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4 results on '"Nucci, C. G."'

1. Three-Dimensional High-Definition Ventriculoscope: Single-Center Case Series

Author

Doglietto, Francesco, Belotti, F., Nucci, C. G., Roca, E., Mattogno, Pier Paolo, Zappa, F., Migliorati, K., Panciani, P. P., Spena, G., Cereda, C., Cornali, C., Fontanella, Marco Maria, Doglietto F. (ORCID:0000-0002-7438-0734), Mattogno P. P., Fontanella M. M., Doglietto, Francesco, Belotti, F., Nucci, C. G., Roca, E., Mattogno, Pier Paolo, Zappa, F., Migliorati, K., Panciani, P. P., Spena, G., Cereda, C., Cornali, C., Fontanella, Marco Maria, Doglietto F. (ORCID:0000-0002-7438-0734), Mattogno P. P., and Fontanella M. M.

Abstract

Objective: Three-dimensional (3D), high-definition (HD) endoscopy has been recently introduced in neurosurgery, and its value has been discussed extensively in endonasal skull base surgery. Because there has been no reported clinical series on the use of a recent 3D-HD ventriculoscope, the aim of this study was to describe our initial experience with this novel device. Methods: Patients consecutively operated on from June 2016 to June 2018 with a 3D-HD ventriculoscope were prospectively collected. The system is a 6-mm, 0-degree optic with a 105-degree field of view, with a central working channel of 2.2-mm diameter and 2 side channels of 1.3-mm diameter. Patients' demographic data, preoperative symptoms, and neurologic status; neuroradiologic data; type of surgery; operative time; intraoperative and postoperative complications, and follow-up data were prospectively recorded and retrospectively reviewed. Results: Twenty-four patients (age range: 3−84 years) underwent 25 procedures including endoscopic third ventriculocisternostomy, biopsy, and cyst fenestration. The technical goal of surgery was obtained in all patients. There were no intraoperative complications, expect for 1 intraoperative epileptic seizure. Postoperative complications included asymptomatic subdural collections in 2 patients, infection, and delayed endoscopic third ventriculocisternostomy closure in 1 patient each. Relative limits of the system are its size and the availability of only a 0-degree optic. Image quality appeared satisfactory in all procedures. The lack of a dedicated introducer was resolved, exploiting a vascular “peel-away” system. Conclusions: 3D-HD technology seems to provide potential advantages in ventricular surgery. This initial experience is promising but must be confirmed by larger series.

Published
2019

4. Comparative evaluation of genome-wide gene expression profiles in ruptured and unruptured human intracranial aneurysms.

Author

Marchese E, Vignati A, Albanese A, Nucci CG, Sabatino G, Tirpakova B, Lofrese G, Zelano G, and Maira G

Subjects
Adult, Aged, Aneurysm, Ruptured metabolism, Aneurysm, Ruptured surgery, Apoptosis genetics, Cerebral Arteries pathology, Cerebral Arteries surgery, Chromosome Mapping, Female, Humans, Intracranial Aneurysm metabolism, Intracranial Aneurysm surgery, Male, Matrix Metalloproteinases genetics, Middle Aged, Oligonucleotide Array Sequence Analysis, Peptide Hydrolases genetics, Polymerase Chain Reaction, Protease Inhibitors, RNA genetics, RNA isolation & purification, RNA, Ribosomal genetics, Aneurysm, Ruptured genetics, Gene Expression Profiling, Genome-Wide Association Study, Intracranial Aneurysm genetics
Abstract

Few studies have evaluated the over or the underexpression of genes directly in samples of aneurysmal wall and extracranial pericranial vascular tissue to investigate the genetic influence in formation and rupture of intracranial aneurysms. We present the results obtained using the DNA microarray technique analysis on sample tissues collected during surgery. We collected and analyzed 12 aneurismal and 9 peripheral arteries (superficial temporal (STA) and middle meningeal artery (MMA) specimens from ruptured aneurysm group patients (13 cases), 10 aneurismal and 12 STA and MMA samples from unruptured aneurysm group patients (14 cases) and 5 STA and MMA artery specimens from control group patients (4 cases). Total RNA was isolated from samples and subjected to cDNA microarray analysis with the use of the human genome U133A GeneChip oligonucleotide microarray (Affymetrix, Santa Clara, CA), which allows to analyze a total number of 14,500 genes in the same time. For genes of interest, real-time RT-PCR was performed to confirm their expression level. Total RNA was isolated from samples and subjected to DNA microarray analysis with the use of the human genome U133A GeneChip oligonucleotide microarray, which allows to analyze a total number of 14,500 genes at the same time. For genes of interest, real-time RT-PCR was performed to confirm their expression level. Regarding ruptured aneurysms, genes were identified showing differential expressions (overexpressed or downregulated) pertaining to specific pathways, particularly those for the structural proteins of the extracellular matrix, members of matrix metalloproteinase (MMP) family (which resulted as being overexpressed) and genes involved in apoptotic phenomena. Particularly, real-time RT-PCR analysis confirmed the upregulation of MMP-2, MMP-9 and pro-apoptotic genes, such as Fas, Bax and Bid, and the downregulation of anti-apoptotic genes, such as Bcl-X(L) and Bcl-2. In a compared analyses of ruptured vs unruptured aneurysms, a different expression was also detected regarding gene coding the tissue inhibitor of matrix metalloproteinases 3 (TIMP-3), which appeared markedly downregulated in unruptured aneurysms, where its expression in unruptured aneurysms was similar to that observed in controls. Another gene differently expressed is nitric oxide synthase (iNOS), which appeared overexpressed in ruptured aneurysms when compared to unruptured aneurysms. Our study is the first, to our knowledge, that compares gene expression profiles (genoma-wide) in intracranial aneurysms. The results of our study suggest that the inhibitor of the metalloproteinase, the pathway of nitric oxide and the apoptotic process play a key-role in reducing the resistance of the arterial wall, that can result in formation and rupture of the intracranial aneurysms.

Published
2010

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