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409 results on '"Oba, Eunice"'

1. Heat stress induced by testicular insulation for 24 or 48 h rapidly impairs epididymal sperm quality and reduces spermatogenesis in rams

Author

Teixeira, Marina Belucci, Ferreira, João Carlos Pinheiro, Codognoto, Viviane Maria, Rossi, Eduardo Santos, Pupulim, Antonio Guilherme Roncado, de Carvalho, Jaqueline Cândido, Rattes, Paula Zanin, Oba, Eunice, Navolar, Felipe Martins Negreiro, Di Santis, Giovana Wingeter, Kastelic, John Patrick, Van Soom, Ann, and Rizzoto, Guilherme

Published
2025
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13. Use of coenzyme Q-10 to improve the pregnancy rate in sheep

Author

Tironi, Stella Maris Teobaldo, primary, Sitó-Silva, Luan, additional, de Camillo, Beatriz Lippe, additional, Denadai, Renan, additional, Silva, Adrielly Lais Alves da, additional, de Paula Freitas-Dell’Aqua, Camila, additional, Junior, José Antonio Dell’Aqua, additional, de Oliveira, Rogério Antonio, additional, Souza, Maria Inês Lenz, additional, and Oba, Eunice, additional

Published
2024
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14. The use of sodium caseinate in the freezing of sheep semen

Author

Salgado, Letícia C., primary, Codognoto, Viviane M., additional, Garcia, Rodrigo, additional, Canuto, Lucas E. F., additional, Denadai, Renan, additional, Freitas‐Dell'Aqua, Camila P., additional, Almeida Filho, Rogério A., additional, Sitó‐Silva, Luan, additional, Crespilho, André M., additional, and Oba, Eunice, additional

Published
2024
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18. Effects of antioxidant Bis‐carboxyethyl germanium sesquioxide added to bovine semen cryopreservation medium on in vitro assessed morphofunctional sperm parameters.

Author

Cruz, Tairini E., Sitó‐Silva, Luan, Filho, Rogério A. Almeida, Martins, Alicio, Marqui, Fernanda N., Souza, Diego G., Berton, Tatiana I. U., Freita‐Dell'Aqua, Camila P., and Oba, Eunice

Subjects
REACTIVE oxygen species, ARACHIDONIC acid, OXIDATIVE stress, SPERMATOZOA, GERMANIUM
Abstract

This study investigated the impact of various Ge132 (Bis‐carboxyethyl germanium sesquioxide) concentrations on frozen bovine semen. Ejaculates from three bulls were pooled and divided into six groups, each one with different Ge132 concentrations (0, 500, and 1000 μg/mL) and each group was incubated in different conditions (33°C for 30 min (D: D0, D500, and D1000), and the other was immediately cooled to 4°C (R: R0‐control; R500 and R1000)). Thawed semen was evaluated for sperm characteristics by CASA and flow cytometer. Results showed better motility in the immediate cooling group without Ge132 compared with high Ge132 concentrations. Values for total motility dropped after 5 and 60 min in groups with high Ge132 levels and some control groups. Linearity increased with 1000 μg/mL Ge132, while straightness differed between moments in multiple groups. Membrane integrity was higher in a control group and certain Ge132 groups. Lower O2− generation occurred without Ge132. After oxidative stress induction, lipid peroxidation intensity increased with arachidonic acid, but D1000 had lower peroxidation than R0. Overall, Ge132 appears to have provided protection against PLM when subjected to oxidative stress, since even at high concentrations it maintained sperm metabolism. [ABSTRACT FROM AUTHOR]

Published
2024
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19. Effect of pre‐treatment with deslorelin on the ovarian response of ewes superovulated with FSH.

Author

Garcia, Rodrigo, Filho, Rogério A. Almeida, Sitó‐Silva, Luan, Denadai, Renan, Codognoto, Viviane, Salgado, Letícia, Brochine, Suzane, and Oba, Eunice

Subjects
CORPUS luteum, GONADOTROPIN releasing hormone, EMBRYO transfer, EWES, OVULATION, OVARIAN follicle
Abstract

This study evaluated the use of the GnRH agonist hormone, deslorelin, to control the follicular population before initiating multiple ovulation and embryo transfer (MOET) treatment. Twenty‐four cross‐bred Santa Inês ewes, aged between 2 and 4 years, were randomly assigned to either a control group (n = 11) or a treated group (n = 13). All ewes received an intravaginal device containing 60 mg of medroxyprogesterone acetate on day 0, and a new device on day 7, which remained in place until day 14. Additionally, the ewes were administered 125 μg of cloprostenol on day 7. The superovulatory treatment involved administering 200 mg of pFSH, divided into eight decreasing doses at 12‐h intervals starting on day 12. On day 14, 300 IU of eCG was administered. In the deslorelin group, three doses of 100 μg of deslorelin were administered starting on day 3 after the insertion of the vaginal device, with subsequent doses given at 72‐h and 144‐h intervals. Natural mating was performed 36 h after the removal of the progesterone implant using males with proven fertility. Embryo collection took place on the 6th day after mating, and the recovered structures were quantified and evaluated for quality and developmental stage. Transrectal ultrasonography was conducted on days 12, 16 and 21 to evaluate the ovaries, specifically to assess the ovarian follicular population and the presence of the corpus luteum. Ewes in the control group had higher embryo recovery rates (p <.01) compared to the treated group (5.2 ± 0.8 vs. 1.1 ± 0.8), with differences observed primarily in the number of morulae. The number of corpus luteum observed during the laparotomy on day 21 was significantly higher (p <.01) in the control group (10.44 vs. 4.5 corpus luteum per ewe). Yet, the treated group had a significantly higher number of follicles (p <.05) on the first day of pFSH application (5.5 vs. 3.0 follicles per ewe). In conclusion, although the inclusion of deslorelin in the superovulation protocol resulted in increased synchronization of oestrus and follicle number, it did not lead to an increase in the number of corpus luteum or harvested embryos. [ABSTRACT FROM AUTHOR]

Published
2024
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22. MANGANESE AND ITS ANTAGONISTIC AND SYNERGISTIC INTERACTIONS BETWEEN THE MINERAL ELEMENTS ON THE REPRODUCTION AND METABOLISM OF BULLS/O MANGANES E SUAS INTERACOES ANTAGONICAS E SINERGICAS ENTRE OS ELEMENTOS MINERAIS SOBRE A REPRODUCAO E O METABOLISMO DE TOUROS/EL MANGANESO Y SUS INTERACCIONES ANTAGONICAS Y SINERGICAS ENTRE LOS ELEMENTOS MINERALES SOBRE LA REPRODUCCION Y EL METABOLISMO DE TOROS

Author

Brandao Bueno, Ericka Stephanny, Messias de Souza, Adriano, Guinossi Barbosa Deak, Fernanda Luiza, de Andrade Bernal Fagiani, Marcela, Oba, Eunice, Biagio Chiacchio, Simone, and Souza Lima de Souza Reis, Luis

Published
2019

29. Evaluation of pharmacological alternatives to reduce the pain and discomfort produced by electroejaculation in rams.

Author

da Silva, Mariana Karla Francolino, de Almeida Gélio, Leonardo, Oba, Eunice, de Freitas, Douglas Anderson, Green, Renata Elisa, Nichi, Marcílio, and Crespilho, André Maciel

Subjects
NEURAL stimulation, ANIMAL sound production, ELECTRIC stimulation, SEMEN, CREATINE kinase, RAMS, FENTANYL
Abstract

Electroejaculation (EE) represents the main technique for semen collection from domestic and wild animals independently of libido. However, the technique is associated with intense involuntary muscle contractions, vocalization, ataxia and lying down, caused by the electric stimulation of the nerves in the caudal epigastric region. These clinical manifestations represent important indicators of discomfort. In this context, the objective of this study was to evaluate two protocols of local anaesthetic blockade and two anatomical access for pharmacological desensitization of the caudal epigastric innervation as alternatives to promote comfort and reduce stress associated with EE in rams. For the study, four clinically healthy Dorper rams were selected. All animals were subjected to a design consisting of five semen collection treatments (n = 3 collections per treatment): T1—control, conventional EE without local anaesthetic blockade; T2, EE with ventral blockade (VB) of epigastric innervation using lidocaine hydrochloride 2%; T3, EE with VB of epigastric innervation using a combination of lidocaine hydrochloride 2% and fentanyl citrate; T4, EE with blockade of epigastric innervation through the perineal access using lidocaine hydrochloride 2%; T5, EE with blockade of epigastric innervation through the perineal access using a combination of lidocaine hydrochloride and fentanyl citrate. Seminal samples resulting from EE were subjectively evaluated for sperm motility and concentration, vigour and volume. Additionally, blood serum samples were collected for quantification of cortisol and creatine kinase (CK) enzyme. Assessments of stress and discomfort were conducted by measuring blood pressure, heart rate (HR) and respiratory rate (RR), as well as observing involuntary muscle contractions, ataxia and animal vocalization. No variations in blood pressure, sperm motility, vigour, CK, and cortisol were observed among the treatments. Individual variations were observed for the occurrence of vocalization (p =.0066), but there were no differences between the groups. Anaesthetic blockades conducted using the combination of lidocaine and fentanyl resulted in a lower incidence of ataxia during EE (p <.0001). It is concluded that the combination of fentanyl citrate and lidocaine hydrochloride results in less discomfort for animals undergoing EE, regardless of the anatomical access used for local anaesthetic blockades. [ABSTRACT FROM AUTHOR]

Published
2024
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30. Production and hematological parameters of heifers ½ Angus ½ Nellore raised in integrated livestock-forestry system

Author

Gonçalves, Juliene Cruz, primary, Andrighetto, Cristiana, additional, Mateus, Gustavo Pavan, additional, Simiele, Carolina Barone, additional, Sekiya, Bianca Midori Souza, additional, Duarte, Julia Paschoareli Ferro, additional, Oba, Eunice, additional, Dias, Beatriz Poletto Neris, additional, Silva, Ben Yur Aparecido da, additional, Martinez, Edgar Ramos, additional, Oliveira, Juliana Moura, additional, and Gonela, Renan Fabrício, additional

Published
2023
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32. Temporal analysis of prostaglandin F2α receptor, caspase 3, and cyclooxygenase 2 messenger RNA expression and prostaglandin F2α receptor and cyclooxygenase 2 protein expression in endometrial tissue from multiparous Nelore (Bos taurus indicus) cows treated with cloprostenol sodium during puerperium

Author

de Moraes, Carolina N., Maia, Leandro, de Lima, Paula F., Dias, Marianne C., Raposo-Ferreira, Talita M.M., Sudano, Mateus J., Junior, José B., and Oba, Eunice

Published
2015
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33. Two Types of Management for the Noninvasive Treatment of Pectus Excavatum in Neonatal Puppies—Case Reports

Author

Pereira, Keylla Helena Nobre Pacífico, primary, Fuchs, Kárita da Mata, additional, Terçariol, Lara Ataídes Arantes, additional, Silva, Renata Cesar, additional, Camargo, Gabriel de Azevedo, additional, Mendonça, Júlia Cosenza, additional, Paulino, Netelin Tainara, additional, Zone, Marcelo Alejandro, additional, Oba, Eunice, additional, and Lourenço, Maria Lucia Gomes, additional

Published
2023
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40. Effect of quercetin or butylated hydroxytoluene on cooled or frozen-thawed ram sperm quality

Author

Canuto, Lucas Emanuel Ferreira [UNESP], Segabinazzi, Lorenzo Garrido Teixeira Martini [UNESP], De Araújo, Endrigo Adonis Braga [UNESP], Silva, Luis Fernando Mercês Chaves [UNESP], De Oliveira, Sidnei Nunes [UNESP], Dalanezi, Felipe Morales [UNESP], Dell'Aqua, José Antonio [UNESP], Papa, Frederico Ozanam [UNESP], Oba, Eunice [UNESP], and Universidade Estadual Paulista (UNESP)

Subjects
Ovinos, Cryopreservation, Sheep, urogenital system, Lipid peroxidation, Small ruminants, Criopreservação dano oxidativo, Oxidative damage, Antioxidante, Pequenos ruminantes, Antioxidant, Peroxidação lipídica, General Agricultural and Biological Sciences
Abstract

Cooling and freezing processes cause physical and chemical damage to sperm by cold shock and oxidative stress. This study aimed to evaluate the effect of two antioxidants on sperm parameters of cooled and frozen-thawed ram semen diluted in an egg yolk-based extender. Semen was collected from 30 rams and processed in two consecutive experiments to test the inclusion of different concentrations of quercetin and butylated hydroxytoluene (BHT) in an egg yolk-based semen extender. Dimethyl sulfoxide (DMSO) was added as a solvent to the semen extender in a ratio of 1 mL DMSO for 90 mg of quercetin and 1 mL DMSO for 880 mg of BHT. After collection, semen was diluted at 200 × 106 motile sperm/mL (control) and split into different groups in each experiment. In experiment 1, semen was diluted with the extender containing quercetin (Q5, 5 µg/mL; Q10, 10 ug/mL; Q15, 15 ug/mL) or DMSO alone (DMSO1, 0.055 uL DMSO per mL; DMSO2, 0.165 uL DMSO per mL). In experiment 2, semen was diluted with the extender with BHT (BHT1, 0.5 ug/mL; BHT2, 1 ug/mL; BHT3, 1.5 ug/mL) or DMSO alone (DMSO3, 0.375 uL DMSO per mL; DMSO4, 1.125 uL DMSO per mL). After dilution, the semen was divided into two aliquots. Treated ram sperm samples were also subjected to different storage methods. The first set of samples was cooled at 5 °C for 24 h, whereas the second set of samples was frozen-thawed. Sperm motility parameters and plasma membrane integrity (PMI) were evaluated immediately after dilution (0h) and 24 h after cooling and in the frozen-thawed samples via computer-assisted sperm analysis and epifluorescence microscopy, respectively. The inclusion of quercetin or BHT did not affect sperm motility parameters or PMI of fresh, cooled, or frozen-thawed sperm in this study (P < 0.05). However, further studies are needed to test the effects of these antioxidants on the fertility of cryopreserved ram semen. O resfriamento e o congelamento causam danos físicos e químicos aos espermatozoides por choque térmico e estresse oxidativo. Portanto, este estudo teve como objetivo avaliar o efeito da inclusão de dois antioxidantes em um diluente à base de gema de ovo sobre os parâmetros espermáticos do sêmen ovino resfriado e congelado. Trinta carneiros tiveram o sêmen coletado e processado em dois experimentos consecutivos para testar a inclusão de diferentes concentrações de quercetina e hidroxitolueno butilado (BHT) em diluente de sêmen à base de gema de ovo. O DMSO foi adicionado como solvente ao diluente de sêmen em uma proporção de 1 mL de DMSO parra 90 mg de quercetina e 1 Ml de DMSO para 880 mg de BHT. Após a coleta, o sêmen foi diluído a 200 × 106 espermatozoides móveis/mL (Controle) e dividido em diferentes grupos em cada experimento. Experimento 1, Quercetina (Q5, 5 ug / mL; Q10, 10 µg / mL; Q15, 15 ug / mL) ou DMSO (DMSO1, 0,055 uL de DMSO por ml; DMSO2, 0,165 uL de DMSO / mL) foram adicionados ao extensor. Experimento 2, BHT (BHT1, 0,5 ug / mL; BHT2, 1 ug / mL; BHT3, 1,5 ug / mL) ou DMSO (DMSO3, 0,375 uL de DMSO por ml; DMSO4, 1,125 uL de DMSO / mL) foram adicionados à o extensor. Após a diluição, o sêmen foi dividido em duas alíquotas. O primeiro foi resfriado a 5 ° C por 24h, enquanto o segundo foi congelado. Os parâmetros de motilidade espermática e integridade da membrana plasmática (PMI) foram avaliados, imediatamente após a diluição (0h) e 24h após o resfriamento e nas amostras congeladas, pelo CASA e microscopia de epifluorescência, respectivamente. A inclusão de quercetina ou BHT não afetou os parâmetros de motilidade espermática e PMI de espermatozoides frescos, resfriados ou congelados (P < 0,05). Portanto, a inclusão de quercetina e BHT não beneficiou os parâmetros espermáticos do sêmen ovino submetido a armazenamento líquido a 5 ° C por 24h ou protocolo de congelamento no presente estudo. No entanto, mais estudos são necessários para testar o efeito desses antioxidantes na fertilidade do sêmen ovino criopreservado.

Published
2022

45. Can blood progesterone concentration identify non‐pregnant buffaloes to support estrus resynchronization?

Author

Codognoto, Viviane M., primary, Souza, Fabiana F., additional, Salgado, Letícia C., additional, Rizzoto, Guilherme, additional, Yamada, Paulo H., additional, Marques, Nayara F. S., additional, Carvalho, Nélcio A. T., additional, Dantas, Ariane, additional, Mesquita, Ana Victória Pereira, additional, Ferreira, João C. P., additional, and Oba, Eunice, additional

Published
2022
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46. Modelo anatômico de sistema circulatório da glândula mamária de ovinos como ferramenta didática em aulas de ciências no ensino médio da rede pública / Anatomical model of the circulatory system of the ovine breast gland as a teaching tool in science classes in high school in the public network

Author

Gimenes, Ana Beatriz Batisoco Quessada, primary, Ferreira, Mariah Eduarda, additional, Silva, Marcos Vinícius Ramos da, additional, Leal, Rodrigo, additional, Souza, Estevam Rodrigues de, additional, Dantas, Ariane, additional, Filadelpho, André Luís, additional, and Oba, Eunice, additional

Published
2022
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