Search

Nitrogen isotope analysis of chloropigments provides information on the sources of nitrogenous nutrients assimilated by phytoplankton. The abundant, ubiquitous chlorophyll a records nitrogen isotopic compositions (δ15N) of eukaryotic phytoplankton and cyanobacteria, whereas more source‐specific chloropigments, such as the divinylchlorophylls exclusively possessed by the marine picocyanobacterium Prochlorococcus, can potentially resolve isotopic variability within the phytoplankton community. In this study, we analyzed the δ15N of both chlorophyll a and divinylchlorophyll a isolated from suspended particulate material collected at the subsurface chlorophyll maximum (SCM) along a meridional transect at 88°E in the oligotrophic eastern Indian Ocean. The nitrogen isotopic compositions of Prochlorococcus (δ15NPRO) and the combined biomass of eukaryotic phytoplankton and Synechococcus (δ15NEU+SYN) estimated from the δ15N of divinylchlorophyll a and chlorophyll a, respectively, revealed systematic variations that were not apparent from bulk isotope measurements. Whereas consistently low δ15NPRO indicated reliance of Prochlorococcus on regenerated nitrogen throughout the transect, elevation in δ15NEU+SYN values at several stations was interpreted to reflect assimilation of subsurface NO3− by eukaryotic phytoplankton. The δ15N distributions revealed subtle differences in NO3− availability at the SCM along the transect, which were consistently explained by the occurrence of mesoscale eddies in the Bay of Bengal, deepening of the mixed layer induced by a seasonal Wyrtki Jet in the equatorial region, and substantial deepening of the nutricline in the South Indian Ocean gyre. Our results highlight the utility of compound‐specific isotopic measurements of multiple species of chlorophylls in obtaining essential non‐incubation‐based biogeochemical constraints on the primary production in the ocean. Key Points: We determined nitrogen sources for phytoplankton based on nitrogen isotopic measurements of chlorophyll a and divinylchlorophyll aProchlorococcus depended on regenerated nitrogen whereas eukaryotic phytoplankton responded sensitively to the subsurface nitrate supplyPhytoplankton responses were consistently explained by oceanographic conditions along a meridional transect in the eastern Indian Ocean [ABSTRACT FROM AUTHOR]

Periphytic algae attached to a streambed substrate (periphyton) are an important primary producer in stream ecosystems. We determined the isotopic composition of chlorophyll a in periphyton collected from a stream flowing on limestone bedrock in the Seri River, central Japan. Stable isotope ratios of carbon (δ13C) and nitrogen (δ15N) and natural radiocarbon abundances (Δ14C) were measured in chlorophyll a (δ13Cchl, δ15Nchl and Δ14Cchl) and bulk (δ13Cbulk, δ15Nbulk and Δ14Cbulk) for periphyton, a pure aquatic primary producer (Cladophora sp.) and a terrestrial primary producer (Quercus glauca). Periphyton δ13Cbulk and δ13Cchl values did not necessarily correspond to δ13Cbulk for an algal-grazing specialist (Epeorus latifolium). Periphyton Δ14Cchl values (-258 ‰ in April and -190 ‰ in October) were slightly lower than Δ14Cbulk values (-228 ‰ in April and -179 ‰ in October) but were close to the Δ14C value for dissolved inorganic carbon (DIC; -217 ± 31 ‰), which is a mixture of weathered carbonates (Δ14C = -1000 ‰), CO2 derived from aquatic and terrestrial organic matters (variable Δ14C) and dissolved atmospheric CO2 (Δ14C approximately +30 ‰ in 2013). Δ14Cchl values were also close to Δ14Cbulk for E. latifolium (-215 ‰ in April and -199 ‰ in October) and Cladophora sp. (-210 ‰), whereas the Δ14Cbulk value for Q. glauca (+27 ‰) was closer to Δ14C for atmospheric CO2. Although the bulk isotopic composition of periphyton is recognised as a surrogate for the photosynthetic algal community, natural periphyton is a mixture of aquatic and terrestrial organic materials. Our results indicate that the bulk periphyton matrix at the study site consists of 89 to 95% algal carbon (derived from 14C-depleted DIC) and 5 to 11% terrestrial organic carbon (derived from 14C-enriched atmospheric CO2). [ABSTRACT FROM AUTHOR]

Chloropigments and their derivative pheopigments preserved in sediments can directly be linked to photosynthesis. Their carbon and nitrogen stable isotopic compositions have been shown to be a good recorder of recent and past surface ocean environmental conditions tracing the carbon and nitrogen sources and dominant assimilation processes of the phytoplanktonic community. In this study we report results from combined compound-specific radiocarbon and stable carbon and nitrogen isotopic analysis to examine the timescales of synthesis and fate of chlorophyll-a and its degradation products pheophytin-a, pyropheophytin-a, and 13², 17³-cyclopheophorbide-a-enol until burial in Black Sea surface sediments. The pigments are mainly of marine phytoplanktonic origin as implied by their stable isotopic compositions. Pigment δ15N values indicate nitrate as the major uptake substrate but 15N-depletion towards the open marine setting indicates contribution from N2-fixation. Radiocarbon concentrations translate into minimum and maximum pigment ages of approximately 40 to 1200 years. This implies that protective mechanisms against decomposition such as association with minerals or eutrophication-induced hypoxia and light limitation are much more efficient than previously thought. However, seasonal variations of nutrient source, growth period, and habitat and their associated isotopic variability are likely at least as strong as long-term trends. Combined triple isotopic analysis of sedimentary chlorophyll and its primary derivatives is a powerful tool to delineate biogeochemical and diagenetic processes in the surface water and assess their precise timescales. [ABSTRACT FROM AUTHOR]

We determined the mean crustal uplifting rate during the late Holocene along the Soya Coast, Lützow-Holm Bay, East Antarctica, by dating a marine-lacustrine transition recorded in lake sediments. We focused on temporal variations in the chemical composition of sediments recovered from Lake Skallen Oike at Skallen and Lake Oyako at Skarvsnes. Both sets of lake sediments record environmental changes associated with a transition from marine to lacustrine (fresh water) settings, as indicated by analyses of sedimentary facies for carbon and nitrogen contents, nitrogen isotopic compositions (15N/14N), and major element concentrations. Changes in the dominant primary producers during the marine-lacustrine transition were also clearly revealed by biogenic Opal-A, diatom assemblages, and gradient gel electrophoresis (DGGE) with 16S rRNA gene analysis. Geochronology based on radiocarbon dating of acid-insoluble organic carbon suggested that the environmental transition from saline to fresh water occurred at 2940±100 cal yr BP at L. Skallen and 1060±90 cal yr BP at L. Oyako. Based on these data and a linear approximation model, we estimated a mean crustal uplifting rate of 3.6mmyr-1 for the period since the marine-lacustrine transition via brackish condition; this uplift is attributed to glacial-isostatic rebound along the Soya Coast. The geological setting was the primary factor in controlling the emergence event and the occurrence of simultaneous changes in sedimentary and biological facies along the zone of crustal uplift. [ABSTRACT FROM AUTHOR]