Search

Your search keyword '"Nunes, Rodrigo D."' showing total 15 results
Start Over Author "Nunes, Rodrigo D."
15 results on '"Nunes, Rodrigo D."'

1. Beyond the eye: Kynurenine pathway impairment causes midgut homeostasis dysfunction and survival and reproductive costs in blood-feeding mosquitoes

Author

Bottino-Rojas, Vanessa, Ferreira-Almeida, Igor, Nunes, Rodrigo D, Feng, Xuechun, Pham, Thai Binh, Kelsey, Adam, Carballar-Lejarazú, Rebeca, Gantz, Valentino, Oliveira, Pedro L, and James, Anthony A

Subjects
Biological Sciences, Genetics, Rare Diseases, Infectious Diseases, Emerging Infectious Diseases, Vector-Borne Diseases, Good Health and Well Being, Aedes, Animals, Culex, Female, Homeostasis, Kynurenine, Mosquito Vectors, Tryptophan, Tryptophan metabolism, Transgenesis, Kynurenine hydroxylase, monooxygenase, Kynurenine hydroxylase/monooxygenase, Medicinal and Biomolecular Chemistry, Biochemistry and Cell Biology, Zoology, Entomology, Biochemistry and cell biology
Abstract

Insect ommochrome biosynthesis pathways metabolize tryptophan to generate eye-color pigments and wild-type alleles of pathway genes are useful phenotypic markers in transgenesis studies. Pleiotropic effects of mutations in some genes exert a load on both survival and reproductive success in blood-feeding species. Here, we investigated the challenges imposed on mosquitoes by the increase of tryptophan metabolites resulting from blood meal digestion and the impact of disruptions of the ommochrome biosynthesis pathway. Female mosquitoes with spontaneous and induced mutations in the orthologs of the genes encoding kynurenine hydroxylase in Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus exhibited impaired survival and reproductive phenotypes that varied in type and severity among the species. A compromised midgut permeability barrier function was also observed in An. stephensi. Surprisingly, mutant mosquitoes displayed an increase in microbiota compared to controls that was not accompanied by a general induction of immune genes. Antibiotic treatment rescued some deleterious traits implicating a role for the kynurenine pathway (KP) in midgut homeostasis. Supplemental xanthurenic acid, a KP end-product, rescued lethality and limited microbiota proliferation in Ae. aegypti. These data implicate the KP in the regulation of the host/microbiota interface. These pleiotropic effects on mosquito physiology are important in the development of genetic strategies targeting vector mosquitoes.

Published
2022

2. Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection

Author

Mesquita, Rafael D., Vionette-Amaral, Raquel J., Lowenberger, Carl, Rivera-Pomar, Rolando, Monteiro, Fernando A., Minx, Patrick, Spieth, John, Carvalho, A. Bernardo, Panzera, Francisco, Lawson, Daniel, Torres, André Q., Ribeiro, Jose M. C., Sorgine, Marcos H. F., Waterhouse, Robert M., Montague, Michael J., Abad-Franch, Fernando, Alves-Bezerra, Michele, Amaral, Laurence R., Araujo, Helena M., Araujo, Ricardo N., Aravind, L., Atella, Georgia C., Azambuja, Patricia, Berni, Mateus, Bittencourt-Cunha, Paula R., Braz, Gloria R. C., Calderón-Fernández, Gustavo, Carareto, Claudia M. A., Christensen, Mikkel B., Costa, Igor R., Costa, Samara G., Dansa, Marilvia, Daumas-Filho, Carlos R. O., De-Paula, Iron F., Dias, Felipe A., Dimopoulos, George, Emrich, Scott J., Esponda-Behrens, Natalia, Fampa, Patricia, Fernandez-Medina, Rita D., da Fonseca, Rodrigo N., Fontenele, Marcio, Fronick, Catrina, Fulton, Lucinda A., Gandara, Ana Caroline, Garcia, Eloi S., Genta, Fernando A., Giraldo-Calderón, Gloria I., Gomes, Bruno, Gondim, Katia C., Granzotto, Adriana, Guarneri, Alessandra A., Guigó, Roderic, Harry, Myriam, Hughes, Daniel S. T., Jablonka, Willy, Jacquin-Joly, Emmanuelle, Juárez, M. Patricia, Koerich, Leonardo B., Lange, Angela B., Latorre-Estivalis, José Manuel, Lavore, Andrés, Lawrence, Gena G., Lazoski, Cristiano, Lazzari, Claudio R., Lopes, Raphael R., Lorenzo, Marcelo G., Lugon, Magda D., Majerowicz, David, Marcet, Paula L., Mariotti, Marco, Masuda, Hatisaburo, Megy, Karine, Melo, Ana C. A., Missirlis, Fanis, Mota, Theo, Noriega, Fernando G., Nouzova, Marcela, Nunes, Rodrigo D., Oliveira, Raquel L. L., Oliveira-Silveira, Gilbert, Ons, Sheila, Orchard, Ian, Pagola, Lucia, Paiva-Silva, Gabriela O., Pascual, Agustina, Pavan, Marcio G., Pedrini, Nicolás, Peixoto, Alexandre A., Pereira, Marcos H., Pike, Andrew, Polycarpo, Carla, Prosdocimi, Francisco, Ribeiro-Rodrigues, Rodrigo, Robertson, Hugh M., Salerno, Ana Paula, Salmon, Didier, Santesmasses, Didac, Schama, Renata, Seabra-Junior, Eloy S., Silva-Cardoso, Livia, Silva-Neto, Mario A. C., Souza-Gomes, Matheus, Sterkel, Marcos, Taracena, Mabel L., Tojo, Marta, Tu, Zhijian Jake, Tubio, Jose M. C., Ursic-Bedoya, Raul, Venancio, Thiago M., Walter-Nuno, Ana Beatriz, Wilson, Derek, Warren, Wesley C., Wilson, Richard K., Huebner, Erwin, Dotson, Ellen M., and Oliveira, Pedro L.

Published
2015

3. Beyond the eye: Kynurenine pathway impairment causes midgut homeostasis dysfunction and survival and reproductive costs in blood-feeding mosquitoes

Author

Bottino-Rojas, Vanessa, primary, Ferreira-Almeida, Igor, additional, Nunes, Rodrigo D., additional, Feng, Xuechun, additional, Pham, Thai Binh, additional, Kelsey, Adam, additional, Carballar-Lejarazú, Rebeca, additional, Gantz, Valentino, additional, Oliveira, Pedro L., additional, and James, Anthony A., additional

Published
2022
Full Text
View/download PDF

5. Aedes aegypti post-emergence transcriptome: Unveiling the molecular basis for the hematophagic and gonotrophic capacitation

Author

de Carvalho, Stephanie S., primary, Rodovalho, Cynara M., additional, Gaviraghi, Alessandro, additional, Mota, Maria Beatriz S., additional, Jablonka, Willy, additional, Rocha-Santos, Carlúcio, additional, Nunes, Rodrigo D., additional, Sá-Guimarães, Thayane da Encarnação, additional, Oliveira, Daniele S., additional, Melo, Ana C. A., additional, Moreira, Monica F., additional, Fampa, Patrícia, additional, Oliveira, Marcus F., additional, da Silva-Neto, Mario Alberto C., additional, Mesquita, Rafael D., additional, and Atella, Georgia C., additional

Published
2021
Full Text
View/download PDF

9. Erratum to :'Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection' (vol 112, pg 14936, 2015)

Author

Lowenberger, Carl, Rivera Pomar, Rolando, Monteiro, Fernando A., Minx, Patrick, Spieth, John, Bernardo Carvalho, A., Panzera, Francisco, Lawson, Daniel, Torres, Andre Q., Ribeiro, Jose M. C., Sorgine, Marcos H. F., Waterhouse, Robert M., Montague, Michael J., Abad Franch, Fernando, Alves Bezerra, Michele, Amaral, Laurence R., Araujo, Helena M., Araujo, Ricardo N., Aravind, L., Atella, Georgia C., Azambuja, Patricia, Berni, Mateus, Bittencourt Cunha, Paula R., Braz, Gloria R. C., Calderon Fernandez, Gustavo, Carareto, Claudia M. A., Christensen, Mikkel B., Costa, Igor R., Costa, Samara G., Dansa, Marilvia, Daumas Filho, Carlos R. O., De Paula, Iron F., Dias, Felipe A., Dimopoulos, George, Emrich, Scott J., Esponda Behrens, Natalia, Fampa, Patricia, Fernandez Medina, Rita D., da Fonseca, Rodrigo N., Fontenele, Marcio, Fronick, Catrina, Fulton, Lucinda A., Gandara, Ana Caroline, Garcia, Eloi S., Genta, Fernando A., Giraldo Calderón, Gloria I., Gomes, Bruno, Gondim, Katia C., Granzotto, Adriana, Guarneri, Alessandra A., Guigó, Roderic, Harry, Myriam, Hughes, Daniel S. T., Jablonka, Willy, Jacquin Joly, Emmanuelle, Patricia Juarez, M., Koerich, Leonardo B., Latorre Estivalis, Jose Manuel, Lavore, Andrés, Lawrence, Gena G., Lazoski, Cristiano, Lazzari, Claudio R., Lopes, Raphael R., Lorenzo, Marcelo G., Lugon, Magda D., Majerowicz, David, Marcet, Paula L., Mariotti, Marco, Masuda, Hatisaburo, Megy, Karine, Melo, Ana C.A., Missirlis, Fanis, Mota, Theo, Noriega, Fernando G., Nouzova, Marcela, Nunes, Rodrigo D., Oliveira, Raquel L.L., Oliveira Silveira, Gilbert, Ons, Sheila, Pagola, Lucia, Paiva Silva, Gabriela O., Pascual, Agustina, Pavan, Marcio G., Pedrini, Nicolas, Peixoto, Alexandre A., Pereira, Marcos H., Pike, Andrew, Polycarpo, Carla, Prosdocimi, Francisco, Ribeiro Rodrigues, Rodrigo, Robertson, Hugh M., Salerno, Ana Paula, Salmon, Didier, Santesmasses, Didac, Schama, Renata, Seabra Junior, Eloy S., Silva Cardoso, Livia, Mesquita, Rafael D., and Vionette Amaral, Raquel J.

Subjects
rhodnius prolixus, Rhodnius prolixus, genome, hematophagy, immunity, Chagas disease, maladie de chagas
Abstract

Rhodnius prolixus not only has served as a model organism for the study of insect physiology, but also is a major vector of Chagas disease, an illness that affects approximately seven million people worldwide. We sequenced the genome of R. prolixus, generated assembled sequences covering 95% of the genome (similar to 702 Mb), including 15,456 putative protein-coding genes, and completed comprehensive genomic analyses of this obligate blood-feeding insect. Although immune-deficiency (IMD)-mediated immune responses were observed, R. prolixus putatively lacks key components of the IMD pathway, suggesting a reorganization of the canonical immune signaling network. Although both Toll and IMD effectors controlled intestinal microbiota, neither affected Trypanosoma cruzi, the causal agent of Chagas disease, implying the existence of evasion or tolerance mechanisms. R. prolixus has experienced an extensive loss of selenoprotein genes, with its repertoire reduced to only two proteins, one of which is a selenocysteine-based glutathione peroxidase, the first found in insects. The genome contained actively transcribed, horizontally transferred genes from Wolbachia sp., which showed evidence of codon use evolution toward the insect use pattern. Comparative protein analyses revealed many lineage-specific expansions and putative gene absences in R. prolixus, including tandem expansions of genes related to chemoreception, feeding, and digestion that possibly contributed to the evolution of a blood-feeding lifestyle. The genome assembly and these associated analyses provide critical information on the physiology and evolution of this important vector species and should be instrumental for the development of innovative disease control methods.

Published
2016

12. Amino acids trigger down-regulation of superoxide via TORC pathway in the midgut of Rhodnius prolixus

Author

Gandara, Ana Caroline P., primary, Oliveira, José Henrique M., additional, Nunes, Rodrigo D., additional, Goncalves, Renata L.S., additional, Dias, Felipe A., additional, Hecht, Fabio, additional, Fernandes, Denise C., additional, Genta, Fernando A., additional, Laurindo, Francisco R.M., additional, Oliveira, Marcus F., additional, and Oliveira, Pedro L., additional

Published
2016
Full Text
View/download PDF

13. The Antioxidant Role of Xanthurenic Acid in the Aedes aegypti Midgut during Digestion of a Blood Meal

Author

Lima, Vitor L. A., primary, Dias, Felipe, additional, Nunes, Rodrigo D., additional, Pereira, Luiza O., additional, Santos, Tiago S. R., additional, Chiarini, Luciana B., additional, Ramos, Tadeu D., additional, Silva-Mendes, Bernardo J., additional, Perales, Jonas, additional, Valente, Richard H., additional, and Oliveira, Pedro L., additional

Published
2012
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results