21 results on '"Ogechukwu Eze"'
Search Results
2. Hepatic Resection and Transplant in Glycogen Storage Diseases
- Author
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Kenneth Washburn, Ogechukwu Eze, Elmahdi A. Elkhammas, Anthony Michaels, Musab Alebrahim, Sylvester M. Black, Austin Schenk, Ashraf El-Hinnawi, Navdeep Singh, and Bryan Hill
- Subjects
Transplantation ,medicine.medical_specialty ,Glycogen ,business.industry ,Hepatic resection ,medicine.medical_treatment ,Blood sugar ,medicine.disease ,Surgery ,chemistry.chemical_compound ,chemistry ,Lactic acidosis ,Medicine ,Glycogen storage disease ,Hepatectomy ,medicine.symptom ,business ,Severe lactic acidosis ,Acidosis - Abstract
Patients with glycogen storage diseases pose unique management challenges to clinicians.These challenges are exacerbated wheneverthey undergo surgery as the basic anomaly in their glycogen storage pathways make them susceptible to organic acidosis, which may in turn complicate their preoperative, intraoperative, and postoperative course. Because of the rarity of these diseases, clinicians may not be aware of the specific management concerns. In the case reported here, a 37-year-old patient with glycogen storage disease type 1 underwentleft hepatectomy for hepatic adenomatosis, which was complicated by intraoperative severe lactic acidosis that was successfully treated. After successful hepatectomy, the patient underwent liver transplant without major lactic acidosis or hemodynamic instability. Early recognition and aggressive management of blood sugar and lactic acidosis in patients with glycogen storage diseases can allow for successful outcomes even when complex surgical procedures are required.
- Published
- 2022
3. Definition of Barrett Esophagus in the United States
- Author
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Yaman Tarabishy, Elizabeth A. Montgomery, Kevin M. Waters, Maryam Kherad Pezhouh, Marcia I. Canto, Kevan J. Salimian, Lysandra Voltaggio, Eun Ji Shin, and Ogechukwu Eze
- Subjects
Adult ,Male ,medicine.medical_specialty ,Biopsy ,medicine.medical_treatment ,Gastroenterology ,Pathology and Forensic Medicine ,Barrett Esophagus ,Young Adult ,03 medical and health sciences ,Esophagus ,0302 clinical medicine ,Predictive Value of Tests ,Terminology as Topic ,Internal medicine ,Metaplasia ,medicine ,Humans ,Young adult ,Aged ,Retrospective Studies ,Aged, 80 and over ,Chemotherapy ,medicine.diagnostic_test ,business.industry ,Intestinal metaplasia ,Retrospective cohort study ,Middle Aged ,medicine.disease ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Predictive value of tests ,Baltimore ,Female ,030211 gastroenterology & hepatology ,Surgery ,Goblet Cells ,Anatomy ,medicine.symptom ,business - Abstract
Barrett esophagus (BE) predisposes patients to the development of esophageal adenocarcinoma (EAC). However, the global definition of BE is controversial. Pathologists in Europe and the United States require intestinal metaplasia (IM) within columnar-lined mucosa (CLM) in the tubular esophagus to diagnose BE, whereas in the UK and Japan only the presence of CLM is required. To aid in establishing an appropriate definition for BE, we evaluated whether IM accompanies EAC in a US patient cohort. We examined a series of 139 consecutive patients who underwent endoscopic mucosal resections or esophagectomies for EAC performed at a US tertiary care center. The resection specimens were evaluated for the presence (IM+) or absence (IM-) of IM within CLM. Ninety-seven (70%) patients were IM+. Tumors found in IM- patients tended to be advanced at the time of resection (57% pT3 or greater, IM-; 31% pT3 or greater, IM+; P=0.02) such that the tumor may have "overgrown" zones of IM. We hypothesized that changes as a result of neoadjuvant chemotherapy or radiation might mask preexisting IM. When evaluating this hypothesis, we found that 34 of 39 of treatment-naive patients were IM+. Two of the 5 IM- patients had prior IM+ biopsies resulting in 92% of treatment-naive patients who were IM+. In our US hospital population, CLM with IM in the tubular esophagus is found in association with EAC in 70% to 92% of patients. We believe that based on these data the United States definition of BE should continue to require the presence of IM.
- Published
- 2018
4. A practical approach for diagnosis of appendiceal mucinous neoplasms
- Author
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Ogechukwu Eze, Elizabeth A. Montgomery, and Robert Jones
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Histology ,Low Grade Appendiceal Mucinous Neoplasm ,Peritoneal surface ,business.industry ,medicine.disease ,Pathology and Forensic Medicine ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,030220 oncology & carcinogenesis ,medicine ,Pseudomyxoma peritonei ,Radiology ,Multiple classification ,business - Abstract
Classification of appendiceal mucinous neoplasms remains controversial, and while multiple classification systems have been proposed, no single system is universally utilized. A recent consensus by the Peritoneal Surface Oncology Group International (PSOGI) provides clear diagnostic criteria for appendiceal mucinous neoplasms and their corresponding risk for pseudomyxoma peritonei. Here, we briefly review appendiceal mucinous neoplasms and associated risk for pseudomyxoma peritonei, and discuss the diagnostic difficulties, our approach, and the reporting guidelines proposed.
- Published
- 2017
5. Refractory inflammatory bowel disease: is there a role for Epstein-Barr virus? A case-controlled study using highly sensitive Epstein-Barr virus-encoded small RNA1 in situ hybridization
- Author
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Maryam Kherad Pezhouh, James A. Miller, Ogechukwu Eze, Mark Lazarev, Maria Westerhoff, Kevin M. Waters, Lysandra Voltaggio, Rajni Sharma, Alyssa Parian, and David Borzik
- Subjects
Adult ,Male ,medicine.medical_specialty ,Epstein-Barr Virus Infections ,Herpesvirus 4, Human ,Adolescent ,Colon ,medicine.medical_treatment ,Biopsy ,Endometriosis ,Drug Resistance ,medicine.disease_cause ,Inflammatory bowel disease ,Gastroenterology ,Severity of Illness Index ,Pathology and Forensic Medicine ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Refractory ,Crohn Disease ,Gastrointestinal Agents ,Risk Factors ,hemic and lymphatic diseases ,Internal medicine ,medicine ,Humans ,Child ,Colectomy ,In Situ Hybridization ,Aged ,business.industry ,Case-control study ,Middle Aged ,medicine.disease ,Epstein–Barr virus ,Ulcerative colitis ,digestive system diseases ,United States ,Dysplasia ,030220 oncology & carcinogenesis ,Case-Control Studies ,RNA, Viral ,030211 gastroenterology & hepatology ,Colitis, Ulcerative ,Female ,business - Abstract
A potential role for viral infections has been implicated in inflammatory bowel disease (IBD) unresponsive to medical treatment. It is well known that Epstein-Barr virus (EBV) infection can elicit a brisk mononuclear response in the gastrointestinal tract. The aim of this study was to further evaluate the role of EBV in patients with refractory IBD and compare them with nonrefractory IBD cases. Surgically resected colonic specimens from 67 patients with refractory IBD (62 with ulcerative colitis, 3 patients with Crohn disease, and 2 patients with indeterminate colitis) were retrieved. Twelve colectomy specimens from patients with ulcerative colitis who had undergone resections for dysplasia or endometriosis were included as controls. Highly sensitive EBV-encoded small RNA1 (EBER-1) in situ hybridization was performed on a representative block from each specimen. EBER-1 reactivity was graded as absent, focal, or diffuse. EBV was detected in 60% (40/67) of patients with refractory IBD compared with 25% (3/12) of the control group (P < .05). Focal EBER-1 positivity was present in 45% of cases of refractory IBD compared with 25% of controls. Diffuse EBER-1 reactivity was seen in 15% of cases of refractory IBD (10/67); none of the samples from the control group contained diffuse EBER-1 positivity. There was a positive correlation between EBER positivity and depth of inflammation and mucosal ulceration in patients with refractory IBD. Our findings suggest a potential role for EBV infection in patients with refractory IBD.
- Published
- 2018
6. Frequency and pathological characteristics of drug-induced liver injury in a tertiary medical center
- Author
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Ogechukwu Eze, James S. Park, Shweta Gera, Mark Ettel, Gabriel Acosta Gonzalez, Ruliang Xu, and Samuel H. Sigal
- Subjects
Adult ,Male ,medicine.medical_specialty ,Biopsy ,Antineoplastic Agents ,Gastroenterology ,Pathology and Forensic Medicine ,Tertiary Care Centers ,03 medical and health sciences ,0302 clinical medicine ,Cholestasis ,Anti-Infective Agents ,Predictive Value of Tests ,Risk Factors ,Internal medicine ,medicine ,Electronic Health Records ,Humans ,Aged ,Hypolipidemic Agents ,Liver injury ,Hepatitis ,medicine.diagnostic_test ,business.industry ,Hepatobiliary disease ,Middle Aged ,medicine.disease ,Liver ,030220 oncology & carcinogenesis ,Liver biopsy ,Chemical and Drug Induced Liver Injury, Chronic ,Dietary Supplements ,030211 gastroenterology & hepatology ,Female ,New York City ,Plant Preparations ,Chemical and Drug Induced Liver Injury ,Liver function tests ,business ,Medication list ,Immunosuppressive Agents - Abstract
Drug-induced liver injury (DILI) accounts for approximately 10% of acute hepatitis cases. DILI can arise as idiosyncratic or intrinsic injury from hundreds of drugs, herbals, and nutritional supplements and is essential to recognize as one of the differential diagnoses of hepatitis in a liver biopsy. The purpose of this study is to investigate the frequency and pathological characteristics of DILI related to the variety of hepatotoxic agents. We searched our pathology database for all patients with hepatitis diagnosed on liver biopsy from January 2012 to May 2016, and selected patients with a diagnosis of DILI. Electronic medical records were reviewed for patient medication list, history of herbal medicine or supplement use, and pre-biopsy liver function test (LFT) results. Clinical and pathologic correlation was used to determine the causative or related agents for DILI. We then assessed histopathologic features of liver injury and categorized biopsy findings as primarily bile duct injury, lobular/portal hepatitis, or mixed changes. Six hundred four total liver biopsies for hepatitis or liver injury were identified, of which 70 cases (11.6%) carried the diagnosis of DILI confirmed by clinical correlation. The most common etiologies associated with DILI were supplements and herbal products (31.4%), antimicrobials (14.3%), chemotherapeutics (11.4%), antilipidemics (7.1%) and immunomodulatory agents (7.1%). LFT results positively correlated with histological findings. Nutritional/herbal supplements have emerged as one of the major hepatotoxicity agents. DILI can manifest as predominantly hepatitis, bile duct injury or combination. Histological pattern recognition in the liver biopsy may help identify specific hepatotoxic agents causing DILI.
- Published
- 2017
7. A rare case of Crohn’s ileitis in a patient with constitutional mismatch repair deficiency
- Author
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Ogechukwu Eze, Pavlos Kaimakliotis, Francis Giardiello, and Brindusa Truta
- Subjects
Crohn’s disease ,vedolizumab ,medicine.medical_specialty ,Colorectal cancer ,medicine.medical_treatment ,Case Report ,Malignancy ,familial cancer ,Gastroenterology ,Vedolizumab ,03 medical and health sciences ,Ileostomy ,0302 clinical medicine ,Internal medicine ,constitutional mismatch repair deficiency ,medicine ,Ileitis ,Crohn's disease ,business.industry ,medicine.disease ,Lynch syndrome ,030220 oncology & carcinogenesis ,030211 gastroenterology & hepatology ,business ,medicine.drug ,Rare disease - Abstract
Constitutional mismatch repair deficiency (CMMRD), a variant of Lynch syndrome, is a rare disease characterized by café-au-lait spots, oligopolyposis, glioblastoma and lymphoma. A 24-year-old male, under surveillance for CMMRD, developed Crohn’s ileitis after total colectomy with end ileostomy for colorectal cancer and failed to respond to oral corticosteroids. The patient underwent induction and maintenance of remission with vedolizumab infusions. We report the first patient with CMMRD developing Crohn’s disease. The choice of immunosuppressive therapy in these patients is challenging and needs to be made according to their risk for malignancy.
- Published
- 2017
8. Clinical and biological significance of precursor lesions of intrahepatic cholangiocarcinoma
- Author
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Ogechukwu Eze, Ruliang Xu, and Mark Ettel
- Subjects
Intraepithelial neoplasia ,medicine.medical_specialty ,Pathology ,Hepatology ,business.industry ,Bile duct ,Bile duct hamartoma ,Liver fluke ,medicine.disease ,Gastroenterology ,Pathogenesis ,medicine.anatomical_structure ,Editorial ,Internal medicine ,medicine ,Biliary Intraepithelial Neoplasia ,business ,Bile Duct Adenoma ,Intrahepatic Cholangiocarcinoma - Abstract
Cholangiocarcinoma (CC) is primarily a malignant tumor of older adults most prevalent in Southeast Asia, where liver fluke infestation is high. However the etiology in western countries is unknown. Although the incidence of extrahepatic cholangiocarcinoma has remained constant, incidence of intrahepatic CC (ICC) which differs in morphology, pathogenesis, risk factors, treatment and prognosis is increasing. While this increase is associated with hepatitis C virus infection, chronic nonalcoholic liver disease, obesity, and smoking, the pathogenesis of ICC and molecular alterations underlying the carcinogenesis are not completely elucidated. Benign biliary lesions such as biliary intraepithelial neoplasia, intraductal papillary neoplasm of the bile duct, von Meyenburg complex or bile duct hamartoma, and bile duct adenoma have been associated with ICC. For each of these entities, evidence suggests or supports a role as premalignant lesions. This article summarized the important biological significance of the precursor lesions of ICC and the molecular mechanisms that may be involved in intrahepatic cholangiocarcinogenesis.
- Published
- 2015
9. Colonic polyp presenting as a tubulovillous adenoma and harbinger of high-grade neuroendocrine carcinoma: a unique presentation
- Author
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Ogechukwu Eze, Margaret Cho, Antonio Galvao Neto, and Saul Harari
- Subjects
Pathology ,medicine.medical_specialty ,Proliferation index ,biology ,Adenoma ,medicine.diagnostic_test ,business.industry ,Rectosigmoid Colon ,Chromogranin A ,General Medicine ,medicine.disease ,digestive system diseases ,Dysplasia ,Tubulovillous adenoma ,Biopsy ,Synaptophysin ,biology.protein ,Medicine ,business - Abstract
High-grade neuroendocrine carcinomas (HGNEC) of the colon are rare. However, when they occur, there is evidence to support a strong association with tubulovillous/villous adenomas (TVA). We present a unique case of a colonic TVA associated with HGNEC in a 64-year-old woman with a large polypoid mass in the rectosigmoid colon diagnosed as TVA with high-grade dysplasia (HGD) on biopsy. Pathologic examination revealed a 4.8 cm friable polypoid mass composed of two morphologically distinct elements. The luminal portion of the tumor consisted of a TVA with areas of HGD. A distinct component was identified deeper to the adenoma, consisting of nested small-to-medium sized cells with increased nuclear-to-cytoplasmic ratio, nuclei exhibiting finely dispersed chromatin, inconspicuous nucleoli, patchy areas of necrosis, and increased mitotic figures. The two tumor components were closely apposed with focal areas of intermixing at their interface. Immunohistochemistry demonstrated diffuse positive staining of the deeper, nested neoplastic cells with synaptophysin, chromogranin, CD56 and CAM 5.2 and a perinuclear-dotlike pattern of pancytokeratin AE1/AE3 staining. The proliferation index was up to 90% (Ki-67). Given the overall findings, a diagnosis of HGNEC arising in a background of TVA was rendered. This unique case is one of the few reported cases of mixed adenoma HGNEC involving the colon. This case demonstrates possible pitfalls in diagnosis. Superficial biopsies often do not provide adequate representation of underlying neuroendocrine neoplasms. In this case, metastatic HGNEC was identified although initial biopsy revealed only TVA, suggesting that the two lesions were unrelated.
- Published
- 2014
10. Expression of VHL Causes Three-Dimensional Morphological Changes in Renal Cells Indicative of Proximal Tubule Differentiation
- Author
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Alan R. Schoenfeld, Ogechukwu Eze, and Shivannah S Chiatar
- Subjects
Gene isoform ,Tumor suppressor gene ,endocrine system diseases ,Cell ,General Medicine ,Biology ,Cell morphology ,medicine.disease_cause ,urologic and male genital diseases ,female genital diseases and pregnancy complications ,Article ,Ubiquitin ligase ,Cell biology ,medicine.anatomical_structure ,Immunology ,biology.protein ,medicine ,Carcinogenesis ,Developmental biology ,neoplasms ,Clear cell - Abstract
Mutations in the von Hippel-Lindau (VHL) tumor suppressor gene are responsible for the VHL hereditary cancer syndrome, and are associated with the majority of clear cell renal cell carcinomas. In this study, scanning electron microscopy of VHL-negative renal carcinoma cells was utilized to examine the effects of VHL re-expression on the morphology of these cells. Significant differences were observed between the morphology of VHL-negative control cells and those with reintroduced VHL, with VHL expression mediating an apical surface that mounded upward, as opposed to the flat surfaces seen with VHL-negative cells. In long term cultures, rounded VHL-expressing cells grew in clusters on top the monolayer, and microvilli were observed on the apical face of these cells, in a manner suggestive of proximal tubule differentiation. In contrast, VHL-negative cells remained flat and did not develop microvilli in long-term cultures. Since VHL is a key member of an ubiquitin E3 ligase complex whose best known target is hypoxia-inducible factor alpha (HIF-α), we looked at the effects of HIF-α expression on cell morphology. Knockdown of HIF-2α in cells that only express this isoform had no effect on the morphology of the cells. These results indicate that VHL expression directs three dimensional morphological changes in renal cells indicative of differentiation, and while dysregulation of HIF-α may be necessary for tumorigenesis following VHL loss, it is not the major determinant of these VHL-mediated morphological changes.
- Published
- 2013
11. Vanishing Thyroid Tumors: A Diagnostic Dilemma After Ultrasonography-Guided Fine-Needle Aspiration
- Author
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Julie Ann Sosa, Ashraf Khan, Lynwood Hammers, Robert Udelsman, Tobias Carling, Renu K. Virk, David Chhieng, Ogechukwu Eze, Manju L. Prasad, Guoping Cai, Constantine Theoharis, Sanziana A. Roman, and Zubair W. Baloch
- Subjects
Thyroid nodules ,Adult ,Male ,Pathology ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Biopsy ,Papillary ,Clinical Sciences ,Biopsy, Fine-Needle ,Thyroid Gland ,Thyroid Cancer ,Thyroid carcinoma ,Endocrinology & Metabolism ,Endocrinology ,Rare Diseases ,medicine ,Atypia ,Humans ,Thyroid Neoplasms ,Thyroid Nodule ,Thyroid cancer ,Cancer ,Aged ,Ultrasonography ,medicine.diagnostic_test ,business.industry ,Prevention ,Thyroid ,Carcinoma ,Thyroidectomy ,Nodule (medicine) ,Thyroid Cancer and Nodules ,Middle Aged ,medicine.disease ,Carcinoma, Papillary ,Brain Disorders ,Fine-needle aspiration ,medicine.anatomical_structure ,Thyroid Cancer, Papillary ,Fine-Needle ,Female ,medicine.symptom ,business - Abstract
BACKGROUND: Fine-needle aspiration (FNA) is the most accurate and cost-effective method for evaluating thyroid nodules. However, FNA-induced secondary changes completely replacing thyroid tumors (vanishing tumors) may create a novel problem. In this study, we highlight the diagnostic and management issues associated with the unintended consequences of ultrasonography (US)-guided FNA. METHODS: Fourteen thyroid glands (11 women and 3 men, ages 33–64 years) with vanishing tumors were prospectively identified between 2009 and 2012 upon surgical resection. Cytology and histopathology slides were reviewed, and second opinions were obtained when necessary. RESULTS: The cytology of the 14 vanishing tumors was suspicious/positive for papillary thyroid carcinoma (PTC) in 5, indeterminate (atypia of unknown significance) in 5, benign in 2, follicular neoplasm in 1, and nondiagnostic in 1 nodule. Upon thyroidectomy, the vanishing tumors ranged in size from 0.4 to 3.5 cm (median 0.7 cm). Microscopically, the nodules showed cystic degeneration, organizing hemorrhage, granulation tissue, fibrosis, and microcalcifications. In seven tumors, a few residual malignant cells (PTC in five) or residual benign follicles (hemorrhagic cyst in two) at the periphery of the vanishing tumors helped with the final diagnosis. The remaining seven tumors were completely replaced by FNA-induced secondary changes, and had the cytology diagnosis of benign in one, follicular neoplasm in one, and suspicious/positive for PTC in five. Of the latter five, two showed additional separate foci of PTC, while three vanishing tumors (0.5, 1.2, and 1.6 cm) had no residual malignant cells and no additional carcinoma leading to a final diagnosis of negative for malignancy. CONCLUSIONS: US-guided FNA may lead to complete obliteration of thyroid nodules, rendering final diagnosis upon thyroidectomy difficult or impossible. In these unusual circumstances, the possibility that the surgical pathology may be nonrepresentative should be considered if the cytologic features on FNA are sufficient by themselves to support a definitive diagnosis of PTC.
- Published
- 2013
12. A Brief Review of the Controversial Role of Iron in Colorectal Carcinogenesis
- Author
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Margaret Cho, Ogechukwu Eze, and Ruliang Xu
- Subjects
business.industry ,Colorectal cancer ,Cancer ,Colorectal carcinogenesis ,medicine.disease ,medicine.disease_cause ,Bioinformatics ,digestive system diseases ,Pathogenesis ,Red meat ,Medicine ,Refined grains ,business ,Carcinogenesis ,Male gender - Abstract
Colorectal Cancer (CRC) is the second and third most common cancer in females and males worldwide, respectively. Major risk factors have been established for the development of CRC. These include susceptible genetics, increased age (50 years), male gender, and race. The role of environmental factors in CRC pathogenesis is evident but complex. Some environmental factors linked to CRC pathogenesis include diet, specifically red meat, refined grains and starches, sugars, fat, alcohol, and chemicals. Implicated chemicals include arsenic, chromium, nickel and iron. This article briefly reviews the evidence for the controversial role of iron in pathogenesis of CRC. Proposed mechanisms for iron carcinogenesis involve various genetic and metabolic pathways, illustrating the complex interplay of genetic and metabolic alterations in chemical carcinogenesis
- Published
- 2013
13. Methylation profiling of locally advanced rectal cancer (LARC): Exploration of potential predictive markers for neoadjuvant chemoradiation (NACR)
- Author
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Cynthia A. Loomis, Adriana Heguy, Judith D. Goldberg, Jonathan Melamed, Lawrence Leichman, Debra J. Morrison, Theresa Ryan, Christopher Bowman, Harvey G. Moore, Sunjida Ahmed, Songchuan Guo, Matija Snuderl, Jonathan Serrano, Rachel I. Brody, Deirdre Jill Cohen, Cynthia G. Leichman, Ping Gu, Ogechukwu Eze, Jennifer Wu, and K.L. Du
- Subjects
Oncology ,Cancer Research ,medicine.medical_specialty ,Formalin fixed paraffin embedded ,business.industry ,Colorectal cancer ,Locally advanced ,Methylation ,University hospital ,Bioinformatics ,medicine.disease ,Total mesorectal excision ,Methylation profiling ,Internal medicine ,medicine ,business ,Laser capture microdissection - Abstract
614 Background: NACR followed by total mesorectal excision (TME) is standard of care for LARC. However, only 10-20% of patients achieve complete pathologic response with associated optimal clinical outcome. No predictive biomarker is currently available. Aberrant DNA methylation is well known for its key role in the pathogenesis of colorectal cancer. Previous rectal cancer methylation studies were conducted in patients without NACR or inconclusive. We hypothesized a correlation between methylation status and pathologic outcome following NACR. Methods: This is an IRB approved, retrospective, translational study of patients with LARC who received identical NACR before TME at New York University hospitals. Ten micron sections from FFPE tissue blocks underwent laser capture microdissection (LCM) followed by genome DNA isolation. Illumina Infinium 450k methylation array was used to assess 485,000 methylation sites. Methylation profiles and copy number changes were analyzed using in-house bioinformatics. Histone H3K27me, a global methylation marker, was assessed using immunohistochemistry (IHC) and expression quantified in carcinoma cells, normal epithelium, inflammatory cells, stromal cells and endothelial cells. Results: Tumor specimens from 52 patients were collected. Analyzed specimens of stromal and cancer cells showed distinct methylation profiles pre- and post- therapy. The histone H3K27me3 mark was markedly increased in carcinoma compared to normal epithelium and strong trimethyl mark expression persisted in invasive carcinoma after the therapy including single cells. We obtained adequate DNA to demonstrate differences between pre- and post- NACR specimens and tissue sites. Full data set, including the known clinical and pathologic results, is to be presented. Conclusions: This is the first study to combine LCM and high throughput quantitative methylation profiling in rectal cancer to correlate with NACR outcome. Histone H3K27me3 IHC can be used to identify high-grade and invasive carcinoma with high specificity. Data from our annotated clinical cohort and correlative molecular results can inform a future prospective trial.
- Published
- 2016
14. The role of epigenetic alterations in papillary thyroid carcinogenesis
- Author
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Tobias Carling, Ogechukwu Eze, and Lee F. Starker
- Subjects
Genetics ,lcsh:RC648-665 ,endocrine system diseases ,business.industry ,Endocrinology, Diabetes and Metabolism ,Review Article ,medicine.disease_cause ,lcsh:Diseases of the endocrine glands. Clinical endocrinology ,Gene expression profiling ,Thyroid carcinoma ,CpG site ,DNA methylation ,Gene silencing ,Medicine ,Epigenetics ,business ,Carcinogenesis ,Gene - Abstract
Papillary thyroid carcinoma (PTC) accounts for over 80% of all thyroid malignancies. The molecular pathogenesis remains incompletely clarified although activation of the RET fusion oncogenes, and RAS and BRAF oncogenes, has been well characterized. Novel technologies using genome-wide approaches to study tumor genomes and epigenomes have provided great insights into tumor development. Growing evidence shows that acquired epigenetic abnormalities participate with genetic alterations to cause altered patterns of gene expression/function. It has been established beyond doubt that promoter cytosine methylation in CpG islands, and the subsequent gene silencing, is intimately involved in cancer development. These epigenetic events very likely contribute to significant variation in gene expression profiling, phenotypic features, and biologic characteristics seen in PTC. Hypermethylation of promoter regions has also been analyzed in PTC, and most studies have focused on individual genes or a small cohort of genes implicated in tumorigenesis.
- Published
- 2011
15. The 2008 anatomy ceremony: voices, letter, poems
- Author
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Ogechukwu, Eze, Fiona, Horgan, Kim, Nguyen, Mona, Sadeghpour, and Alla Lescure, Smith
- Subjects
Students, Medical ,Dissection ,Poetry as Topic ,Arts & Humanities ,Anatomy ,Correspondence as Topic ,United States - Abstract
Yale University medical and PA students express their gratitude in a compilation of reflections on learning human anatomy.
- Published
- 2009
16. Molecular genetics of gastric adenocarcinoma in clinical practice
- Author
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Margaret Cho, Ogechukwu Eze, and Ruliang Xu
- Subjects
Oncology ,Clinical Practice ,medicine.medical_specialty ,Gastric adenocarcinoma ,business.industry ,Internal medicine ,Molecular genetics ,medicine ,business ,Gastroenterology - Published
- 2014
17. Wide circulation of peste des petits ruminants virus in sheep and goats across Nigeria
- Author
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Samuel E. Mantip, Anthony Sigismeau, Maurice Nanven, Atuman Joel, Abayomi M. Qasim, Sada Aliyu, Ibrahim Musa, Ogechukwu Ezeanyika, Ibikunle Faramade, Garba Ahmed, Timothy Y. Woma, David Shamaki, Genevieve Libeau, Souaibou Farougou, and Arnaud Bataille
- Subjects
peste des petits ruminants ,nigeria ,transboundary animal disease ,virus isolation ,rt-pcr ,morbillivirus ,sequencing ,Veterinary medicine ,SF600-1100 - Abstract
Peste des petits ruminants (PPR) is a highly contagious viral disease that mainly affects goats and sheep in Asia, Africa and the Middle East, and threatens Europe [R.E.1]. The disease is endemic on the African continent, particularly in West Africa, and is a major factor driving food insecurity in low-income populations. The aim of this research study was to carry out surveillance, genetic characterisation and isolation of recently circulating PPR viruses (PPRV) in sheep and goats from the six agro-ecological zones of Nigeria. A total of 268 post-mortem tissue samples of lung and mesenteric ganglia were collected from clinically suspected sheep and goats in 18 different states, of which five never previously sampled. The presence of PPRV was confirmed using a reverse-transcription coupled with a polymerase chain reaction (RT-PCR) assay. A total of 72 samples, 17 sheep (6%) and 55 goats (21%), were found to be PPR positive. Positive samples were distributed in almost all states, except Kano, where PPR was detected in previous studies. The PPRV-positive samples were further confirmed by sequencing or virus isolation in areas where the infection had never previously been detected. These results confirm the active circulation of PPRV across all six agro-ecological zones of Nigeria, and consequently, the need for introducing strict measures for the control and prevention of the disease in the country.
- Published
- 2021
- Full Text
- View/download PDF
18. Vanishing Thyroid Nodules: A Rare Diagnostic Dilemma Induced by Ultrasound-Guided Fine-Needle Aspiration
- Author
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Zubair W. Baloch, Robert Udelsman, Lynwood Hammers, Ogechukwu Eze, Manju L. Prasad, David C. Chhieng, Constantine Theoharis, Guoping Cai, and Ashraf Khan
- Subjects
Thyroid nodules ,medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,General Medicine ,Diagnostic dilemma ,medicine.disease ,Ultrasound guided ,Fine needle biopsy ,Fine-needle aspiration ,medicine ,Radiology ,Ultrasonography ,business - Published
- 2012
19. Braf-Associated Papillary Thyroid Cancer: Epigenetic Events Suggest Distinct Molecular Pathogenesis
- Author
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Tobias Carling, Ogechukwu Eze, William D. Long, P. Bjund, Lee F. Starker, and Robert Udelsman
- Subjects
Oncology ,medicine.medical_specialty ,business.industry ,Internal medicine ,medicine ,Molecular pathogenesis ,Cancer research ,Surgery ,Epigenetics ,medicine.disease ,business ,Papillary thyroid cancer - Published
- 2011
20. A rare case of Crohn's ileitis in a patient with constitutional mismatch repair deficiency.
- Author
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Kaimakliotis P, Giardiello F, Eze O, and Truta B
- Abstract
Constitutional mismatch repair deficiency (CMMRD), a variant of Lynch syndrome, is a rare disease characterized by café-au-lait spots, oligopolyposis, glioblastoma and lymphoma. A 24-year-old male, under surveillance for CMMRD, developed Crohn's ileitis after total colectomy with end ileostomy for colorectal cancer and failed to respond to oral corticosteroids. The patient underwent induction and maintenance of remission with vedolizumab infusions. We report the first patient with CMMRD developing Crohn's disease. The choice of immunosuppressive therapy in these patients is challenging and needs to be made according to their risk for malignancy., Competing Interests: Conflict of interest: None
- Published
- 2017
- Full Text
- View/download PDF
21. Clinical and biological significance of precursor lesions of intrahepatic cholangiocarcinoma.
- Author
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Ettel M, Eze O, and Xu R
- Abstract
Cholangiocarcinoma (CC) is primarily a malignant tumor of older adults most prevalent in Southeast Asia, where liver fluke infestation is high. However the etiology in western countries is unknown. Although the incidence of extrahepatic cholangiocarcinoma has remained constant, incidence of intrahepatic CC (ICC) which differs in morphology, pathogenesis, risk factors, treatment and prognosis is increasing. While this increase is associated with hepatitis C virus infection, chronic nonalcoholic liver disease, obesity, and smoking, the pathogenesis of ICC and molecular alterations underlying the carcinogenesis are not completely elucidated. Benign biliary lesions such as biliary intraepithelial neoplasia, intraductal papillary neoplasm of the bile duct, von Meyenburg complex or bile duct hamartoma, and bile duct adenoma have been associated with ICC. For each of these entities, evidence suggests or supports a role as premalignant lesions. This article summarized the important biological significance of the precursor lesions of ICC and the molecular mechanisms that may be involved in intrahepatic cholangiocarcinogenesis.
- Published
- 2015
- Full Text
- View/download PDF
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