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One of the omega-3 essential fatty acids, docosahexaenoic acid (DHA), is a significant constituent of the cell membrane and the precursor of several potent lipid mediators. These mediators are considered to be important in preventing or treating several diseases. Resolvin D5, an oxidized lipid mediator derived from DHA, has been known to exert anti-inflammatory effects. However, the detailed mechanism underlying these effects has not yet been elucidated in human monocytic THP-1 cells. In the present study, we investigated the effects of resolvin D5 on inflammation-related signaling pathways, including the extracellular signal-regulated kinase (ERK)-nuclear factor (NF)-κB signaling pathway. Resolvin D5 downregulated the production of interleukin (IL)-6 and chemokine (C-C motif) ligand 5 (CCL5). Additionally, these inhibitory effects were found to be modulated by mitogen-activated protein kinase (MAPK) and NF-κB in lipopolysaccharide (LPS)-treated THP-1 cells. Resolvin D5 inhibited the LPS-stimulated phosphorylation of ERK and translocation of p65 and p50 into the nucleus, resulting in the inhibition of IL-6 and CCL5 production. These results revealed that resolvin D5 exerts anti-inflammatory effects in LPS-treated THP-1 cells by regulating the phosphorylation of ERK and nuclear translocation of NF-kappaB.

Background: Although a laparoscopic cholecystectomy (LC) is the gold standard treatment for symptomatic cholelithiasis, its safety and efficacy in the morbidly/super obese patients is unknown. The aim of this study was to investigate the safety and efficacy of an elective LC in the morbid/super obese patients., Methods: A retrospective review of the hospital electronic database and medical records was conducted searching for all elective LC from 2010 to 2013. The data collected included patient demographics and body mass index (BMI), length of hospital stay (LOS), duration of surgery (DOS), intra- and post-operative complications, bile duct injuries, performance of an intra-operative cholangiogram, the incidence of open conversion and the seniority of the operator., Results: A total of 799 patients (76% female) with a mean age of 46 years and BMI of 31 were included in this study. There were significant differences in the median DOS between the three BMI groups; BMI < 26 [64 min; interquartile range (IQR) 54-83]; BMI 26-40 (72 min, IQR 58-91) and BMI > 40 (82 min, IQR 63-104), P < 0.001. There were no statistically significant differences in the LOS, peri-operative complication rates, open conversions or bile duct injuries among the BMI groups., Conclusions: This study showed that LC can be performed safely in the morbid/super obese patients., (© 2015 International Hepato-Pancreato-Biliary Association.)

Herpes simplex virus (HSV) has been studied in well-established mouse models to generate latently infected animals for investigations into viral pathogenesis, latency mechanisms, and reactivation. Mice exhibit clinical signs of debilitating infection, during which time they may become severely ill before recovery or die spontaneously. Because the cohort of mice that does survive provides valuable data on latency, there is keen interest in developing methodologies for earlier detection and treatment of severe disease to ultimately increase survival rates. Here, BALB/c mice were inoculated ocularly with either a wildtype (LAT(+)) or mutant (LAT(-)) strain of HSV1. Mice were monitored daily through day 30 after infection; trigeminal ganglia were harvested at day 60 to assess viral DNA load. Cages were provided with nesting material, and fluid supplementation was administered to mice with body temperatures of 35 °C or lower, as measured by subcutaneous microchip thermometry. The results showed that infected mice with temperatures less than 34.5 °C did not recover to normothermia and were euthanized or spontaneously died, regardless of infective viral strain. By using a combination of criteria including body temperature (less than 34.5 °C) and weight loss (more than 0.05 g daily) for removal of animals from the study, approximately 98% of mice that died spontaneously could have been euthanized prior to death, without concern of potential recovery to the experimental endpoint (100% specificity). Frequent monitoring of alterations to general wellbeing, body temperature, and weight was crucial for establishing humane endpoints in this ocular HSV model.