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5 results on '"O’Brien, Gilbert"'

1. Using cellular fitness to map the structure and function of a major facilitator superfamily effluxer.

Author

Perez, Anisha M, Gomez, Marcella M, Kalvapalle, Prashant, O'Brien-Gilbert, Erin, Bennett, Matthew R, and Shamoo, Yousif

Subjects
Bacterial Proteins, Membrane Transport Proteins, Kinetics, Multigene Family, Models, Biological, antibiotic resistance, efflux pump, major facilitator superfamily, structure function, tetracycline, Models, Biological, Bioinformatics, Biochemistry and Cell Biology, Other Biological Sciences
Abstract

The major facilitator superfamily (MFS) effluxers are prominent mediators of antimicrobial resistance. The biochemical characterization of MFS proteins is hindered by their complex membrane environment that makes in vitro biochemical analysis challenging. Since the physicochemical properties of proteins drive the fitness of an organism, we posed the question of whether we could reverse that relationship and derive meaningful biochemical parameters for a single protein simply from fitness changes it confers under varying strengths of selection. Here, we present a physiological model that uses cellular fitness as a proxy to predict the biochemical properties of the MFS tetracycline efflux pump, TetB, and a family of single amino acid variants. We determined two lumped biochemical parameters roughly describing Km and Vmax for TetB and variants. Including in vivo protein levels into our model allowed for more specified prediction of pump parameters relating to substrate binding affinity and pumping efficiency for TetB and variants. We further demonstrated the general utility of our model by solely using fitness to assay a library of tet(B) variants and estimate their biochemical properties.

Published
2017

2. Letters.

Author

Omand, David, Norris, Emma, Accurate, Fairly, Boyle, Nicholas, Belton, Teresa, Eagles, Bishop Peter, Wright, Stephen, Kemp, Ian, Day, Nigel, Dyer, John, and O’Brien, Gilbert

Abstract

The article discusses various topics, including the use of spyware by foreign powers to surveil dissidents, the limitations of public inquiries in holding those in power accountable, campus free speech issues, reforming the House of Lords, addressing climate change, the Old Catholic Churches, the concept of forgiveness in politics, accurate science reporting, the state of the county court system, experiences in prep and public schools, the language used to discuss problems with the UK's water services, and temporary accommodation in the UK. The article also includes several unrelated statistics and facts. [Extracted from the article]

Published
2024

3. Using cellular fitness to map the structure and function of a major facilitator superfamily effluxer

Author

Matthew R. Bennett, Prashant Kalvapalle, Anisha M Perez, Erin O'Brien‐Gilbert, Marcella M. Gomez, and Yousif Shamoo

Subjects
0301 basic medicine, antibiotic resistance, Bioinformatics, 1.1 Normal biological development and functioning, major facilitator superfamily, 030106 microbiology, Bioengineering, Computational biology, macromolecular substances, Biology, Models, Biological, Article, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Bacterial Proteins, Underpinning research, Models, structure function, Pharmacology & Drug Discovery, Single amino acid, Quantitative Biology & Dynamical Systems, Organism, tetracycline, General Immunology and Microbiology, Applied Mathematics, Structure function, Membrane Transport Proteins, Articles, Biological, Microbiology, Virology & Host Pathogen Interaction, Major facilitator superfamily, Structure and function, Physiological model, Kinetics, 030104 developmental biology, Computational Theory and Mathematics, Multigene Family, efflux pump, Generic health relevance, Efflux, Biochemistry and Cell Biology, Other Biological Sciences, General Agricultural and Biological Sciences, Information Systems
Abstract

The major facilitator superfamily (MFS) effluxers are prominent mediators of antimicrobial resistance. The biochemical characterization of MFS proteins is hindered by their complex membrane environment that makes in vitro biochemical analysis challenging. Since the physicochemical properties of proteins drive the fitness of an organism, we posed the question of whether we could reverse that relationship and derive meaningful biochemical parameters for a single protein simply from fitness changes it confers under varying strengths of selection. Here, we present a physiological model that uses cellular fitness as a proxy to predict the biochemical properties of the MFS tetracycline efflux pump, TetB, and a family of single amino acid variants. We determined two lumped biochemical parameters roughly describing K m and V max for TetB and variants. Including in vivo protein levels into our model allowed for more specified prediction of pump parameters relating to substrate binding affinity and pumping efficiency for TetB and variants. We further demonstrated the general utility of our model by solely using fitness to assay a library of tet(B) variants and estimate their biochemical properties.

Published
2017

4. Puzzled Puss.

Author

O'Brien, Gilbert and Rose, Peter

Subjects
*ACTORS, *MUSICALS
Published
2023

5. Around Here.

Author

O'Brien, Gilbert and Towner, Paul

Subjects
MONTAGUE Street (New York, N.Y.)
Abstract

Several letters to the editor are presented in response to the article by Alice Spawls in the February 4, 2016 issue that discusses information related to the Montague Street, New York.

Published
2016

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