26 results on '"Ochoa JC"'
Search Results
2. Aplicación del método de Graf para el diagnóstico y tratamiento oportuno de displasia de cadera
- Author
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Villanueva-Martínez, S, primary, Hermida-Ochoa, EH, additional, Benavides-Rodríguez, D, additional, and Hermida-Ochoa, JC, additional
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- 2022
- Full Text
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3. Neuromodulación percutánea utilizando radiofrecuencia pulsada versus crioablación en pacientes con síndrome facetario lumbar
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Hermida-Ochoa, JC, primary, Benavides-Rodríguez, D, additional, Hermida-Ochoa, EH, additional, and González-Ramos, LF, additional
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- 2022
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4. Tratamiento de deformidad severa de la columna vertebral con modelos de impresión 3D
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Evia-Cabral, RG, primary, Hermida-Ochoa, EH, additional, Benavides-Rodríguez, D, additional, Cuevas-Andrade, JL, additional, Uribe-Cortés, TB, additional, and Hermida-Ochoa, JC, additional
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- 2022
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5. Análisis biomecánico de la ligamentoplastía interespinosa con fibra de poliéster posterior a discectomía
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Martínez-López, L, primary, Hermida-Ochoa, JC, additional, Hermida-Ochoa, EH, additional, Cuevas-Andrade, JL, additional, and Benavides-Rodríguez, D, additional
- Published
- 2021
- Full Text
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6. Immune-serological identification of infectious agents with influence on bovine embryo transfer in the north of Brazil
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Diniz, JV, Ochoa, JC, Montoya, LM, Satrapa, R, Okuda, LH, Pituco, EM, Marcelino, RR, and Oba, E
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reproductive ,embrión ,cattle ,receptor ,ganado ,embryo ,reproductivo ,recipients - Abstract
The aim of this study was to investigate antibodies against brucellosis, leptospirosis, bovine rhinotracheitis (IBR/ BoHV-1), bovine viral diarrhea (BVD) and neosporosis through immunodiagnostic. These agents may directly or indirectly compromise the female bovine reproductive tract as well as embryonic and fetal development, therefore we also tried to establish a probable relation between those antibody with the utilization rates of fixed-time embryo transfer (FTET) protocols, with pregnancy rate per embryo transfer (ET) and the occurrence of miscarriages. Embryos were obtained by in vitro fertilization of the ovum and sperm from the Gyr dairy breed, which were transferred to 235 zebu-crossbred cows under FTET protocols. The blood samples to obtain serum were collected by venous puncture from coccygeal vein, only a few days of the completion of ET. For the immunodiagnostic research of brucellosis, leptospirosis, IBR/BoHV-1, BVD and neosporosis, the complement fixation assay, the microscopic agglutination test, virus neutralization, and linked immunosorbent assay to enzymes in conjunction with the indirect immunofluorescence were used. The utilization rates of the FTET protocols related to pregnancy and abortion in D16, D35, and D75 were 67.23%, 34,18% and 20,37% respectively. The immunodiagnostic results related to seropositive animals were obtained for brucellosis (7/235 = 2.89%), leptospirosis (128/235 = 54.47%), IBR (103/235 = 43.83%), BVD (174/235 = 74.04%), and neosporosis (150/235 = 63.82%). In seropositive recipients, there was an observed influence (P < 0.05) of leptospirosis and neosporosis on the rate of protocol utilization of brucellosis on both the pregnancy and abortion rate, and of BVD and IBR on abortion rate. El estudio tuvo por finalidad evidenciar, mediante técnicas de inmunodiagnóstico, anticuerpos contra Brucelosis, Leptospirosis, Rinotraqueítis Infecciosa Bovina (IBR), Diarrea Viral Bovina (DVB) y Neosporosis, agentes que directa o indirectamente pueden comprometer el tracto reproductor de las hembras así como el desarrollo embrionario y fetal. Se estableció una probable relación entre la presencia de anticuerpos contra los agentes con la tasa de aprovechamiento de protocolos de transferencias de embriones en tiempo fijo (TETF), la tasa de preñez por transferencia de embriones (TE) y la ocurrencia de abortos. Los embriones fueron obtenidos por fertilización in vitro (FIV) a partir de aspiración folicular y semen de toros, ambos de raza Gyr lechero, y tranferidos a 235 vacas receptoras cruzadas con Cebú sometidas a protocolos para TETF. Se obtuvo suero de muestras de sangre mediante venopunción de la vena coccígea solamente en los días de realización de TE. Para evidenciar Brucelosis, Leptospirosis, IBR, DVB y Neospora se emplearon las técnicas de fijación de complemento, seroaglutinación microscópica, seroneutralización y test de inmunoensayo (ELISA) junto con la inmunofluorescencia indirecta (RIFI), respectivamente. El diagnóstico de gestación de las receptoras fue efectuado por ultrasonografía, a los 35 días después de la TE y repetido a los 75 días para confirmar la gestación o eventual aborto. Los animales serorreactivos para cada agente fueron: Brucella (7/235 = 2,89%), Leptospirosis (128/235 = 54,47%), IBR (98/235 = 43,83%), DVB (174/235 = 74,04%) y Neosporosis (150/235 = 63,82%). En las receptoras serorreactivas se observaron influencias (P < 0,05) de la leptospirosis y de la neosporosis sobre la tasa de aprovechamiento del protocolo, de brucelosis en las tasas de preñez y aborto y en DVB e IBR en las tasas de aborto.
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- 2016
7. Immune-serological identification of infectious agents with influence on bovine embryo transfer in the north of Brazil
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Diniz, JV, primary, Ochoa, JC, additional, Montoya, LM, additional, Satrapa, R, additional, Okuda, LH, additional, Pituco, EM, additional, Marcelino, RR, additional, and Oba, E, additional
- Published
- 2016
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8. Natural variation in Arabidopsis responses to Plasmodiophora brassicae reveals an essential role for Resistance to Plasmodiophora brasssicae 1 (RPB1).
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Ochoa JC, Mukhopadhyay S, Bieluszewski T, Jędryczka M, Malinowski R, and Truman W
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- Plant Diseases, Genome-Wide Association Study, Arabidopsis metabolism, Plasmodiophorida, Brassica genetics
- Abstract
Despite the identification of clubroot resistance genes in various Brassica crops our understanding of the genetic basis of immunity to Plasmodiophora brassicae infection in the model plant Arabidopsis thaliana remains limited. To address this issue, we performed a screen of 142 natural accessions and identified 11 clubroot-resistant Arabidopsis lines. Genome-wide association analysis identified several genetic loci significantly linked with resistance. Three genes from two of these loci were targeted for deletion by CRISPR/Cas9 mutation in resistant accessions Est-1 and Uod-1. Deletion of Resistance to Plasmodiophora brassicae 1 (RPB1) rendered both lines susceptible to the P. brassicae pathotype P1+. Further analysis of rpb1 knock-out Est-1 and Uod-1 lines showed that the RPB1 protein is required for activation of downstream defence responses, such as the expression of phytoalexin biosynthesis gene CYP71A13. RPB1 has recently been shown to encode a cation channel localised in the endoplasmic reticulum. The clubroot susceptible Arabidopsis accession Col-0 lacks a functional RPB1 gene; when Col-0 is transformed with RPB1 expression driven by its native promoter it is capable of activating RPB1 transcription in response to infection, but this is not sufficient to confer resistance. Transient expression of RPB1 in Nicotiana tabacum induced programmed cell death in leaves. We conclude that RPB1 is a critical component of the defence response to P. brassicae infection in Arabidopsis, acting downstream of pathogen recognition but required for the elaboration of effective resistance., (© 2023 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.)
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- 2023
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9. RXam2, a NLR from cassava (Manihot esculenta) contributes partially to the quantitative resistance to Xanthomonas phaseoli pv. manihotis.
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Díaz-Tatis PA, Ochoa JC, Rico EM, Rodríguez C, Medina A, Szurek B, Chavarriaga P, and López CE
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- Leucine, Nucleotides, Plant Diseases microbiology, Manihot genetics, Xanthomonas
- Abstract
Key Message: The overexpression of RXam2, a cassava NLR (nucleotide-binding leucine-rich repeat) gene, by stable transformation and gene expression induction mediated by dTALEs, reduce cassava bacterial blight symptoms. Cassava (Manihot esculenta) is a tropical root crop affected by different pathogens including Xanthomonas phaseoli pv. manihotis (Xpm), the causal agent of cassava bacterial blight (CBB). Previous studies have reported resistance to CBB as a quantitative and polygenic character. This study sought to validate the functional role of a NLR (nucleotide-binding leucine-rich repeat) associated with a QTL to Xpm strain CIO151 called RXam2. Transgenic cassava plants overexpressing RXam2 were generated and analyzed. Plants overexpressing RXam2 showed a reduction in bacterial growth to Xpm strains CIO151, 232 and 226. In addition, designer TALEs (dTALEs) were developed to specifically bind to the RXam2 promoter region. The Xpm strain transformed with dTALEs allowed the induction of the RXam2 gene expression after inoculation in cassava plants and was associated with a diminution in CBB symptoms. These findings suggest that RXam2 contributes to the understanding of the molecular basis of quantitative disease resistance., (© 2021. The Author(s), under exclusive licence to Springer Nature B.V.)
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- 2022
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10. [Percutaneous neuromodulation with pulsating radiofrequency vs cryoablation for the treatment of patients with lumbar facet syndrome].
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González-Ramos LF, Hermida-Ochoa EH, Benavides-Rodríguez D, and Hermida-Ochoa JC
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- Humans, Pain Measurement, Physical Examination, Cryosurgery, Low Back Pain surgery
- Abstract
Introduction: lumbar spine pain affects a high percentage of the population and has a significant socioeconomic impact. Lumbar facet syndrome has a prevalence between 15-31% with lifetime incidence of up to 52% in some series. Due to the use of different types of treatment and different patient selection criteria, the success rate varies in the literature., Objective: to compare results of treatment with rhizolysis applying pulsed radiofrequency versus cryoablation in patients diagnosed with lumbar facet syndrome., Material and Methods: from January 2019 to November 2019, eight patients were randomly divided into two groups: group A treated with pulsed radiofrequency and group B treated with cryoablation. Pain was assessed with the visual analogue scale and the Oswestry low back pain disability index at four weeks, in addition to three and six months., Results: follow-up was six months. Immediately the eight patients (100%) reported improvement in symptoms and pain. Of the four patients who were in intense functional limitation, one of them became without functional limitation, and two of them went to minimum functional limitation and one to moderate functional limitation at the first month, statistically significant differences were reported., Conclusions: both treatments control pain in the short term; there is also an improvement in physical abilities. The morbidity accompanying neurolysis either radiofrequency or cryoablation is very low.
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- 2022
11. [Application of the Graf method for diagnosis and early detection of hip dysplasia].
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Villanueva-Martínez S, Hermida-Ochoa EH, Benavides-Rodríguez D, and Hermida-Ochoa JC
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- Child, Cross-Sectional Studies, Early Diagnosis, Female, Humans, Infant, Infant, Newborn, Pregnancy, Retrospective Studies, Hip Dislocation, Hip Dislocation, Congenital diagnostic imaging, Hip Dislocation, Congenital therapy
- Abstract
Introduction: Developmental hip dysplasia (DHD) is the most common disorder affecting pediatric hip; screening all neonates clinically, and using ultrasonography selectively for those babies who are at high risk is a widespread recommendation. our goal is to evaluate the impact that USG diagnosis and early treatment of DHD has had on the child population of our unit., Material and Methods: Retrospective, descriptive and cross-sectional study. Records of those children from one to six months of age, with a diagnosis of DHD, without distinction of sex, subjected to ultrasonographic tracking in the period from January 2018 to December 2019 were reviewed. A follow-up of six months was carried out in all patients, from the moment of diagnosis and the start of treatment with harness, weekly visits for relocation, as well as ultrasonographic revision every four weeks to monitor the treatment., Results: 19 cases were reported from the left side (47.5%), 10 cases from the right side (25%) and 11 bilateral cases (27.5%). The main associated risk factors were: product of the first pregnancy, family history of DHD, pelvic presentation, female sex. The results were favorable with a continuous use of harness of 23 hours observing a satisfactory evolution in 99.2% of the patients., Conclusion: With the results obtained we can analyze the considerable success rate of the hip clinic of our hospital with the realization of the ultrasound, we find a lower incidence of patients with pain, limitation of function, as well as satisfactory gait patterns.
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- 2022
12. [Treatment of severe spinal deformity with 3D printing models].
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Evia-Cabral RG, Hermida-Ochoa EH, Benavides-Rodríguez D, Cuevas-Andrade JL, Uribe-Cortés TB, and Hermida-Ochoa JC
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- Adolescent, Female, Humans, Male, Operative Time, Printing, Three-Dimensional, Scoliosis surgery, Spinal Fusion methods, Surgery, Computer-Assisted
- Abstract
Introduction: Scoliosis is a complex deformity that affects all three planes of the axis of the spine. The association between neuromuscular pathology and vertebral alignment was initially described in 1960. Neuromuscular pathology is progressive and results in postural abnormalities. Surgical goals in patients with neuromuscular deformity include anatomical correction for sedation and ambulation, as well as functional improvement. The gold standard of treatment is by posterior approach with transpedicular screws. The "hands-free" technique saves surgical time, decreases radiation by reducing the use of fluoroscopy. The advent of 3D printing technology allows precise study of the anatomical area and detail of the deformity in its three planes. This model can be sterilized for transoperative guidance. A 13-year-old female patient who develops thoracolumbar neuromuscular scoliosis secondary to spastic cerebral palsy (CP), with previous instrumentation T11-L3 of which he develops severe proximal curve. After the segmentation of the three-dimensional model, pedicle violation greater than 2 mm towards bilateral medullary canal was detected in the pedicles of L1 and L2 of previous instrumentation, pedicle dysplasia and the morphological characteristics of the pedicles were observed. Three-dimensional planning and the use of surgical guides represent a tool for surgical planning, especially in severe cases and with pedicle dysplasia. It helps as a surgical guide for the placement of hands-free transpedicular screws with possible reduction of radiation and anesthetic time.
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- 2022
13. Tackling equitable coverage and quality of care for neonates in hospitals: a pre-post assessment on asphyxia interventions in Mesoamerica.
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Kamath AM, Thom MG, Johanns CK, Panhorst Harris K, Schwarzbauer K, Ochoa JC, Zuniga-Brenes P, Rios-Zertuche D, Mokdad AH, and Hernandez B
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- Child, Delivery of Health Care, Health Facilities, Hospitals, Humans, Infant, Newborn, Quality of Health Care, Asphyxia, Asphyxia Neonatorum therapy
- Abstract
Background: Intrapartum-related hypoxic events, or birth asphyxia, causes one-fourth of neonatal deaths globally and in Mesoamerica. Multidimensional care for asphyxia must be implemented to ensure timely and effective care of newborns. Salud Mesoamérica Initiative (SMI) is a performance-based program seeking to improve maternal and child health for low-income areas of Central America. Our objective was to assess the impact of SMI on neonatal asphyxia care in health centers and hospitals in the region., Methods: A pre-post design. Two hundred forty-eight cases of asphyxia were randomly selected from medical records at baseline (2011-2013) and at second-phase follow-up (2017-2018) in Mexico (state of Chiapas), Honduras, Nicaragua, and Guatemala as part of the SMI Initiative evaluation. A facility survey was conducted to assess quality of health care and the management of asphyxia. The primary outcome was coverage of multidimensional care for the management of asphyxia, consisting of a skilled provider presence at birth, immediate assessment, initial stabilization, and appropriate resuscitation measures of the newborn. Data were analyzed using multivariable logistic regression., Results: Management of asphyxia improved significantly after SMI. Proper care of asphyxia in intervention areas was better (OR = 2.4; 95% CI = 1.3-4.6) compared to baseline. Additionally, multidimensional care was significantly higher in Honduras (OR = 4.0; 95% CI = 1.4-12.0) than in Mexico. Of the four multidimensional care components, resuscitation showed the greatest progress by follow-up (65.7%) compared to baseline (38.7%)., Conclusion: SMI improved the care for neonatal asphyxia management across all levels of health care in all countries. Our findings show that proper training and adequate supplies can improve health outcomes in low-income communities. SMI provides a model for improving health care in other settings., (© 2021. The Author(s).)
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- 2021
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14. Functionality of Bread and Beverage Added with Brosimum alicastrum Sw. Seed Flour on the Nutritional and Health Status of the Elderly.
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Rodríguez-Tadeo A, Del Hierro-Ochoa JC, Moreno-Escamilla JO, Rodrigo-García J, de la Rosa LA, Alvarez-Parrilla E, López-Díaz JA, Vidaña-Gaytán ME, González-Valles MN, Larqué-Saavedra A, and Martínez-Ruiz NDR
- Abstract
Physiological changes in elderly individuals (EI) can contribute to nutritional deterioration and comorbidities that reduce their quality of life. Factors such as diet can modulate some of these effects. The aim was to evaluate the functionality of foods added with Brosimum alicastrum Sw. seed flour in EI. EI ( n = 23) living in nursing home conditions agreed to participate. A control stage was carried out (30 days) and subsequently, an intervention stage (30 days) was realized in which a muffin and a beverage, designed for EI, were added to the participants' their usual diet. In both stages, anthropometric parameters, body composition, nutritional status, dietary intake, sarcopenic status, cognitive and affective states, biometric parameters, and total phenolic compounds (TPC), and antioxidant capacity in foods and plasma of EI were determined. The results showed that the consumption of the foods improved the energy intake and preserved the muscle reserves of the EI. The EI gained body weight (+1.1 kg), increased their protein (+18.6 g/day; 1.5 g/kg BW/day), dietary fiber (+13.4 g/day), iron (+4.4 mg/day), zinc (+1.8 mg/day), folic acid (+83.4 µg/day) consumption while reducing their cholesterol (-66 mg/day) and sodium (-319.5 mg/day) consumption. LDL-C lipoproteins reduced (14.8%) and urea (33.1%) and BUN (33.3%) increased. The TPC increased (7.8%) in the plasma, particularly in women (10.7%). The foods improve the EI nutritional status, and this has a cardiovascular protective effect that can benefit the health of the EI.
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- 2021
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15. [Biomechanical analysis of interespinous ligamentoplasty with polyester fiber after discectomy].
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Martínez-López L, Hermida-Ochoa JC, Hermida-Ochoa EH, Cuevas-Andrade JL, and Benavides-Rodríguez D
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- Animals, Biomechanical Phenomena, Lumbar Vertebrae surgery, Range of Motion, Articular, Swine, Diskectomy, Polyesters
- Abstract
Introduction: There are different pathologies of the lumbar spine that condition a biomechanical and clinical instability for its treatment, various stabilization techniques have been carried out that try to preserve the movement and the transmission of load of the affected segment such as the interspinatus ligamentplasty with Dallos® fiber., Objective: To show the biomechanical variations of functional segment of lumbar spine of pigs, before and after performing the discectomy and ligamentoplasty with Dallos® fiber., Material and Methods: The lumbar segment was mounted in a servo-hydraulic multiaxial simulator. Mobility ranges of flexion, extension, lateral flexion and axial rotations were simulated under three conditions: 1. Natural segment, 2. Discectomy segment, and 3. Disectomized segment plus ligamentoplasty with Dallos® fiber. The mobility ranges are made up to a torque of 7.5 N-m The data of the torques and mobility ranges was collected in the simulator program and the results of the biomechanical changes between the three conditions described were plotted., Results: It was shown that lumbar biomechanics is affected after discectomy mainly in flexion and extension. In the left axial bending and rotation movements, an alteration of torque and mobility ranges was found., Conclusions: The ligamentoplasty recovers part of the stability lost after discectomy preserving part of the disc height without reaching to equalize the movements as in the natural segment. After discectomy the distribution of force suggests that residual instability with ligament plasty may represent facet overload.
- Published
- 2021
16. Comparison of immediate colposcopy, repeat conventional cytology and hrHPV testing for the clinical management of ASC-US cytology in routine health services of Medellin, Colombia: The ASCUS-COL Trial.
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Baena A, Agudelo MC, Lopez C, Ramírez AT, Castañeda KM, Bedoya AM, Riveros M, Posada G, Borrero M, Buitrago CA, Suescun D, Gomez LJ, Ochoa JC, Stoler M, Gage J, Castle PE, Sasieni P, Almonte M, Herrero R, and Sanchez GI
- Abstract
In the context of opportunistic cervical cancer screening settings of low-and-middle-income countries, little is known about the benefits of high-risk human papillomavirus (hrHPV) testing on high-grade cervical abnormality detection among women with atypical squamous cells of undetermined significance (ASC-US) cytology in routine clinical practice. We compared the effectiveness of immediate colposcopy (IC), conventional cytology at 6 and 12 months (colposcopy if ≥ASC-US) (RC), and hrHPV testing (colposcopy if hrHPV-positive) (HPV) to detect cervical intraepithelial neoplasia grade 2 or more severe diagnoses (CIN2+) among women aged 20-69 years with ASC-US in routine care. Participants (n=2,661) were evenly randomized into three arms (n=882 IC, n=890 RC, n=889 HPV) to receive services by routine healthcare providers and invited to an exit visit 24 months after recruitment. Histopathology was blindly reviewed by a quality-control external panel (QC). The primary endpoint was the first QC-diagnosed CIN2+ or CIN3+ detected during three periods: enrolment (≤6 months for IC and HPV, ≤12 months for RC), follow-up (between enrolment and exit visit), and exit visit. The trial is completed. Colposcopy was done on 88%, 42%, and 52% of participants in IC, RC, and HPV. Overall, 212 CIN2+ and 52 CIN3+ cases were diagnosed. No differences were observed for CIN2+ detection (p=0.821). However, compared to IC, only HPV significantly reduced CIN3+ cases that providers were unable to detect during the 2-year routine follow-up (relative proportion 0.35, 95% CI 0.09-0.87). In this context, hrHPV testing was the most effective and efficient management strategy for women with ASC-US cytology., (This article is protected by copyright. All rights reserved.)
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- 2020
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17. Short communication: Heat stress does not affect induced luteolysis in Holstein cows.
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Mogollón HDG, Ferrazza RA, Vallejo VH, Destro FC, Ochoa JC, Nogueira C, Carvalho RF, Moraes LN, Rizzoto G, Sartori R, Wiltbank MC, and Ferreira JCP
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- Animals, Estrous Cycle drug effects, Female, Hot Temperature, Oxytocics pharmacology, Progesterone pharmacology, Cattle physiology, Corpus Luteum drug effects, Dinoprost pharmacology, Heat-Shock Response, Luteolysis drug effects
- Abstract
Heat stress (HS) has deleterious effects on bovine reproduction, including prolongation of the luteal phase in Holstein cows, perhaps due to compromised luteolysis. The objective was to characterize effects of HS on luteolytic responses of nonlactating Holstein cows given 25 or 12.5 mg of PGF
2α on d 7 of the estrous cycle. Cows were randomly distributed into 2 environments: thermoneutral (n = 12; 25°C) or HS (n = 12; 36°C). In each environment, cows were treated with 2 mL of saline, 25 or 12.5 mg of PGF2α (n = 4 cows per group). The HS environment induced a significant increase in rectal temperature and respiratory rate compared with the thermoneutral environment. Heat stress did not have significant effects on luteolytic responses or circulating progesterone concentrations. Rapid and complete luteolysis occurred in all cows given 25 mg of PGF2α and in 4 of 8 cows given 12.5 mg; the other 4 cows given 12.5 mg had partial luteolysis, with circulating progesterone concentrations initially suppressed, but subsequently rebounding. Therefore, we conclude that HS does not change corpus luteum sensitivity to PGF2α ., (Copyright © 2020 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)- Published
- 2020
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18. Visualization of Phytophthora palmivora Infection in Oil Palm Leaflets with Fluorescent Proteins and Cell Viability Markers.
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Ochoa JC, Herrera M, Navia M, and Romero HM
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Bud rot (BR) is the most devastating disease affecting oil palm ( Elaeis guineensis ) crops in Colombia. Its causal agent, Phytophthora palmivora , initiates the infection in immature oil palm leaflets producing necrotic lesions, followed by colonization of opportunistic necrotrophs, which increases disease damage. To improve the characterization of the disease, we transformed P. palmivora using Agrobacterium tumefaciens -mediated transformation (ATMT) to include the fluorescent proteins CFP-SKL (peroxisomal localization), eGFP and mRFP1 (cytoplasmic localization). The stability of some transformants was confirmed by Southern blot analysis and single zoospore cultures; additionally, virulence and in vitro growth were compared to the wild-type isolate to select transformants with the greatest resemblance to the WT isolate. GFP-tagged P. palmivora was useful to identify all of the infective structures that are commonly formed by hemibiotrophic oomycetes, including apoplastic colonization and haustorium formation. Finally, we detected cell death responses associated with immature oil palm tissues that showed reduced susceptibility to P. palmivora infection, indicating that these tissues could exhibit age-related resistance. The aim of this research is to improve the characterization of the initial disease stages and generate cell biology tools that may be useful for developing methodologies for early identification of oil palm materials resistant or susceptible to BR.
- Published
- 2019
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19. Mechanisms for rescue of corpus luteum during pregnancy: gene expression in bovine corpus luteum following intrauterine pulses of prostaglandins E1 and F2α.
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Ochoa JC, Peñagaricano F, Baez GM, Melo LF, Motta JCL, Garcia-Guerra A, Meidan R, Pinheiro Ferreira JC, Sartori R, and Wiltbank MC
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- Animals, Cattle, Corpus Luteum metabolism, Female, Luteolysis drug effects, Pregnancy, Progesterone blood, Uterus metabolism, Alprostadil pharmacology, Corpus Luteum drug effects, Dinoprost pharmacology, Gene Expression drug effects, Uterus drug effects
- Abstract
In ruminants, uterine pulses of prostaglandin (PG) F2α characterize luteolysis, while increased PGE2/PGE1 distinguish early pregnancy. This study evaluated intrauterine (IU) infusions of PGF2α and PGE1 pulses on corpus luteum (CL) function and gene expression. Cows on day 10 of estrous cycle received 4 IU infusions (every 6 h; n = 5/treatment) of saline, PGE1 (2 mg PGE1), PGF2α (0.25 mg PGF2α), or PGE1 + PGF2α. A luteal biopsy was collected at 30 min after third infusion for determination of gene expression by RNA-Seq. As expected, IU pulses of PGF2α decreased (P < 0.01) P4 luteal volume. However, there were no differences in circulating P4 or luteal volume between saline, PGE1, and PGE1 + PGF2α, indicating inhibition of PGF2α-induced luteolysis by IU pulses of PGE1. After third pulse of PGF2α, luteal expression of 955 genes were altered (false discovery rate [FDR] < 0.01), representing both typical and novel luteolytic transcriptomic changes. Surprisingly, after third pulse of PGE1 or PGE1 + PGF2α, there were no significant changes in luteal gene expression (FDR > 0.10) compared to saline cows. Increased circulating concentrations of the metabolite of PGF2α (PGFM; after PGF2α and PGE1 + PGF2α) and the metabolite PGE (PGEM; after PGE1 and PGE1 + PGF2α) demonstrated that PGF2α and PGE1 are entering bloodstream after IU infusions. Thus, IU pulses of PGF2α and PGE1 allow determination of changes in luteal gene expression that could be relevant to understanding luteolysis and pregnancy. Unexpectedly, by third pulse of PGE1, there is complete blockade of either PGF2α transport to the CL or PGF2α action by PGE1 resulting in complete inhibition of transcriptomic changes following IU PGF2α pulses.
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- 2018
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20. Pivotal periods for pregnancy loss during the first trimester of gestation in lactating dairy cows.
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Wiltbank MC, Baez GM, Garcia-Guerra A, Toledo MZ, Monteiro PL, Melo LF, Ochoa JC, Santos JE, and Sartori R
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- Animals, Cattle, Embryo Loss veterinary, Female, Lactation physiology, Pregnancy, Abortion, Veterinary etiology, Cattle Diseases etiology, Pregnancy, Animal physiology
- Abstract
Loss of pregnancy can occur at many different stages of gestation and for a variety of causes but clearly produces a negative impact for reproductive and economic performances of dairy herds. This review describes four pivotal periods for pregnancy loss during the first trimester of gestation and discusses possible causes for pregnancy failure during these periods. The first period occurs during the first week after breeding with lack of fertilization and death of the early embryo producing major losses in pregnancy, particularly under specific environmental and hormonal conditions. In general, 20%-50% of high-producing lactating dairy cows have already experienced pregnancy loss during the first week of gestation with methods to decrease pregnancy loss during this period targeting improved oocyte quality by alleviating heat stress, inflammatory diseases, and body condition loss, and by increasing progesterone concentrations during preovulatory follicle development. The second pivotal period, from Days 8 to 27, encompasses embryo elongation and the classical "maternal recognition of pregnancy" period with losses averaging ∼30% but with surprising variation between farms (25%-41%). Maintenance of the CL of pregnancy is produced by the embryonic signal interferon-tau and alteration in uterine secretory patterns of prostaglandins F2α, E1, and E2. Failures or delays in trophoblast elongation and/or embryonic development result in loss of pregnancy during the second pivotal period possibly due to suboptimal histotroph. The third pivotal period is during the second month of pregnancy, Days 28 to 60, with losses of ∼12% based on a summary of published results from more than 20,000 pregnancies in high-producing dairy cows. Delays or defects in development of the chorioallantoic placentomes or embryo result in CL regression or embryo death during this pivotal period. Finally, a fourth period during the third month of pregnancy has reduced pregnancy losses (∼2%), compared with the first three periods but can be elevated in some cows, particularly in those carrying twins in the same uterine horn. Thus, there are varied causes for pregnancy losses during each pivotal period that correspond to key physiological changes in the embryo, uterine environment, and ovary. Similarly, strategies to reduce these losses are likely to require a multifaceted approach using rational methods that target the critical physiology in each pivotal period., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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21. Identification of mycotoxins by UHPLC-QTOF MS in airborne fungi and fungi isolated from industrial paper and antique documents from the Archive of Bogotá.
- Author
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Castillo NI, Ibáñez M, Beltrán E, Rivera-Monroy J, Ochoa JC, Páez-Castillo M, Posada-Buitrago ML, Sulyok M, and Hernández F
- Subjects
- Air Pollution, Indoor, Archives, Chromatography, High Pressure Liquid, Colombia, DNA, Fungal analysis, DNA, Ribosomal analysis, Environmental Monitoring, Fungi genetics, Fungi isolation & purification, Paper, Sequence Analysis, DNA, Tandem Mass Spectrometry, Environmental Pollutants analysis, Mycotoxins analysis
- Abstract
Mold deterioration of historical documents in archives and libraries is a frequent and complex phenomenon that may have important economic and cultural consequences. In addition, exposure to toxic fungal metabolites might produce health problems. In this work, samples of broths of fungal species isolated from the documentary material and from indoor environmental samples of the Archive of Bogotá have been analyzed to investigate the presence of mycotoxins. High resolution mass spectrometry made possible to search for a large number of mycotoxins, even without reference standards available at the laboratory. For this purpose, a screening strategy based on ultra-high pressure liquid chromatography coupled to quadrupole-time of flight mass spectrometry (UHPLC-QTOF MS) under MS(E) mode was applied. A customized home-made database containing elemental composition for around 600 mycotoxins was compiled. The presence of the (de)protonated molecule measured at its accurate mass was evaluated in the samples. When a peak was detected, collision induced dissociation fragments and characteristic isotopic ions were also evaluated and used for tentative identification, based on structure compatibility and comparison with literature data (if existing). Up to 44 mycotoxins were tentatively identified by UHPLC-QTOF MS. 34 of these tentative compounds were confirmed by subsequent analysis using a targeted LC-MS/MS method, supporting the strong potential of QTOF MS for identification/elucidation purposes. The presence of mycotoxins in these samples might help to reinforce safety measures for researchers and staff who work on reception, restoration and conservation of archival material, not only at the Archive of Bogotá but worldwide., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2016
- Full Text
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22. A complex population structure of the cassava pathogen Xanthomonas axonopodis pv. manihotis in recent years in the Caribbean Region of Colombia.
- Author
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Trujillo CA, Ochoa JC, Mideros MF, Restrepo S, López C, and Bernal A
- Subjects
- Amplified Fragment Length Polymorphism Analysis, Colombia, DNA, Bacterial genetics, Evolution, Molecular, Genes, Bacterial, Genetic Markers, Haplotypes, Polymorphism, Genetic, Selection, Genetic, Virulence genetics, Xanthomonas axonopodis pathogenicity, Genetics, Population, Manihot microbiology, Plant Diseases microbiology, Xanthomonas axonopodis genetics
- Abstract
Cassava bacterial blight, caused by Xanthomonas axonopodis pv. manihotis (Xam), is the most important bacterial disease affecting this crop. A continuous surveillance of the pathogen population dynamics is required to develop an efficient disease management program. During the 1990s, Xam populations showed high levels of genetic variation and relevant migratory processes that were important determinants of the distribution of the pathogen diversity in Colombia. Aiming to characterize the current population structure of the pathogen and the evolutionary forces that shape these populations, sampling collections were carried out from September 2008 until November 2010 in the Colombian Caribbean Region. One hundred and sixty bacterial isolates were characterized using amplified fragment length polymorphism (AFLP) markers. Additionally, a subset of effector genes were sequenced in some isolates to determine their usefulness in Xam population studies and to provide additional information to that obtained with AFLPs. Virulence patterns of ten isolates were determined in nine cassava accessions. Our results show a complex architecture of population and confirm migratory process previously reported in the Caribbean Region. Chinú, one of the locations sampled, presented remarkable features in population dynamics such as longer genetic distances, higher diversity indices, and a genetically differentiated population when it was compared with other locations. Virulence tests showed that MCOL2215, one of the most cultivated cassava varieties in the Caribbean coast, was susceptible to the majority of Xam isolates tested. This study shows the current condition of populations of Xam in the Caribbean Region of Colombia, and it contributes to improve the existing bacterial blight control practices.
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- 2014
- Full Text
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23. Effect of shear stress and growth conditions on detachment and physical properties of biofilms.
- Author
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Paul E, Ochoa JC, Pechaud Y, Liu Y, and Liné A
- Subjects
- Hydrodynamics, Biofilms growth & development, Stress, Mechanical
- Abstract
Detachment is one of the major processes determining the physical structure and microbial functionalities of biofilms. To predict detachment, it is necessary to take the mechanical properties of the biofilm and the effect of both hydrodynamic and growth conditions into account. In this work, experiments were conducted with biofilms developed under various shear stresses and with various substrate natures. In addition, two cases were considered in order to differentiate between the effect of hydrodynamic factors and growth factors: the biofilms were directly grown under the targeted shear stress (τ) condition or they were precultivated under very low shear stress (0.01 Pa) and then exposed to high shear stress in the range of 0.1-13 Pa. An exponential and asymptotic decrease of the biofilm thickness and mass with increasing τ was observed in both cases. On contrary density, expressed as the biofilm dry mass on a known substratum divided by the average thickness increased with τ. Denitrifying biofilms always showed greater thickness and density than oxic biofilms. These results showed the presence of a compact basal layer that resisted shear stresses as high as 13 Pa whatever the culture conditions. Above this basal layer, the cohesion was lower and depended on the shear stress applied during biofilm development. The application of shear stress to the biofilms resulted in both detachment and compression, but detachment prevailed for the upper part of the biofilms and compression prevailed for the basal layers. A model of biofilm structure underlying the stratified character of this aggregate is given in terms of density and cohesion., (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
24. Recycled and virgin plastic carriers in hybrid reactors for wastewater treatment.
- Author
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Paul E, Wolff DB, Ochoa JC, and da Costa RH
- Subjects
- Biofilms, Carbon metabolism, Conservation of Natural Resources, Nitrogen metabolism, Bioreactors, Polyethylene, Waste Disposal, Fluid methods, Water Pollutants, Chemical metabolism
- Abstract
The reduction of organic and nitrogen pollution of wastewater was investigated in two hybrid reactors and compared with the reduction obtained by using a conventional activated sludge reactor (ASR) run as a control. Both HR-1 and HR-2 were activated sludge systems where a low-density carrier, P1 (polyethylene) for HR-1 and P2 (recycled plastics) for HR-2, was added. Firstly, the three reactors were operated at 10 days Suspended Solid Retention Time (SRT(SS)), leading to a complete nitrification. Secondly, the SRT(SS) for each reactor was lowered to 3 days. Nitrification was lost for the ASR but remained complete for HR's. Respirometric techniques were used to measure fixed or suspended biomass activities for heterotrophic and autotrophic biomass. More than 90% of the autotrophic activity was found on the supports whatever the SRT(SS) used. The results may underline the role of the carrier geometry or surface characteristics on the autotrophic/heterotrophic microorganism distribution.
- Published
- 2007
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25. Development and calibration of a nitrification PDE model based on experimental data issued from biofilter treating drinking water.
- Author
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Queinnec I, Ochoa JC, Wouwer AV, and Paul E
- Subjects
- Biofilms, Biomass, Calibration, Filtration, Models, Theoretical, Nitrobacter classification, Nitrobacter growth & development, Nitrogen analysis, Nitrosomonas europaea growth & development, Reproducibility of Results, Time Factors, Ammonia isolation & purification, Nitrobacter physiology, Nitrogen chemistry, Nitrosomonas europaea physiology, Water Purification methods, Water Supply
- Abstract
To remove ammonia for production of drinking water, nitrification can be performed in a bio-filter. At least 1 month is necessary to capture from the groundwater and then grow a sufficient amount of nitrifying bacteria to reach the desired removal efficiency. Improving start-up of bio-filters at low substrate concentration is therefore a major challenge. In this connection, it is important to develop appropriate models for designing, monitoring or analysing biofilm systems during start-up or following disinfection events. This study discusses the development and calibration of a nitrification PDE model which reflects the compromise between the complexity associated with the description of the full physical and biochemical mechanisms and the search for a simplified model with identifiable parameters. This model takes only the relevant phenomena (considering the full operating range) into account. The validity of the calibrated model has been evaluated through experiments under very different operational conditions, at the laboratory and under real industrial conditions, involving the full upstream chain of water treatment (iron oxidation and sand filter)., (Copyright 2006 Wiley Periodicals, Inc.)
- Published
- 2006
- Full Text
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26. Active heterotrophic and autotrophic biomass distribution between fixed and suspended systems in a hybrid biological reactor.
- Author
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Ochoa JC, Colprin J, Palacios B, Paul E, and Chatellier P
- Subjects
- Bacteria, Biomass, Kinetics, Oxygen metabolism, Population Dynamics, Waste Disposal, Fluid, Bioreactors, Sewage microbiology
- Abstract
This paper presents the results obtained when modifying sludge retention time (SRT 8, 5, and 3.7 days during phases A, B and C respectively) for a hybrid biological reactor (HR) compared with a classical activated sludge process. The study was conducted by following active biomass evolution and distribution for two lab-scale pilots plants operating with the same conditions, one acting as HR and the other as the control reactor (CR) without support material. At the end of phase C, support material was split into two fractions between both reactors to study the effect of support to reactor volume ratio (Fr). Active biomasses in suspended and fixed systems were calculated using respirometric techniques. Evolutions of active autotrophic and heterotrophic biomasses for both reactors are presented during all the operational periods and it is observed that in the HR biomass concentrations are up to double that in the CR, mainly due to the presence of support material. When studying biomass distribution in HR, autotrophic biomass is mainly located over the support material (from 95% to 99% during periods A and C respectively) while only about 60% of heterotrophic biomass is located over the support.
- Published
- 2002
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