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319 results on '"Obara, Keisuke"'

7. Eicosapentaenoic acid (EPA)-induced inhibitory effects on porcine coronary and cerebral arteries involve inhibition of prostanoid TP receptors

Author

Yoshioka, Kento, Obara, Keisuke, Oikawa, Shunya, Uemura, Kohei, Yamaguchi, Akina, Fujisawa, Kazuki, Hanazawa, Hitomi, Fujiwara, Miki, Endoh, Taison, Suzuki, Taichi, De Dios Regadera, Montserrat, Ito, Daichi, Saitoh, Noboru, Nakagome, Yutaka, Yamashita, Toma, Kiguchi, Mayu, Saito, Yuka, Nakao, Yuri, Miyaji, Hinako, Ou, Guanghan, Xu, Keyue, and Tanaka, Yoshio

Published
2022
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16. Platelet-activating factor contracts guinea pig esophageal muscularis mucosae by stimulating extracellular Ca2+influx through diltiazem-insensitive Ca2+channels

Author

Obara, Keisuke, Ichimura, Aina, Arai, Taichi, Fujiwara, Mako, Otake, Miho, Yamada, Nana, Yoshioka, Kento, Kusakabe, Taichi, Takahashi, Keisuke, Kato, Keisuke, and Tanaka, Yoshio

Abstract

Platelet-activating factor (PAF) is expected to increase esophageal motility. However, to the best of our knowledge, this has not been examined. Thus, we investigated the contractile effects of PAF on guinea pig (GP) esophageal muscularis mucosae (EMM) and the extracellular Ca2+influx pathways responsible. PAF (10−9–10−6 M) contracted EMM in a concentration-dependent manner. PAF (10−6 M)-induced contractions were almost completely suppressed by apafant (a PAF receptor antagonist, 3 × 10−5 M). In EMM strips, PAF receptor and PAF-synthesizing/degrading enzyme mRNAs were detected. PAF (10−6 M)-induced contractions were abolished by extracellular Ca2+removal but were not affected by diltiazem [a voltage-dependent Ca2+channel (VDCC) inhibitor, 10−5 M]. PAF (10−6 M)-induced contractions in the presence of diltiazem were significantly suppressed by LOE-908 [a receptor-operated Ca2+channel (ROCC) inhibitor, 3 × 10−5 M], SKF-96365 [an ROCC and store-operated Ca2+channel (SOCC) inhibitor, 3 × 10−5 M], and LOE-908 plus SKF-96365. Among the tested ROCC/SOCC-related mRNAs, Trpc3, Trpc6, and Trpv4/Orai1, Orai3, and Stim2were abundantly expressed in EMM strips. These results indicate that PAF potently induces GP EMM contractions that are dependent on extracellular Ca2+influx through ROCCs/SOCCs, and VDCCs are unlikely to be involved.

Published
2024
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21. Regulator of Awn Elongation 3 , an E3 ubiquitin ligase, is responsible for loss of awns during African rice domestication

Author

Bessho-Uehara, Kanako, primary, Masuda, Kengo, additional, Wang, Diane R., additional, Angeles-Shim, Rosalyn B., additional, Obara, Keisuke, additional, Nagai, Keisuke, additional, Murase, Riri, additional, Aoki, Shin-ichiro, additional, Furuta, Tomoyuki, additional, Miura, Kotaro, additional, Wu, Jianzhong, additional, Yamagata, Yoshiyuki, additional, Yasui, Hideshi, additional, Kantar, Michael B., additional, Yoshimura, Atsushi, additional, Kamura, Takumi, additional, McCouch, Susan R., additional, and Ashikari, Motoyuki, additional

Published
2023
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24. Development of AlissAID system targeting GFP or mCherry fusion protein.

Author

Ogawa, Yoshitaka, Nishimura, Kohei, Obara, Keisuke, and Kamura, Takumi

Subjects
CHIMERIC proteins, GREEN fluorescent protein, FLUORESCENT proteins, SYSTEMS development, IMMUNE recognition, IMMUNOGLOBULINS
Abstract

Conditional control of target proteins using the auxin-inducible degron (AID) system provides a powerful tool for investigating protein function in eukaryotes. Here, we established an Affinity-linker based super-sensitive auxin-inducible degron (AlissAID) system in budding yeast by using a single domain antibody (a nanobody). In this system, target proteins fused with GFP or mCherry were degraded depending on a synthetic auxin, 5-Adamantyl-IAA (5-Ad-IAA). In AlissAID system, nanomolar concentration of 5-Ad-IAA induces target degradation, thus minimizing the side effects from chemical compounds. In addition, in AlissAID system, we observed few basal degradations which was observed in other AID systems including ssAID system. Furthermore, AlissAID based conditional knockdown cell lines are easily generated by using budding yeast GFP Clone Collection. Target protein, which has antigen recognition sites exposed in cytosol or nucleus, can be degraded by the AlissAID system. From these advantages, the AlissAID system would be an ideal protein-knockdown system in budding yeast cells. Author summary: Budding yeast has been widely studied as a genetic model organism for eukaryotic species. Targeted degradation systems, in which conditional-control of target protein degradation, are used to examine protein function. The auxin-inducible degron (AID) system is one such system that rapidly disrupts target proteins in an auxin (IAA)-dependent manner. In this system, high concentrations of auxin and AID-tagging to the target proteins are necessary for degradation. Here, we developed the Affinity-linker based super-sensitive auxin-inducible degron (AlissAID) system, which enables to degrade target proteins with nM concentration of a synthetic auxin, 5-Adamantyl-IAA (5-Ad-IAA). In this system, we can use popular fluorescent proteins GFP or mCherry as degradation tags and avoid AID-tagging to the target endogenous gene. As GFP Clone Collection is commercially available in budding yeast, it is easy to generate conditional mutants in AlissAID system. AlissAID system would be a powerful tool of genetic analysis in eukaryotic species including budding yeast. [ABSTRACT FROM AUTHOR]

Published
2023
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27. Docosahexaenoic acid and eicosapentaenoic acid strongly inhibit prostanoid TP receptor‐dependent contractions of guinea pig gastric fundus smooth muscle

Author

Xu, Keyue, primary, Shimizu, Miyuki, additional, Murai, Chika, additional, Fujisawa, Miki, additional, Ito, Daichi, additional, Saitoh, Noboru, additional, Nakagome, Yutaka, additional, Yamashita, Mio, additional, Murata, Azusa, additional, Oikawa, Shunya, additional, Ou, Guanghan, additional, Yoshioka, Kento, additional, Obara, Keisuke, additional, and Tanaka, Yoshio, additional

Published
2022
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30. Platelet-activating factor (PAF) enhances guinea pig detrusor smooth muscle contractile activities by stimulating voltage-dependent Ca2+channels and store-operated Ca2+channels

Author

Obara, Keisuke, Kaneko, Mizuki, Yamashita, Mio, Liu, Ge, Yoshioka, Kento, and Tanaka, Yoshio

Abstract

We investigated the extracellular Ca2+influx pathways involved in platelet-activating factor (PAF)-enhanced guinea pig detrusor smooth muscle (DSM) contractile activities. One micromolar PAF-enhanced DSM contractile activities were completely inhibited by extracellular Ca2+removal and strongly suppressed by voltage-dependent Ca2+channel (VDCC) inhibitors. PAF-enhanced DSM contractile activities remaining in the presence of verapamil (10 μM) were not inhibited by LOE-908 (30 μM, an inhibitor of receptor-operated Ca2+channels (ROCCs)), but were almost completely inhibited by SKF-96365 (30 μM, an inhibitor of store-operated Ca2+channels (SOCCs) and ROCCs). These results suggest that VDCCs and SOCCs are responsible for PAF-enhanced DSM contractile activities.

Published
2023
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32. Docosahexaenoic acid (DHA) selectively inhibits prostanoid TP receptor-mediated contractions of guinea pig tracheal smooth muscle

Author

Obara, Keisuke, primary, Inaba, Rikako, additional, Kawakita, Mirai, additional, De Dios Regadera, Montserrat, additional, Uetake, Tomomi, additional, Murata, Azusa, additional, Nishioka, Nanako, additional, Kuroki, Kota, additional, Oikawa, Shunya, additional, Yoshioka, Kento, additional, and Tanaka, Yoshio, additional

Published
2022
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33. Inhibitory effects of eicosapentaenoic acid (EPA) on contractions in pig basilar and coronary arteries

Author

Yoshioka, Kento, primary, Obara, Keisuke, additional, Oikawa, Shunya, additional, Uemura, Kohei, additional, Yamaguchi, Akina, additional, Fujisawa, Kazuki, additional, Hanazawa, Hitomi, additional, Fujiwara, Miki, additional, Endoh, Taison, additional, Suzuki, Taichi, additional, De Dios Regadera, Montserrat, additional, Ito, Daichi, additional, Saitoh, Noboru, additional, Nakagome, Yutaka, additional, Yamashita, Toma, additional, Kiguchi, Mayu, additional, Saito, Yuka, additional, Nakao, Yuri, additional, Miyaji, Hinako, additional, Ou, Guanghan, additional, Xu, Keyue, additional, and Tanaka, Yoshio, additional

Published
2022
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35. Docosahexaenoic acid inhibits U46619- and prostaglandin F2α-induced pig coronary and basilar artery contractions by inhibiting prostanoid TP receptors

Author

Yoshioka, Kento, primary, Obara, Keisuke, additional, Oikawa, Shunya, additional, Uemura, Kohei, additional, Yamaguchi, Akina, additional, Fujisawa, Kazuki, additional, Hanazawa, Hitomi, additional, Fujiwara, Miki, additional, Endoh, Taison, additional, Suzuki, Taichi, additional, De Dios Regadera, Montserrat, additional, Ito, Daichi, additional, Ou, Guanghan, additional, Xu, Keyue, additional, and Tanaka, Yoshio, additional

Published
2021
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37. Inhibitory Effects of Antipsychotics on the Contractile Response to Acetylcholine in Rat Urinary Bladder Smooth Muscles

Author

Obara, Keisuke, primary, Matsuoka, Yuka, additional, Iwata, Naoya, additional, Abe, Yukako, additional, Ikegami, Yohei, additional, Shioda, Nanako, additional, Hattori, Yume, additional, Hamamatsu, Shoko, additional, Yoshioka, Kento, additional, Yamaki, Fumiko, additional, Matsuo, Kazuhiro, additional, Yoshio, Takashi, additional, and Tanaka, Yoshio, additional

Published
2021
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39. PI3K signaling of autophagy is required for starvation tolerance and virulence of Cryptococcus neoformans

Author

Hu, Guowu, Hacham, Moshe, Waterman, Scott R., Panepinto, John, Shin, Soowan, Liu, Xiaoguang, Gibbons, Jack, Valyi-Nagy, Tibor, Obara, Keisuke, Jaffe, H. Ari, Ohsumi, Yoshinori, and Williamson, Peter R.

Subjects
Cryptococcus -- Health aspects, Cryptococcus -- Genetic aspects, Cryptococcus -- Research, Gene expression -- Research, Infection -- Causes of, Infection -- Care and treatment, Infection -- Genetic aspects, Infection -- Research
Abstract

Autophagy is a process by which cells recycle cytoplasm and defective organelles during stress situations such as nutrient starvation. It can also be used by host cells as an immune defense mechanism to eliminate infectious pathogens. Here we describe the use of autophagy as a survival mechanism and virulence-associated trait by the human fungal pathogen Cryptococcus neoformans. We report that a mutant form of C. neoformans lacking the Vps34 PI3K (vps34[DELTA]), which is known to be involved in autophagy in ascomycete yeast, was defective in the formation of autophagy-related 8-labeled (Atg8-labeled) vesicles and showed a dramatic attenuation in virulence in mouse models of infection. In addition, autophagic vesicles were observed in WT but not vps34[DELTA] cells after phagocytosis by a murine macrophage cell line, and Atg8 expression was exhibited in WT C. neoformans during human infection of brain. To dissect the contribution of defective autophagy in vps34[DELTA] C. neoformans during pathogenesis, a strain of C. neoformans in which Atg8 expression was knocked down by RNA interference was constructed and these fungi also demonstrated markedly attenuated virulence in a mouse model of infection. These results demonstrated PI3K signaling and autophagy as a virulence-associated trait and survival mechanism during infection with a fungal pathogen. Moreover, the data show that molecular dissection of such pathogen stress-response pathways may identify new approaches for chemotherapeutic interventions., Introduction Cryptococcus neoformans (Cn) is a major fungal pathogen primarily infecting immunocompromised hosts, especially patients with impaired cellular immunity related to HIV infection (1). It is generally believed that inhalation [...]

Published
2008

40. Assessment of the effects of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on the contractile responses of guinea pig lower gastrointestinal (LGI) tracts

Author

Obara, Keisuke, primary, Xu, Keyue, additional, Ou, Guanghan, additional, Inaba, Rikako, additional, Kawakita, Mirai, additional, Kawaguchi, Ayana, additional, Kudo, Rena, additional, Kurata, Akari, additional, Yoshioka, Kento, additional, Yamaki, Fumiko, additional, and Tanaka, Yoshio, additional

Published
2021
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