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Unilateral cryptorchidism was induced in adult rats for 5, 10 and 15 days and its influence on testicular morphology was investigated. In seminiferous tubules from abdominal testes the degenerative changes of germ cells was manifested in spermatocytes, spermatids and spermatozoa in Day 5 after the induction of cryptorchidism. These germ cells were almost absent by 15 days after the induction of cryptorchidism. On the other hand, spermatogonia and Sertoli cells were maintained in the failure of some morphological damages during experimental cryptorchidism. It is suggested that spermatocytes and young spermatids like spermatocytes, spermatids and spermatozoa were the most sensitive germ cells to body heat, whereas spermatogonia and Sertoli cells showed strong resistance to it. It was thought that multinucleated giant cells appeared at early time following artificial cryptorchidism might be responsible for the elimination of degenerated germ cells through phagacytosis by the cells., ラット精巣に対する温度処理が精子形成に如何なる影響を及ぼすかを調べるため，精巣の場所である陰嚢より1～8℃高い温度下にある腹腔へ精巣を人為的に押し込み，結紮によって精巣の脱出を防いで腹腔内停留精巣を作成した。停留処置後5日，10日および15日目に精巣を摘出し，組織標本を作製しそ精巣精細管における組織像を観察した。精巣の停留処置を行わなかった片側の陰嚢内精巣は対照として用いた。その結果，精巣停留処置後5日目の精細管で，すでに各種精細胞に退行的変化が観察され，特にその変化は精母細胞，精娘細胞，精子細胞および精子に出現した。また，精細胞の変化に伴って貪食作用を有する巨大多核細胞が出現した。精細胞における退行変化は精巣の腹腔内停留期間が延長するにつれ顕著になり，処置後15日目の精細管では精祖細胞以外の精細胞は完全に消失した。同様な退行的変化は精子の通路である精巣上体管においても観察された。処置後5日目で精巣上体内精子集落に巨大多核細胞が出現し，処置後10日では精子集落が赤色球形細胞に変化し，15日目では精巣上体管は精細胞を欠き，その内腔は空白を呈した。, 紀要論文

Unilateral cryptorchidism was induced in adult rats for 5, 10 and 15 days and its influence on testicular morphology was investigated. In seminiferous tubules from abdominal testes the degenerative changes of germ cells was manifested in spermatocytes, spermatids and spermatozoa in Day 5 after the induction of cryptorchidism. These germ cells were almost absent by 15 days after the induction of cryptorchidism. On the other hand, spermatogonia and Sertoli cells were maintained in the failure of some morphological damages during experimental cryptorchidism. It is suggested that spermatocytes and young spermatids like spermatocytes, spermatids and spermatozoa were the most sensitive germ cells to body heat, whereas spermatogonia and Sertoli cells showed strong resistance to it. It was thought that multinucleated giant cells appeared at early time following artificial cryptorchidism might be responsible for the elimination of degenerated germ cells through phagacytosis by the cells., ラット精巣に対する温度処理が精子形成に如何なる影響を及ぼすかを調べるため，精巣の場所である陰嚢より1～8℃高い温度下にある腹腔へ精巣を人為的に押し込み，結紮によって精巣の脱出を防いで腹腔内停留精巣を作成した。停留処置後5日，10日および15日目に精巣を摘出し，組織標本を作製しそ精巣精細管における組織像を観察した。精巣の停留処置を行わなかった片側の陰嚢内精巣は対照として用いた。その結果，精巣停留処置後5日目の精細管で，すでに各種精細胞に退行的変化が観察され，特にその変化は精母細胞，精娘細胞，精子細胞および精子に出現した。また，精細胞の変化に伴って貪食作用を有する巨大多核細胞が出現した。精細胞における退行変化は精巣の腹腔内停留期間が延長するにつれ顕著になり，処置後15日目の精細管では精祖細胞以外の精細胞は完全に消失した。同様な退行的変化は精子の通路である精巣上体管においても観察された。処置後5日目で精巣上体内精子集落に巨大多核細胞が出現し，処置後10日では精子集落が赤色球形細胞に変化し，15日目では精巣上体管は精細胞を欠き，その内腔は空白を呈した。

The Mirasol system has been demonstrated to effectively inactivate white blood cells (WBCs) and reduce pathogens in whole blood in vitro. The purpose of this study was to compare the safety and efficacy of Mirasol-treated fresh whole blood (FWB) to untreated FWB in an in vivo model of surgical bleeding.A total of 18 anesthetized pigs (40 kg) underwent a 35% total blood volume bleed, cooling to 33°C, and a standardized liver injury. Animals were then randomly assigned to resuscitation with either Mirasol-treated or untreated FWB, and intraoperative blood loss was measured. After abdominal closure, the animals were observed for 14 days, after which the animals were euthanized and tissues were obtained for histopathologic examination. Mortality, tissue near-infrared spectroscopy, red blood cell (RBC) variables, platelets (PLTs), WBCs, and coagulation indices were analyzed.Total intraoperative blood loss was similar in test and control arms (8.3 ± 3.2 mL/kg vs. 7.7 ± 3.9 mL/kg, p = 0.720). All animals survived to Day 14. Trended values over time did not show significant differences-tissue oxygenation (p = 0.605), hemoglobin (p = 0.461), PLTs (p = 0.807), WBCs (p = 0.435), prothrombin time (p = 0.655), activated partial thromboplastin time (p = 0.416), thromboelastography (TEG)-reaction time (p = 0.265), or TEG-clot formation time (p = 0.081). Histopathology did not show significant differences between arms.Mirasol-treated FWB did not impact survival, blood loss, tissue oxygen delivery, RBC indices, or coagulation variables in a standardized liver injury model. These data suggest that Mirasol-treated FWB is both safe and efficacious in vivo.