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Sarka Salajkova,1,2 Filip Havel,1,3 Michal Sramek,1 Filip Novotny,1,4 David Malinak,2,5 Rafael Dolezal,2,5 Lukas Prchal,2 Marketa Benkova,2 Ondrej Soukup,2 Kamil Musilek,2,5 Kamil Kuca,2,5 Jiri Bartek,1,6,7 Jan Proska,3 Monika Zarska,1 Zdenek Hodny1 1Department of Genome Integrity, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic; 2Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic; 3Department of Physical Electronics, Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University in Prague, Prague, Czech Republic; 4Center for Advanced Functional Nanorobots, Department of Inorganic Chemistry, Faculty of Chemical Technology, University of Chemistry and Technology Prague, Prague, Czech Republic; 5Department of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove, Czech Republic; 6Genome Integrity Unit, Danish Cancer Society Research Center, Copenhagen, Denmark; 7Department of Medical Biochemistry and Biophysics, Science for Life Laboratory, Division of Genome Biology, Karolinska Institute, Stockholm, SwedenCorrespondence: Monika ZarskaDepartment of Genome Integrity, Institute of Molecular Genetics of the Czech Academy of Sciences, Videnska 1083, Prague 4, Prague, CZ 142 20, Czech RepublicTel +420- 24106 3151Fax + 420-24106 2289Email monika.zarska@img.cas.czKamil KucaBiomedical Research Center, University Hospital Hradec Kralove, Sokolska 581, Hradec Kralove, CZ 500 05, Czech RepublicTel +420-495 833 447Email kamil.kuca@fnhk.czPurpose: Plasmonic photothermal cancer therapy by gold nanorods (GNRs) emerges as a promising tool for cancer treatment. The goal of this study was to design cationic oligoethylene glycol (OEG) compounds varying in hydrophobicity and molecular electrostatic potential as ligand shells of GNRs. Three series of ligands with different length of OEG chain (ethylene glycol units = 3, 4, 5) and variants of quaternary ammonium salts (QAS) as terminal functional group were synthesized and compared to a prototypical quaternary ammonium ligand with alkyl chain – (16-mercaptohexadecyl)trimethylammonium bromide (MTAB).Methods: Step-by-step research approach starting with the preparation of compounds characterized by NMR and HRMS spectra, GNRs ligand exchange evaluation through characterization of cytotoxicity and GNRs cellular uptake was used. A method quantifying the reshaping of GNRs was applied to determine the effect of ligand structure on the heat transport from GNRs under fs-laser irradiation.Results: Fourteen out of 18 synthesized OEG compounds successfully stabilized GNRs in the water. The colloidal stability of prepared GNRs in the cell culture medium decreased with the number of OEG units. In contrast, the cellular uptake of OEG+GNRs by HeLa cells increased with the length of OEG chain while the structure of the QAS group showed a minor role. Compared to MTAB, more hydrophilic OEG compounds exhibited nearly two order of magnitude lower cytotoxicity in free state and provided efficient cellular uptake of GNRs close to the level of MTAB. Regarding photothermal properties, OEG compounds evoked the photothermal reshaping of GNRs at lower peak fluence (14.8 mJ/cm2) of femtosecond laser irradiation than the alkanethiol MTAB.Conclusion: OEG+GNRs appear to be optimal for clinical applications with systemic administration of NPs not-requiring irradiation at high laser intensity such as drug delivery and photothermal therapy inducing apoptosis.Keywords: gold nanorods, quaternary ammonium salts, oligoethylene glycol, cellular uptake, photothermal stability

The maximum ion energy and acceleration efficiency have to be increased for practical applications of intense ion beams produced by intense short laser pulses incident on a thin foil. For this aim, we propose to use foil with a microscopic structure on the front size. We have prepared such targets by depositing a monolayer of polystyrene nanospheres of a size comparable to laser wavelength on a thin foil. The damage threshold of the produced targets is found experimentally above 3.5 × 109 W/cm2 for a nanosecond pedestal and above 1011 W/cm2 for femtosecond prepulses.

Proton acceleration from the interaction of high contrast, 25 fs laser pulses at >1019 W/cm2 intensity with plastic foils covered with a single layer of regularly packed micro-spheres has been investigated experimentally. The proton cut-off energy has been measured as a function of the micro-sphere size and laser incidence angle for different substrate thickness, and for both P and S polarization. The presence of micro-spheres with a size comparable to the laser wavelength allows to increase the proton cut-off energy for both polarizations at small angles of incidence (10°). For large angles of incidence, however, proton energy enhancement with respect to flat targets is absent. Analysis of electron trajectories in particle-in-cell simulations highlights the role of the surface geometry in the heating of electrons. [ABSTRACT FROM AUTHOR]

Compact bone is the preferred study material for isotope and chemical analyses to reconstruct paleodiet and paleoenvironment. As discussed in the literature, the results of such analyses can be hindered due to diagenetic alterations caused by dissolving and recrystallization phenomena. Although post-mortem alterations of buried bone have been carefully studied in these last few years, they are still not very well understood. The aim of the present study is the quantification of microstructural features in light microscope images of histological cross sections of compact femur bone by using an image analysis software package (KS 300 ZEISS). The samples were taken from skeletal remains buried in three different sites in eastern Austria. They are characterized by different repository materials and originate from different culture periods (loess in the Neolithic site Schletz; river gravel in the Early Bronze Age site Franzhausen; humus in the Medieval site Gars). A total of 71 midshaft femur bone samples were investigated. The histological cross sections were analyzed for their collagen structures using polarized light and regions of putative microbial activities (appearing as dark spots with blurred boundaries). The microstructure changes thus determined differ considerably depending on the repository material and the bronze from grave goods (whenever present). These identified collagen features have been chemically analyzed so as to quantitatively determine the histological content. Financial support was granted by the Austrian Science Research Fund (P12840CHE).