1. FOXM1 Aptamer-Polyethylenimine Nanoplatform Coated With Hyaluronic Acid And AS1411 Aptamer For Dual-Targeted Delivery of Doxorubicin And Synergistic Treatment of Tumor Cells.
- Author
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Khademi, Zahra, Yazdi, Katayoon Sarafraz, Ramezani, Mohammad, Alibolandi, Mona, Rezvani, Seyede Aysa, Abnous, Khalil, and Taghdisi, Seyed Mohammad
- Subjects
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HYALURONIC acid , *CANCER cells , *NUCLEOLIN , *FLUORIMETRY , *CELL imaging , *DOXORUBICIN - Abstract
The objective of this investigation was to develop a self-assembled, dual-functionalized delivery system that could effectively transport doxorubicin (DOX) to cancer cells through the use of AS1411 aptamer and hyaluronic acid polymer (HA). The ultimate goal is an improved targeting approach for more efficient treatment. The core of this system comprised polyethylenimine (PEI) and FOXM1 aptamer, which was coated by HA. Next, nucleolin targeting aptamers (AS1411) were loaded onto the nanocomplex. Afterward, DOX was added to Aptamers (Apts)-HA-PEI-FOXM1 NPs to create the DOX-AS1411-HA-PEI-FOXM1 NPs for better treatment of cancer cells. The cytotoxic effect of the nanocomplex on L929, 4T1, and A549 cells showed that cell mortality in target cancer cells (4T1 and A549) was considerably enhanced compared to nontarget cells (L929, normal cells). The findings from the flow cytometry analysis and fluorescence imaging demonstrated the cellular absorption of DOX-Apts-HA-PEI-FOXM1 NPs in target cells was significantly enhanced when compared to L929 cells. Furthermore, in vivo antitumor study exhibited that DOX-Apts-HA-PEI-FOXM1 NPs rendered specific tumor accumulation and increasing of the anti-tumor effects. [Display omitted] • Dual-functionalized delivery system was developed for delivery of DOX and FOXM1 apt. • The core of this system comprised PEI and FOXM1 apt. • The use of AS1411 apt and HA guaranteed the targeted delivery of NPs to cancer cells. • Cellular internalization of DOX-Apts-HA-PEI-FOXM1 NPs into cancer cells was high. • DOX-Apts-HA-PEI-FOXM1 NPs rendered specific tumor accumulation in vivo. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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