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In order to study the interaction of organic matter with arsenic (As) and lead (Pb) in natural water, standard dissolved organic matter (standard DOM) was taken as the research object, and the analysis was carried out by spectroscopy, high-resolution mass spectrometry, parallel factor analysis and other methods. The results showed that compared with fulvic acids and humus, protein-like tyrosines showed higher binding capacity to As and Pb. The results of high-resolution mass spectrometry showed that heavy metals could change the original characteristics of the standard DOM, resulting in the transformation of organic matter, such as the decrease of amino carbohydrate components, the increase of tannin-like components, the decrease of the relative abundance of CHO, and the increase of the relative abundance of other heteroatoms. DOM-As showed that there was a strong positive correlation between the ultraviolet characteristic parameter a254 and the fluorescence characteristic parameter HIX, the amino sugar component and CHO obtained by ultra-high resolution mass spectrometry were positively correlated with the ultraviolet characteristic parameters a254, a260, a280 and the fluorescence characteristic parameter HIX, and the fluorescence component C1 was positively correlated with the unsaturated hydrocarbon component obtained by mass spectrometry. The results of the study may provide theoretical support for the quantitative assessment of the mechanism of organic matter-heavy metal interactions in water bodies, thereby reducing the environmental behavior of various pollutants such as heavy metals. [ABSTRACT FROM AUTHOR]

Imatinib, the prototype BCR‐ABL tyrosine kinase inhibitor (TKI), is the first‐line treatment for Philadelphia chromosome‐positive chronic myeloid leukemia (CML) in the chronic phase. However, a subgroup of patients exhibit poor response or experience relapse. This issue may be overcome by combination therapy using natural compounds. Neferine, a major bisbenzylisoquinoline alkaloid extracted from "lotus plumule" (seed embryo of lotus) commonly used in traditional Chinese medicine and tea, was used herein in the combination treatment of CML. The MTT assay showed that neferine exerted cytotoxicity in primary CML cells in a dose‐dependent manner. Moreover, low concentrations of neferine (4 and 8 µM) sensitized primary CML cells to imatinib (CI < 1), and significantly decreased its IC50 from 0.70 ± 0.10 to 0.32 ± 0.06 µM and 0.16 ± 0.02 µM, respectively. Cotreatment of neferine and imatinib significantly decreased the expression of BCR‐ABL protein and its molecular chaperone heat shock protein 90 (Hsp90) mRNA and protein levels, and further decreased phospho‐extracellular regulated protein kinase 1/2 (p‐Erk1/2) and myeloid cell leukemia (Mcl‐1) expression. These results suggest that neferine might be a potential imatinib sensitizer in CML treatment. Practical application: In China, Lotus plumule, the green embryo of lotus, is used as a tea and as a source of herbal medicine in the treatment of anxiety, insomnia, spermatorrhea, and thirst. Additional, neferine, a bisbenzylisoquinoline alkaloid extracted from lotus plumule has been shown to have antitumor potential. Herein, the effect of neferine and imatinib cotreatment on primary CML cells obtained from CML patients was assessed, with a synergistic effect being observed between the two compounds. Therefore, neferine might be a promising natural compound to potentiate imatinib in CML patients. [ABSTRACT FROM AUTHOR]

The aim of this study is to investigate the risk factors of diabetic foot ulcer (DFU) in patients with Type 2 diabetes. Baseline characteristics of DFU-free patients with Type 2 diabetes were retrospectively collected and DFU was identified during the follow-up. Incidence of DFU was calculated and cumulative incidence was estimated by Kaplan-Meier method. Cox regression model was used to explore factors associated with DFU. A total of 980 patients were included with a median follow-up time of 28.7 months. 259 (26.4%) patients developed DFU with an incidence rate of 11.3 per 100 person-years. The cumulative incidences of DFU at 1 year and 2 years during the follow-up were 5.4% (95% CI 3.9-6.9%) and 14.1% (95% CI 11.7-16.5%), respectively. Cox regression analysis indicated that factors associated with developing DFU included age (hazard ratio (HR)=1.06, 95% CI 1.05-1.07, per 1-year increase), body mass index (HR=1.05, 95% CI 1.02-1.07), higher level of education (HR=0.77, 95% CI 0.60-0.98), hypertension (HR=1.90, 95% CI 1.47-2.45), hyperlipidemia (HR=2.63, 95% CI 2.02-3.43), coronary heart disease (HR=2.88, 95% CI 2.22-3.75), heart failure (HR=2.47, 95% CI 1.91-3.20), stroke (HR=2.44, 95% CI 1.86-3.19), diabetic retinopathy (HR=1.86, 95% CI 1.40-2.48), diabetic kidney disease (HR=1.89, 95% CI 1.41-2.53), diabetic neuropathy (HR=1.73, 95% CI 1.31-2.30), poor glycemic control (HR=1.13, 95% CI 1.07-1.19, per 1% glycosylated hemoglobin increase), and course of diabetes (HR=1.01, 95% CI 1.00-1.01, per 1-month increase). The results showed a relatively high incidence of DFU, and revealed several baseline characteristics identified as risk factors of developing DFU., Competing Interests: None., (AJTR Copyright © 2021.)

Objective: To investigate the effect of vacuum sealing drainage (VSD) after early woundabrasion (WA) in diabetic patients with deep second degree burn (DSDB)., Methods: This prospective study selected 89 diabetic patients with DSDB treated in our hospital. Using the random number table method, the patients were divided into the control group (44 cases with conventional treatment after early WA) and observation group (45 cases with VSD treatment after WA). Wound healing, fungal infection rate, serum levels of inflammatory factors and vascular endothelial growth factor (VEGF), and adverse reactions in both groups were compared., Results: After 2 weeks of treatment, the total effective rate of observation group was higher than that of control group, and the incidence of adverse reactions showed an opposite trend (both P<0.05). Compared with the control group, the wound healing time was shortened, the wound healing rate was increased, and the fungal infection rate was decreased in the observation group (all P<0.05). Compared with before treatment, serum TNF-α levels in both groups were decreased, while serum levels of IL-10, IL-4 and VEGF were increased after treatment, and the changes in the observation group were more obvious (all P<0.05)., Conclusions: In diabetic patients with DSDB, VSD after early WA can effectively reduce the incidence of fungal infection, reduce inflammation, improve VEGF level, and facilitate wound healing., Competing Interests: None., (AJTR Copyright © 2021.)

Phosphorus (P) is an essential nutrient element for biological growth that can contribute to eutrophication in aquatic ecosystems. Water trophic status and algae growth are primarily related to the content of bioavailable P, which is primarily related to enzymatically hydrolysable organic P(EHOP) and dissolved inorganic P(IP). In this study, soil samples from the water-level fluctuation zone (WLFZ) were collected from a tributary of the Three Gorges Reservoir (TGR) to characterize the properties of organic P(OP) fractions using solution 31 P-nuclear magnetic resonance (NMR) and enzymatic hydrolysis. 31 P-NMR showed that orthophosphate was the main part of the bioavailable P in the WLFZ soil and accounted for 80.4% of the NaOH-EDTA extractable total P (NaOH-EDTA TP), while phosphate monoester accounted for 60.5% of NaOH-EDTA extractable OP (NaOH-EDTA OP). The soil properties and replenishment from the mainstream of the Yangtze River to the Pengxi River have a certain effect on the content and distribution of P forms in the WLFZ soil of the tributary. The EHOP accounted for 28.1% of the NaOH-EDTA OP, and a significant positive correlation was observed between labile monoester P and EHOP and organic matter (OM). The water-soluble OP(H 2 O-OP), bicarbonate-extractable OP(NaHCO 3 -OP), and Fe- and Al-associated OP(Fe/Al-OP) were significantly hydrolyzed by phosphatase and thus exhibited great release potential. The ranking of the bioavailability of OP was Fe/Al-OP > H 2 O-OP > NaHCO 3 -OP. Phytate-like P were mainly found in H 2 O-OP and NaHCO 3 -OP, which indicated that periodic submersion-emersion cycles promoted the release of phytate-like P from Fe/Al-OP into the water column of the TGR. These observations suggest that when the external P input was effectively controlled, a huge risk of release of the internal OP from the WLFZ soil, and the biogeochemical cycling of the bioavailable P played an important role in maintaining the eutrophication of the reservoir., (Copyright © 2019 Elsevier Ltd. All rights reserved.)