1. Colorimetric Sensor Array for Simultaneous Assay of Catecholamine Neurotransmitters Using Various-Sized Citrate-Capped Gold Nanoparticles.
- Author
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Mohseni, Naimeh and Bahram, Morteza
- Abstract
AbstractDriven by the need to
in situ monitoring of the endogenous substances in biological matrices, a simple, rapid and sensitive optical nanoarray system has been designed for determination and visual discrimination of four important catecholamine neurotransmitters: dopamine (DA), epinephrine (EP), norepinephrine (NE) and levodopa (LD). To fabricate this platform, spherical citrate-capped gold nanoparticles (AuNPs) in the size range of 13–42 nm have been employed as sensing elements. For each catecholamine different responses toward the AuNPs was obtained indicating unique aggregation kinetics. When the sensor array exposed to different catecholamines (CAs), both the color and UV–Vis absorbance spectra changed due to the interaction of analytes and AuNPs, creating a unique fingerprint-like pattern for each analyte which can be visually detected by naked eye. To analyze response profiles, we employed unsupervised chemometric algorithms including hierarchical cluster analysis (HCA) and principal component analysis (PCA) and satisfactory class separations were achieved. Furthermore, the sensitive RGB based color difference patterns were generated providing easily monitored visual output. Based on this sensor array, the set of four catecholamine neurotransmitters have been correctly identified and classified in the spiked real urine samples. The target analytes have been well distinguished at low concentrations of 0.3 − 4 μM DA, 0.8 − 10 μM EP, 0.8 − 8 μM NE, and 0.3 − 10 μM LD using the proposed “chemical nose” strategy. We believe that the established optical sensor array can be potentially employed as an efficient discrimination tool for diagnostic and therapeutic applications in the future. [ABSTRACT FROM AUTHOR]- Published
- 2024
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