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Eight lactating cows were fed 4 diets in which dietary crude protein (CP) was increased in steps of approximately 2 percentage units from 11 to 17% of DM by replacing high-moisture corn with soybean meal supplemented with rumen-protected Met to maintain a Lys: Met ratio of 3:1 in metabolizable protein. Trial design was a replicated 4 x 4 Latin square; experimental periods lasted 28 d, with data and sample collection being performed during wk 3 and 4 of each period. Digesta samples were collected from the rumen as well as the omasum to measure metabolite concentrations and ruminal outflow of N fractions using infusion of N-15-enriched ammonia to quantify microbial nonammonia N (NAN) and nonmicrobial NAN. Data were analyzed using the MIXED procedure of SAS (SAS Institute Inc.). There were linear increases in the yields of milk and true protein and concentration of milk urea N, and a linear decrease in N efficiency, with increasing dietary CP. Apparent ruminal and total-tract N digestibility increased linearly with increasing dietary CP, but estimated true total-tract N digestibility was not affected. Apparent digestibility of the other macronutrients was not influenced by diet. Ruminal ammonia, total AA and peptides, and branched-chain VFA also increased linearly with dietary CP. The N-15 enrichment of liquidand particle-associated microbes linearly declined with increasing dietary CP due to decreasing 15N enrichment of the ammonia pool. Although no effect of dietary CP on nonmicrobial NAN flow was detected, total NAN flow increased linearly from 525 g/d at 11% CP to 637 g/d at 17% CP due to the linear increase in microbial NAN flow from 406 g/d at 11% CP to 482 g/d at 17% CP. Under the conditions of this study, when dietary CP was increased by adding soybean meal supplemented with rumen-protected Met, improved milk and protein yields were driven not by RUP supply but by increased ruminal outflow of microbial protein.

The objective of this study was to evaluate the effects of 0.5% urea and 0.5% urea plus 4% molasses (on weight basis) addition to sorghum silages ensiled using 4 sorghum varieties (Grass II, Grazer, Gozde, and P-988) harvested at the milk stage. Plant materials were ensiled in 1-kg jars and the chemical composition, pH, organic acids, in vitro digestibility (IVDMD) and digestible dry matter yield (DDMY) of the silages were determined. Addition of urea and urea plus molasses to silages increased the crude protein ratio significantly (P < 0.01). However, the addition of urea plus molasses significantly decreased the neutral detergent fiber and acid detergent fiber contents of the silages. Additives significantly increased the pH values of the silages. The addition of urea and urea plus molasses to silages generally did not affect acetic or propionic acid content; however, the butyric acid content of the urea and urea plus molasses groups was higher than that of the control groups. IVDMD of all sorghum varieties decreased with the addition of urea and urea plus molasses. Additives did not affect DDMY. In conclusion, the addition of 0.5% urea and 0.5% urea plus 4% molasses to sorghum silages, harvested at the milk stage, improved the CP content of the silages, but they had no positive effect on silage quality, IVDMD or DDMY.

The aim of the study was to evaluate the in situ degradation kinetics of some commonly used protein sources (sunflower meal, cottonseed meal, and soybean meal). Three ruminally fistulated rams were utilized in the study. They were fed ground alfalfa and a mixture of concentrate (60% alfalfa and 40% mixture) at maintenance level. The Dacron bags used in the study had a 45 p 2, pore size and internal dimensions of 15 x 7 cm. Feed samples were ground to pass through a 2 mm screen. Samples were incubated in the rumen of rams for 0, 2, 4, 8, 12, 24 and 48 h. Each feed sample was incubated in the rumen of 3 rams in duplicate for each incubation time. Approximately 3.5 g of samples were placed in each Dacron bag.

Direct-fed microbes (DFM) include bacteria, yeast or combination of them which are added into diet. They are commonly used to enhance growth rate, feed efficiency and egg yield in poultry rations. Yeast cultures (YC) contain viable yeast, metabolites, and the media on which the yeast cells are grown (Bradley and Savage, 1995). The addition of live yeast cultures to chick diets have been used to improve egg production. (Nahashon et al., 1996), animal performance (Onifade et al., 1998; Purushotha-man and Natanam, 1999), enhance feed-efficiency (Kahraman et al., 1996), improve, fatty acid digestibility, and to reduce abdominal fat content (Lacroix et al., 1991). In contrast, Elbert et al. (1987), Brake (1991) and Lacroix et al. (loc. cit.) have reported no effect of dietary YC in balanced chicken diets. The aim of this study was to assess egg yield and quality in hens fed different levels of yeast culture at last period of laying.

The aim of this study was to determine the effects of urea or urea plus molasses addition into corn harvested at the milk stage on the silage quality and in vitro digestibility of silage. To achieve this objective, four different corn varieties (Rx-947, 33-94, Frassino and Arifiye) harvested at the milk stage were utilized. Silage samples were added 0.5% urea or 0.5% urea plus 4% molasses and ensiled in mini-silos (I L in volume).

The objective of this study was to determine sorghum varieties that are suitable for north Anatolian conditions and possess high nutrient yields. Eight grain sorghum varieties, namely, Ogretmenoglu, Akdari, Aldari, Beydari, Ramada, A-298, DK-64 and KS-397, were utilized as plant material in the experiment. When the grains were mature, they were harvested first, and then residues were harvested by hand. Samples were analyzed for dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), and in vitro DM digestibility. Digestible DM yields were also calculated. The DM content of residues ranged from 26.64% to 35.36% (P < 0.05). Concentrations of OM in residues were significantly lower (P < 0.05) in Ramada and Aldari compared with the other varieties. While NDF and ADF concentrations in residues were highest in Beydari, they were lowest in DK-64 (P < 0.05). Residues of DK-64 and Aldari had the highest in vitro digestibilities, DM yields and digestible DM yields (P < 0.05). Ogretmentoglu grain had the highest CP, whereas Beydari had the lowest in vitro digestibility (P < 0.05). Ramada grain had the highest DM and digestible DM yields (P < 0.05). Total (forage + grain) DM yield, digestible DM yield and CP yield were significantly higher in Ramada and A-298 compared with the other varieties (P < 0.05). It can be concluded that, of the sorghum varieties grown, Ramada had the highest yield and adaptation for north Anatoilan conditions, followed by A-298, based on digestible DM and CP yields, respectively.

The objective of this study-was to determine the effects of maturity on the range quality and weight gain of male and female lambs, and the time at which supplementation is needed.

The objective of this study was to investigate the effects of baker's yeast as a protein source instead of soybean meal in dairy cow diets. Four dairy cows were utilized in a 4 x 4 Latin Square experiment. Diets included 0.0% (Control Diet), 6.6% (Diet 1), 13.2% (Diet 2), and 19.8% (Diet 3) baker's yeast containing 3.56 x 10(8) CFU/g. Forty percent of the dry matter intake of dairy cows was supplied from concentrate, and forage was offered ad libitum. The dry matter intake, milk yield, and composition of milk were similar. While the rumen NH3-N level was significantly low (p < 0.05) 3 h post-feeding, acetic acid concentration was significantly high (p < 0.05) 12 h post-feeding in cows that consumed diets containing yeast. Propionic acid concentrations were lower (p < 0.01) 3 h post-feeding in cows that consumed Diets 2 and 3 compared with those that consumed the other diets. While serum triglyceride levels were significantly higher (p < 0.05) in cows that consumed diets containing baker's yeast than in those that consumed the control diet, serum protein, urea, glucose, calcium, phosphorus, gamma glutamyl transferase and aspartate aminotransferase were similar in cows that consumed different diets. It was concluded from this study that there were some positive effects, such as low rumen NH3-N level and high acetic acid concentration, in cows that consumed diets containing yeast, and thus, baker's yeast can be utilized in dairy cow diets as a protein source.

The objective of this experiment was to evaluate ensiling methods for more efficient and long lasting preservation of sugar beet pulp which is an energy rich cheap feedstuff For this reason during ensiling the dry matter of sugar beet pulp was increased to 20% by adding wheat straw or grass hay On the other hand 1 % urea + 5% beet molasses (SBPSM) and 0% urea + 5% beet molasses (SBPHM) were added to these silages containing wheat straw or grass hay respectively Two kinds of beet pulp silages corn silage and grass hay were utilized in lambs in this experiment In the digestion trial organic matter digestibilities were 60 39 58 99 55 80 and 60 03% (P

The objective of this experiment was to evaluate the ensiling methods for the more efficient and long lasting preservation of sugar beet pulp which is rich in energy and is a source of inexpensive feedstuff The ruminal degradabilities of these silages were determined