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4 results on '"Nyakilinga, Denis"'

1. Molecular characterization of porcine circovirus-2 and -3 in pigs in Tanzania

Author

CHANG’A, Jelly S., primary, BITANYI, Stella S., additional, KAMIGWE, Aloyce, additional, MAGIDANGA, Bishop, additional, GUO, Shukuru, additional, MAKOROMA, Paulina, additional, FRANCIS, Gundelinda, additional, JUMBE, Jumanne, additional, JEREMIAH, Mashaka, additional, NYAKILINGA, Denis, additional, MWASHA, Mercy, additional, MSANGI, Kimweri, additional, CATTOLI, Giovanni, additional, FRANZO, Giovanni, additional, MOLINI, Umberto, additional, and DUNDON, William G, additional

Published
2023
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2. Molecular characterization of porcine circovirus-2 and -3 in pigs in Tanzania.

Author

CHANG'A, Jelly S., BITANYI, Stella S., KAMIGWE, Aloyce, MAGIDANGA, Bishop, GUO, Shukuru, MAKOROMA, Paulina, FRANCIS, Gundelinda, JUMBE, Jumanne, JEREMIAH, Mashaka, NYAKILINGA, Denis, MWASHA, Mercy, MSANGI, Kimweri, CATTOLI, Giovanni, FRANZO, Giovanni, MOLINI, Umberto, and DUNDON, William G.

Subjects
DISEASE management, SWINE, SEQUENCE analysis, PRODUCTION increases
Abstract

Like in many other African countries, pig production is increasing in Tanzania. To support farmers and stakeholders, it is important to increase our understanding of porcine pathogens present in the country. Currently, little is known about the circulation of porcine circovirus-2 (PCV-2) and porcine circovirus-3 (PCV-3). For this reason, samples from 124 pigs collected throughout the country between 2018 to 2022 were screened by PCR for the presence of PCV-2 and PCV-3. Sequencing and phylogenetic analysis of positive amplicons identified two PCV-2 genotypes (a and d). Limited genetic heterogenicity was observed among the PCV-3. This study provides important data on pathogens present in pigs in Tanzania and should be of use veterinary authorities involved in porcine disease management. [ABSTRACT FROM AUTHOR]

Published
2023
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3. External quality assessment of Rift Valley fever diagnosis in countries at risk of the disease: African, Indian Ocean and Middle-East regions

Author

Pédarrieu, Aurélie, El Mellouli, Fatiha, Khallouki, Hanane, Zro, Khalil, Sebbar, Ghizlane, Sghaier, Soufien, Madani, Hafsa, Bouayed, Nadera, Lo, Modou Moustapha, Diop, Mariame, Ould El Mamy, Ahmed Bezeid, Barry, Yahya, Dakouo, Marthin, Traoré, Abdallah, Gagara, Haladou, Souley, Maman Moutari, Achá, Sara, Mapaco, Lourenço, Chang'a, Jelly, Nyakilinga, Denis, Lubisi, Baratang A., Tshabalala, Thabisile, Filippone, Claudia, Héraud, Jean Michel, Chamassy, Sitti Bahyat, Achiraffi, Abdou, Keck, Nicolas, Grard, Gilda, Mohammed, Kareem Abdelfattah Abdelwahab, Alrizqi, Abdulwahed Mohammed, Cetre-Sossah, Catherine, Pédarrieu, Aurélie, El Mellouli, Fatiha, Khallouki, Hanane, Zro, Khalil, Sebbar, Ghizlane, Sghaier, Soufien, Madani, Hafsa, Bouayed, Nadera, Lo, Modou Moustapha, Diop, Mariame, Ould El Mamy, Ahmed Bezeid, Barry, Yahya, Dakouo, Marthin, Traoré, Abdallah, Gagara, Haladou, Souley, Maman Moutari, Achá, Sara, Mapaco, Lourenço, Chang'a, Jelly, Nyakilinga, Denis, Lubisi, Baratang A., Tshabalala, Thabisile, Filippone, Claudia, Héraud, Jean Michel, Chamassy, Sitti Bahyat, Achiraffi, Abdou, Keck, Nicolas, Grard, Gilda, Mohammed, Kareem Abdelfattah Abdelwahab, Alrizqi, Abdulwahed Mohammed, and Cetre-Sossah, Catherine

Abstract

Middle East, North Africa, South Africa, and the Indian Ocean participated in the initiative. Two commercial kits and two in-house serological assays for the detection of RVFV specific IgG antibodies were tested. Sixteen of the 18 participating laboratories (88.9%) used com- mercial kits, the analytical performance of test sensitivity and specificity based on the sero- neutralisation test considered as the reference was 100%. The results obtained by the laboratories which used the in-house assay were correct in only one of the two criteria (either sensitivity or specificity). In conclusion, most of the laboratories performed well in detecting RVFV specific IgG antibodies and can therefore be considered to be prepared. Three laboratories in three countries need to improve their detection capacities. Our study demonstrates the importance of conducting regular proficiency tests to evaluate the level of preparedness of countries and of building a network of competent laboratories in terms of laboratory diagnosis to better face future emerging diseases in emergency conditions.

Published
2021

4. External quality assessment of Rift Valley fever diagnosis in countries at risk of the disease: African, Indian Ocean and Middle-East regions

Author

Pedarrieu, Aurélie, primary, El Mellouli, Fatiha, additional, Khallouki, Hanane, additional, Zro, Khalil, additional, Sebbar, Ghizlane, additional, Sghaier, Soufien, additional, Madani, Hafsa, additional, Bouayed, Nadera, additional, Lo, Modou Moustapha, additional, Diop, Mariame, additional, Ould El Mamy, Ahmed Bezeid, additional, Barry, Yahya, additional, Dakouo, Marthin, additional, Traore, Abdallah, additional, Gagara, Haladou, additional, Souley, Maman Moutari, additional, Acha, Sara, additional, Mapaco, Laurenco, additional, Chang’a, Jelly, additional, Nyakilinga, Denis, additional, Lubisi, Baratang A., additional, Tshabalala, Thabisile, additional, Filippone, Claudia, additional, Heraud, Jean Michel, additional, Chamassy, Sitty-Bahyat, additional, Achiraffi, Abdou, additional, Keck, Nicolas, additional, Grard, Gilda, additional, Mohammed, Kareem Abdelfattah Abdelwahab, additional, Alrizqi, Abdulwahed Mohammed, additional, and Cetre-Sossah, Catherine, additional

Published
2021
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