua, Wc, Chrysos, E, Coimbra, R, Silva F, Collet e., Cooper, A, Cortes Ojeda, J, Cothren Burlew, C, Chetty, D, Davis, Ka, Domingues Cde, A, di Silvio lopez, M, Doucet, Jj, du Plessis HJ, Dunn, Ja, Dyson, R, Dason, M, Eastman, Ab, Elkholy, At, Falck larsen, C, Fernandez, Fa, Foianini, E, Foerster, J, Frankel, H, Gautam, Sc, Gomez, Ga, Gomez Fernandez AH, Guillamondegui, Od, Guzman Cottallat EA, Hancock, Bj, Henn, R, Henny, W, Henry, Sm, Herrera Fernandez, G, Hollands, M, Horbowyj, R, Hults, Cm, Jawa, Rs, Jover Navalon JM, Jurkovich, Gj, Kaufmann, Cr, Knudson, P, Kortbeek, Jb, Kosir, R, Kuncir, Ej, Ladner, R, Lo, Cj, Logsetty, S, Lui, Kk, Lum, Sk, Lundy, Dw, Machado, F, Mao, P, Masood Gondal, K, Maxson, Rt, Mcintyre, C, Michael, Db, Misra, Mc, Moore, Fo, Mori, Nd, Morrow CE Jr, Murphy, Sg, Nagy, Kk, Nicolau, N, Oh, Hb, Omari, Oa, Ong, Hs, Olivero, Giorgio, Pak art, R, Parry, Ng, Patel, Br, Paul, Js, Pereira, Pm, Poggetti, Rs, Poole, A, Recalde Hidrobo, M, Price, Rr, Primeau, S, Quintana, C, Razek, Ts, Roden, R, Roed, J, Romero, M, Rotondo, Mf, Sabahi, M, Schaapveld, N, Schipper, Ib, Schoettker, P, Schreiber, Ma, Serafico, Ec, Serrano, Jc, Siegel, B, Siritongtaworn, P, Skaff, D, Smith, Rs, Sorvari, A, Sutter, Pm, Sutyak, J, Svendsen, Lb, Taha, Ws, Tchorz, K, Lee, Wt, Tisminetzky, G, Trostchansky, Jl, Truskett, P, Upperman, J, van den Ende, Y, Vennike, A, Vikström, T, Voiglio, E, Weireter LJ Jr, Wetjen, Nm, Wigle, Rl, Wilkinson, S, Winchell, Rj, Winter, R, Yelon, Ja, and Zarour, Am
- Subjects
Ninth ,initial assessment ,medicine.medical_specialty ,Education, Medical ,business.industry ,Teaching Materials ,MEDLINE ,Critical Care and Intensive Care Medicine ,Education ,Advanced trauma life support ,Advanced Trauma Life Support Care ,trauma ,ATLS ,Medical ,Family medicine ,app ,elearning ,Curriculum ,Humans ,Surgery ,medicine ,business - Published
- 2013
5. Oseltamivir-resistant pandemic (H1N1) 2009 virus, South Korea.
- Author
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Yi H, Lee JY, Hong EH, Kim MS, Kwon D, Choi JH, Choi WY, Kim KS, Lee JK, Oh HB, Kang C, Yi, Hwajung, Lee, Joo Yeon, Hong, Eun Hye, Kim, Mi Seon, Kwon, Donghyok, Choi, Jang Hoon, Choi, Woo Young, Kim, Ki Soon, and Lee, Jong Koo
- Abstract
To identify oseltamivir resistance, we analyzed neuraminidase H275Y mutations in samples from 10 patients infected with pandemic (H1N1) 2009 virus in South Korea who had influenza that was refractory to antiviral treatment with this drug. A neuraminidase I117M mutation that might influence oseltamivir susceptibility was detected in sequential specimens from 1 patient. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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6. 5-HT2(A) receptor T102C polymorphism and DRD3 Ser9Gly polymorphism is not associated with clinical response to atypical antipsychotics in Korean schizophrenic patients
- Author
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Kim, Bs, Park, Ch, Hwang, Ny, Oh, Hb, Song, Y., Kim, Cy, and Young Hoon Joo
7. Human leukocyte antigen alleles and haplotypes associated with chronicity of hepatitis B virus infection in Koreans.
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Hwang SH, Sohn YH, Oh HB, Hwang CY, Lee SH, Shin ES, and Lee KJ
- Subjects
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HEPATITIS viruses , *CHRONIC hepatitis B , *GLYCOPROTEINS , *RETROSPECTIVE studies , *GENES , *ODDS ratio , *HISTOCOMPATIBILITY antigens , *ALLELES , *GENOTYPES , *HLA-B27 antigen - Published
- 2007
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8. Simple, direct amplification of RNA-containing paper discs for diagnosing the hepatitis C virus.
- Author
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Chu D, Oh YH, Sung H, Ko DH, Oh HB, and Hwang SH
- Subjects
- Humans, Point-of-Care Testing, Limit of Detection, Hepacivirus genetics, Hepacivirus isolation & purification, RNA, Viral genetics, RNA, Viral isolation & purification, Nucleic Acid Amplification Techniques methods, Hepatitis C diagnosis, Hepatitis C virology, Molecular Diagnostic Techniques methods, Sensitivity and Specificity, Paper
- Abstract
Nucleic acid extraction (NAE) is crucial for molecular diagnostics but presents challenges in point-of-care testing (POCT) and decentralized settings. We developed a streamlined, paper-based NAE method for hepatitis C virus (HCV) RNA amplification, suitable for integration into POCT and lab-on-a-chip systems. This method uses Fusion 5 paper discs, completing extraction in under 30 min without centrifugation. The nucleic acids on the disc can be directly used or eluted for amplification. We validated this method's compatibility with reverse transcription-polymerase chain reaction (RT-PCR), real-time quantitative PCR (RQ-PCR), and loop-mediated isothermal amplification (LAMP), demonstrating versatility across amplification platforms. Clinical evaluation (n = 60) showed 100% sensitivity and specificity with a low detection limit of ~10
1 IU/mL. Results matched those from standard HCV RQ-PCR, confirming accuracy. Additionally, incorporating polyethylene glycol (PEG) improves extraction efficiency, eliminating the need for ethanol treatment and washing/drying steps. This modification enhances performance and suitability for field applications. Our paper-based HCV amplification is affordable and user-friendly, making it valuable for decentralized HCV detection and supporting global health initiatives., (© 2024 Wiley Periodicals LLC.)- Published
- 2024
- Full Text
- View/download PDF
9. The Novel HLA-C Allele, HLA-C*14:153, Identified in a Kidney Transplantation Recipient.
- Author
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Yang JJ, Kim SH, and Oh HB
- Subjects
- Humans, Sequence Analysis, DNA methods, Transplant Recipients, Sequence Alignment, Codon, HLA-C Antigens genetics, Kidney Transplantation, Alleles, Exons, Histocompatibility Testing, Base Sequence
- Abstract
HLA-C*14:153 differs from HLA-C*14:02:01:01 by a non-synonymous nucleotide substitution in exon 2., (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2024
- Full Text
- View/download PDF
10. Enhanced efficacy of glycoengineered rice cell-produced trastuzumab.
- Author
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Shin JH, Oh S, Jang MH, Lee SY, Min C, Eu YJ, Begum H, Kim JC, Lee GR, Oh HB, Paul MJ, Ma JK, Gwak HS, Youn H, and Kim SR
- Subjects
- Humans, Animals, Cell Line, Tumor, Glycosylation, Mice, Female, Cell Proliferation drug effects, Oryza genetics, Oryza metabolism, Trastuzumab, Plants, Genetically Modified metabolism
- Abstract
For several decades, a plant-based expression system has been proposed as an alternative platform for the production of biopharmaceuticals including therapeutic monoclonal antibodies (mAbs), but the immunogenicity concerns associated with plant-specific N-glycans attached in plant-based biopharmaceuticals has not been completely solved. To eliminate all plant-specific N-glycan structure, eight genes involved in plant-specific N-glycosylation were mutated in rice (Oryza sativa) using the CRISPR/Cas9 system. The glycoengineered cell lines, PhytoRice®, contained a predominant GnGn (G0) glycoform. The gene for codon-optimized trastuzumab (TMab) was then introduced into PhytoRice® through Agrobacterium co-cultivation. Selected cell lines were suspension cultured, and TMab secreted from cells was purified from the cultured media. The amino acid sequence of the TMab produced by PhytoRice® (P-TMab) was identical to that of TMab. The inhibitory effect of P-TMab on the proliferation of the BT-474 cancer cell line was significantly enhanced at concentrations above 1 μg/mL (****P < 0.0001). P-TMab bound to a FcγRIIIa variant, FcγRIIIa-F158, more than 2.7 times more effectively than TMab. The ADCC efficacy of P-TMab against Jurkat cells was 2.6 times higher than that of TMab in an in vitro ADCC assay. Furthermore, P-TMab demonstrated efficient tumour uptake with less liver uptake compared to TMab in a xenograft assay using the BT-474 mouse model. These results suggest that the glycoengineered PhytoRice® could be an alternative platform for mAb production compared to current CHO cells, and P-TMab has a novel and enhanced efficacy compared to TMab., (© 2024 The Author(s). Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.)
- Published
- 2024
- Full Text
- View/download PDF
11. Identification of the Novel HLA-DQB1*06:432 Allele by Sequence-Based Typing.
- Author
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Yang JJ and Oh HB
- Subjects
- Humans, Amino Acid Substitution, Sequence Alignment, HLA-DQ beta-Chains genetics, Alleles, Histocompatibility Testing, Sequence Analysis, DNA methods, Exons, Codon, Base Sequence
- Abstract
HLA-DQB1*06:432 differs from HLA-DQB1*06:09:01:01 by a nonsynonymous nucleotide substitution in codon 186, changing Valine to Methionine., (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2024
- Full Text
- View/download PDF
12. The HLA-C*14:152 Allele Was Identified Using a Next-Generation Sequencing Method.
- Author
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Kim SH, Yang JJ, and Oh HB
- Subjects
- Humans, Base Sequence, Sequence Analysis, DNA methods, Polymorphism, Single Nucleotide, Sequence Alignment, HLA-C Antigens genetics, Alleles, Exons, High-Throughput Nucleotide Sequencing methods, Histocompatibility Testing methods
- Abstract
HLA-C*14:152 differs from HLA-C*14:02:01:01 by a non-synonymous nucleotide substitution in exon 5., (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2024
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13. Robotic and Laparoscopic Adrenalectomy for Pheochromocytoma: An International Multicenter Study.
- Author
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Parente A, Verhoeff K, Wang Y, Wang N, Wang Z, Śledziński M, Hellmann A, Raffaelli M, Pennestrì F, Sywak M, Papachristos AJ, Palazzo FF, Sung TY, Kim BC, Lee YM, Eatock F, Anderson H, Iacobone M, Daukša A, Makay O, Turk Y, Basut Atalay H, Nieveen van Dijkum EJM, Engelsman AF, Holscher I, Materazzi G, Rossi L, Becucci C, Shore SL, Fung C, Waghorn A, Mihai R, Balasubramanian SP, Pannu A, Tatarano S, Velázquez-Fernández D, Miller JA, Serrao-Brown H, Chen Y, Demarchi MS, Djafarrian R, Doran H, Wang K, Stechman MJ, Perry H, Hubbard J, Lamas C, Mercer P, MacPherson J, Lumbiganon S, Calatayud M, Alexandra Hanzu F, Vidal O, Araujo-Castro M, Minguez Ojeda C, Papavramidis T, Rodríguez de Vera Gómez P, Aldrees A, Altwjry T, Valdés N, Álvarez-Escola C, García Sanz I, Blanco Carrera C, Manjón-Miguélez L, De Miguel Novoa P, Recasens M, García Centeno R, Robles Lázaro C, Van Den Heede K, Van Slycke S, Michalopoulou T, Aspinall S, Melvin R, Lau JWL, Cheah WK, Tang MH, Oh HB, Ayuk J, and Sutcliffe RP
- Abstract
Background and Objective: Robotic adrenalectomy (RA) has attracted interest as an alternative to laparoscopic adrenalectomy (LA) for patients with pheochromocytoma, although its beneficial effects are uncertain. Our aim was to compare RA and LA outcomes for these patients., Methods: Data for patients who underwent RA or LA for pheochromocytoma in 46 international centers between 2012 and 2022 were reviewed. We analyzed baseline characteristics and postoperative complications at discharge, 90 d, and 1 yr. We conducted propensity score matching (PSM; 1:1 ratio) and multivariable analyses to evaluate outcomes and risk factors for the occurrence of complications and higher Comprehensive Complication Index (CCI)., Key Findings and Limitations: Of 1755 patients, 1613 (91.9%) underwent LA and 142 (8.1%) underwent RA. Estimated blood loss, conversion rate, complication rate, and CCI at discharge, 90 d, and 1 yr were similar between the groups. However, RA was associated with a longer operative time in comparison to LA (100 vs 123 min; p < 0.001), but not after PSM (p = 0.120). Multivariable analysis revealed that Charlson comorbidity index (odds ratio [OR] 1.17, 95% confidence interval [CI] 1.07-1.29; p = 0.001), and tumor size per 1-cm increment (OR 1.13, 95% CI 1.07-1.21; p < 0.001) were independently associated with the incidence of complications, but there was no significant difference in complication rates between the LA and RA groups (OR 1.09, 95% CI 0.63-1.87; p = 0.767). After PSM, RA was associated with a lower rate of severe (grade ≥3a) complications in comparison to LA (p = 0.023)., Conclusions and Clinical Implications: RA is a safe alternative to LA and yields similar outcomes for patients with pheochromocytoma. RA may be associated with a lower likelihood of severe complications. Further studies are warranted to determine the role of robotic surgery in pheochromocytoma., Patient Summary: Pheochromocytoma is a rare tumor in the adrenal gland and the gold-standard treatment is surgical removal. We assessed patient outcomes after robot-assisted surgery compared with laparoscopic surgery and found that outcomes are similar, but the rate of severe complications may be lower if a surgical robot is used., (Copyright © 2024 European Association of Urology. Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
14. Long-term isoagglutinin monitoring after ABO-incompatible kidney transplantation.
- Author
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Kim HJ, Chung Y, Kim H, Ko Y, Kim YH, Hwang SH, Oh HB, and Ko DH
- Abstract
Objectives: This study aimed to evaluate whether a 2-week period of daily isoagglutinin titer testing after ABO-incompatible kidney transplantation (ABOi-KT) is sufficient to ensure successful engraftment and to advocate for an extension of the monitoring duration in specific situations., Methods: We reviewed patients from January 2022 to December 2023 at Asan Medical Center who underwent therapeutic plasma exchange (TPE) due to elevated ABO antibody titers and suspected acute antibody-mediated rejection (AMR) after ABOi-KT. Data collected included pre- and posttransplantation laboratory results, clinical and procedural information, imaging studies, and needle biopsy results of the renal graft., Results: We encountered 3 cases of acute AMR 2 weeks after transplantation. All cases exhibited simultaneous increases in anti-ABO antibody isoagglutinin titers, creatinine, and C-reactive protein levels. Clinical signs, including fever, suggested possible infection, and renal graft biopsy, confirmed AMR in all cases. Two cases underwent graftectomy, while the third recovered renal function after conservative treatment, including TPE., Conclusions: Our findings suggest that a 2-week monitoring period for isoagglutinin titers after ABOi-KT may not be sufficient to detect late AMR. Extending the monitoring duration and considering lifelong fresh-frozen plasma transfusion with graft-compatible blood types, along with periodic isoagglutinin titer testing in cases of suspected AMR, may improve long-term graft outcomes., (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)
- Published
- 2024
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- View/download PDF
15. Assessment of biodegradation and toxicity of alternative plasticizer di(2-ethylhexyl) terephthalate: Impacts on microbial biofilms, metabolism, and reactive oxygen species-mediated stress response.
- Author
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Mohamed DFMS, Tarafdar A, Lee SY, Oh HB, and Kwon JH
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- Rhodococcus metabolism, Rhodococcus drug effects, Polyvinyl Chloride toxicity, Diethylhexyl Phthalate toxicity, Plasticizers toxicity, Biofilms drug effects, Reactive Oxygen Species metabolism, Phthalic Acids toxicity, Phthalic Acids metabolism, Biodegradation, Environmental
- Abstract
Although di(2-ethylhexyl) terephthalate (DOTP) is being widely adopted as a non-phthalate plasticizer, existing research primarily focuses on human and rat toxicity. This leaves a significant gap in our understanding of their impact on microbial communities. This study assessed the biodegradation and toxicity of DOTP on microbes, focusing on its impact on biofilms and microbial metabolism using Rhodococcus ruber as a representative bacterial strain. DOTP is commonly found in mass fractions between 0.6 and 20% v/v in various soft plastic products. This study used polyvinyl chloride films (PVC) with varying DOTP concentrations (range 1-10% v/v) as a surface for analysis of biofilm growth. Cell viability and bacterial stress responses were tested using LIVE/DEAD™ BacLight™ Bacterial Viability Kit and by the detection of reactive oxygen species using CellROX™ Green Reagent, respectively. An increase in the volume of dead cells (in the plastisphere biofilm) was observed with increasing DOTP concentrations in experiments using PVC films, indicating the potential negative impact of DOTP on microbial communities. Even at a relatively low concentration of DOTP (1%), signs of stress in the microbes were noticed, while concentrations above 5% compromised their ability to survive. This research provides a new understanding of the environmental impacts of alternative plasticizers, prompting the need for additional research into their wider effects on both the environment and human health., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Jung-Hwan Kwon reports financial support was provided by National Research Foundation of Korea., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
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16. Genomic full-length sequence of the novel HLA-B*40:540N allele.
- Author
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Yang JJ and Oh HB
- Subjects
- Humans, Codon, Histocompatibility Testing, HLA-B Antigens genetics, HLA-B40 Antigen genetics, Sequence Alignment, Sequence Analysis, DNA, Tissue Donors, Male, Middle Aged, Alleles, Base Sequence, Exons
- Abstract
The coding sequence of HLA-B*40:540N differs from HLA-B*40:02:01:01 by a non-synonymous nucleotide substitution in exon 3., (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2024
- Full Text
- View/download PDF
17. The novel HLA-DQA1*03:75 allele identified in a Korean kidney donor.
- Author
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Kim SH, Yang JJ, and Oh HB
- Subjects
- Humans, Republic of Korea, Asian People genetics, Sequence Analysis, DNA methods, Base Sequence, HLA-DQ alpha-Chains genetics, Alleles, Exons, Kidney Transplantation, Histocompatibility Testing, Tissue Donors
- Abstract
HLA-DQA1*03:75 differs from HLA-DQA1*03:02:01:01 by a single non-synonymous nucleotide substitution in exon 2., (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2024
- Full Text
- View/download PDF
18. Genomic full-length sequence of the novel HLA-C*06:364 allele.
- Author
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Yang JJ and Oh HB
- Subjects
- Humans, Histocompatibility Testing, Base Sequence, Sequence Analysis, DNA methods, Codon, Sequence Alignment, HLA-C Antigens genetics, Alleles, Exons
- Abstract
HLA-C*06:364 differs from HLA-C*06:02:01:01 by a non-synonymous nucleotide substitution in exon 3., (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2024
- Full Text
- View/download PDF
19. Thermodynamic Reversal and Structural Correlation of 24-Crown-8/Protonated Tryptophan and 24-Crown 8/Protonated Serine Noncovalent Complexes in the Gas Phase vs in Solution: Quantum Chemical Analysis.
- Author
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Oh YH, Lee SY, Kong X, Oh HB, and Lee S
- Abstract
We investigate the structures of 24-crown-8/H
+ /l-tryptophan (CR/TrpH+ ) and 24-crown-8/H+ /l-serine (CR/SerH+ ) noncovalent host-guest complex both in the gas phase and in an aqueous solution by quantum chemical methods. The Gibbs free energies of the complex in the two phases are calculated to determine the thermodynamically most favorable conformer in each phase. Our predictions indicate that both the carboxyl and the ammonium in CR/TrpH+ and the ammonium in the CR/SerH+ complexes in the lowest Gibbs free energy configurations form hydrogen bonds (H-bonds) with the CR host in the gas phase, while the conformer with the "naked" (devoid of H-bond with the CR host) -CO2 H (and/or -OH) is much less favorable (Gibbs free energy higher by >3.6 kcal/mol). In the solution phase, however, a "thermodynamic reversal" occurs, making the higher Gibbs free energy gas-phase CR/TrpH+ and CR/SerH+ conformers thermodynamically more favorable under the influence of solvent molecules. Consequently, the global minimum Gibbs free energy structure in solution is structurally correlated with the thermodynamically much less gas-phase conformer. Discussions are provided concerning the possibility of elucidating host-guest-solvent interactions in solution from the gas-phase host-guest configurations in molecular detail., Competing Interests: The authors declare no competing financial interest., (© 2024 The Authors. Published by American Chemical Society.)- Published
- 2024
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- View/download PDF
20. Trends in category and grade for therapeutic plasma exchange in the latest guideline on therapeutic apheresis by the American Society for Apheresis: Hurdles in pursuing evidence-based medicine.
- Author
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Kim HJ, Chung Y, Kim H, Hwang SH, Oh HB, and Ko DH
- Subjects
- Humans, Practice Guidelines as Topic, Societies, Medical, United States, Female, Male, Plasma Exchange methods, Evidence-Based Medicine, Blood Component Removal methods
- Abstract
Background and Objectives: The Writing Committee of American Society for Apheresis released the ninth edition of guidelines for therapeutic apheresis in 2023. Categories have been a part of the guidelines since the first edition, and the grading system was introduced in the fifth edition, with updates in every new edition. In this study, we investigated the category and grade change trends through the latest five editions, focusing on therapeutic plasma exchange, to suggest future directions as part of evidence-based medicine., Materials and Methods: Categories and grades for therapeutic plasma exchange (TPE) were collected and analysed from the fifth through ninth editions. We aligned classification changes to the ninth edition's clinical context and compared its categories and grades with those introduced in the guideline., Results: Among 166 total indications in the ninth edition, 118 included TPE procedure, either as a sole treatment or as one of the therapeutic apheresis techniques. The total number of indications changed, but Category III remained predominant throughout the editions. Similarly, Grade 2C consistently emerged as the most prevalent grade. Notably, 24 cases had grade changes. Of the 16 cases with evidence quality changes, the quality weakened in six and improved in 10. Evidence levels were not improved throughout the study period for 102 clinical conditions., Conclusion: To address gaps in evidence quality, international collaboration is imperative to establish comprehensive large-scale studies or randomized controlled trials. This will refine the use of therapeutic apheresis, including TPE, to foster evidence-based advancements in clinical practice., (© 2024 International Society of Blood Transfusion.)
- Published
- 2024
- Full Text
- View/download PDF
21. HLA-DRB1 is associated with cefaclor-induced immediate hypersensitivity.
- Author
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Park SY, Park SY, Seo S, Kwon HS, Kim SH, Kim SH, Park HK, Chang YS, Kim CW, Lee BJ, Park HS, Cho YS, Oh HB, Ostrov DA, Won S, and Kim TB
- Abstract
Background: Drug-induced hypersensitivity such as anaphylaxis is an important cause of drug-related morbidity and mortality. Cefaclor is a leading cause of drug induced type I hypersensitivity in Korea, but little is yet known about genetic biomarkers to predict this hypersensitivity reaction. We aimed to evaluate the possible involvement of genes in cefaclor induced type I hypersensitivity., Methods: Whole exome sequencing (WES) and HLA genotyping were performed in 43 patients with cefaclor induced type I hypersensitivity. In addition, homology modeling was performed to identify the binding forms of cefaclor to HLA site., Results: Anaphylaxis was the most common phenotype of cefaclor hypersensitivity (90.69%). WES results show that rs62242177 and rs62242178 located in LIMD1 region were genome-wide significant at the 5 × 10
-8 significance level. Cefaclor induced type I hypersensitivity was significantly associated with HLA-DRB1∗04:03 (OR 4.61 [95% CI 1.51-14.09], P < 0.002) and HLA-DRB1∗14:54 (OR 3.86 [95% CI 1.09-13.67], P < 0.002)., Conclusion: LIMD1, HLA-DRB1∗04:03 and HLA-DRB1∗14:54 may affect susceptibility to cefaclor induced type I hypersensitivity. Further confirmative studies with a larger patient population should be performed to ascertain the role of HLA-DRB1 and LIMD1 in the development of cefaclor induced hypersensitivity., Competing Interests: The authors have no conflict of interest relevant to this article to disclose., (© 2024 The Authors.)- Published
- 2024
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22. Linker-Preserved Iron Metal-Organic Framework-Based Lateral Flow Assay for Sensitive Transglutaminase 2 Detection in Urine Through Machine Learning-Assisted Colorimetric Analysis.
- Author
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Supianto M, Yoo DK, Hwang H, Oh HB, Jhung SH, and Lee HJ
- Subjects
- Humans, Colorimetry methods, Iron, Reproducibility of Results, Metal-Organic Frameworks, Protein Glutamine gamma Glutamyltransferase 2 urine, Renal Insufficiency, Chronic diagnosis, Renal Insufficiency, Chronic urine, Machine Learning
- Abstract
A groundbreaking demonstration of the utilization of the metal-organic framework MIL-101(Fe) as an exceptionally perceptive visual label in colorimetric lateral flow assays (LFA) is described. This pioneering approach enables the precise identification of transglutaminase 2 (TGM2), a recognized biomarker for chronic kidney disease (CKD), in urine specimens, which offers a remarkably sensitive naked-eye detection mechanism. The surface of MIL-101(Fe) was modified with oxalyl chloride, adipoyl chloride, and poly(acrylic) acid (PAA); these not only improved the labeling material stability in a complex matrix but also achieved a systematic control in the detection limit of the TGM2 concentration using our LFA platform. The advanced LFA with the MIL-101(Fe)-PAA label can detect TGM2 concentrations down to 0.012, 0.009, and 0.010 nM in Tris-HCl buffer, urine, and desalted urine, respectively, which are approximately 55-fold lower than those for a conventional AuNP-based LFAs. Aside from rapid TGM2 detection (i.e., within 20 min), the performance of the MIL-101(Fe)-PAA-based LFA on reproducibility [coefficients of variation (CV) < 2.9%] and recovery (95.9-103.2%) along with storage stability within 25 days of observation (CV < 6.0%) shows an acceptable parameter range for quantitative analysis. A sophisticated sensing method grounded in machine learning principles was also developed, specifically aimed at precisely deducing the TGM2 concentration by analyzing immunoreaction sites. More importantly, our developed LFA offers potential for clinical measurement of TGM2 concentration in normal human urine and CKD patients' samples.
- Published
- 2024
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23. Complementary hydrophobic interaction of the redox enzyme maturation protein NarJ with the signal peptide of the respiratory nitrate reductase NarG.
- Author
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Song WS, Kim JH, Namgung B, Cho HY, Shin H, Oh HB, Ha NC, and Yoon SI
- Subjects
- Nitrate Reductase chemistry, Nitrate Reductase metabolism, Oxidation-Reduction, Hydrophobic and Hydrophilic Interactions, Protein Sorting Signals, Escherichia coli metabolism
- Abstract
In bacteria, NarJ plays an essential role as a redox enzyme maturation protein in the assembly of the nitrate reductase NarGHI by interacting with the N-terminal signal peptide of NarG to facilitate cofactor incorporation into NarG. The purpose of our research was to elucidate the exact mechanism of NarG signal peptide recognition by NarJ. We determined the structures of NarJ alone and in complex with the signal peptide of NarG via X-ray crystallography and verified the NarJ-NarG interaction through mutational, binding, and molecular dynamics simulation studies. NarJ adopts a curved α-helix bundle structure with a U-shaped hydrophobic groove on its concave side. This groove accommodates the signal peptide of NarG via a dual binding mode in which the left and right parts of the NarJ groove each interact with two consecutive hydrophobic residues from the N- and C-terminal regions of the NarG signal peptide, respectively, through shape and chemical complementarity. This binding is accompanied by unwinding of the helical structure of the NarG signal peptide and by stabilization of the NarG-binding loop of NarJ. We conclude that NarJ recognizes the NarG signal peptide through a complementary hydrophobic interaction mechanism that mediates a structural rearrangement., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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24. Genomic full-length sequence of the novel HLA-A*02:1100 allele.
- Author
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Yang JJ and Oh HB
- Subjects
- Humans, Base Sequence, Alleles, Sequence Analysis, DNA, Genomics, HLA-A Antigens genetics
- Abstract
HLA-A*02:1100 differs from HLA-A*02:01:01:01 by a single non-synonymous nucleotide substitution in codon 76 of exon 2., (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2024
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25. Structural Identification of Ginsenoside Based on UPLC-QTOF-MS of Black Ginseng ( Panax Ginseng C.A. Mayer).
- Author
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Oh HB, Jeong DE, Lee DE, Yoo JH, Kim YS, and Kim TY
- Abstract
Black ginseng (BG) is processed ginseng traditionally made in Korea via the steaming and drying of ginseng root through three or more cycles, leading to changes in its appearance due to the Maillard reaction on its surface, resulting in a dark coloration. In this study, we explored markers for differentiating processed ginseng by analyzing the chemical characteristics of BG. We elucidated a new method for the structural identification of ginsenoside metabolites and described the features of processed ginseng using UPLC-QTOF-MS in the positive ion mode. We confirmed that maltose, glucose, and fructose, along with L-arginine, L-histidine, and L-lysine, were the key compounds responsible for the changes in the external quality of BG. These compounds can serve as important metabolic markers for distinguishing BG from conventionally processed ginseng. The major characteristics of white ginseng, red ginseng, and BG can be distinguished based on their high-polarity and low-polarity ginsenosides, and a precise method for the structural elucidation of ginsenosides in the positive ion mode is presented.
- Published
- 2024
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26. Atypical formations of gintonin lysophosphatidic acids as new materials and their beneficial effects on degenerative diseases.
- Author
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Kim JH, Lee RM, Oh HB, Kim TY, Rhim H, Choi YK, Kim JH, Oh S, Kim DG, Cho IH, and Nah SY
- Abstract
Fresh ginseng is prone to spoilage due to its high moisture content. For long-term storage, most fresh ginsengs are dried to white ginseng (WG) or steamed for hours at high temperature/pressure and dried to form Korean Red ginseng (KRG). They are further processed for ginseng products when subjected to hot water extraction/concentration under pressure. These WG or KRG preparation processes affect ginsenoside compositions and also other ginseng components, probably during treatments like steaming and drying, to form diverse bioactive phospholipids. It is known that ginseng contains high amounts of gintonin lysophosphatidic acids (LPAs). LPAs are simple lipid-derived growth factors in animals and humans and act as exogenous ligands of six GTP-binding-protein coupled LPA receptor subtypes. LPAs play diverse roles ranging from brain development to hair growth in animals and humans. LPA-mediated signaling pathways involve various GTP-binding proteins to regulate downstream pathways like [Ca
2+ ]i transient induction. Recent studies have shown that gintonin exhibits anti-Alzheimer's disease and anti-arthritis effects in vitro and in vivo mediated by gintonin LPAs, the active ingredients of gintonin, a ginseng-derived neurotrophin. However, little is known about how gintonin LPAs are formed in high amounts in ginseng compared to other herbs. This review introduces atypical or non-enzymatic pathways under the conversion of ginseng phospholipids into gintonin LPAs during steaming and extraction/concentration processes, which exert beneficial effects against degenerative diseases, including Alzheimer's disease and arthritis in animals and humans via LPA receptors., (© 2023 The Korean Society of Ginseng. Publishing services by Elsevier B.V.)- Published
- 2024
- Full Text
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27. The novel HLA-C allele, HLA-C*01:02:84 allele, identified in a solid organ transplantation donor.
- Author
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Yang JH, Yang JJ, and Oh HB
- Subjects
- Humans, Alleles, Sequence Analysis, DNA, Genes, MHC Class I, HLA-C Antigens genetics, Organ Transplantation
- Abstract
HLA-C*01:02:84 differs from HLA-C*01:02:01:01 by one synonymous nucleotide substitution in codon 48., (© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2024
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- View/download PDF
28. The HLA-B*58:01:43 allele identified in a Korean individual awaiting hematopoietic stem cell transplant.
- Author
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Yang JJ and Oh HB
- Subjects
- Humans, Base Sequence, Alleles, Histocompatibility Testing, Republic of Korea, Sequence Analysis, DNA, HLA-B Antigens genetics, Hematopoietic Stem Cell Transplantation
- Abstract
HLA-B*58:01:43 differs from HLA-B*58:01:01:01 by one synonymous nucleotide substitution in codon 197., (© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2024
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- View/download PDF
29. Genomic full-length sequence of the novel HLA-DQB1*04:01:01:04 allele.
- Author
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Yang JJ and Oh HB
- Subjects
- Humans, Exons genetics, Alleles, HLA-DQ beta-Chains genetics, Genomics
- Abstract
The sequence of HLA-DQB1*04:01:01:04 differs from HLA-DQB1*04:01:01:03 by four nucleotide deletion in intron 2., (© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2024
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30. Discovery of the HLA-DQB1*06:465 allele, a variant of HLA-DQB1*06:04:01:01.
- Author
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Yang JJ and Oh HB
- Subjects
- Humans, Exons genetics, Alleles, HLA-DQ beta-Chains genetics, Base Sequence
- Abstract
HLA-DQB1*06:465 differs from HLA-DQB1*06:04:01:01 by a non-synonymous nucleotide substitution in codon 38., (© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2024
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31. Clinical significances and distribution of unexpected antibodies found in infants.
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Kim HJ, Seo SW, Chung Y, Kim H, Hwang SH, Oh HB, and Ko DH
- Subjects
- Humans, Infant, Infant, Newborn, Clinical Relevance, Isoantibodies, Retrospective Studies, Blood Group Antigens, Erythroblastosis, Fetal
- Abstract
Introduction: The unexpected antibody test is an essential for ensuring the safety of blood transfusions. In infants, different pre-transfusion tests and transfusion strategies are needed due to their immature antigen/antibody system. This study aims to analyze the pattern of unexpected antibodies and their clinical significance in infants., Methods: A retrospective analysis was conducted on the results of unexpected antibody identification tests performed on infants under one year of age at Asan Medical Center from 1999 to 2022. Patients' unexpected antibody identification test results and clinical information were investigated. The results of unexpected antibody identification and phenotype of each patient's mother were collected., Results: 45 cases of antibody results were studied. 25 cases were found in infants under 4 months of age, and 18 cases (76%) were associated with hemolytic disease of the fetus and newborn (HDFN). The most common unexpected antibody in infants was anti-M (17 cases). There was one case of severe HDFN caused by anti-M. In 10 cases, anti-E and anti-c were found together, and 9 of these cases were associated with HDFN. There were four cases with a history of previous transfusion., Conclusions: Non-ABO antibodies found in infants showed a different pattern compared to adults. Interpreting unexpected antibody tests in infants, it is important to consider the clinical status of the infant and the test results of the mother, due to possibility of HDFN. To our knowledge, this is the first study to reveal the distribution and clinical significances of unexpected antibodies found in infants in Korea., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Published
- 2023
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32. Hexameric structure of the flagellar master regulator FlhDC from Cupriavidus necator and its interaction with flagellar promoter DNA.
- Author
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Cho SY, Oh HB, and Yoon SI
- Subjects
- Trans-Activators metabolism, Bacterial Proteins metabolism, Promoter Regions, Genetic, Escherichia coli metabolism, DNA metabolism, Flagella metabolism, Gene Expression Regulation, Bacterial, Cupriavidus necator genetics, Cupriavidus necator metabolism, Escherichia coli Proteins metabolism
- Abstract
Bacterial flagella are assembled with ∼30 different proteins in a defined order via diverse regulatory systems. In gram-negative bacteria from the Gammaproteobacteria and Betaproteobacteria classes, the transcription of flagellar genes is strictly controlled by the master regulator FlhDC. In Gammaproteobacteria species, the FlhDC complex has been shown to activate flagellar expression by directly interacting with the promoter region in flagellar genes. To obtain the DNA-binding mechanism of FlhDC and determine the conserved and distinct structural features of Betaproteobacteria and Gammaproteobacteria FlhDCs that are necessary for their functions, we determined the crystal structure of Betaproteobacteria Cupriavidus necator FlhDC (cnFlhDC) and biochemically analyzed its DNA-binding capacity. cnFlhDC specifically recognized the promoter DNA of the class II flagellar genes flgB and flhB. cnFlhDC adopts a ring-like heterohexameric structure (cnFlhD
4 C2 ) and harbors two Zn-Cys clusters, as observed for Gammaproteobacteria Escherichia coli FlhDC (ecFlhDC). The cnFlhDC structure exhibits positively charged surfaces across two FlhDC subunits as a putative DNA-binding site. Noticeably, the positive patch of cnFlhDC is continuous, in contrast to the separated patches of ecFlhDC. Moreover, the ternary intersection of cnFlhD4 C2 behind the Zn-Cys cluster forms a unique protruding neutral structure, which is replaced with a charged cavity in the ecFlhDC structure., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)- Published
- 2023
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33. Machine learning liquid chromatography retention time prediction model augments the dansylation strategy for metabolite analysis of urine samples.
- Author
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Choi E, Yoo WJ, Jang HY, Kim TY, Lee SK, and Oh HB
- Subjects
- Humans, Female, Dansyl Compounds chemistry, Chromatography, Liquid methods, Mass Spectrometry methods, Amines urine, Phenols, Isotope Labeling, Metabolome, Metabolomics methods
- Abstract
Herein, a standalone software equipped with a graphic user interface (GUI) is developed to predict liquid chromatography mass spectrometry (LC-MS) retention times (RTs) of dansylated metabolites. Dansylation metabolomics strategy developed by Li et al. narrows down a vast chemical space of metabolites into the metabolites containing amines and phenolic hydroxyls. Combined with differential isotope labeling, e.g.,
12 C-reagent labeled individual samples spiked with a13 C-reagent labeled reference or pooled sample, LC-MS analysis of the dansylated samples enables accurate relative quantification of all labeled metabolites. Herein, the LC-RTs for dansylated metabolites are predicted using an artificial neural network (ANN) machine-learning model. For the ANN modeling, 315 dansylated urine metabolites obtained from the DnsID database are used. The ANN LC-RT prediction model was reliable, with a mean absolute deviation of 0.74 min for the 30 min LC run. In the RT model, a deviation of more than 2 min was observed in only 3.2% of the total 315 metabolites, while a deviation of 1.5 min or more was observed in 11% of the metabolites. Furthermore, it was found that the LC-RT prediction was also reliable even for metabolites containing both amine and phenolic functional groups that can undergo dansylation on either one of the two functional groups, resulting in the generation of two isomeric forms. This RT-prediction model is embedded into a user-friendly GUI and can be used for identifying nontargeted dansylated metabolites with unknown RTs, along with accurate mass measurements. Furthermore, it is demonstrated that the developed software can help identify metabolites from a urine sample of an anonymous healthy pregnant woman., Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest. The funder had no role in the design of the study; in the collection, analyzes, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2023
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- View/download PDF
34. Structural and Biochemical Analysis of the Recombination Mediator Protein RecR from Campylobacter jejuni .
- Author
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Lee SJ, Ahn SY, Oh HB, Kim SY, Song WS, and Yoon SI
- Subjects
- Cell Nucleus, Cognition, Cysteine, DNA Damage, Campylobacter jejuni genetics
- Abstract
The recombination mediator complex RecFOR, consisting of the RecF, RecO, and RecR proteins, is needed to initiate homologous recombination in bacteria by positioning the recombinase protein RecA on damaged DNA. Bacteria from the phylum Campylobacterota, such as the pathogen Campylobacter jejuni , lack the recF gene and trigger homologous recombination using only RecR and RecO. To elucidate the functional properties of C. jejuni RecR (cjRecR) in recombination initiation that differ from or are similar to those in RecF-expressing bacteria, we determined the crystal structure of cjRecR and performed structure-based binding analyses. cjRecR forms a rectangular ring-like tetrameric structure and coordinates a zinc ion using four cysteine residues, as observed for RecR proteins from RecF-expressing bacteria. However, the loop of RecR that has been shown to recognize RecO and RecF in RecF-expressing bacteria is substantially shorter in cjRecR as a canonical feature of Campylobacterota RecR proteins, indicating that cjRecR lost a part of the loop in evolution due to the lack of RecF and has a low RecO-binding affinity. Furthermore, cjRecR features a larger positive patch and exhibits substantially higher ssDNA-binding affinity than RecR from RecF-expressing bacteria. Our study provides a framework for a deeper understanding of the RecOR-mediated recombination pathway.
- Published
- 2023
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- View/download PDF
35. Identification of the novel HLA-B*58:138 allele in a Korean individual.
- Author
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Youk HJ, Kwon OJ, and Oh HB
- Subjects
- Humans, Alleles, Codon, Republic of Korea, Sequence Analysis, DNA, HLA-B Antigens genetics, Genes, MHC Class I
- Abstract
HLA-B*58:138 differs from HLA-B*58:01:01:01 by one nucleotide substitution in exon 3 at codon 145., (© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2023
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- View/download PDF
36. Identification of the novel HLA-A*11:423 allele by sequencing-based typing.
- Author
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Yang JJ and Oh HB
- Subjects
- Humans, Alleles, Codon, Sequence Analysis, DNA, Nucleotides, HLA-A Antigens genetics
- Abstract
HLA-A*11:423 differs from HLA-A*11:01:01:01 by a non-synonymous nucleotide substitution in codon 170, changing Arginine to Lysine., (© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2023
- Full Text
- View/download PDF
37. Investigation of variables affecting the immunogenicity of blood group antigens using a calculation formula.
- Author
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Chung Y, Kim HJ, Kim H, Hwang SH, Oh HB, and Ko DH
- Subjects
- Blood Transfusion, Antibodies, Homozygote, Blood Group Antigens
- Abstract
Previous studies on the immunogenicity of blood group antigens have utilized a formula incorporating antigen frequencies and relative frequencies of unexpected antibodies to the corresponding antigens. This study was aimed at investigating other variables potentially affecting the estimation of immunogenicity using this formula. We examined the effect of multiple transfusions, as there are more chance for a recipient to receive repeated transfusions rather than only once; the effect of antigen density, which may vary depending on homozygote/heterozygote; and the effect of unreliability of the observed frequency of rare antibodies and antigens. For multiple transfusions, the expected antibody frequency increased as the number of transfusions increased. For antigen density, the immunogenicity was falsely low for the low-prevalence antigen, and this tendency intensified as the effect of antigen density increased. Expected antibody frequencies were significantly affected by the uncertainties caused by estimation of small numbers. This study showed that the effects of various factors on the immunogenicity of blood group antigens depended on the antigen frequency. Estimating the immunogenicity of blood group antigens requires acknowledging the diverse factors that can affect it and interpreting the findings with caution., (© 2023. The Author(s).)
- Published
- 2023
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38. Transfusion thresholds: the need for a patient-centered approach in hematologic disorders that require chronic transfusion therapy.
- Author
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Kim HJ, Hwang SH, Oh HB, and Ko DH
- Abstract
Transfusion is an essential life-sustaining treatment for many patients. However, unnecessary transfusion has been reported to be related to worse patient outcomes. Further, owing to the recent pandemic, blood supply has been more challenging to maintain. Many studies have been conducted to elucidate the optimal transfusion threshold for many clinical conditions, and most suggested that a restrictive transfusion strategy has advantages over a liberal transfusion strategy. Hematologic disorders, which require chronic transfusion in many cases, have not been the main subjects of such studies, and only little evidence is available regarding the optimal transfusion threshold in these patients. According to several recent studies, a liberal transfusion strategy is preferable for patients with hematologic disorders due to their quality of life. A patient-centered approach is needed for proper management of hematologic disorders.
- Published
- 2023
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39. Relative immunogenicity of blood group antigens: First report in a Korean population.
- Author
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Chung Y, Kim JS, Youk HJ, Kim H, Hwang SH, Oh HB, and Ko DH
- Subjects
- Humans, Male, Isoantibodies, Blood Transfusion, Asian People, Republic of Korea, Erythrocytes, Blood Group Antigens
- Abstract
Background: The immunogenicity of a blood group antigen is a measure of its likelihood of inducing alloantibodies. Although the immunogenicity of blood group antigens has been analyzed in Caucasian populations, immunogenicity to date has not been analyzed in Asian subjects. The present study therefore evaluated the relative immunogenicity of blood group antigens in a Korean population., Study Design and Methods: All available data of unexpected antibody identification tests performed at Asan Medical Center between 1997 and 2021 were analyzed. The relative immunogenicity of a blood group antigen relative to K antigen was calculated based on relative numbers of alloantibodies and the probabilities of antigen-negative recipients receiving antigen-positive RBC units., Results: A total of 3898 antibody identification results were included, with 1632 (41.9 %) from male patients. The ranking of antigen immunogenicity was: E > c > e > C > K > Jk(a) > Lu(a) > S > Fy(a) > Fy(b) > Jk(b) > Di(b) > Di(a) in the total population and E > c > e > C > Jk(a) > Fy(a) > Fy(b) > S > K > Lu(a) > Jk(b) > Di(b) > Di(a) in male patients., Discussion: The rank order of immunogenicity for blood group antigens in this study provides information about relative immunogenecity in Koreans. These findings also provide supporting evidence regarding antigen selection for extended antigen-matched transfusions in recipients of multiple transfusions., Competing Interests: Conflict of interests None., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2023
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- View/download PDF
40. Author Correction: New free radical-initiated peptide sequencing (FRIPS) mass spectrometry reagent with high conjugation efficiency enabling single-step peptide sequencing.
- Author
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Lee ST, Park H, Jang I, Lee CS, Moon B, and Oh HB
- Published
- 2023
- Full Text
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41. Influence of mobile phase composition on the analytical sensitivity of LC-ESI-MS/MS for the concurrent analysis of bisphenols, parabens, chlorophenols, benzophenones, and alkylphenols.
- Author
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Lee KM, Han SM, Lee HJ, Kang M, Jeong TY, Son J, Min H, Cha S, Oh HB, Oh WK, and Lee J
- Subjects
- Humans, Tandem Mass Spectrometry methods, Parabens analysis, Benzophenones analysis, Chromatography, Liquid methods, Phenols urine, Spectrometry, Mass, Electrospray Ionization methods, Chlorophenols
- Abstract
Phenols are significant environmental endocrine disruptors that can have adverse health effects on exposed individuals. Correlating phenol exposure to potential health implications requires the development of a comprehensive and sensitive analytical method capable of analyzing multiple phenols in a single sample preparation and analytical run. Currently, no such method is available for multiple classes of phenols due to electrospray ionization (ESI) limitations in concurrent ionization and lack of sensitivity to certain phenols, particularly alkylphenols. In this study, we investigated the influence of mobile phase compositions in ESI on concurrent ionization and analytical sensitivity of liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) during the analysis of multiple classes of phenols, and we propose a comprehensive and sensitive analytical method for various classes of phenols (i.e., bisphenols, parabens, benzophenones, chlorophenols, and alkylphenols). The proposed method was affected by 0.5 mM ammonium fluoride under methanol conditions. It enabled the concurrent ionization of all the phenols and significantly improved the analytical sensitivity for bisphenols and alkylphenols, which typically have poor ionization efficiency. This method, combined with a "dilute and shoot" approach, allowed us to simultaneously quantify 38 phenols with good chromatographic behavior and sensitivity. Furthermore, the method was successfully applied to the analysis of 61 urine samples collected from aquatic (swimming) and land (indoor volleyball and outdoor football) athletes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
- Full Text
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42. Clinical Significance of Hemagglutination Grades Determined Using the IH-1000 Automated Blood Typing Instrument: Real-world Data.
- Author
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Park B, Kim JS, Youk HJ, Chung Y, Kim H, Hwang SH, Oh HB, and Ko DH
- Subjects
- Humans, Blood Grouping and Crossmatching, Hemagglutination
- Published
- 2022
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- View/download PDF
43. Structural basis of flagellar motility regulation by the MogR repressor and the GmaR antirepressor in Listeria monocytogenes.
- Author
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Cho SY, Na HW, Oh HB, Kwak YM, Song WS, Park SC, Jeon WJ, Cho H, Oh BC, Park J, Kang SG, Lee GS, and Yoon SI
- Subjects
- Humans, Bacterial Proteins metabolism, Flagella genetics, Flagella metabolism, Gene Expression Regulation, Bacterial, Listeria monocytogenes genetics
- Abstract
The pathogenic Listeria monocytogenes bacterium produces the flagellum as a locomotive organelle at or below 30°C outside the host, but it halts flagellar expression at 37°C inside the human host to evade the flagellum-induced immune response. Listeria monocytogenes GmaR is a thermosensor protein that coordinates flagellar expression by binding the master transcriptional repressor of flagellar genes (MogR) in a temperature-responsive manner. To understand the regulatory mechanism whereby GmaR exerts the antirepression activity on flagellar expression, we performed structural and mutational analyses of the GmaR-MogR system. At or below 30°C, GmaR exists as a functional monomer and forms a circularly enclosed multidomain structure via an interdomain interaction. GmaR in this conformation recognizes MogR using the C-terminal antirepressor domain in a unique dual binding mode and mediates the antirepressor function through direct competition and spatial restraint mechanisms. Surprisingly, at 37°C, GmaR rapidly forms autologous aggregates that are deficient in MogR neutralization capabilities., (© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Published
- 2022
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44. Detection of anti-B antibodies in a patient with A1B blood group: A case report and literature review.
- Author
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Youk HJ, Kim JS, Kim H, Hwang SH, Oh HB, Chung Y, and Ko DH
- Subjects
- Male, Humans, Middle Aged, Isoantibodies, Blood Grouping and Crossmatching, Antibodies, Anti-Idiotypic, ABO Blood-Group System, Lymphoma, Large B-Cell, Diffuse drug therapy
- Abstract
ABO antibodies occur naturally and usually exist as alloantibodies. They are the most clinically significant in cases of transfusions. However, there are very few reports on auto-anti-A or B. A 58-year-old man visited our hospital for evaluation of an inguinal mass. Blood typing was performed, while preparing the patient for an excisional biopsy. Forward and reverse typing showed a typical AB and A pattern. Results of the direct antiglobulin and unexpected antibody screening tests were negative. The serum did not react with AB3 cells. The biopsy revealed a diffuse large B-cell lymphoma. After completing four cycles of R-CHOP chemotherapy, the patient achieved complete remission. There were no anti-B antibodies found on repeat ABO typing. This report shares our experience on unexpected anti-B antibody findings in a patient with an A1B blood type. To the best of our knowledge, this is the first report of anti-B antibodies in a patient with an A1B blood type in Korea., Competing Interests: Conflict of interests All authors declare that there are no conflicts of interest relevant to this manuscript submitted to the journal., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
45. Nanoparticle-Based Visual Detection of Amplified DNA for Diagnosis of Hepatitis C Virus.
- Author
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Kim SK, Oh YH, Ko DH, Sung H, Oh HB, and Hwang SH
- Subjects
- Biotin, DNA, Digoxigenin, Hepacivirus genetics, Humans, Polystyrenes, Real-Time Polymerase Chain Reaction methods, Sensitivity and Specificity, Streptavidin, Hepatitis C diagnosis, Nanoparticles
- Abstract
Rapid, simple, and inexpensive diagnostic point-of-care tests (POCTs) are essential for controlling infectious diseases in resource-limited settings. In this study, we developed a new detection system based on nanoparticle-DNA aggregation (STat aggregation of tagged DNA, STAT-DNA) to yield a visual change that can be easily detected by the naked eye. This simplified optical detection system was applied to detect hepatitis C virus (HCV). Reverse transcription-polymerase chain reaction (RT-PCR) was performed using primers labeled with biotin and digoxigenin. Streptavidin-coated magnetic particles (1 μm) and anti-digoxigenin antibody-coated polystyrene particles (250-350 nm) were added to form aggregates. The limit of detection (LoD) and analytical specificity were analyzed. The STAT-DNA results were compared with those of the standard real-time PCR assay using serum samples from 54 patients with hepatitis C. We achieved visualization of amplified DNA with the naked eye by adding nanoparticles to the PCR mixture without employing centrifugal force, probe addition, incubation, or dilution. The LoD of STAT-DNA was at least 10
1 IU/mL. STAT-DNA did not show cross-reactivity with eight viral pathogens. The detection using STAT-DNA was consistent with that using standard real-time PCR.- Published
- 2022
- Full Text
- View/download PDF
46. Crystal Structure of the Recombination Mediator Protein RecO from Campylobacter jejuni and Its Interaction with DNA and a Zinc Ion.
- Author
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Lee SJ, Oh HB, and Yoon SI
- Subjects
- Bacterial Proteins metabolism, DNA chemistry, DNA, Single-Stranded, DNA-Binding Proteins metabolism, Zinc, Campylobacter jejuni genetics, Campylobacter jejuni metabolism
- Abstract
Homologous recombination is involved in repairing DNA damage, contributing to maintaining the integrity and stability of viral and cellular genomes. In bacteria, the recombination mediator proteins RecO and RecR are required to load the RecA recombinase on ssDNA for homologous recombination. To structurally and functionally characterize RecO, we determined the crystal structure of RecO from Campylobacter jejuni (cjRecO) at a 1.8 Å resolution and biochemically assessed its capacity to interact with DNA and a metal ion. cjRecO folds into a curved rod-like structure that consists of an N-terminal domain (NTD), C-terminal domain (CTD), and Zn
2+ -binding domain (ZnD). The ZnD at the end of the rod-like structure coordinates three cysteine residues and one histidine residue to accommodate a Zn2+ ion. Based on an extensive comparative analysis of RecO structures and sequences, we propose that the Zn2+ -binding consensus sequence of RecO is CxxC…C/HxxC/H/D. The interaction with Zn2+ is indispensable for the protein stability of cjRecO but does not seem to be required for the recombination mediator function. cjRecO also interacts with ssDNA as part of its biological function, potentially using the positively charged patch in the NTD and CTD. However, cjRecO displays a low ssDNA-binding affinity, suggesting that cjRecO requires RecR to efficiently recognize ssDNA for homologous recombination.- Published
- 2022
- Full Text
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47. Revealing Unknown Controlled Substances and New Psychoactive Substances Using High-Resolution LC-MS-MS Machine Learning Models and the Hybrid Similarity Search Algorithm.
- Author
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Lee SY, Lee ST, Suh S, Ko BJ, and Oh HB
- Subjects
- Algorithms, Artificial Intelligence, Chromatography, Liquid methods, Machine Learning, Psychotropic Drugs analysis, Reproducibility of Results, Controlled Substances, Tandem Mass Spectrometry methods
- Abstract
High-resolution liquid chromatography-tandem mass spectrometry (LC--MS-MS)-based machine learning models are constructed to address the analytical challenge of identifying unknown controlled substances and new psychoactive substances (NPSs). Using a training set composed of 770 LC-MS-MS barcode spectra (with binary entries 0 or 1) obtained generally by high-resolution mass spectrometers, three classification machine learning models were generated and evaluated. The three models are artificial neural network (ANN), support vector machine (SVM) and k-nearest neighbor (k-NN) models. In these models, controlled substances and NPSs were classified into 13 subgroups (benzylpiperazine, opiate, benzodiazepine, amphetamine, cocaine, methcathinone, classical cannabinoid, fentanyl, 2C series, indazole carbonyl compound, indole carbonyl compound, phencyclidine and others). Using 193 LC-MS-MS barcode spectra as an external test set, accuracy of the ANN, SVM and k-NN models were evaluated as 72.5%, 90.0% and 94.3%, respectively. Also, the hybrid similarity search (HSS) algorithm was evaluated to examine whether this algorithm can successfully identify unknown controlled substances and NPSs whose data are unavailable in the database. When only 24 representative LC-MS-MS spectra of controlled substances and NPSs were selectively included in the database, it was found that HSS can successfully identify compounds with high reliability. The machine learning models and HSS algorithms are incorporated into our home-coded artificial intelligence screener for narcotic drugs and psychotropic substances standalone software that is equipped with a graphic user interface. The use of this software allows unknown controlled substances and NPSs to be identified in a convenient manner., (© The Author(s) 2021. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2022
- Full Text
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48. The novel HLA-C*06:325 allele identified in a Korean individual awaiting kidney transplantation.
- Author
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Yang JJ, Kwon OJ, and Oh HB
- Subjects
- Alleles, Humans, Republic of Korea, Sequence Analysis, DNA, HLA-C Antigens genetics, Kidney Transplantation
- Abstract
HLA-C*06:325 differs from HLA-C*06:02:01:01 by a non-synonymous nucleotide substitution in codon 145, changing Arginine to Histidine., (© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2022
- Full Text
- View/download PDF
49. Structural and biochemical analyses of the flagellar expression regulator DegU from Listeria monocytogenes.
- Author
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Oh HB, Lee SJ, and Yoon SI
- Subjects
- Bacterial Proteins metabolism, DNA metabolism, Flagella physiology, Gene Expression Regulation, Bacterial, Promoter Regions, Genetic, Listeria monocytogenes metabolism
- Abstract
Listeria monocytogenes is a pathogenic bacterium that produces flagella, the locomotory organelles, in a temperature-dependent manner. At 37 °C inside humans, L. monocytogenes employs MogR to repress the expression of flagellar proteins, thereby preventing the production of flagella. However, in the low-temperature environment outside of the host, the antirepressor GmaR inactivates MogR, allowing flagellar formation. Additionally, DegU is necessary for flagellar expression at low temperatures. DegU transcriptionally activates the expression of GmaR and flagellar proteins by binding the operator DNA in the fliN-gmaR promoter as a response regulator of a two-component regulatory system. To determine the DegU-mediated regulation mechanism, we performed structural and biochemical analyses on the recognition of operator DNA by DegU. The DegU-DNA interaction is primarily mediated by a C-terminal DNA-binding domain (DBD) and can be fortified by an N-terminal receiver domain (RD). The DegU DBD adopts a tetrahelical helix-turn-helix structure and assembles into a dimer. The DegU DBD dimer recognizes the operator DNA using a positive patch. Unexpectedly, unlike typical response regulators, DegU interacts with operator DNA in both unphosphorylated and phosphorylated states with similar binding affinities. Therefore, we conclude that DegU is a noncanonical response regulator that is constitutively active irrespective of phosphorylation., (© 2022. The Author(s).)
- Published
- 2022
- Full Text
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50. Outcome of ABO-Incompatible Kidney Transplantation According to ABO Type of Transfused Plasma: Comparative Analysis Between "Universal" AB and Donor-Type Plasma.
- Author
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Kim HJ, Kim JS, Yang JJ, Chung Y, Kim H, Shin S, Kim YH, Hwang SH, Oh HB, Han DJ, Kwon H, and Ko DH
- Subjects
- ABO Blood-Group System, Blood Group Incompatibility, Graft Rejection, Graft Survival, Humans, Living Donors, Retrospective Studies, Kidney Transplantation
- Abstract
Objective: We compared the clinical outcomes of recipients of ABO-incompatible (ABOi) kidney transplantation (KT) according to the blood group of the plasma transfused., Materials and Methods: We retrospectively analyzed the data of 60 recipients of ABOi-KT with blood type O and A or B donors. Demographic and clinical characteristics were compared between 2 groups of recipients: 1 group received AB plasma regardless of the donor's blood type (n = 30), and the other group received donor-type plasma (n = 30)., Results: There were no significant differences between the groups in terms of demographic characteristics. Transfusion of donor-type plasma was noninferior to transfusion of type AB plasma in terms of both rejection-free survival and rejection rate (P = .455, P = .335)., Conclusion: There was no significant prognostic difference between the 2 groups. In terms of blood supply and inventory management, we suggest that the blood group of the plasma should match the donor's type., (© The Author(s) 2022. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2022
- Full Text
- View/download PDF
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