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Alcohol drinking is a leading risk factor for the development of esophageal squamous cell carcinoma (ESCC). However, the molecular mechanisms of alcohol-associated ESCC remain poorly understood. One of the most commonly mutated genes in ESCC is nuclear factor erythroid 2 like 2 (NFE2L2 or NRF2), which is a critical transcription factor regulating oxidative stress response and drug detoxification. When NRF2 is hyperactive in cancer cells, however, it leads to metabolic reprogramming, cell proliferation, chemoradioresistance, and poor prognosis. In this study, hyperactive NRF2 was found to up-regulate acetyl-CoA synthetase short-chain family members 2 (ACSS2), an enzyme that converts acetate to acetyl-CoA, in ESCC cells and mouse esophagus. We also showed that knockdown of NRF2 or ACSS2 led to decreased ACSS2 expression, which in turn reduced the levels of acetyl-CoA and ATP with or without ethanol exposure. In addition, ethanol exposure enhanced lipid synthesis in ESCC cells. Moreover, we observed a change in the metabolic profile of ESCC cells exposed to ethanol as a result of their NRF2 or ACSS2 status. We further showed that ACSS2 contributed to the invasive capability of NRF2high ESCC cells exposed to ethanol. In conclusion, the NRF2/ACSS2 axis mediates the metabolic effect of alcohol drinking on ESCC., (© 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.)

Public and nonprofit organizations are at the forefront of water scarcity issues using nonformal educational programs to drive behavior change towards water conservation. The efficacy of educational endeavors hinge on designing and delivering programs with considerations of the factors that will change relevant decisions and behaviors among residential landscape water users. The purpose of this study was to explore the concept of wellness and well-being, and its relationship to engagement in water conservation behaviors. To guide this evaluation, we integrated the theory of planned behavior with relevant tenets of cognitive and social psychology and included the psycho-social constructs of stress, perceived happiness and well-being. We conducted correlational and regression analyses to assess potential relationships. Overall, the results of the study show that each of the three different psycho-social measures behave differently with regards to current and future residential landscape water conservation behaviors. The models show that perceptions of well-being is the more consistent predictor for both current and future behaviors. Perceived happiness only served as a significant predictor for current behaviors. Stress simply demonstrated correlational relationships with current and future behaviors, not serving as a significant predictor. We suggest exploring the possibility of pairing and embedding educational programs focused on personal and family well-being with educational programs focused on environmental behavior. When we consider water conservation behavior as a function of more than knowledge and motivation, we can broaden our understanding of this behavior as a socially embedded experience., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Published by Elsevier Ltd.)

Kenya belongs to a high incidence region known as Africa's esophageal cancer (EC) corridor. It has one of the highest incidence rates of EC worldwide, but research on EC in Kenya has gone highly unnoticed. EC in Kenya is unique in its high percentage of young cases (< 30 years of age). In this review, we show the current status of EC in the country. We mainly focus on significant risk factors such as alcohol drinking, genetic factors, malnutrition and hot food/drink. Future directions in the study and prevention of EC in Kenya are also discussed., Competing Interests: Disclosure of conflict of interest None.

Background: Awareness of effects of long term use of computer and application of ergonomics in the computer workstation is important for preventing musculoskeletal disorders, eyestrain and psychosocial effects., Objectives: To determine the awareness of physical and psychological effects of prolonged computer usage and application of ergonomicsin the workstation., Design: One hundred and eighty one people were interviewed from tertiary educational institutions, telecommunications and media houses within Nairobi, Kenya., Subjects: Descriptive cross sectional study., Results: Majority (89.8%) of the respondents felt that prolonged computer use had an adverse effect on their health, with only 12.4% having received formal training on the same. Assessment of their workstations revealed the most applied ergonomic measure as feet placement on the floor: 100% (181) followed by correct monitor placement with 94.4% (171) fulfilling the requirements. The least applied ergonomic measures were non reflecting wall paint: 5% (9) and adjustable desk 9.9% (18)., Conclusion: There is awareness among computer users on the effects of prolonged computer use but there is limited application of ergonomic measures.

To assess the effect of the Expanded Program on Immunization (EPI) in rural Africa, blood samples were collected in two Kenyan sublocations. Serum antibodies against tetanus toxoid were measured in 155 individuals 1-70 years of age. Titers greater than the protective level of 0.01 IU/ml were found in 47% of the population. Protection was significantly higher in children born after the launching of the EPI (68%) and in women who had been at childbearing age since then (69%). Significantly lower protection was demonstrated in other age and sex-groups. The level of protection in children was equal in the two populations, whereas protection in fertile women was significantly lower in the population living a long distance from a health center. Diphtheria anti-toxin was measured in the samples from one sublocation, and 70 of 84 individuals (83%) had antibody levels greater than the protective level. No age or sex difference could be found, and there was no correlation between response levels to diphtheria and tetanus. This implicates natural infections as an important source of diphtheria antibodies. Our findings demonstrate a need for better coverage of the adult population against tetanus. Furthermore, diphtheria transmission still appears to take place, underscoring the importance of diphtheria vaccination of travelers to rural Africa.

Visceral and cutaneous Leishmaniasis have been widely recognised as in vitro models for the differentia! regulation of CD4+ T cell subsets. These subsets are characterised on the basis of partially non-overlapping patterns of cytokine production. Thus, Th l type cells produce IL-2 and IFN y, whereas Th 2 cells produce IL-4 and IL-5. Both cell types are capable of producing other cytokines such as IL-3. The relationship between the functional expression of these populations and disease outcome has become a central focus for vaccine design, with potentially protective antigen formulations being screened for their ability to specifically induce cytokines, characteristic of the host protective reponse. Observations such as these are important in designing therapy and vaccines for most infectious diseases.

An enzyme-linked immunosorbent assay (ELISA) using native gp63 for detection of serum antibodies to Leishmania was evaluated. The test identified antibodies in sera from 16 of 16 visceral leishmaniasis (VL) patients and 9 of 12 sera from patients with Trypanosoma brucei infection. In comparison, sera from 80 Danish controls and 40 control donors from a malaria endemic area of Ghana without known exposure to Leishmania were negative, as were sera from 12 Kenyan malaria patients and 9 schistosomiasis patients. After cure of VL, sera rapidly became negative. Only 1 of 7, 1 of 21, and 1 of 27 sera from cured VL patients 6-12 months, 1-2 years and > 2 years after cure were positive. Thus, in contrast to other serological tests for VL, the gp63 ELISA seems to distinguish an ongoing from a past infection. This might prove useful both for diagnostic and epidemiological purposes.

Two soluble antigens from Leishmania donovani of 116 kDa and 70 kDa molecular mass, and a soluble mixture of crude antigens, were used in an enzyme-linked immunosorbent assay (ELISA) for the detection of visceral leishmaniasis (VL) in the field, and compared with the direct agglutination test (DAT). The tests were carried out on 8 VL patients, 34 normal individuals from an area endemic for the disease, and 68 former visceral leishmaniasis patients 1-5 years after treatment. The 70 kDa ELISA and the DAT had a sensitivity and specificity of 100% (95% confidence interval 63-100%), while the 116 kDa ELISA and the soluble crude antigen ELISA were 37.5% (9-76%) and 50% (16-84%) sensitive, respectively. When using ELISA (116 kDa or 70 kDa), 68-69% of sera tested 1-2 years, and 92-94% of sera tested 5 years, after treatment were negative. In contrast, when DAT or ELISA with crude antigen were used, the negativity rate was 31% 1-2 years, and 53% 5 years, after treatment. DAT was therefore not an accurate test for diagnosis in the field. The use of the 70 kDa antigen in ELISA was an accurate alternative to DAT in the detection of VL.

In a cross-sectional house-to-house study in a leishmaniasis-endemic area in Kenya, the cellular and humoral immune response to Leishmania lipophosphoglycan (LPG) was determined. Clinical data, peripheral blood mononuclear cells, and plasma were obtained from 50 individuals over the age of eight years. Lymphoproliferation and interferon-gamma (IFN-gamma) production by these cells were examined. It was shown that cells from all six individuals in the population with a history of kala-azar responded to LPG in the lymphocyte proliferation assay, and four of these six responded in the IFN-gamma assay. In contrast, cells from 12 of 44 individuals from the study area with no history of kala-azar and none of the five Danish control samples responded to LPG. Antibodies against LPG were detected by enzyme-linked immunosorbent assay in 45 of 50 plasma samples. Our findings clearly show that mononuclear cells from kala-azar patients cured of infection were able to respond to the LPG preparation. The finding of a specific cellular immune response to LPG in 12 of 44 individuals with no history of kala-azar is consistent with previous epidemiologic studies, in which it has been shown that a proportion of L. donovani infections run a subclinical course. The high frequency of individuals with antibodies against LPG might indicate that a majority of the population had been exposed to the parasite.(ABSTRACT TRUNCATED AT 250 WORDS)