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1. Bioprocessing considerations for generation of iPSCs intended for clinical application: perspectives from the ISCT Emerging Regenerative Medicine Technology working group

Author

Song, Hannah W., Solomon, Jennifer N., Masri, Fernanda, Ph.D., Mack, Amanda, Durand, Nisha, Cameau, Emmanuelle, Dianat, Noushin, Hunter, Arwen, Oh, Steve, Schoen, Brianna, Marsh, Matthew, Bravery, Christopher, Sumen, Cenk, Clarke, Dominic, Bharti, Kapil, Allickson, Julie G., and Lakshmipathy, Uma

Published
2024
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7. Strategic Considerations For Korean Investors - Leveraging The Special Tax Provision For Foreign Pass-Through Entities

Author

Oh, Steve H.

Subjects
Foreign tax credit -- Laws, regulations and rules, Corporate income taxes -- Laws, regulations and rules, Government regulation, Business, international
Abstract

It has been a year since the introduction of the special tax provision that enables taxpayers to treat foreign pass-through entities, which may not be considered as such in Korea, [...]

Published
2024

9. The Diversity, Structure, and Function of Heritable Adaptive Immunity Sequences in the Aedes aegypti Genome

Author

Whitfield, Zachary J, Dolan, Patrick T, Kunitomi, Mark, Tassetto, Michel, Seetin, Matthew G, Oh, Steve, Heiner, Cheryl, Paxinos, Ellen, and Andino, Raul

Subjects
Genetics, Emerging Infectious Diseases, Vector-Borne Diseases, Vaccine Related, Biodefense, Infectious Diseases, Prevention, Biotechnology, Underpinning research, 1.1 Normal biological development and functioning, Infection, Good Health and Well Being, Adaptive Immunity, Aedes, Animals, DNA Transposable Elements, Genome, Mosquito Vectors, RNA Interference, RNA, Small Interfering, Aedes aegypti, EVE, endogenous viral element, piRNA clusters, retrotransposon, small RNAs, transposable element, transposon, Biological Sciences, Medical and Health Sciences, Psychology and Cognitive Sciences, Developmental Biology
Abstract

The Aedes aegypti mosquito transmits arboviruses, including dengue, chikungunya, and Zika virus. Understanding the mechanisms underlying mosquito immunity could provide new tools to control arbovirus spread. Insects exploit two different RNAi pathways to combat viral and transposon infection: short interfering RNAs (siRNAs) and PIWI-interacting RNAs (piRNAs) [1, 2]. Endogenous viral elements (EVEs) are sequences from non-retroviral viruses that are inserted into the mosquito genome and can act as templates for the production of piRNAs [3, 4]. EVEs therefore represent a record of past infections and a reservoir of potential immune memory [5]. The large-scale organization of EVEs has been difficult to resolve with short-read sequencing because they tend to integrate into repetitive regions of the genome. To define the diversity, organization, and function of EVEs, we took advantage of the contiguity associated with long-read sequencing to generate a high-quality assembly of the Ae. aegypti-derived Aag2 cell line genome, an important and widely used model system. We show EVEs are acquired through recombination with specific classes of long terminal repeat (LTR) retrotransposons and organize into large loci (>50 kbp) characterized by high LTR density. These EVE-containing loci have increased density of piRNAs compared to similar regions without EVEs. Furthermore, we detected EVE-derived piRNAs consistent with a targeted processing of persistently infecting virus genomes. We propose that comparisons of EVEs across mosquito populations may explain differences in vector competence, and further study of the structure and function of these elements in the genome of mosquitoes may lead to epidemiological interventions.

Published
2017

10. Scalable mesenchymal stem cell enrichment from bone marrow aspirate using deterministic lateral displacement (DLD) microfluidic sorting

Author

Tan Kwan Zen, Nicholas, Zeming, Kerwin Kwek, Teo, Kim Leng, Loberas, Mavis, Lee, Jialing, Goh, Chin Ren, Yang, Da Hou, Oh, Steve, Hui Hoi Po, James, Cool, Simon M., Hou, Han Wei, Han, Jongyoon, Tan Kwan Zen, Nicholas, Zeming, Kerwin Kwek, Teo, Kim Leng, Loberas, Mavis, Lee, Jialing, Goh, Chin Ren, Yang, Da Hou, Oh, Steve, Hui Hoi Po, James, Cool, Simon M., Hou, Han Wei, and Han, Jongyoon

Abstract

The growing interest in regenerative medicine has opened new avenues for novel cell therapies using stem cells. Bone marrow aspirate (BMA) is an important source of stromal mesenchymal stem cells (MSCs). Conventional MSC harvesting from BMA relies on archaic centrifugation methods, often leading to poor yield due to osmotic stress, high centrifugation force, convoluted workflow, and long experimental time (∼2–3 hours). To address these issues, we have developed a scalable microfluidic technology based on deterministic lateral displacement (DLD) for MSC isolation. This passive, label-free cell sorting method capitalizes on the morphological differences between MSCs and blood cells (platelets and RBCs) for effective separation using an inverted L-shaped pillar array. To improve throughput, we developed a novel multi-chip DLD system that can process 2.5 mL of raw BMA in 20 ± 5 minutes, achieving a 2-fold increase in MSC recovery compared to centrifugation methods. Taken together, we envision that the developed DLD platform will enable fast and efficient isolation of MSCs from BMA for effective downstream cell therapy in clinical settings., National Research Foundation Singapore

Published
2024

21. A physical sciences network characterization of non-tumorigenic and metastatic cells

Author

Agus, David B, Alexander, Jenolyn F, Arap, Wadih, Ashili, Shashanka, Aslan, Joseph E, Austin, Robert H, Backman, Vadim, Bethel, Kelly J, Bonneau, Richard, Chen, Wei-Chiang, Chen-Tanyolac, Chira, Choi, Nathan C, Curley, Steven A, Dallas, Matthew, Damania, Dhwanil, Davies, Paul CW, Decuzzi, Paolo, Dickinson, Laura, Estevez-Salmeron, Luis, Estrella, Veronica, Ferrari, Mauro, Fischbach, Claudia, Foo, Jasmine, Fraley, Stephanie I, Frantz, Christian, Fuhrmann, Alexander, Gascard, Philippe, Gatenby, Robert A, Geng, Yue, Gerecht, Sharon, Gillies, Robert J, Godin, Biana, Grady, William M, Greenfield, Alex, Hemphill, Courtney, Hempstead, Barbara L, Hielscher, Abigail, Hillis, W Daniel, Holland, Eric C, Ibrahim-Hashim, Arig, Jacks, Tyler, Johnson, Roger H, Joo, Ahyoung, Katz, Jonathan E, Kelbauskas, Laimonas, Kesselman, Carl, King, Michael R, Konstantopoulos, Konstantinos, Kraning-Rush, Casey M, Kuhn, Peter, Kung, Kevin, Kwee, Brian, Lakins, Johnathon N, Lambert, Guillaume, Liao, David, Licht, Jonathan D, Liphardt, Jan T, Liu, Liyu, Lloyd, Mark C, Lyubimova, Anna, Mallick, Parag, Marko, John, McCarty, Owen JT, Meldrum, Deirdre R, Michor, Franziska, Mumenthaler, Shannon M, Nandakumar, Vivek, O'Halloran, Thomas V, Oh, Steve, Pasqualini, Renata, Paszek, Matthew J, Philips, Kevin G, Poultney, Christopher S, Rana, Kuldeepsinh, Reinhart-King, Cynthia A, Ros, Robert, Semenza, Gregg L, Senechal, Patti, Shuler, Michael L, Srinivasan, Srimeenakshi, Staunton, Jack R, Stypula, Yolanda, Subramanian, Hariharan, Tlsty, Thea D, Tormoen, Garth W, Tseng, Yiider, van Oudenaarden, Alexander, Verbridge, Scott S, Wan, Jenny C, Weaver, Valerie M, Widom, Jonathan, Will, Christine, Wirtz, Denis, Wojtkowiak, Jonathan, and Wu, Pei-Hsun

Subjects
Biochemistry and Cell Biology, Biological Sciences, Cancer, Breast Cancer, Biotechnology, Aetiology, 2.1 Biological and endogenous factors, Biomarkers, Tumor, Cell Line, Tumor, Cell Movement, Cell Size, Cell Survival, Computer Simulation, Gene Expression Regulation, Neoplastic, Humans, Models, Biological, Neoplasm Metastasis, Neoplasm Proteins, Physical Sciences - Oncology Centers Network
Abstract

To investigate the transition from non-cancerous to metastatic from a physical sciences perspective, the Physical Sciences-Oncology Centers (PS-OC) Network performed molecular and biophysical comparative studies of the non-tumorigenic MCF-10A and metastatic MDA-MB-231 breast epithelial cell lines, commonly used as models of cancer metastasis. Experiments were performed in 20 laboratories from 12 PS-OCs. Each laboratory was supplied with identical aliquots and common reagents and culture protocols. Analyses of these measurements revealed dramatic differences in their mechanics, migration, adhesion, oxygen response, and proteomic profiles. Model-based multi-omics approaches identified key differences between these cells' regulatory networks involved in morphology and survival. These results provide a multifaceted description of cellular parameters of two widely used cell lines and demonstrate the value of the PS-OC Network approach for integration of diverse experimental observations to elucidate the phenotypes associated with cancer metastasis.

Published
2013

22. A physical sciences network characterization of non-tumorigenic and metastatic cells.

Author

Physical Sciences - Oncology Centers Network, Agus, David B, Alexander, Jenolyn F, Arap, Wadih, Ashili, Shashanka, Aslan, Joseph E, Austin, Robert H, Backman, Vadim, Bethel, Kelly J, Bonneau, Richard, Chen, Wei-Chiang, Chen-Tanyolac, Chira, Choi, Nathan C, Curley, Steven A, Dallas, Matthew, Damania, Dhwanil, Davies, Paul CW, Decuzzi, Paolo, Dickinson, Laura, Estevez-Salmeron, Luis, Estrella, Veronica, Ferrari, Mauro, Fischbach, Claudia, Foo, Jasmine, Fraley, Stephanie I, Frantz, Christian, Fuhrmann, Alexander, Gascard, Philippe, Gatenby, Robert A, Geng, Yue, Gerecht, Sharon, Gillies, Robert J, Godin, Biana, Grady, William M, Greenfield, Alex, Hemphill, Courtney, Hempstead, Barbara L, Hielscher, Abigail, Hillis, W Daniel, Holland, Eric C, Ibrahim-Hashim, Arig, Jacks, Tyler, Johnson, Roger H, Joo, Ahyoung, Katz, Jonathan E, Kelbauskas, Laimonas, Kesselman, Carl, King, Michael R, Konstantopoulos, Konstantinos, Kraning-Rush, Casey M, Kuhn, Peter, Kung, Kevin, Kwee, Brian, Lakins, Johnathon N, Lambert, Guillaume, Liao, David, Licht, Jonathan D, Liphardt, Jan T, Liu, Liyu, Lloyd, Mark C, Lyubimova, Anna, Mallick, Parag, Marko, John, McCarty, Owen JT, Meldrum, Deirdre R, Michor, Franziska, Mumenthaler, Shannon M, Nandakumar, Vivek, O'Halloran, Thomas V, Oh, Steve, Pasqualini, Renata, Paszek, Matthew J, Philips, Kevin G, Poultney, Christopher S, Rana, Kuldeepsinh, Reinhart-King, Cynthia A, Ros, Robert, Semenza, Gregg L, Senechal, Patti, Shuler, Michael L, Srinivasan, Srimeenakshi, Staunton, Jack R, Stypula, Yolanda, Subramanian, Hariharan, Tlsty, Thea D, Tormoen, Garth W, Tseng, Yiider, van Oudenaarden, Alexander, Verbridge, Scott S, Wan, Jenny C, Weaver, Valerie M, Widom, Jonathan, Will, Christine, Wirtz, Denis, Wojtkowiak, Jonathan, and Wu, Pei-Hsun

Subjects
Physical Sciences - Oncology Centers Network, Cell Line, Tumor, Humans, Neoplasm Metastasis, Neoplasm Proteins, Cell Movement, Cell Size, Cell Survival, Gene Expression Regulation, Neoplastic, Models, Biological, Computer Simulation, Biomarkers, Tumor, Cancer, Breast Cancer, Biotechnology, 2.1 Biological and endogenous factors
Abstract

To investigate the transition from non-cancerous to metastatic from a physical sciences perspective, the Physical Sciences-Oncology Centers (PS-OC) Network performed molecular and biophysical comparative studies of the non-tumorigenic MCF-10A and metastatic MDA-MB-231 breast epithelial cell lines, commonly used as models of cancer metastasis. Experiments were performed in 20 laboratories from 12 PS-OCs. Each laboratory was supplied with identical aliquots and common reagents and culture protocols. Analyses of these measurements revealed dramatic differences in their mechanics, migration, adhesion, oxygen response, and proteomic profiles. Model-based multi-omics approaches identified key differences between these cells' regulatory networks involved in morphology and survival. These results provide a multifaceted description of cellular parameters of two widely used cell lines and demonstrate the value of the PS-OC Network approach for integration of diverse experimental observations to elucidate the phenotypes associated with cancer metastasis.

Published
2013

23. An analogy between the evolution of drug resistance in bacterial communities and malignant tissues

Author

Lambert, Guillaume, Estévez-Salmeron, Luis, Oh, Steve, Liao, David, Emerson, Beverly M, Tlsty, Thea D, and Austin, Robert H

Subjects
Biomedical and Clinical Sciences, Oncology and Carcinogenesis, Antimicrobial Resistance, Infectious Diseases, Evaluation of treatments and therapeutic interventions, Development of treatments and therapeutic interventions, 6.1 Pharmaceuticals, 5.1 Pharmaceuticals, Cancer, Infection, Antineoplastic Agents, Drug Resistance, Bacterial, Humans, Models, Biological, Neoplasms, Medical and Health Sciences, Oncology & Carcinogenesis, Biomedical and clinical sciences, Health sciences
Abstract

Cancer cells rapidly evolve drug resistance through somatic evolution and, in order to continue growth in the metastatic phase, violate the organism-wide consensus of regulated growth and beneficial communal interactions. We suggest that there is a fundamental mechanistic connection between the rapid evolution of resistance to chemotherapy in cellular communities within malignant tissues and the rapid evolution of antibiotic resistance in bacterial communities. We propose that this evolution is the result of a programmed and collective stress response performed by interacting cells, and that, given this fundamental connection, studying bacterial communities can provide deeper insights into the dynamics of adaptation and the evolution of cells within tumours.

Published
2011

26. Dopamine transporter neuroimaging accurately assesses the maturation of dopamine neurons in a preclinical model of Parkinson’s disease

Author

Goggi, Julian L., Qiu, Lifeng, Liao, Mei Chih, Khanapur, Shivashankar, Jiang, Lingfan, Boominathan, Ramasamy, Hartimath, Siddesh V., Cheng, Peter, Yong, Fui Fong, Soh, Vanessa, Deng, Xiaozhou, Lin, Youshan Melissa, Haslop, Anna, Tan, Peng Wen, Zeng, Xiaoxia, Lee, Jolene W. L., Zhang, Zhiwei, Sadasivam, Pragalath, Tan, Eng King, Luthra, Sajinder K., Shingleton, William D., Oh, Steve K. W., Zeng, Li, and Robins, Edward G.

Published
2020
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28. Scalable Mesenchymal Stem Cells Enrichment from Bone Marrow Aspirate using Deterministic Lateral Displacement (DLD) Microfluidics Sorting

Author

Tan, Nicholas, primary, Kwek, Kerwin Zeming, additional, Teo, Kim Leng, additional, Loberas, Mavis, additional, Lee, Jia Ling, additional, Goh, Chin Ren, additional, Yang, Dahou, additional, Oh, Steve, additional, Hui, James, additional, Cool, Simon, additional, Wei Hou, Han, additional, and Han, Jongyoon, additional

Published
2023
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33. International Society for Cellular Therapy perspective on immune functional assays for mesenchymal stromal cells as potency release criterion for advanced phase clinical trials

Author

Galipeau, Jacques, Krampera, Mauro, Barrett, John, Dazzi, Francesco, Deans, Robert J., DeBruijn, Joost, Dominici, Massimo, Fibbe, Willem E., Gee, Adrian P., Gimble, Jeffery M., Hematti, Peiman, Koh, Mickey B.C., LeBlanc, Katarina, Martin, Ivan, McNiece, Ian K., Mendicino, Michael, Oh, Steve, Ortiz, Luis, Phinney, Donald G., Planat, Valerie, Shi, Yufang, Stroncek, David F., Viswanathan, Sowmya, Weiss, Daniel J., and Sensebe, Luc

Published
2016
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34. Scalable mesenchymal stem cell enrichment from bone marrow aspirate using deterministic lateral displacement (DLD) microfluidic sorting.

Author

Tan Kwan Zen, Nicholas, Zeming, Kerwin Kwek, Teo, Kim Leng, Loberas, Mavis, Lee, Jialing, Goh, Chin Ren, Yang, Da Hou, Oh, Steve, Hui Hoi Po, James, Cool, Simon M., Hou, Han Wei, and Han, Jongyoon

Subjects
BONE marrow, BLOOD cells, MESENCHYMAL stem cells, STEM cell treatment, REGENERATIVE medicine, HARVESTING, CENTRIFUGATION
Abstract

The growing interest in regenerative medicine has opened new avenues for novel cell therapies using stem cells. Bone marrow aspirate (BMA) is an important source of stromal mesenchymal stem cells (MSCs). Conventional MSC harvesting from BMA relies on archaic centrifugation methods, often leading to poor yield due to osmotic stress, high centrifugation force, convoluted workflow, and long experimental time (∼2–3 hours). To address these issues, we have developed a scalable microfluidic technology based on deterministic lateral displacement (DLD) for MSC isolation. This passive, label-free cell sorting method capitalizes on the morphological differences between MSCs and blood cells (platelets and RBCs) for effective separation using an inverted L-shaped pillar array. To improve throughput, we developed a novel multi-chip DLD system that can process 2.5 mL of raw BMA in 20 ± 5 minutes, achieving a 2-fold increase in MSC recovery compared to centrifugation methods. Taken together, we envision that the developed DLD platform will enable fast and efficient isolation of MSCs from BMA for effective downstream cell therapy in clinical settings. [ABSTRACT FROM AUTHOR]

Published
2023
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49. Report of the International Stem Cell Banking Initiative Workshop Activity: Current Hurdles and Progress in Seed‐Stock Banking of Human Pluripotent Stem Cells

Author

Kim, Jung‐Hyun, Kurtz, Andreas, Yuan, Bao‐Zhu, Zeng, Fanyi, Lomax, Geoff, Loring, Jeanne F., Crook, Jeremy, Ju, Ji Hyeon, Clarke, Laura, Inamdar, Maneesha S., Pera, Martin, Firpo, Meri T., Sheldon, Michael, Rahman, Nafees, OʼShea, Orla, Pranke, Patricia, Zhou, Qi, Isasi, Rosario, Rungsiwiwut, Ruttachuk, Kawamata, Shin, Oh, Steve, Ludwig, Tenneille, Masui, Tohru, Novak, Thomas J., Takahashi, Tsuneo, Fujibuchi, Wataru, Koo, Soo Kyung, and Stacey, Glyn N.

Published
2017
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