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In the main, convergent mixed-methods study on which this article reports, we explored Grade 12 accounting teachers' experiences of teaching in large classrooms in the Manzini region of Eswatini. As part of teachers' experiences, the methods that the teachers used to teach were also explored. Purposive sampling was used to select 25 accounting teachers from 10 schools (5 schools from rural areas and 5 schools from urban areas) who taught accounting classes with more than 35 Grade 12 learners. The selected teachers completed questionnaires for the quantitative component of the study. Purposive sampling was used to select 10 participants who were interviewed and observed on the basis of meeting the criterion set out above for the qualitative component of the study. The Statistical Package for Social Sciences (SPSS) for quantitative data analysis, and thematic analysis for qualitative data analysis were used. We found that the most dominant methods used by teachers to teach accounting in large Grade 12 classes in Eswatini were group discussions, question and answer, lecturing and demonstration methods. With the study we extended awareness of methods that teachers in emerging economies can use to cope with teaching accounting in large Grade 12 classes, and we propose further approaches to be considered to make teaching this subject in large classes sustainable.

Climate change has brought about credible changes in arid landscapes which have resulted in further challenges for pastoralists who require good quality rangeland resources to sustain their livestock. This study assessed local level coping and adaptive management strategies by pastoralists in Namibia and South Africa using a capital assets approach, and also explored non-climate related barriers that increase their vulnerability to climate change. A case study approach was used, which included focus group discussions and semi-structured interviews. The main findings of the research indicate that pastoralists have been exposed to increased temperature extremes and droughts of varying duration over time which reduced the quality and quantity of forage and resulted in losses of income and animal health. The 46 different coping and adaptation strategies used by pastoralists included financial (n = 15), natural (n = 15), human (n = 12), physical (n = 2) and social (n = 2) capital. Of these, 61% of the strategies were common between the two countries. Strategies included income diversification, supplementary feeding and livestock mobility. Pastoralists had to overcome a hierarchy of barriers to implement their coping and adaptation strategies, which were largely related to human capital in Namibia and to natural capital in South Africa. These barriers included a lack of access to grazing lands, land degradation and the loss of traditional knowledge to manage livestock and rangeland resources. Policy development should take these barriers into account while building on the foundation of existing adaptation strategies to reduce the vulnerability to climate change of pastoralists in dryland regions.

Abstract Background Hydatidosis is a parasitic infection caused by Echinococcus granulosus and humans are usually an accidental intermediate host. Involvement of the musculoskeletal system is reported to occur in 0.5% to 4% of the cases. Case presentation We present our experience with two cases of musculoskeletal hydatidosis in black African patients that required orthopaedic surgical intervention. A 51-year-old black African female presented with right hip hydatid disease and a 37-year-old black African female presented with the disease affecting the left shoulder. Both patients presented with joint pain and reduced range of motion. The patient with involvement of the shoulder had a background history of human immunodeficiency virus, this was not present with the other patient. Diagnostic work-up confirmed peri-articular hydatid disease and both patients were surgically managed with arthroplasty. Post-operative complications encountered include hardware loosening from bone lysis and hardware failure. Discussion The medical literature describes a limited number of cases of peri-articular musculoskeletal hydatid disease. Patients are often subjected to many investigations, prolonged treatment periods and multiple surgeries. Concurrent use of medical and surgical treatment is advocated however, the choice of surgery is individualised. Conclusion Hydatid disease must always feature in the differential diagnosis of multiple lytic bone lesions and radical surgical intervention may be required from the outset.

Copper nanoparticles (CuNPs) are tiny materials with special features such as high electric conductivity, catalytic activity, antimicrobial activity, and optical activity. Published reports demonstrate their utilization in various fields, including biomedical, agricultural, environmental, wastewater treatment, and sensor fields. CuNPs can be produced utilizing traditional procedures; nevertheless, such procedures have restrictions like excessive consumption of energy, low production yields, and the utilization of detrimental substances. Thus, the adoption of environmentally approachable “green” approaches for copper nanoparticle synthesis is gaining popularity. These approaches involve employing plants, bacteria, and fungi. Nonetheless, there is a scarcity of data regarding the application of microbial bioflocculants in the synthesis of copper NPs. Therefore, this review emphasizes copper NP production using microbial flocculants, which offer economic benefits and are sustainable and harmless. The review also provides a characterization of the synthesized copper nanoparticles, employing numerous analytical tools to determine their compositional, morphological, and topographical features. It focuses on scientific advances from January 2015 to December 2023 and emphasizes the use of synthesized copper NPs in wastewater treatment.

The transformation of higher education, in line with the South African Constitution, has led to increased enrolments of students with disabilities. However, limited research has been conducted on teaching and learning support these students receive. Access of students with disabilities in higher education is a human rights issue yet research shows that it has not been linked to increase in instructional support. As such, their participation has been limited. Some of the barriers limiting their participation are lecturers' negative attitudes due to lack of disability awareness and the unavailability or inadequacy of support in teaching and learning. As a result, higher education institutions have not succeeded in creating enabling teaching and learning environments for these students. This paper seeks to initiate discussion about the teaching and learning support needs of students with disabilities, particularly in an Open Distance e-Learning context and to propose strategies that can be employed to increase the quality of their participation and improve their academic outcomes.

Background: The teaching of writing in English First Additional Language (EFAL) classrooms remains less explored in the Further Education and Training Phase (FET) in South Africa. This is so despite research showing a decline in the writing skills of second language learners, especially at the FET phase, calling attention to how writing is taught. Objectives: The purpose of this study was to investigate writing approaches and teaching strategies used by teachers in teaching writing in selected EFAL FET classrooms in the Pinetown district. Method: A qualitative approach was adopted to observe five writing lessons across five schools using a video camera. Discourse analysis was used to analyse data. Results: The findings indicate that teachers mostly used a process approach to writing, which is in line with their curriculum. The study also found that teachers generally used a question and answer method to teach writing, which entails teachers controlling the interactions in the classrooms through a nomination-response cycle. Analyses of lessons also suggest that teachers creatively employed code-switching to explain writing concepts better. Conclusion: The study concludes that the effectiveness of any pedagogy depends on the teachers' knowledge and understanding of writing and approaches to writing. For effective development of learners' writing, the study recommends instruction methods that embrace collaborative writing activities in the learners' Zone of Proximal Development (ZPD) and the recognition of learners' home languages.

Television Sign Language (SL) Interpreters play a vital role in providing the Deaf community with access to information and knowledge in their primary language, Sign Language. This helps the Deaf community stay informed regarding events in their local and global environment, contributing to their development. However, a lack of research exists on SL interpreters on television. With on-screen placement of SL interpreters during news broadcasts being the primary focus in previous studies, this paper attempts to offer a unique contribution from an audience perception examination of South African Sign Language (SASL) interpreter portrayal in newscasts and its impact. The research, conducted on the SABC 1 news bulletin, employed a qualitative method and purposive sampling. Twelve participants, divided into four categories – Deaf, Hard of hearing, hearing, and SASL interpreters participated in the study through virtual interviews as data collection method. The study findings indicate the portrayal of SASL interpreters on television reflects the discrimination, inequality, and disrespect faced by the Deaf community.

Over recent years, the ‘green’ chemistry approach to synthesizing nanoparticles has made significant developments. Because of their unique features, nanoparticles have received a lot of attention. The use of a bioflocculant to promote the environmentally friendly synthesis of copper nanoparticles is described in this paper. Copper nanoparticles were biosynthesized using bioflocculant which was produced from a yeast, Pichia kudriavzevii. The chemical reduction approach was used to synthesize copper nanoparticles (CuNPs) using a bioflocculant as a capping agent. Characterization of the as-synthesized copper nanoparticles was conducted using Fourier transform infrared (FT-IR) spectroscopy, UV-visible spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy dispersive X-ray (EDX). The FT-IR spectra revealed characteristic peaks at 3267, 2956, 1656, 1059, and 511 cm−1 for the bioflocculant, while for the bioflocculant passivated CuNPs, the characteristic peaks were at 3482 (-OH), 3261, 1640, 1059, 580, and 519 cm−1 (Cu-O). These peaks revealed that functional groups such as hydroxyls, amines, and copper oxide bonds were present. The UV-Vis analysis showed surface plasmon resonance (SPR) at an absorbance range of 500–600 nm, with peak maxima at 555 and 575 nm for the as-synthesized CuNPs. The XRD pattern revealed planes such as (200) and (220) at 2θ = 43 and 52°, and the particle size (30 nm) was determined by the Debye–Scherrer equation. The transmission electron microscopy analysis revealed a spherical-shaped particle with an average size of 20 nm. The EDX analysis of the as-synthesized CuNPs revealed the presence of the element Cu, which was not present in the EDX image of the bioflocculant used in the synthesis of the CuNPs; this indicated the success of biosynthesis.

Abstract Background Alcohol use and risky drinking are significant public health problem globally. Young people, including university students, are among the most affected populations. We conducted the study to determine the prevalence and correlates of alcohol use and risky drinking among undergraduate students in the Faculty of Health Sciences at the University of the Witwatersrand, South Africa. Methods We conducted a cross-sectional study using an anonymous, self-administered online survey in REDCap. The survey questionnaire consisted of socio demographic, and alcohol use questions using the risky drinking identification screening tool (AUDIT-C). We performed descriptive statistics, bivariate and multivariable logistic regression to determine factors associated with alcohol use and risky drinking. The p-value of

Vaccination using live attenuated vaccines (LAVs) is considered the most effective method for control of lumpy skin disease (LSD). However, this method is limited by safety concerns, with reports of adverse reactions following vaccination. This study evaluates A27L and L1R which are essential proteins for virus attachment and membrane fusion as recombinant sub-unit vaccines against LSD. These proteins were recombinantly expressed in Escherichia coli and purified using affinity chromatography. Purified proteins were formulated individually (A27L or L1R) and in combination (A27L and L1R) with 10% (w/w) Montanide™ Gel 01 PR adjuvant at a final antigen dose of 20 µg per protein. The safety and immunogenicity of these formulations were evaluated in rabbits in a 42-day clinical trial. Animals were vaccinated on day 0 and boost injection administered 21 days later. No reduced morbidity, increased temperature and any other clinical signs were recorded in vaccinated animals for all three vaccine formulations. The highest neutralizing antibody response was detected on day 42 post-primary vaccination for all formulations when using serum neutralising assay. The neutralisation data correlates with antibody titres quantified using a whole cell ELISA. Evaluating the combination of A27L and L1R as potential diagnostic reagents showed highest sensitivity for detection of antibodies against LSD when compared to individual proteins. This study reports the immunogenicity of recombinant A27L and L1R combination for successful application in LSD vaccine development. Furthermore, these proteins demonstrated the potential use in LSD diagnostics.

Students' challenging behavior is impacting negatively on teachers' wellbeing worldwide. Currently, teaching for some teachers in South African primary schools has become exhausting and daunting to say the least. Teachers feel that they have had enough degradation and are not receiving the respect they, as professionals, deserve. Students' relentless rampages and their refusal to listen to teachers are pushing teachers to the near brink of mental breakdown. This article, which embraces a qualitative research design and draws on an interpretative approach, derives from a larger case study conducted at three primary schools in Phoenix. To determine "how does students' challenging behavior impact on teachers," semi-structured interviews were conducted with five teachers from each sample school (N = 15). Thematic analysis was used to classify data for this study. The systems theory applied portrays how behavior transferred from the home and environmental system, into the school system was impacting on teachers. Results indicate that teachers are unhappy and are awaiting their exit from the teaching profession. Furthermore, lack of collaboration between systems deters any amalgamated progress. Future research should concentrate on embracing constructive systems to reduce teachers' mental and physical stresses in South African primary schools.

Nanotechnology has emerged as an effective means of removing contaminants from water. Traditional techniques for producing nanoparticles, such as physical methods (condensation and evaporation) and chemical methods (oxidation and reduction), have demonstrated high efficiency. However, these methods come with certain drawbacks, including the significant energy requirement and the use of costly and hazardous chemicals that may cause nanoparticles to adhere to surfaces. To address these limitations, researchers are actively developing alternative procedures that are cost-effective, environmentally safe, and user-friendly. One promising approach involves biological synthesis, which utilizes plants or microorganisms as reducing and capping agents. This review discusses various methods of nanoparticle synthesis, with a focus on biological synthesis using naturally occurring bioflocculants from microorganisms. Bioflocculants offer several advantages, including harmlessness, biodegradability, and minimal secondary pollution. Furthermore, the review covers the characterization of synthesized nanoparticles, their antimicrobial activity, and cytotoxicity. Additionally, it explores the utilization of these NPs in water purification and dye removal processes.

When the government in South Africa announced restrictions on the number of people that could meet in the wake of COVID-19, churches started implementing those measures in order to comply. This happened during the build-up to Easter celebrations. Even over the radio, Christians started to pronounce measures that would address such restrictions during Easter celebrations. Those pronouncements carried with them insinuations on how even the government should not encroach on the churches’ mandate to hold these celebrations. As it turned out, the restrictions were short-lived as they were immediately followed by lockdown. The rhetoric of criticising the government’s encroachment to the territory that belonged to the church was replaced by silent obedience. Faceless meetings erupted as congregants were organised into virtual groups complete with leaders and followers. At the same time, the criticism that had been placed on congregants who attended church on television ceased as many graduated from that tendency into attending church on their mobile phones. But this came with mixed responses as not all congregants had the necessary internet connectivity. This paper interrogates the tension between obeying the voice of God and the voice of the State. This is looked through two congregations: one in the ultra-urban uMhlanga area in Durban, KwaZulu-Natal Province and the other in the town of Empangeni in KwaZulu-Natal. The methodology employed in the paper is ethnography where the two churches are presented in the tradition of ethnographic thick description.

Abstract Background Lay health workers (LHWs) can support the HIV response by bridging gaps in human resources for health. Innovative strategies are needed to expand LHW programs in many low- and middle-income countries. Youth Health Africa (YHA) is a novel LHW approach implemented in South Africa that places young adults needing work experience in one-year non-clinical internships at health facilities to support HIV programs (e.g., as HIV testers, data clerks). While research suggests YHA can increase HIV service delivery, we need to understand healthcare worker perceptions to know if this is an acceptable and appropriate approach to strengthen human resources for health and healthcare delivery. Methods We conducted a convergent mixed methods study to assess healthcare worker acceptance and perceived appropriateness of YHA as implemented in Gauteng and North West provinces, South Africa and identify issues promoting or hindering high acceptability and perceived appropriateness. To do this, we adapted the Johns Hopkins Measure of Acceptability and Appropriateness to survey healthcare workers who supervised interns, which we analyzed descriptively. In parallel, we interviewed frontline healthcare workers who worked alongside YHA interns and conducted an inductive, thematic analysis. We merged quantitative and qualitative results using the Theoretical Framework of Acceptability to understand what promotes or hinders high acceptance and appropriateness of YHA. Results Sixty intern supervisors responded to the survey (91% response rate), reporting an average score of 3.5 for acceptability and 3.6 for appropriateness, on a four-point scale. Almost all 33 frontline healthcare workers interviewed reported the program to be highly acceptable and appropriate. Perceptions that YHA was mutually beneficial, easy to integrate into facilities, and helped facilities be more successful promoted a strong sense of acceptability/appropriateness amongst healthcare workers, but this was tempered by the burden of training interns and limited program communication. Overall, healthcare workers were drawn to the altruistic nature of YHA. Conclusion Healthcare workers in South Africa believed YHA was an acceptable and appropriate LHW program to support HIV service delivery because its benefits outweighed its costs. This may be an effective, innovative approach to strengthen human resources for HIV services and the broader health sector.

A matrix of poly (vinyl alcohol) (PVA) slime phantoms was investigated to replicate the published optical properties of the lung carcinoma and tumorous brain tissues at 630 nm wavelength. The effective attenuation coefficients (µeff) and penetration depths of light in the tissue phantoms were deduced from measurements of its optical properties and the anisotropy factor (g), obtained using the Henyey-Greenstein (H-G) phase function and Wolfram Mathematica. The mechanical response of the fabricated phantom materials was characterized and found to be flexible and mouldable into shapes and sizes comparable to desired tissue types.In the context of accuracy and stability, the phantoms were monitored for over two months and managed to preserve their optical properties with inconsiderable changes, possibly due to dehydration. Further data analysis is required to clarify if the optical properties would significantly change for long periods. We conclude that the fabrication of deformable tissue phantoms capable of uniformly incorporating any inserted lesion-like objects with relatively available materials and clear procedure is realizable. These phantoms are promising for testing and calibrating new optical imaging systems and developing disease diagnosis and treatment techniques.

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The effectiveness of optical methods such as photodynamic therapy (PDT) depends on the amount of light distribution within the tissue to aid their potential for early cancer detection in a quantitative and non-invasive manner. Knowledge of the effective attenuation coefficient and penetration depth for the laser light is crucial to ensuring that the tumour tissue receives adequate optical energy. This study investigated the effective attenuation coefficient and penetration depth of HeâNe 630 nm red laser light in polyvinyl alcohol slime glue phantoms simulating human brain tumour tissues. The effective attenuation coefficient (µeff) and penetration depth (δ) were deduced from the absorption coefficient (µa), scattering coefficient (µs), and anisotropy factor (g) obtained from the HenyeyâGreenstein (HâG) function by collimated laser beam measurements. We found that the effective attenuation coefficient and penetration depth were 0.25 ± 0.02 mmâ1 and 4.00 mm, respectively, in the simulated phantoms. These values were in reasonable agreement with values reported for malignant human brain tumour tissues in the literature. The constructed phantoms would be an excellent tool for the continued evaluation of PDT as an essential therapeutic procedure in cancer management.

Abstract Background Correctional settings in South Africa have disproportionately high rates of HIV infection; a large number of inmates living with HIV return to the community each year. The transition community adherence club (TCAC) intervention was a differentiated care delivery approach with structural and peer components designed to increase antiretroviral therapy (ART) adherence and HIV care engagement following release from incarceration. The objective of this study was to assess the acceptability of the TCAC intervention among HIV-infected community re-entrants to inform program revisions and future intervention designs. Methods This was a qualitative study set within a randomized controlled trial (RCT) of the TCAC intervention in South Africa. We conducted semi-structured, in-depth interviews with 16 re-entrants living with HIV and assigned to the intervention arm. All interviews were audio-recorded, transcribed, translated, and de-identified. Transcripts were coded and analyzed using content analysis, and acceptability was assessed using the Theoretical Framework of Acceptability (TFA). Results Overall, study participants reported that the TCAC intervention was acceptable. Development of supportive relationships between participants, non-judgmental attitudes from peer-facilitators, and perceived effectiveness of the intervention to support ART adherence and HIV care were noted as the most valued components. An altruistic desire to help other participants facing similar post-incarceration and HIV-related challenges was a key motivator for TCAC attendance. A lack of access to reliable transportation to intervention sites and clinic-based medication collection were described as burdens to program participation. Illicit drug use by other group members and negative social influences were also identified as potential barriers to optimal program engagement. Conclusion The TCAC was a well-accepted model of differentiated care delivery among re-entrants living with HIV in South Africa. To further enhance intervention acceptability for future scale-ups, program revisions should address logistical barriers related to reaching TCAC sites and implementing ART distribution at TCAC group sessions.

Young child malnutrition and food insecurity is the result of many factors including social and economic inequities, disempowerment of women, inadequate support for breastfeeding, degraded environments, poor sanitation, unsafe water, violence and conflict. In this complex context, humanitarian agencies have a responsibility to guard against unintended consequences and ensure that the promotion of “quick fix” interventions do no harm. One such intervention is the Small Quantity Lipid Nutrition Supplement (SQ-LNS), that UNICEF is recommending for introduction into national nutrition programs to reduce mortality. The authors believe this recommendation is based on questionable evidence. They point to a fundamental flaw that trials compared “an intervention (SQ-LNS)” with “no comparable food-based intervention.” In effect, a pre-determined outcome. Also present is a conflict of interest, with support and intellectual input coming from interested food companies. Many other concerns are listed: SQ-LNS is an ultra-processed food (UPF), as defined by Nova Classification; daily feeding to children with poor diets will not improve dietary diversity and may undermine confidence in more culturally appropriate and nutritious family foods; the double burden of malnutrition and the emerging pandemic of overnutrition; the environmental impact of packaging and most importantly the clear potential for commercial exploitation. The widespread use of SQ-LNS in the face of food poverty is viewed as an unsustainable, unsafe and nutritionally inappropriate response. UN and humanitarian agencies should use their considerable diplomatic influence to challenge corporate-led food systems and support governments in their efforts to protect, promote and support breastfeeding and the provision of diverse complementary foods.

Abstract Background South Africa has made significant progress in scaling up antiretroviral therapy (ART) to achieve the aspirational goal of HIV epidemic control. The aim of this study was to determine the prevalence of HIV, assess progress towards each of the Joint United Nations Programme on HIV/AIDS (UNAIDS) indicators and determine factors associated with achieving viral suppression among pregnant adolescents and women living with HIV in rural KwaZulu-Natal, South Africa. Methods Pregnant adolescents and women, 12 years and older seeking antenatal care at six primary health care clinics were enrolled in a cross-sectional study. Following written informed consent, structured questionnaires were administered, and finger-prick blood samples were collected for HIV antibody testing and viral load measurement. Viral suppression was defined as HIV viral load of

At the emergence of democracy in South Africa the government corrected linguistic imbalances by officialising eleven languages. Prior to that only English and Afrikaans were the recognised official languages. The Black population had rejected the imposition of Afrikaans as the medium of instruction. However, such rejection did not mean the adoption of indigenous languages as media of instruction; instead English was supposedly adopted as a unifying language among linguistically diverse Africans. Such implicit adoption of the English language has created a stalemate situation in the development of African languages to the level of English and Afrikaans. Although there is a widespread desire to promote indigenous languages to the level of being media of instruction, the desire is peripheral and does not carry the urgency that characterised the deposition of Afrikaans in the 1976 uprisings. On the other hand this paper argues that the hegemony of English language as a colonial instrument carries ambivalence in the minds of Black South Africans. Through ethnographic thick description of two learners, this hegemony is illustrated by the "kind" of English provided to most Black South African learners who do not have financial resources to access the English offered in former Model C schools. The paper concludes that Black South Africans do not only need urgency in the promotion and development of indigenous languages, but further need to problematize, in addition to the implicit adoption of English language, the quality of the language they have opted. The paper therefore suggests that this is possible through a decolonised mindset.

Studying the production of Iron (Fe) nanoparticles using natural substances is an intriguing area of research in nanotechnology, as these nanoparticles possess biocompatibility and natural stability, which make them useful for a variety of industrial applications. The study utilized Fe nanoparticles that were synthesized using a bioflocculant and applied to eliminate different kinds of pollutants and dyes found in wastewater and solutions. The study involved the generation of Fe nanoparticles through a bioflocculant obtained from Pichia kudriavzevii, which were evaluated for their flocculation and antimicrobial capabilities. The impact of the Fe nanoparticles on human embryonic kidney (HEK 293) cell lines was studied to assess their potential cytotoxicity effects. An array of spectroscopic and microscopic methods was employed to characterize the biosynthesized Fe nanoparticles, including SEM-EDX, FT-IR, TEM, XRD, UV-vis, and TGA. A highly efficient flocculating activity of 85% was achieved with 0.6 mg/mL dosage of Fe nanoparticles. The biosynthesized Fe nanoparticles demonstrated a noteworthy concentration-dependent cytotoxicity effect on HEK 293 cell lines with the highest concentration used resulting in 34% cell survival. The Fe nanoparticles exhibited strong antimicrobial properties against a variety of evaluated Gram-positive and Gram-negative microorganisms. The efficiency of removing dyes by the nanoparticles was found to be higher than 65% for the tested dyes, with the highest being 93% for safranine. The Fe nanoparticles demonstrated remarkable efficiency in removing various pollutants from wastewater. In comparison to traditional flocculants and the bioflocculant, biosynthesized Fe nanoparticles possess significant potential for eliminating both biological oxygen demand (BOD) and chemical oxygen demand (COD) from wastewater samples treated. Hence, the Fe nanoparticles synthesized in this way have the potential to substitute chemical flocculants in the treatment of wastewater.

Abstract Background HIV testing is the entry point into the HIV care continuum and critical for HIV epidemic control. Facility-based HIV testing services (HTS) reach individuals who are already seeking clinical care and engaging with the medical care system. For this reason, individuals diagnosed with HIV during facility-based HIV testing are more likely to continue into HIV care. To increase the number of PLHIV who are diagnosed and initiated on ART, in 2015, the South African Department of Health instituted Provider-Initiated Counselling and Testing (PICT) policy—encouraging healthcare providers to recommend HIV testing, but this strategy remains under-utilized. We aimed to identify key constraints to the normalization of PICT implementation in 10 Ekurhuleni District healthcare facilities in South Africa. Methods In-depth interviews were conducted with 40 healthcare workers (28 clinicians and 12 lay counsellors). Health care workers were purposefully selected to participate in the interviews, stratified by health facility and work category. Interviews were audio-recorded, transcribed, and translated for analysis. Thematic analysis was guided by the normalization process theory (NPT). NPT theory explains how practices are routinely embedded within organizational contexts. We used NVivo 10 software for qualitative data management. Results Both clinicians and lay counsellors exhibited a clear understanding of the PICT policy— acknowledging its purpose and value. The identified barrier to normalization of PICT among clinicians was offering HIV testing based on suspicion of HIV despite understanding that PICT involves offering testing to all clients. Additionally, clinicians perceived PICT as incongruent with their clinical roles and perceived it to be lay counsellors’ responsibility. The main facilitator was the participation of all healthcare workers, specifically the presence of lay counsellors, although they also faced barriers such as a lack of workspace and under-appreciation. Conclusions Use of NPT helped identify barriers that prevent the normalization of PITC and its integration into routine patient care. These barriers can be modified by low-cost interventions that promote congruence of PICT to the roles of clinicians and integrate the role of lay counsellors within the patient flow in the facility.

The global spread and dominance of English in higher education has reached alarming heights. While there has been a drive to argue for the relevance and importance of education through local languages, English has snatched the biggest slice of the cake by subtly imposing itself as the sole global language of education. This paper interrogates the sociolinguistic problems posed by the hegemony of English language. It argues that globalization and internationalization work in tandem with neo-colonial and neoliberal operations to create a class of global citizens that must support the economic aspirations of English imperial expansion and sustenance. The arguments are drawn from the experiences of Middle East and North Africa as well as lessons from Eastern and Southern Africa. The paper adopts a critical approach in order to place urgency against the onslaught of English medium of instruction in global higher education. This is done by problematizing the rhetoric of globalized and internationalized education. The paper then draws conclusions on epistemic access in the context of burgeoning knowledge economies. It argues that English medium of instruction is implicated in stalling access to knowledge for the great majority in order to cater for and safeguard the economic dominance of the elite minority.

Abstract Objective The proliferation of false information on COVID-19 mostly through social media is adversely affecting control efforts. The objective of this study was to identify areas where targeted effective messaging can be useful in demystifying misinformation against COVID-19. Results The study showed high levels of misinformation on COVID-19 in the study area [mean score 2.71; standard deviation (SD) 1.5]. The highest levels of misinformation were observed in Dr. Ruth Segomotsi Mompati district, North West province (mean score: 3.84; SD: 2.1) and Sedibeng district, Gauteng province (mean score: 3.56; SD 1.7). Higher levels of misinformation were reported by those aged 18–24 years (mean score: 3.48; SD: 1.8), and men (mean score: 2.73; SD: 1.8). Across the two provinces, we identified geospatial hot and coldspots of misinformation highlighting the need to implement point of care strategies such as targeted messaging. Findings showed the need for targeted interventions to young people, students, those with low levels of education and the self-employed in the two districts more importantly, as South Africa expands its nationwide vaccination roll-out.

Biolocculants are gaining attention in research due to their environmental friendliness and innocuousness to human in comparison to the conventional flocculants. The present study aimed to investigate the ability of fungi from Kombucha tea SCOBY to produce effective bioflocculant in bulk. A 16S rRNA gene sequence analysis was utilized to identify the isolate. The medium composition (carbon and nitrogen sources) and culture conditions (inoculum size, temperature, shaking speed, pH, and time) were optimized using one-factor-at-a-time method. The functional groups, morphology, and crystallinity of the bioflocculant were evaluated using Fourier transform infrared (FT-IR), scan electron microscope (SEM) and X-ray diffractometry (XRD). The fungus was found to be Pichia kudriavzevii MH545928.1. It produced a bioflocculant with flocculating activity of 99.1% under optimum conditions; 1% (v/v) inoculum size, glucose and peptone as nutrient sources, 35 °C, pH 7 and the shaking speed of 140 rpm for 60 h. A cumulus-like structure was revealed by SEM; FT-IR displayed the presence of hydroxyl, carboxyl, amine, and thiocynates. The XRD analysis demonstrated the bioflocculant to have big particles with diffraction peaks at 10° and 40° indicating its crystallinity. Based on the obtained results, P. kudriavzevii MH545928.1 has potential industrial applicability as a bioflocculant producer.

The Lumpy Skin Disease Virus (LSDV) Neethling vaccine strains have been used for decades for prophylactic immunization of domestic ruminants against the disease. Commercial products against Lumpy skin disease are supplied as live attenuated vaccines and often are associated with adverse reactions warranting studies towards development of safe and efficacious vaccine alternatives. The present study was designed to investigate the ability of Montanide™ Gel 01 PR adjuvanted inactivated Neethling vaccine strain of the lumpy skin disease to induce immune response in rabbits. Complete virus inactivation was achieved following treatment of live vaccine strain with binary ethyleneimine (BEI) at 2 mM final concentration. Inactivated virus antigen, formulated with Montanide™ Gel 01 was injected at 1,00E + 05 and 1,00E + 06 TCID50 per dose in rabbits. The second injection with same vaccine dosages was administered 21 days after the primary vaccination. Rabbits that received a 1,00E + 05 TCID50/dose of inactivated LSDV vaccine formulation induced maximum neutralizing antibody titres on day 13 post second vaccinations. Rabbits vaccinated and prime boosted with the 1,00E + 06 TCID50/dose of inactivated LSDV vaccine formulation, induced neutralizing antibody titres on day 14 after first vaccination. The maximum antibody titres for the 1,00E + 06 TCID50/dose of the inactivated LSDV vaccine formulation was obtained on day 35 post vaccination. The 1,00E + 06 TCID50 dose of the inactivated LSDV vaccine Montanide™ Gel-01 PR formulation induced higher neutralizing antibodies. The MontanideTM Gel-01 PR offers safer profile to oil adjuvants and can be developed further to protect target animals against LSDV in non-endemic areas.

South Africa has been battling with getting land reform right since the advent of democratic rule in 1994 The need to expedite land reform in the country has given rise to a number of radical approaches, including the topic and controversial expropriation of land without compensation discourse. The simulation results presented in this paper provide nuanced policy options for land redistribution in South Africa in the face of the looming expropriation of land without compensation. The analysis in this paper is specific to agricultural land as opposed to land in general. We carried out scenario simulation through Computable General Equilibrium approach using the modified University of Pretoria General Equilibrium Model (UPGEM), which is solved using GEMPACK solution software. Our simulation revealed that there would be adjustment costs regardless of the option(s) chosen. The Inclusive Scenario came up as the most suitable policy option in terms of minimal adjustment costs and allowing the sector to continue to grow, albeit at a lower rate compared to the status quo. Given that agricultural land reform is imperative in South Africa, policy makers should opt for the policy option that is least disruptive to the economy while achieving the desired outcome of more equitable agricultural land ownership and broader participation in the agricultural sector by the majority in the country. Thus, the Inclusive Scenario was found to be the most suitable policy option for agriculture land reform in South Africa.

This paper presents prospective of using solar energy resources to produce hydrogen for feeding hydrogen vehicles at a refuelling station in South Africa. Solar energy sources from Vredendal, located in the Western Cape Province of South Africa, were used for the study. Assessment was performed for a refuelling station having capacity to meet the hydrogen needs of 25, 50 and 100 number of vehicles per day. The results showed that the annual daily average solar irradiation on the horizontal surfaces and optimally tilted surfaces were 5.77 kWh/m2 and 5.933 kWh/m2, respectively. The solar power required to meet the hydrogen production demand for each of the three refuelling number of vehicles were determined to be 2.24, 4.24 and 8.95 MW, respectively. The cost of energy from the solar PV system as well as the cost of hydrogen production at the refuelling station for each of the three hydrogen vehicle capacities was calculated as (0.24, 0.239 and 0.237) $/kWh and (16.52, 15.95 and 15.67) $/kg, respectively. The PV system would displace 387, 774 and 1548 tons of coal per annum for each of the capacity, respectively. This will result in the avoidance of 780, 1560 and 3120 tons/yr of CO2, respectively.

In an attempt to standardise Foundation Programmes for Oman higher education providers, the Oman Academic Standards for General Foundation Programmes stipulated that higher education providers should offer programmes that ensure androgogic effectiveness. In the light of that, this paper presents attempts by a University College in Oman to introduce project based learning through a 'Project' component offered at Foundation level. However, in establishing the androgogical learning theory it became necessary to consider the seemingly inflated false dichotomy between pedagogy and androgogy by way of highlighting learning theories that underpin project based learning. Furthermore, the paper shows how skills that are thought to be transferable from "Project" to other components fall short due to "Project" being a subject on its own rather than an approach which compromises the envisaged androgogic effectiveness. Nonetheless, benefits of "Project" as a subject component are presented in the form of soft skills which are mainly the expected learning outcomes. Therefore, the paper proposes the implementation of project based learning in ensuring pedagogical relevance and androgogic effectiveness among higher education providers and further demonstrates the extent at which this is realised in the university college where the study was conducted followed by recommendations based on the findings of this study.

Power grids of the future will likely incorporate more renewable energy distributed generation (REDG), also known as alternative energy systems. REDG units are increasingly being used in electrical transmission networks because of the positive effects they have on power networks. REDG systems are the backbone of smart electric networks and are essential to the operation of the smart grid. These REDG systems can additionally improve system reliability by providing some customers with a backup generator in the event of power interruptions. This review offers a thorough evaluation of the existing body of information on the topic of electric vehicles’ (EVs’) future interactions with smart grids. The combination of the potential deployment of EVs and the smart grid’s conceptual goal presents challenges for electric grid-related infra-structure, communication, and control. The proposal for connecting EVs to the grid is based on research into cutting-edge smart metering and communication systems. In the context of the vehicle-to-grid (V2G) phenomenon, the possibilities, benefits, and limitations of various EV smart-charging systems are also fully examined. A quickly growing percentage of distributed energy is derived from wind and solar (photovoltaic) energy. The variable power output of wind and solar energy introduces fresh challenges for those responsible for organizing, operating, and controlling the power grid. While fluctuations in the electric grid are problematic, they may be mitigated by the entry of EVs into the energy market. As such, we performed a comprehensive review of the literature to learn more about this exciting research gap that needs to be filled and to identify recently developed solutions to the problems related to EVs. Additionally, in this review article, we take a close look at the practicality of V2G technology. The smart grid is a developing concept that will likely have large implications for the world’s energy infrastructure, and this study thoroughly analyzes how EVs interact with it.

Electric vehicles (EVs) are gaining more and more traction as a viable option in the automotive sector. This mode of transportation is currently on track, according to current trends, to totally replace internal combustion engine (ICE) cars in the not-too-distant future. The economic system, the energy infrastructure, and the environment are just a few of the areas where electric vehicles could have a major impact. The transportation industry produces the second-most carbon dioxide gas from the combustion of fossil fuels, making it the second-highest contributor to global warming. A lot of people are looking to EVs, or electric vehicles, as a possible game-changing answer to this problem. Since an electric motor drives the electric vehicle’s propeller instead of an internal combustion engine, electric vehicles can reduce their carbon dioxide (CO2) emissions compared to traditional automobiles. If coupled with renewable energy sources, EVs might theoretically become emission-free automobiles. In this paper, we will examine the various EV drive circuit types, including their construction and the benefits and drawbacks of employing each. This article discusses the current state of battery technology with an emphasis on EV batteries. This article discusses the best electric motor for EVs in terms of efficiency, power density, fault tolerance, dependability, cost, and more. Next, we conduct in-depth research into the difficulties and potential rewards of EV adoption in the future. While improvements in areas like charging times and battery performance are encouraging, government regulation of EVs remains a big non-technical barrier.

People, unlike most animals are born vulnerable, and they need to be nurtured and raised in a safe environment to thrive and survive. Human development is holistic and complicated. Much of development is dependent on learning through senses such as sight, hearing, touch, taste, and smell. People need all these senses to learn the world around them. Worldwide about 253 million people have visual impairment (VI) problem (WHO, 2018). Depending on the type of visual problem (congenital or adventitious), VI makes life difficult for people infected compared to sighted people. People with VI face challenges such as unemployment, injuries through falling and accidents, low-self-esteem, isolation, depression, and difficulty in mobility within communities. The purpose of this literature study was to congregate relevant information on VI and coping strategies in South Africa. Articles reviewed were identified through search engines such as JSTOR, Google Scholar, Boloka-NWU Institutional Repository (NWUIR), ProQuest, EBSCOHost, Scopus, Science Direct and Web of Science were the databases and search engines used in the search. The data collected was then further presented and analysed using Atlas Ti-version 8.4.23 software. The results of this literature study found that people with VI face psychological challenges such as depression, low self-esteem, loss of self-worth and emotional distress. The results further point out that physical challenges are also experienced such as frequent falls, lack of societal resources, inability to perform daily living activities such as cooking, and transportation and mobility. Lastly people with VI also experienced social challenges and environmental challenges such unemployment, unable to use technology and poor quality of life. To cope with challenges, people with VI utilize strategies such strategic planning, meaning making, engage in mind stimulating activities, avoidance coping, substance abuse, family acceptance and optimism.

Background: Uncorrected refractive error is one of the leading causes of visual impairment. Understanding of the barriers to spectacle usage and attitudes of students towards spectacle use and management strategies can help improve population knowledge and develop school-based programmes to address refractive error. Aim: This study aimed to determine the knowledge, attitudes and practices of university students towards refractive error. Setting: A large university in South Africa. Methods: A quantitative descriptive study was performed via a validated self-administered questionnaire. The survey included questions regarding the knowledge of the participants current status of vision, knowledge on causes of poor vision, accessibility of eye healthcare facilities and attitudes towards spectacle use. Results: Knowledge of participants with respect to the cause of poor vision amongst their peers revealed 63% (n = 189) reporting that digital devices are the major cause of poor vision amongst students, followed by myopia (n = 166) and lastly long-sightedness (n = 117). The majority of participants had adequate knowledge regarding methods of correcting poor vision with 95% listing spectacles as a method of correction followed by contact lenses (n = 250) and surgery (n = 203). There were 29% of participants who reported using spectacles all the time and 22% who reported using spectacles some of the time. Conclusion: The university students are knowledgeable about the causes of poor vision amongst students. Some students have refractive error but display poor attitude and practice towards spectacle wear because they do not wear spectacles as often as they should. Awareness around eye care and spectacle use must be created.

Students' challenging behavior has become a serious problem internationally. In South Africa, there are reports highlighting that even primary schools experience serious forms of challenging behavior by students. This article emanates from a case study that utilized the social cognitive theory to understand how primary schools address this phenomenon. To get students' perspectives of what causes them to exhibit challenging behavior, a purposive sample of nine students (N = 9) from three primary schools was identified. Data were generated through face-to-face and focus group interviews. Findings show that there are more school than home factors impacting primary school students' lives and influencing behavior. The article concludes that primary school teachers need to understand the causative factors of challenging behaviors and consider changing school cultures as a possible solution to school improvement efforts. Further research should include a larger sample of schools and multicultural students to shed more light on this phenomenon.

Due to their broad-spectrum activity against Gram-negative and Gram-positive bacteria, natural antimicrobial peptides (AMPs) and their synthetic analogs have emerged as prospective therapies for treating illnesses brought on by multi-drug resistant pathogens. To overcome the limitations of AMPs, such as protease degradation, oligo-N-substituted glycines (peptoids) are a promising alternative. Despite having the same backbone atom sequence as natural peptides, peptoid structures are more stable because, unlike AMP, their functional side chains are attached to the backbone nitrogen (N)-atom rather than the alpha carbon atom. As a result, peptoid structures are less susceptible to proteolysis and enzymatic degradation. The advantages of AMPs, such as hydrophobicity, cationic character, and amphipathicity, are mimicked by peptoids. Furthermore, structure-activity relationship studies (SAR) have shown that tuning the structure of peptoids is a crucial step in developing effective antimicrobials.

Virulent Enterobacterale strains expressing serine and metallo-β-lactamases (MBL) genes have emerged responsible for conferring resistance to hard-to-treat infectious diseases. One strategy that exists is to develop β-lactamase inhibitors to counter this resistance. Currently, serine β-lactamase inhibitors (SBLIs) are in therapeutic use. However, an urgent global need for clinical metallo-β-lactamase inhibitors (MBLIs) has become dire. To address this problem, this study evaluated BP2, a novel beta-lactam-derived β-lactamase inhibitor, co-administered with meropenem. According to the antimicrobial susceptibility results, BP2 potentiates the synergistic activity of meropenem to a minimum inhibitory concentration (MIC) of ≤1 mg/L. In addition, BP2 is bactericidal over 24 h and safe to administer at the selected concentrations. Enzyme inhibition kinetics showed that BP2 had an apparent inhibitory constant (Kiapp) of 35.3 µM and 30.9 µM against New Delhi Metallo-β-lactamase (NDM-1) and Verona Integron-encoded Metallo-β-lactamase (VIM-2), respectively. BP2 did not interact with glyoxylase II enzyme up to 500 µM, indicating specific (MBL) binding. In a murine infection model, BP2 co-administered with meropenem was efficacious, observed by the >3 log10 reduction in K. pneumoniae NDM cfu/thigh. Given the promising pre-clinical results, BP2 is a suitable candidate for further research and development as an (MBLI).

The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for COVID-19, which was declared a global pandemic in March 2020 by the World Health Organization (WHO). Since SARS-CoV-2 main protease plays an essential role in the virus’s life cycle, the design of small drug molecules with lower molecular weight has been a promising development targeting its inhibition. Herein, we evaluated the novel peptidomimetic azatripeptide and azatetrapeptide nitriles against SARS-CoV-2 main protease. We employed molecular dynamics (MD) simulations to elucidate the selected compounds’ binding free energy profiles against SARS-CoV-2 and further unveil the residues responsible for the drug-binding properties. Compound 8 exhibited the highest binding free energy of −49.37 ± 0.15 kcal/mol, followed by compound 7 (−39.83 ± 0.19 kcal/mol), while compound 17 showed the lowest binding free energy (−23.54 ± 0.19 kcal/mol). In addition, the absorption, distribution, metabolism, and excretion (ADME) assessment was performed and revealed that only compound 17 met the drug-likeness parameters and exhibited high pharmacokinetics to inhibit CYP1A2, CYP2C19, and CYP2C9 with better absorption potential and blood-brain barrier permeability (BBB) index. The additional intermolecular evaluations suggested compound 8 as a promising drug candidate for inhibiting SARS-CoV-2 Mpro. The substitution of isopropane in compound 7 with an aromatic benzene ring in compound 8 significantly enhanced the drug’s ability to bind better at the active site of the SARS-CoV-2 Mpro.