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The first observations of the heat release reaction in the nickel-lithium-hydrogen mixture of the A. Rossi heat generator were carried out at the Institute for Nuclear Research of the National Academy of Sciences of Ukraine. The presence of this reaction was recorded using the difference of the e.m.f. thermocouples in two separate reactor chambers with “fuel” and without it, which were inside of the heater. “Fuel” was a nickel powder with 10 % lithium aluminum hydride, total weight of 100 mg. At the first start-up of reactor with “fuel”, the reaction occurs at the temperature of 950 °C and intensively develops up to 1050 °C. Difference e.m.f. thermocouples in two reactor chambers does not exceed 20 °C. For 600 h of observation of this reaction, its behavior was ascertained: under repeated starts, the reaction begins at the temperature of less than 700 °C; the radiation power of gamma quanta with energy above 50 keV, which accompanies this reaction, is less than 0.012 µR/h; the neutron flux in 4π composes 6000 neutrons/h (±40 %); there was change in the isotope composition of 6Li from 7 % for “fuel” to 12 % for “ash”. For nickel isotopes, the decrease in the composition of 60Ni by 2.5 % and the increase in the composition of 62Ni at 0.5 % is indicated. This behavior certainly indicates that a low-energy nuclear reaction (LENR) is observed.