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Cerebral malaria is a complex neurological syndrome caused by an infection with Plasmodium falciparum parasites and is exclusively attributed to a series of host–parasite interactions at the pathological blood-stage of infection. In contrast, the preceding intra-hepatic phase of replication is generally considered clinically silent and thereby excluded from playing any role in the development of neurological symptoms. In this study, however, we present an antigen PbmaLS_05 that is presented to the host immune system by both pre-erythrocytic and intra-erythrocytic stages and contributes to the development of cerebral malaria in mice. Although deletion of the endogenous PbmaLS_05 prevented the development of experimental cerebral malaria (ECM) in susceptible mice after both sporozoite and infected red blood cell (iRBC) infections, we observed significant differences in contribution of the host immune response between both modes of inoculation. Moreover, PbmaLS_05-specific CD8+ T cells contributed to the development of ECM after sporozoite but not iRBC-infection, suggesting that pre-erythrocytic antigens like PbmaLS_05 can also contribute to the development of cerebral symptoms. Our data thus highlight the importance of the natural route of infection in the study of ECM, with potential implications for vaccine and therapeutic strategies against malaria.

This dissertation thesis "Enforcement of maintenance collection in regard of efficiency of the functioning of law in a society", deals with the term of the law impact efficiency in the society and its practical effect on the institute of prosecution of a maintenance collection, focused on maintenance collection for minor children. The dissertation aims to analyze the process of the enforcement of the maintenance and to examine its efficiency. This dissertation is elaborated mainly on the basis of the Czech academic literature, the newly amended laws, the reliable printed and online sources and the germane court's interpretations. The dissertation consists of three chapters, which are further divided into subchapters. The first chapter provides description of the institute of the maintenance from the Czech operative law point of view. The second chapter deals with the problem of the law impact efficiency itself in the society. It defines the theme itself and describes its main barriers in regard of the institute of the maintenance. The third chapter examines the problem of the efficiency of the enforcement of a maintenance collection. The conclusion of the dissertation summarizes ascertained knowledge and examines the efficiency of the enforcement of a maintenance collection.