26 results on '"Nuttapat Anuwongcharoen"'
Search Results
2. Enhancing the activity of β-lactamase inhibitory protein-II with cell-penetrating peptide against KPC-2-carrying Klebsiella pneumoniae
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Chawalit Chatupheeraphat, Jiratchaya Peamchai, Noramon Kaewsai, Nuttapat Anuwongcharoen, and Warawan Eiamphungporn
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Medicine ,Science - Published
- 2024
3. Machine learning approaches to study the structure-activity relationships of LpxC inhibitors
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Tianshi Yu, Li Chuin Chong, Chanin Nantasenamat, Nuttapat Anuwongcharoen, and Theeraphon Piacham
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antimicrobial resistance ,lpxc ,qsar ,machine learning ,cheminformatics ,activity cliff ,chemotype ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Biology (General) ,QH301-705.5 - Abstract
Antimicrobial resistance (AMR) has emerged as one of the global threats to human health in the 21st century. Drug discovery of inhibitors against novel targets rather than conventional bacterial targets has been considered an inevitable strategy for the growing threat of AMR infections. In this study, we applied quantitative structure-activity relationship (QSAR) modeling to the LpxC inhibitors to predict the inhibitory activity. In addition, we performed various cheminformatics analysis consisting of the exploration of the chemical space, identification of chemotypes, performing structure-activity landscape and activity cliffs as well as construction of the Structure-Activity Similarity (SAS) map. We built a total of 24 QSAR classification models using PubChem and MACCS fingerprint with 12 various machine learning algorithms. The best model with PubChem fingerprint is the Extremely Gradient Boost model (accuracy on the training set: 0.937; accuracy on the 10-fold cross-validation set: 0.795; accuracy on the test set: 0.799). Furthermore, it was found that the best model using the MACCS fingerprint was the Random Forest model (accuracy on the training set: 0.955; accuracy on the 10-fold cross-validation set: 0.803; accuracy on the test set: 0.785). In addition, we have identified eight consensus activity cliff generators that are highly informative for further SAR investigations. It is hoped that findings presented herein can provide guidance for further lead optimization of LpxC inhibitors.
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- 2023
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4. DeepAR: a novel deep learning-based hybrid framework for the interpretable prediction of androgen receptor antagonists
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Nalini Schaduangrat, Nuttapat Anuwongcharoen, Phasit Charoenkwan, and Watshara Shoombuatong
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Androgen receptors ,QSAR ,Cheminformatics ,Machine learning ,Deep learning ,Bioinformatics ,Information technology ,T58.5-58.64 ,Chemistry ,QD1-999 - Abstract
Abstract Drug resistance represents a major obstacle to therapeutic innovations and is a prevalent feature in prostate cancer (PCa). Androgen receptors (ARs) are the hallmark therapeutic target for prostate cancer modulation and AR antagonists have achieved great success. However, rapid emergence of resistance contributing to PCa progression is the ultimate burden of their long-term usage. Hence, the discovery and development of AR antagonists with capability to combat the resistance, remains an avenue for further exploration. Therefore, this study proposes a novel deep learning (DL)-based hybrid framework, named DeepAR, to accurately and rapidly identify AR antagonists by using only the SMILES notation. Specifically, DeepAR is capable of extracting and learning the key information embedded in AR antagonists. Firstly, we established a benchmark dataset by collecting active and inactive compounds against AR from the ChEMBL database. Based on this dataset, we developed and optimized a collection of baseline models by using a comprehensive set of well-known molecular descriptors and machine learning algorithms. Then, these baseline models were utilized for creating probabilistic features. Finally, these probabilistic features were combined and used for the construction of a meta-model based on a one-dimensional convolutional neural network. Experimental results indicated that DeepAR is a more accurate and stable approach for identifying AR antagonists in terms of the independent test dataset, by achieving an accuracy of 0.911 and MCC of 0.823. In addition, our proposed framework is able to provide feature importance information by leveraging a popular computational approach, named SHapley Additive exPlanations (SHAP). In the meanwhile, the characterization and analysis of potential AR antagonist candidates were achieved through the SHAP waterfall plot and molecular docking. The analysis inferred that N-heterocyclic moieties, halogenated substituents, and a cyano functional group were significant determinants of potential AR antagonists. Lastly, we implemented an online web server by using DeepAR (at http://pmlabstack.pythonanywhere.com/DeepAR ). We anticipate that DeepAR could be a useful computational tool for community-wide facilitation of AR candidates from a large number of uncharacterized compounds.
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- 2023
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5. Towards combating antibiotic resistance by exploring the quantitative structure-activity relationship of NDM-1 inhibitors
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Tianshi Yu, Aijaz Ahmad Malik, Nuttapat Anuwongcharoen, Warawan Eiamphungporn, Chanin Nantasenamat, and Theeraphon Piacham
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antibiotic resistance ,beta-lactamase ,ndm-1 ,qsar ,drug discovery ,data science ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Biology (General) ,QH301-705.5 - Abstract
The emergence of New Delhi metallo-beta-lactamase-1 (NDM-1) has conferred enteric bacteria resistance to almost all beta-lactam antibiotics. Its capability of horizontal transfer through plasmids, amongst humans, animal reservoirs and the environment, has added up to the totality of antimicrobial resistance control, animal husbandry and food safety. Thus far, there have been no effective drugs for neutralizing NDM-1. This study explores the structure-activity relationship of NDM-1 inhibitors. IC50 values of NDM-1 inhibitors were compiled from both the ChEMBL database and literature. After curation, a final set of 686 inhibitors were used for machine learning model building using the random forest algorithm against 12 sets of molecular fingerprints. Benchmark results indicated that the KlekotaRothCount fingerprint provided the best overall performance with an accuracy of 0.978 and 0.778 for the training and testing set, respectively. Model interpretation revealed that nitrogen-containing features (KRFPC 4080, KRFPC 3882, KRFPC 677, KRFPC 3608, KRFPC 3750, KRFPC 4287 and KRFPC 3943), sulfur-containing substructures (KRFPC 2855 and KRFPC 4843), aromatic features (KRFPC 1566, KRFPC 1564, KRFPC 1642, KRFPC 3608, KRFPC 4287 and KRFPC 3943), carbonyl features (KRFPC 1193 and KRFPC 3025), aliphatic features (KRFPC 2975, KRFPC 297, KRFPC 3224 and KRFPC 669) are features contributing to NDM-1 inhibitory activity. It is anticipated that findings from this study would help facilitate the drug discovery of NDM-1 inhibitors by providing guidelines for further lead optimization.
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- 2022
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6. StackPR is a new computational approach for large-scale identification of progesterone receptor antagonists using the stacking strategy
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Nalini Schaduangrat, Nuttapat Anuwongcharoen, Mohammad Ali Moni, Pietro Lio’, Phasit Charoenkwan, and Watshara Shoombuatong
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Medicine ,Science - Abstract
Abstract Progesterone receptors (PRs) are implicated in various cancers since their presence/absence can determine clinical outcomes. The overstimulation of progesterone can facilitate oncogenesis and thus, its modulation through PR inhibition is urgently needed. To address this issue, a novel stacked ensemble learning approach (termed StackPR) is presented for fast, accurate, and large-scale identification of PR antagonists using only SMILES notation without the need for 3D structural information. We employed six popular machine learning (ML) algorithms (i.e., logistic regression, partial least squares, k-nearest neighbor, support vector machine, extremely randomized trees, and random forest) coupled with twelve conventional molecular descriptors to create 72 baseline models. Then, a genetic algorithm in conjunction with the self-assessment-report approach was utilized to determine m out of the 72 baseline models as means of developing the final meta-predictor using the stacking strategy and tenfold cross-validation test. Experimental results on the independent test dataset show that StackPR achieved impressive predictive performance with an accuracy of 0.966 and Matthew’s coefficient correlation of 0.925. In addition, analysis based on the SHapley Additive exPlanation algorithm and molecular docking indicates that aliphatic hydrocarbons and nitrogen-containing substructures were the most important features for having PR antagonist activity. Finally, we implemented an online webserver using StackPR, which is freely accessible at http://pmlabstack.pythonanywhere.com/StackPR . StackPR is anticipated to be a powerful computational tool for the large-scale identification of unknown PR antagonist candidates for follow-up experimental validation.
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- 2022
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7. Exploring the Chemical Space of CYP17A1 Inhibitors Using Cheminformatics and Machine Learning
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Tianshi Yu, Tianyang Huang, Leiye Yu, Chanin Nantasenamat, Nuttapat Anuwongcharoen, Theeraphon Piacham, Ruobing Ren, and Ying-Chih Chiang
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CYP17A1 ,prostate cancer ,cheminformatics ,quantitative structure–activity relationship ,Murcko scaffold ,Organic chemistry ,QD241-441 - Abstract
Cytochrome P450 17A1 (CYP17A1) is one of the key enzymes in steroidogenesis that produces dehydroepiandrosterone (DHEA) from cholesterol. Abnormal DHEA production may lead to the progression of severe diseases, such as prostatic and breast cancers. Thus, CYP17A1 is a druggable target for anti-cancer molecule development. In this study, cheminformatic analyses and quantitative structure–activity relationship (QSAR) modeling were applied on a set of 962 CYP17A1 inhibitors (i.e., consisting of 279 steroidal and 683 nonsteroidal inhibitors) compiled from the ChEMBL database. For steroidal inhibitors, a QSAR classification model built using the PubChem fingerprint along with the extra trees algorithm achieved the best performance, reflected by the accuracy values of 0.933, 0.818, and 0.833 for the training, cross-validation, and test sets, respectively. For nonsteroidal inhibitors, a systematic cheminformatic analysis was applied for exploring the chemical space, Murcko scaffolds, and structure–activity relationships (SARs) for visualizing distributions, patterns, and representative scaffolds for drug discoveries. Furthermore, seven total QSAR classification models were established based on the nonsteroidal scaffolds, and two activity cliff (AC) generators were identified. The best performing model out of these seven was model VIII, which is built upon the PubChem fingerprint along with the random forest algorithm. It achieved a robust accuracy across the training set, the cross-validation set, and the test set, i.e., 0.96, 0.92, and 0.913, respectively. It is anticipated that the results presented herein would be instrumental for further CYP17A1 inhibitor drug discovery efforts.
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- 2023
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8. Probing the origins of human acetylcholinesterase inhibition via QSAR modeling and molecular docking
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Saw Simeon, Nuttapat Anuwongcharoen, Watshara Shoombuatong, Aijaz Ahmad Malik, Virapong Prachayasittikul, Jarl E.S. Wikberg, and Chanin Nantasenamat
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Acetylcholinesterase ,Acetylcholinesterase inhibitor ,Alzheimer’s disease ,Dementia ,Neurodegenerative disease ,Quantitative structure-activity relationship ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Alzheimer’s disease (AD) is a chronic neurodegenerative disease which leads to the gradual loss of neuronal cells. Several hypotheses for AD exists (e.g., cholinergic, amyloid, tau hypotheses, etc.). As per the cholinergic hypothesis, the deficiency of choline is responsible for AD; therefore, the inhibition of AChE is a lucrative therapeutic strategy for the treatment of AD. Acetylcholinesterase (AChE) is an enzyme that catalyzes the breakdown of the neurotransmitter acetylcholine that is essential for cognition and memory. A large non-redundant data set of 2,570 compounds with reported IC50 values against AChE was obtained from ChEMBL and employed in quantitative structure-activity relationship (QSAR) study so as to gain insights on their origin of bioactivity. AChE inhibitors were described by a set of 12 fingerprint descriptors and predictive models were constructed from 100 different data splits using random forest. Generated models afforded R2, ${Q}_{\mathrm{CV }}^{2}$ Q CV 2 and ${Q}_{\mathrm{Ext}}^{2}$ Q Ext 2 values in ranges of 0.66–0.93, 0.55–0.79 and 0.56–0.81 for the training set, 10-fold cross-validated set and external set, respectively. The best model built using the substructure count was selected according to the OECD guidelines and it afforded R2, ${Q}_{\mathrm{CV }}^{2}$ Q CV 2 and ${Q}_{\mathrm{Ext}}^{2}$ Q Ext 2 values of 0.92 ± 0.01, 0.78 ± 0.06 and 0.78 ± 0.05, respectively. Furthermore, Y-scrambling was applied to evaluate the possibility of chance correlation of the predictive model. Subsequently, a thorough analysis of the substructure fingerprint count was conducted to provide informative insights on the inhibitory activity of AChE inhibitors. Moreover, Kennard–Stone sampling of the actives were applied to select 30 diverse compounds for further molecular docking studies in order to gain structural insights on the origin of AChE inhibition. Site-moiety mapping of compounds from the diversity set revealed three binding anchors encompassing both hydrogen bonding and van der Waals interaction. Molecular docking revealed that compounds 13, 5 and 28 exhibited the lowest binding energies of −12.2, −12.0 and −12.0 kcal/mol, respectively, against human AChE, which is modulated by hydrogen bonding, π–π stacking and hydrophobic interaction inside the binding pocket. These information may be used as guidelines for the design of novel and robust AChE inhibitors.
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- 2016
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9. Origin of aromatase inhibitory activity via proteochemometric modeling
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Saw Simeon, Ola Spjuth, Maris Lapins, Sunanta Nabu, Nuttapat Anuwongcharoen, Virapong Prachayasittikul, Jarl E.S. Wikberg, and Chanin Nantasenamat
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Aromatase ,Quantitative structure–activity relationship ,Breast cancer ,Data mining ,QSAR ,Aromatase inhibitor ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Aromatase, the rate-limiting enzyme that catalyzes the conversion of androgen to estrogen, plays an essential role in the development of estrogen-dependent breast cancer. Side effects due to aromatase inhibitors (AIs) necessitate the pursuit of novel inhibitor candidates with high selectivity, lower toxicity and increased potency. Designing a novel therapeutic agent against aromatase could be achieved computationally by means of ligand-based and structure-based methods. For over a decade, we have utilized both approaches to design potential AIs for which quantitative structure–activity relationships and molecular docking were used to explore inhibitory mechanisms of AIs towards aromatase. However, such approaches do not consider the effects that aromatase variants have on different AIs. In this study, proteochemometrics modeling was applied to analyze the interaction space between AIs and aromatase variants as a function of their substructural and amino acid features. Good predictive performance was achieved, as rigorously verified by 10-fold cross-validation, external validation, leave-one-compound-out cross-validation, leave-one-protein-out cross-validation and Y-scrambling tests. The investigations presented herein provide important insights into the mechanisms of aromatase inhibitory activity that could aid in the design of novel potent AIs as breast cancer therapeutic agents.
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- 2016
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10. Exploring the chemical space of influenza neuraminidase inhibitors
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Nuttapat Anuwongcharoen, Watshara Shoombuatong, Tanawut Tantimongcolwat, Virapong Prachayasittikul, and Chanin Nantasenamat
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Influenza ,Neuraminidase ,Neuraminidase inhibitor ,Chemical space ,QSAR ,Scaffold analysis ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
The fight against the emergence of mutant influenza strains has led to the screening of an increasing number of compounds for inhibitory activity against influenza neuraminidase. This study explores the chemical space of neuraminidase inhibitors (NAIs), which provides an opportunity to obtain further molecular insights regarding the underlying basis of their bioactivity. In particular, a large set of 347 and 175 NAIs against influenza A and B, respectively, was compiled from the literature. Molecular and quantum chemical descriptors were obtained from low-energy conformational structures geometrically optimized at the PM6 level. The bioactivities of NAIs were classified as active or inactive according to their half maximum inhibitory concentration (IC50) value in which IC50 < 1µM and ≥ 10µM were defined as active and inactive compounds, respectively. Interpretable decision rules were derived from a quantitative structure–activity relationship (QSAR) model established using a set of substructure descriptors via decision tree analysis. Univariate analysis, feature importance analysis from decision tree modeling and molecular scaffold analysis were performed on both data sets for discriminating important structural features amongst active and inactive NAIs. Good predictive performance was achieved as deduced from accuracy and Matthews correlation coefficient values in excess of 81% and 0.58, respectively, for both influenza A and B NAIs. Furthermore, molecular docking was employed to investigate the binding modes and their moiety preferences of active NAIs against both influenza A and B neuraminidases. Moreover, novel NAIs with robust binding fitness towards influenza A and B neuraminidase were generated via combinatorial library enumeration and their binding fitness was on par or better than FDA-approved drugs. The results from this study are anticipated to be beneficial for guiding the rational drug design of novel NAIs for treating influenza infections.
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- 2016
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11. Roles of d-Amino Acids on the Bioactivity of Host Defense Peptides
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Hao Li, Nuttapat Anuwongcharoen, Aijaz Ahmad Malik, Virapong Prachayasittikul, Jarl E. S. Wikberg, and Chanin Nantasenamat
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d-amino+acid%22">">d-amino acid ,host defense peptide ,antimicrobial peptide ,anticancer peptide ,diastereomer ,HDP ,AMP ,bioactivity ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Host defense peptides (HDPs) are positively-charged and amphipathic components of the innate immune system that have demonstrated great potential to become the next generation of broad spectrum therapeutic agents effective against a vast array of pathogens and tumor. As such, many approaches have been taken to improve the therapeutic efficacy of HDPs. Amongst these methods, the incorporation of d-amino acids (d-AA) is an approach that has demonstrated consistent success in improving HDPs. Although, virtually all HDP review articles briefly mentioned about the role of d-AA, however it is rather surprising that no systematic review specifically dedicated to this topic exists. Given the impact that d-AA incorporation has on HDPs, this review aims to fill that void with a systematic discussion of the impact of d-AA on HDPs.
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- 2016
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12. Cheminformatic Analysis and Machine Learning Modeling to Investigate Androgen Receptor Antagonists to Combat Prostate Cancer
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Tianshi Yu, Chanin Nantasenamat, Supicha Kachenton, Nuttapat Anuwongcharoen, and Theeraphon Piacham
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General Chemical Engineering ,General Chemistry - Published
- 2023
13. osFP: a web server for predicting the oligomeric states of fluorescent proteins.
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Saw Simeon, Watshara Shoombuatong, Nuttapat Anuwongcharoen, Likit Preeyanon, Virapong Prachayasittikul, Jarl E. S. Wikberg, and Chanin Nantasenamat
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- 2016
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14. In Silico Approaches for the Prediction and Analysis of Antiviral Peptides: A Review
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Watshara Shoombuatong, Md. Mehedi Hasan, Nuttapat Anuwongcharoen, Chanin Nantasenamat, and Phasit Charoenkwan
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Pharmacology ,0303 health sciences ,Drug discovery ,Computer science ,In silico ,Feature selection ,Computational biology ,01 natural sciences ,Antiviral Agents ,0104 chemical sciences ,Support vector machine ,Machine Learning ,010404 medicinal & biomolecular chemistry ,03 medical and health sciences ,Robust learning ,Drug Discovery ,Feature (machine learning) ,Humans ,Computer Simulation ,Peptides ,Algorithms ,030304 developmental biology - Abstract
In light of the growing resistance toward current antiviral drugs, efforts to discover novel and effective antiviral therapeutic agents remain a pressing scientific effort. Antiviral peptides (AVPs) represent promising therapeutic agents due to their extraordinary advantages in terms of potency, efficacy and pharmacokinetic properties. The growing volume of newly discovered peptide sequences in the post-genomic era requires computational approaches for timely and accurate identification of AVPs. Machine learning (ML) methods such as random forest and support vector machine represent robust learning algorithms that are instrumental in successful peptide-based drug discovery. Therefore, this review summarizes the current state-of-the-art application of ML methods for identifying AVPs directly from the sequence information. We compare the efficiency of these methods in terms of the underlying characteristics of the dataset used along with feature encoding methods, ML algorithms, cross-validation methods and prediction performance. Finally, guidelines for the development of robust AVP models are also discussed. It is anticipated that this review will serve as a useful guide for the design and development of robust AVP and related therapeutic peptide predictors in the future.
- Published
- 2020
15. Privileged substructures for anti-sickling activity via cheminformatic analysis
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Virapong Prachayasittikul, Nuttapat Anuwongcharoen, Watshara Shoombuatong, Chuleeporn Phanus-umporn, Chanin Nantasenamat, and Veda Prachayasittikul
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0301 basic medicine ,03 medical and health sciences ,030104 developmental biology ,Chemistry ,General Chemical Engineering ,Severe pain ,Aromaticity ,General Chemistry ,Computational biology ,Matthews correlation coefficient ,World health ,Therapeutic strategy - Abstract
Sickle cell disease (SCD), an autosomal recessive genetic disorder, has been recognized by the World Health Organization (WHO) as a major public health problem as it affects 300 000 individuals worldwide. Complications arising from SCD include anemia, microvascular occlusion, severe pain, stokes, renal dysfunction and infections. A lucrative therapeutic strategy is to employ anti-sickling agents that can disrupt the formation of the HbS polymer. This study therefore employed cheminformatic approaches, encompassing classification structure–activity relationship (CSAR) modeling, to deduce the privileged substructures giving rise to the anti-sickling activity of an investigated set of 115 compounds, followed by substructure analysis. Briefly, the compiled compounds were described by fingerprint descriptors and used in the construction of CSAR models via several machine learning algorithms. The modelability of the data set, as exemplified by the MODI index, was determined to be in the range of 0.70–0.84. The predictive performance was deduced by the accuracy, sensitivity, specificity and Matthews correlation coefficient, which was found to be statistically robust, whereby the former three parameters afforded values in excess of 0.7 while the latter statistical parameter provided a value greater than 0.5. An analysis of the top 20 important substructure descriptors for anti-sickling activity revealed that 10 important features were significant in the differentiation of actives from inactives, as illustrated by aromaticity/conjugation (e.g. SubFPC287, SubFPC171 and SubFPC5), carbonyl groups (e.g. SubFPC137, SubFPC139, SubFPC49 and SubFPC135) and miscellaneous groups (e.g. SubFPC303, SubFPC302 and SubFPC275). Furthermore, an analysis of the structure–activity relationship revealed that the length of alkyl chains, choice of functional moiety and position of substitution on the benzene ring may affect the anti-sickling activity of these compounds. Thus, this knowledge is anticipated to be useful for guiding the design of robust compounds against the gelling activity of HbS, as preliminarily demonstrated in the data-driven compound design presented herein.
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- 2018
16. Proteochemometric Modeling for Drug Repositioning
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Jarl E. S. Wikberg, Nuttapat Anuwongcharoen, Chuleeporn Phanus-umporn, Nalini Schaduangrat, Nagaya Sriwanichpoom, and Chanin Nantasenamat
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Drug ,Drug repositioning ,Quantitative structure–activity relationship ,Drug development ,Computer science ,Drug discovery ,media_common.quotation_subject ,Target protein ,Polypharmacology ,Computational biology ,Repurposing ,media_common - Abstract
The rising cost of drug development has necessitated the search for efficient approaches for the discovery of novel drugs. Such difficulty arises from the fact that promising lead compounds would need to be further scrutinized via preclinical and clinical studies so as to characterize their absorption, distribution, metabolism, excretion, and toxicity in order to verify their safety for clinical use. In this respect, drug repositioning/repurposing represents an attractive venue for the discovery of novel therapeutic indications for existing food and drug administration-approved drugs, which in a nutshell repurposes old drugs for treating new diseases for which they were not originally developed. Computational approaches have been instrumental in drug repositioning efforts as they make use of existing biological and chemical data to uncover new therapeutic indications by relying on the concept that similar compounds bind to similar targets. Quantitative structure-activity relationship (QSAR) is a powerful ligand-based approach that correlates structural features of compounds with their observed biological activity against a single target protein. Proteochemometric modeling (PCM) extends QSAR to an extra dimension by allowing several target proteins to be considered in a single model. This chapter briefly explores the concepts of polypharmacology and computational drug discovery and finally examines the use of PCM for drug repositioning.
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- 2019
17. Contributors
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Bashir Akhlaq Akhoon, Marta E. Alarcón-Riquelme, Lucas N. Alberca, Juan I. Alice, Nuttapat Anuwongcharoen, Kazim Yalcin Arga, Carolina L. Belllera, Vladimir P. Berishvili, Anshu Bhardwaj, Vijaya Lakshmi Bodiga, Sreedhar Bodiga, Michal Brylinski, Pedro Carmona-Sáez, Sohini Chakraborti, Vaishali Chaudhry, Lixia Chen, Mohane Selvaraj Coumar, Daniel Toro-Domínguez, Nikolas Dietis, Noelie Douanne, Dmitry Druzhilovskiy, K. Eurídice Juárez-Mercado, Christopher Fernández-Prada, Pankaj Gautam, Indira Ghosh, C. Gopi Mohan, Shozeb Haider, Li Hua, Jameel Iqbal, Nivya James, Bani Jolly, Prashant S. Kharkar, Se-Min Kim, Shivani Kumar, Suresh Kumar, Pawan Kumar, Lukasz Kurgan, Yu-Chen Lo, Edgar López-López, Janvhi S. Machhar, K. Manzoor, José L. Medina-Franco, Anu R. Melge, George Minadakis, Aida Minguez-Menendez, Makedonka Mitreva, Rubens L. Monte-Neto, Gurusamy Muneeswaran, Selvaraman Nagamani, Shantikumar V. Nair, Chanin Nantasenamat, G. Narahari Sastry, Amit Nargotra, Anastasia A. Nikitina, Alexey A. Orlov, Dmitry I. Osolodkin, Anastasis Oulas, Manoj Kumar Pal, Vladimir A. Palyulin, Ashma Pandya, Anurag Passi, Joan Pena, Chuleeporn Phanus-umporn, Douglas E.V. Pires, Vladimir Poroikov, Fernando D. Prieto-Martínez, Eugene V. Radchenko, Gayatri Ramakrishnan, K. Ramanathan, Bruce A. Rosa, Rosaleen Sahoo, Niteshkumar U. Sahu, Pemra Ozbek Sarica, Sailu Sarvagalla, Kyriaki Savva, María L. Sbaraglini, Nalini Schaduangrat, Onur Serçinoğlu, Chetan P. Shah, V. Shanthi, Tina Sharma, Kleitos Sokratous, George M. Spyrou, Narayanaswamy Srinivasan, Nagaya Sriwanichpoom, Li Sun, Safiulla Basha Syed, Alan Talevi, Harshita Tiwari, Jorge Z. Torres, Luiza G. Tunes, Beste Turanli, Rahul Tyagi, Chen Wang, Jarl E.S. Wikberg, Xuhua Xia, Tony Yuen, Margarita Zachariou, Neeha Zaidi, Samir Zaidi, Mone Zaidi, Alberta Zallone, and Mengzhu Zheng
- Published
- 2019
18. Synthesis and molecular docking of N,N'-disubstituted thiourea derivatives as novel aromatase inhibitors
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Veda Prachayasittikul, Virapong Prachayasittikul, Somsak Ruchirawat, Nuttapat Anuwongcharoen, Ratchanok Pingaew, and Supaluk Prachayasittikul
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Stereochemistry ,medicine.drug_class ,Antineoplastic Agents ,01 natural sciences ,Biochemistry ,Hydrophobic effect ,chemistry.chemical_compound ,Aromatase ,Drug Discovery ,Ic50 values ,medicine ,Humans ,Androstenedione ,Molecular Biology ,IC50 ,chemistry.chemical_classification ,Aromatase inhibitor ,Binding Sites ,biology ,Molecular Structure ,010405 organic chemistry ,Aromatase Inhibitors ,Organic Chemistry ,Thiourea ,0104 chemical sciences ,Molecular Docking Simulation ,010404 medicinal & biomolecular chemistry ,Enzyme ,chemistry ,biology.protein ,MCF-7 Cells ,Hydrophobic and Hydrophilic Interactions - Abstract
A three series of thioureas, monothiourea type I (4a–g), 1,4-bisthiourea type II (5a–h) and 1,3-bisthiourea type III (6a–h) were synthesized. Their aromatase inhibitory activities have been evaluated. Interestingly, eight thiourea derivatives (4e, 5f–h, 6d, 6f–h) exhibited the aromatase inhibitory activities with IC50 range of 0.6–10.2 μM. The meta-bisthiourea bearing 4-NO2 group (6f) and 3,5-diCF3 groups (6h) were shown to be the most potent compounds with sub-micromolar IC50 values of 0.8 and 0.6 μM, respectively. Molecular docking also revealed that one of the thiourea moieties of these two compounds could mimic steroidal backbone of the natural androstenedione (ASD) via hydrophobic interactions with enzyme residues (Val370, Leu477, Thr310, and Phe221 for 6f, Val370, Leu477, Ser478, and Ile133 for 6h). This is the first time that the bisthioureas have been reported for their potential to be developed as aromatase inhibitors, in which the 4-NO2 and 3,5-diCF3 analogs have been highlighted as promising candidates.
- Published
- 2018
19. Privileged substructures for anti-sickling activity
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Chuleeporn, Phanus-Umporn, Watshara, Shoombuatong, Veda, Prachayasittikul, Nuttapat, Anuwongcharoen, and Chanin, Nantasenamat
- Abstract
Sickle cell disease (SCD), an autosomal recessive genetic disorder, has been recognized by the World Health Organization (WHO) as a major public health problem as it affects 300 000 individuals worldwide. Complications arising from SCD include anemia, microvascular occlusion, severe pain, stokes, renal dysfunction and infections. A lucrative therapeutic strategy is to employ anti-sickling agents that can disrupt the formation of the HbS polymer. This study therefore employed cheminformatic approaches, encompassing classification structure-activity relationship (CSAR) modeling, to deduce the privileged substructures giving rise to the anti-sickling activity of an investigated set of 115 compounds, followed by substructure analysis. Briefly, the compiled compounds were described by fingerprint descriptors and used in the construction of CSAR models
- Published
- 2017
20. Towards the Revival of Interpretable QSAR Models
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Chanin Nantasenamat, Apilak Worachartcheewan, Saw Simeon, Watshara Shoombuatong, Wiwat Owasirikul, Philip Prathipati, Nuttapat Anuwongcharoen, and Jarl E. S. Wikberg
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0301 basic medicine ,Quantitative structure–activity relationship ,Computer science ,Predictive capability ,Key issues ,01 natural sciences ,0104 chemical sciences ,Quantitative Structure Property Relationship ,010404 medicinal & biomolecular chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,Cheminformatics ,Chemogenomics ,Biochemical engineering ,Interpretability - Abstract
Quantitative structure-activity relationship (QSAR) has been instrumental in aiding medicinal chemists and physical scientists in understanding how modification of substituents at different positions on a molecular structure exert its influence on the observed biological activity and physicochemical property, respectively. QSAR has received great attention owing to its predictive capability and as such efforts had been directed toward obtaining models with high prediction performance. However, to be useful QSAR models need to be informative and interpretable in which the underlying molecular features that contribute to the increase or decrease of the biological activity are revealed by the model. Thus, the aim of this chapter is to briefly review the general concepts of QSAR modeling, its development and discussions on key issues influencing and contributing to the interpretability of QSAR models.
- Published
- 2017
21. Exploring the chemical space of influenza neuraminidase inhibitors
- Author
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Nuttapat Anuwongcharoen, Tanawut Tantimongcolwat, Virapong Prachayasittikul, Chanin Nantasenamat, and Watshara Shoombuatong
- Subjects
0301 basic medicine ,Quantitative structure–activity relationship ,Drugs and Devices ,Fragment analysis ,medicine.drug_class ,Bioinformatics ,lcsh:Medicine ,Drug design ,Neuraminidase ,Computational biology ,Neuraminidase inhibitor ,Biology ,01 natural sciences ,General Biochemistry, Genetics and Molecular Biology ,Computational Science ,03 medical and health sciences ,medicine ,Chemical space ,Data mining ,Combinatorial library enumeration ,Quantum chemical ,010405 organic chemistry ,QSAR ,General Neuroscience ,lcsh:R ,Computational Biology ,Influenza a ,General Medicine ,Matthews correlation coefficient ,Scaffold analysis ,Virology ,Influenza ,0104 chemical sciences ,030104 developmental biology ,Molecular docking ,biology.protein ,General Agricultural and Biological Sciences - Abstract
The fight against the emergence of mutant influenza strains has led to the screening of an increasing number of compounds for inhibitory activity against influenza neuraminidase. This study explores the chemical space of neuraminidase inhibitors (NAIs), which provides an opportunity to obtain further molecular insights regarding the underlying basis of their bioactivity. In particular, a large set of 347 and 175 NAIs against influenza A and B, respectively, was compiled from the literature. Molecular and quantum chemical descriptors were obtained from low-energy conformational structures geometrically optimized at the B3LYP/6-31G(d) level. The bioactivities of NAIs were classified as active or inactive according to their half maximum inhibitory concentration (IC50) value in which IC50 < 1 μM and > 10 μM were defined as active and inactive compounds, respectively. Interpretable decision rules were derived from a quantitative structure-activity relationship (QSAR) model established using a set of 13 descriptors via decision tree analysis. Good predictive performance was achieved as deduced from 10-fold cross-validation, in which an accuracy and MCC of 82.46% and 0.649, respectively, were obtained for influenza A NAIs while values of 80.00% and 0.553 were obtained for influenza B NAIs. Both univariate and multivariate analyses revealed the importance of the lowest unoccupied molecular orbital, number of hydrogen bond donors and number of hydrogen bond acceptors in the predictive model of NAIs against influenza A while the number of hydrogen bond acceptors, molecular energy and the energy gap between the highest occupied and lowest unoccupied molecular orbitals were important in the predictive model for influenza B NAIs. Molecular scaffold analysis was performed on both data sets for discriminating important structural features among active and inactive NAIs. Furthermore, molecular docking was employed to investigate the binding modes and their moiety preferences of active NAIs against both influenza A and B neuraminidase. Moreover, novel NAIs with robust binding fitness towards influenza A and B neuraminidase were generated via combinatorial library enumeration and their binding fitness was on par or better than FDA-approved drugs. The results from this study are anticipated to be beneficial for guiding the rational drug design of novel NAIs for treating influenza infections.
- Published
- 2015
22. Discovery of novel 1,2,3-triazole derivatives as anticancer agents using QSAR and in silico structural modification
- Author
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Chanin Nantasenamat, Veda Prachayasittikul, Ratchanok Pingaew, Supaluk Prachayasittikul, Virapong Prachayasittikul, Somsak Ruchirawat, Nuttapat Anuwongcharoen, and Apilak Worachartcheewan
- Subjects
Quantitative structure–activity relationship ,Computational chemistry ,Multidisciplinary ,1,2,3-Triazole ,Chemistry ,QSAR ,In silico ,Research ,Triazole ,Computational biology ,Triazoles ,Anticancer activity ,Drug design ,chemistry.chemical_compound ,Human health ,Structural modification ,Cancer cell lines - Abstract
Considerable attention has been given on the search for novel anticancer drugs with respect to the disease sequelae on human health and well-being. Triazole is considered to be an attractive scaffold possessing diverse biological activities. Structural modification on the privileged structures is noted as an effective strategy towards successful design and development of novel drugs. The quantitative structure–activity relationships (QSAR) is well-known as a powerful computational tool to facilitate the discovery of potential compounds. In this study, a series of thirty-two 1,2,3-triazole derivatives (1–32) together with their experimentally measured cytotoxic activities against four cancer cell lines i.e., HuCCA-1, HepG2, A549 and MOLT-3 were used for QSAR analysis. Four QSAR models were successfully constructed with acceptable predictive performance affording RCV ranging from 0.5958 to 0.8957 and RMSECV ranging from 0.2070 to 0.4526. An additional set of 64 structurally modified triazole compounds (1A–1R, 2A–2R, 7A–7R and 8A–8R) were constructed in silico and their predicted cytotoxic activities were obtained using the constructed QSAR models. The study suggested crucial moieties and certain properties essential for potent anticancer activity and highlighted a series of promising compounds (21, 28, 32, 1P, 8G, 8N and 8Q) for further development as novel triazole-based anticancer agents. Electronic supplementary material The online version of this article (doi:10.1186/s40064-015-1352-5) contains supplementary material, which is available to authorized users.
- Published
- 2015
23. Correction: Privileged substructures for anti-sickling activity via cheminformatic analysis
- Author
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Veda Prachayasittikul, Chuleeporn Phanus-umporn, Chanin Nantasenamat, Watshara Shoombuatong, and Nuttapat Anuwongcharoen
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0301 basic medicine ,03 medical and health sciences ,030104 developmental biology ,biology ,Chemistry ,Stereochemistry ,General Chemical Engineering ,biology.protein ,General Chemistry ,Chromatin structure remodeling (RSC) complex - Abstract
Correction for ‘Privileged substructures for anti-sickling activity via cheminformatic analysis’ by Chuleeporn Phanus-umporn et al., RSC Adv., 2018, 8, 5920–5935.
- Published
- 2018
24. Navigating the chemical space of dipeptidyl peptidase-4 inhibitors
- Author
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Virapong Prachayasittikul, Chanin Nantasenamat, Teerawat Monnor, Watshara Shoombuatong, Nuttapat Anuwongcharoen, Veda Prachayasittikul, Supaluk Prachayasittikul, and Napat Songtawee
- Subjects
Quantitative structure–activity relationship ,Stereochemistry ,Protein Conformation ,Surface Properties ,Dipeptidyl Peptidase 4 ,Pharmaceutical Science ,Drug design ,Quantitative Structure-Activity Relationship ,Molecular Docking Simulation ,Polar surface area ,Workflow ,scaffold analysis ,Molecular descriptor ,Drug Discovery ,decision tree ,Humans ,Original Research ,fragment analysis ,Pharmacology ,Dipeptidyl-Peptidase IV Inhibitors ,Principal Component Analysis ,Drug Design, Development and Therapy ,Binding Sites ,Dose-Response Relationship, Drug ,Chemistry ,QSAR ,antidiabetic ,Decision Trees ,Rational design ,Hydrogen Bonding ,molecular docking ,Chemical space ,Molecular Weight ,Docking (molecular) ,Drug Design ,Computer-Aided Design ,rational drug design ,Protein Binding - Abstract
Watshara Shoombuatong,1 Veda Prachayasittikul,1,2 Nuttapat Anuwongcharoen,1 Napat Songtawee,1 Teerawat Monnor,1 Supaluk Prachayasittikul,1 Virapong Prachayasittikul,2 Chanin Nantasenamat1,2 1Center of Data Mining and Biomedical Informatics, 2Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand Abstract: This study represents the first large-scale study on the chemical space of inhibitors of dipeptidyl peptidase-4 (DPP4), which is a potential therapeutic protein target for the treatment of diabetes mellitus. Herein, a large set of 2,937 compounds evaluated for their ability to inhibit DPP4 was compiled from the literature. Molecular descriptors were generated from the geometrically optimized low-energy conformers of these compounds at the semiempirical AM1 level. The origins of DPP4 inhibitory activity were elucidated from computed molecular descriptors that accounted for the unique physicochemical properties inherently present in the active and inactive sets of compounds as defined by their respective half maximal inhibitory concentration values of less than 1 µM and greater than 10 µM, respectively. Decision tree analysis revealed the importance of molecular weight, total energy of a molecule, topological polar surface area, lowest unoccupied molecular orbital, and number of hydrogen-bond donors, which correspond to molecular size, energy, surface polarity, electron acceptors, and hydrogen bond donors, respectively. The prediction model was subjected to rigorous independent testing via three external sets. Scaffold and chemical fragment analysis was also performed on these active and inactive sets of compounds to shed light on the distinguishing features of the functional moieties. Docking of representative active DPP4 inhibitors was also performed to unravel key interacting residues. The results of this study are anticipated to be useful in guiding the rational design of novel and robust DPP4 inhibitors for the treatment of diabetes. Keywords: QSAR, decision tree, scaffold analysis, fragment analysis, antidiabetic, molecular docking, rational drug design
- Published
- 2015
25. On the Origins of Hepatitis C Virus NS5B Polymerase Inhibitory Activity Using Machine Learning Approaches
- Author
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Virapong Prachayasittikul, Apilak Worachartcheewan, Nuttapat Anuwongcharoen, Veda Prachayasittikul, Chanin Nantasenamat, and Watshara Shoombuatong
- Subjects
Hepatitis C virus ,Decision tree ,Biology ,Viral Nonstructural Proteins ,Machine learning ,computer.software_genre ,medicine.disease_cause ,Machine Learning ,chemistry.chemical_compound ,Structure-Activity Relationship ,Drug Discovery ,medicine ,Enzyme Inhibitors ,NS5B ,Artificial neural network ,Molecular Structure ,business.industry ,Rational design ,General Medicine ,Random forest ,High-Throughput Screening Assays ,Support vector machine ,chemistry ,Drug Design ,Artificial intelligence ,business ,computer ,Classifier (UML) - Abstract
Inhibition of non-structural protein 5B (NS5B) represents an attractive strategy for the therapeutic treatment of hepatitis C virus (HCV). In this study, machine learning classifiers such as artificial neural network (ANN), support vector machine (SVM), random forest (RF) and decision tree (DT) analyses were used to classify 970 compounds based on their physicochemical properties, including quantum chemical descriptors, constitutional descriptors, functional groups and molecular properties. Good predictive performance was obtained from all classifiers, providing accuracies ranging from 82.47–89.61% for external validation set. SVM was noted as the best classifier, indicated by its highest accuracy of 89.61%. The analyses were performed on data sets stratified by structural scaffolds (nucleoside and non-nucleoside) and bioactivities (active and inactive properties). In addition, a molecular fragment analysis was performed to investigate molecular substructures corresponding to biological activities. Furthermore, common substructures and potential functional groups governing the activities of active and inactive inhibitors were noted for the benefit of rational design and high-throughput screening towards potential HCV NS5B inhibitors.
- Published
- 2014
26. osFP: a web server for predicting the oligomeric states of fluorescent proteins
- Author
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Saw Simeon, Nuttapat Anuwongcharoen, Jarl E. S. Wikberg, Virapong Prachayasittikul, Likit Preeyanon, Chanin Nantasenamat, and Watshara Shoombuatong
- Subjects
0301 basic medicine ,Web server ,Theoretical computer science ,Source code ,Interface (Java) ,Computer science ,media_common.quotation_subject ,Oligomeric state ,Library and Information Sciences ,computer.software_genre ,GFP ,03 medical and health sciences ,Protein sequencing ,FP ,Green fluorescent protein ,Physical and Theoretical Chemistry ,Peptide sequence ,Data mining ,media_common ,Bioinformatics (Computational Biology) ,030102 biochemistry & molecular biology ,Data curation ,Protein engineering ,Fluorescent protein ,Computer Graphics and Computer-Aided Design ,Computer Science Applications ,030104 developmental biology ,Bioinformatik (beräkningsbiologi) ,Data set (IBM mainframe) ,computer ,Research Article - Abstract
Currently, monomeric fluorescent proteins (FP) are ideal markers for protein tagging. The prediction of oligomeric states is helpful for enhancing live biomedical imaging. Computational prediction of FP oligomeric states can accelerate the effort of protein engineering efforts of creating monomeric FPs. To the best of our knowledge, this study represents the first computational model for predicting and analyzing FP oligomerization directly from the amino acid sequence. After data curation, an exhaustive data set consisting of 397 non-redundant FP oligomeric states was compiled from the literature. Results from benchmarking of the protein descriptors revealed that the model built with amino acid composition descriptors was the top performing model with accuracy, sensitivity and specificity in excess of 80% and MCC greater than 0.6 for all three data subsets (e.g. training, tenfold cross-validation and external sets). The model provided insights on the important residues governing the oligomerization of FP. To maximize the benefit of the generated predictive model, it was implemented as a web server under the R programming environment. osFP affords a user-friendly interface that can be used to predict the oligomeric state of FP using the protein sequence. The advantage of osFP is that it is platform-independent meaning that it can be accessed via a web browser on any operating system and device. osFP is freely accessible at http://codes.bio/osfp/ while the source code and data set is provided on GitHub at https://github.com/chaninn/osFP/ .
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