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COVID-19 pandemic had a negative impact on adolescents’ psychological health. Two national studies were conducted. The DGEEC study included 9 to 18-year-old pupils. The HBSC study (2 waves) included pupils from the 6th, 8th, 10th, 12th grades. For each study, two groups were established based on life satisfaction scores and on psychological symptoms scores, producing four groups. In both studies, the group with high life satisfaction and reduced psychological symptoms had more boys and younger pupils. The group with low life satisfaction and pronounced psychological symptoms comprised more girls and older pupils. In the DGEEC Study, pupils with low life satisfaction and pronounced psychological symptoms were more likely to exhibit other unwellness signs. In the HBSC study, from 2018 to 2022 (with the COVID-19 in between), psychological symptoms increased significantly and life satisfaction decreased, exacerbating inequities. This model allowed to examine the results of DGEEC and HBSC studies under a new perspective comprising four scenarios, offering an innovative viewpoint on adolescents’ psychological health. Results also suggest a potential buffering effect of being physically active, adequate sleep, and adequate screen time. This is a powerful message for health and education professionals and policymakers regarding the relevance of health education and promotion.

© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)., During and in the aftermath of the COVID-19 pandemic, several works reflected on young people’s physical and psychological health. The Dual Factor Model, which we refer to as the quadripartite model, is useful for understanding children’s and adolescents’ psychological health and differentiating them regarding their attitude toward the effects of the COVID-19 pandemic. In this investigation, students from the fifth to twelfth year of schooling enrolled in the DGEEC study “Psychological Health and Wellbeing in Portuguese schools” were considered. Four groups were created based on life satisfaction (low or high) and psychological distress (with or without symptoms). The study included 4444 students (M = 13.39 years ± 2.41), of whom 47.8% were male. Of the participants, 27.2% were in the second cycle of primary education, and 72.8% were in lower and upper secondary education. Differences in gender and education level (as a proxy for age) were observed. Additionally, when considering students’ perceptions of changes in their lives following the COVID-19 pandemic (stayed the same, became worse, became better), these three groups were compared concerning personal and contextual variables, revealing significant differences at both the individual and contextual levels. Finally, the study discusses the influence of education and health professionals and the need for friendly public policies., DGEEC study was funded by Directorate General of Education and Science Statistics.

BackgroundThoroughbred racehorse performance is largely influenced by a major quantitative trait locus at the myostatin (MSTN) gene which determines aptitude for certain race distances due to a promoter region insertion mutation influencing functional phenotypes in skeletal muscle. To develop an in vitro system for functional experiments we established three novel equine skeletal muscle cell lines reflecting the variation in phenotype associated with MSTN genotype (CC/II, CT/IN and TT/NN for SNP g.66493737C>T/SINE insertion 227 bp polymorphism). Primary equine skeletal muscle myoblasts, isolated from Thoroughbred horse gluteus medius, were conditionally immortalised and evaluated to determine whether cell phenotype and metabolic function were comparable to functional characteristics previously reported for ex vivo skeletal muscle isolated from Thoroughbred horses with each genotype.ResultsPrimary myoblasts conditionally immortalized with the temperature sensitive SV40TtsA58 lentivirus vector successfully proliferated and could revert to their primary cell phenotype and differentiate into multinucleated myotubes. Skeletal muscle fibre type, MSTN gene expression, mitochondrial abundance, and mitochondrial function of the three MSTN genotype cell lines, were consistent with equivalent characterisation of ex vivo skeletal muscle samples with these genotypes. Furthermore, addition of coenzyme Q10 (CoQ10) to the cell lines improved mitochondrial function, an observation consistent with ex vivo skeletal muscle samples with these genotypes following supplementation with CoQ10 in the diet. ConclusionsThe observation that the phenotypic characteristics and metabolic function of the cells lines are equivalent to ex vivo skeletal muscle indicates that this in vitro system will enable efficient and cost-effective analyses of equine skeletal muscle for a range of different applications including understanding metabolic function, testing of nutritional supplements, drug test development and gene doping test development. In the multi-billion-euro international Thoroughbred horse industry research advances in the biological function of skeletal muscle are likely to have considerable impact. Furthermore, this novel genotype-specific system may be adapted and applied to human biomedicine to improve understanding of the effects of myostatin in human physiology and medicine.

The stem cell niche at the perivascular space in human tissue plays a pivotal role in dictating the overall fate of stem cells within it. Mesenchymal stem cells (MSCs) in particular, experience influential microenvironmental conditions, which induce specific metabolic profiles that affect processes of cell differentiation and dysregulation of the immunomodulatory function. Reports focusing specifically on the metabolic status of MSCs under the effect of pathophysiological stimuli - in terms of flow velocities, shear stresses or oxygen tension - do not model heterogeneous gradients, highlighting the need for more advanced models reproducing the metabolic niche. Organ-on-a-chip technology offers the most advanced tools for stem cell niche modelling thus allowing for controlled dynamic culture conditions while profiling tuneable oxygen tension gradients. However, current systems for live cell detection of metabolic activity inside microfluidic devices require the integration of microsensors. The presence of such microsensors poses the potential to alter microfluidics and their resolution does not enable intracellular measurements but rather a global representation concerning cellular metabolism. Here, we present a metabolic toolbox coupling a miniaturised in vitro system for human-MSCs dynamic culture, which mimics microenvironmental conditions of the perivascular niche, with high-resolution imaging of cell metabolism. Using fluorescence lifetime imaging microscopy (FLIM) we monitor the spatial metabolic machinery and correlate it with experimentally validated intracellular oxygen concentration after designing the oxygen tension decay along the fluidic chamber by in silico models prediction. Our platform allows the metabolic regulation of MSCs, mimicking the physiological niche in space and time, and its real-time monitoring representing a functional tool for modelling perivascular niches, relevant diseases and metabolic-related uptake of pharmaceuticals.

Despite previous attempts, the Early Neolithic of Portugal was poorly understood until the latter part of the twentieth century. It is only when Guilaine and Ferreira (1970) re-analysed pottery assemblages kept in museums across the country and compared them with parallels elsewhere in Iberia and southern France that they were able to distinguish between an earlier Cardial phase and a more recent stage, named ‘Furninha horizon’ after an important burial cave excavated in 1880. Essentially, most Portuguese prehistorians still use this scheme today. Though some have argued in favour of pre-Cardial phases, either of African or Andalusian origin (e.g. Silva & Soares, 1981) or represented by impressa-type ceramics of Italic origin (e.g. Guilaine, 2018), these hypotheses are still lacking sound empirical support (Carvalho, 2020). It should, however, be noted that these hypotheses are still sometimes taken up in discussions of new finds. This is the case in a recently-published ovoid vase, with a flat base and impressed decoration, retrieved from so-called ‘hearth 8’ at the open-air site of Vale Pincel (coastal Alentejo), which was dated to c. 5650 cal BC. As this predates the oldest Cardial in Portuguese territory and is not a Cardial vessel, the author claims that this ‘ceramic decoration is part of the pre-Cardial impressed world’ (Soares, 2020: 311–2 and fig. 4). info:eu-repo/semantics/publishedVersion

The paper presents the development of an inclusive serious multiplayer game with Morse code as its theme, as well as describing methodologies and architectures for developing a serious game through specific concepts for the deaf (sign language) and blind (audio description). The preceding case has three game modes: classic one versus one, a cooperative mode between two players and a four-player game mode all. To validate this serious game performance and quality, a Quantitative Evaluation Framework was used. In that context, the final product has three dimensions: pedagogical, ergonomic, and technical. From the obtained data, it was possible to conclude that this game was proof that using accessibility guidelines during software development helps to promote not only an efficient integration of excluded communities, but also social inclusion and equity between players.

Inflammatory arthritis is a chronic systemic autoimmune disease of unknown etiology, which affects the joints. If untreated, these diseases can have a detrimental effect on the patient's quality of life, leading to disabilities, and therefore, exhibit a significant socioeconomic impact and burden. While studies of immune cell populations in arthritis patient's peripheral blood have been informative regarding potential immune cell dysfunction and possible patient stratification, there are considerable limitations in identifying the early events that lead to synovial inflammation. The joint, as the site of inflammation and the local microenvironment, exhibit unique characteristics that contribute to disease pathogenesis. Understanding the contribution of immune and stromal cell interactions within the inflamed joint has been met with several technical challenges. Additionally, the limited availability of synovial tissue biopsies is a key incentive for the utilization of high-throughput techniques in order to maximize information gain. This review aims to provide an overview of key methods and novel techniques that are used in the handling, processing and analysis of synovial tissue biopsies and the potential synergy between these techniques. Herein, we describe the utilization of high dimensionality flow cytometric analysis, single cell RNA sequencing, ex vivo functional assays and non-intrusive metabolic characterization of synovial cells on a single cell level based on fluorescent lifetime imaging microscopy. Additionally, we recommend important points of consideration regarding the effect of different storage and handling techniques on downstream analysis of synovial tissue samples. The introduction of new powerful techniques in the study of synovial tissue inflammation, brings new challenges but importantly, significant opportunities. Implementation of novel approaches will accelerate our path toward understanding of the mechanisms involved in the pathogenesis of inflammatory arthritis and lead to the identification of new avenues of therapeutic intervention.

ObjectivesImmune and stromal cell communication is central in the pathogenesis of rheumatoid arthritis (RA) and psoriatic arthritis (PsA), however, the nature of these interactions in the synovial pathology of the two pathotypes can differ. Identifying immune-stromal cell crosstalk at the site of inflammation in RA and PsA is challenging. This study creates the first global transcriptomic analysis of the RA and PsA inflamed joint and investigates immune-stromal cell interactions in the pathogenesis of synovial inflammation.MethodsSingle cell transcriptomic profiling of 178 000 synovial tissue cells from five patients with PsA and four patients with RA, importantly, without prior sorting of immune and stromal cells. This approach enabled the transcriptomic analysis of the intact synovial tissue and identification of immune and stromal cell interactions. State of the art data integration and annotation techniques identified and characterised 18 stromal and 14 immune cell clusters.ResultsGlobal transcriptomic analysis of synovial cell subsets identifies actively proliferating synovial T cells and indicates that due to differential λ and κ immunoglobulin light chain usage, synovial plasma cells are potentially not derived from the local memory B cell pool. Importantly, we report distinct fibroblast and endothelial cell transcriptomes indicating abundant subpopulations in RA and PsA characterised by differential transcription factor usage. Using receptor–ligand interactions and downstream target characterisation, we identify RA-specific synovial T cell-derived transforming growth factor (TGF)-β and macrophage interleukin (IL)-1β synergy in driving the transcriptional profile of FAPα+THY1+invasive synovial fibroblasts, expanded in RA compared with PsA. In vitro characterisation of patient with RA synovial fibroblasts showed metabolic switch to glycolysis, increased adhesion intercellular adhesion molecules 1 expression and IL-6 secretion in response to combined TGF-β and IL-1β treatment. Disrupting specific immune and stromal cell interactions offers novel opportunities for targeted therapeutic intervention in RA and PsA.

ObjectivesThis study investigates pathogenic and protective polyfunctional T-cell responses in patient with rheumatoid arthritis (RA), individuals at risk (IAR) and healthy control (HC) synovial-tissue biopsies and identifies the presence of a novel population of pathogenic polyfunctional T-cells that are enriched in the RA joint prior to the development of clinical inflammation.MethodsPathway enrichment analysis of previously obtained RNAseq data of synovial biopsies from RA (n=118), IAR (n=20) and HC (n=44) was performed. Single-cell synovial tissue suspensions from RA (n=10), IAR (n=7) and HC (n=7) and paired peripheral blood mononuclear cells (PBMC) were stimulated in vitro and polyfunctional synovial T-cell subsets examined by flow cytometric analysis, simplified presentation of incredibly complex evaluations (SPICE) and FlowSom clustering. Flow-imaging was utilised to confirm specific T-cell cluster identification. Fluorescent lifetime imaging microscopy (FLIM) was used to visualise metabolic status of sorted T-cell populations.ResultsIncreased plasticity of Tfh cells and CD4 T-cell polyfunctionality with enriched memory Treg cell responses was demonstrated in RA patient synovial tissue. Synovial-tissue RNAseq analysis reveals that enrichment in T-cell activation and differentiation pathways pre-dates the onset of RA. Switch from potentially protective IL-4 and granulocyte macrophage colony stimulating factor (GMCSF) dominated polyfunctional CD4 T-cell responses towards pathogenic polyfunctionality is evident in patient with IAR and RA synovial tissue. Cluster analysis reveals the accumulation of highly polyfunctional CD4+ CD8dim T-cells in IAR and RA but not HC synovial tissue. CD4+ CD8dim T-cells show increased utilisation of oxidative phosphorylation, a characteristic of metabolically primed memory T-cells. Frequency of synovial CD4+ CD8dim T-cells correlates with RA disease activity.ConclusionSwitch from potentially protective to pathogenic T-cell polyfunctionality pre-dates the onset of clinical inflammation and constitutes an opportunity for therapeutic intervention in RA.

While autoantibodies are used in the diagnosis of rheumatoid arthritis (RA), the function of B cells in the inflamed joint remains elusive. Extensive flow cytometric characterization and SPICE algorithm analyses of single-cell synovial tissue from patients with RA revealed the accumulation of switched and double-negative memory programmed death-1 receptor–expressing (PD-1–expressing) B cells at the site of inflammation. Accumulation of memory B cells was mediated by CXCR3, evident by the observed increase in CXCR3-expressing synovial B cells compared with the periphery, differential regulation by key synovial cytokines, and restricted B cell invasion demonstrated in response to CXCR3 blockade. Notably, under 3% O2 hypoxic conditions that mimic the joint microenvironment, RA B cells maintained marked expression of MMP-9, TNF, and IL-6, with PD-1+ B cells demonstrating higher expression of CXCR3, CD80, CD86, IL-1β, and GM-CSF than their PD-1– counterparts. Finally, following functional analysis and flow cell sorting of RA PD-1+ versus PD-1– B cells, we demonstrate, using RNA-Seq and emerging fluorescence lifetime imaging microscopy of cellular NAD, a significant shift in metabolism of RA PD-1+ B cells toward glycolysis, associated with an increased transcriptional signature of key cytokines and chemokines that are strongly implicated in RA pathogenesis. Our data support the targeting of pathogenic PD-1+ B cells in RA as a focused, novel therapeutic option., A pathogenic glycolytic B cell population at the site of inflammation in patients with Rheumatoid Arthritis associates with disease severity.

The stem cell niche at the perivascular space in human tissue plays a pivotal role in dictating the overall fate of stem cells within it. Mesenchymal stem cells (MSCs), in particular, experience influential microenvironmental conditions, which induce specific metabolic profiles that affect processes such as cell differentiation and dysregulation of the immunomodulatory funtion. Reports focusing specifically on the metabolic status of MSCs under the effect of pathophysiological stimuli - in terms of flow velocities, shear stresses or oxygen tension – do not model heterogenous gradients, highlighting the need of more advanced models reproducing the metabolic niche. Organ-on-a-chip technology offers the most advanced tools for stem cell niche modelling thus allowing for controlled dynamic culture conditions while profiling tunable oxygen tension gradients. However, current systems for live cell detection of metabolic activity inside microfluidic devices require the integration of microsensors that allow for extracellular measurments only, giving innacurate and indirect information about the metabolic state of cells. Here, we present a metabolic toolbox coupling a miniatuirzed in vitro system for human-MSCs dynamic culture, that mimics microenvironmental conditions of the perivascular niche, with high-resolution imaging of intracellular metabolism. Using Fluorescence Lifetime Imaging Microscopy (FLIM) we monitor the spatial metabolic machinery and correlate it with experimentally validated intracellular oxygen concentration after designing the oxygen tension decay along the fluidic chamber by in silico models prediction. Our platform allows for the subjection of a metabolic profile to MSCs, mimicking the physiological niche in space and time, and its real-time monitoring representing a functional tool for modelling perivascular niches, relevant diseases and metabolic-related uptake of pharmaceuticals.

The paper presents the development of an inclusive serious multiplayer game with Morse code as its theme, as well as describing methodologies and architectures for developing a serious game through specific concepts for the deaf (sign language) and blind (audio description). The preceding case has three game modes: classic one versus one, a cooperative mode between two players and a four-player game mode all. In order to validate this serious game performance and quality, the final product has three dimensions: pedagogical, ergonomic and technical. From the obtained data, it was possible to conclude that this game was proof that using accessibility guidelines during software development helps to promote not only an efficient integration of excluded communities, but also social inclusion and equity between players.

Stem cells and the niche in which they reside feature a complex microenvironment with tightly regulated homeostasis, cell-cell interactions and dynamic regulation of metabolism. A significant number of organoid models has been described over the last decade, yet few methodologies can enable single cell level resolution analysis of the stem cell niche metabolic demands, in real-time and without perturbing integrity. Here, we studied the redox metabolism of Lgr5-GFP intestinal organoids by two emerging microscopy approaches based on luminescence lifetime measurement – fluorescence-based FLIM for NAD(P)H, and phosphorescence-based PLIM for real-time oxygenation. We found that exposure of stem (Lgr5-GFP) and differentiated (no GFP) cells to high and low glucose concentrations resulted in measurable shifts in oxygenation and redox status. NAD(P)H-FLIM and O2-PLIM both indicated that at high ‘basal’ glucose conditions, Lgr5-GFP cells had lower activity of oxidative phosphorylation when compared with cells lacking Lgr5. However, when exposed to low (0.5 mM) glucose, stem cells utilized oxidative metabolism more dynamically than non-stem cells. The high heterogeneity of complex 3D architecture and energy production pathways of Lgr5-GFP organoids were also confirmed by the extracellular flux (XF) analysis. Our data reveals that combined analysis of NAD(P)H-FLIM and organoid oxygenation by PLIM represents promising approach for studying stem cell niche metabolism in a live readout., Highlights • Adult stem cell-derived intestinal organoids have strong, heterogeneous and poorly understood oxidative metabolism. • We compared O2-PLIM, NAD(P)H-FLIM and extracellular flux analysis to study cell metabolism in the intestinal organoids. • Oxygenation of stem and differentiated cells in organoids correlates with their reliance on the energy production pathways. • Lgr5-GFP+ cells quickly up- and down-regulate their OxPhos/glycolysis in response to glucose and pyruvate availability.

Serious games have a great potential to help the population develop new skills or improve previously existing ones. However, part of the population does not have the means to play these games. Therefore, this article aims to discuss the use of serious games for deaf and blind people, as well as show the development of serious games inclusive, presenting specific concepts for each target audience. We used the cognitive model proposed by Mayer (2005) to describe some fundamental principles behind multimedia learning, defined from his theory and based on the evidences that are essential for the elaboration of serious games for deaf and blind people. We hope that the games developed, and guidelines provided will help game designers and game developers to achieve successful implementation in inclusive games.

Background:Synovial tissue macrophages are an exquisitely plastic pool of innate cells that play a key role in RA disease progression. However, the precise nature, diversity, and function of macrophage subsets within the inflamed joint remains unexplored.Objectives:Therefore, the aims of this study are to phenotypically, transcriptionally and functionally characterise synovial tissue macrophages residing within the inflamed joint.Methods:Rheumatoid Arthritis, Psoriatic Arthritis, Osteoarthritis and healthy control synovial-tissue biopsies and synovial-fluid mononuclear cells were analysed using the following panel (CD40,-CD45,-CD64,-CD68,-CD163,-CD206,-CD253,-CCR4,-CCR7,-CXCR1,-CXCR3). CD206+CD163+ and CD206-CD163- macrophages were sorted from RA synovial-tissue by FACSAria sorter; RNAseq and FLIM analysis, autologous T-cell co-culture and heathy fibroblast experiments performed. Cytokine expression was measured by MSD immunoassay.Results:RA synovial tissue and fluid macrophages display markers typical of both M1 (CD40+CD253+) and M2 (CD206+CD163+) macrophages with a spectrum of macrophage activation states identified. Within this spectrum, significant enrichment of dominant CD206+CD163+ macrophage-subtype is present in synovial tissue versus fluid (pConclusion:This data identifies for the first-time enrichment of a previously undescribed dysfunctional dominant and transcriptionally distinct macrophage subtype in RA synovial tissue. Taken together, this data provides a greater understanding of the critical role tissue-resident macrophages play in perpetuating inflammation in RA. Further investigation of the molecular patterns and cues that shape specific synovial macrophage subsets may provide opportunities to reinstate RA joint homeostasis.Disclosure of Interests:Megan Hanlon: None declared, Mary Canavan: None declared, Qingxuan Song Employee of: Janssen Research & Development, Nuno Neto: None declared, Phil Gallagher: None declared, Ronan Mullan: None declared, Conor Hurson: None declared, Michael Monaghan: None declared, Sunil Nagpal Employee of: Janssen Research & Development, Douglas Veale Speakers bureau: Abbvie, Janssen, Novartis, MSD, Pfizer, UCB, Consultant of: Abbvie, Janssen, Novartis, MSD, Pfizer, UCB, Grant/research support from: Janssen, Abbvie, Pfizer, UCB, Ursula Fearon Speakers bureau: Abbvie, Grant/research support from: Janssen, Abbvie, Pfizer, UCB

This study reports that activation of SARM1 causes destruction of NAD pools in photoreceptor cells of the retina and identifies a role for SARM1-dependent photoreceptor cell death in retinal degeneration., Retinal degeneration is the leading cause of incurable blindness worldwide and is characterised by progressive loss of light-sensing photoreceptors in the neural retina. SARM1 is known for its role in axonal degeneration, but a role for SARM1 in photoreceptor cell degeneration has not been reported. SARM1 is known to mediate neuronal cell degeneration through depletion of essential metabolite NAD and induction of energy crisis. Here, we demonstrate that SARM1 is expressed in photoreceptors, and using retinal tissue explant, we confirm that activation of SARM1 causes destruction of NAD pools in the photoreceptor layer. Through generation of rho−/−sarm1−/− double knockout mice, we demonstrate that genetic deletion of SARM1 promotes both rod and cone photoreceptor cell survival in the rhodopsin knockout (rho−/−) mouse model of photoreceptor degeneration. Finally, we demonstrate that SARM1 deficiency preserves cone visual function in the surviving photoreceptors when assayed by electroretinography. Overall, our data indicate that endogenous SARM1 has the capacity to consume NAD in photoreceptor cells and identifies a previously unappreciated role for SARM1-dependent cell death in photoreceptor cell degeneration.

Recent archaeological work in northeast Portugal has led to the identification of two Upper Paleolithic sites in an area traditionally considered lacking in Old Stone Age evidence: Tras-os-Montes. The new research reveals the existence of the first Upper Paleolithic sequence to the north of the Portuguese Douro (Duero). The Middle Solutrean site of Foz do Medal on the left bank of the Sabor River adds relevant data to the discussion about human adaptations during the Last Glacial Maximum and the gap in knowledge regarding the use of interior territories in the Iberian Peninsula.

A strategy assisted by an inorganic template was developed to promote the organized self-assembly of meso-(tetrakis)-(p-sulfonatophenyl)porphyrin (TPPS) on pH-sensitive core-shell polyelectrolyte microcapsules (PECs) of poly(styrenesulfonate) (PSS) and poly(allylamine hydrochloride) (PAH). A key feature of this strategy is the use of template CaCO3 microparticles as a nucleation site endorsing inside-outside directional growth of porphyrin aggregates. Using this approach, TPPS self-assembly in positively charged PECs with CaCO3 (PAH/PSS)2PAH as a sequence of layers was successfully achieved using mild pH conditions (pH 3). Evidence for porphyrin aggregation was obtained by UV-vis with the characteristic absorption bands in PECs functionalized with porphyrins. Fluorescence lifetime imaging microscopy (FLIM) of the polyelectrolyte core-shell confirmed the presence of radially distributed needlelike structures sticking out from polyelectrolyte shells. Microscopic images also revealed a sequential process (adsorption, redistribution, and aggregation) for the directional growth (inside/outside) of TPPS aggregates, which highlights the importance of the core in the aggregation induction. Removing the CaCO3 core alters the porphyrin interaction in the PEC environment, and aggregate growth is no longer favored.

Aiming the construction of a new hotel (Hotel Cais de Santarém) using the previous basement of the large storageknown as Sommer, located in Rua Cais de Santarém, nºs 40 up to 64, and limited by Rua de São João daPraça northwards and by Travessa de São João da Praça eastwards, in the city of Lisbon, a large amount ofarchaeological contexts were uncovered that uniquely illustrate the stratification of the city of Lisbon betweenthe Iron Age and the first half of the 20th century. Concerning Roman urban contexts was found a domus withrooms well preserved with painted walls plaster and a pavement covered with mosaic. In this paper the authorswill present the monument and further discussions will take place on excavation levels, iconographic and architecturalfeatures., Lizbon kentinde, Rua Cais de Santarém’de, 40-64 numaralı parseller arasında, kuzeyde Rua de São João daPraça ve doğuda Travessa de São João da Praça ile sınırlanan alanda, Sommer olarak bilinen eski ve oldukçageniş bir deponun temelleri üzerine inşa edilmesi planlanan Cais de Santarém isimli yeni bir otel çalışmalarıesnasında, Demir Çağı’ndan başlayarak 20. yüzyılın ilk yarısına kadar uzanan geniş bir zaman dilimini kapsayanve Lizbon kentinin zaman içerisindeki gelişimini benzersiz bir şekilde gözler önüne seren arkeolojik tabakalanmatespit edilmiştir. Roma kent konseptine ilişkin olarak iyi korunmuş odalara, boyalı duvar plasterlerineve mozaik döşemesine sahip bir domus tespit edilmiştir. Bu çalışmada, söz konusu yapı tanıtılacak olup, kazıçalışmaları, tabakalanma, mimari ve ikonografik özellikler tartışılacaktır.

We are currently in the process of making a series of interconnected multimedia applications, in order to value and enliven the monuments of Sintra, Portugal - classified by Unesco as World Heritage site in 1995. This national project, Fala Comigo (Talk2Me), aspires to present new contents, not only to scholars but also to an unlimited number of visitors, and thus achieve a social, cultural, educational and civic mission. As an interdisciplinary project, the historians generate the content, while the engineers and technicians design content-driven multimedia applications. In this phase, our case study is the Monserrate Palace, a revivalist building of the 19th century. These applications profoundly rely on a balance between renewed heritage contents, a rigorous scientific approach and stunning designs. We encounter innovative multimodal ways of visitor-application interaction, mainly with the addition of virtual agents that works as guide assistants with educational purposes. INOV, IST, UTL, Lisbon, Portugal, Art History Institute, UL, Lisbon, Portugal.

O presente artigo pretende dar a conhecer dois exemplos de aplicação dos métodos da arqueologia da arquitectura em contexto urbano, nomeadamente um conjunto edificado do centro histórico de Lagos e um prédio de habitação na cidade de Lisboa. Os exemplos dados foram alvo de intervenções arqueológicas com objectivos diferentes devido ao contexto de obra a que estavam sujeitos: o primeiro, condenado a demolição total para construção de um novo edifício; o segundo sofrendo uma obra de recuperação, afectando os elementos estruturais apenas ao nível dos revestimentos. Para além da interpretação dos dados obtidos para cada um dos edifícios, pretende-se apresentar as condicionantes que determinaram a metodologia a empregar em cada caso.

Mestrado em Ciências Empresariais O presente trabalho investiga as exportações portuguesas para o México. Após uma análise genérica ao ambiente de negócios no México e às relações comerciais entre os dois países, foi realizado um estudo quantitativo que reuniu questionários enviados por email a empresas que exportam para este país. Os resultados recolhem informações acerca da entrada e do conhecimento do mercado, da forma de exportação adoptada, do relacionamento com os clientes e das dificuldades e expectativas em relação ao mercado. This paper is an investigation work on Portuguese exports to Mexico. After a generic overview on México business environment and on economic relationships between both countries, a quantitative study was done using an email survey among those Portuguese exporting firms. The findings of this investigation collect information about knowledge and market choice, export modes, customer relationships, difficulties and market expectations of doing business.