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Anacroneuria froehlichi n. sp. Miguel, Almeida & Bispo (Figs. 11A–G) Material examined. BR, GO. Holotype male: Chapada dos Veadeiros, Alto Paraíso de Goiás, Ribeirão Couro, Cachoeira São Bento, 13.xii.2006, PCB (MZUSP). Paratypes: Chapada dos Veadeiros, Alto Paraíso de Goiás, Ribeirão Saltador, 12.xii.2006, PCB, 7 males (CLBA); Ribeirão Saltador, 12.xii.2006, PCB, 8 males (MZUSP); Loquinhas, 15.xii.2006, PCB, 1 male (MZUSP); Ribeirão Água Fria, 14°05'28.8"S 47°29'40.9"W, 16.xii.2006, PCB, 1 male (MZUSP). Morphometric data. Holotype male, anterior wing length: 11.5 mm; paratype males (n= 17), anterior wing length: 11.5–16.0 mm. Description. General color yellowish to ochraceous. Head and pronotum ochraceous; head with inconspicuous M-line, central frons darker, clypeus and occiput yellowish (Fig. 11A). Antennae ochraceous, scape with darker spot laterally. Palpi pale yellowish. Pronotum with posterior corners rounded (Fig. 11A). Wings with membranes ochraceous and conspicuous veins. Presence of 4 to 8 transverse veins between the medial and anterior cubital veins. Legs yellowish with darker stripe in distal part of the femur. Abdomen pale yellowish. Sternum IX with small bristles on posterior margin; hammer as a short truncate cone (Fig. 11D). Penial armature, in dorsal view (Fig. 11E), tapering gradually up to the level of distal part of the hooks where there is a rounded expansion, which subsequently narrows sharply in an elongated final process; in ventral view (Fig. 11F), with pair of large distal vesicles and with hooks regularly curved with tips pointed; in the lateral view (Fig. 11G), with the apex slightly inclined ventrally. Cerci ochraceous. Remarks. Anacroneuria froehlichi was collected in the streams of Chapada dos Veadeiros, northern Goiás state. This species has a color pattern common to several species of the genus. A. froehlichi has penial armature (in dorsal view) with an abruptly narrowed apex, forming a slender process. This penial armature shape of A. froehlichi is unique, easily distinguishing it from other species of the genus. Etymology. The name honors Claudio Gilberto Froehlich, a pioneer in Plecoptera research in South America., Published as part of Miguel, Marina, Almeida, Lucas Henrique De, Novaes, Marcos Carneiro & Bispo, Pitágoras Da Conceição, 2022, Anacroneuria (Plecoptera: Perlidae) from Central Brazil: new species, new geographic records, and taxonomic notes, pp. 487-506 in Zootaxa 5162 (5) on page 502, DOI: 10.11646/zootaxa.5162.5.2, http://zenodo.org/record/6816755

Anacroneuria singularis Righi-Cavallaro & Lecci, 2010 (Figs. 9D–F) Anacroneuria singularis Righi-Cavallaro & Lecci, 2010: 43; Novaes & Bispo, 2014c: 435; Ribeiro et al., 2015: 295; Ribeiro & Gorayeb, 2016: 434; Firmino et al., 2019: 447; Rippel et al., 2019: 360; Carvalho et al., 2020: 439. Material examined. BR, GO. Parque Nacional das Emas, Chapadão do Céu, sede, prox. ao Rio Formoso; 28.iii - 06.iv.1984, SAV & DA, 2 males (CLBA). Pirenópolis, Córrego Vagafogo; 10.ix.1993, LGO, 1 male (CLBA); Rio das Almas; 10.x.1993, LGO, 2 males (CLBA); Rio das Almas; 14.xi.1993, LGO, 3 males (CLBA); Rio das Almas; 12.xii.1993, LGO, 2 males (CLBA); Rio das Almas; 13.iii. 1994, LGO, 5 males (CLBA); Rio das Almas; 14. i.1994, LGO, 2 males (CLBA); Rio das Almas; 03.ii.1994, LGO, 7 males (CLBA); Córrego Vagafogo; 04.ii.1994, LGO, 1 male (CLBA); Rio das Almas; 13.iii. 1994, LGO, 5 males (CLBA); 28.x.1994, HLRP, 1 male (CLBA); 29.x.1994, CL, 3 males (CLBA); Córrego Inferno; 13.i.1994. LGO, 1 male (CLBA); 29.x.1994, MVLV, 1 male (CLBA). Chapada dos Veadeiros, Alto Paraíso de Goiás, Ribeirão Couro, Cachoeira São Bento, 13.xii.2006, PCB, 1 male (CLBA). Morphometric data. Male (n=32), anterior wing length: 9.5–12.0 mm. Remarks. The species is widely distributed and was recorded in Minas Gerais, Mato Grosso do Sul, Pará, Rondônia, São Paulo, and Tocantins states. It is the first record in Goiás state. The species has a characteristic penial armature (Righi-Cavallaro & Lecci 2010), and the nymph was originally described by Ribeiro et al. (2015)., Published as part of Miguel, Marina, Almeida, Lucas Henrique De, Novaes, Marcos Carneiro & Bispo, Pitágoras Da Conceição, 2022, Anacroneuria (Plecoptera: Perlidae) from Central Brazil: new species, new geographic records, and taxonomic notes, pp. 487-506 in Zootaxa 5162 (5) on page 499, DOI: 10.11646/zootaxa.5162.5.2, http://zenodo.org/record/6816755, {"references":["Righi-Cavallaro, K. O. & Lecci, L. S. (2010) Three new species of Anacroneuria (Plecoptera: Perlidae) from Centre-West and Southeast Brazil. Zootaxa, 2683 (1), 35 - 44. https: // doi. org / 10.11646 / zootaxa. 2683.1.3","Novaes, M. C. & Bispo, P. C. (2014 c) Plecoptera from Minas Gerais State, southeastern Brazil. Zootaxa, 3856 (3), 433 - 442. https: // doi. org / 10.11646 / zootaxa. 3856.3.8","Ribeiro, J. M. F., Gorayeb, I. S. & Bispo, P. C. (2015) Description of the nymph of Anacroneuria singularis Righi-Cavallaro & Lecci (Plecoptera: Perlidae) and a new locality record for northern Brazil. Zootaxa, 4057 (2), 295 - 300. https: // doi. org / 10.11646 / zootaxa. 4057.2.11.","Ribeiro, J. M. F. & Gorayeb, I. S. (2016) Description of immatures associated with adults of two species of Macrogynoplax Enderlein and one species of Enderleina Jewett (Plecoptera: Perlidae) of the Brazilian Amazon. Zootaxa, 3957 (1), 69 - 76. https: // doi. org / 10.11646 / zootaxa. 3948.1.7","Firmino, V. C., Ribeiro, J. M. F., dos Santos, E. M. & Juen, L. (2019) First occurrence of Anacroneuria singularis Righi-Cavallaro & Lecci, 2010 (Plecoptera: Perlidae) in Rondonia, western Amazonia, Brazil. Zootaxa, 4544 (3), 446 - 450. https: // doi. org / 10.11646 / zootaxa. 4544.3.10","Rippel, M. L. S., Novaes, M. C. & Krolow, T. K. (2019) First records of genus Anacroneuria (Plecoptera: Perlidae) from Tocantins State, Brazil and description of a new species. Zootaxa, 4560 (2), 355 - 364. https: // doi. org / 10.11646 / zootaxa. 4560.2.8","Carvalho, A. S., Almeida, L. H., Azevedo, C. A. S. & Lima, L. R. (2020) The genus Anacroneuria Klapalek, 1909 (Plecoptera: Perlidae) from Maranhao State, northeastern Brazil. Zootaxa, 4860 (3), 435 - 44. https: // doi. org / 10.11646 / zootaxa. 4860.3.8"]}

Anacroneuria payagua Froehlich, 2007 (Figs. 9A–C) Anacroneuria payagua Froehlich, 2007: 22; Froehlich, 2010: 166; Righi-Cavallaro & Lecci, 2010: 36; Righi-Cavallaro & Froehlich, 2013: 579, Lecci & Righi-Cavallaro, 2017: 2. Material examined. BR, GO. Pirenópolis, Rio das Almas, 13.vii.1993, LGO, 1 male (CLBA); Rio das Almas, 09.ix.1993, LGO, 2 males (CLBA); Rio das Almas, 10.x.1993, LGO, 1 male (CLBA); Rio das Almas, x.1993, LGO, 1 male (CLBA); Rio das Almas, 14.xi.1993, LGO, 10 males (CLBA); Rio das Almas, 12.xii.1993, LGO, 11 males (CLBA); Rio das Almas, 14.i.1994, LGO, 5 males (CLBA); Rio das Almas, 13.iii. 1994, LGO, 1 male (CLBA); Rio das Almas, 10.iv.1994, LGO 1, male (CLBA). Morphometric data. Male (n=33), anterior wing length: 8.0–15.0 mm. Remarks. Anacroneuria payagua was originally described in Mato Grosso do Sul state (Froehlich 2007), and the present study now expands its distribution to Central Brazil. The species was collected at Serra dos Pireneus and this is a new record for Goiás state. The penial armature of A. payagua is quite characteristic and agrees with the original description (Froehlich 2007). The nymph was described by Righi-Cavallaro & Froehlich (2013)., Published as part of Miguel, Marina, Almeida, Lucas Henrique De, Novaes, Marcos Carneiro & Bispo, Pitágoras Da Conceição, 2022, Anacroneuria (Plecoptera: Perlidae) from Central Brazil: new species, new geographic records, and taxonomic notes, pp. 487-506 in Zootaxa 5162 (5) on page 499, DOI: 10.11646/zootaxa.5162.5.2, http://zenodo.org/record/6816755, {"references":["Froehlich, C. G. (2007) Three new species of Anacroneuria (Plecoptera: Perlidae) from State of Mato Grosso do Sul, Brazil. Zootaxa, 1461 (1), 15 - 24. https: // doi. org / 10.11646 / zootaxa. 1461.1.2","Froehlich, C. G. (2010) Catalogue of Neotropical Plecoptera. Illiesia, 6 (12), 118 - 205.","Righi-Cavallaro, K. O. & Lecci, L. S. (2010) Three new species of Anacroneuria (Plecoptera: Perlidae) from Centre-West and Southeast Brazil. Zootaxa, 2683 (1), 35 - 44. https: // doi. org / 10.11646 / zootaxa. 2683.1.3","Lecci, L. S. & Righi-Cavallaro, K. O. (2017) Checklist de Plecoptera (Insecta) do Estado do Mato Grosso do Sul, Brasil. Iheringia, 107, 1 - 2. https: // doi. org / 10.1590 / 1678 - 4766 e 2017118"]}

Anacroneuria dourada Jewett, 1960 (Figs. 5A–D) Anacroneuria dourada Jewett, 1960b: 2; Illies, 1966: 316; Froehlich, 2010: 154. Material examined (picture). BR, GO. Paratype: Formoso, 9.vi.1956, F.S. T., 1 female (NHM). Remarks. The species was described by Jewett (1960b) based on specimens from the region of Formoso and São João da Aliança, in the north of Goiás state. According to the author, the type series would be deposited in the National Museum of Rio de Janeiro, RJ, BR (holotype) and NHM (Natural History Museum, Los Angeles County), CA, USA (paratypes). However, the holotype is misplaced. Fortunately, a paratype was located in the NHM. The specimen is pinned female. Despite being well preserved and having a color pattern corresponding to the original description (Jewett 1960b), in the picture (Fig. 5) the subgenital plate is a little collapsed, not allowing a deeper analysis. The morphology of this paratype needs to be carefully studied before checking the possibility of associating it with the specimens collected in the type locality in north Goiás state. Additionally, the fact that the paratype is female can hinder this process., Published as part of Miguel, Marina, Almeida, Lucas Henrique De, Novaes, Marcos Carneiro & Bispo, Pitágoras Da Conceição, 2022, Anacroneuria (Plecoptera: Perlidae) from Central Brazil: new species, new geographic records, and taxonomic notes, pp. 487-506 in Zootaxa 5162 (5) on page 493, DOI: 10.11646/zootaxa.5162.5.2, http://zenodo.org/record/6816755, {"references":["Jewett, S. G. (1960 b) Two new species of Anacroneuria (Plecoptera) from Goias, Brazil. Contributions in Science, 36, 1 - 4. https: // doi. org / 10.5962 / p. 241035","Illies, J. (1966) Katalog der rezenten Plecoptera. Das Tierreich, Berlin, 82, 1 - 632 + xxx.","Froehlich, C. G. (2010) Catalogue of Neotropical Plecoptera. Illiesia, 6 (12), 118 - 205."]}

Anacroneuria brandaoi Bispo & Froehlich, 2004 (Figs. 3A–H) Anacroneuria brandaoi Bispo & Froehlich, 2004: 193; Froehlich, 2010: 149. Material examined. BR, GO. Pirenópolis, Rio das Almas, 15°50'59.2"S 48°53'23.8"W, 09.ix.1993, LGO, 1 male (CLBA); Córrego Vagafogo, 15°49'27.8"S 48°59'43.8"W, 10.ix.1993, LGO, 1 male (CLBA); Córrego Vagafogo, 15.ix.1993, LGO, 1 male (CLBA); Rio das Almas, 10.x.1993, LGO, 3 males (CLBA); Rio das Almas, 14.xi.1993, LGO, 6 males (CLBA); Rio das Almas, 12.xii.1993, LGO, 3 males (CLBA); Rio das Almas, 14.i.1994, PCB & LGO, 1 male (CLBA); Córrego Vagafogo, 15.i.1994, PCB & LGO, 1 male (CLBA); Rio das Almas, 03.ii.1994, LGO, 1 male (CLBA); Córrego Inferno, 12.iii.1994, LGO, 1 male (CLBA); Rio das Almas, 13.iii. 1994, LGO, 2 males (CLBA); Córrego inferno, 09.iv.1994. PCB & LGO, 1 male (CLBA); Rio das Almas, 10.iv.1994, LGO, 1 male (CLBA); Rio das Almas, 14.v.1994, LGO, 2 males (CLBA); 21.x.1995, JBS, 1 male (CLBA). Chapada dos Veadeiros, Alto Paraíso de Goiás, Ribeirão Couro, Cachoeira São Bento, 14°09'38.3"S 47°35'38.8"W, 13.xii.2006, PCB, 1 male (CLBA). Morphometric data. Male (n=28), anterior wing length: 11.0–13.0 mm. Remarks. Anacroneuria brandaoi was first described at the Serra da Mesa, in the north of Goiás state (Bispo & Froehlich 2004). In this work, we recorded the species in the Chapada dos Veadeiros and Serra dos Pireneus, Goiás state. The penial armature of A. brandaoi differs from that of the holotype of A. debilis (Zwick 1972). However, recent studies of different populations of A. debilis (Baldin et al. 2013; Bispo et al. 2014; Novaes & Bispo 2014a) revealed high variability in the penial armature, which could overlap with that of A. brandoi. Herein, we observed that the penial armature of A. brandaoi has a more rounded keel and that the apex tapers less abruptly (Fig. 3) than that of A. debilis from the same location.

Anacroneuria corae Bispo & Novaes, 2014 (Figs. 2D–F) Anacroneuria corae Bispo & Novaes in Bispo et al., 2014: 592; Rippel et al., 2019: 359. Material examined. BR, GO. Pirenópolis, Córrego Inferno, 09.x.1993, LGO, 1 male (CLBA); Córrego Inferno, 11.xii.1993, PCB & LGO, 1 male (CLBA). Morphometric data. Male (n=2), anterior wing length: 10 mm. Remarks. Anacroneuria corae was collected at the Serra dos Pireneus, Goiás state. This species was originally described in the Cidade de Goiás, Goiás state (Bispo et al. 2014). Recently, the geographic distribution of the species was expanded to Tocantins state (Rippel et al. 2019). The specimens collected agree with the holotype. However, one of the specimens studied did not show wing with a distal less pigmented circular area (Figs. 2E–F), characteristic of the species (Bispo et al. 2014)., Published as part of Miguel, Marina, Almeida, Lucas Henrique De, Novaes, Marcos Carneiro & Bispo, Pitágoras Da Conceição, 2022, Anacroneuria (Plecoptera: Perlidae) from Central Brazil: new species, new geographic records, and taxonomic notes, pp. 487-506 in Zootaxa 5162 (5) on page 493, DOI: 10.11646/zootaxa.5162.5.2, http://zenodo.org/record/6816755, {"references":["Bispo, P. C., Costa, L. S. M. & Novaes, M. C. (2014) Two new species and a new record of Anacroneuria (Plecoptera: Perlidae) from Central Brazil. Zootaxa, 3779 (5), 591 - 596. https: // doi. org / 10.11646 / zootaxa. 3779.5.9","Rippel, M. L. S., Novaes, M. C. & Krolow, T. K. (2019) First records of genus Anacroneuria (Plecoptera: Perlidae) from Tocantins State, Brazil and description of a new species. Zootaxa, 4560 (2), 355 - 364. https: // doi. org / 10.11646 / zootaxa. 4560.2.8"]}

Anacroneuria singela Duarte & Lecci, 2016 (Figs.10 A-G) Anacroneuria singela Duarte & Lecci, 2016: 297; Almeida & Duarte, 2017: 482. Material examined. BR, GO. Pirenópolis, 29.x.1994, MVLV, 1 male (CLBA). Morphometric data. Male (n=1), anterior wing length: 12.0 mm. Remarks. The specimen studied agrees with the description. It is the first time that this species is recorded in Goiás state. As already highlighted by Duarte & Lecci (2016), A. singela is similar to A. debilis; however, A. singela has a more robust penial armature, especially in the keel and terminal tube., Published as part of Miguel, Marina, Almeida, Lucas Henrique De, Novaes, Marcos Carneiro & Bispo, Pitágoras Da Conceição, 2022, Anacroneuria (Plecoptera: Perlidae) from Central Brazil: new species, new geographic records, and taxonomic notes, pp. 487-506 in Zootaxa 5162 (5) on page 499, DOI: 10.11646/zootaxa.5162.5.2, http://zenodo.org/record/6816755, {"references":["Duarte, T. & Lecci, L. S. (2016) New species and records of Anacroneuria (Plecoptera: Perlidae) from the northeastern semi-arid region of Brazil. Zootaxa, 4079 (2), 291 - 300. https: // doi. org / 10.11646 / zootaxa. 4079.2.10","Almeida, L. H. & Duarte, T. (2017) A new species and records of Anacroneuria (Plecoptera: Perlidae) from the Ecological Station, Wenceslau Guimaraes, state of Bahia, Brazil. Zootaxa, 4247 (4), 480 - 486. https: // doi. org / 10.11646 / zootaxa. 4247.4.9"]}

Anacroneuria amargosa Righi-Cavallaro & Froehlich, 2013 (Figs. 2A–C) Anacroneuria amargosa Righi-Cavallaro & Froehlich in Righi-Cavallaro et al., 2013: 1; Duarte & Lecci, 2016: 293; Gonçalves et al., 2017: 146. Material examined. BR, GO. Pirenópolis, Córrego do Inferno, 15°47'34.1"S 48°50'40.0"W, 13.xi.1993, LGO, 1 male (CLBA). Morphometric data. Male (n=1), anterior wing length: 9.1 mm. Remarks. Anacroneuria amargosa was collected at Serra dos Pireneus and is a new record for Goiás state. This species was originally described in Mid-South Mesoregion of the Bahia state, and the present study expands the distribution to Central Brazil. The collected specimen is similar to the holotype described by Righi-Cavallaro & Froehlich in Righi-Cavallaro et al. (2013) and agrees in color, size, and penial armature shape. As commented by Righi-Cavallaro et al. (2013), the penial armature resembles Anacroneuria boraceiensis Froehlich, 2004.

Anacroneuria minuta Klapálek, 1922 (Figs. 7D–F) Anacroneuria minuta Klapálek, 1922: 89; Illies, 1966: 319; Kimmins, 1970: 345; Ribeiro-Ferreira & Froehlich, 2001: 188; Froehlich, 2002: 80; Ribeiro & Rafael, 2009: 2; Froehlich, 2010: 162; Ribeiro & Gorayeb, 2014: 23; Ribeiro & Gorayeb, 2016: 434; Rippel et al., 2019: 360; Menezes et al., 2020: 468; Carvalho et al., 2020: 439. Material examined. BR, GO. Pirenópolis, Córrego Inferno, 09.x.1993, LGO, 2 males (CLBA); Rio das Almas, 09.ix.1993, LGO, 4 males (CLBA); Córrego Vagafogo, 15.ix.1993, LGO, 3 males (CLBA); Rio das Almas, 14.xi.1993, LGO, 10 males (CLBA); Rio das Almas, 13.iii.1994, LGO, 1 male (CLBA); Rio das Almas, 13.iii.1994, LGO, 1 male (CLBA); 29.x.1994, CL, 1 male (CLBA). Chapada dos Veadeiros, Alto Paraíso de Goiás, Ribeirão Saltador, 14°06'07.2"S 47°29'59.1"W, 12.xii.2006, PCB, 2 males (CLBA); Ribeirão Couro, Cachoeira São Bento, 13.xii.2006, PCB, 1 male (CLBA). Morphometric data. Male (n=25), anterior wing length: 9.0– 11.5 mm. Remarks. Anacroneuria minuta was collected at Chapada dos Veadeiros and Serra dos Pireneus, and is a new record for the Goiás state. Although previously recorded in Amazonas, Pará, Roraima, and Tocantins states, the present study now expands the distribution of this species to Goiás state. The studied specimens are typical and strongly resemble those illustrated by Froehlich (2002). The nymph was described by Ribeiro & Gorayeb (2014)., Published as part of Miguel, Marina, Almeida, Lucas Henrique De, Novaes, Marcos Carneiro & Bispo, Pitágoras Da Conceição, 2022, Anacroneuria (Plecoptera: Perlidae) from Central Brazil: new species, new geographic records, and taxonomic notes, pp. 487-506 in Zootaxa 5162 (5) on page 496, DOI: 10.11646/zootaxa.5162.5.2, http://zenodo.org/record/6816755, {"references":["Klapalek, F. (1922) Plecopteres nouveaux. Troisieme partie. Annales de la Societe Entomologique de Belgique, 61, 320 - 327.","Illies, J. (1966) Katalog der rezenten Plecoptera. Das Tierreich, Berlin, 82, 1 - 632 + xxx.","Kimmins, D. G. (1970) A list of the type-specimens of Plecoptera and Megaloptera in the British Museum (Natural History). Bulletin of the British Museum of Natural History, 24, 335 - 361. https: // doi. org / 10.5962 / bhl. part. 1523","Ribeiro-Ferreira, A. C. & Froehlich, C. G. (2001) Anacroneuria Klapalek, 1909 from Amazonas State, North Brazil (Plecoptera, Perlidae, Anacroneuriinae). Aquatic Insects, 23 (3), 187 - 192. https: // doi. org / 10.1076 / aqin. 23.3.187.4890","Froehlich, C. G. (2002) Anacroneuria mainly from southern Brazil and northeastern Argentina (Plecoptera: Perlidae). Proceedings of the Biological Society of Washington, 115, 75 - 107.","Ribeiro, J. M. F. & Rafael, J. A. (2009) Redescription of the species of Anacroneuria Klapalek known from the Reserva Florestal Adolpho Ducke, Amazonas, Brazil, with a neotype designation, and a key to adult males, Amazonas, Brazil (Plecoptera, Perlidae). Zootaxa, 2004 (1), 1 - 15. https: // doi. org / 10.11646 / zootaxa. 2004.1.1","Froehlich, C. G. (2010) Catalogue of Neotropical Plecoptera. Illiesia, 6 (12), 118 - 205.","Ribeiro, J. M. F. & Gorayeb, I. S. (2014) Description of immatures and association with adults of three species of Anacroneuria Klapalek (Plecoptera: Perlidae) of the Brazilian Amazon. Zootaxa, 3881 (1), 17 - 32. https: // doi. org / 10.11646 / zootaxa. 3881.1.2","Ribeiro, J. M. F. & Gorayeb, I. S. (2016) Description of immatures associated with adults of two species of Macrogynoplax Enderlein and one species of Enderleina Jewett (Plecoptera: Perlidae) of the Brazilian Amazon. Zootaxa, 3957 (1), 69 - 76. https: // doi. org / 10.11646 / zootaxa. 3948.1.7","Rippel, M. L. S., Novaes, M. C. & Krolow, T. K. (2019) First records of genus Anacroneuria (Plecoptera: Perlidae) from Tocantins State, Brazil and description of a new species. Zootaxa, 4560 (2), 355 - 364. https: // doi. org / 10.11646 / zootaxa. 4560.2.8","Menezes, E. T., Boldrini, R. & Novaes, M. C. (2020) New species of the genus Macrogynoplax and new records of Anacroneuria (Plecoptera: Perlidae) from Roraima State, Brazil. Zootaxa, 4722 (5), 463 - 471. https: // doi. org / 10.11646 / zootaxa. 4722.5.4","Carvalho, A. S., Almeida, L. H., Azevedo, C. A. S. & Lima, L. R. (2020) The genus Anacroneuria Klapalek, 1909 (Plecoptera: Perlidae) from Maranhao State, northeastern Brazil. Zootaxa, 4860 (3), 435 - 44. https: // doi. org / 10.11646 / zootaxa. 4860.3.8"]}

Anacroneuria ofaye Froehlich, 2007 (Figs. 8A–G) Anacroneuria ofaye Froehlich, 2007: 16; Froehlich, 2010: 164; Righi-Cavallaro & Lecci, 2010:36-37; Novaes et al., 2012: 71; Novaes & Bispo, 2014b: 278 Lecci & Righi-Cavallaro, 2017: 2. Material examined. BR, GO. Pirenópolis, Córrego Inferno; 08.ix.1993, LGO, 2 males (CLBA); Córrego inferno, 09. x.1993, LGO, 1 male (CLBA); Córrego Inferno, 13.xi.1993, LGO, 1 male (CLBA). Morphometric data. Male (n=4), anterior wing length: 10 mm. Remarks. The occurrence of Anacroneuria ofaye is a new record for the Goiás state. The penial armature of the studied specimens is similar to that described by Froehlich (2007), both in shape and size. However, like in Anacroneuria brandaoi, the variations in penial armature found in A. ofaye may slightly overlap those observed in A. debilis. The nymph was described by Novaes et al. (2012)., Published as part of Miguel, Marina, Almeida, Lucas Henrique De, Novaes, Marcos Carneiro & Bispo, Pitágoras Da Conceição, 2022, Anacroneuria (Plecoptera: Perlidae) from Central Brazil: new species, new geographic records, and taxonomic notes, pp. 487-506 in Zootaxa 5162 (5) on page 498, DOI: 10.11646/zootaxa.5162.5.2, http://zenodo.org/record/6816755, {"references":["Froehlich, C. G. (2007) Three new species of Anacroneuria (Plecoptera: Perlidae) from State of Mato Grosso do Sul, Brazil. Zootaxa, 1461 (1), 15 - 24. https: // doi. org / 10.11646 / zootaxa. 1461.1.2","Froehlich, C. G. (2010) Catalogue of Neotropical Plecoptera. Illiesia, 6 (12), 118 - 205.","Righi-Cavallaro, K. O. & Lecci, L. S. (2010) Three new species of Anacroneuria (Plecoptera: Perlidae) from Centre-West and Southeast Brazil. Zootaxa, 2683 (1), 35 - 44. https: // doi. org / 10.11646 / zootaxa. 2683.1.3","Novaes, M. C., Bispo, P. C. & Higuti, J. (2012) A description of the nymph of Anacroneuria ofaye Froehlich (Plecoptera: Perlidae) and an apparatus for rearing Neotropical stonefly species. Zootaxa, 3547 (1), 71 - 77. https: // doi. org / 10.11646 / zootaxa. 3547.1.7","Novaes, M. C. & Bispo, P. C. (2014 b) Perlidae (Plecoptera) from Southeastern Santa Catarina State, Southern Brazil. Zootaxa, 3779 (2), 277 - 287. https: // doi. org / 10.11646 / zootaxa. 3779.2.8","Lecci, L. S. & Righi-Cavallaro, K. O. (2017) Checklist de Plecoptera (Insecta) do Estado do Mato Grosso do Sul, Brasil. Iheringia, 107, 1 - 2. https: // doi. org / 10.1590 / 1678 - 4766 e 2017118"]}

Anacroneuria debilis (Pictet, 1841) (Figs. 4A–P) Perla (Perla) debilis Pictet, 1841: 255. Anacroneuria debilis Zwick, 1972: 1155; Zwick, 1973: 486; Froehlich, 2002: 76; Avelino Capistrano et al., 2011: 59–63; Baldin et al., 2013: 392; Bispo et al., 2014: 592; Novaes & Bispo, 2014a: 434; Novaes & Bispo, 2014b: 459; Novaes et al., 2016: 95; Duarte & Lecci, 2016: 293; Almeida & Duarte, 2017: 481; Gonçalves et al., 2017: 146; Rippel et al., 2019: 359. Material examined. BR, GO. Parque Nacional das Emas, Chapadão do Céu, Luz, sede, prox. ao Rio Formoso, 18°15'40.7"S 52°53'18.5"W, 28.iii - 06.iv.1984, SAV, DA, 2 males (CLBA). Pirenópolis, Rio das Almas, 14.xi.1993, LGO, 1 male (CLBA); Córrego Inferno, 02.ii.1994, PCB & LGO, 1 male (CLBA); Córrego inferno, 09.iv.1994, PCB & LGO, 3 males (CLBA); 29.x.1994, MVLV, 1 male (CLBA). Chapada dos Veadeiros, Alto Paraíso de Goiás, Ribeirão Raizama, 14°10'51.7"S 47°49'18.5"W, 10.xii.2006, PCB, 1 male (CLBA); Ribeirão Couro, Cachoeira São Bento, 13.xii.2006, PCB, 6 males (CLBA); Loquinhas, 14°08'33.1"S 47°29'23.3"W, 15.xii.2006, PCB, 1 male (CLBA); Vale da Lua, 14°11'11.5"S 47°47'21.1"W, 17.xii.2006, PCB, 2 males (CLBA). Morphometric data. Male (n=18), anterior wing length: 8 mm – 13 mm. Remarks. Anacroneuria debilis was originally described by Pictet (1841) and the penial armature was illustrated by Zwick (1972). In Central Brazil, the species was recorded in the region of the Cidade de Goiás (Bispo et al. 2014), and in the present study we expanded the records for the Serra dos Pireneus, Chapada dos Veadeiros, and Emas National Park, Goiás state. Recent studies revealed that this species has a large distribution and high morphological variation (Bispo et al. 2014; Novaes & Bispo 2014c; Almeida & Duarte 2017). We also found a large variation in the penial armatures of the males of A. debilis, especially in the keel and apex (Fig. 4). As indicated by previous studies (Novaes & Bispo 2014c; Almeida & Duarte 2017) and our results, A. debilis may be composed of a group of cryptic species. Therefore, a more integrative study based on both morphological and molecular data is required for a better definition of this species., Published as part of Miguel, Marina, Almeida, Lucas Henrique De, Novaes, Marcos Carneiro & Bispo, Pitágoras Da Conceição, 2022, Anacroneuria (Plecoptera: Perlidae) from Central Brazil: new species, new geographic records, and taxonomic notes, pp. 487-506 in Zootaxa 5162 (5) on page 493, DOI: 10.11646/zootaxa.5162.5.2, http://zenodo.org/record/6816755, {"references":["Pictet, F. J. (1841) Histoire Naturelle Generale et Particuliere des Insectes Nevropteres. Famille des Perlides. J. Kessmann, Geneve, 423 pp. https: // doi. org / 10.5962 / bhl. title. 124172","Zwick, P. (1972) Die Plecopteren Pictets und Burmeisters, mit Angaben uber weitere Arten (Insecta). Revue Suisse de Zoologie, 78, 1123 - 1194. https: // doi. org / 10.5962 / bhl. part. 97087","Zwick, P. (1973) Insecta Plecoptera. Phylogenetisches System und Katalog. Das Tierreich, Berlin, 94, 1 - 465 + xxxii.","Froehlich, C. G. (2002) Anacroneuria mainly from southern Brazil and northeastern Argentina (Plecoptera: Perlidae). Proceedings of the Biological Society of Washington, 115, 75 - 107.","Baldin, C., Bispo P. C. & Novaes, M. C. (2013) New species and records of Anacroneuria (Plecoptera: Perlidae) from Rio de Janeiro State, Brazil. Zootaxa, 3694 (4), 391 - 397. https: // doi. org / 10.11646 / zootaxa. 3694.4.7","Bispo, P. C., Costa, L. S. M. & Novaes, M. C. (2014) Two new species and a new record of Anacroneuria (Plecoptera: Perlidae) from Central Brazil. Zootaxa, 3779 (5), 591 - 596. https: // doi. org / 10.11646 / zootaxa. 3779.5.9","Novaes, M. C. & Bispo, P. C. (2014 a) A new species and notes on Perlidae (Plecoptera) from Parana and Santa Catarina States, southern Brazil. Zootaxa, 3765 (5), 458 - 468. https: // doi. org / 10.11646 / zootaxa. 3765.5.4","Novaes, M. C. & Bispo, P. C. (2014 b) Perlidae (Plecoptera) from Southeastern Santa Catarina State, Southern Brazil. Zootaxa, 3779 (2), 277 - 287. https: // doi. org / 10.11646 / zootaxa. 3779.2.8","Novaes, M. C., Bispo, P. C. & Goncalves, M. C. (2016) A new species of Anacroneuria Klapalek 1909 (Plecoptera: Perlidae) from Espirito Santo State, southeastern Brazil and comments on additional species. Zootaxa, 4208 (1), 94 - 98. https: // doi. org / 10.11646 / zootaxa. 4208.1.7","Duarte, T. & Lecci, L. S. (2016) New species and records of Anacroneuria (Plecoptera: Perlidae) from the northeastern semi-arid region of Brazil. Zootaxa, 4079 (2), 291 - 300. https: // doi. org / 10.11646 / zootaxa. 4079.2.10","Almeida, L. H. & Duarte, T. (2017) A new species and records of Anacroneuria (Plecoptera: Perlidae) from the Ecological Station, Wenceslau Guimaraes, state of Bahia, Brazil. Zootaxa, 4247 (4), 480 - 486. https: // doi. org / 10.11646 / zootaxa. 4247.4.9","Goncalves, M. C., Novaes, M. C. & Salles, F. F. (2017) New species and records of Perlidae (Plecoptera) from Espirito Santo State, Brazil. Zootaxa, 4273 (1), 141 - 150. https: // doi. org / 10.11646 / zootaxa. 4273.1.11","Rippel, M. L. S., Novaes, M. C. & Krolow, T. K. (2019) First records of genus Anacroneuria (Plecoptera: Perlidae) from Tocantins State, Brazil and description of a new species. Zootaxa, 4560 (2), 355 - 364. https: // doi. org / 10.11646 / zootaxa. 4560.2.8","Novaes, M. C. & Bispo, P. C. (2014 c) Plecoptera from Minas Gerais State, southeastern Brazil. Zootaxa, 3856 (3), 433 - 442. https: // doi. org / 10.11646 / zootaxa. 3856.3.8"]}

Anacroneuria mineira Novaes & Bispo, 2014 (Figs. 7A–C) Anacroneuria mineira Novaes & Bispo, 2014c: 433. Material examined. BR, GO. Pirenópolis, 28.x.1994, ABV, 1 male; 28.x.1994, ABV, 3 males (CLBA); Pirenópolis, 28.x.1994, R. César, 3 males (CLBA); 28.x.1994, HLRP, 3 males (CLBA); 28.x.1994, MVLV, 5 males (CLBA); 29.x.1994, CL, 2 males (CLBA); 21.x.1995, LRPL, 1 male (CLBA). Morphometric data. Male (n=18), anterior wing length: 11.0–15.0 mm. Remarks. Anacroneuria mineira was collected at Serra dos Pireneus and is a new record for the Goiás state. This species was originally described at Serra do Cipó, Minas Gerais state (Novaes & Bispo 2014c), and the present study now expands the distribution to Central Brazil. The specimens studied herein vary in size (anterior wing 11-15 mm) and are smaller than the holotype, which has an anterior wing around 16 mm (Novaes & Bispo 2014c)., Published as part of Miguel, Marina, Almeida, Lucas Henrique De, Novaes, Marcos Carneiro & Bispo, Pitágoras Da Conceição, 2022, Anacroneuria (Plecoptera: Perlidae) from Central Brazil: new species, new geographic records, and taxonomic notes, pp. 487-506 in Zootaxa 5162 (5) on page 495, DOI: 10.11646/zootaxa.5162.5.2, http://zenodo.org/record/6816755, {"references":["Bispo, P. C., Costa, L. S. M. & Novaes, M. C. (2014) Two new species and a new record of Anacroneuria (Plecoptera: Perlidae) from Central Brazil. Zootaxa, 3779 (5), 591 - 596. https: // doi. org / 10.11646 / zootaxa. 3779.5.9","Novaes, M. C. & Bispo, P. C. (2014 c) Plecoptera from Minas Gerais State, southeastern Brazil. Zootaxa, 3856 (3), 433 - 442. https: // doi. org / 10.11646 / zootaxa. 3856.3.8"]}

Anacroneuria galba Jewett, 1960 (Figs. 6A–D) Anacroneuria galba Jewett, 1960b: 3; Illies, 1966: 317; Froehlich, 2010: 156. Material examined (picture). BR, GO. Paratype: Formoso, 26.v.1956, F.S. T., 1 male (NHM). Remarks. The species was described by Jewett (1960b) based on specimens from the region of Formoso, in the north of Goiás state, BR. Similarly to A. dourada, according to the original description the type series is deposited in the National Museum of Rio de Janeiro, RJ, BR (holotype) and NHM, CA, USA (paratypes). However, the holotype is also currently lost. Fortunately, a dry preserved paratype is located in the NHM. The male paratype is well preserved. Its genitalia has not been dissected and there are no illustrations of the penial armature of the species. Therefore, the morphology of this paratype, including the morphology of the penial armature, needs to be carefully studied before trying to associate it with specimens collected in the type locality in north of Goiás state. The fact that the paratype is male can facilitate this process. The other specimens of A. galba cited by Jewett (1960b) for other localities (Espírito Santo, Minas Gerais, and São Paulo states) probably belong to other species (Froehlich 2010)., Published as part of Miguel, Marina, Almeida, Lucas Henrique De, Novaes, Marcos Carneiro & Bispo, Pitágoras Da Conceição, 2022, Anacroneuria (Plecoptera: Perlidae) from Central Brazil: new species, new geographic records, and taxonomic notes, pp. 487-506 in Zootaxa 5162 (5) on page 494, DOI: 10.11646/zootaxa.5162.5.2, http://zenodo.org/record/6816755, {"references":["Jewett, S. G. (1960 b) Two new species of Anacroneuria (Plecoptera) from Goias, Brazil. Contributions in Science, 36, 1 - 4. https: // doi. org / 10.5962 / p. 241035","Illies, J. (1966) Katalog der rezenten Plecoptera. Das Tierreich, Berlin, 82, 1 - 632 + xxx.","Froehlich, C. G. (2010) Catalogue of Neotropical Plecoptera. Illiesia, 6 (12), 118 - 205."]}

Anacroneuria minuta Klap��lek, 1922 Anacroneuria minuta, Klap��lek 1922: 89; Illies 1966: 319; Kimmins 1970: 345; Ribeiro-Ferreira & Froehlich 2001: 188; Froehlich 2002: 80; Ribeiro & Rafael 2009: 2; Ribeiro & Gorayeb 2016: 434; Rippel et al. 2019: 360. Material examined. BRA, RR, Amajari, Serra do Tepequ��m, Igarap�� Barata, 03��47���07.07���N / 61��45���02.06���W. 16.ii.2016, light trap, 5 males and 2 females, R. Boldrini & L. R. C. Lima (UFRR). BRA, RR, Cant��, Serra Grande, 02��32���29.02���N / 60��47���53.06���W, 07.x.2015, light trap, 1 male, P. C. S. Barroso & R. Boldrini (UFRR); 19.x.2016, light trap, 3 males, P. C. S. Barroso & R. Boldrini (UFRR). A. minuta variant 1 - BRA, RR, Pacaraima, Rio Surumu, 04��15���17.00������N / 61��02���05.00������W, 11.x.2015, light trap, 2 males and 4 females, P. C. S. Barroso & R. Boldrini (UFRR). BRA, RR, Amajari, Rio Acari, S��tio Baixa Da ��gua, Regi��o Do Ereu, 04��04���28.10���N / 61��27���09.50���W, 15���17.xi.2018, Pennsylvania trap, 1 male, B. C. Souza (UFRR); BRA, RR, Alto Alegre, FLONA Roraima, 02�� 56���18.05���N / 61��37���27.28���W, 19.xii.2017, light trap, 2 females, R. Boldrini (UFRR); 20.xii.2017, light trap, 1 female, R. Boldrini (UFRR); 21.xii.2017, light trap, 1 female, R. Boldrini (UFRR); 01���15.ii.2018, Malaise trap, 1 male and 1 female, R. Boldrini (UFRR), 15���30.iii.2018, Malaise trap, 4 males and 1 female, R. Boldrini (UFRR); 02���17.v.2018, Malaise trap, 3 males and 1 female, R. Boldrini (UFRR); 01���13.vii.2018, Malaise trap, 1 female, R. Boldrini (UFRR). A. minuta variant 2 ���BRA, RR, Boa Vista, UFRR, Campus Experimental Cauam�� (CCA), 02��51���47.76���N / 60��42���53.89���W, 10.vi.2017, light trap, 32 males and 4 females, R. Boldrini, E. T. Menezes & I. B. Oliveira (UFRR). Measurements data. A. minuta. Female forewing length: 10.5���14.0 mm, n= 02; male forewing length: 7.0���9.0 mm, n= 9. A. minuta variant 1. Female forewing length: 9.1���10.0 mm, n= 12; male forewing length: 7.0���8.0 mm, n= 11. A. minuta variant 2. Female forewing length: 10.0���12.0 mm, n= 4; male forewing length: 8.1���9.0 mm, n= 32. Remarks. This species occurs in the Roraima State in addition to the states of Amazonas, Par��, and Tocantins. (Rippel et al. 2019). During our sampling, we found two additional morphotypes, with minor variation in the pigmentation of the wing veins Sc and R 1 in A. minuta variant 1 where they vary from hyaline to darker, as well a wider lateral stripe on the pronotum of male. Another variation, detected was the subgenital plate of female with only two lobes in A. minuta variant 2, whereas four lobes in A. minuta variant 1 and typical A. minuta. The apex of the penis is bifid in A. minuta variant 1 and 2. A. minuta variant 1��� Male forewing length: 7���8 mm, length: 7 mm, penis length 0.07 mm and 0.03 mm width. Penis conical same as the previous species. Regularly curved hooks, with pointed apex, slightly shorter than the previous species. In lateral view, the dorsal keel is apparently shortened and spiculated. In ventral view, there are two of small membranous vesicles. The penis apex is short and tapered in lateral view with apex bifurcate in dorsal view. Wings with darker veins, except costa. Subgenital plate is wide with four undulating lobes, and the medial notch is deeper than the submedial notches. A. minuta variant 2��� length 9 mm, penis length 0.07 mm and 0.03 mm width. Penis: generally conical according to previous species. Regularly curved hooks, with pointed apex, slightly shorter than A. minuta, but similar to A. minuta variant 1. In lateral view, there is a slight depression in front of the dorsal keel, discernably spiculated and bifid. In ventral view, there are two small membranous vesicles. The apex of the penis is short and acute in lateral view. The bifuricate apex of the penis in dorsal view similar to A. minuta variant 1. Subgenital plate is wide with two lobes, with a deep medial notch., Published as part of Menezes, Emely Trajano De, Boldrini, Rafael & Novaes, Marcos Carneiro, 2020, New species of the genus Macrogynoplax and new records of Anacroneuria (Plecoptera: Perlidae) from Roraima State, Brazil, pp. 463-471 in Zootaxa 4722 (5) on pages 468-469, DOI: 10.11646/zootaxa.4722.5.4, http://zenodo.org/record/3609700, {"references":["Klapalek, F. (1922) Plecopteres nouveaux. Quatrieme partie. Annales de la Societe Entomologique de Belgique, 62, 89 - 95.","Illies, J. (1966) Katalog der rezenten Plecoptera. Das Tierreich 82. Walter de Gruyter & Co, Berlin, xxx + 632 pp.","Kimmins, D. E. (1970) A list of the type-specimens of Plecoptera and Megaloptera in the British Museum (Natural History). Bulletin of the British Museum (Natural History) Entomology, 24 (8), 335 - 361. https: // doi. org / 10.5962 / bhl. part. 1523","Ribeiro-Ferreira, A. C. & Froehlich, C. G. (2001) Anacroneuria Klapalek, 1909 from Amazonas State, North Brazil (Plecoptera, Perlidae, Acroneuriinae). Aquatic Insects, 23 (3), 187 - 192. https: // doi. org / 10.1076 / aqin. 23.3.187.4890","Froehlich, C. G. (2002) Anacroneuria mainly from southern Brazil and northeastern Argentina (Plecoptera: Perlidae). Proceedings of the Biological Society of Washington, 115 (1), 75 - 107.","Ribeiro, J. M. F. & Rafael, J. A. (2009) Redescription of the species of Anacroneuria Klapalek known from the Reserva Florestal Adolpho Ducke, Amazonas, Brazil with a neotype designation, and a key to adults males, Amazonas, Brazil. (Plecoptera, Perlidae). Zootaxa, 2004 (1), 1 - 15. https: // doi. org / 10.11646 / zootaxa. 2004.1.1","Ribeiro, J. M. F. & Gorayeb, I. S. (2016) Key to Plecoptera nymphs from the Brazilian Amazon (Insecta). Zootaxa, 4208 (5), 433 - 445. https: // doi. org / 10.11646 / zootaxa. 4208.5.2"]}

Macrogynoplax quadrispina n. sp. (Figs. 2 A–E) Material examined. Holotype male: BRAZIL (BRA), Roraima State (RR), Municipality of Amajari, Serra do Tepequém, Igarapé Cocal, 03°45’12.00”N / 61°42’42.00”W, 03–13.xi.2017, Malaise trap, R. Boldrini (INPA). Paratypes: same locality, 03–13.xi.2017, Malaise trap, 1 male; 13–23.xi.2017, Malaise trap, 1 female, R. Boldrini (UFRR). Description. General color pale yellow in ethanol. Head with M-line not distinct; gena light brown; maxillary and labial palps light brown; antennae dark yellow, with scape and pedicel light yellow; compound eyes dark and with inner margin not pigmented; ocelli with narrow dark ring (Fig. 2A). Coxa and trochanter pale yellow, first and third pair of legs with entirely brown tibia and femur. Wings yellow with veins darker except veins Sc. Cerci dark yellow. Male. Forewing length 9.8–10.2 mm (n= 2). Ninth sternite with a pair of basolateral protuberances; hammer U-shape (Fig. 2E). Penial amature with ventromedial membranous bowl-shaped section, with of four hooks, one pair lateroapically smaller and tapered, other pair posteriorly laterally longer and thicker with spines at the apex; the apex of the hooks are darkly sclerotized (Figs. 2 B-D). Female. Forewing length 12.6–13.0 mm (n= 1). Head larger than the male, coloration similar to male. Subgenital plate notched, not reaching beyond the posterior margin of the sternum 9. The lateral bars are fused to the sternum 9. Nymph. Unknown. Remarks. Specimens have faded in ethanol. The presence of four penial hook-like structures is unusual and unique among the known species of Macrogynoplax, easily distinguishing the male of the new species from all other described species. Etymology. From word combination of the Latin quadr and spin, for the four hooks of the penial amature.

Anacroneuria pictipes Klap��lek, 1923 Anacroneuria pictipes Klap��lek 1923: 21; Illies 1966: 321; Stark 1999: 83���85; Ribeiro & Gorayeb 2016: 434. Material examined. BRA, RR, Pacaraima, Igarap�� Sam��, 04��28���30.08������N / 61��09���44.04������W, Len��ol and Pennsylvania, 10.x.2015, Pennsylvania trap, 2 males, R. Boldrini (UFRR); BRA, RR, Amajari, Serra do Tepequ��m, 03��45���12.00���N / 61��42���42.00���W, Malaise trap, 13���23.x.2017, 6 males, R. Boldrini (UFRR). Measurements data. Male forewing: 10.0 mm, n= 6. Remarks. This s pecies has been recorded for Guyana and from the Brazilian state of Amap�� (Ribeiro & Gorayeb, 2016). Here, we present the first record for Roraima State., Published as part of Menezes, Emely Trajano De, Boldrini, Rafael & Novaes, Marcos Carneiro, 2020, New species of the genus Macrogynoplax and new records of Anacroneuria (Plecoptera: Perlidae) from Roraima State, Brazil, pp. 463-471 in Zootaxa 4722 (5) on page 469, DOI: 10.11646/zootaxa.4722.5.4, http://zenodo.org/record/3609700, {"references":["Klapalek, F. (1923) Plecopteres nouveaux. Cinquieme partie. Annales de la Societe Entomologique de Belgique, 63, 21 - 29.","Illies, J. (1966) Katalog der rezenten Plecoptera. Das Tierreich 82. Walter de Gruyter & Co, Berlin, xxx + 632 pp.","Stark, B. P. (1999) Anacroneuria from northeastern South America (Insecta: Plecoptera: Perlidae). Proceedings of the Biological Society of Washington, 112 (1), 70 - 93.","Ribeiro, J. M. F. & Gorayeb, I. S. (2016) Key to Plecoptera nymphs from the Brazilian Amazon (Insecta). Zootaxa, 4208 (5), 433 - 445. https: // doi. org / 10.11646 / zootaxa. 4208.5.2"]}

Anacroneuria cruza Stark, 1995 Stark 1995: 232; Stark 1999: 74; Stark & Z����iga 2014: 72; Derka & Figueroa 2016: 346. Material examined. BRA, RR, Pacaraima, Igarap�� Sam��, 04��28���30.08������N / 61��09���44.04������W, 10.x.2015, Pennsylvania trap, 1 female, R. Boldrini. (UFRR). BRA, RR, Pacaraima, 3�� Pelot��o Especial de Fronteira���PEF, Pista de Pouso, 04��49���37.83������N / 61��10���04.81������W, 10.vi.2016, light trap, 1 male, R. Boldrini, P. C. S. Barroso & W. S. Zeferino (UFRR). Measurements data. Female forewing length: 12.7���13.0 mm, n= 1; male forewing length: 8.5���9.0 mm, n= 1. Remarks. The specimens listed above have a head pattern and penis shape similar to those presented by Derka & Figueroa (2016). The single male listed above has a yellow head with a brown patch around the ocellus and a small spot on the clypeus, whereas the female lacks the clypeal spot. In the male, the wing venation is darker than the wing membrane. In the single female listed above, the wing venation is darker and the wing membrane more hyaline. Anacroneuria cruza was previously known from Venezuela (Derka & Figueroa 2016) and our Roraima State specimens are a new country record for Brazil., Published as part of Menezes, Emely Trajano De, Boldrini, Rafael & Novaes, Marcos Carneiro, 2020, New species of the genus Macrogynoplax and new records of Anacroneuria (Plecoptera: Perlidae) from Roraima State, Brazil, pp. 463-471 in Zootaxa 4722 (5) on page 466, DOI: 10.11646/zootaxa.4722.5.4, http://zenodo.org/record/3609700, {"references":["Stark, B. P. (1995) New species and records of Anacroneuria (Klapalek) from Venezuela (Insecta, Plecoptera, Perlidae). Spixiana, 18 (3), 211 - 249.","Stark, B. P. (1999) Anacroneuria from northeastern South America (Insecta: Plecoptera: Perlidae). Proceedings of the Biological Society of Washington, 112 (1), 70 - 93.","Stark, B. P. & Zuniga, M. C. (2014) New species and records of Colombian, Ecuadorian and Venezuelan Anacroneuria (Plecoptera: Perlidae), with a review of the Anacroneuria Aymara Stark & Sivec Complex. Illiesia, 10 (8), 66 - 79. Available from: http: // www 2. pms-lj. si / illiesia / Illiesia 10 - 08. pdf (accessed 16 November 2018)","Derka, T. & Figueroa, J. M. T. (2016) Contribution to the knowledge on habitat, distribution and morphological variability of two species of Anacroneuria Klapalek, 1909 (Plecoptera, Perlidae) from Venezuela. Bolentin de la Asociacion espanola de Entomologia, 40 (3 - 4), 341 - 349."]}

Anacroneuria marlieri Froehlich, 2001 Froehlich 2001: 191; Froehlich 2003: 130; Ribeiro & Rafael 2009: 7���8; Ribeiro & Gorayeb 2014: 18; Ribeiro & Gorayeb 2016: 434; Rippel et al. 2019: 360. Material examined. BRA, RR, Amajari, Serra do Tepequ��m, Igarap�� Cocal, 03��45���12.00���N / 61��42���42.00���W, 14.xii.2015, light trap, 1 female, R. Boldrini (UFRR); 14���29.xii.2015, Malaise trap, 1 female, R. Boldrini. (UFRR); 01���15.i.2016, Malaise trap, 5 male and 1 female, R. Boldrini. (UFRR); 02���16.i.2017, Malaise trap, 1 female, R. Boldrini (UFRR); 01���15.ii.2017, Malaise trap, 1 male and 1 female, R. Boldrini (UFRR); 03���13.x.2017, Malaise trap, 11 males and 4 females, (R. Boldrini. (UFRR); 13���23.x.2017, Malaise trap, 9 males and 3 females, R. Boldrini (UFRR); 03���13.xi.2017, Malaise trap, 12 males and 5 females, R. Boldrini (UFRR); 13���23.xi.2017, Malaise trap, 11 males and 4 females, R. Boldrini (UFRR); 23.xi���03.xii.2017, Malaise trap, 2 males and 1 female, R. Boldrini (UFRR). BRA, RR, Pacaraima, Igarap�� Miang, 04��29���09.45������N / 61��08���03.13������W, 04.xi.2016, light trap, 7 females, R. Boldrini. (UFRR). BRA, RR, Pacaraima, Igarap�� Sam��, 04��28���30.08������N 61��09���44.04������W, 10.x.2015, light trap, 1 male, R. Boldrini (UFRR). Measurements data. Female forewing length: 9.0��� 13.2 mm, n= 27; male forewing length: 8.1���11.0 mm, n= 43. Measurements data. A. marlieri variant. Male forewing length: 8.0 mm, n= 1. Remarks. Among the above material studied, a variant morphotype of A. marlieri was collected from Igarap�� Sam��. The single male is similar in size to males described by Rippel et al. (2019) but smaller than specimens reported by Ribeiro & Rafael (2009). There were also differences in the penis armature (0.07 mm length and 0.03���0.04 mm width) hammer shape which is conical and acute, and the hooks are curved down with acute apices. Additionally, there is a pointed triple dorsal keel and small membranous vesicles in ventral view. Anacroneuria marlieri was previously recorded from Amazonas, Par��, Rond��nia and Tocantins states of Brazil and our material is the first record for Roraima State., Published as part of Menezes, Emely Trajano De, Boldrini, Rafael & Novaes, Marcos Carneiro, 2020, New species of the genus Macrogynoplax and new records of Anacroneuria (Plecoptera: Perlidae) from Roraima State, Brazil, pp. 463-471 in Zootaxa 4722 (5) on page 468, DOI: 10.11646/zootaxa.4722.5.4, http://zenodo.org/record/3609700, {"references":["Froehlich, C. G. (2001) Guaranyperla, a new genus in the Gripopterygidae (Plecoptera). In: Dominguez, E. (Ed.), Trends in Research in Ephemeroptera and Plecoptera. Kluwer Academic / Plenum Publisher, New York, pp. 377 - 383. https: // doi. org / 10.1007 / 978 - 1 - 4615 - 1257 - 8 _ 44","Froehlich, G. C. (2003) Stoneflies (Plecoptera: Perlidae) from the Brazilian Amazonia with the description of three new species and a key to Macrogynoplax. Studies on Neotropical Fauna and Environment, 38 (2), 129 - 134. https: // doi. org / 10.1076 / snfe. 38.2.129.15929","Ribeiro, J. M. F. & Rafael, J. A. (2009) Redescription of the species of Anacroneuria Klapalek known from the Reserva Florestal Adolpho Ducke, Amazonas, Brazil with a neotype designation, and a key to adults males, Amazonas, Brazil. (Plecoptera, Perlidae). Zootaxa, 2004 (1), 1 - 15. https: // doi. org / 10.11646 / zootaxa. 2004.1.1","Ribeiro, J. M. F. & Gorayeb, I. S. (2014) Description of immatures and association with adults of three species of Anacroneuria Klapalek (Plecoptera: Perlidae) of the Brazilian Amazon. Zootaxa, 3881 (1), 17 - 32. https: // doi. org / 10.11646 / zootaxa. 3881.1.2","Ribeiro, J. M. F. & Gorayeb, I. S. (2016) Key to Plecoptera nymphs from the Brazilian Amazon (Insecta). Zootaxa, 4208 (5), 433 - 445. https: // doi. org / 10.11646 / zootaxa. 4208.5.2"]}

Tupiperla Froehlich, 1969 Paragripopteryx Illies, 1963 (nec Enderlein, 1909): 178. Tupiperla Froehlich, 1969: 28 (Type species: Semblis gracilis Burmeister, 1839, by monotypy); McLellan, 1977: 121 (in Gripopteryginae); Froehlich, 1998: 34; Stark, Froehlich & Zu��iga, 2009: 96; Froehlich, 2010: 137. Diversity. Tupiperla has 25 species described, including the five described in this paper (Table 1). Diagnosis. Tupiperla is diagnosed by: adults with a disto-ventral spine on each femur (Froehlich, 1969); forewing pattern with most crossveins with broad, squarish, and darker borders, forewing pterostigmatic crossveins absent; in males, the lack of a sclerotized epiproct, and a relatively long extension of tergum 10 (T10) that ends in two separate teeth (Bispo & Froehlich, 2007). The nymphs are slender; the femur, as in adults, is provided with a disto-ventral spine; the femur and tibia lack a dorsal row of long hairs (Froehlich, 1969)., Published as part of Duarte, T��cio, Novaes, Marcos Carneiro & Bispo, Pit��goras Da Concei����o, 2019, Five new species of Tupiperla Froehlich, 1969 (Plecoptera: Gripopterygidae), pp. 511-526 in Zootaxa 4671 (4) on page 513, DOI: 10.11646/zootaxa.4671.4.3, http://zenodo.org/record/3450478, {"references":["Froehlich, C. G. (1969) Studies on Brazilian Plecoptera 1. Some Gripopterygidae from the Biological Station at Paranapiacaba, State of Sao Paulo. Beitrage zur Neotropischen Fauna, 6, 17 - 39. https: // doi. org / 10.1080 / 0165052690936041 2","Illies, J. (1963) Revision der sudamerikanischen Gripopterygidae (Plecoptera). Mitteilungen der Schweizerischen Entomologischen Gesellschaft, 36, 145 - 248.","Enderlein, G. (1909) Klassification der Plecopteren sowie Diagnosen neuer Gattungen und Arten. Zoologischer Anzeiger, 34, 385 - 419.","Burmeister, H. (1839) Handbuch der Entomologie, Band 2. G. Reimer, Berlin, pp. 863 - 881.","McLellan, I. D. (1977) New alpine and southern Plecoptera from New Zealand, and a new classification of the Gripopterygidae. New Zealand Journal of Zoology, 4, 119 - 147. https: // doi. org / 10.1080 / 03014223.1977.9517945","Froehlich, C. G. (1998) Seven new species of Tupiperla (Plecoptera: Gripopterygidae) from Brazil, with a revision of the genus. Studies on Neotropical Fauna and Environment, 33, 19 - 36. https: // doi. org / 10.1076 / snfe. 33.1.19.2170","Froehlich, C. G. (2010) Catalogue of Neotropical Plecoptera. Illiesia, 6 (12), 118 - 205.","Bispo, P. C. & Froehlich, C. G. (2007) Stoneflies (Plecoptera) from northern Goias State, central Brazil: new record of Kempnyia oliveirai (Perlidae) and a new species of Tupiperla (Gripopterygidae). Aquatic Insects, 29, 213 - 217. https: // doi. org / 10.1080 / 01650420701411184"]}

Tupiperla pinhoi sp. nov. (Figs. 2 A���B, Figs. 3 A���C) Material Examined. Holotype. Male. Brazil: Santa Catarina: Urubici, Cachoeira do Avencal, 1229 m a.s.l., S28��02.798, W49��37.070, 08.i.2010, net, HF Mendes, LC Pinho & RA Moretto col. (MZSP). Measurement. Holotype, male: head width, 1.0 mm; pronotum width, 0.83 mm; pronotum length, 0.64 mm; forewing length, 7.9 mm; hind wing length, 6.7 mm; antenna length, 8.1 mm; 15 cercomeres. Description. Tupiperla pinhoi sp. nov. is a medium sized species. General color brownish to ochraceous. Head brownish with a lighter area in front of the coronal fork, and laterally to the eyes (Fig. 2A); ocelli and eyes black; anterior part of frons brown; labrum light brown, darker than clypeus; lateral part of the head, behind the eyes, with a dark brown band. Maxillary and labial palpi ochraceous. Antenna light brown. Pronotum squarish, light brown, narrower than the head and with some little setae covering it (Fig. 2A). Legs light brown, covered with thin bristles; femur with disto-ventral spine; tibia with two distal spurs; tarsi with tarsomere 1 medium, tarsomere 2 short, and tarsomere 3 long. Wings membranous, light brown; forewing with a conspicuous darker pattern bordering veins and crossveins; pterostigmatic crossvein absent, RA unforked, RP forked and CuA long-forked; hind wing with M3+4, near its separation from M1+2, fused with CuA in part of its length, CuA short forked, 6th anal vein fused with hind margin of wing (Fig. 2B). ARG, Argentina (MIS, Misiones Province); BRA, Brazil (BA, Bahia State; ES, Esp��rito Santo State; GO, Goi��s State; MG, Minas Gerais State; PR, Paran�� State; PE, Pernambuco State; RJ, Rio de Janeiro State; RS, Rio Grande do Sul State; SC, Santa Catarina State; SP, S��o Paulo State); PRY, Paraguay (PRI, Paraguar�� Department);?, when Froehlich (1969) described nymph of T. gracilis, the genus was considered monotypic; however, according to Froehlich (1998), it is likely that the nymph described in 1969 belongs to another species of the genus. Male. Abdomen brownish to ochraceous with slightly lighter band on abdominal terga 1���9. T10 light brown to ochraceous. T10 extension, in dorsal view, large, distally darker, ending in two separated and out curved teeth (Fig. 3A); in lateral view, straight dorsally and with a clear latero-ventral band (Fig. 3C). Paraprocts, in ventral view, thin, medially curved with thin bristles internally and conspicuous minute teeth on dorsal (outer) margin (Fig. 3B); in lateral view, with constant width over most of its length, dorsal margin darker and medially covered with minute teeth, distal region with an accentuated hook-shaped curvature (Fig. 3C). Subgenital plate ochraceous, ovoid-shaped and with apex prolonged between the paraprocts (Fig. 3B). Epiproct absent. Female and nymph. Unknown. Remarks. Tupiperla pinhoi can be distinguished from the congeners by the shape of the paraprocts and the T10 extension in males. The median dorsal margin of the paraprocts in T. pinhoi are covered by minute teeth, and the distal region has a distinct hook-shaped curvature. Only T. illiesi, T. reichardti Froehlich, 1998, and T. serrulata sp. nov. have minute teeth on the dorsal margin of the paraprocts. However, in T. illiesi each paraproct has a small and thin latero-distal spine, whereas in T. reichardti, each paraproct has a large latero-medial spine and in T. serrulata sp. nov., the paraprocts lack a lateral spine. Tupiperla pinhoi was collected in 2010 at the Avencal Waterfall, at 1229 m a.s.l. in the municipality of Urubici, Santa Catarina, Brazil. The waterfall is 100 m high and it formed by the Funil River. The common vegetation in the area is the ���avenca��� (Pteridaceae: Adiantum sp.). Etymology. The specific name pinhoi honors Prof. Dr. Luiz Carlos de Pinho for his contribution to aquatic entomology, especially related to Brazilian chironomids., Published as part of Duarte, T��cio, Novaes, Marcos Carneiro & Bispo, Pit��goras Da Concei����o, 2019, Five new species of Tupiperla Froehlich, 1969 (Plecoptera: Gripopterygidae), pp. 511-526 in Zootaxa 4671 (4) on pages 513-517, DOI: 10.11646/zootaxa.4671.4.3, http://zenodo.org/record/3450478, {"references":["Bispo, P. C. & Lecci, L. S. (2011) Gripopterygidae (Plecoptera) from Paranapiacaba mountains, southeastern Brazil. Annales de Limnologie - International Journal of Limnology, 47, 373 - 385. https: // doi. org / 10.1051 / limn / 2011052","Froehlich, C. G. (2016) Tupiperla (Plecoptera: Gripopterygidae) from southwestern Minas Gerais State, Brazil, with the description of Tupiperla amorimi n. sp. Zootaxa, 4103 (2), 174 - 176. http: // doi. org / 10.11646 / zootaxa. 4103.2.7","Duarte, T., Lecci, L. S. & Calor, A. R. (2014 b) Stoneflies (Insecta: Plecoptera) from Serra Bonita, Bahia, Brazil: new species and updated records for Northeastern. Zootaxa, 3779 (1), 81 - 92. https: // doi. org / 10.11646 / zootaxa. 3779.1.9","Froehlich, C. G. (1994) Brazilian Plecoptera 8. On Paragripopteryx (Gripopterygidae). Aquatic Insects, 16, 227 - 239. https: // doi. org / 10.1080 / 01650429409361559","Froehlich, C. G. (2002) Two New Species of Tupiperla (Plecoptera: Gripopterygidae) from the Missions Area of Argentina and Paraguay. Aquatic Insects, 24, 37 - 40. https: // doi. org / 10.1076 / aqin. 24.1.37.4906","Burmeister, H. (1839) Handbuch der Entomologie, Band 2. G. Reimer, Berlin, pp. 863 - 881.","Duarte, T., Bispo, P. C. & Calor, A. R. (2014 a) A new species of Tupiperla Froehlich, 1969 (Plecoptera: Gripopterygidae) from the Serra da Jiboia, Bahia, Brazil. Zootaxa, 3835 (1), 140 - 144. https: // doi. org / 10.11646 / zootaxa. 3835.1.9","Froehlich, C. G. (1998) Seven new species of Tupiperla (Plecoptera: Gripopterygidae) from Brazil, with a revision of the genus. Studies on Neotropical Fauna and Environment, 33, 19 - 36. https: // doi. org / 10.1076 / snfe. 33.1.19.2170","Bispo, P. C. & Froehlich, C. G. (2007) Stoneflies (Plecoptera) from northern Goias State, central Brazil: new record of Kempnyia oliveirai (Perlidae) and a new species of Tupiperla (Gripopterygidae). Aquatic Insects, 29, 213 - 217. https: // doi. org / 10.1080 / 01650420701411184","Novaes, M. C. & Bispo, P. C. (2016) A New species and records of Gripopterygidae (Plecoptera) from Rio Grande do Sul State, Southern Brazil. Zootaxa, 4175 (5), 487 - 490. https: // doi. org / 10.11646 / zootaxa. 4175.5. 7","Brauer, F. (1868) Familie Perlidae. In: Brauer, F. (Ed.), Neuropteren. Reise der Osterreichischen Fregatte Novara um die Erde in den Jahren 1857, 1858, 1859. Zoologischer Theil, 2. Band (1: A), 4, pp. 51 - 52.","Froehlich, C. G. (1969) Studies on Brazilian Plecoptera 1. Some Gripopterygidae from the Biological Station at Paranapiacaba, State of Sao Paulo. Beitrage zur Neotropischen Fauna, 6, 17 - 39. https: // doi. org / 10.1080 / 0165052690936041 2"]}

Tupiperla pessacqi sp. nov. (Figs. 2 E–F, Figs. 5 A–E) Material Examined. Holotype. Male. Brazil: Santa Catarina: Urubici, Cachoeira Avencal, 1229m a.s.l., S28°02.798, W49°37.070, 08.i.2010, light trap, HF Mendes, LC Pinho & RA Moretto col. (MZSP). Paratypes. Same data as holotype, except for: 4 females; same data, except for: 1 female; same data, except for: Cachoeira Véu de Noiva – 1350m a.s.l., S28°07.380, W49°28.808, 09.i.2010, 1 male, 1 female (UFBA); same data, except for: S28°01’51’’, W49°36’01’’, LC Pinho & AP Amaral col., 1 male; same data, except for: São Joaquim National Park, Rio Italianinho, Cachoeira, S28°08’49’’, W49°37’06’’, 05.ix-07.x.2014, Malaise trap, LC Pinho et al. col., 1 male; Rio da Serra, 12.xii, light trap, 1 male; Brazil: Santa Catarina: Grão Pará, Rio Braço Esquerdo, S28°09’01’’, W49°21’21’’, 16.xi.2012, light trap, LC Pinho, LC Gomes & AL Schlindwein col., 1 male; same data, except for: S28°07’48’’, W49°25’25’’, 7.iv-7.v.2013, Malaise, LC Pinho & V Freitas col., 1 female; same data, except for: Rio Três Barras, S28°12’44’’, W49°27’28’’, 15.xi.2012, light trap, LC Pinho, LC Gomes & AL Schlindwein col., 1 female. Measurement. Holotype, male: head width, 1.0 mm; pronotum width, 0.8 mm; pronotum length, 0.67 mm; forewing length, 8.3 mm; hind wing length, 7.2 mm; 15 cercomeres. Paratypes: Male (n= 5): head width, 0.98–1.0 mm; pronotum width, 0.7–0.8 mm; pronotum length, 0.64–0.67 mm; forewing length, 8.2–8.3 mm; hind wing length, 7.1–7.2 mm; 15–16 cercomeres. Female (n= 8): head width, 1.1 mm; pronotum width, 0.9 mm; pronotum length, 0.68–0.8 mm; forewing length, 9.8–10.0 mm; hind wing length, 8.3–8.4 mm; 14–15 cercomeres. Description. Tupiperla pessacqi sp. nov. is a small sized species. General color brown. Head brown with lighter area in front of the coronal fork, and laterally to the eyes (Fig. 2E); ocelli and eyes black; anterior part of frons darker; labrum brown, darker than clypeus; lateral part of the head, behind the eyes, with a dark brown band. Maxillary palpus brown; labial palpus light brown. Antenna brown. Pronotum brown, square with rounded corners, and narrower than the head (Fig. 2E). Legs dark brown, covered with thin bristles; femur lighter proximally and darker distally and with disto-ventral spine present; tibia and tarsi dark brown; tibia with two spurs distally; tarsi with tarsomere 1 medium, tarsomere 2 short, and tarsomere 3 long. Wings membranous, brown; forewing with a weak darker pattern bordering veins and crossveins, pterostigmatic crossvein absent, RA unforked, RP forked and CuA long forked; hind wing with M3+4, near its separation from M1+2, fused with CuA in part of its length, CuA short forked, 6th anal vein fused with hind margin of wing (Fig. 2F). Cercomeres covered by conspicuous small bristles. Male. Abdomen brown, with a conspicuous inverted trapezium-shaped clear band on abdominal terga 1–9. T10 dark brown. T10 extension, in dorsal view, elongated and thin, ending in two teeth, which are close to each other (Fig. 5A); in lateral view, straight dorsally, ending in two downcurved teeth (Fig. 5C). Paraprocts, in ventral view, thin, and with apex twisted (Fig. 5B); in lateral view, with middle region broad, apex truncated and up curved (Fig. 5C). Subgenital plate brownish with apex triangular, and prolonged between the paraprocts (Fig. 5B). Epiproct absent. Female. Abdominal segments 1–7 lighter; St 7 with two conspicuous sclerites. St8 forms a sclerotized subgenital plate, rectangle-shaped and with rounded corners; proximal margin of the plate where it meets St7 strongly sclerotized; apex of the plate reaching St9, but not surpassing it (Figs. 5 D–E). Paraprocts short, and with apex slightly truncated (Figs. 5 D–E). Several thin bristles covering the St8–10 and paraprocts. Nymphs. Unknown. Remarks. Tupiperla pessacqi can be distinguished from the congeners by the shape of paraprocts in males and by the subgenital plate of female. The paraprocts of T. pessacqi resembles those of T. illiesi, however, in the latter species, each paraproct has a subapical lateral spine, whereas in T. pessacqi the distal region is twisted. In addition, the paraprocts of T. illiesi are covered by minute teeth on the dorsal margin, absent in T. pessacqi. The subgenital plate of female of the both species are distinctive. In T. pessacqi the subgenital plate is square-shaped, whereas in T. illiesi, the plate is truncate to oblong with small posterolateral extensions. Tupiperla pessacqi is described based on male and female specimens collected in the municipalities of Urubici and Grão Pará, Santa Catarina State, Brazil. Most of the material comes from Avencal (including the holotype) and Véu de Noiva waterfalls in the São Joaquim National Park in Urubici. Additional material was collected in Serra River in Urubici, and Braço Esquerdo and Três Barras rivers in Grão Pará. Etymology. The specific name pessacqi honors Prof. Dr. Pablo Pessacq, Argentina, for his contribution on taxonomy of aquatic entomology, especially related to Odonata and Plecoptera.

Tupiperla ubuntu sp. nov. (Figs. 2 G–H, Figs. 6 A–E) Material Examined. Holotype. Male. Brazil: Santa Catarina: Orleans, Rio Minador, S28°10’24’’, W49°24’37’’, 19.viii-12.x.2013, Malaise trap, LC Pinho et al. col. (MZSP). Paratypes. Same data, except for: S28°10’28’’, W49°24’36’’, 10.xi-13.xii.2013, Malaise trap, LC Pinho et al. col., 3 males, 24 females; same data, except for: 12.x-10.xi.2013, Malaise trap, LC Pinho et al. col., 22 females; same data, except for: 14.viii-12.x.2013, Malaise trap, LC Pinho et al. col., 1 male, 26 females (1 male, 2 females at UFBA); same data, except for: 06.vii-19.viii.2013, Malaise trap, LC Pinho et al. col., 3 females; same data, except for: 19.viii-12.x.2013, Malaise trap, LC Pinho et al. col., 1 male, 25 females; same data, except for: S28°10’24’’, W49°24’37’’, 13.xii.2013 - 11.i.2014, Malaise trap, LC Pinho et al. col., 1 female; same data, except for: S28°10’35’’, W49°24’36’’, 12.x-10.xi.2013, Malaise trap, LC Pinho et al. col., 1 female; same data, except for: S28°10’43’’, W49°24’33’’, 07.v-08.vi.2013, Malaise trap, LC Pinho, AP Amaral, MO Bessel & AM Will col., 1 male; same data, except for: Rio Três Barras, S28°12’44’’, W49°27’28’’, 15.x.2012, light trap, LC Pinho, LS Gomes & AL Schlindwein col., 1 male; Malaise trap, ilha, 12.xii.2013, 1 female; #78, 2 females. Measurement. Holotype, male: head width, 0.9 mm; pronotum width, 0.8 mm; pronotum length, 0.62 mm; forewing length, 7.3 mm; hind wing length, 6.0 mm; antenna length, 7.3 mm; 15 cercomeres. Paratypes: Males (n= 7): head width, 0.9–1.0 mm; pronotum width, 0.74–0.8 mm; pronotum length, 0.55–0.8 mm; forewing length, 7.3–7.6 mm; hind wing length, 6.2–6.5 mm; antenna length, 7.2–7.3 mm; 13–15 cercomeres. Females (n= 105): head width, 1.0– 1.1 mm; pronotum width, 0.9–0.94 mm; pronotum length, 0.73–0.75 mm; forewing length, 9.2–9.4 mm; hind wing length, 8.0– 8.1 mm; antenna length, 7.4 mm; 12–16 cercomeres. Description. Tupiperla ubuntu sp. nov. is a small sized species. General color brown. Head dark brown with lighter area in front of the coronal fork, and laterally to the eyes (Fig. 2G); ocelli and eyes black; anterior part of frons darker; clypeus and labrum brown; lateral part of the head, behind eyes, with a distinct dark brown band. Maxillary and labial palpi light brown. Antenna dark brown. Pronotum squarish, with rounded corners, and narrower than the head (Fig. 2G). Legs dark brown; femur lighter proximally and darker distally, covered with thin bristles, and with disto-ventral spine present; tibia and tarsi darker, and covered with thin bristles. Wings membranous, brownish; forewing with darker patterns bordering veins and crossveins conspicuous, pterostigmatic crossveins absent, RA unforked, RP forked and CuA long forked; hind wing with M3+4, near its separation from M1+2, fused with CuA in part of its length, CuA long forked, 6th anal vein fused with hind margin of wing (Fig. 2H). Male. Abdomen brown, with a conspicuous inverted trapezium-shaped clear band on abdominal terga 1–9. T10 dark brown. T10 extension almost black; in dorsal view, fish tail shaped (Fig. 6A); in lateral view, ending in two large downcurved teeth (Fig. 6C). Paraprocts, in ventral view, thin, apex spoon-shaped, and with several external median bristles (Fig. 6B); in lateral view, narrow at the base, expanding toward a truncated apex, which has a subapical dorsal tooth (Fig. 6C). Subgenital plate brownish with central area darker, triangular shaped and with apex prolonged between the paraprocts (Fig. 6B). Epiproct absent. Female. Abdominal segments 1–7 milky; St7 with two-minute sclerites. Several thin bristles covering St8–10 and paraprocts. St8 forms a long sclerotized subgenital plate that reaches St10, but do not surpass it; plate with a conspicuous lateral narrowing near the base, gradually widening from the base to the apex (Figs. 6 D–E). Paraprocts, in lateral view, elongated, apex pointed (Fig. 6E). Nymphs. unknown. Remarks. Males of Tupiperla ubuntu has a T10 extension that resembles that of T. oliveirai. However, the paraprocts in T. ubuntu are thin and expand gradually towards a truncated apex, ending postero-dorsally in a small pointed tooth. In T. oliveirai, the paraprocts are strongly dilated, bent upward in last third, with a membranous area apically and a subapical dorsal tooth. Tupiperla ubuntu is described based on male and female specimens collected in the village of Minador River in the municipality of Orleans, Santa Catarina State, Brazil. Most material was collected using Malaise traps from August to December 2013. The number of females collected was 15 times greater than the number of males. Etymology. The specific name ubuntu is in allusion to the ancient African word (Nguni word) which brings the notion of solidarity, cooperation, respect, and generosity. The name is a noun in apposition.

Tupiperla zwicki sp. nov. (Figs. 2 I–J, Figs. 7 A–C) Material Examined. Holotype. Male. Brazil: Santa Catarina: Orleans, Rio Minador, S28°10’28’’, W49°24’36’’, 14.viii-12.x.2013, Malaise trap, LC Pinho et al. (MZSP). Paratypes. Same data as holotype, except for: 1 male; same data, except for: 10.xi-13.xii.2013, Malaise trap, LC Pinho et al., 1 male; Brazil: Santa Catarina: Urubici, RPPN Portal das Nascentes, S28°03’12’’, W49°22’27’’, 19.viii-12.x.2013, Malaise trap, LC Pinh o et al., 1 male (at UFBA); same data, except for: Pousada N.S. Graças, S27°59.762, W49°34.040, 06-10.i.2010, HF Mendes, LC Pinho & RA Moretto col., 1 male. Measurement. Holotype, male: head width, 0.93 mm; pronotum width, 0.78 mm; pronotum length, 0.61 mm; forewing length, 7.5 mm; hind wing length, 6.4 mm; 14 cercomeres. Paratypes: Males (n= 4): head width, 0.97–1.2 mm; pronotum width, 0.7–0.9 mm; pronotum length, 0.6–0.8 mm; forewing length, 8.1–9.4 mm; hind wing length, 6.8–8.2 mm; 13–14 cercomeres. Description. Tupiperla zwicki sp. nov. is a medium sized species. General color brownish to ochraceous. Head brownish with a lighter area in front of the coronal fork, and laterally to the eyes; ocelli and eyes black (Fig. 2I); clypeus and labrum brownish; lateral part of the head, behind the eyes, with a distinct dark brown band. Maxillary and labial palpi ochraceous. Antenna brown. Pronotum squarish, slightly trapezoidal, light brown, with latero-anterior corners rounded, and narrower than the head (Fig. 2I). Legs light brown and covered by thin bristles; femur with disto-ventral spine present; tibia with two spurs at the distal region; tarsi with tarsomere 1 medium, tarsomere 2 short, and tarsomere 3 long. Wings membranous, light brown; forewing with weak darker patterns bordering veins and crossveins, pterostigmatic crossvein absent, RA unforked, RP forked and CuA long forked; hind wing with M3+4, near its separation from M1+2, fused with CuA in part of its length, CuA short forked, 6th anal vein fused with hind margin of wing (Fig. 2J). Male. Abdomen brownish to ochraceous, and with paler band on abdominal terga 1–9. T10 light brown to ochraceous; laterally covered with thin bristles. T10 extension, in dorsal view, large, and with apex bifurcated (Yshaped) (Fig. 7A); in lateral view, straight dorsally, distally downcurved and ending in two large teeth (Fig. 7C). Paraprocts, in ventral view, thin, covered with thin bristles, and with distal region out curved (Fig. 7B); in lateral view, thin on base and broadening softly along the length, with ventral margin almost straight, dorsal margin slightly sinuous and darker, and apex rounded (Fig. 7C). Subgenital plate ochraceous, and ovoid-shaped (Fig. 7B). Epiproct absent. Female and nymphs. Unknown. Remarks. Tupiperla zwicki can be distinguished from the congeners by the shape of the paraprocts and T10 extension in males. In lateral view, T. zwicki resembles T. illiesi, however, in this species, each paraproct has a row of small teeth on dorsal margin, and the latero-distal region has a spine, while in T. zwicki there are no teeth in the dorsal margin of the paraprocts, and the distal portion is curved outwards. Tupiperla zwicki is described based on specimens collected in municipalities of Orleans and Urubici, Santa Catarina State, Brazil. Four male specimens (including the holotype) were collected from August to January 2013, another male was collected in January 2010. Several females were collected in the same locality but are left unassociated. Etymology. The specific name zwicki honors Prof. Dr. Peter Zwick, Schlitz, Germany for his numerous contributions to aquatic entomology, especially on the taxonomy and phylogeny of the Plecoptera.

Tupiperla zwicki sp. nov. (Figs. 2 I���J, Figs. 7 A���C) Material Examined. Holotype. Male. Brazil: Santa Catarina: Orleans, Rio Minador, S28��10���28������, W49��24���36������, 14.viii-12.x.2013, Malaise trap, LC Pinho et al. (MZSP). Paratypes. Same data as holotype, except for: 1 male; same data, except for: 10.xi-13.xii.2013, Malaise trap, LC Pinho et al., 1 male; Brazil: Santa Catarina: Urubici, RPPN Portal das Nascentes, S28��03���12������, W49��22���27������, 19.viii-12.x.2013, Malaise trap, LC Pinh o et al., 1 male (at UFBA); same data, except for: Pousada N.S. Gra��as, S27��59.762, W49��34.040, 06-10.i.2010, HF Mendes, LC Pinho & RA Moretto col., 1 male. Measurement. Holotype, male: head width, 0.93 mm; pronotum width, 0.78 mm; pronotum length, 0.61 mm; forewing length, 7.5 mm; hind wing length, 6.4 mm; 14 cercomeres. Paratypes: Males (n= 4): head width, 0.97���1.2 mm; pronotum width, 0.7���0.9 mm; pronotum length, 0.6���0.8 mm; forewing length, 8.1���9.4 mm; hind wing length, 6.8���8.2 mm; 13���14 cercomeres. Description. Tupiperla zwicki sp. nov. is a medium sized species. General color brownish to ochraceous. Head brownish with a lighter area in front of the coronal fork, and laterally to the eyes; ocelli and eyes black (Fig. 2I); clypeus and labrum brownish; lateral part of the head, behind the eyes, with a distinct dark brown band. Maxillary and labial palpi ochraceous. Antenna brown. Pronotum squarish, slightly trapezoidal, light brown, with latero-anterior corners rounded, and narrower than the head (Fig. 2I). Legs light brown and covered by thin bristles; femur with disto-ventral spine present; tibia with two spurs at the distal region; tarsi with tarsomere 1 medium, tarsomere 2 short, and tarsomere 3 long. Wings membranous, light brown; forewing with weak darker patterns bordering veins and crossveins, pterostigmatic crossvein absent, RA unforked, RP forked and CuA long forked; hind wing with M3+4, near its separation from M1+2, fused with CuA in part of its length, CuA short forked, 6th anal vein fused with hind margin of wing (Fig. 2J). Male. Abdomen brownish to ochraceous, and with paler band on abdominal terga 1���9. T10 light brown to ochraceous; laterally covered with thin bristles. T10 extension, in dorsal view, large, and with apex bifurcated (Yshaped) (Fig. 7A); in lateral view, straight dorsally, distally downcurved and ending in two large teeth (Fig. 7C). Paraprocts, in ventral view, thin, covered with thin bristles, and with distal region out curved (Fig. 7B); in lateral view, thin on base and broadening softly along the length, with ventral margin almost straight, dorsal margin slightly sinuous and darker, and apex rounded (Fig. 7C). Subgenital plate ochraceous, and ovoid-shaped (Fig. 7B). Epiproct absent. Female and nymphs. Unknown. Remarks. Tupiperla zwicki can be distinguished from the congeners by the shape of the paraprocts and T10 extension in males. In lateral view, T. zwicki resembles T. illiesi, however, in this species, each paraproct has a row of small teeth on dorsal margin, and the latero-distal region has a spine, while in T. zwicki there are no teeth in the dorsal margin of the paraprocts, and the distal portion is curved outwards. Tupiperla zwicki is described based on specimens collected in municipalities of Orleans and Urubici, Santa Catarina State, Brazil. Four male specimens (including the holotype) were collected from August to January 2013, another male was collected in January 2010. Several females were collected in the same locality but are left unassociated. Etymology. The specific name zwicki honors Prof. Dr. Peter Zwick, Schlitz, Germany for his numerous contributions to aquatic entomology, especially on the taxonomy and phylogeny of the Plecoptera., Published as part of Duarte, T��cio, Novaes, Marcos Carneiro & Bispo, Pit��goras Da Concei����o, 2019, Five new species of Tupiperla Froehlich, 1969 (Plecoptera: Gripopterygidae), pp. 511-526 in Zootaxa 4671 (4) on pages 522-524, DOI: 10.11646/zootaxa.4671.4.3, http://zenodo.org/record/3450478

Tupiperla ubuntu sp. nov. (Figs. 2 G���H, Figs. 6 A���E) Material Examined. Holotype. Male. Brazil: Santa Catarina: Orleans, Rio Minador, S28��10���24������, W49��24���37������, 19.viii-12.x.2013, Malaise trap, LC Pinho et al. col. (MZSP). Paratypes. Same data, except for: S28��10���28������, W49��24���36������, 10.xi-13.xii.2013, Malaise trap, LC Pinho et al. col., 3 males, 24 females; same data, except for: 12.x-10.xi.2013, Malaise trap, LC Pinho et al. col., 22 females; same data, except for: 14.viii-12.x.2013, Malaise trap, LC Pinho et al. col., 1 male, 26 females (1 male, 2 females at UFBA); same data, except for: 06.vii-19.viii.2013, Malaise trap, LC Pinho et al. col., 3 females; same data, except for: 19.viii-12.x.2013, Malaise trap, LC Pinho et al. col., 1 male, 25 females; same data, except for: S28��10���24������, W49��24���37������, 13.xii.2013 - 11.i.2014, Malaise trap, LC Pinho et al. col., 1 female; same data, except for: S28��10���35������, W49��24���36������, 12.x-10.xi.2013, Malaise trap, LC Pinho et al. col., 1 female; same data, except for: S28��10���43������, W49��24���33������, 07.v-08.vi.2013, Malaise trap, LC Pinho, AP Amaral, MO Bessel & AM Will col., 1 male; same data, except for: Rio Tr��s Barras, S28��12���44������, W49��27���28������, 15.x.2012, light trap, LC Pinho, LS Gomes & AL Schlindwein col., 1 male; Malaise trap, ilha, 12.xii.2013, 1 female; #78, 2 females. Measurement. Holotype, male: head width, 0.9 mm; pronotum width, 0.8 mm; pronotum length, 0.62 mm; forewing length, 7.3 mm; hind wing length, 6.0 mm; antenna length, 7.3 mm; 15 cercomeres. Paratypes: Males (n= 7): head width, 0.9���1.0 mm; pronotum width, 0.74���0.8 mm; pronotum length, 0.55���0.8 mm; forewing length, 7.3���7.6 mm; hind wing length, 6.2���6.5 mm; antenna length, 7.2���7.3 mm; 13���15 cercomeres. Females (n= 105): head width, 1.0��� 1.1 mm; pronotum width, 0.9���0.94 mm; pronotum length, 0.73���0.75 mm; forewing length, 9.2���9.4 mm; hind wing length, 8.0��� 8.1 mm; antenna length, 7.4 mm; 12���16 cercomeres. Description. Tupiperla ubuntu sp. nov. is a small sized species. General color brown. Head dark brown with lighter area in front of the coronal fork, and laterally to the eyes (Fig. 2G); ocelli and eyes black; anterior part of frons darker; clypeus and labrum brown; lateral part of the head, behind eyes, with a distinct dark brown band. Maxillary and labial palpi light brown. Antenna dark brown. Pronotum squarish, with rounded corners, and narrower than the head (Fig. 2G). Legs dark brown; femur lighter proximally and darker distally, covered with thin bristles, and with disto-ventral spine present; tibia and tarsi darker, and covered with thin bristles. Wings membranous, brownish; forewing with darker patterns bordering veins and crossveins conspicuous, pterostigmatic crossveins absent, RA unforked, RP forked and CuA long forked; hind wing with M3+4, near its separation from M1+2, fused with CuA in part of its length, CuA long forked, 6th anal vein fused with hind margin of wing (Fig. 2H). Male. Abdomen brown, with a conspicuous inverted trapezium-shaped clear band on abdominal terga 1���9. T10 dark brown. T10 extension almost black; in dorsal view, fish tail shaped (Fig. 6A); in lateral view, ending in two large downcurved teeth (Fig. 6C). Paraprocts, in ventral view, thin, apex spoon-shaped, and with several external median bristles (Fig. 6B); in lateral view, narrow at the base, expanding toward a truncated apex, which has a subapical dorsal tooth (Fig. 6C). Subgenital plate brownish with central area darker, triangular shaped and with apex prolonged between the paraprocts (Fig. 6B). Epiproct absent. Female. Abdominal segments 1���7 milky; St7 with two-minute sclerites. Several thin bristles covering St8���10 and paraprocts. St8 forms a long sclerotized subgenital plate that reaches St10, but do not surpass it; plate with a conspicuous lateral narrowing near the base, gradually widening from the base to the apex (Figs. 6 D���E). Paraprocts, in lateral view, elongated, apex pointed (Fig. 6E). Nymphs. unknown. Remarks. Males of Tupiperla ubuntu has a T10 extension that resembles that of T. oliveirai. However, the paraprocts in T. ubuntu are thin and expand gradually towards a truncated apex, ending postero-dorsally in a small pointed tooth. In T. oliveirai, the paraprocts are strongly dilated, bent upward in last third, with a membranous area apically and a subapical dorsal tooth. Tupiperla ubuntu is described based on male and female specimens collected in the village of Minador River in the municipality of Orleans, Santa Catarina State, Brazil. Most material was collected using Malaise traps from August to December 2013. The number of females collected was 15 times greater than the number of males. Etymology. The specific name ubuntu is in allusion to the ancient African word (Nguni word) which brings the notion of solidarity, cooperation, respect, and generosity. The name is a noun in apposition., Published as part of Duarte, T��cio, Novaes, Marcos Carneiro & Bispo, Pit��goras Da Concei����o, 2019, Five new species of Tupiperla Froehlich, 1969 (Plecoptera: Gripopterygidae), pp. 511-526 in Zootaxa 4671 (4) on pages 520-522, DOI: 10.11646/zootaxa.4671.4.3, http://zenodo.org/record/3450478

Tupiperla Froehlich, 1969 Paragripopteryx Illies, 1963 (nec Enderlein, 1909): 178. Tupiperla Froehlich, 1969: 28 (Type species: Semblis gracilis Burmeister, 1839, by monotypy); McLellan, 1977: 121 (in Gripopteryginae); Froehlich, 1998: 34; Stark, Froehlich & Zuñiga, 2009: 96; Froehlich, 2010: 137. Diversity. Tupiperla has 25 species described, including the five described in this paper (Table 1). Diagnosis. Tupiperla is diagnosed by: adults with a disto-ventral spine on each femur (Froehlich, 1969); forewing pattern with most crossveins with broad, squarish, and darker borders, forewing pterostigmatic crossveins absent; in males, the lack of a sclerotized epiproct, and a relatively long extension of tergum 10 (T10) that ends in two separate teeth (Bispo & Froehlich, 2007). The nymphs are slender; the femur, as in adults, is provided with a disto-ventral spine; the femur and tibia lack a dorsal row of long hairs (Froehlich, 1969).

Anacroneuria zantedeschia n. sp. Figs. 2 A���2G. Material examined. Holotype male: BRAZIL (BRA), Tocantins State (TO), municipality of Palmas, Taquaru���� do Porto, Vale do Vai Quem Quer, Ribeir��o S��o Jo��o (-10.394327, -48.132334), 13���14.xi.2017, light pan trap, TKK, (MZUSP). Paratypes: same locality, 13���14.xi.2017, light pan trap, 1 male; 14���15.xi.2017, light pan trap, 1 male, light trap, 2 males, (MZUSP). Same locality, 13���14.xi.2017, light pan trap, 1 male, Pennsylvania trap, 1 male, (INPA). Same locality, 13���14.xi.2017, light trap, 2 males; 14���15.xi.2017, Pennsylvania trap, 1 male, (CEUFT). Description. General color brownish. Head light brown with central part ochraceous; clypeal area setose; Mline slightly visible (Fig. 2A) but in some specimens with a brown outline; lappets light brown; gena and parietalia light brown; postfrontal line U-shaped; antenna dark brown with the closest part to scape light brown; scape dark brown; palpi brown. Pronotum, brown to dark brown with the median part lighter and margins dark brown covered with setae (Fig. 2A). Legs, femora light brown, tibia and tarsi darker. Wing membrane brownish and veins brown (Fig. 2B). Sternum IX with dark posterior strip (Fig. 2C). Cerci dark brown. Male. Forewing length 6.6���8.7 mm (n= 9). Hammer a truncate cone, wider than long (Fig. 2C). Penial armature with no membranous lobes (Fig. 2D); apical portion irregular and robust, projecting distinctly forward of the hooks. In dorsal view (Fig. 2E), large gonopore projecting ventrally, border expanding shortly; keel very large and Y-shaped; apical part narrowing gradually to median part, then widening again, being slightly wider at base. In ventral view (Fig. 2D), hooks regularly curved, concave ventrally, with no pointed tips and acute apically. In lateral view (Fig. 2F), apex expanded dorsally, a deep depression near the level of basal part of hooks. Presence of patch of small sparse sclerotized spines covering the surface of the lower apical part of the penial armature in dorsal and ventral views, also in ventral view in the middle of basal portion of penial armature and in apical part closest to the hooks (Fig. 2G). Female and Nymph. Unknown. Remarks. The apical structure of the penial armature of A. zantedeschia. resembles A. caraa De Ribeiro & Froehlich 2007. However, a major difference is the position of the gonopore, which projects ventrally, whereas in A. caraa it projects apically. Additionally, the wing length of A. caraa is longer, male forewing length 11.8 mm, and the hammer is more pointed than in A. zantedeschia. Etymology. ��� Zantedeschia��� refers to the genus of the South African arum lily flower, Zantedeschia aethiopica (L.) Spreng, which the spathe resembles the gonopore of penial armature of A. zantedeschia., Published as part of Rippel, Mellis Layra Soares, Novaes, Marcos Carneiro & Krolow, Tiago K��tter, 2019, First records of the genus Anacroneuria (Plecoptera: Perlidae) from Tocantins State, Brazil and description of a new species, pp. 355-364 in Zootaxa 4560 (2) on pages 356-359, DOI: 10.11646/zootaxa.4560.2.8, http://zenodo.org/record/2627721

Anacroneuria otafroehlichi Righi-Cavallaro & Lecci 2010 Figs. 4 A–4B. Anacroneuria otafroehlichi Righi-Cavallaro & Lecci 2010: 37. Lecci & Righi-Cavallaro 2017: 2. Material examined. BRA, TO, municipality of Palmas, Taquaruçú do Porto, Cachoeira do Evilson, Ribeirão Taquaruçú Grande, (-10.23193, -48.123289), light trap, 18–19.x.2017, MLSR, ASF, (MZUSP). Paratypes: same data as holotype, 1 female and 1 male, (MZUSP). BRA, TO, municipality of Palmas, Taquaruçú do Porto, Fazenda Buritizal, Córrego Buritizal, (-10.28667222, -48.05347222), Pennsylvania trap, 2 females, 28–29.iii.2018, TKK, (CEUFT); light trap, 2 females and 1 male, 27–28.iii.2018, TKK, (CEUFT), 1 female and 1 male, 28–29.iii.2018, TKK, (CEUFT); light pan trap, 6 females, 27–28.iii.2018, TKK, (CEUFT). BRA, TO, municipality of Palmas, Taquaruçú do Porto, Sambaíba, Córrego Sambaíba, (-10.3780388889, -48.1240111111), Pennsylvania trap, 1 female, 14–15.xi.2017, TKK, (CEUFT), 4 females, 15–16.xi.2017, TKK, (CEUFT). BRA, TO, municipality of Palmas, Taquaruçú do Porto, Roncadeira, Ribeirão Taquaruçuzinho, (-10.303639, -8.138861), Pennsylvania trap, 2 females and 2 males, 17–20.x.2017, MLSR, ASF, (CEUFT), 9 females and 4 males, 18–19.x.2017, MLSR, ASF, (CEUFT), 1 female and 1 male, 19–20.x.2017, MLSR, ASF, (CEUFT). BRA, TO, municipality of Palmas, Taquaruçú do Porto, Fazenda Encantada, Ribeirão Taquaruçú Grande, (-10.244856, -48.123867), Pennsylvania trap, 1 female, 29.viii.2017, TKK, (CEUFT); light trap, 1 female, 26–27.viii.2017, TKK, (CEUFT). BRA, TO, municipality of Palmas, Taquaruçú do Porto, Cachoeira do Evilson, Ribeirão Taquaruçú Grande, (-10.23193, - 48.123289), manually collected, 1 female, 26.ix.2017, TKK, (CEUFT); light trap, 1 female, 21–24.iii.2016, TKK, (CEUFT), 4 females and 1 male, 29–30.viii.2017, TKK, (CEUFT), 1 female, 29–30.viii.2017, TKK, (INPA), 16 females and 18 males, 18–19.x.2017, MLSR, ASF, (CEUFT), 2 males, 18–19.x.2017, MLSR, ASF, (INPA), 2 males, 18–19.x.2017, MLSR, ASF, (CLBA), 11 females and 21 males, 19–20.x.2017, MLSR, ASF, (CEUFT), 1 female, 19–20.x.2017, MLSR, ASF, (CLBA); light pan trap, 2 females, 30.viii.2017, TKK, (CEUFT), 6 females and 8 males, 19–22.ix.2017, TKK, (CEUFT), 1 female and 2 males, 19–20.x.2017, MLSR, ASF, (CEUFT). BRA, TO, municipality of Palmas, Taquaruçú do Porto, Lajeiro, Ribeirão Taquaraçuzinho, (-48.174056, -10.304975), Pennsylvania trap, 1 female, 27.viii.2017, TKK, (CEUFT). BRA, TO, municipality of Palmas, Taquaruçú do Porto, Vale do Vai Quem Quer, Ribeirão São João (-10.394327, -48.132334), Pennsylvania trap, 2 females, 13– 14.xi.2017, TKK, (CEUFT); Malaise trap, 1 female, 13–16.xi.2017, TKK, (CEUFT); light trap, 1 female and 1 male, 13–14.xi.2017, TKK, (CEUFT), 3 females and 1 male, 14–15.xi. 2 0 17, TKK, (CEUFT). Measurements data: Female forewing: 11.9–15 mm, n= 84; male forewing: 9–10.9 mm, n= 65. Description of female. Subgenital plate light brown, 4-lobed (Fig. 4A); lateral lobes larger than paramedial lobes; sublateral notch deeper than median notch. Sternum 8 and 9 with patches of hair. Egg elongate oval (Fig. 4B), 0.21 x 0.356. Remarks. Righi-Cavallaro & Lecci (2010) described this species from the municipality of Chapadão do Sul, Mato Grosso do Sul, Brazil. The penial armature agrees with original description (Righi-Cavallaro & Lecci 2010). However, in lateral view, dorsal portion nearest to the base of the penial armature studied here is concave, but indicated as convex in Righi-Cavallaro & Lecci (2010). Additionally, our specimens differ in size from those of Righi-Cavallaro & Lecci (2010) (male forewing length: 12.2–13.2 mm). This is the first record of A. otafroehlichi from Tocantins State and from northern Brazil.

Anacroneuria debilis (Pictet 1841) Perla (Perla) debilis Pictet 1841: 255. Anacroneuria debilis Zwick 1972: 1155; Zwick 1973: 486; Froehlich 2002: 76; Avelino-Capistrano et al. 2011: 59–63; Baldin et al. 2013: 392; Bispo et al. 2014: 592; Novaes & Bispo 2014a: 434; Novaes & Bispo 2014b: 459; Duarte & Lecci 2016: 293; Almeida & Duarte 2017: 481; Gonçalves et al. 2017: 146. Material examined. BRA, TO, municipality of Palmas, Taquaruçú do Porto, Fazenda Buritizal, Córrego Buritizal, (-10.28667222, -48.05347222), Pennsylvania trap, 1 male, 28–29.xi.2017, TKK, (CEUFT). BRA, TO, municipality of Palmas, Taquaruçú do Porto, Roncadeira, Ribeirão Taquaruçuzinho, (-10.303639, -8.138861), Pennsylvania trap, 2 males, 17–20.x.2017, MLSR, ASF, (CEUFT). BRA, TO, municipality of Palmas, Taquaruçú do Porto, Vale do Vai Quem Quer, Ribeirão São João (-10.394327, -48.132334), Pennsylvania trap, 8 females and 2 males, 13–14.xi.2017, TKK, (CEUFT), 1 female and 2 males, 15–16.xi. 2 0 17, TKK, (CEUFT); Malaise trap, 4 males, 13–16.xi.2017, TKK, (CEUFT); light trap, 4 females and 3 males, 13–14.xi.2017, TKK, (CEUFT), 1 female, 13–14.xi.2017, TKK, (INPA), 2 females and 6 males, 14–15.xi.2017, TKK, (CEUFT); light pan trap, 8 females and 2 males, 13–14.xi.2017, TKK, (CEUFT), 6 females and 12 males, 14–15.xi.2017, TKK, (CEUFT), 2 males, 14–15.xi.2017, TKK, (INPA), 1 female and 2 males, TKK, (CLBA), 2 females, 15–16.xi.2017, TKK, (CEUFT). Measurements data. Female forewing: 12.5–16.5 mm, n= 33; male forewing: 9.6–11.9 mm, n=38. Remarks. Specimens studied here are smaller (forewing length of male: 10–14.5 mm) and lighter than that studied by Baldin et al. (2013). Additionally, our specimens possess a yellow spot medially on the head and the Mline is not as distinct as that observed in specimens from other areas of Brazil. Even with such differences, due to wide distribution of A. debilis we consider these differences as intraspecific. Regarding the penial armature of this species, its apical portion in lateral view is similar to the illustration in Zwick (1972) and differs slightly from Baldin et al. (2013). This species is recorded to northeastern Argentina (provinces of Misiones and Entre Ríos), Paraguay, and southern to northeastern Brazil (Duarte & Lecci 2016). This is the first record of this species for Tocantins State., Published as part of Rippel, Mellis Layra Soares, Novaes, Marcos Carneiro & Krolow, Tiago Kütter, 2019, First records of the genus Anacroneuria (Plecoptera: Perlidae) from Tocantins State, Brazil and description of a new species, pp. 355-364 in Zootaxa 4560 (2) on page 359, DOI: 10.11646/zootaxa.4560.2.8, http://zenodo.org/record/2627721, {"references":["Zwick, P. (1972) Die Plecopteren Pictets und Burmeisters, mit Angaben uber weitere Arten (Insecta). Revue Suisse de Zoologie, 78 (4), 1123 - 1194.","Froehlich, C. G. (2002) Anacroneuria mainly from southern Brazil and northeastern Argentina (Plecoptera: Perlidae). Proceedings of the Biological Society of Washington, 115 (1), 75 - 107.","Baldin, C., Bispo, P. C. & Novaes, M. C. (2013) New species and records of Anacroneuria (Plecoptera: Perlidae) from Rio de Janeiro State, Brazil. Zootaxa, 3694 (4), 391 - 397. https: // doi. org / 10.11646 / zootaxa. 3694.4.7","Bispo, P. C., Costa. L. S. M. & Novaes, M. C. (2014) Two new species and a new record of Anacroneuria (Plecoptera: Perlidae) from Central Brazil. Zootaxa, 3779 (5), 591 - 596. https: // doi. org / 10.11646 / zootaxa. 3779.5.9","Duarte, T. & Lecci, L. S. (2016) New species and records of Anacroneuria (Plecoptera: Perlidae) from the northeastern semiarid region of Brazil. Zootaxa, 4079 (2), 291 - 300. http: // doi. org / 10.11646 / zootaxa. 4079.2.10","Goncalves M. C., Novaes, M. C. & Salles, F. F. (2017) New species and records of Perlidae (Plecoptera) from Espirito Santo State, Brazil. Zootaxa, 4273 (1), 141 - 150. https: // doi. org / 10.11646 / zootaxa. 4273.1.11"]}

Anacroneuria corae Bispo et al. 2014 Anacroneuria corae Bispo et al. 2014: 592. Material examined. BRA, TO, municipality of Palmas, Taquaru���� do Porto, Samba��ba, C��rrego Samba��ba, (- 10.3780388889, -48.1240111111), Pennsylvania trap, 1 female, 14���15.xi.2017, TKK, (CEUFT). BRA, TO, municipality of Palmas, Taquaru���� do Porto, Roncadeira, Ribeir��o Taquaru��uzinho, (-10.303639, -8.138861), Pennsylvania trap, 1 female, 18���19.xi.2017, MLSR, ASF, (CEUFT); 3 females and 1 male, 17���20.xi.2017, MLSR, ASF, (CEUFT). BRA, TO, municipality of Palmas, Taquaru���� do Porto, Vale do Vai Quem Quer, Ribeir��o S��o Jo��o (-10.394327, -48.132334), Pennsylvania trap, 2 females and 2 males, 13���14.xi.2017, TKK, (CEUFT), 2 females, 15���16.xi.2017, TKK, (CEUFT), 1 female 15���16.xi. 2 0 17, TKK, (INPA), 1 female, 15���16.xi.2017, TKK, (CLBA); Malaise trap, 1 female and 1 male, 13���16.xi.2017, TKK, (CEUFT), 1 female, 13���16.xi. 2 0 17, TKK, (INPA); light trap, 4 females and 3 males, 13���14.xi.2017, TKK, (CEUFT), 1 female and 2 males, 1 4���15.xi.2017, TKK, (CEUFT); light pan trap, 2 females and 5 males, 1 3���14.xi.2017, TKK, (CEUFT), 1 female and 1 male, 13��� 14.xi.2017, TKK, (CLBA), 4 males, 1 4���15.xi.2017, TKK, (CEUFT), 1 male, 14���15.xi.2017, TKK, (INPA). Measurements data. Female forewing: 8.4���12.6 mm, n= 21; male forewing: 7���9.1 mm, n= 20. Remarks. The specimens examined here present a variation in the size as compared to Bispo et al. (2014) (female forewing: 11.6���12.2 mm; male forewing: 8.1���8.3 mm). Additionally, teneral specimens collected (9 females and 8 males) do not present the characteristic distal less pigmented circular area in wings as described in Bispo et al. (2014). Egg oval (Fig. 4C), 0.23 x 0.33 mm. This species was previously recorded to Goi��s State, Brazil. In this paper, we provide a new record for Tocantins State, extending the distribution of the species., Published as part of Rippel, Mellis Layra Soares, Novaes, Marcos Carneiro & Krolow, Tiago K��tter, 2019, First records of the genus Anacroneuria (Plecoptera: Perlidae) from Tocantins State, Brazil and description of a new species, pp. 355-364 in Zootaxa 4560 (2) on page 359, DOI: 10.11646/zootaxa.4560.2.8, http://zenodo.org/record/2627721, {"references":["Bispo, P. C., Costa. L. S. M. & Novaes, M. C. (2014) Two new species and a new record of Anacroneuria (Plecoptera: Perlidae) from Central Brazil. Zootaxa, 3779 (5), 591 - 596. https: // doi. org / 10.11646 / zootaxa. 3779.5.9"]}

Anacroneuria marlieri Froehlich 2001 Anacroneuria marlieri Froehlich, in Ribeiro–Ferreira & Froehlich 2001: 191. Froehlich 2003: 130; Ribeiro & Rafael 2009: 7; Ribeiro & Gorayeb 2014:18; Ribeiro & Gorayeb 2016: 434. Material examined. BRA, TO, municipality of Palmas, Taquaruçú do Porto, Fazenda Buritizal, Córrego Buritizal, (-10.28667222, -48.05347222), light trap, 2 females, 27–28.iii.2018, TKK, (CEUFT), 1 female and 1 male, 28– 29.iii.2018, TKK, (CEUFT); light pan trap, 1 female and 1 male, 27–28.iii.2018, TKK, (CEUFT). BRA, TO, municipality of Palmas, Taquaruçú do Porto, Cachoeira do Evilson, Ribeirão Taquaruçú Grande, (-10.23193, - 48.123289), light trap, 1 male, 18–19.x.2017, MLSR, ASF, (CEUFT), 1 male, 18–19.x.2017, MLSF, ASF, (INPA), 1 male, 18–19.x.2017, MLSR, ASF, (CLBA), 2 females, 19–20.x.2017, MLSR, ASF, (CEUFT), 1 female, 19– 20.x.2017, MLSR, ASF, (INPA), 1 female, 19–20.x.2017, MLSR, ASF, (CLBA); light pan trap, 2 females and 1 male, 19–22.ix.2017, MLSR, ASF, (CEUFT). Measurements data. Female forewing: 10.9–14 mm, n= 10; male forewing: 7.4–10.4 mm, n= 9. Remarks. The specimens studied here differ in size from Ribeiro-Ferreira & Froehlich (2001) (male forewing length: 8.3 mm) and Froehlich (2003) (male forewing length: 8.2–9.3 mm; female forewing length: 11.6–12.0 mm). Furthermore, the pronotum of specimens presented by Ribeiro-Ferreira & Froehlich (2001) and Froehlich (2003) is characteristically whitish medially, which differs in part from the individuals studied here. Some specimens collected in Tocantins State have white band in the middle of pronotum and other have ochraceous stripes and rugosities in this portion. The penial armature is similar to that presented in Ribeiro & Rafael (2009), which is rounded apically. In Ribeiro-Ferreira & Froehich (2001), the apical portion of penial armature is tapered. The female of A. marlieri studied here has paramedial lobes of subgenital plate larger than lateral lobes, and median notch deeper than sublateral notch; which differs from Ribeiro & Rafael (2009). The egg is oval (Fig. 4D), 0.22 x 0.3 mm. This is the first record of this species for Tocantins State. This species has been recorded from Amazonas (Manaus, Novo Airão, Barcelos, Humaitá), Pará (Serra Norte), Maranhão (Imperatriz) and Mato Grosso (Chapada dos Guimarães) States (Ribeiro & Rafael 2009).

Anacroneuria minuta Klap��lek 1922 Figs. 3 A���3B. Anacroneuria minuta Klap��lek 1922: 89. Illies 1966: 319; Kimmins 1970: 345; Ribeiro-Ferreira & Froehlich 2001: 188; Froehlich 2002: 80; Ribeiro & Rafael 2009: 2; Ribeiro & Gorayeb 2014: 23; Ribeiro & Gorayeb 2016: 434. Material examined. BRA, TO, municipality of Palmas, Taquaru���� do Porto, Fazenda Buritizal, C��rrego Buritizal, (-10.28667222, -48.05347222), light trap, 1 male, 28���29.iii.2018, TKK, (CEUFT). BRA, TO, municipality of Palmas, Taquaru���� do Porto, Roncadeira, Ribeir��o Taquaru��uzinho, (-10.303639, -8.138861), Pennsylvania trap, 1 female and 1 male, 17���20.x.2017, MLSR, ASF, (CEUFT), 1 male, 18���19.x.2017, MLSR, ASF, (CEUFT), 1 male, 19���20.x.2017, MLSR, ASF, (CEUFT). BRA, TO, municipality of Palmas, Taquaru���� do Porto, Fazenda Encantada, Ribeir��o Taquaru���� Grande, (-10.244856, -48.123867), Pennsylvania trap, 1 female, 29.viii.2017, TKK, (CEUFT), 1 male, 30.viii.2017, TKK, (CEUFT). BRA, TO, municipality of Palmas, Taquaru���� do Porto, Cachoeira do Evilson, Ribeir��o Taquaru���� Grande, (-10.23193, -48.123289), light trap, 3 females, 29���30.viii.2017, TKK, (CEUFT), 3 females and 2 males, 18���19.x.2017, MLSR, ASF, (CEUFT), 6 females and 8 males, 19��� 20.x.2017, MLSR, ASF, (CEUFT); light pan trap, 2 females, 19���22.x.2017, TKK, (CEUFT). BRA, TO, municipality of Palmas, Taquaru���� do Porto, Vale do Vai Quem Quer, Ribeir��o S��o Jo��o (-10.394327, - 48.132334), Pennsylvania trap, 1 female and 2 males, 13���14.xi.2017, TKK, (CEUFT), 1 female and 1 male, 15��� 16.xi.2017, TKK, (CEUFT), 1 female, 15���16.xi.2017, TKK, (CLBA); Malaise trap, 1 female and 1 male, 13��� 16.xi.2017, TKK, (CEUFT); light trap, 1 female and 2 males, 1 3���14.xi.2017, TKK, (CEUFT), 1 female, 13��� 14.xi.2017, TKK, (INPA), 10 females and 11 males, 14���15.xi.2017, TKK, (CEUFT), 2 males, 14���15.xi.2017, TKK, (INPA), 2 males, 1 4���15.xi.2017, TKK, (CLBA); light pan trap, 1 female and 6 males, 13���14.xi.2017, TKK, (CEUFT), 3 males, 14���15.xi.2017, TKK, (CEUFT). Measurements data. Female forewing: 10���16.2 mm, n= 33; male forewing: 9���10.9 mm, n= 45. Remarks. The females studied here lack a distinct lateral stripe on the pronotum (Fig. 3A) as indicated in Ribeiro & Rafael (2009). Additionally, the head of the adult female of A. minuta in Ribeiro & Rafael (2009) is shown to be oval shaped, wider than long. In our specimens the head is longer than wide (Fig. 3A). The egg is elongate oval (Fig. 3B), 0.218 x 0.37. This species was previously recorded from Amazonas and Par�� states and new records of A. minuta are presented above for Tocantins State., Published as part of Rippel, Mellis Layra Soares, Novaes, Marcos Carneiro & Krolow, Tiago K��tter, 2019, First records of the genus Anacroneuria (Plecoptera: Perlidae) from Tocantins State, Brazil and description of a new species, pp. 355-364 in Zootaxa 4560 (2) on pages 360-361, DOI: 10.11646/zootaxa.4560.2.8, http://zenodo.org/record/2627721, {"references":["Ribeiro-Ferreira, A. C. & Froehlich, C. G. (2001) Anacroneuria Klapalek, 1909 from Amazonas States, North Brazil (Plecoptera, Perlidae, Anacroneuriinae). Aquatic Insects, 23, 187 - 192. https: // doi. org / 10.1076 / aqin. 23.3.187.4890","Froehlich, C. G. (2002) Anacroneuria mainly from southern Brazil and northeastern Argentina (Plecoptera: Perlidae). Proceedings of the Biological Society of Washington, 115 (1), 75 - 107.","Ribeiro, J. M. F. & Rafael, J. A. (2009) Redescription of the species of Anacroneuria Klapalek known from the Reserva Florestal Adolpho Ducke, Amazonas, Brazil, with a neotype designation, and a key to adult males, Amazonas, Brazil (Plecoptera, Perlidae). Zootaxa, 2004, 1 - 15."]}

Anacroneuria singularis Righi-Cavallaro & Lecci 2010 Anacroneuria singularis Righi-Cavallaro & Lecci 2010: 43. Novaes & Bispo 2014a: 435; Ribeiro et al. 2015: 296; Lecci & Righi-Cavallaro 2017: 2. Material examined. BRA, TO, municipality of Palmas, Taquaru���� do Porto, Roncadeira, Ribeir��o Taquaru��uzinho, (-10.303639, -8.138861), Pennsylvania trap, 1 male, 17���20.x.2017, MLSR, ASF, (CEUFT). BRA, TO, municipality of Palmas, Taquaru���� do Porto, Cachoeira do Evilson, Ribeir��o Taquaru���� Grande, (- 10.23193, -48.123289), light trap, 1 female and 1 male, 21���24.iii.2016, TKK, (CEUFT), 1 female, 21���24.iii.2016, TKK, (INPA), 1 female, 21���24.iii.2016, TKK, (CLBA); light pan trap, 1 male, 19���20.x.2017, MLSR, ASF, (CEUFT). BRA, TO, municipality of Palmas, Taquaru���� do Porto, Vale do Vai Quem Quer, Ribeir��o S��o Jo��o (- 10.394327, -48.132334), Pennsylvania trap, 1 male, 13���14.xi.2017, TKK, (CEUFT), 1 male, 15���16.xi.2017, TKK, (CEUFT), 1 male, 15���16.xi.2017, TKK, (INPA); light trap, 2 males, 14���15.xi.2017, TKK, (CEUFT); light pan trap, 1 male, 13���14.xi.2017, TKK, (CEUFT), 3 males, 14���15.xi.2017, TKK, (CEUFT), 1 male, 14���15.xi.2017, TKK, (CLBA). Measurements data. Female forewing: 16���17 mm, n= 3; male forewing: 9.85���11.9 mm, n= 13. Remarks. The penial armature of specimens studied here agree well with the description provided by Righi- Cavallaro & Lecci (2010). However, the head pattern varies from their illustrations, having a conspicuous M-line and central portion around M-line is dark yellow. Additionally, the general color is darker than mentioned in the original description (Righi-Cavallaro & Lecci 2010). Also, the pronotum has rugosities more apparent than in Righi-Cavallaro & Lecci (2010). Anacroneuria singularis has been recorded from Par��, Mato Grosso do Sul, S��o Paulo and Minas Gerais States. In this paper, we present the first record of this species for Tocantins State., Published as part of Rippel, Mellis Layra Soares, Novaes, Marcos Carneiro & Krolow, Tiago K��tter, 2019, First records of the genus Anacroneuria (Plecoptera: Perlidae) from Tocantins State, Brazil and description of a new species, pp. 355-364 in Zootaxa 4560 (2) on pages 361-362, DOI: 10.11646/zootaxa.4560.2.8, http://zenodo.org/record/2627721, {"references":["Righi-Cavallaro, K. O. & Lecci, L. S. (2010) Three new species of Anacroneuria (Plecoptera: Perlidae) from Centre-West and Southeast Brazil. Zootaxa, 2683, 35 - 44. https: // doi. org / 10.11646 / zootaxa. 2683.1.3","Bispo, P. C., Costa. L. S. M. & Novaes, M. C. (2014) Two new species and a new record of Anacroneuria (Plecoptera: Perlidae) from Central Brazil. Zootaxa, 3779 (5), 591 - 596. https: // doi. org / 10.11646 / zootaxa. 3779.5.9"]}

Tupiperla gracilis (Burmeister 1839) Semblis gracilis Burmeister, 1839, p. 876. Paragripopteryx gracilis — Illies, 1963 (in part), p. 179. Tupiperla gracilis — Froehlich, 1969 (in part), p. 29; Zwick, 1972, p. 1131; Froehlich, 1998, p. 20; Froehlich, 2010b, p. 138; Bispo and Lecci, 2011, p. 379; Avelino-Capistrano and Nessimian, 2013, p. 188; Novaes and Bispo, 2014, p. 439; Gonçalves et al., 2017b, p. 570. Material examined. BR, MG, SCNP, 1 female, Rio do Peixe (-20.2569, -46.4097), 17–19.x.2017, GFT, MZSP., Published as part of Novaes, Marcos Carneiro, Vilela, Diogo Silva, Lopez, Vinicius Marques & Ferreira, Rhainer Guillermo Nascimento, 2018, Certain species of Plecoptera from the headwater springs of National Integration River (São Francisco), Brazil, pp. 195-200 in Zootaxa 4429 (1) on page 197, DOI: 10.11646/zootaxa.4429.1.13, http://zenodo.org/record/1279732, {"references":["Burmeister, H. (1839) Handbuch der Entomologie, Plecoptera, Theod. Chr. Friedr. Endlin, Berlin, 2 (2), 863 - 881.","Illies, J. (1963) Revision der Sudamerikanischen Gripopterygidae (Plecoptera). Mitteilungen der Schweizerischen Entomologischen Gesellschaft, 36 (3), 145 - 248.","Froehlich, C. G. (1969) Studies on Brazilian Plecoptera 1. Some Gripopterygidae from the Biological Station at Paranapiacaba, State of Sao Paulo. Beitragezur Neotropischen Fauna, 6, 17 - 39.","Zwick, P. (1972) Die Plecopteren Pictets und Burmeisters, mit Angaben uber weitere Arten (Insecta). Revue Suisse de Zoologie, 78 (4), 1123 - 1194.","Froehlich, C. G. (1998) Seven new species of Tupiperla (Plecoptera: Gripopterygidae) from Brazil, with a revision of the genus. Studies on Neotropical Fauna and Environment, 33, 19 - 36. https: // doi. org / 10.1076 / snfe. 33.1.19.2170","Froehlich, C. G. (2010 b) Catalogue of Neotropical Plecoptera. Illiesia, 6 (12), 118 - 205.","Bispo, P. C. & Lecci, L. S. (2011) Gripopterygidae (Plecoptera) from Paranapiacaba mountains, southeastern Brazil. Annals of Limnology, 47, 373 - 385. https: // doi. org / 10.1051 / limn / 2011052","Avelino-Capistrano, F. & Nessimian, J. L. (2013) A new species and new records of Gripopterygidae (Plecoptera) from the Serra dos Orgaos, Rio de Janeiro State, Brazil. Zootaxa, 3683 (2), 185 - 191. https: // doi. org / 10.11646 / zootaxa. 3683.2.7","Novaes, M. C. & Bispo, P. C. (2014) Plecoptera from Minas Gerais State, southeastern Brazil. Zootaxa, 3856 (3), 433 - 442. https: // doi. org / 10.11646 / zootaxa. 3856.3.8","Goncalves, M. C., Novaes, M. C. & Salles, F. F. (2017 b) Studies on Gripopterygidae (Plecoptera) from Espirito Santo State, Brazil. Zootaxa, 4291 (3), 563 - 571. https: // doi. org / 10.11646 / zootaxa. 4291.3.8"]}

Anacroneuria sp. 1 Material examined. BR, MG, SCNP, 1 nymph, main spring of São Francisco River (20.2436, -46.4464), 19.x.2017, GFT, MZSP. Remarks. It is not presently possible to identify specifically the nymph of this taxon collected from this spring. The general habitus including the coloration appears to be different from other nymphs studied from this area. Associated male adults will be necessary for positive identification.

Anacroneuria saofrancisco n. sp. Figures 2–6. Material examined. Holotype male: BRAZIL (BR), Minas Gerais State (MG), Serra da Canastra National Park (SCNP), Rio do Peixe (-20.2569, -46.4097), 17–19.x.2017, GFT, MZSP. Paratypes: BR, MG, SCNP, 2 males and 3 females, Rio do Peixe (20.2569, -46.4097), 17–19.x.2017, GFT, MZSP. Additional material: BR, MG, SCNP, 2 nymphs, Rio do Peixe (-20.2569, -46.4097), 17–19.x.2017, GFT, MZSP. Description. General color brown. Head light brown with central part yellowish; clypeal area setose; M-line incomplete, slightly visible and with diffuse limits; (Fig. 2); lappets brown; gena and parietalia light brown; postfrontal line U-shaped; antenna and palps brow. Pronotum dark brown, with whitish bands medially and laterally (Fig. 2). Legs, femora light brown, tibia and tarsi darker. Wing membrane and veins brown. Cerci brown. Male. Forewing length 11.1–11.7 mm. Hammer a truncate cone (Fig. 3). Penial armature with a pair of small ventral, membranous lobes (Figs. 4–6); in dorsal view, 3-lobed apically with lateral lobes (shoulders) well sclerotized (Fig. 4); ventral apical part (Fig. 5) a thin flap-shaped sclerotized structure (ventral membranous lobes are attached to this structure); hooks relatively large, regularly curved and with apex pointed (Fig. 5); dorsal keel absent. Female. Forewing length 14.7–15.5 mm. Subgenital plate light brown and 4-lobed (Fig. 7). Median notch relatively deep, mesal lobes wider than lateral ones. Lateral lobes slightly shorter than medial ones. Egg elongate oval, ca. 0.22 x 0.45 mm (Fig. 8). Nymph. General color dark brown. Head dark brown with lighter areas near ocelli, compound eyes and parietallia (Fig. 9); M-line slightly visible and with diffuse limits; eyes and ocelli black. Pronotum dark brown with median line and scattered areas light brown (Fig. 9). Mandibles, maxillae, and labium as in Figs. 10–12, respectively. Forefemur with lateral surface covered by sparse bristles (Fig. 13); dorsally and ventrally with higher density of thick bristles; dorsally, with strong fringe of hairs. Tibia ventrally with sparse thick bristles, and dorsally with a row of thick bristles and a well-developed band of hairs. Cerci dark brown with thick bristles; segments differ in shape and size from base to apex (Fig. 14). Thoracic gills: ASC 1, PSC1, AT2, AT3 and PT3 (sensu Shepard & Stewart 1983; Stewart & Stark 2002). Measurements (n=5): head width 2.9–3.1 mm; head length 1.7– 1.9 mm; antennae length 5.5–5.8 mm; pronotum width 3.0– 3.4 mm; pronotum length 1.8–2.5 mm; cercal length 6.9–7.1 mm; total length (without cerci) 10.9–11.5 mm. Remarks. The penial armature of A. saofrancisco resembles A. tabatae Froehlich 2010a. However, the apical penial structure of A. saofrancisco is wider than A. tabatae, as shown in ventral, dorsal, and lateral views (Figs. 4– 6). Additionally, A. tabatae has only a single ventral membranous lobe, whereas A. saofrancisco has two. The head and pronotal color pattern of the nymphs of A. saofrancisco resembles that of A. flintorum Froehlich 2002 (Almeida et al., 2018). However, the nymph of A. saofrancisco is lighter than A. flintorum. Furthermore, the M-line in A. flintorum is well defined but indistinct in A. saofrancisco. Etymology. This name refers to the São Francisco River where the specimens were collected.

Novaes, Marcos Carneiro, Vilela, Diogo Silva, Lopez, Vinicius Marques, Ferreira, Rhainer Guillermo Nascimento (2018): Certain species of Plecoptera from the headwater springs of National Integration River (São Francisco), Brazil. Zootaxa 4429 (1): 195-200, DOI: https://doi.org/10.11646/zootaxa.4429.1.13

Anacroneuria flintorum Froehlich, 2002 Anacroneuria flintorum Froehlich, 2002: 93; Bispo & Froehlich, 2004: 99; De Ribeiro & Froehlich, 2007: 55; Froehlich, 2010: 56; Baldin et al., 2013: 394; Gon��alves et al., 2017: 146. Material examined. BR, SP: Iporanga, Gruta da Tapagem, 15.xi.1991, 1 male; PEI, Rio do Carmo, 19.ii.1992, 1 male; PEI, Rio do Carmo, 16.ii.1993, 2 males; PEI, Rio Po��os Altos, 10.xi.1993, CGF & HP col., 2 males; PETAR, Rio Betari, Bairro da Serra, 20.viii.1996, light trap, ASM col., 1 male; PEI, C��rrego Lajeado, 24.ii.1999, 2 males; PEI, C��rrego Lajeado, 17.ix.1999, 1 male; PEI, Ribeir��o ��gua Comprida, 27.x.1999, 3 males; PEI, Rio do Carmo, 28.x.1999, 1 male; PEI, Ribeir��o ��gua Comprida, 23.xi.1999, 7 males; PEI, Ribeir��o ��gua Comprida, 06.i.2000, 8 males; PEI, Rio Po��os Altos, 01.ii.2000, 2 males; PEI, Rio do Carmo, 25.iii.2000, 1 male; PEI, Ribeir��o ��gua Comprida, 24��17���38���S, 48��25���04���W, 30.x.2002, ASM col., 1 male; PECB, Rio Taquaral, 09.ix.2006, 1 male; PEI, C��rrego Roda D�����gua, 13.xii.2014, light trap, PCB col., 1 female; PEI, Rio do Carmo, bridge, 24��18���15���S, 48��24���31���W, 14.xii.2014, light trap, PCB col., 1 male and 1 female; PEI, C��rrego do Mirante, 15.xii.2014, light trap, PCB col., 1 female; PEI, Rio do Carmo, bridge, 24��18���15���S, 48��24���31���W, 08.ii.2017, light trap, LHA et al. col., 1 male; PEI, Ribeir��o Bocaina, bridge, 24��16���13���S, 48��27���09���W, 12.ii.2017, light trap, LHA et al. col., 1 male; PETAR N��cleo Santana, Riacho Furnas, 24��32���03���S, 48��42���02���W, 14.ii.2017, light trap, LHA et al. col., 2 males; PETAR N��cleo Santana, Rio Roncador, 24��32���00���S, 48��42���06���W, 15.ii.2017, light pan trap, LHA et al. col., 1 male; PECB, Bra��o do Rio Taquaral, 24��03���31���S, 47��59���41���W, 05.ii.2017, D-net, LHA et al. col., 1 nymph; PECB, Ribeir��o de Pedras, 24��03���40���S, 47��59���51���W, 06.ii.2017, D-net, LHA et al. col., 1 nymph; PECJ, C��rrego Campo do Meio 22��41���28���S, 45��28���26���W, 28.i.2009, light trap, 2 males. BR, ES: Capara��, Rio Santa Marta, 18.ii.2016, FFST col., 3 females and 7 nymph; Capara��, Pedra Menina, Base, 14-15.ii.2016, FFST col., 2 females; Capara��, Pedra Menina, Base, 15-16.ii.2016, FFST col., 6 females and 1 male. BR, MG: Araponga, Serra do Brigadeiro, Vale das Luas, 14.i.2014, FFST col. 1 nymph. BR, SC: Chapad��o, 28#12���09���S, 49#23���14���W, 15.ii.2013, light trap, L.C. Pinho, A.P. Amaral, M.C. Novaes col., 1 male; Orleans, Rio da Serra, 28��12���43���S, 49#27���28���W, 16.ii.2013, light trap, L.C. Pinho, A.P. Amaral, M.C. Novaes col., 1 male. Description and association of nymph. Molecular association. We associated adults with nymphs of A. flintorum (including teneral adults) from the states of Esp��rito Santo and S��o Paulo. The COI divergences ranged from 0���0.9% for specimens from the S��o Paulo State and from 0���0.7% from Esp��rito Santo State. Intraspecific divergences ranged from 0���3.9%, probably due the geographic variation. For all species studied, interspecific divergences ranged from 12.4%, between A. flintorum (PLE481a) and A. sp. 2 (PPI 15) to 20.6% between A. flintorum (PLE479a) and A. debilis (Pictet, 1841) (PLE480b). A dendrogram and the matrix with pair divergence between specimens based on COI sequences are presented in Fig. 1 and Table 2. Description of nymph. General color dark brown in dorsal view and light yellow in ventral view. Head dark brown with light yellow areas near post-frontal suture, occiput and frontoclypeal region; M-line complete, with well-defined limits; eyes and ocelli black; ocelli with a translucent layer (Fig. 2A); clypeus dark brown; labrum light yellow; maxillae light yellow with lacinia light brown and galea brown; mandible black with median region dark brown with f5 pointed teeth on distal margin, decreasing in size toward base, but teeth 3 and 4 similar in size (Figs. 3A, 3B and 3C). Pronotum dark brown with thin median line and scattered rugosities light yellow (Fig. 2A). Wing pad light with contour partially dark brown (Fig. 2C). Leg brown, forefemur with a central pale line without setae and with lateral surface covered by sparse bristles, in dorsal view; forefemur anteriorly and posteriorly with higher density of thick bristles, and posteriorly with fringe of hairs; tibia anteriorly with sparse thick bristles, and posteriorly with a row of thick bristles and a well-developed band of hairs (Figure 3D). Abdomen dark brown, almost black, with a drop-shaped light spot in the last segment (Fig. 2B). Cerci brown with thick bristles; segments differ in shape and size from base to apex (Fig. 3E). Thoracic gills (Fig. 2D): ASC 1, AT2, AT3 and PT3. Measurements (n=4): head width 3-5 mm; head length 2-3 mm; antennae length 7-8 mm; pronotum width 3-3.5 mm; pronotum length 1.5- 2 mm; cercal length 9-12 mm; total length 10-15mm. Adult morphological variation. Examination of specimens from different regions of Atlantic Forest (states of Esp��rito Santo, Minas Gerais, Santa Catarina, and S��o Paulo) indicated morphological variation. Forewing length of male adults of A. flintorum range from 10 to 15.2 mm (specimens studied herein and Bispo & Froehlich, 2004; Froehlich, 2010, and Baldin et al., 2013). The dorsal color pattern of head of adult of A. flintorum can be variable, even within the same population (Bispo & Froehlich, 2004). In addition, it is possible to observe that the dark brown color of recently collected specimens can become ochraceous when preserved in 80% and 100% ethanol for an extended period of time (Fig. 4). Therefore, it is difficult to identify the species by color pattern alone. For identification, it is essential to study the male penial armature, which in A. flintorum is elongated, tapering progressively from base to the apex and has hooks well separated from distal vesicles (Froehlich, 2002; Bispo & Froehlich, 2004). However, the keel of penial armature may diverge in specimens from the same population and in different populations. In dorsal view, the keel varies from rounded (male specimens from Rio de Janeiro State), pointed (male specimens from PECJ), to convergent or parallel lines (male specimens from PEI). The specimens collected farther north of the species distribution (e. g. ES, RJ and North of SP) have penial armature more robust than specimens from further south (e.g. South of SP and SC). Teneral adult specimens. Teneral specimens of A. flintorum studied are generally brown in color with pale areas along the body; head and pronotum brown with a pale outline. In these specimens, the pronotum does not have lateral folds at the edges, wings are totally pale and the thoracic segments present remnants of gills (Figure 5D, 5E and 5F). A similar pattern to A. flintorum is also present in A. debilis (Almeida & Duarte, 2017) suggesting that several species may be similar when teneral. Therefore, species described as having pale areas on the head and pronotum and milky wings (e.g. A. petersi Froehlich, 2002) may have been based on teneral specimens, requiring caution for use in identifications. Remarks. The nymph of A. flintorum has a different pattern than the other known Anacroneuria nymphs described from Brazil (Righi-Cavallaro & Lecci, 2010; Avelino-Capistrano et al., 2011a; Novaes et al., 2012; Righi-Cavallaro & Froehlich, 2013; Ribeiro & Gorayeb, 2014; Gon��alves et al., 2017). The color pattern of the head and the drop-shaped light spot in the last abdominal segment are apparently characteristic for this species. In addition to description of the nymph, we present new species records from the states of Minas Gerais and Santa Catarina. Anacroneuria flintorum has been collected from streams from protected areas of the Atlantic Forest (Fig. 6). In general, these streams are pristine and have fast currents and rocky substrates, and undisturbed channels and banks. This suggests that A. flintorum may be a sensitive taxon to environmental disturbances., Published as part of Almeida, Lucas Henrique De, Gon��alves, Ma��sa De Carvalho, Novaes, Marcos Carneiro, Paresqui, Rayner Constantino & Bispo, Pit��goras Da Concei����o, 2018, Anacroneuria flintorum Froehlich 2002 (Plecoptera: Perlidae): Notes, distribution, and life stages association using molecular tools, pp. 409-420 in Zootaxa 4370 (4) on pages 411-415, DOI: 10.11646/zootaxa.4370.4.6, http://zenodo.org/record/1146515, {"references":["Froehlich, C. G. (2002) Anacroneuria mainly from southern Brazil and northeastern Argentina (Plecoptera: Perlidae). Proceedings of the Biological Society of Washington, 115, 75 - 107.","Bispo, P. C. & Froehlich, C. G. (2004) Perlidae (Plecoptera) from Intervales State Park, Sao Paulo State, Southeastern Brazil, with descriptions of new species. Aquatic Insects, 26 (2), 97 - 113. https: // doi. org / 10.1080 / 01650420412331325837","De Ribeiro, V. R. & Froehlich, C. G. (2007) Two new species of Anacroneuria (Plecoptera: Perlidae) from southern Brazil. Zootaxa, 1624, 53 - 57.","Froehlich, C. G. (2010) Anacroneuria (Plecoptera, Perlidae) from the Mantiqueira Mountains, Sao Paulo State, Brazil. Zootaxa, 2365, 55 - 68.","Baldin, C., Bispo, P. C. & Novaes, M. C. (2013) New species and records of Anacroneuria (Plecoptera: Perlidae) from Rio de Janeiro State, Brazil. Zootaxa, 3694 (4), 391 - 397. https: // doi. org / 10.11646 / zootaxa. 3694.4.7","Goncalves M. C., Novaes, M. C. & Salles, F. F. (2017) New species and records of Perlidae (Plecoptera) from Espirito Santo State, Brazil. Zootaxa, 4273 (1), 141 - 150. https: // doi. org / 10.11646 / zootaxa. 4273.1.11","Pictet, F. - J. (1841) Histoire Naturelle Generale et Particuliere des Insectes Nevropteres. Famille des Perlides. J. Kessmann, Geneve, U. S. A. https: // doi. org / 10.5962 / bhl. title. 124172","Almeida, L. H. & Duarte, T. (2017) A new species and records of Anacroneuria (Plecoptera: Perlidae) from the Ecological Station, Wenceslau Guimaraes, State of Bahia, Brazil. Zootaxa, 4247 (4), 480 - 486. https: // doi. org / 10.11646 / zootaxa. 4247.4.9","Righi-Cavallaro, K. O. & Lecci, L. S. (2010) Three new species of Anacroneuria (Plecoptera: Perlidae) from Centre-West and Southeast Brazil. Zootaxa, 2683, 35 - 44.","Avelino-Capistrano, F., Barbosa, L. S. & Almeida, G. L. (2011 a) The nymph of Anacroneuria debilis (Pictet, 1841) (Plecoptera: Perlidae). Zootaxa, 2882, 59 - 63.","Novaes, M. C., Bispo, P. C. & Higuti, J. (2012) A description of the nymph of Anacroneuria ofaye Froehlich (Plecoptera: Perlidae) and a apparatus for rearing Neotropical stonefly species. Zootaxa, 3547, 71 - 77.","Righi-Cavallaro, K. O. & Froehlich, C. G. (2013) The Nymph of of Anacroneuria payagua Froehlich (Plecoptera: Perlidae) from Brazil. Zootaxa, 3635 (5), 579 - 582. https: // doi. org / 10.11646 / zootaxa. 3635.5.7","Ribeiro, J. M. F. & Gorayeb, I. S. (2014) Description of immatures and association with adults of three species of Anacroneuria Klapalek (Plecoptera: Perlidae) of the Brazilian Amazon. Zootaxa, 3881 (1), 17 - 32. https: // doi. org / 10.11646 / zootaxa. 3881.1.2"]}

Anacroneuria piti sp. n. Figs. 2A–2 D (male) and Figs. 3A–3 F (nymph). Material examined. Holotype male (CZNC): Brazil (BRA), Espírito Santo State (ES), Alegre, Rio Norte, reared, nymph collected 07.viii.2004, emerged 08.viii.2004. Description. General color yellowish. Head yellowish with M-line not distinct, parts of clypeal area yellowish and frons light brown (Fig. 2A); lappets brown; clypeal area setose; post frontal line not distinct; scape and pedicel light brown; flagellomeres light brown; palpi light brown. Pronotum light brown with lighter rugosities (Fig. 2A). Legs yellowish to light brown, tibia and tarsi darker. Wing membrane light brown with veins darker. Cerci light brown. Male: Forewing length 11mm (n=1). Penial armature (Figs. 2 B–2D), in ventral view, gradually tapering from medially to apex and terminating in an indistinct small aperture (Fig. 2 C), with a pair of small distal membranous vesicles (Figs. 2 C–2D); in lateral view, pronounced keel apparent (Fig. 2 D), distinct in dorsal view appearing as two parallels bars (Fig. 2 B); hooks relatively short and flattened, little curved and with acute apices (Fig. 2 C). Nymph: General color brownish. Head brown with lighter areas near post-frontal suture and in frontoclypeal region (Fig. 3A); M-line complete, distinct; eyes black and ocelli black with a darker stain medially. Pronotum brownish with median line and sparse areas yellowish (Fig. 3A). Mandibles, maxillae, and labium as in Figs. 3 B, 3C, 3D. Forefemur with lateral surface covered by scattered bristles (Fig. 3 E); dorsally and ventrally with higher density of thick bristles; dorsally with translucent fringe of hairs. Tibia ventrally with thin and sparse translucent bristles, and dorsally with a row of thick bristles in the border and a well-developed band of hairs. Cerci brown with thick bristles; segments differ in shape and size from base to apex (Fig. 3 E). Thoracic gills: ASC 1, PSC1, AT2, AT3 and PT3 (sensu Shepard & Stewart 1983; Stewart & Stark 2002). Measurements (n=7): head width 2.9– 3.3 mm; head length 1.9–2.6 mm; antennae length 4.0–5.0mm; pronotum width 2.5–3 mm; pronotum length 1.5–2 mm; cercal length 6.5–7.5 mm; total length 7.9–9 mm. Remarks. The color of the adult is similar to many species of the genus. The penial armature of A. pitii in ventral view (Fig. 2 C) and lateral view (Fig. 2 D) is most similar to A. itatiaiensis Baldin et. al 2013 also known from southeastern Brazil, but differs in the size of membranous vesicles and apex. In A. pitii, the vesicles are smaller and are more medially than in A. itatiaiensis. The penial armature (Figs. 2 B–2D) of A. pitii is more robust basally than A. itatiaiensis. In lateral view (Fig. 2 D), the apex of A. pitii is thinner than A. itatiaiensis; the hooks are more robust in A. piti. Furthermore the color of A. pitii is lighter than A. itatiaiensis. Etymology. The patronym honors Pitágoras da Conceição Bispo, a Brazilian Plecoptera specialist.

Anacroneuria atrifrons Klap��lek 1922 Material examined. BRA, ES, Municipality of Alegre, Cachoeira do Firmino, S20��37'11.4" W41��37'35.4", 649 m, light trap, 04���05.iv.2016, 1 male, FFST, CZNC. Remarks. This is a new record for Esp��rito Santo State, Brazil., Published as part of Gon��alves, Ma��sa De Carvalho, Novaes, Marcos Carneiro & Salles, Frederico Falc��o, 2017, New species and records of Perlidae (Plecoptera) from Esp��rito Santo State, Brazil, pp. 141-150 in Zootaxa 4273 (1) on page 146, DOI: 10.11646/zootaxa.4273.1.11, http://zenodo.org/record/801867, {"references":["Klapalek, F. (1922) Plecopteres nouveaux. Quatrieme partie. Annales de la Societe Entomologique de Belgique, 62, 89 - 95."]}

Anacroneuria amargosa Righi-Cavallaro & Froehlich, 2013 Material examined. BRA, ES, Municipality of Linhares, Fazenda Amparo, S19°26'50.8" W39°56'29.2", 0 m, light trap, 23–24.ix.2014, 1 male, FFST, CZNC. BRA, ES, Municipality of Linhares, Fazenda Rio Claro, S19°32'18.2" W39°52'59.3", 0 m, light trap, 23–24.ix.2014, 1 male, FFST, CZNC. BRA, ES, Municipality of Nova Venécia, Santa Rita do Pip Nuck, Rio Cricaré, S18°39'51.4" W40°30'44.9", 74 m, light trap, 18–19.ii.2013, 1 male, FFST, CZNC. Remarks. This is a new record for Espírito Santo State, Brazil.

Gripopteryx cancellata (Pictet 1841) Material examined: BRA, ES, PNC, Municipality of Alto Capara��, border between Minas Gerais and Esp��rito Santo States, Rio S��o Domingos, Cachoeira da Farofa, S20��28'19.5" W41��49'41.7", 1972 m, D-net, 15.ii.2016, 2 nymphs, FFST, CZNC. BRA, ES, PNC, Municipality of Alto Capara��, border between Minas Gerais and Esp��rito Santo States, Cachoeira do Aur��lio, S 20�� 28' 57.5'', W 41�� 50' 14.5'', 1800 m, D-net and light trap, 22.iv.2008, 1 nymph and 1 female, FFST, CZNC. BRA, ES, PNC, Municipality of Alto Capara��, border between Minas Gerais and Esp��rito Santo States, Vale Encantado, S 20��24'38.7'' W 41�� 50' 3.6'', 1976 m, D-net and light trap, 30.v.2011, 1 nymph, 1 male and 1 female, FFST, CZNC. BRA, ES, PNC, Municipality of Alto Jequitib��, border between Minas Gerais and Esp��rito Santo States, Cachoeira Bonita, S20��24'27.7" W41��50'24.1", 1329 m, D-net and light trap, 12.x.2012, D-net, 1 nymph, FFST, CZNC. BRA, ES, PNC, Municipality of Espera Feliz, border between Minas Gerais and Esp��rito Santo States, Pedra Menina, S 20��37'30.3" W 41��49'27.1", 884 m, D-net, 16.ii.2016, 9 nymphs, FFST, CZNC. BRA, ES, PNC, Municipality of Ibitirama, Santa Marta, Rio Santa Marta, S20��29'51.3" W41��43'45.9", 935 m, D-net, 31.v.2011, 1 nymph, FFST, CZNC. Remarks. This is a new record for Esp��rito Santo State, BRA., Published as part of Gon��alves, Ma��sa De Carvalho, Novaes, Marcos Carneiro & Salles, Frederico Falc��o, 2017, Studies on Gripopterygidae (Plecoptera) from Esp��rito Santo State, Brazil, pp. 563-571 in Zootaxa 4291 (3) on pages 564-566, DOI: 10.11646/zootaxa.4291.3.8, http://zenodo.org/record/827728, {"references":["Pictet, F. (1841) Histoire Naturelle Generale et Particuliere des Insectes Nevropteres. Famille des Perlides, 1 ere Partie. Kessmann, Geneve, 423 pp."]}