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457 results on '"O'Malley, Dennis"'

2. DNA mismatch repair defect and intratumor heterogeneous deficiency differently impact immune responses in diffuse large B-cell lymphoma.

Author

Xu-Monette, Zijun Y., Luo, Cancan, Yu, Li, Li, Yong, Bhagat, Govind, Tzankov, Alexandar, Visco, Carlo, Fan, Xiangshan, Dybkaer, Karen, Sakhdari, Ali, Wang, Nicholas T., Yuan, Alyssa F., Chiu, April, Tam, Wayne, Zu, Youli, Hsi, Eric D., Perry, Anamarija M., Song, Wenting, O'Malley, Dennis, and Au, Qingyan

Subjects
DIFFUSE large B-cell lymphomas, GENE expression, GENE expression profiling, T cells, GENE silencing, DNA mismatch repair
Abstract

Deficient (d) DNA mismatch repair (MMR) is a biomarker predictive of better response to PD-1 blockade immunotherapy in solid tumors. dMMR can be caused by mutations in MMR genes or by protein inactivation, which can be detected by sequencing and immunohistochemistry, respectively. To investigate the role of dMMR in diffuse large B-cell lymphoma (DLBCL), MMR gene mutations and expression of MSH6, MSH2, MLH1, and PMS2 proteins were evaluated by targeted next-generation sequencing and immunohistochemistry in a large cohort of DLBCL patients treated with standard chemoimmunotherapy, and correlated with the tumor immune microenvironment characteristics quantified by fluorescent multiplex immunohistochemistry and gene-expression profiling. The results showed that genetic dMMR was infrequent in DLBCL and was significantly associated with increased cancer gene mutations and favorable immune microenvironment, but not prognostic impact. Phenotypic dMMR was also infrequent, and MMR proteins were commonly expressed in DLBCL. However, intratumor heterogeneity existed, and increased DLBCL cells with phenotypic dMMR correlated with significantly increased T cells and PD-1+ T cells, higher average nearest neighbor distance between T cells and PAX5+ cells, upregulated immune gene signatures, LE4 and LE7 ecotypes and their underlying Ecotyper-defined cell states, suggesting the possibility that increased T cells targeted only tumor cell subsets with dMMR. Only in patients with MYC¯ DLBCL, high MSH6/PMS2 expression showed significant adverse prognostic effects. This study shows the immunologic and prognostic effects of genetic/phenotypic dMMR in DLBCL, and raises a question on whether DLBCL-infiltrating PD-1+ T cells target only tumor subclones, relevant for the efficacy of PD-1 blockade immunotherapy in DLBCL. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Practical Applications in Immunohistochemistry: An Immunophenotypic Approach to the Spleen

Author

Borch, William R., Aguilera, Nadine S., Brisette, Mark D., O'Malley, Dennis P., and Auerbach, Aaron

Subjects
Usage, B cells, Immunohistochemistry, Lymphomas, World health, Tumors, Public health
Abstract

Despite the routine use of immunohistochemistry as a tool to assist in the diagnosis of surgical pathology and hematopathology specimens, immunohistochemical evaluation of the spleen remains confusing for many. Unusual [...], * Context.--Even though immunohistochemistry is routinely used by pathologists, evaluation of immunohistochemistry in splenic lesions remains difficult for many. Classification of benign and splenic lesions often requires a combination of hematoxylin-eosin evaluation, immunophenotyping, and sometimes molecular testing. Immunohistochemical staining is essential in evaluating many splenic lesions, and requires an understanding of the normal compartments of the spleen. Objective.--To address different immunohistochemical features used for identification and subclassification of different lesions of the spleen, as well as in the normal compartments of the spleen. Data Sources.--The information outlined in this review article is based on our experiences with a variety of spleen cases, on the current World Health Organization classification of hematopoietic and lymphoid tumors, and on a review of English-language articles published during 2018. Conclusions.--Features for phenotyping normal spleen as well as a variety of splenic lesions, including littoral cell angioma and splenic marginal zone lymphoma, are discussed. Suggested immunopanels are provided to assist in the diagnosis of different lesions of the spleen. (Arch Pathol Lab Med. 2019;143:1093-1105; doi: 10.5858/arpa.2018-0211-CP)

Published
2019
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12. Predictive Markers Require Thorough Analytic Validation

Author

Troxell, Megan L., Fulton, Regan S., Swanson, Paul E., Bellizzi, Andrew M., Fitzgibbons, Patrick L., Ambaye, Abiy B., Haas, Thomas S., Goldsmith, Jeffrey D., Loykasek, Patti A., Miller, Dylan V., O'Malley, Dennis, Qiu, Jingxin, Salama, Mohamed E., Schaberg, Kurt B., Schwartz, Robert A., Shia, Jinru, Summers, Thomas A., Jr., and Wu, Yaping

Subjects
United States. Food and Drug Administration, Immunohistochemistry, Non-small cell lung cancer, Small cell lung cancer
Abstract

To the Editor. -- The recent editorial by Erik Thunnissen, 'How to Validate Predictive Immunohistochemistry Testing in Pathology? A Practical Approach Exploiting the Heterogeneity of Programmed Death Ligand-1 Present in [...]

Published
2019

18. Bone Marrow

Author

O’Malley, Dennis P., Fedoriw, Yuri, Cheng, Liang, editor, and Bostwick, David G., editor

Published
2016
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19. Spleen

Author

O’Malley, Dennis P., Fedoriw, Yuri, Cheng, Liang, editor, and Bostwick, David G., editor

Published
2016
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31. Prognostic and biological significance of survivin expression in patients with diffuse large B-cell lymphoma treated with rituximab-CHOP therapy

Author

Liu, Zhiyu, Xu-Monette, Zijun Y, Cao, Xin, Manyam, Ganiraju C, Wang, Xiaoxiao, Tzankov, Alexandar, Xia, Yi, Li, Xin, Visco, Carlo, Sun, Ruifang, Zhang, Li, Montes-Moreno, Santiago, Dybkær, Karen, Chiu, April, Orazi, Attilio, Zu, Youli, Bhagat, Govind, Richards, Kristy L, Hsi, Eric D, Choi, William WL, van Krieken, J Han, Huh, Jooryung, Ponzoni, Maurilio, Ferreri, Andrés JM, Parsons, Ben M, Møller, Michael B, Piris, Miguel A, Winter, Jane N, O'Malley, Dennis P, Medeiros, L Jeffrey, and Young, Ken H

Published
2015
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34. Spleen

Author

O’Malley, Dennis P., Orazi, Attilio, Cheng, Liang, editor, and Bostwick, David G., editor

Published
2011
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35. Bone Marrow

Author

O’Malley, Dennis P., Cheng, Liang, editor, and Bostwick, David G., editor

Published
2011
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40. Contributors

Author

Anastasi, John, primary, Arber, Daniel A., additional, Chen, Dong, additional, Chikwava, Kudakwashe, additional, Choi, John Kim, additional, Cook, James R., additional, Fedoriw, Yuri, additional, Fend, Falko, additional, Ferry, Judith A., additional, Finn, William G., additional, Gao, Juehua, additional, George, Tracy I., additional, Hasserjian, Robert Paul, additional, He, Rong, additional, Hoyer, James D., additional, Hsi, Eric D., additional, King, Rebecca L., additional, Kottke-Marchant, Kandice, additional, Kurtin, Paul J., additional, Kussick, Steven J., additional, Lin, Pei, additional, Macon, William R., additional, Monaghan, Sara A., additional, Nakashima, Megan O., additional, Nguyen, Phuong L., additional, Ohgami, Robert S., additional, Oliveira, Jennifer L., additional, O'Malley, Dennis P., additional, Picarsic, Jennifer Lee, additional, Quintanilla-Martinez, Leticia, additional, Rogers, Heesun J., additional, Said, Jonathan W., additional, Slack, Graham W., additional, Smith, Lauren, additional, Thakral, Beenu, additional, Viswanatha, David S., additional, Wang, Sa A., additional, and Ziai, James M., additional

Published
2018
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42. The implication of identifying JAK2 V617F in myeloproliferative neoplasms and myelodysplastic syndromes with bone marrow fibrosis

Author

Olsen, Randall, Dunphy, Cherie, O’Malley, Dennis, Rice, Lawrence, Ewton, April, and Chang, Chung-Che

Abstract

Abstract: The myeloproliferative neoplasms (MPN) and myelodysplastic syndromes (MDS) occasionally demonstrate overlapping morphological features including hypercellularity, mild/nonspecific dysplastic changes and variable bone marrow fibrosis. Thus, when the associated bone marrow fibrosis results in a suboptimal specimen for morphological evaluation, the descriptive diagnosis “fibrotic marrow with features indeterminate for MDS versus MPN” is often applied. The JAK2 V617F mutation was recently shown to be frequently identified in MPN, but it is rarely present in other myeloid disorders. However, the diagnostic utility of JAK2 V617F screening in hypercellular bone marrow specimens with fibrosis has not been previously investigated. Using a real-time polymerase chain reaction melting-curve assay capable of detecting JAK2 V617F in archived fixed materials, we retrospectively studied JAK2 V617F in 45 cases with fibrotic hypercellular bone marrow at initial presentation, including 19 cases initially described as “with features indeterminate for MDS versus MPN”. These 19 cases were reclassified into more specific categories of MDS (n = 14) or MPN (n = 5) based on the availability of subsequent clinical data and/or bone marrow examinations. The JAK2 V617F allele was identified in 17 out of 18 BCR/ABL gene-negative MPN cases with marrow fibrosis, whereas only wild-type alleles were identified in the remaining non-MPN cases. Importantly, JAK2 V617F alleles were seen in all five cases of “with features indeterminate for MDS versus MPN” at initial presentation that were later determined to be MPN, but they were absent in the 14 cases later determined to be MDS. Our results suggest that JAK2 V617F allele evaluation can be a useful ancillary test for discriminating MDS from MPN in specimens with bone marrow fibrosis.

Published
2024
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50. Template for Reporting Results of Monitoring Tests for Patients With Chronic Myelogenous Leukemia (BCR-[ABL1.sup.+])

Author

Kelley, Todd W., Arber, Daniel A., Gibson, Christine, Jones, Daniel, Khoury, Joseph D., Medeiros, Bruno C., O'Malley, Dennis P., Patel, Keyur P., Pilichowska, Monika, Vasef, Mohammad A., Wallentine, Jeremy, and Zehnder, James L.

Subjects
Diagnosis, Care and treatment, Reports, Patient monitoring equipment -- Reports
Abstract

The College of American Pathologists (CAP) offers these templates to assist pathologists in providing clinically useful and relevant information when reporting results of biomarker testing. The CAP regards the reporting [...]

Published
2016
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