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Your search keyword '"O. Alarcia‐Alejos"' showing total 4 results
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4 results on '"O. Alarcia‐Alejos"'

1. Presence of Toxoplasma gondii and Neospora caninum DNA in the brain of wild birds

Author

Laila Darwich, O. Alarcia-Alejos, Rafael A. Molina-López, I. Echeverria, M. Pabón, Ignasi Marco, Sonia Almería, Oscar Cabezón, Santiago Lavín, and Fernando López-Gatius

Subjects
Polymerase Chain Reaction, Birds, Neospora, Species Specificity, biology.animal, parasitic diseases, Prevalence, Animals, Bird Diseases, Milvus migrans, General Veterinary, biology, Coccidiosis, Brain, Toxoplasma gondii, Sequence Analysis, DNA, General Medicine, Buteo buteo, DNA, Protozoan, biology.organism_classification, Virology, Neospora caninum, Buzzard, Toxoplasmosis, Animal, Spain, Parasitology, Gyps fulvus, Toxoplasma
Abstract

Toxoplasma gondii infections are prevalent in many avian species and can cause mortality in some bird hosts. Although T. gondii has been isolated from various species of birds, the role of many different species of wild birds in the epidemiology of T. gondii remains unknown. Neospora caninum, a closely related parasite to T. gondii, has been recently confirmed to infect domestic chickens and wild birds such as house sparrows (Passer domesticus). The present study reports the presence of T. gondii and N. caninum DNA by PCR in brain tissues of 14 species of wild birds from Spain. From a total of 200 samples analyzed, 12 samples (6%) were positive for T. gondii [5 Eurasian jays (Garrulus glandarius), 5 magpies (Pica pica), 1 black kite (Milvus migrans) and 1 Griffon vulture (Gyps fulvus)], while 3 samples (1.5%) were positive for N. caninum [2 magpies and 1 common buzzard (Buteo buteo)]. This is the first report of detection of T. gondii in magpies, griffon vulture and black kite and of N. caninum in common buzzard and magpies, extending the list of natural intermediate hosts for T. gondii and N. caninum infections to these species.

Published
2012
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2. Influenza A Virus Surveillance in the Invasive American Mink (Neovison vison) from Freshwater Ecosystems, Northern Spain

Author

A. Sánchez, María P. Ribas, N. Busquets, P. Lizarraga, O. Alarcia‐Alejos, Xavier Fernández-Aguilar, Hojjat Gholipour, G. De Pedro, C. Temiño, and Oscar Cabezón

Subjects
0301 basic medicine, Male, Time Factors, Epidemiology, Range (biology), media_common.quotation_subject, 030106 microbiology, Orthomyxoviridae, Zoology, Biology, medicine.disease_cause, Competition (biology), Neovison, 03 medical and health sciences, Orthomyxoviridae Infections, Zoonoses, Influenza A virus, medicine, Animals, Carnivore, American mink, Ecosystem, media_common, General Veterinary, General Immunology and Microbiology, Public Health, Environmental and Occupational Health, biology.organism_classification, 030104 developmental biology, Infectious Diseases, Mink, Spain, biology.protein, Female, Antibody, Introduced Species
Abstract

Influenza A viruses (IAVs) are negative-sense, single-stranded and segmented RNA viruses of the Orthomyxoviridae family that may cause acute respiratory disease in a wide range of birds and mammals. Susceptibility of several species within the family Mustelidae to IAVs has been reported as a result of natural or experimental infections. The objectives of this study were to assess whether free-ranging American mink populations from Northern Spain were infected with IAV and try to define the role of this species in the epidemiology of IAV. Sera from 689 American mink from Northern Spain captured between 2011 and 2014 were tested for the presence of antibodies against IAVs using a commercial competition cELISA. Positive sera were further analysed with haemagglutination inhibition (HI) assay. Fifteen of the 689 (2.2%, 1.3-3.6 CI95% ) of the American minks analysed were ELISA positive. No significant differences were observed between years of capture, provinces, river basins, sexes or ages of the animals. All seropositive sera resulted negative to the panel strains used in the HI assay, showing that the most relevant strains circulating in swine, the most relevant avian subtypes (H5 and H7) and the H10N4 subtype isolated in minks have not been circulating in this free-ranging exotic carnivore from Spain. In the light of these results, the free-range American mink from Northern Spain do not seem to have an important role in the epidemiology of IAVs.

Published
2016

3. Tracking Toxoplasma gondii in freshwater ecosystems: interaction with the invasive American mink (Neovison vison) in Spain.

Author

Ribas MP, Almería S, Fernández-Aguilar X, De Pedro G, Lizarraga P, Alarcia-Alejos O, Molina-López R, Obón E, Gholipour H, Temiño C, Dubey JP, and Cabezón O

Subjects
Agglutination Tests veterinary, Animals, Antibodies, Protozoan blood, Ecosystem, Fresh Water, Male, Seroepidemiologic Studies, Spain epidemiology, Toxoplasma genetics, Toxoplasmosis, Animal parasitology, Mink parasitology, Toxoplasma isolation & purification, Toxoplasmosis, Animal epidemiology, Toxoplasmosis, Animal transmission
Abstract

Water-borne transmission may play an important role in the epidemiology of Toxoplasma gondii. Mammals closely related to freshwater ecosystems, such as the American mink (Neovison vison), are potentially valuable sentinels for T. gondii. To assess the importance of freshwater ecosystems in T. gondii epidemiology, sera of 678 American minks collected during the 2010 to 2015 Spanish national eradication campaigns were tested for the presence of T. gondii antibodies using the modified agglutination test (MAT, cut-off 1:25). A high prevalence of samples, 78.8% (CI 95% : 75.5-81.8), were seropositive. In addition, a specific real-time PCR was performed in 120 brain samples and the parasite DNA was detected in 9.2% (CI 95% : 5.2-15.7). Significant differences in seroprevalence were detected among bioregions, with the highest levels detected in coastal areas, and by age. The higher seroprevalence observed in older animals (80.0% adults versus 68.7% juveniles) confirms the importance of the horizontal transmission. These results indicate a widespread presence of T. gondii oocysts in freshwater ecosystems from Spain and further support the importance of water-borne transmission in the epidemiology of T. gondii.

Published
2018
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4. Influenza A Virus Surveillance in the Invasive American Mink (Neovison vison) from Freshwater Ecosystems, Northern Spain.

Author

Gholipour H, Busquets N, Fernández-Aguilar X, Sánchez A, Ribas MP, De Pedro G, Lizarraga P, Alarcia-Alejos O, Temiño C, and Cabezón O

Subjects
Animals, Female, Male, Orthomyxoviridae Infections epidemiology, Orthomyxoviridae Infections virology, Spain epidemiology, Time Factors, Zoonoses, Ecosystem, Influenza A virus isolation & purification, Introduced Species, Mink virology, Orthomyxoviridae Infections veterinary
Abstract

Influenza A viruses (IAVs) are negative-sense, single-stranded and segmented RNA viruses of the Orthomyxoviridae family that may cause acute respiratory disease in a wide range of birds and mammals. Susceptibility of several species within the family Mustelidae to IAVs has been reported as a result of natural or experimental infections. The objectives of this study were to assess whether free-ranging American mink populations from Northern Spain were infected with IAV and try to define the role of this species in the epidemiology of IAV. Sera from 689 American mink from Northern Spain captured between 2011 and 2014 were tested for the presence of antibodies against IAVs using a commercial competition cELISA. Positive sera were further analysed with haemagglutination inhibition (HI) assay. Fifteen of the 689 (2.2%, 1.3-3.6 CI 95% ) of the American minks analysed were ELISA positive. No significant differences were observed between years of capture, provinces, river basins, sexes or ages of the animals. All seropositive sera resulted negative to the panel strains used in the HI assay, showing that the most relevant strains circulating in swine, the most relevant avian subtypes (H5 and H7) and the H10N4 subtype isolated in minks have not been circulating in this free-ranging exotic carnivore from Spain. In the light of these results, the free-range American mink from Northern Spain do not seem to have an important role in the epidemiology of IAVs., (© 2016 Blackwell Verlag GmbH.)

Published
2017
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