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7. Cytokine profile of autologous platelet-derived eye drops in patients with ocular chronic graft-versus-host disease

Author

Valentini, Caterina Giovanna, Nuzzolo, Eugenia Rosa, Orlando, N., Metafuni, Elisabetta, Bianchi, M., Chiusolo, Patrizia, Zini Tanzi, Gina, Teofili, Luciana, Chiusolo, Patrizia (ORCID:0000-0002-1355-1587), Zini, Gina (ORCID:0000-0002-8208-066X), Teofili, Luciana (ORCID:0000-0002-7214-1561), Valentini, Caterina Giovanna, Nuzzolo, Eugenia Rosa, Orlando, N., Metafuni, Elisabetta, Bianchi, M., Chiusolo, Patrizia, Zini Tanzi, Gina, Teofili, Luciana, Chiusolo, Patrizia (ORCID:0000-0002-1355-1587), Zini, Gina (ORCID:0000-0002-8208-066X), and Teofili, Luciana (ORCID:0000-0002-7214-1561)

Abstract

Ocular chronic GVHD is efficaciously treated with autologous platelet-derived eye drops. We investigated the cytokine content of eye drops produced using a non-gelified lysate obtained from autologous platelet-rich plasma in six patients with ocular GVHD. In both the responding (n = 4) and the resistant (n = 2) patients, the eye drops were significantly enriched with various growth factors, in amounts proportional with the platelet counts. In contrast, chemokine ligand and interleukin levels were similar to those of plasma. The non-responding patients showed the highest levels of chemokine (C-X-C motif) ligand (CXCL)10. These findings provide possible explanations for beneficial or detrimental effects of eye drops.

Published
2016

8. An abnormal secretion of soluble mediators contributes to the hematopoietic-niche dysfunction in low-risk myelodysplastic syndrome

Author

Martini, Maurizio, Capodimonti, Sara, Iachininoto, Maria Grazia, Cocomazzi, Alessandra, Nuzzolo, Eugenia Rosa, Voso, Maria Teresa, Teofili, Luciana, Larocca, Luigi Maria, Martini, Maurizio (ORCID:0000-0002-6260-6310), Teofili, Luciana (ORCID:0000-0002-7214-1561), Larocca, Luigi Maria (ORCID:0000-0003-1739-4758), Martini, Maurizio, Capodimonti, Sara, Iachininoto, Maria Grazia, Cocomazzi, Alessandra, Nuzzolo, Eugenia Rosa, Voso, Maria Teresa, Teofili, Luciana, Larocca, Luigi Maria, Martini, Maurizio (ORCID:0000-0002-6260-6310), Teofili, Luciana (ORCID:0000-0002-7214-1561), and Larocca, Luigi Maria (ORCID:0000-0003-1739-4758)

Abstract

NA

Published
2015

10. Defective WNT Signaling and Genetic Profile Of Endothelial Cells In Patients With Low Risk Myelodysplastic Syndromes Suggest a Contribution Of Vascular Niches To Myelodysplasia

Author

Teofili, Luciana, Martini, Maurizio, Nuzzolo, Eugenia Rosa, Capodimonti, Sara, Cocomazzi, Alessandra, Iachininoto, Maria Grazia, Voso, Maria Teresa, Zini Tanzi, Gina, Larocca, Luigi Maria, Teofili, Luciana (ORCID:0000-0002-7214-1561), Martini, Maurizio (ORCID:0000-0002-6260-6310), Zini, Gina (ORCID:0000-0002-8208-066X), Larocca, Luigi Maria (ORCID:0000-0003-1739-4758), Teofili, Luciana, Martini, Maurizio, Nuzzolo, Eugenia Rosa, Capodimonti, Sara, Cocomazzi, Alessandra, Iachininoto, Maria Grazia, Voso, Maria Teresa, Zini Tanzi, Gina, Larocca, Luigi Maria, Teofili, Luciana (ORCID:0000-0002-7214-1561), Martini, Maurizio (ORCID:0000-0002-6260-6310), Zini, Gina (ORCID:0000-0002-8208-066X), and Larocca, Luigi Maria (ORCID:0000-0003-1739-4758)

Abstract

Defective WNT Signaling and Genetic Profile Of Endothelial Cells In Patients With Low Risk Myelodysplastic Syndromes Suggest a Contribution Of Vascular Niches To Myelodysplasia

Published
2013

11. Effect of antiviral therapy on pro-angiogenic hematopoietic and endothelial progenitor cells in HIV-infected people

Author

Vecchiet, J, Iachininoto, Maria Grazia, Capodimonti, Sara, Nuzzolo, Eugenia Rosa, Falasca, K, Martini, Maurizio, Mancino, P, Bianchi, M, Leone, Am, Ucciferri, C, Larocca, Luigi Maria, Teofili, Luciana, Martini, Maurizio (ORCID:0000-0002-6260-6310), Larocca, Luigi Maria (ORCID:0000-0003-1739-4758), Teofili, Luciana (ORCID:0000-0002-7214-1561), Vecchiet, J, Iachininoto, Maria Grazia, Capodimonti, Sara, Nuzzolo, Eugenia Rosa, Falasca, K, Martini, Maurizio, Mancino, P, Bianchi, M, Leone, Am, Ucciferri, C, Larocca, Luigi Maria, Teofili, Luciana, Martini, Maurizio (ORCID:0000-0002-6260-6310), Larocca, Luigi Maria (ORCID:0000-0003-1739-4758), and Teofili, Luciana (ORCID:0000-0002-7214-1561)

Abstract

BACKGROUND: HIV infection is an independent risk factor for cardiovascular disease. HIV-sustained impairment of endothelial progenitor cells (EPCs) could contribute to this process, so that it is important to assess whether antiviral therapy (ART) is able to revert these abnormalities. METHODS: We quantified in 21 naïves and 34 treated patients two functionally distinct clonogenic progenitors which have been acknowledged important for vascular repair: the hematopoietic progenitor colony forming unit - endothelial cells (CFU-EC) and the true endothelial progenitor, the endothelial colony forming cells (ECFC). We correlated results obtained with conventional vascular risk factors and with HIV-related parameters. RESULTS: We found that these progenitors behaved differently in naive and treated patients. In particular, CFU-EC level was significantly low in all naive patients and slowly recovered during ART. In contrast, the ECFC level was abnormally high in naive patients while it decreased upon ART. The CFU-EC level was related to conventional cardiovascular risk factors, as reported in general population, but also to inflammatory indexes and CD4 cell count. In contrast, the ECFC number was exclusively related to viral replication activity and to CD4 cell count. CONCLUSIONS: In HIV-infected people, the levels of CFU-EC and ECFC are related to classical cardiovascular risk factors but, in addition, they are also significantly influenced by the infection itself and by antiviral therapy.

Published
2013

12. Short Communication: Proangiogenic Hematopoietic Cells In Acute HIV Infection

Author

Falasca, K, Ucciferri, C, Teofili, Luciana, Iachininoto, Maria Grazia, Capodimonti, Sara, Nuzzolo, Eugenia Rosa, Martini, Maurizio, Bianchi, Maria, Vignale, F, Pizzigallo, E, Vecchiet, J., Teofili, Luciana (ORCID:0000-0002-7214-1561), Martini, Maurizio (ORCID:0000-0002-6260-6310), Falasca, K, Ucciferri, C, Teofili, Luciana, Iachininoto, Maria Grazia, Capodimonti, Sara, Nuzzolo, Eugenia Rosa, Martini, Maurizio, Bianchi, Maria, Vignale, F, Pizzigallo, E, Vecchiet, J., Teofili, Luciana (ORCID:0000-0002-7214-1561), and Martini, Maurizio (ORCID:0000-0002-6260-6310)

Abstract

Chronic HIV infection induces significant changes in the trafficking of circulating endothelial progenitor cells (EPCs). Specifically, it causes marked depletion of proangiogenic hematopoietic cells, the so-called colony-forming unit-endothelial cells (CFU-ECs). In this study we evaluated CFU-ECs in two subjects with acute HIV infection. We found that both patients already had a low CFU-EC level at the time of diagnosis. Nevertheless, after 6 months of antiretroviral therapy, the CFU-EC concentration reverted to normal values in both cases. HIV significantly depletes the CFU-EC compartment even in the early phase of infection, while 6-month therapy appears to be able to restore it.

Published
2013

13. Endothelial progenitor cells in HIV-positive patients: what are we talking about?

Author

Teofili, Luciana, Nuzzolo, Eugenia Rosa, Vecchiet, J, Falasca, K, Iachininoto, Maria Grazia, Martini, Maurizio, Larocca, Luigi Maria, Teofili, Luciana (ORCID:0000-0002-7214-1561), Martini, Maurizio (ORCID:0000-0002-6260-6310), Larocca, Luigi Maria (ORCID:0000-0003-1739-4758), Teofili, Luciana, Nuzzolo, Eugenia Rosa, Vecchiet, J, Falasca, K, Iachininoto, Maria Grazia, Martini, Maurizio, Larocca, Luigi Maria, Teofili, Luciana (ORCID:0000-0002-7214-1561), Martini, Maurizio (ORCID:0000-0002-6260-6310), and Larocca, Luigi Maria (ORCID:0000-0003-1739-4758)

Abstract

The contribution of EPC dysfunction to the pathogenesis of HIV-related cardiovascular diseases is an important topic, considering that both the virus and ART can affects this cell population. In this manuscript we highlight the discordance among results of studies addressing this issue and suggest that immunophenotype-definition of endothelail progenitor cells does not reflect accurately the true number of these progenitors.

Published
2013

14. Defective WNT Signaling and Genetic Profile Of Endothelial Cells In Patients With Low Risk Myelodysplastic Syndromes Suggest a Contribution Of Vascular Niches To Myelodysplasia

Author

Teofili, Luciana, primary, Martini, Maurizio, additional, Nuzzolo, Eugenia Rosa, additional, Capodimonti, Sara, additional, Cocomazzi, Alessandra, additional, Iachininoto, Maria Grazia, additional, Voso, Maria Teresa, additional, Zini, Gina, additional, and Larocca, Luigi M., additional

Published
2013
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19. von Hippel-Lindau Disease and Erythrocytosis

Author

Capodimonti, Sara, primary, Teofili, Luciana, additional, Martini, Maurizio, additional, Cenci, Tonia, additional, Iachininoto, Maria Grazia, additional, Nuzzolo, Eugenia Rosa, additional, Bianchi, Maria, additional, Murdolo, Marina, additional, Leone, Giuseppe, additional, and Larocca, Luigi M., additional

Published
2012
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21. A three-step pathway comprising PLZF/miR-146a/CXCR4 controls megakaryopoiesis

Author

Labbaye, Catherine, primary, Spinello, Isabella, additional, Quaranta, Maria Teresa, additional, Pelosi, Elvira, additional, Pasquini, Luca, additional, Petrucci, Eleonora, additional, Biffoni, Mauro, additional, Nuzzolo, Eugenia Rosa, additional, Billi, Monia, additional, Foà, Robin, additional, Brunetti, Ercole, additional, Grignani, Francesco, additional, Testa, Ugo, additional, and Peschle, Cesare, additional

Published
2008
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22. Cyclooxygenase-2 (COX-2) Inhibition Constrains Indoleamine 2,3-Dioxygenase 1 (IDO1) Activity in Acute Myeloid Leukaemia Cells.

Author

Iachininoto, Maria Grazia, Nuzzolo, Eugenia Rosa, Bonanno, Giuseppina, Mariotti, Andrea, Procoli, Annabella, Locatelli, Franco, De Cristofaro, Raimondo, and Rutella, Sergio

Subjects
*CYCLOOXYGENASE 2 inhibitors, *INDOLEAMINE 2,3-dioxygenase, *ACUTE myeloid leukemia, *PROSTAGLANDIN synthesis, *INTERLEUKIN-18, *NIMESULIDE, *GENETICS
Abstract

Indoleamine 2,3-dioxygenase 1 (IDO1) metabolizes L-tryptophan to kynurenines (KYN), inducing T-cell suppression either directly or by altering antigen-presenting-cell function. Cyclooxygenase (COX)-2, the rate-limiting enzyme in the synthesis of prostaglandins, is over-expressed by several tumours. We aimed at determining whether COX-2 inhibitors down-regulate the IFN-g-induced expression of IDO1 in acute myeloid leukaemia (AML) cells. IFN-γ at 100 ng/mL up-regulated COX-2 and IDO1 in HL-60 AML cells, both at mRNA and protein level. The increased COX-2 and IDO1 expression correlated with heightened production of prostaglandin (PG)E2 and kynurenines, respectively. Nimesulide, a preferential COX-2 inhibitor, down-regulated IDO1 mRNA/protein and attenuated kynurenine synthesis, suggesting that overall IDO inhibition resulted both from reduced IDO1 gene transcription and from inhibited IDO1 catalytic activity. From a functional standpoint, IFN-g-challenged HL-60 cells promoted the in vitro conversion of allogeneic CD4+CD25- T cells into bona fide CD4+CD25+FoxP3+ regulatory T cells, an effect that was significantly reduced by treatment of IFN-γ-activated HL-60 cells with nimesulide. Overall, these data point to COX-2 inhibition as a potential strategy to be pursued with the aim at circumventing leukaemia-induced, IDO-mediated immune dysfunction. [ABSTRACT FROM AUTHOR]

Published
2013
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23. Endothelial progenitor cells are clonal and exhibit the JAK2V617Fmutation in a subset of thrombotic patients with Ph-negative myeloproliferative neoplasms

Author

Teofili, Luciana, Martini, Maurizio, Iachininoto, Maria Grazia, Capodimonti, Sara, Nuzzolo, Eugenia Rosa, Torti, Lorenza, Cenci, Tonia, Larocca, Luigi Maria, and Leone, Giuseppe

Abstract

In this study we investigated whether neoplastic transformation occurring in Philadelphia (Ph)–negative myeloproliferative neoplasms (MPNs) could involve also the endothelial cell compartment. We evaluated the level of endothelial colony-forming cells (E-CFCs) in 42 patients (15 with polycythemia vera, 12 with essential thrombocythemia, and 15 with primary myelofibrosis). All patients had 1 molecular abnormality (JAK2V617For MPLW515Kmutations, SOCSgene hypermethylation, clonal pattern of growth) detectable in their granulocytes. The growth of colonies was obtained in 22 patients and, among them, patients with primary myelofibrosis exhibited the highest level of E-CFCs. We found that E-CFCs exhibited no molecular abnormalities in12 patients, had SOCSgene hypermethylation, were polyclonal at human androgen receptor analysis in 5 patients, and resulted in JAK2V617Fmutated and clonal in 5 additional patients, all experiencing thrombotic complications. On the whole, patients with altered E-CFCs required antiproliferative therapy more frequently than patients with normal E-CFCs. Moreover JAK2V617F-positive E-CFCs showed signal transducer and activator of transcription 5 and 3 phosphorylation rates higher than E-CFCs isolated from healthy persons and patients with MPN without molecular abnormalities. Finally, JAK2V617F-positive E-CFCs exhibited a high proficiency to adhere to normal mononuclear cells. This study highlights a novel mechanism underlying the thrombophilia observed in MPN.

Published
2011
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24. Cord Blood Platelet Lysate: In Vitro Evaluation to Support the Use in Regenerative Medicine.

Author

Valentini CG, Nuzzolo ER, Bianchi M, Orlando N, Iachininoto MG, Pinci P, and Teofili L

Abstract

Platelet-rich plasma (PRP) is an inexpensive and safe substitute of recombinant growth factors in vitro and in vivo . Due to its putative effect on tissue repair, the use of autologous PRP has become largely popular. Recently, a jellified PRP derivative obtained from umbilical cord blood (CB) has been utilized in vivo to treat mucosal and cutaneous lesions. Nevertheless, whether PRP derived from CB and adult blood display different potency in promoting cell growth in vitro has been rarely investigated. In this study, we compared cytokine profile and mesenchymal cell growth supporting the ability of platelet lysate obtained from adult and cord blood. Our in vitro results strongly back the utilization of CB platelet lysate in vivo , as an efficacious, safe and inexpensive alternative to promote damaged tissue healing when the autologous PRP is contraindicated. Moreover, the policy of manufacturing CB platelet lysate can limit the current disposal of many collected CB units not suitable for transplant due to their low nucleated cell count., Competing Interests: Competing interests: The authors have declared that no competing interests exist.

Published
2019
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