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1. Point-of-Care Approaches for Meningitis Diagnosis in a Low-Resource Setting (Southwestern Uganda): Observational Cohort Study Protocol of the 'PI-POC' Trial

Author

Gaudenzi, Giulia, Kumbakumba, Elias, Rasti, Reza, Nanjebe, Deborah, Réu, Pedro, Nyehangane, Dan, Mårtensson, Andreas, Nassejje, Milly, Karlsson, Jens, Mzee, John, Nilsson, Peter, Businge, Stephen, Loh, Edmund, Boum II, Yap, Andersson-Svahn, Helene, Gantelius, Jesper, Mwanga-Amumpaire, Juliet, and Alfvén, Tobias

Subjects
Medicine, Computer applications to medicine. Medical informatics, R858-859.7
Abstract

BackgroundA timely differential diagnostic is essential to identify the etiology of central nervous system (CNS) infections in children, in order to facilitate targeted treatment, manage patients, and improve clinical outcome. ObjectiveThe Pediatric Infection-Point-of-Care (PI-POC) trial is investigating novel methods to improve and strengthen the differential diagnostics of suspected childhood CNS infections in low-income health systems such as those in Southwestern Uganda. This will be achieved by evaluating (1) a novel DNA-based diagnostic assay for CNS infections, (2) a commercially available multiplex PCR-based meningitis/encephalitis (ME) panel for clinical use in a facility-limited laboratory setting, (3) proteomics profiling of blood from children with severe CNS infection as compared to outpatient controls with fever yet not severely ill, and (4) Myxovirus resistance protein A (MxA) as a biomarker in blood for viral CNS infection. Further changes in the etiology of childhood CNS infections after the introduction of the pneumococcal conjugate vaccine against Streptococcus pneumoniae will be investigated. In addition, the carriage and invasive rate of Neisseria meningitidis will be recorded and serotyped, and the expression of its major virulence factor (polysaccharide capsule) will be investigated. MethodsThe PI-POC trial is a prospective observational study of children including newborns up to 12 years of age with clinical features of CNS infection, and age-/sex-matched outpatient controls with fever yet not severely ill. Participants are recruited at 2 Pediatric clinics in Mbarara, Uganda. Cerebrospinal fluid (for cases only), blood, and nasopharyngeal (NP) swabs (for both cases and controls) sampled at both clinics are analyzed at the Epicentre Research Laboratory through gold-standard methods for CNS infection diagnosis (microscopy, biochemistry, and culture) and a commercially available ME panel for multiplex PCR analyses of the cerebrospinal fluid. An additional blood sample from cases is collected on day 3 after admission. After initial clinical analyses in Mbarara, samples will be transported to Stockholm, Sweden for (1) validation analyses of a novel nucleic acid–based POC test, (2) biomarker research, and (3) serotyping and molecular characterization of S. pneumoniae and N. meningitidis. ResultsA pilot study was performed from January to April 2019. The PI-POC trial enrollment of patients begun in April 2019 and will continue until September 2020, to include up to 300 cases and controls. Preliminary results from the PI-POC study are expected by the end of 2020. ConclusionsThe findings from the PI-POC study can potentially facilitate rapid etiological diagnosis of CNS infections in low-resource settings and allow for novel methods for determination of the severity of CNS infection in such environment. Trial RegistrationClinicalTrials.gov NCT03900091; https://clinicaltrials.gov/ct2/show/NCT03900091 International Registered Report Identifier (IRRID)DERR1-10.2196/21430

Published
2020
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2. Effect of decentralising childhood tuberculosis diagnosis to primary health centre versus district hospital levels on disease detection in children from six high tuberculosis incidence countries: an operational research, pre-post intervention study

Author

Laurence, Borand, Agathe, de Lauzanne, Bunnet, Dim, Seyla, Heang, Sanary, Kaing, Chanty, Keang, Socheat, L.Y., Pichpiseth, Meas, Sovann, Nhoueng, Long, Pring, Vouchleang, Sreng, Song, Yin, Saren, Sovan, Chanvirak, Phan, Chanra, Chreng, Ratha, Khoun, Monicando, Rin, Sophea, Pal, Boraneath, Nang, Rathakrun, Pom, Tan Eang, Mao, Simoy, Chhim, Huot, Touch, Kosal, Suon, Saronn, Chum, Kimhong, Tok, Kimchorn, Pring, Satya, Krouch, Chean, Chok, Sunleng, Seun, Savtey, Phon, Mai, Nang, Kimda, Hun, Vanny, Hong, Dara, Sok, Kosal, Chea, Bunthoeun, Chheang, Rino, Sem, Lam, Lay, Haysan, Say, Pholly, Kem, Sreyphal, Meng, Sokheng, Phorn, Sreyvann, Him, Peakdey, Pheach, Dalai, Kive, Moeur, Sar, Sreydy, Kong, Seyha, Kong, Sreytouch, Yorn, Soam, Tes, Sophal, Kep, Seroeung HENG Thy, Leng, Savorn, Neak, Sim, Seng, Pheakna, Pay, Sithan, Suon, Sophanna, Chan, Dyna, Um, Savuth, Sin, Sam, Phan, Sarim, Kum, Sokheng, Khath, Pong, Phem, Seyha, Sok, Chanty, Ny, Van, Leim, Sereyvuth, Pich, Sengkry, Chheang, Nhin, Eang, Vannareth, Sao, Vannak, Sim, Sopheak, Som, Ney, Pong, Sokha, Van, Sreyleap, Seng, Vanna, Yoeurng, Kakada, Toem, Thida, Keo, Vuochny, Sem, Sophal, Veng, Chanthol, Rin, Vanny, Seang, Kiri, Lok, Khemra, Mao, Keovanna, Ouk, Maiya, Min, Suomun, Morm, Rattana, Koy, Sreypov, Chhann, Sreytouch, Set, Audrey, Amboua Schouame, Clifford, Babey, Masama, Eden Ngu, Etienne, Guenou, Sylvie, Kwedi Nolna, Douglas, Mbang Massom, Bernard Fortune, Melingui, Nadia, Nga Elomo, Moïse, Mvetumbo, Angeline, Nkembe, Krystel, Ebo, Albert, Kuate Kuate, Michelline, Choupa, Maggi, Mbede, Valerie, Donkeng, Nelly, Kamgaing, Jean-Voisin, Taguebue, Achille, Touha Yannick, Estelle, Abomo Zang, Rosette, Bakoa, Esperance, Mimbouombela Leger, Sabine, Eleme, Gabrielle, Eteme Marie, Thierry, Elouna Nkoa, Dieudonné, Ndzana, Christophe, Bitti, Olivier, Fotsing, Charlotte, Lani Boko, Mathieu, Mbonga, Ghislain Ulrich, Njakou Sagang, Innocent, Onomo, Charlotte, Essaga Hortense, Daniel Desiré, Mboudi Kouang, Léon, Eloundou, Helene, Kengne, Felix, Mbassi, Juliette, Bidjeme, Hortense, Toua Eteme, Nadege, Essama, Roger, Belinga Balla, Norbert, Tassi, Roline, Nguiko Elsa, Leonard, Mekongo, Fabien, Eyebe Ayissi, Francine Christelle, Biloa Anaba, Philomène, Nsom, Celestin Géraud, Yam Essola, Edwige Léa, Mame Moo, Noé, Makon, Arllette Rita, Nounkep Yanghu, Frank, Ebanga, Antoinette, Assiga Ntsama, Carlyle Sorelle, Kamguia Djuimsop, Diane, Mbabou, Marie, Maguip Abanda, Rosine Berthe, Nguemafouo Doummene, Amos, Mekone, Ngwankfu, Konfor Blessing, Jérome, Mimboe, Virginie, Tiona, Roland, Beleck, Sairou, Zam, Nicole, Adibone, Claude, Biaback Jean, Denis, Bessong, Gilbert, Aminou, Jeremie Pagnol, Bille Bonga, Aubin, Fotso Monkap, Epse NGON Annie, Hitekelek, Vitrice, Sebe, Leo, Makon, Marie, Ennah, Mpie, Paul Boyolo, Diane Viviane, Metchoum, Celin, Nzambe, Arnaud, Dado, Milobert, Mbengang, Marceline, Eyebi, Vanessa, Ngah, Alice, Mballa Batonga, Solange, Ayouba, Pierrette, Ebode, Majino, Mamou, Marguerite, Botomogne Bomba, Salametou, Ngnet, Augustine Florence, Essengue Ngono, Rolland, Odionoloba Charles, Bernice, Bisso, Suzy, Balemaken Ingrid, Marie Louise, Mandoki a Bilong, Gertrude, Ndeng Ayouba, Josue, Ngon, Roger, Aka Bony, Kacou Michel, Bah, Dro, Bakayoko, Rolande, Baki Aimee, Marie-France Larissa, Banga, Olivier, Bouzié, Kan, Brou, Pan, Coulibaly, Serge, Danho, Flavien, Deli, Alphonse, Dion, Bi, Do, Armand, Dohoun, William, Edjeme, Cathérine, Falé, Melissa, Gogoua Saulé, Constant, Kesse, Eric, Komena Auguste, Christian, Kouadio, Arkason, Kouame Abel, Raoul, Moh, Sandrine, Nguessan Marcelle, Bertine, Siloué, Nina, Soua, Cyrille Prisca, Yao Yapi, Timothée, Ouassa, Jacquemin, Kouakou, Eric, Balestre, Aurélie, Beuscart, Aurélie, Charpin, Marc, D'elbee, Hélène, Font, Basant, Joshi, Nicolas, Koskas, Olivier, Marcy, Estelle, Occelli, Joanna, Orne-Gliemann, Julien, Poublan, Elodie, Vernoux, Maryline, Bonnet, Savine, Chauvet, Manon, Lounnas, Guillaume, Breton, Pierre-Yves, Norval, Sheyla, Cassy, Verna, Chambal, Valter, Chiúle, Supinho, Chimbanje, Saniata, Cumbe, Mércia, Matsinhe, Celso, Khosa, Nairo, Mabote, Salvador, Machava, Emelva, Machonisse, Verónica, Macuácua, Denise, Milice, Jorge, Ribeiro, Elcídio, Tivane, Dorlim, Uetela, Yara, Voss de Lima, Américo, Zandamela, Alcina, Zita, Ivan, Manhiça, Benedita, José, Dalila, Rego, Chris, Buck W., Kapoli, Kasembe, Atália, Massangaie, Assa, Sitoe, Ambostique, Argola, Césio, Miambo, Presequila, Nhatsave, Gilda, Sitoe, Charifito, Vesta, Salvador, Dimande, Lázaro, Mazembe, Nilza, Amade, Manuela, Chavela, Nomsa, Macheque, Salomão, Comé, Eulália, Machava, Narciso, Mucavele, Jacinto, Nhabanga, Marlene, Nicolau, Natércia, Simbine, Lina, Uendela, Micaela, Juaio, Abiba, Saíde, Naira, Macie, Fernando, Mondlane, Stélio, Simango, Prince, Beyan, Benjamin M, Flomo, Abubakarr, Jalloh Joseph, Ishmael, Kamara, Monica G, Koroma, Mohamed, Lamin, Lena, Matata, Ross, Mugisha Jacob, Christiana M, Senesie, Sheriff, S.E.S.A.Y., Egerton, Tamba Kamara, Ayeshatu, Mustapha, Lynda, Foray, Sandra, Agondeze, Agnes, Kobusingye, Mastula, Nanfuka, Faith, Namulinda, Eric, Wobudeya, Rinah, Arinaitwe, Rodney, Kaitano, Martin, Kasujja, Juliet, Mwanga-Amumpaire, Evans, Mwesigwa, Naome, Natukunda, Simpson, Nuwamanya, Miria, Nyangoma, Patrick, Orikiriza, Johnbosco, Tumwijukye, Esther, Turyashemererwa, Nyehangane, Dan, Mugisha, Ivan, Winnie, Biryeri, George, Naika, Robert, Ongwara O., Allen, Najjuko, Augustine, Kayiira, Samuel, Yairo, Immaculate, Tumwebaze, Goreth, Nalwoga, Paul, Nsiyaleta, Annet, Agaba, Martin, Mpimbaza M., Norbert, Akampurira, Agatha, Tugumisirize, Evans, Ariyo, Julius, Agaba, Yovita, Natukunda, Nelson, Musazi, Edmund, Musinguzi, Brown, Baluku Julius, Moorine, Sekadde, Stavia, Turyahabwe, Chishala, Chabala, Luis, Cuevas, Christophe, Delacourt, Steve, Graham, Malgorzata, Grzemska, Sabine, Verkuijl, Anneke, Hesseling, Elizabeth, Maleche-Obimbo, Mark, Nicol, Wobudeya, Eric, Nanfuka, Mastula, Ton Nu Nguyet, Minh Huyen, Taguebue, Jean-Voisin, Moh, Raoul, Breton, Guillaume, Khosa, Celso, Borand, Laurence, Mwanga-Amumpaire, Juliet, Mustapha, Ayeshatu, Nolna, Sylvie Kwedi, Komena, Eric, Mugisha, Jacob Ross, Natukunda, Naome, Dim, Bunnet, de Lauzanne, Agathe, Cumbe, Saniata, Balestre, Eric, Poublan, Julien, Lounnas, Manon, Ngu, Eden, Joshi, Basant, Norval, Pierre-Yves, Terquiem, Etienne Leroy, Turyahabwe, Stavia, Foray, Lynda, Sidibé, Souleymane, Manhiça, Ivan, Sekadde, Moorine, Detjen, Anne, Verkuijl, Sabine, Mao, Tan Eang, Orne-Gliemann, Joanna, Bonnet, Maryline, and Marcy, Olivier

Published
2024
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3. Effect of systematic tuberculosis detection on mortality in young children with severe pneumonia in countries with high incidence of tuberculosis: a stepped-wedge cluster-randomised trial

Author

Marcy, Olivier, Serre, Angeline, Badrichani, Anne, Razafimanantsoa, Manoa, Poublan, Julien, Vessière, Aurélia, Roucher, Clémentine, Occelli, Estelle, Beuscart, Aurélie, Charpin, Aurélie, Habiyambere, Gemma, Mesnier, Salomé, Balestre, Eric, Bhatta, Bandana, Maillard, Anne-Laure, Orne-Gliemann, Joanna, Baillet, Emmanuelle, Koskas, Nicolas, D'Elbée, Marc, Gabillard, Delphine, Font, Hélène, Huyen, Minh, Bonnet, Maryline, Lounnas, Manon, Espérou, Hélène, Couffin-Cadiergues, Sandrine, Kuppers, Alexis, Hamze, Benjamin, BORAND, Laurence, de LAUZANNE, Agathe, DIM, Bunnet, Keang, Chanthy, PRING, Long, YIN, Song, SARITH, Channimol, PHAN, Chanvirak, NHEUONG, Sovann, LY, Socheat, KAING, Sanary, SRENG, Vouchleang, LUN, Elen, SAY, Leakhena, SUOM, Sophea, FERHY, Romyka, SO, Dina, BORN, Sorunna, PAL, Sophea, NANG, Boraneath, MAO, Tan Eang, KIM, Ang, Srey, Viso, Kan, Piseth, Hout, leakhena, Ith, Samnang, Oum, Sophany, Sau, Sokunvadhana, Ho, Kim Heang, Kith, Daronic, Nuch, Nathara, Horm, Chhun Leang, Sophon, Cheameas, Roeungdeth, Bosba, MENG, Chhay, RITH, Ravin, PHY, Samnang, SOR, Chanchetra, SAO, Voleak, KHAT, Sophea, MAK, Bunthoeun, UY, Angkeaborin, KHAY, Sreyny, SOM, Kimsan, HACH, rongvirak, SOK, Hay, KUON, Sotheavy, HENG, Synatt, SENG, Amara, NIM, Sopheak, PAN, Reach, KIM, Srean, SREY LEAP, Keo, NET, Bormey, NOUN, Viccheka, LAY, Daven, MANY, Chhaing, Seng, Socheata, Ly, Vuthy, So, Saran, Oun, Sovutthik, CHEY, Sopheap, CHHEA, Rattany, BAONG, Lydeth, THOUNG, Vanna, KHEANG, Chanrithea, BY, Borady, Nguon, Vathanak, MEACH, Eksophea, Tek, Sopheak, Ngeav, Sina, Lun, Tetra, HEM, Deth, CHUT, Nayreang, SARIK, Setha, NANG, Hgekkoung, MEACH, Mengnean, SRENG, Sopal, SAR, Dara, KIN, Rathana, ROS, Phoran, DORN, Chenda, KAK, Chansy, Sambath, Srey Leak, Son, Leakhena, Bin, Linda, Pengong, Eangnay, Pol, Sokha, Khutsorn, Samnang, Seang, Sorsophea, Soun, Virak, Vong, Vuthy, Khoeung, Chandara, Um, Panha, Bou, Sokunthea, Song Pich, Sarin, Nim, Puthy, Khat, Sopheak, Ban Si, Nuon, Ream, Sovannodom, Ing, Sim, Chann, Phanith, Ngeth, Samrith, Sun, Marina, Chhoeung, Sokea, Sean, Soeun, Prak, Ratanak, Taguebue, Jean-Voisin, Kwedi Nolna, Sylvie, Amboua Schouame Onambele, Audrey, Hycenth, Numfor, Melingui, Bernard, Nkembe Medounmga, Angeline, Hougnang Tatmi, Luciole, Etemgoua, Nathalie, Kouesso, Vanessa, Bugin, Jean, Nzedjom, Celestine, Ngoya, Roger, Eyike, Jules, Loudjom, Elyse, Lonsti, Roger, Dang, Ladi, Bintar, Edward, Njayong, Chantal, Ngonsoa O, Cinthia, Ndzeukap, Isabelle, Dzoyem, Pascaline, Dzokou, Clémentine, Dindo, Berthe, Moh, Raoul, Komena, Eric Auguste, Aka Bony, Roger, Kouadio, Christian, Danho, Serge, Goli, Melissa, Folquet, Madeleine, Itchy, Max Valère, Sidibé, Abdel, Cissé, Lancina, Ouattara, Joseph, Konaté, Mamadou, Amon-Tanoh Dick, Flore, Cardena, Melissa, Adonis-Koffi, Laurence, Eugenie, Djabia, Kouamé, Ferdinand, Menan, Hervé, Inwoley, André, Ouassa, Timothée, Nguessan, Marcelle Sandrine, Khosa, Celso, Cumbe, Saniata, Manhiça, Emelva, Zitha, Alcina, Chiúle, Valter, Muxanga, Eva, Gune, Irene, Lima, Yara, Ribeiro, Jorge, Mavale, Sandra, Chilundo, Josina, Maxanguana, Felismina, Morais, Natália, Manhiça, Julieta, Give, Josefina, Atumane, Jafito, Lucas, Gelson, Thai, Arsénio, Chave, Adélio, Rego, Dalila, Guambe, Lúcia, Issa, Faiaz, Carneiro, Rosa, Pene, Neusa, Florindo, Natércia, Machel, Dália, Cumbane, Cecília, Mendes, Helena, Kitungwa, Mule, Muianga, Valdo, Tamele, Humberto, Sulude, Adelino, Mabota, Roda, Comandante, Herquéria, Massangaie, Abelardo, Wobudeya, Eric, Businge, Gerald Bright, Namulinda, Faith, Sserunjogi, Robert, Nassozi, Rashidah, Barungi, Charlotte, Aanyu, Hellen, Muwonge, Doreen, Kagoya, Eva, Aciparu, Serene, Chemutai, Sophia, Ntambi, Samuel, Wasswa, Amir, Nangozi, Juliet, Tagoola, Abner, Mbekeka, Prossy, Kenneth, Sajja, Lubega, John Paul, Nassali, Aidah, Tagobera, Jessica, Agwang, Christine, Kalembe, Florence, Ajambo, Annet, Aguti, Elizabeth, Kasibante, Samuel, Matende, Henry, Odongo, Israel Owen, Mwanga Amumpaire, Juliet, Natukunda, Naome, Ngabirano, Gertrude, Kakwenza, Paul, Nuwamanya, Simpson, Nyangoma, Miria, Nabbuto, Jane, Abok, Florence, Arinaitwe, Rinah, Birungi, Diana, Mwesigwa, Evans, Atwine, Daniel, Mbega, Hassan, Orikiriza, Patrick, Taremwa, Ivan, Turyashemererwa, Esther, Derrick, Hope, Nyehangane, Dan, Kaitano, Rodney, Logoose, Susan, Businge, Steven, Ntambi, Charles, Mugabi, Jerome, Mzee, John, Besigye, Julius, Kanzira, Saul, Turyatemba, Phionah, Twebaze, Florence, Chabala, Chishala, Mulenga, Veronica, Shankalala, Perfect, Hambulo, Chimuka, Kapotwe, Vincent, Ngambi, Marjory, Kasakwa, Kunda, Chirwa, Uzima, Kapula, Chifunda, Zulu, Susan, Nawakwi, Grace, Siasulingana, Teddy, Chilonga, Jessy, Chimbini, Maria, Chilanga, Mutinta, Nduna, Bwendo, Inambao, Muleya, Mwambazi, Mwate, Halende, Barbra, Mumba, Wyclef, Mankunshe, Endreen, Silavwe, Maureen, Chakopo, Moses, Moono, Roy, Mwanga-Amumpaire, Juliet, De Lauzanne, Agathe, Dim, Bunnet, Tiogouo Ngouana, Emeline, Folquet Amorrissani, Madeleine, Cisse, Lassina, Amon Tanoh Dick, Flore, Komena, Eric A, Businge, Gerald, Kim, Ang, Kheang, Chanrithea, Maleche-Obimbo, Elizabeth, Seddon, James A, Mao, Tan Eang, Graham, Stephen M, Delacourt, Christophe, and Borand, Laurence

Published
2023
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6. Immunogenicity and safety of fractional doses of yellow fever vaccines: a randomised, double-blind, non-inferiority trial

Author

Juan-Giner, Aitana, Kimathi, Derick, Grantz, Kyra H, Hamaluba, Mainga, Kazooba, Patrick, Njuguna, Patricia, Fall, Gamou, Dia, Moussa, Bob, Ndeye S, Monath, Thomas P, Barrett, Alan D, Hombach, Joachim, Mulogo, Edgar M, Ampeire, Immaculate, Karanja, Henry K, Nyehangane, Dan, Mwanga-Amumpaire, Juliet, Cummings, Derek A T, Bejon, Philip, Warimwe, George M, and Grais, Rebecca F

Published
2021
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7. Multiplex detection of meningitis pathogens by a vertical flow paper microarray and signal enhancement suitable for low-resource settings : Proof of concept

Author

Reu, Pedro, Gaudenzi, Giulia, Nanjebe, Deborah, Svedberg, Gustav, Nyehangane, Dan, Urrutia Iturritza, Miren, Mlotshwa, Phuthumani, Hadjineophytou, Chris, Karlsson, Jens, Gantelius, Jesper, Mwanga-Amumpaire, Juliet, Loh, Edmund, Svahn Andersson, Helene, Kumbakumba, Elias, Alfvén, Tobias, Boum II, Yap, Russom, Aman, Reu, Pedro, Gaudenzi, Giulia, Nanjebe, Deborah, Svedberg, Gustav, Nyehangane, Dan, Urrutia Iturritza, Miren, Mlotshwa, Phuthumani, Hadjineophytou, Chris, Karlsson, Jens, Gantelius, Jesper, Mwanga-Amumpaire, Juliet, Loh, Edmund, Svahn Andersson, Helene, Kumbakumba, Elias, Alfvén, Tobias, Boum II, Yap, and Russom, Aman

Abstract

Objectives: Meningitis is a medical emergency, and it is crucial to diagnose it accurately and promptly in order to manage patients effectively. It would, therefore, be essential to introduce and have fast, accurate, and user-friendly methods to determine the cause of these infections. This study aimed to demonstrate a potentially cost-effective new approach for detecting meningitis using a paper-based vertical flow microarray, which could be useful in settings with limited resources. Methods: We describe a multiplex paper microarray for detecting Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, and Salmonella spp. by the passive vertical flow of PCR-amplified clinical samples. A multibiotinylated amplicon was obtained as a product of PCR in the presence of both a biotinylated primer and biotin-11-dUTP. An enhancement step based on an enzyme-free gold enhancement protocol was also used to facilitate visual detection. Results: This study showed that the vertical flow microarray (previously evaluated for one pathogen) can discriminately detect the amplification results down to the 102 copies of DNA limit for four meningitis pathogens in a multiplexed set-up. The study further demonstrated the ability of this device and setup to detect three of the four pathogens from clinical biosamples. Discussion: This study demonstrated the capacity of a vertical flow microarray device to detect amplification products for four prevalent meningitis pathogens in a multiplex format. The vertical flow microarray demonstrated consistent visualization of the expected gene amplification results; however, indicating limitations in the pre- and amplification steps. This study highlights the potential of this multiplexing method for diagnosing meningitis and other syndromic diseases caused by various pathogens, especially in resource-limited areas., QC 20240926

Published
2024
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9. Diagnostic accuracy of Xpert MTB/RIF Ultra for tuberculous meningitis in HIV-infected adults: a prospective cohort study

Author

Williams, Darlisha A, Taseera, Kabanda, Nyehangane, Dan, Ivan, Mugisha, Orikiriza, Patrick, Rhein, Joshua, Hullsiek, Kathy Huppler, Musubire, Abdu, Pastick, Katelyn, Nabeta, Pamela, Mwesigye, James, Rajasingham, Radha, Bahr, Nathan C, Nuwagira, Edwin, Evans, Emily E, Cresswell, Fiona V, Bystrom, Philip V, Byamukama, Adolf, Bridge, Sarah C, Bangdiwala, Ananta S, Meya, David B, Denkinger, Claudia M, Muzoora, Conrad, and Boulware, David R

Published
2018
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13. It takes more than a machine: A pilot feasibility study of point-of-care HIV-1 viral load testing at a lower-level health center in rural western Uganda

Author

Boyce, Ross M., primary, Ndizeye, Ronnie, additional, Ngelese, Herbert, additional, Baguma, Emmanuel, additional, Shem, Bwambale, additional, Rubinstein, Rebecca J., additional, Rockwell, Emmanuel, additional, Lotspeich, Sarah C., additional, Shook-Sa, Bonnie E., additional, Ntaro, Moses, additional, Nyehangane, Dan, additional, Wohl, David A., additional, Siedner, Mark J., additional, and Mulogo, Edgar M., additional

Published
2023
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14. Mortality and Cause of Death in Children With Presumptive Disseminated Tuberculosis

Author

Bonnet, Maryline, primary, Nordholm, Anne-Christine, additional, Ssekyanzi, Bob, additional, Byamukama, Onesmus, additional, Orikiriza, Patrick, additional, Tusabe, Tobias, additional, Nyehangane, Dan, additional, Mugisha Taremwa, Ivan, additional, Turyashemererwa, Esther, additional, Wobudeya, Eric, additional, Mwanga-Amumpaire, Juliet, additional, Marais, Ben J, additional, and Nampijja, Dorah, additional

Published
2023
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15. Effect of systematic tuberculosis detection on mortality in young children with severe pneumonia in countries with high incidence of tuberculosis: a stepped-wedge cluster-randomised trial

Author

Marcy, Olivier, primary, Wobudeya, Eric, additional, Font, Hélène, additional, Vessière, Aurélia, additional, Chabala, Chishala, additional, Khosa, Celso, additional, Taguebue, Jean-Voisin, additional, Moh, Raoul, additional, Mwanga-Amumpaire, Juliet, additional, Lounnas, Manon, additional, Mulenga, Veronica, additional, Mavale, Sandra, additional, Chilundo, Josina, additional, Rego, Dalila, additional, Nduna, Bwendo, additional, Shankalala, Perfect, additional, Chirwa, Uzima, additional, De Lauzanne, Agathe, additional, Dim, Bunnet, additional, Tiogouo Ngouana, Emeline, additional, Folquet Amorrissani, Madeleine, additional, Cisse, Lassina, additional, Amon Tanoh Dick, Flore, additional, Komena, Eric A, additional, Kwedi Nolna, Sylvie, additional, Businge, Gerald, additional, Natukunda, Naome, additional, Cumbe, Saniata, additional, Mbekeka, Prossy, additional, Kim, Ang, additional, Kheang, Chanrithea, additional, Pol, Sokha, additional, Maleche-Obimbo, Elizabeth, additional, Seddon, James A, additional, Mao, Tan Eang, additional, Graham, Stephen M, additional, Delacourt, Christophe, additional, Borand, Laurence, additional, Bonnet, Maryline, additional, Marcy, Olivier, additional, Serre, Angeline, additional, Badrichani, Anne, additional, Razafimanantsoa, Manoa, additional, Poublan, Julien, additional, Roucher, Clémentine, additional, Occelli, Estelle, additional, Beuscart, Aurélie, additional, Charpin, Aurélie, additional, Habiyambere, Gemma, additional, Mesnier, Salomé, additional, Balestre, Eric, additional, Bhatta, Bandana, additional, Maillard, Anne-Laure, additional, Orne-Gliemann, Joanna, additional, Baillet, Emmanuelle, additional, Koskas, Nicolas, additional, D'Elbée, Marc, additional, Gabillard, Delphine, additional, Huyen, Minh, additional, Espérou, Hélène, additional, Couffin-Cadiergues, Sandrine, additional, Kuppers, Alexis, additional, Hamze, Benjamin, additional, BORAND, Laurence, additional, de LAUZANNE, Agathe, additional, DIM, Bunnet, additional, Keang, Chanthy, additional, PRING, Long, additional, YIN, Song, additional, SARITH, Channimol, additional, PHAN, Chanvirak, additional, NHEUONG, Sovann, additional, LY, Socheat, additional, KAING, Sanary, additional, SRENG, Vouchleang, additional, LUN, Elen, additional, SAY, Leakhena, additional, SUOM, Sophea, additional, FERHY, Romyka, additional, SO, Dina, additional, BORN, Sorunna, additional, PAL, Sophea, additional, NANG, Boraneath, additional, MAO, Tan Eang, additional, KIM, Ang, additional, Srey, Viso, additional, Kan, Piseth, additional, Hout, leakhena, additional, Ith, Samnang, additional, Oum, Sophany, additional, Sau, Sokunvadhana, additional, Ho, Kim Heang, additional, Kith, Daronic, additional, Nuch, Nathara, additional, Horm, Chhun Leang, additional, Sophon, Cheameas, additional, Roeungdeth, Bosba, additional, MENG, Chhay, additional, RITH, Ravin, additional, PHY, Samnang, additional, SOR, Chanchetra, additional, SAO, Voleak, additional, KHAT, Sophea, additional, MAK, Bunthoeun, additional, UY, Angkeaborin, additional, KHAY, Sreyny, additional, SOM, Kimsan, additional, HACH, rongvirak, additional, SOK, Hay, additional, KUON, Sotheavy, additional, HENG, Synatt, additional, SENG, Amara, additional, NIM, Sopheak, additional, PAN, Reach, additional, KIM, Srean, additional, SREY LEAP, Keo, additional, NET, Bormey, additional, NOUN, Viccheka, additional, LAY, Daven, additional, MANY, Chhaing, additional, Seng, Socheata, additional, Ly, Vuthy, additional, So, Saran, additional, Oun, Sovutthik, additional, CHEY, Sopheap, additional, CHHEA, Rattany, additional, BAONG, Lydeth, additional, THOUNG, Vanna, additional, KHEANG, Chanrithea, additional, BY, Borady, additional, Nguon, Vathanak, additional, MEACH, Eksophea, additional, Tek, Sopheak, additional, Ngeav, Sina, additional, Lun, Tetra, additional, HEM, Deth, additional, CHUT, Nayreang, additional, SARIK, Setha, additional, NANG, Hgekkoung, additional, MEACH, Mengnean, additional, SRENG, Sopal, additional, SAR, Dara, additional, KIN, Rathana, additional, ROS, Phoran, additional, DORN, Chenda, additional, KAK, Chansy, additional, Sambath, Srey Leak, additional, Son, Leakhena, additional, Bin, Linda, additional, Pengong, Eangnay, additional, Khutsorn, Samnang, additional, Seang, Sorsophea, additional, Soun, Virak, additional, Vong, Vuthy, additional, Khoeung, Chandara, additional, Um, Panha, additional, Bou, Sokunthea, additional, Song Pich, Sarin, additional, Nim, Puthy, additional, Khat, Sopheak, additional, Ban Si, Nuon, additional, Ream, Sovannodom, additional, Ing, Sim, additional, Chann, Phanith, additional, Ngeth, Samrith, additional, Sun, Marina, additional, Chhoeung, Sokea, additional, Sean, Soeun, additional, Prak, Ratanak, additional, Amboua Schouame Onambele, Audrey, additional, Hycenth, Numfor, additional, Melingui, Bernard, additional, Nkembe Medounmga, Angeline, additional, Hougnang Tatmi, Luciole, additional, Etemgoua, Nathalie, additional, Kouesso, Vanessa, additional, Bugin, Jean, additional, Nzedjom, Celestine, additional, Ngoya, Roger, additional, Eyike, Jules, additional, Loudjom, Elyse, additional, Lonsti, Roger, additional, Dang, Ladi, additional, Bintar, Edward, additional, Njayong, Chantal, additional, Ngonsoa O, Cinthia, additional, Ndzeukap, Isabelle, additional, Dzoyem, Pascaline, additional, Dzokou, Clémentine, additional, Dindo, Berthe, additional, Komena, Eric Auguste, additional, Aka Bony, Roger, additional, Kouadio, Christian, additional, Danho, Serge, additional, Goli, Melissa, additional, Folquet, Madeleine, additional, Itchy, Max Valère, additional, Sidibé, Abdel, additional, Cissé, Lancina, additional, Ouattara, Joseph, additional, Konaté, Mamadou, additional, Amon-Tanoh Dick, Flore, additional, Cardena, Melissa, additional, Adonis-Koffi, Laurence, additional, Eugenie, Djabia, additional, Kouamé, Ferdinand, additional, Menan, Hervé, additional, Inwoley, André, additional, Ouassa, Timothée, additional, Nguessan, Marcelle Sandrine, additional, Manhiça, Emelva, additional, Zitha, Alcina, additional, Chiúle, Valter, additional, Muxanga, Eva, additional, Gune, Irene, additional, Lima, Yara, additional, Ribeiro, Jorge, additional, Maxanguana, Felismina, additional, Morais, Natália, additional, Manhiça, Julieta, additional, Give, Josefina, additional, Atumane, Jafito, additional, Lucas, Gelson, additional, Thai, Arsénio, additional, Chave, Adélio, additional, Guambe, Lúcia, additional, Issa, Faiaz, additional, Carneiro, Rosa, additional, Pene, Neusa, additional, Florindo, Natércia, additional, Machel, Dália, additional, Cumbane, Cecília, additional, Mendes, Helena, additional, Kitungwa, Mule, additional, Muianga, Valdo, additional, Tamele, Humberto, additional, Sulude, Adelino, additional, Mabota, Roda, additional, Comandante, Herquéria, additional, Massangaie, Abelardo, additional, Businge, Gerald Bright, additional, Namulinda, Faith, additional, Sserunjogi, Robert, additional, Nassozi, Rashidah, additional, Barungi, Charlotte, additional, Aanyu, Hellen, additional, Muwonge, Doreen, additional, Kagoya, Eva, additional, Aciparu, Serene, additional, Chemutai, Sophia, additional, Ntambi, Samuel, additional, Wasswa, Amir, additional, Nangozi, Juliet, additional, Tagoola, Abner, additional, Kenneth, Sajja, additional, Lubega, John Paul, additional, Nassali, Aidah, additional, Tagobera, Jessica, additional, Agwang, Christine, additional, Kalembe, Florence, additional, Ajambo, Annet, additional, Aguti, Elizabeth, additional, Kasibante, Samuel, additional, Matende, Henry, additional, Odongo, Israel Owen, additional, Mwanga Amumpaire, Juliet, additional, Ngabirano, Gertrude, additional, Kakwenza, Paul, additional, Nuwamanya, Simpson, additional, Nyangoma, Miria, additional, Nabbuto, Jane, additional, Abok, Florence, additional, Arinaitwe, Rinah, additional, Birungi, Diana, additional, Mwesigwa, Evans, additional, Atwine, Daniel, additional, Mbega, Hassan, additional, Orikiriza, Patrick, additional, Taremwa, Ivan, additional, Turyashemererwa, Esther, additional, Derrick, Hope, additional, Nyehangane, Dan, additional, Kaitano, Rodney, additional, Logoose, Susan, additional, Businge, Steven, additional, Ntambi, Charles, additional, Mugabi, Jerome, additional, Mzee, John, additional, Besigye, Julius, additional, Kanzira, Saul, additional, Turyatemba, Phionah, additional, Twebaze, Florence, additional, Hambulo, Chimuka, additional, Kapotwe, Vincent, additional, Ngambi, Marjory, additional, Kasakwa, Kunda, additional, Kapula, Chifunda, additional, Zulu, Susan, additional, Nawakwi, Grace, additional, Siasulingana, Teddy, additional, Chilonga, Jessy, additional, Chimbini, Maria, additional, Chilanga, Mutinta, additional, Inambao, Muleya, additional, Mwambazi, Mwate, additional, Halende, Barbra, additional, Mumba, Wyclef, additional, Mankunshe, Endreen, additional, Silavwe, Maureen, additional, Chakopo, Moses, additional, and Moono, Roy, additional

Published
2022
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16. Additional file 1 of Permethrin-treated baby wraps for the prevention of malaria: results of a randomized controlled pilot study in rural Uganda

Author

Boyce, Ross M., Muhindo, Enid, Baguma, Emmanuel, Muhindo, Rabbison, Shem, Bwambale, Fran��ois, Ruthly, Hawke, Sam, Shook-Sa, Bonnie E., Ntaro, Moses, Nalusaji, Aisha, Nyehangane, Dan, Reyes, Raquel, Juliano, Jonathan J., Siedner, Mark J., Staedke, Sarah G., and Mulogo, Edgar M.

Abstract

Additional file 1. Baseline Survey.

Published
2022
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17. Additional file 1 of Evaluation of HRP2 and pLDH-based rapid diagnostic tests for malaria and prevalence of pfhrp2/3 deletions in Aweil, South Sudan

Author

Lynch, Emily, Jensen, Tomas O., Assao, Bachir, Chihana, Menard, Turuho, Thadeous, Nyehangane, Dan, Manyok, John B., Pasquale, Harriet, Khim, Nimol, Witkowski, Benoit, and Coldiron, Matthew E.

Abstract

Additional file 1. Primer sequences and PCR conditions for PCR testing of study samples at Institute Pasteur Cambodia.

Published
2022
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19. Additional file 1 of Surveillance of Plasmodium falciparum pfcrt haplotypes in southwestern Uganda by high‐resolution melt analysis

Author

Kassaza, Kennedy, Long, Anna C., McDaniels, Jennifer M., Mharlove Andre, Wasswa Fredrickson, Nyehangane, Dan, Orikiriza, Patrick, Operario, Darwin J., Bazira, Joel, Mwanga-Amumpaire, Juliet A., Moore, Christopher C., Guler, Jennifer L., and Yap Boum

Subjects
parasitic diseases
Abstract

Additional file 1: Table S1. Summary of HighResolution Melt analysis on clinical samples. Figure S1. Targeted sequencingconfirms pfcrt haplotypes in control Plasmodium genomes. Figure S2. Change in normalization boundaries does notimpact automatic haplotype calling. Figure S3. Quantitative PCR Ct values are inverselyproportional to HRM confidence percentages.

Published
2021
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20. Population Pharmacokinetics and Significant Under-Dosing of Anti-Tuberculosis Medications in People with HIV and Critical Illness

Author

Rao, Prakruti S., primary, Moore, Christopher C., additional, Mbonde, Amir A., additional, Nuwagira, Edwin, additional, Orikiriza, Patrick, additional, Nyehangane, Dan, additional, Al-Shaer, Mohammad H., additional, Peloquin, Charles A., additional, Gratz, Jean, additional, Pholwat, Suporn, additional, Arinaitwe, Rinah, additional, Boum, Yap, additional, Mwanga-Amumpaire, Juliet, additional, Houpt, Eric R., additional, Kagan, Leonid, additional, Heysell, Scott K., additional, and Muzoora, Conrad, additional

Published
2021
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21. Tuberculosis diagnostic accuracy of stool Xpert MTB/RIF and urine AlereLAM in vulnerable children

Author

Orikiriza, Patrick, primary, Smith, Julianna, additional, Ssekyanzi, Bob, additional, Nyehangane, Dan, additional, Mugisha Taremwa, Ivan, additional, Turyashemererwa, Esther, additional, Byamukama, Onesmas, additional, Tusabe, Tobias, additional, Ardizzoni, Elisa, additional, Marais, Ben J, additional, Wobudeya, Eric, additional, Kemigisha, Elizabeth, additional, Mwanga-Amumpaire, Juliet, additional, Nampijja, Dora, additional, and Bonnet, Maryline, additional

Published
2021
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23. Dengue in western Uganda: A prospective cohort of children presenting with undifferentiated febrile illness

Author

Boyce, Ross M., primary, Collins, Matthew, additional, Muhindo, Rabbison, additional, Nakakande, Regina, additional, Ciccone, Emily J., additional, Grounds, Samantha, additional, Espinoza, Daniel, additional, Zhu, Yerun, additional, Matte, Michael, additional, Ntaro, Moses, additional, Nyehangane, Dan, additional, Juliano, Jonathan J., additional, and Mulogo, Edgar M., additional

Published
2020
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24. Immunogenicity and safety of fractional doses of yellow fever vaccines: a randomized, double-blind, non-inferiority trial

Author

Juan-Giner, Aitana, primary, Kimathi, Derick, additional, Grantz, Kyra H., additional, Hamaluba, Mainga M., additional, Kazooba, Patrick, additional, Njuguna, Patricia, additional, Fall, Gamou, additional, Dia, Moussa, additional, Bob, Ndeye S., additional, Monath, Thomas P., additional, Barrett, Alan D., additional, Hombach, Joachim, additional, Mulogo, Edgar M., additional, Ampeire, Immaculate, additional, Karanja, Henry K., additional, Nyehangane, Dan, additional, Mwanga-Amumpaire, Juliet, additional, Cummings, Derek A.T., additional, Bejon, Philip, additional, Warimwe, George M., additional, and Grais, Rebecca F., additional

Published
2020
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25. Point-of-Care Approaches for Meningitis Diagnosis in a Low-Resource Setting (Southwestern Uganda): Observational Cohort Study Protocol of the “PI-POC” Trial (Preprint)

Author

Gaudenzi, Giulia, primary, Kumbakumba, Elias, additional, Rasti, Reza, additional, Nanjebe, Deborah, additional, Réu, Pedro, additional, Nyehangane, Dan, additional, Mårtensson, Andreas, additional, Nassejje, Milly, additional, Karlsson, Jens, additional, Mzee, John, additional, Nilsson, Peter, additional, Businge, Stephen, additional, Loh, Edmund, additional, Boum II, Yap, additional, Andersson-Svahn, Helene, additional, Gantelius, Jesper, additional, Mwanga-Amumpaire, Juliet, additional, and Alfvén, Tobias, additional

Published
2020
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26. Artemether-lumefantrine to treat malaria in pregnancy is associated with reduced placental haemozoin deposition compared to quinine in a randomized controlled trial

Author

Muehlenbachs Atis, Nabasumba Carolyn, McGready Rose, Turyakira Eleanor, Tumwebaze Benon, Dhorda Mehul, Nyehangane Dan, Nalusaji Aisha, Nosten Franois, Guerin Philippe J, and Piola Patrice

Subjects
Malaria in pregnancy, Placental malaria, Artemisinin-based combination therapy, Quinine, Artemether-lumefantrine, Falciparum, Pathology, Histology, Randomized controlled trial, Haemozoin, Arctic medicine. Tropical medicine, RC955-962, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Data on efficacy of artemisinin-based combination therapy (ACT) to treat Plasmodium falciparum during pregnancy in sub-Saharan Africa is scarce. A recent open label, randomized controlled trial in Mbarara, Uganda demonstrated that artemether-lumefantrine (AL) is not inferior to quinine to treat uncomplicated malaria in pregnancy. Haemozoin can persist in the placenta following clearance of parasites, however there is no data whether ACT can influence the amount of haemozoin or the dynamics of haemozoin clearance. Methods Women attending antenatal clinics with weekly screening and positive blood smears by microscopy were eligible to participate in the trial and were followed to delivery. Placental haemozoin deposition and inflammation were assessed by histology. To determine whether AL was associated with increased haemozoin clearance, population haemozoin clearance curves were calculated based on the longitudinal data. Results Of 152 women enrolled in each arm, there were 97 and 98 placental biopsies obtained in the AL and quinine arms, respectively. AL was associated with decreased rates of moderate to high grade haemozoin deposition (13.3% versus 25.8%), which remained significant after correcting for gravidity, time of infection, re-infection, and parasitaemia. The amount of haemozoin proportionately decreased with the duration of time between treatment and delivery and this decline was greater in the AL arm. Haemozoin was not detected in one third of biopsies and the prevalence of inflammation was low, reflecting the efficacy of antenatal care with early detection and prompt treatment of malaria. Conclusions Placental haemozoin deposition was decreased in the AL arm demonstrating a relationship between pharmacological properties of drug to treat antenatal malaria and placental pathology at delivery. Histology may be considered an informative outcome for clinical trials to evaluate malaria control in pregnancy. Trial registration REGISTRY: http://clinicaltrials.gov/ct2/show/NCT00495508

Published
2012
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28. Carriage prevalence and serotype distribution of Streptococcus pneumoniae prior to 10-valent pneumococcal vaccine introduction: A population-based cross-sectional study in South Western Uganda, 2014

Author

Nackers, Fabienne, Cohuet, Sandra, le Polain de Waroux, Olivier, Langendorf, Céline, Nyehangane, Dan, Ndazima, Donny, Nanjebe, Deborah, Karani, Angela, Tumwesigye, Elioda, Mwanga-Amumpaire, Juliet, Scott, J Anthony G, and Grais, Rebecca F

Abstract

BACKGROUND: Information on Streptococcus pneumoniae nasopharyngeal (NP) carriage before the pneumococcal conjugate vaccine (PCV) introduction is essential to monitor impact. The 10-valent PCV (PCV10) was officially introduced throughout Ugandan national childhood immunization programs in 2013 and rolled-out countrywide during 2014. We aimed to measure the age-specific Streptococcus pneumoniae carriage and serotype distribution across all population age groups in the pre-PCV10 era in South Western Uganda. METHODS: We conducted a two-stage cluster, age-stratified, cross-sectional community-based study in Sheema North sub-district between January and March 2014. One NP swab was collected and analyzed for each participant in accordance with World Health Organization guidelines. RESULTS: NP carriage of any pneumococcal serotype was higher among children

Published
2017

29. Effect of high-dose rifampicin on efavirenz pharmacokinetics: drug-drug interaction randomized trial.

Author

Atwine, Daniel, Baudin, Elisabeth, Gelé, Thibaut, Muyindike, Winnie, Mworozi, Kenneth, Kyohairwe, Racheal, Kananura, Keneth, Orikiriza, Patrick, Nyehangane, Dan, Nanjebe, Deborah K.T., Furlan, Valérie, Verstuyft, Céline, Barrail-Tran, Aurélie, Taburet, Anne-Marie, Bonnet, Maryline, group, ANRS 12292 Rifavirenz study, K T Nanjebe, Deborah, and ANRS 12292 Rifavirenz study group

Subjects
HIV infections, ANTI-HIV agents, RESEARCH, HETEROCYCLIC compounds, RESEARCH methodology, MEDICAL cooperation, EVALUATION research, HYDROCARBONS, COMPARATIVE studies, DRUGS, DRUG interactions, RIFAMPIN
Abstract

Background: High-dose rifampicin is considered to shorten anti-TB treatment duration but its effect on antiretroviral metabolism is unknown.Objectives: To assess the effect of doubling the rifampicin dose (to 20 mg/kg/day, R20) on efavirenz pharmacokinetics (PK) in HIV/TB coinfected patients.Methods: Open-label Phase 2 drug-drug interaction randomized trial. Pulmonary TB, ART-naive adults were randomized to R20 and either efavirenz 600 mg (EFV600) or 800 mg (EFV800), or rifampicin 10 mg/kg/day (R10) and EFV600 with a 1:1:1 ratio. Patients were first started on TB treatment and 2-4 weeks later started on ART. They were switched to R10 and EFV600 after 8 weeks. Full PK sampling was done 4 weeks (on rifampicin) and 24 weeks (off rifampicin) after ART initiation. Transaminases, plasma HIV-1 RNA and sputum cultures were monitored. The efavirenz geometric mean ratio (GMR) of AUC at 4 and 24 weeks after ART initiation within the same patient was calculated in each arm and its 90% CI was compared with a preset range (0.70-1.43).Results: Of 98 enrolled patients (32 in the R20EFV600 arm, 33 in the R20EFV800 arm and 33 in the R10EFV600 arm), 87 had full PK sampling. For the R20EFV600, R20EFV800 and R10EFV600 arms, GMRs of efavirenz AUC were 0.87 (90% CI: 0.75-1.00), 1.12 (90% CI: 0.96-1.30) and 0.96 (90% CI: 0.84-1.10). Twelve weeks after ART initiation, 78.6%, 77.4% and 72.4% of patients had HIV-1 RNA below 100 copies/mL and 85.7%, 86.7% and 80.0% had Week 8 culture conversion, respectively. Two patients per arm experienced a severe increase in transaminases.Conclusions: Doubling the rifampicin dose had a small effect on efavirenz concentrations and was well tolerated. [ABSTRACT FROM AUTHOR]

Published
2020
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30. Antimicrobial treatment practices among Ugandan children with suspicion of central nervous system infection

Author

Kemigisha, Elizabeth, primary, Nanjebe, Deborah, additional, Boum, Yap, additional, Langendorf, Céline, additional, Aberrane, Said, additional, Nyehangane, Dan, additional, Nackers, Fabienne, additional, Mueller, Yolanda, additional, Charrel, Rémi, additional, Murphy, Richard A., additional, Page, Anne-Laure, additional, and Mwanga-Amumpaire, Juliet, additional

Published
2018
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31. Risk Factors for Vaginal Colonization and Relationship between Bacterial Vaginal Colonization and In-Hospital Outcomes in Women with Obstructed Labor in a Ugandan Regional Referral Hospital

Author

Ngonzi, Joseph, primary, Bebell, Lisa M., additional, Bazira, Joel, additional, Fajardo, Yarine, additional, Nyehangane, Dan, additional, Boum, Yap, additional, Nanjebe, Deborah, additional, Boatin, Adeline, additional, Kabakyenga, Jerome, additional, Jacquemyn, Yves, additional, Van Geertruyden, Jean-Pierre, additional, and Riley, Laura E., additional

Published
2018
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32. Detection of Plasmodium Species by High-Resolution Melt Analysis of DNA from Blood Smears Acquired in Southwestern Uganda

Author

Kassaza, Kennedy, primary, Operario, Darwin J., additional, Nyehangane, Dan, additional, Coffey, K. C., additional, Namugosa, Mary, additional, Turkheimer, Lena, additional, Ojuka, Patrick, additional, Orikiriza, Patrick, additional, Mwanga-Amumpaire, Juliet, additional, Byarugaba, Frederick, additional, Bazira, Joel, additional, Guler, Jennifer L., additional, Moore, Christopher C., additional, and Boum, Yap, additional

Published
2018
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33. Diagnostic accuracy of Xpert MTB/RIF Ultra for tuberculous meningitis in HIV-infected adults: a prospective cohort study

Author

Bahr, Nathan C, primary, Nuwagira, Edwin, additional, Evans, Emily E, additional, Cresswell, Fiona V, additional, Bystrom, Philip V, additional, Byamukama, Adolf, additional, Bridge, Sarah C, additional, Bangdiwala, Ananta S, additional, Meya, David B, additional, Denkinger, Claudia M, additional, Muzoora, Conrad, additional, Boulware, David R, additional, Williams, Darlisha A, additional, Taseera, Kabanda, additional, Nyehangane, Dan, additional, Ivan, Mugisha, additional, Orikiriza, Patrick, additional, Rhein, Joshua, additional, Hullsiek, Kathy Huppler, additional, Musubire, Abdu, additional, Pastick, Katelyn, additional, Nabeta, Pamela, additional, Mwesigye, James, additional, and Rajasingham, Radha, additional

Published
2018
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34. MOESM2 of Performance and time to become negative after treatment of three malaria rapid diagnostic tests in low and high malaria transmission settings

Author

Grandesso, Francesco, Nabasumba, Carolyn, Nyehangane, Dan, Anne-Laure Page, Bastard, Mathieu, Smet, Martin De, Yap Boum, and Jean-FrançOis Etard

Subjects
parasitic diseases
Abstract

Additional file 2. Additional analysis of the time to become negative for the three malaria RDTs where patients experiencing recurrent parasitaemia were censored at the day when recurrent parasitaemia occurred.

Published
2016
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35. Aetiology and Outcomes of Suspected Infections of the Central Nervous System in Children in Mbarara, Uganda

Author

Page, Anne-Laure, primary, Boum II, Yap, additional, Kemigisha, Elizabeth, additional, Salez, Nicolas, additional, Nanjebe, Deborah, additional, Langendorf, Céline, additional, Aberrane, Said, additional, Nyehangane, Dan, additional, Nackers, Fabienne, additional, Baron, Emmanuel, additional, Charrel, Rémi, additional, and Mwanga-Amumpaire, Juliet, additional

Published
2017
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37. Improving the Specificity of Plasmodium falciparum Malaria Diagnosis in High-Transmission Settings with a Two-Step Rapid Diagnostic Test and Microscopy Algorithm

Author

Murungi, Moses, primary, Fulton, Travis, additional, Reyes, Raquel, additional, Matte, Michael, additional, Ntaro, Moses, additional, Mulogo, Edgar, additional, Nyehangane, Dan, additional, Juliano, Jonathan J., additional, Siedner, Mark J., additional, Boum, Yap, additional, and Boyce, Ross M., additional

Published
2017
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38. Antimicrobial-resistant infections among postpartum women at a Ugandan referral hospital

Author

Bebell, Lisa M., primary, Ngonzi, Joseph, additional, Bazira, Joel, additional, Fajardo, Yarine, additional, Boatin, Adeline A., additional, Siedner, Mark J., additional, Bassett, Ingrid V., additional, Nyehangane, Dan, additional, Nanjebe, Deborah, additional, Jacquemyn, Yves, additional, van Geertruyden, Jean-Pierre, additional, Mwanga-Amumpaire, Juliet, additional, Bangsberg, David R., additional, Riley, Laura E., additional, and Boum, Yap, additional

Published
2017
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41. Diagnostic Accuracy of the Small Membrane Filtration Method for Diagnosis of Pulmonary Tuberculosis in a High-HIV-Prevalence Setting

Author

Boum, Yap, primary, Kim, Soyeon, additional, Orikiriza, Patrick, additional, Acuña-Villaorduña, Carlos, additional, Vinhas, Solange, additional, Bonnet, Maryline, additional, Nyehangane, Dan, additional, Mwanga-Amumpaire, Juliet, additional, Fennelly, Kevin P., additional, and Jones-López, Edward C., additional

Published
2016
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43. Inhaled Nitric Oxide as an Adjunctive Treatment for Cerebral Malaria in Children: A Phase II Randomized Open-Label Clinical Trial

Author

Mwanga-Amumpaire, Juliet, primary, Carroll, Ryan W., additional, Baudin, Elisabeth, additional, Kemigisha, Elisabeth, additional, Nampijja, Dorah, additional, Mworozi, Kenneth, additional, Santorino, Data, additional, Nyehangane, Dan, additional, Nathan, Daniel I., additional, De Beaudrap, Pierre, additional, Etard, Jean-François, additional, Feelisch, Martin, additional, Fernandez, Bernadette O., additional, Berssenbrugge, Annie, additional, Bangsberg, David, additional, Bloch, Kenneth D., additional, Boum, Yap, additional, and Zapol, Warren M., additional

Published
2015
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46. Performance of a Histidine-Rich Protein 2 Rapid Diagnostic Test, Paracheck Pf®, for Detection of Malaria Infections in Ugandan Pregnant Women

Author

Dhorda, Mehul, primary, Piola, Patrice, additional, Ashley, Elizabeth, additional, Turyakira, Eleanor, additional, Nabasumba, Carolyn, additional, Snounou, Georges, additional, Tumwebaze, Benon, additional, Nalusaji, Aisha, additional, Guerin, Philippe J., additional, McGready, Rose, additional, and Nyehangane, Dan, additional

Published
2012
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48. Improving the Specificity of Plasmodium falciparumMalaria Diagnosis in High-Transmission Settings with a Two-Step Rapid Diagnostic Test and Microscopy Algorithm

Author

Murungi, Moses, Fulton, Travis, Reyes, Raquel, Matte, Michael, Ntaro, Moses, Mulogo, Edgar, Nyehangane, Dan, Juliano, Jonathan J., Siedner, Mark J., Boum, Yap, and Boyce, Ross M.

Abstract

ABSTRACTPoor specificity may negatively impact rapid diagnostic test (RDT)-based diagnostic strategies for malaria. We performed real-time PCR on a subset of subjects who had undergone diagnostic testing with a multiple-antigen (histidine-rich protein 2 and pan-lactate dehydrogenase pLDH [HRP2/pLDH]) RDT and microscopy. We determined the sensitivity and specificity of the RDT in comparison to results of PCR for the detection of Plasmodium falciparummalaria. We developed and evaluated a two-step algorithm utilizing the multiple-antigen RDT to screen patients, followed by confirmatory microscopy for those individuals with HRP2-positive (HRP2+)/pLDH-negative (pLDH-) results. In total, dried blood spots (DBS) were collected from 276 individuals. There were 124 (44.9%) individuals with an HRP2+/pLDH+result, 94 (34.1%) with an HRP2+/pLDH-result, and 58 (21%) with a negative RDT result. The sensitivity and specificity of the RDT compared to results with real-time PCR were 99.4% (95% confidence interval [CI], 95.9 to 100.0%) and 46.7% (95% CI, 37.7 to 55.9%), respectively. Of the 94 HRP2+/pLDH-results, only 32 (34.0%) and 35 (37.2%) were positive by microscopy and PCR, respectively. The sensitivity and specificity of the two-step algorithm compared to results with real-time PCR were 95.5% (95% CI, 90.5 to 98.0%) and 91.0% (95% CI, 84.1 to 95.2), respectively. HRP2 antigen bands demonstrated poor specificity for the diagnosis of malaria compared to that of real-time PCR in a high-transmission setting. The most likely explanation for this finding is the persistence of HRP2 antigenemia following treatment of an acute infection. The two-step diagnostic algorithm utilizing microscopy as a confirmatory test for indeterminate HRP2+/pLDH-results showed significantly improved specificity with little loss of sensitivity in a high-transmission setting.

Published
2017
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50. Detection of PlasmodiumSpecies by High-Resolution Melt Analysis of DNA from Blood Smears Acquired in Southwestern Uganda

Author

Kassaza, Kennedy, Operario, Darwin J., Nyehangane, Dan, Coffey, K. C., Namugosa, Mary, Turkheimer, Lena, Ojuka, Patrick, Orikiriza, Patrick, Mwanga-Amumpaire, Juliet, Byarugaba, Frederick, Bazira, Joel, Guler, Jennifer L., Moore, Christopher C., and Boum, Yap

Abstract

ABSTRACTMicroscopic diagnosis of malaria using Giemsa-stained blood smears is the standard of care in resource-limited settings. These smears represent a potential source of DNA for PCR testing to confirm Plasmodiuminfections or for epidemiological studies of archived samples. Therefore, we assessed the use of DNA extracts from stained blood smears for the detection of Plasmodiumspecies using real-time PCR. We extracted DNA from archived blood smears and corresponding red blood cell pellets collected from asymptomatic children in southwestern Uganda in 2010. We then performed real-time PCR followed by high-resolution melting (HRM) to identify Plasmodiumspecies, and we compared our results to those of microscopy. We analyzed a total of 367 blood smears and corresponding red blood cell pellets, including 185 smears (50.4%) that were positive by microscopy. Compared to microscopy, PCR-HRM analysis of smear DNA had a sensitivity of 93.0% (95% confidence interval [CI], 88.2 to 96.2%) and a specificity of 96.7% (95% CI, 93.0 to 98.8%), and PCR-HRM analysis of pellet DNA had a sensitivity of 100.0% (95% CI, 98.0 to 100.0%) and a specificity of 94.0% (95% CI, 89.4 to 96.9%). Identification of positive PCR-HRM results to the species level revealed Plasmodium falciparum(92.0%), Plasmodium ovale(5.6%), and Plasmodium malariae(2.4%). PCR-HRM analysis of DNA extracts from Giemsa-stained thick blood smears or corresponding blood pellets had high sensitivity and specificity for malaria diagnosis, compared to microscopy. Therefore, blood smears can provide an adequate source of DNA for confirmation of Plasmodiumspecies infections and can be used for retrospective genetic studies.

Published
2017
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