13 results on '"Nunez MG"'
Search Results
2. A dominant function of LynB kinase in preventing autoimmunity.
- Author
-
Brian BF 4th, Sauer ML, Greene JT, Senevirathne SE, Lindstedt AJ, Funk OL, Ruis BL, Ramirez LA, Auger JL, Swanson WL, Nunez MG, Moriarity BS, Lowell CA, Binstadt BA, and Freedman TS
- Abstract
Here, we report that the LynB splice variant of the Src-family kinase Lyn exerts a dominant immunosuppressive function in vivo, whereas the LynA isoform is uniquely required to restrain autoimmunity in female mice. We used CRISPR-Cas9 gene editing to constrain lyn splicing and expression, generating single-isoform LynA knockout (LynA
KO ) or LynBKO mice. Autoimmune disease in total LynKO mice is characterized by production of antinuclear antibodies, glomerulonephritis, impaired B cell development, and overabundance of activated B cells and proinflammatory myeloid cells. Expression of LynA or LynB alone uncoupled the developmental phenotype from the autoimmune disease: B cell transitional populations were restored, but myeloid cells and differentiated B cells were dysregulated. These changes were isoform-specific, sexually dimorphic, and distinct from the complete LynKO . Despite the apparent differences in disease etiology and penetrance, loss of either LynA or LynB had the potential to induce severe autoimmune disease with parallels to human systemic lupus erythematosus (SLE).- Published
- 2022
- Full Text
- View/download PDF
3. Community Interventions to Promote Mental Health and Social Equity.
- Author
-
Castillo EG, Ijadi-Maghsoodi R, Shadravan S, Moore E, Mensah MO 3rd, Docherty M, Aguilera Nunez MG, Barcelo N, Goodsmith N, Halpin LE, Morton I, Mango J, Montero AE, Koushkaki SR, Bromley E, Chung B, Jones F, Gabrielian S, Gelberg L, Greenberg JM, Kalofonos I, Kataoka SH, Miranda J, Pincus HA, Zima BT, and Wells KB
- Abstract
(Reprinted with permission from Current Psychiatry Reports (2020) 21: 35)., (Copyright © 2020 by the American Psychiatric Association.)
- Published
- 2020
- Full Text
- View/download PDF
4. Unique-region phosphorylation targets LynA for rapid degradation, tuning its expression and signaling in myeloid cells.
- Author
-
Brian BF 4th, Jolicoeur AS, Guerrero CR, Nunez MG, Sychev ZE, Hegre SA, Sætrom P, Habib N, Drake JM, Schwertfeger KL, and Freedman TS
- Subjects
- Animals, Humans, Jurkat Cells, Mice, Knockout, Phosphorylation, Proteolysis, Proto-Oncogene Proteins c-cbl genetics, Ubiquitin metabolism, src-Family Kinases genetics, Macrophages metabolism, Mast Cells metabolism, Myeloid Cells metabolism, Proto-Oncogene Proteins c-cbl metabolism, src-Family Kinases metabolism
- Abstract
The activity of Src-family kinases (SFKs), which phosphorylate immunoreceptor tyrosine-based activation motifs (ITAMs), is a critical factor regulating myeloid-cell activation. We reported previously that the SFK LynA is uniquely susceptible to rapid ubiquitin-mediated degradation in macrophages, functioning as a rheostat regulating signaling (Freedman et al., 2015). We now report the mechanism by which LynA is preferentially targeted for degradation and how cell specificity is built into the LynA rheostat. Using genetic, biochemical, and quantitative phosphopeptide analyses, we found that the E3 ubiquitin ligase c-Cbl preferentially targets LynA via a phosphorylated tyrosine (Y32) in its unique region. This distinct mode of c-Cbl recognition depresses steady-state expression of LynA in macrophages derived from mice. Mast cells, however, express little c-Cbl and have correspondingly high LynA. Upon activation, mast-cell LynA is not rapidly degraded, and SFK-mediated signaling is amplified relative to macrophages. Cell-specific c-Cbl expression thus builds cell specificity into the LynA checkpoint., Competing Interests: BB, AJ, CG, MN, ZS, SH, PS, NH, JD, KS, TF No competing interests declared, (© 2019, Brian et al.)
- Published
- 2019
- Full Text
- View/download PDF
5. Community Engagement and Planning versus Resources for Services for Implementing Depression Quality Improvement: Exploratory Analysis for Black and Latino Adults.
- Author
-
Barceló NE, Lopez A, Tang L, Aguilera Nunez MG, Jones F, Miranda J, Chung B, Arevian A, Bonds C, Izquierdo A, Dixon E, and Wells K
- Subjects
- Adult, Black or African American statistics & numerical data, Female, Hispanic or Latino statistics & numerical data, Humans, Male, Mental Health, Middle Aged, Minority Groups psychology, Program Development, Quality Improvement, Quality of Life psychology, Black or African American psychology, Community Mental Health Services methods, Depression ethnology, Depression prevention & control, Hispanic or Latino psychology
- Abstract
Objective: Racial/ethnic minorities experience disparities in depression1 and there is a paucity of evidence-based interventions to improve depression care access and outcomes. Community Partners in Care (CPIC) is a community-partnered study of depression care quality improvement (QI) in under-resourced, urban communities: Community Engagement and Planning (CEP) for multi-sector coalitions, and Resources for Services (RS) for program technical assistance.2 CEP demonstrated benefits for the overall CPIC study population; effects for Black and Latino sub-populations are unknown., Methods: This sub-analysis examines outcomes for 409 Latino and 488 Black (non-Latino) adults recruited from 90 programs who completed baseline or 6-month follow-up. Regression analyses were used to estimate CEP vs RS intervention effects on primary (Mental Health Related Quality of Life [MHRQL], Patient Health Questionnaire-9 [PHQ-9]) and community-prioritized (mental wellness, physical activity, risk for homelessness) outcomes at 6-months., Results: Baseline characteristics did not differ significantly by intervention in either group. In the adjusted analysis for Black adults, CEP resulted in decreased odds of poor MHRQL (OR: .62, 95% CI=.41-.94, P=.028) with a trend for reducing homelessness risk (OR: .60, .35-1.05, P=.69). For Latino adults, CEP resulted in greater probability of mental wellness (OR: 1.81, 1.05-3.13, P=.034) and a trend for increased physical activity (OR: 1.52, .93-2.49, P=.091)., Conclusions: Exploratory analyses of CEP for depression quality improvement suggests significant 6-month benefits in mental health outcomes for Black and Latino participants and trends for improvement in community-prioritized outcomes for both groups. Findings may inform research in multi-sector coalitions to promote equity in depression care., Competing Interests: Competing Interests: None declared.
- Published
- 2019
- Full Text
- View/download PDF
6. Community Interventions to Promote Mental Health and Social Equity.
- Author
-
Castillo EG, Ijadi-Maghsoodi R, Shadravan S, Moore E, Mensah MO 3rd, Docherty M, Aguilera Nunez MG, Barcelo N, Goodsmith N, Halpin LE, Morton I, Mango J, Montero AE, Rahmanian Koushkaki S, Bromley E, Chung B, Jones F, Gabrielian S, Gelberg L, Greenberg JM, Kalofonos I, Kataoka SH, Miranda J, Pincus HA, Zima BT, and Wells KB
- Subjects
- Humans, Psychotic Disorders prevention & control, Psychotic Disorders therapy, Schools, Community Medicine, Health Promotion, Mental Health, Social Environment
- Abstract
Purpose of Review: We review recent community interventions to promote mental health and social equity. We define community interventions as those that involve multi-sector partnerships, emphasize community members as integral to the intervention, and/or deliver services in community settings. We examine literature in seven topic areas: collaborative care, early psychosis, school-based interventions, homelessness, criminal justice, global mental health, and mental health promotion/prevention. We adapt the social-ecological model for health promotion and provide a framework for understanding the actions of community interventions., Recent Findings: There are recent examples of effective interventions in each topic area. The majority of interventions focus on individual, family/interpersonal, and program/institutional social-ecological levels, with few intervening on whole communities or involving multiple non-healthcare sectors. Findings from many studies reinforce the interplay among mental health, interpersonal relationships, and social determinants of health. There is evidence for the effectiveness of community interventions for improving mental health and some social outcomes across social-ecological levels. Studies indicate the importance of ongoing resources and training to maintain long-term outcomes, explicit attention to ethics and processes to foster equitable partnerships, and policy reform to support sustainable healthcare-community collaborations.
- Published
- 2019
- Full Text
- View/download PDF
7. Biomarkers of inflammation in infants with cystic fibrosis.
- Author
-
Laguna TA, Williams CB, Nunez MG, Welchlin-Bradford C, Moen CE, Reilly CS, and Wendt CH
- Subjects
- Biomarkers metabolism, Bronchoalveolar Lavage Fluid, Child, Preschool, Cohort Studies, Female, Humans, Infant, Infant, Newborn, Inflammation diagnosis, Inflammation metabolism, Longitudinal Studies, Male, Neutrophils metabolism, Prospective Studies, Sputum metabolism, Cystic Fibrosis diagnosis, Cystic Fibrosis metabolism, Neonatal Screening trends
- Abstract
Background: There are urgent needs for clinically relevant biomarkers to identify children with cystic fibrosis (CF) at risk for more progressive lung disease and to serve as outcome measures for clinical trials. Our objective was to investigate three targeted biomarkers in a population of asymptomatic CF infants., Methods: Urine, blood and lung function data were collected for 2 years from clinically stable infants diagnosed with CF by newborn screening. A subset of CF infants had bronchoscopy with lavage performed at 6 months and 1 year. Urine was collected quarterly from healthy control infants. Expectorated sputum and urine were collected quarterly for 2 years from clinically stable CF adults. Desmosine, club cell secretory protein (CCSP) and cathepsin B concentrations were measured and compared. Mixed effects models were used to identify associations between biomarker concentrations and clinical characteristics. Receiver operator characteristic curves were generated to investigate the sensitivity and specificity of the biomarkers., Results: Urinary cathepsin B was significantly higher in CF infants compared to healthy infants (p = 0.005). CF infant airway and urinary cathepsin B concentrations were significantly lower compared to adult CF subjects (p = 0.002 & p = 0.022, respectively). CF infant airway CCSP was significantly higher than adult CF subjects (p < 0.001). There was a significant correlation between CF infant plasma CCSP and BALF CCSP (p = 0.046). BALF CCSP was negatively associated with IL-8 (p = 0.017). There was no correlation between biomarker concentration and FEV
0.5 ., Conclusions: Cathepsin B and CCSP show promise as biomarkers of inflammation in CF infants. Further study is needed.- Published
- 2018
- Full Text
- View/download PDF
8. Modulation of musculoskeletal hyperalgesia by brown adipose tissue activity in mice.
- Author
-
Goudie-DeAngelis EM, Abdelhamid RE, Nunez MG, Kissel CL, Kovács KJ, Portoghese PS, and Larson AA
- Subjects
- Adipose Tissue, Brown drug effects, Adrenergic beta-Agonists toxicity, Animals, Body Temperature drug effects, Body Temperature genetics, Body Weight drug effects, Body Weight genetics, Cold Temperature adverse effects, Disease Models, Animal, Ethanolamines toxicity, Female, Hyperalgesia genetics, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Muscle Strength drug effects, Musculoskeletal Pain pathology, Musculoskeletal Pain surgery, Pain Threshold drug effects, Pain Threshold physiology, Reaction Time drug effects, Reaction Time physiology, Swimming psychology, Tail innervation, Uncoupling Protein 1 deficiency, Uncoupling Protein 1 genetics, Adipose Tissue, Brown pathology, Hyperalgesia etiology, Hyperalgesia pathology, Musculoskeletal Pain complications
- Abstract
Cold exposure and a variety of types of mild stress increase pain in patients with painful disorders such as fibromyalgia syndrome. Acutely, stress induces thermogenesis by increasing sympathetic activation of beta-3 (β3) adrenergic receptors in brown adipose tissue. Chronic stress leads to the hypertrophy of brown adipose, a phenomenon termed adaptive thermogenesis. Based on the innervation of skeletal muscle by collaterals of nerves projecting to brown adipose, we theorized an association between brown adipose tissue activity and musculoskeletal hyperalgesia and tested this hypothesis in mice. Exposure to a cold swim or injection of BRL37344 (β3 adrenergic agonist) each enhanced musculoskeletal hyperalgesia, as indicated by morphine-sensitive decreases in grip force responses, whereas SR59230A (β3 adrenergic antagonist) attenuated swim-induced hyperalgesia. Chemical ablation of interscapular brown adipose, using Rose Bengal, attenuated the development of hyperalgesia in response to either swim stress or BRL37344. In addition, elimination of the gene expressing uncoupling protein-1 (UCP1), the enzyme responsible for thermogenesis, prevented musculoskeletal hyperalgesia in response to either a swim or BRL37344, as documented in UCP1-knockout (UCP1-KO) mice compared with wild-type controls. Together, these data provide a convergence of evidence suggesting that activation of brown adipose contributes to stress-induced musculoskeletal hyperalgesia., Competing Interests: The authors report no conflict of interest with any known agency.
- Published
- 2016
- Full Text
- View/download PDF
9. Intrathecal urocortin I in the spinal cord as a murine model of stress hormone-induced musculoskeletal and tactile hyperalgesia.
- Author
-
Larson AA, Nunez MG, Kissel CL, and Kovács KJ
- Subjects
- Acenaphthenes administration & dosage, Animals, Disease Models, Animal, Dose-Response Relationship, Drug, Female, Injections, Spinal, Mice, Nociception drug effects, Nociception physiology, Pain Measurement, Peptide Fragments administration & dosage, Peptides, Cyclic administration & dosage, Receptors, Corticotropin-Releasing Hormone antagonists & inhibitors, Receptors, Corticotropin-Releasing Hormone physiology, Stress, Psychological complications, Corticotropin-Releasing Hormone administration & dosage, Hand Strength, Hyperalgesia chemically induced, Hyperalgesia physiopathology, Spinal Cord drug effects, Stress, Psychological chemically induced, Urocortins administration & dosage
- Abstract
Stress is antinociceptive in some models of pain, but enhances musculoskeletal nociceptive responses in mice and muscle pain in patients with fibromyalgia syndrome. To test the hypothesis that urocortins are stress hormones that are sufficient to enhance tactile and musculoskeletal hyperalgesia, von Frey fibre sensitivity and grip force after injection of corticotropin-releasing factor (CRF), urocortin I and urocortin II were measured in mice. Urocortin I (a CRF1 and CRF2 receptor ligand) produced hyperalgesia in both assays when injected intrathecally (i.t.) but not intracerebroventricularly, and only at a large dose when injected peripherally, suggesting a spinal action. Morphine inhibited urocortin I-induced changes in nociceptive responses in a dose-related fashion, confirming that changes in behaviour reflect hyperalgesia rather than weakness. No tolerance developed to the effect of urocortin I (i.t.) when injected repeatedly, consistent with a potential to enhance pain chronically. Tactile hyperalgesia was inhibited by NBI-35965, a CRF1 receptor antagonist, but not astressin 2B, a CRF2 receptor antagonist. However, while urocortin I-induced decreases in grip force were not observed when co-administered i.t. with either NBI-35965 or astressin 2B, they were even more sensitive to inhibition by astressin, a non-selective CRF receptor antagonist. Together these data indicate that urocortin I acts at CRF receptors in the mouse spinal cord to elicit a reproducible and persistent tactile (von Frey) and musculoskeletal (grip force) hyperalgesia. Urocortin I-induced hyperalgesia may serve as a screen for drugs that alleviate painful conditions that are exacerbated by stress., (© 2015 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.)
- Published
- 2015
- Full Text
- View/download PDF
10. After a cold conditioning swim, UCP2-deficient mice are more able to defend against the cold than wild type mice.
- Author
-
Abdelhamid RE, Kovács KJ, Nunez MG, and Larson AA
- Subjects
- Animals, Body Temperature physiology, Ion Channels genetics, Mice, Mice, Knockout, Mitochondrial Proteins genetics, Swimming, Uncoupling Protein 2, Body Temperature Regulation physiology, Cold Temperature, Ion Channels metabolism, Mitochondrial Proteins metabolism, Muscle, Skeletal metabolism, Thermogenesis physiology
- Abstract
Uncoupling protein 2 (UCP2) is widely distributed throughout the body including the brain, adipose tissue and skeletal muscles. In contrast to UCP1, UCP2 does not influence resting body temperature and UCP2-deficient (-/-) mice have normal thermoregulatory responses to a single exposure to cold ambient temperatures. Instead, UCP2-deficient mice are more anxious, exhibit anhedonia and have higher circulating corticosterone than wild type mice. To test the possible role of UCP2 in depressive behavior we exposed UCP2-deficient and wild type mice to a cold (26°C) forced swim and simultaneously measured rectal temperatures during and after the swim. The time that UCP2-deficient mice spent immobile did not differ from wild type mice and all mice floated more on day 2. However, UCP2-deficient mice were more able to defend against the decrease in body temperature during a second daily swim at 26°C than wild type mice (area under the curve for wild type mice: 247.0±6.4; for UCP2-deficient mice: 284.4±3.8, P<0.0001, Student's t test). The improved thermoregulation of wild type mice during a second swim at 26°C correlated with their greater immobility whereas defense against the warmth during a swim at 41°C correlated better with greater immobility of UCP2-deficient mice. Together these data indicate that while the lack of UCP2 has no acute effect on body temperature, UCP2 may inhibit rapid improvements in defense against cold, in contrast to UCP1, whose main function is to promote thermogenesis., (Copyright © 2014. Published by Elsevier Inc.)
- Published
- 2014
- Full Text
- View/download PDF
11. Depressive behavior in the forced swim test can be induced by TRPV1 receptor activity and is dependent on NMDA receptors.
- Author
-
Abdelhamid RE, Kovács KJ, Nunez MG, and Larson AA
- Subjects
- Amitriptyline pharmacology, Animals, Antidepressive Agents pharmacology, Body Temperature, Diterpenes pharmacology, Hot Temperature, Male, Mice, Stress, Physiological, Stress, Psychological, Swimming, TRPV Cation Channels agonists, Behavior, Animal physiology, Depression physiopathology, Depression psychology, Receptors, N-Methyl-D-Aspartate physiology, TRPV Cation Channels physiology
- Abstract
Blocking, desensitizing, or knocking out transient receptor potential vanilloid type 1 (TRPV1) receptors decreases immobility in the forced swim test, a measure of depressive behavior. We questioned whether enhancing TRPV1 activity promotes immobility in a fashion that is prevented by antidepressants. To test this we activated heat-sensitive TRPV1 receptors in mice by water that is warmer than body temperature (41 °C) or a low dose of resiniferatoxin (RTX). Water at 41 °C elicited less immobility than cooler water (26 °C), indicating that thermoregulatory sites do not contribute to immobility. Although a desensitizing regimen of RTX (3-5 injections of 0.1 mg/kg s.c.) decreased immobility during swims at 26 °C, it did not during swims at 41 °C. In contrast, low dose of RTX (0.02 mg/kg s.c.) enhanced immobility, but only during swims at 41 °C. Thus, activation of TRPV1 receptors, endogenously or exogenously, enhances immobility and these sites are activated by cold rather than warmth. Two distinct types of antidepressants, amitriptyline (10mg/kg i.p.) and ketamine (50 mg/kg i.p.), each inhibited the increase in immobility induced by the low dose of RTX, verifying its mediation by TRPV1 sites. When desensitization was limited to central populations using intrathecal injections of RTX (0.25 μg/kg i.t.), immobility was attenuated at both temperatures and the increase in immobility produced by the low dose of RTX was inhibited. This demonstrates a role for central TRPV1 receptors in depressive behavior, activated by conditions (cold stress) distinct from those that activate TRPV1 receptors along thermosensory afferents (heat)., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
12. Resiniferatoxin (RTX) causes a uniquely protracted musculoskeletal hyperalgesia in mice by activation of TRPV1 receptors.
- Author
-
Abdelhamid RE, Kovács KJ, Honda CN, Nunez MG, and Larson AA
- Subjects
- Animals, Female, Hyperalgesia chemically induced, Mice, Musculoskeletal Pain chemically induced, Pain Measurement drug effects, Diterpenes toxicity, Hyperalgesia metabolism, Musculoskeletal Pain metabolism, Neurotoxins toxicity, Pain Measurement methods, TRPV Cation Channels metabolism
- Abstract
Unlabelled: Inactivation of transient receptor potential vanilloid-1 (TRPV1) receptors is one approach to analgesic drug development. However, TRPV1 receptors exert different effects on each modality of pain. Because muscle pain is clinically important, we compared the effect of TRPV1 ligands on musculoskeletal nociception to that on thermal and tactile nociception. Injected parenterally, capsaicin had no effect on von Frey fiber responses (tactile) but induced a transient hypothermia and hyperalgesia in both the tail flick (thermal) and grip force (musculoskeletal) assays, presumably by its agonistic action at TRPV1 sites. In contrast, resiniferatoxin (RTX) produced a chronic (>58 days) thermal antinociception, consistent with its reported ability to desensitize TRPV1 sites. In the same mice, RTX produced a transient hypothermia (7 hours) and a protracted (28-day) musculoskeletal hyperalgesia in spite of a 35.5% reduction in TRPV1 receptor immunoreactivity in muscle afferents. Once musculoskeletal hyperalgesia subsided, mice were tolerant to the hyperalgesic effects of either capsaicin or RTX whereas tolerance to hypothermia did not develop until after 3 injections. Musculoskeletal hyperalgesia was prevented but not reversed by SB-366791, a TRPV1 antagonist, indicating that TRPV1 receptors initiate but do not maintain hyperalgesia. Injected intrathecally, RTX produced only a brief musculoskeletal hyperalgesia (2 days), after which mice were tolerant to this effect., Perspective: The effect of TRPV1 receptors varies depending on modality and tissue type, such that RTX causes thermal antinociception, musculoskeletal hyperalgesia, and no effect on tactile nociception in healthy mice. Spinal TRPV1 receptors are a potential target for pain relief as they induce only a short musculoskeletal hyperalgesia followed by desensitization., (Copyright © 2013 American Pain Society. Published by Elsevier Inc. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
13. Forced swim-induced musculoskeletal hyperalgesia is mediated by CRF2 receptors but not by TRPV1 receptors.
- Author
-
Abdelhamid RE, Kovacs KJ, Pasley JD, Nunez MG, and Larson AA
- Subjects
- Acenaphthenes therapeutic use, Analgesics therapeutic use, Analysis of Variance, Animals, Body Weight drug effects, Cold Temperature adverse effects, Disease Models, Animal, Diterpenes therapeutic use, Female, Hyperalgesia drug therapy, Mice, Morphine therapeutic use, Muscle Strength drug effects, Pain Measurement, Peptide Fragments therapeutic use, Peptides, Cyclic therapeutic use, Reaction Time drug effects, Receptors, Corticotropin-Releasing Hormone antagonists & inhibitors, Swimming psychology, TRPV Cation Channels antagonists & inhibitors, Hyperalgesia etiology, Hyperalgesia metabolism, Musculoskeletal Pain etiology, Musculoskeletal Pain metabolism, Receptors, Corticotropin-Releasing Hormone metabolism, TRPV Cation Channels metabolism
- Abstract
The exacerbation of musculoskeletal pain by stress in humans is modeled by the musculoskeletal hyperalgesia in rodents following a forced swim. We hypothesized that stress-sensitive corticotropin releasing factor (CRF) receptors and transient receptor vanilloid 1 (TRPV1) receptors are responsible for the swim stress-induced musculoskeletal hyperalgesia. We confirmed that a cold swim (26 °C) caused a transient, morphine-sensitive decrease in grip force responses reflecting musculoskeletal hyperalgesia in mice. Pretreatment with the CRF2 receptor antagonist astressin 2B, but not the CRF1 receptor antagonist NBI-35965, attenuated this hyperalgesia. Desensitizing the TRPV1 receptor centrally or peripherally using desensitizing doses of resiniferatoxin (RTX) failed to prevent the musculoskeletal hyperalgesia produced by cold swim. SB-366791, a TRPV1 antagonist, also failed to influence swim-induced hyperalgesia. Together these data indicate that swim stress-induced musculoskeletal hyperalgesia is mediated, in part, by CRF2 receptors but is independent of the TRPV1 receptor., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.