50 results on '"Noto AM"'
Search Results
2. METODI FISICO E BIOLOGICO DI IDENTIFICAZIONE DI ALIMENTI IRRADIATI CONTENENTI CELLULOSA ATTRAVERSO L'USO DELLA DNA COMET ASSAY E DELLE SPETTROSCOPIA ESR
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D'OCA, Maria Cristina, BARTOLOTTA, Antonio, PARLATO, Aldo, Garofalo, C, Di Noto, AM, Cardamone, C., D'Oca, MC, Bartolotta, A, Garofalo, C, Parlato, A, Di Noto, AM, and Cardamone, C
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COMET ASSAY ,ALIMENTI IRRADIATI ,SPETTROSCOPIA ESR ,Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin) - Published
- 2013
3. Fast Extraction Procedure of 2- DodecylCycloButanone for the identification of irradiated pork, employing Gaschromatographic/Mass Spectrometric analysis
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D'OCA, Maria Cristina, Bartolotta, A, Di Noto, AM, Cardamone, C, Ferrantelli, V, Macaluso, A, Spagnolo, D, Giaccone, V, Boniglia, C, Giammarioli, S, Benetti, C, Pizzoccheri, V, Sangiorgi, E., D'Oca, MC, Bartolotta, A, Di Noto, AM, Cardamone, C, Ferrantelli, V, Macaluso, A, Spagnolo, D, Giaccone, V, Boniglia, C, Giammarioli, S, Benetti, C, Pizzoccheri, V, and Sangiorgi, E
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GAS CROMATROGRAFIA E SPETTROMETRIA DI MASSA ,ALIMENTI IRRADIATI ,Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin) ,METODI CHIMICI DI IDENTIFICAZIONE - Published
- 2012
4. Il latte e i suoi derivati: un alimento senza limiti di età
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Scatassa, ML, Giangrosso, G, Costantino, VD, Currò, V, Di Noto, AM, Migliazzo, A, Cusumano, F, Villari, S, TODARO, Massimo, Scatassa, ML, Giangrosso, G, Costantino, VD, Currò, V, Di Noto, AM, Migliazzo, A, Cusumano, F, Villari, S, and Todaro, M
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latte, derivati del latte ,Settore AGR/19 - Zootecnica Speciale - Published
- 2009
5. VALUTAZIONE DELL'ESPOSIZIONE A FITOFARMACI IN POPOLAZIONE GENERALE DI UN COMUNE SICILIANO: DATI PRELIMINARI
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CALAMUSA, Giuseppe, ROMANO, Nino, AMODIO, Emanuele, MASSENTI, Maria Fatima, FIRENZE, Alberto, DI PASQUALE, M, TERMINI, S, COSTANTINO, Claudio, GELSOMINO, V, GIOE', S, MORICI, M, LORIA, A, PALMERI, A, SAFINA, P, DI NOTO, AM, CARDAMONE, C, D'OCA, T, VITALE, Francesco, CALAMUSA, G, ROMANO, N, AMODIO, E, MASSENTI, MF, FIRENZE, A, DI PASQUALE, M, TERMINI, S, COSTANTINO, C, GELSOMINO, V, GIOE', S, MORICI, M, LORIA, A, PALMERI, A, SAFINA, P, DI NOTO, AM, CARDAMONE, C, D'OCA, T, and VITALE, F
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fitofarmaci - Published
- 2009
6. Vastedda della valle del Belice cheese: experimental contamination with Salmonella and Listeria spp
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Scatassa, ML, Di Noto, AM, Cardamone, C, Sciortino, S, Caracappa, S., TODARO, Massimo, Scatassa, ML, Di Noto, AM, Cardamone, C, Sciortino, S, Todaro, M, and Caracappa, S
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Settore AGR/19 - Zootecnica Speciale ,Vastedda cheese, Salmonella, Listeria - Published
- 2009
7. Vastedda della valle del Belìce: processo produttivo e contaminazione sperimentale con Salmonella spp
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Scatassa, ML, Di Noto, AM, Cardamone, C, Sciortino, S, Caracappa, S., TODARO, Massimo, Scatassa, ML, Di Noto, AM, Cardamone, C, Sciortino, S, Todaro, M, and Caracappa, S
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Settore AGR/19 - Zootecnica Speciale ,Vastedda della valle del Belìce, sicurezza alimentare, Salmonella spp - Published
- 2009
8. A fast extraction procedure of 2-dodecylcyclobutanone for the identification of irradiated pork, employing gas chromatographic/mass spectrometric analysis
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D'OCA, Maria Cristina, BARTOLOTTA, Antonio, CAMMILLERI, C, GIUFFRIDA, S, DARA, S, DI NOTO, AM, D'OCA, MC, BARTOLOTTA, A, CAMMILLERI, C, GIUFFRIDA, S, DARA, S, and DI NOTO, AM
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gas chromatography, irradiated food, extraction procedure - Published
- 2008
9. Determinazione tramite GC/MS di etilentiourea (ETU) in urine di lavoratori di una azienda vitivinicola siciliana esposti a mancozeb
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DI NOTO, AM, D'OCA, Maria Cristina, CARDAMONE, C, RANDISI, B, DARA, S, CARACAPPA, S, VERSO, Maria Gabriella, SCHILLACI, Salvatore, PICCIOTTO, Diego, DI NOTO, AM, D'OCA, MC, CARDAMONE, C, RANDISI, B, DARA, S, CARACAPPA, S, VERSO, MG, SCHILLACI, S, and PICCIOTTO, D
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ETU, mancozeb, ditiocarbammati, operatori agricoli - Published
- 2008
10. RESIDUI DI FITOSANITARI IN FLUIDI BIOLOGICI UMANI E VALUTAZIONE DELL'ESPOSIZIONE IN POPOLAZIONE GENERALE DI UN COMUNE SICILIANO: PROGETTAZIONE DI UNO STUDIO
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CALAMUSA, Giuseppe, ROMANO, Nino, FIRENZE, Alberto, DI PASQUALE, Maria, AMODIO, Emanuele, DI NOTO AM, CARDAMONE C, D'OCA T, VITALE, Francesco, CALAMUSA G, ROMANO N, FIRENZE A, DI PASQUALE M, AMODIO E, DI NOTO AM, CARDAMONE C, D'OCA T, and VITALE F
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RESIDUI FITOSANITARI - Published
- 2008
11. Vastedda della valle del Belìce cheese: production and microbiological investigation
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Scatassa, ML, Di Noto, AM, Caracappa, S., TODARO, Massimo, Scatassa, ML, Di Noto, AM, Todaro, M, and Caracappa, S
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Settore AGR/19 - Zootecnica Speciale ,vastedda cheese, microbiological investigation - Published
- 2007
12. INDAGINE MICROBIOLOGICA SU MAIALE IRRADIATO A SCOPO CONSERVATIVO E STIMA DELLA DOSE TRAMITE GC/MS
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GIUFFRIDA SA, CARDAMONE C, CAMMILLERI MC, D'OCA MC, FERRUGIA M, PARLATO, Aldo, DI NOTO AM, NIFOSI' D., BARTOLOTTA, Antonio, GIUFFRIDA SA, CARDAMONE C, CAMMILLERI MC, D'OCA MC, BARTOLOTTA A, FERRUGIA M, PARLATO A, DI NOTO AM, and NIFOSI' D
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- 2007
13. Isolamento in alimenti di Enterococcus spp resistenti ai glicopeptidi (GRE)
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DI NOTO AM, CARDAMONE C, COSTA A, DI CARLO, Paola, MAMMINA, Caterina, DI NOTO AM, CARDAMONE C, COSTA A, DI CARLO P, and MAMMINA C
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- 2006
14. Molecular epidemiology of Salmonella enterica serotype Infantis from human and non human sources in southern Italy, 2000-2005
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MAMMINA, Caterina, ALEO, Aurora, GIAMMANCO, Giovanni, DI NOTO AM, COSTA A, CARACAPPA S., MAMMINA C, DI NOTO AM, ALEO A, COSTA A, GIAMMANCO GM, and CARACAPPA S
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- 2006
15. GAS CHROMATOGRAPHIC/MASS SPECTROMETRIC AND MICROBIOLOGICAL ANALYSISES ON IRRADIATED CHICKEN
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PARLATO, Aldo, GIUFFRIDA SA, AVELLONE, Giuseppe, DI NOTO AM, CARACAPPA S., CALDERARO, Elio Giuseppe, BARTOLOTTA, Antonio, D'OCA, Maria Cristina, BRAI, Maria, TRANCHINA, Luigi, PARLATO A, CALDERARO E, BARTOLOTTA A, D'OCA MC, GIUFFRIDA SA, BRAI M, TRANCHINA L, AVELLONE G, DI NOTO AM, and CARACAPPA S
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- 2006
16. IDENTIFICAZIONE CON GC/MS E INDAGINE MICROBIOLOGICA DI POLLO IRRADIATO
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BARTOLOTTA A, D'OCA MC, GIUFFRIDA SA, BRAI M, TRANCHINA, Luigi, PARLATO, Aldo, DI NOTO AM, COSTA A, CARACAPPA S., AVELLONE, Giuseppe, BARTOLOTTA A, D'OCA MC, GIUFFRIDA SA, BRAI M, TRANCHINA L, AVELLONE G, PARLATO A, DI NOTO AM, COSTA A, and CARACAPPA S
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Settore CHIM/10 - Chimica Degli Alimenti ,pollo irradiato, GC-MS - Published
- 2005
17. Sierotipi di Salmonella identificati da fonte umana, alimentare ed animale in Sicilia, 2003-2005
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MAMMINA, Caterina, ALEO, Aurora, GUIDA, Ivana, DI NOTO AM, COSTA A, CARDAMONE C, FERRANTELLI V, CARACAPPA S., MAMMINA C, DI NOTO AM, ALEO A, GUIDA I, COSTA A, CARDAMONE C, FERRANTELLI V, and CARACAPPA S
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- 2005
18. Indagine sulla presenza di Escherichia coli O157 in prodotti carnei
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DI NOTO AM, COSTA A, CARDAMONE C, RUSSO EM, OLIVERI G, MAMMINA, Caterina, DI NOTO AM, COSTA A, CARDAMONE C, RUSSO EM, OLIVERI G, and MAMMINA C
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- 2005
19. The gas chromatography/mass spectrometry can be used for dose estimation in irradiated pork
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Maria Cristina D'Oca, Aldo Parlato, Antonio Bartolotta, M.C. Cammilleri, Santo Caracappa, S. Giuffrida, A Di Noto, D'Oca, MC, Di Noto, AM, Bartolotta, A, Camilleri, MC, Giuffrida, SA, Parlato, A, Ferrugia, M, D'OCA, MC, BARTOLOTTA, A, CAMMILLERI, MC, GIUFFRIDA, SA, PARLATO, A, DI NOTO, AM, and CARACAPPA, S
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education.field_of_study ,Radiation ,Chromatography ,Chemistry ,Population ,Food spoilage ,Radappertization ,gas chromatography/mass spectrometry,irradiated pork ,Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin) ,Food irradiation, gas chromatography, microbial contamination, radiation treatment, dose assessment ,media_common.cataloged_instance ,Food irradiation ,Irradiation ,Gas chromatography ,Gas chromatography–mass spectrometry ,European union ,education ,media_common - Abstract
Food safety can be improved using ionizing radiation to reduce food spoilage and to extend its shelf life. The gas chromatography/mass spectrometry (GC/MS) has been validated by the European Community as a powerful method to identify irradiated food containing fat. The preliminary goals of our research were: (i) to set up this method, based on the detection of radiation induced 2-dodecylcyclobutanones (2-DCB) in pork muscle samples and (ii) to check the microbiological efficacy of the treatment. The main objective was to render the GC/MS a quantitative technique for dose estimation, through the measurement of the 2-DCB concentration in the irradiated sample. Our results show that the reduction of the microbial population is substantially reduced even at 2 kGy, and that a clear identification of irradiated samples can be achieved also one month after irradiation at 2 kGy in frozen-stored samples. The 2-DCB concentration showed a linear dependence on dose in the range 1−10 kGy, no matter the origin of the sample; a unique calibration function was obtained, that allowed dose estimation in irradiated pork samples. A retrospective evaluation on the quality of the treatment could be carried out this way.
- Published
- 2008
20. Antibiotic-Resistant Gram Negative Bacilli in Meals Delivered at a General Hospital, Italy
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Mammina C, Alberto Firenze, Maria Rosa Anna Plano, Sonia Sciortino, Anna Maria Di Noto, Plano, MRA, Di Noto, AM, Firenze, A, Sciortino, S, and Mammina, C
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Microbiology (medical) ,Gram-negative bacteria ,Article Subject ,medicine.drug_class ,Antibiotics ,Drug resistance ,Microbiology ,lcsh:Infectious and parasitic diseases ,hopsital catering ,Antibiotic resistance ,Virology ,medicine ,lcsh:RC109-216 ,gram negative bacilli ,Gram ,drug resistance ,biology ,business.industry ,food and beverages ,biology.organism_classification ,RAPD ,Multiple drug resistance ,Infectious Diseases ,Parasitology ,business ,Food contaminant ,Research Article - Abstract
This study aimed at detecting the presence of antibiotic-resistant Gram-negatives in samples of meals delivered at the University General Hospital of Palermo, Italy. Antibiotic resistant Gram negatives were isolated in July—September 2007 ffrom cold dishes and food contact surfaces and utensils. Bacterial strains were submitted to susceptibility test and subtyped by random amplification of polymorphic DNA (RAPD). Forty-six of 55 (83.6%) food samples and 14 of 17 (82.3%) environmental swabs were culture positive for Gram negative bacilli resistant to at least one group of antibacterial drugs. A total of 134 antibiotic resistant strains, 51 fermenters and 83 non-fermenters, were recovered. Fermenters and non-fermenters showed frequencies as high as 97.8% of resistance to two or more groups of antibiotics and non fermenters were 28.9% resistant to more than three groups. Molecular typing detected 34 different profiles among the fermenters and 68 among the non-fermenters. Antibiotic resistance was very common among both fermenters and non-fermenters. However, the wide heterogeneity of RAPD patterns seems to support a prominent role of cross-contamination rather than a clonal expansion of a few resistant isolates. A contribution of commensal Gram negatives colonizing foods to a common bacterial resistance pool should not been overlooked.
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- 2009
21. Gas chromatographic/mass spectrometric and microbiological analyses on irradiated chiken
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Maria Brai, Maria Cristina D'Oca, S. Giuffrida, Mirella Ferrugia, Aldo Parlato, Antonio Bartolotta, Luigi Tranchina, A Di Noto, S. Caracappa, Pasquale Agozzino, E. Calderaro, Giuseppe Avellone, PARLATO A, CALDERARO E, BARTOLOTTA A, D'OCA MC, GIUFFRIDA SA, BRAI M, TRANCHINA L, AGOZZINO P, AVELLONE G, FERRUGIA M, DI NOTO AM, and CARACAPPA S
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Irradiated food ,education.field_of_study ,Gas chromatography ,Radiation ,Chromatography ,Chemistry ,Population ,Food preservation ,Radiation treatment ,Mass spectrometry ,Shelf life ,Ionizing radiation ,Food irradiation ,Irradiation ,education - Abstract
Ionizing radiation is widely used as treatment technique for food preservation. It involves among others reduction of microbial contamination, disinfestations, sprout inhibition and extension of shelf life of food. However, the commercialization of irradiated food requires the availability of reliable methods to identify irradiated foodstuffs. In this paper, we present results on the application to irradiated chicken of this method, based on the detection, in muscle and skin samples, of the peaks of ions 98 Da and 112 Da, in a ratio approximately 4:1, typical of radiation induced 2-dodecylcyclobutanones (2-DCB). Aim of the work was also to study the time stability of the measured parameters in samples irradiated at 3 and 5 kGy, and to verify the efficacy of the treatment from a microbiological point of view. Our results show that, one month after irradiation at 3 kGy, the method is suitable using the skin but not the muscle, while the measured parameters are detectable in both samples irradiated at 5 kGy. The microbial population was substantially reduced even at 3 kGy. © 2007 Elsevier Ltd. All rights reserved.
- Published
- 2007
22. Pheno-genotyping of Salmonella enterica serotype Enteritidis isolates identified in Sicily during a reemergence period
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A M Di Noto, Caterina Mammina, Antonino Nastasi, Alberto Ricci, MAMMINA C, DI NOTO AM, RICCI A, and NASTASI A
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Serotype ,Salmonella ,Genotype ,Salmonella enteritidis ,Eggs ,Biology ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Microbiology ,Disease Outbreaks ,medicine ,Pulsed-field gel electrophoresis ,Animals ,Humans ,Typing ,Sicily ,Phylogeny ,Phage typing ,Bacterial Typing Techniques, Eggs microbiology, Plasmids genetics, Salmonella Food, oisoning epidemiology, Salmonella enteritidis isolation & purification ,Molecular Epidemiology ,Molecular epidemiology ,biology.organism_classification ,Virology ,Bacterial Typing Techniques ,Electrophoresis, Gel, Pulsed-Field ,Phenotype ,Salmonella enterica ,Food Microbiology ,Animal Science and Zoology ,Salmonella Food Poisoning ,Chickens ,Food Science ,Plasmids - Abstract
After an upward trend paralleling that occurring in most European countries, including Italy, since October 2002 Salmonella enterica serotype Enteritidis (S. Enteritidis) has again gained the first position among outbreak and sporadic human isolates of Salmonella in Sicily. Because phage typing of S. Enteritidis has many technical and epidemiological limitations and molecular methods have proved to be poorly discriminative for this organism, multiple typing, using phage typing together with pulsed field gel electrophoresis (PFGE) and plasmid profiling on a sample of fifty human and poultry isolates identified during the period October 2002 to May 2003 in Sicily, was chosen as the most valuable strategy to explore key features of this new epidemic wave. Although the limited number of strains imposes a cautious interpretation of the results, an apparently increasing phage type heterogeneity has emerged with rise in PT6 as the more striking event. While PFGE has confirmed the findings by other authors about the close genetic homogeneity between PT4 and PT6, plasmid profiling has provided discriminative patterns for PT6 strains. Combined phenotypic and genotypic profiles are necessary for epidemiological studies and public health investigations on S. enteritidis.
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- 2005
23. VanB-VanC1 Enterococcus gallinarum, Italy
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Caterina Mammina, Antonino Nastasi, Antonella Costa, Anna Maria Di Noto, MAMMINA C, DI NOTO AM, COSTA A, and NASTASI A
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Microbiology (medical) ,Epidemiology ,education ,letter ,lcsh:Medicine ,Microbial Sensitivity Tests ,Enteococcus gallinarum ,vanB-vanC1 ,lcsh:Infectious and parasitic diseases ,Microbiology ,chemistry.chemical_compound ,Enterococcus gallinarum ,Bacterial Proteins ,Multiplex polymerase chain reaction ,medicine ,Animals ,media_common.cataloged_instance ,lcsh:RC109-216 ,Peptide Synthases ,European union ,Letters to the Editor ,media_common ,biology ,Teicoplanin ,poultry ,lcsh:R ,Avoparcin ,Vancomycin Resistance ,biochemical phenomena, metabolism, and nutrition ,vancomycin-resistant enterococci ,bacterial infections and mycoses ,biology.organism_classification ,Glycopeptide ,Infectious Diseases ,Enterococcus ,chemistry ,Italy ,Vancomycin ,Chickens ,medicine.drug - Abstract
To the Editor: We report detecting a vanB determinant in Enterococcus gallinarum in poultry in Italy. High-level vanA-mediated glycopeptide resistance has been described for E. gallinarum and E. casseliflavus (1–4), and vanB-mediated vancomycin resistance has been frequently described for E. faecalis and E. faecium. However, vanB-mediated resistance in isolates of E. gallinarum has been described only in sporadic nosocomial cases of infection or colonization (5,6). In January 2005, a study of contamination by foodborne organisms in slaughtered broiler carcasses was conducted in Sicily. To detect glycopeptide-resistant enterococci (GRE), each carcass was placed in a bag with 100 mL sterile buffered peptone water and shaken vigorously for 60 sec. After overnight incubation at 37°C, 0.5 mL rinsate was added in duplicate to 5 mL ethyl violet azide broth (Oxoid, Basingstoke, United Kingdom) with 4 mg/L vancomycin. Broth cultures were further incubated at 37°C for 48 h, and 0.1 mL aliquots were spread onto duplicate plates of VRE (commercial denomination product, Oxoid) agar. A vancomycin-resistant isolate of E. gallinarum was identified in a carcass from a broiler farm in eastern Sicily. The biochemical tests of API 20 Strep (bioMerieux, Marcy l'Etoile, France) and motility test at 30°C were used to characterize the isolate at the species level. The MICs of vancomycin and teicoplanin were 64 μg/mL and 1 μg/mL, respectively. The isolate was subjected to a multiplex polymerase chain reaction followed by an endonuclease cleavage of amplicons by MspI (Invitrogen, Carlsbad, CA, USA) as previously described (7) to detect van gene determinants; this process demonstrated a simultaneous presence of vanC1 and vanB determinants. E. gallinarum and the other motile enterococci are thought to infrequently cause infection. However, the recent involvement of vanC1-vanA E. gallinarum in person-to-person spread in a long-term-care facility (8) and in an intensive care unit (2), along with identification of vanC1-vanB isolates in some patients treated with prolonged courses of glycopeptides (5,6), suggests reassessment of their possible pathogenic role. For the first time, 1 isolate of E. gallinarum has been found harboring the vanB gene in poultry. Our findings confirm that E. gallinarum can capture the genetic determinants of high-level glycopeptide resistance, probably under selective pressure conditions that do not permit survival of a host organism with constitutive low-level resistance (3). Previous studies have demonstrated that E. gallinarum can transfer these determinants to E. faecium by conjugation (2). The role of food animals as reservoirs of GRE and the causes of their persistently high prevalence in poultry carcasses in some European countries are being investigated (9). Moreover, the public health risk associated with consumer exposure to GRE when handling raw animal foods is poorly understood. In Europe, the food chain is thought to be the major source of GRE since avoparcin was used as a food additive for animals until the European Union ban in 1997. Previous studies in Italy showed that avoparcin withdrawal successfully reduced GRE contamination of poultry meat products (10). However, our finding, 7 years after the European Union ban, highlights that resistance genotypes in motile enterococci should be closely monitored (11).
- Published
- 2005
24. A food borne outbreak of Salmonella enterica serotype Brandenburg as a hint to compare human, animal and food isolates identified in the years 2005-2009 in Italy
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Mammina, C., Aleo, A., Romanelli, G., Marconi, P., Anna Maria Di Noto, Donato, R., Nastasi, A., Mammina, C, Aleo, A, Romanelli, G, Marconi, P, Di Noto, AM, Donato, R, and Nastasi, A
- Subjects
Salmonella Brandenburg, molecular epidemiology, foodborne outbreak ,Settore MED/42 - Igiene Generale E Applicata - Abstract
Introduction. There are only a few reported cases of Salmonella enterica serotype Brandenburg foodborne outbreaks in the literature. In Italy Brandenburg is consistently present among the top-ten serotypes from human source, but at low prevalences. Methods. Fifty-five S. Brandenburg isolates from human, animal, environmental and food sources, including twelve isolates from a foodborne outbreak, were genotyped by PFGE. Results and Discussion. Eight pulsogroups and 19 pulsotypes were detected, with a unique pulsotype being attributed to the outbreak strains. Molecular subtyping can reliably complement the epidemiological investigations. Moreover, mapping molecular types of Salmonella isolates from human and non-human source may greatly contribute to risk assessment, by tracking possible animal sources, so improving cost-effectiveness of the prevention and control strategies.
25. Assessment of contamination of Salmonella spp. in imported black pepper and sesame seed and salmonella inactivation by gamma irradiation.
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D'Oca MC, Di Noto AM, Bartolotta A, Parlato A, Nicastro L, Sciortino S, and Cardamone C
- Abstract
This study shows the frequency of seeds samples contaminated by Salmonella spp. collected randomly from local markets; on 30 black pepper sample no contaminated sample was found while Salmonella spp. was detected in 3 of 36 (8.3%) analyzed sesame samples; three different serotypes were identified: S. Montevideo, S. Stanleyville e S. Tilene. The efficacy of gamma irradiation to inactivate Salmonella Montevideo in black pepper and sesame irradiated between 1 and 5 kGy was evaluated. 3 kGy is sufficient to reduce of 3-4 log CFU/g; whereas 5 kGy have been need to reduce 5.5-6 log CFU/g for samples of black pepper and sesame. No statistically significant differences were found between black pepper and sesame., Competing Interests: Conflict of interest: The authors declare no potential conflict of interests., (©Copyright: the Author(s).)
- Published
- 2021
- Full Text
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26. Extended-Spectrum ß-Lactamase, AmpC-Producing, and Fluoroquinolone-Resistant Escherichia coli in Retail Broiler Chicken Meat, Italy.
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Ghodousi A, Bonura C, Di Noto AM, and Mammina C
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- Animals, Anti-Bacterial Agents pharmacology, Bacterial Proteins genetics, Bacterial Proteins metabolism, Cephalosporins pharmacology, Ciprofloxacin pharmacology, Escherichia coli genetics, Escherichia coli metabolism, Food Microbiology, Genotyping Techniques, Italy, Phylogeny, Plasmids genetics, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Virulence Factors genetics, beta-Lactamases genetics, beta-Lactamases metabolism, Chickens microbiology, Drug Resistance, Multiple, Bacterial, Escherichia coli isolation & purification, Food Contamination analysis, Poultry microbiology
- Abstract
Background: Globally, antimicrobial drug-resistant Escherichia coli is among the most common etiological agents of invasive disease in humans. In Europe, increasing proportions of infections due to third-generation cephalosporins and/or fluoroquinolone-resistant extraintestinal pathogenic E. coli (ExPEC) strains are reported. E. coli from poultry are those more closely linked to human E. coli, but lack of reliable data makes it difficult to assess the attributable risk of different food sources. In the present study, our objective was to investigate the antimicrobial resistance profile, phylogenetic background, and virulence factors of E. coli isolates from broiler chicken meat sold at retail in Palermo, Italy., Materials and Methods: Isolation of multidrug resistant (MDR) E. coli was performed during April-December 2013 on a total of 163 chicken meat samples. Susceptibility to a panel of nine antimicrobial agents was determined. PCR assays were carried out to detect extended-spectrum β-lactamase (ESBL), plasmid-mediated AmpC β-lactamase, and plasmid-mediated quinolone resistance (PMQR) genes, phylogenetic group, and ExPEC-associated traits. A single nucleotide polymorphism (SNP) PCR was done to detect E. coli sequence type (ST)131., Results: One hundred thirty-four isolates from 109 meat samples were MDR. B1 was the most prevalent phylogenetic group (47.8%), followed by groups D (25.4%), A (22.3%), and B2 (4.5%). ESBLs and AmpC β-lactamases were detected by PCR in 132 (98.5%) and 15 (11.2%) isolates. PMQR determinants were detected in 122 (91%) isolates. Twenty-two MDR isolates met the molecular definition of ExPEC. SNP-PCR results confirmed that four B2 isolates were ST131. Enterobacterial Repetitive Intergenic Consensus sequence-PCR analysis showed a large heterogeneity with 55 unique profiles and 31 clusters including 2-4 isolates., Conclusions: An alarmingly high prevalence of MDR E. coli from broiler chicken meat is evident in our geographic area. The ongoing use of antimicrobial drugs in livestock should be urgently restricted, particularly in the poultry sector.
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- 2015
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27. Assessment of the microbiological quality of fresh produce on sale in Sicily, Italy: preliminary results.
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Cardamone C, Aleo A, Mammina C, Oliveri G, and Di Noto AM
- Abstract
Background: Fresh produce occupies an increasingly important place in the human food supply because of its health-promoting nutritional properties. Most fresh produce is eaten raw or after minimal processing and, consequently, pathogen contamination can represent a serious health risk. There has been an increase in foodborne outbreaks and cases associated with fresh produce, but literature data about the prevalence of pathogen contamination are inconsistent. This study was undertaken to assess the hygienic quality and the prevalence of the most common bacterial pathogens in fresh produce sold in retail markets in Sicily. A total of 125 samples of different types of vegetables were examined by standardized microbiological methods., Results: The aerobic mesophilic count ranged between 2 log and 7 log cfu g(-1) and the Enterobacteriaceae counts between < 1 log and 6 log cfu g(-1), with statistically significant differences between unprocessed and minimally processed products (p < 0.05). Escherichia coli was detected only in leaf vegetables at a concentration of 2 log - 3 log cfu g(-1). Enterococci were found at a concentration of 2 log - 4 log cfu g(-1). Coagulase positive Staphylococci and sulphite-reducing Clostridia were not detected in any sample. Three samples tested positive for Listeria monocytogenes, Yersinia enterocolitica and Salmonella veneziana., Conclusion: Our study provides updated data on the microbiological quality of retail vegetables and confirms the need to implement strategies to increase microbial safety of fresh produce.
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- 2015
- Full Text
- View/download PDF
28. Prevalence of sensitization to Anisakis simplex among professionally exposed populations in Sicily.
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Mazzucco W, Lacca G, Cusimano R, Provenzani A, Costa A, Di Noto AM, Massenti MF, Leto-Barone MS, Lorenzo GD, and Vitale F
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- Adult, Animals, Cross-Sectional Studies, Female, Fisheries statistics & numerical data, Humans, Hypersensitivity immunology, Immunoglobulin E blood, Immunoglobulin E immunology, Male, Middle Aged, Occupational Diseases epidemiology, Prevalence, Sicily epidemiology, Anisakis immunology, Hypersensitivity epidemiology, Occupational Exposure statistics & numerical data
- Abstract
Anisakis simplex (AS) is a cause of allergic sensitization and potential occupational risk is suggested in fishermen and workers assigned to fish processing and sale. A cross-sectional study was conducted in order to assess possible health effects of occupational exposure to AS in workers recruited from western Sicily fisheries sector. Social, demographic, and occupation-related data were collected. Serum total immunoglobulin E (IgE) and specific IgE levels to AS (threshold >0.35 kU/L) were determined by an fluoroimmunoassay technique. Ninety-four subjects with potential occupational exposure (fishmongers, fishermen, fish industry employees) were recruited. Specific AS IgEs were detected in 20.2% of the study population. AS IgE seroprevalence was elevated 6.7-fold (p = .03) among fishermen/sailors compared with fish industry workers. The study suggested the importance to adopt specific prevention strategies against exposure to AS in the occupational setting.
- Published
- 2012
- Full Text
- View/download PDF
29. A food borne outbreak of Salmonella enterica serotype Brandenburg as a hint to compare human, animal and food isolates identified in the years 2005-2009 in Italy.
- Author
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Mammina C, Aleo A, Romanelli G, Marconi P, Di Noto AM, Donato R, and Nastasi A
- Subjects
- Adolescent, Adult, Aged, Animals, DNA, Bacterial genetics, Electrophoresis, Gel, Pulsed-Field, Female, Humans, Italy epidemiology, Male, Middle Aged, Population Surveillance methods, Retrospective Studies, Salmonella enterica genetics, Young Adult, DNA, Bacterial isolation & purification, Disease Outbreaks statistics & numerical data, Food Microbiology methods, Salmonella Food Poisoning epidemiology, Salmonella Food Poisoning microbiology, Salmonella enterica isolation & purification, Serotyping methods
- Abstract
Introduction: There are only a few reported cases of Salmonella enterica serotype Brandenburg foodborne outbreaks in the literature. In Italy Brandenburg is consistently present among the top ten serotypes from human source, but at low prevalences., Materials and Methods: Fifty-five S. Brandenburg isolates from human, animal, environmental and food sources, including twelve isolates from a foodborne outbreak, were genotyped by Pulsed-Field Gel Electrophoresis (PFGE)., Results and Discussion: Eight pulsogroups and 19 pulsotypes were detected, with a unique pulsotype being attributed to the outbreak strains. Molecular subtyping can reliably complement the epidemiological investigations. Moreover, mapping molecular types of Salmonella isolates from human and non-human source may greatly contribute to risk assessment, by tracking possible animal sources, so improving cost-effectiveness of the prevention and control strategies.
- Published
- 2011
30. Antibiotic-resistant gram negative bacilli in meals delivered at a general hospital, Italy.
- Author
-
Plano MR, Di Noto AM, Firenze A, Sciortino S, and Mammina C
- Abstract
This study aimed at detecting the presence of antibiotic-resistant Gram-negatives in samples of meals delivered at the University General Hospital of Palermo, Italy. Antibiotic resistant Gram negatives were isolated in July-September 2007 ffrom cold dishes and food contact surfaces and utensils. Bacterial strains were submitted to susceptibility test and subtyped by random amplification of polymorphic DNA (RAPD). Forty-six of 55 (83.6%) food samples and 14 of 17 (82.3%) environmental swabs were culture positive for Gram negative bacilli resistant to at least one group of antibacterial drugs. A total of 134 antibiotic resistant strains, 51 fermenters and 83 non-fermenters, were recovered. Fermenters and non-fermenters showed frequencies as high as 97.8% of resistance to two or more groups of antibiotics and non fermenters were 28.9% resistant to more than three groups. Molecular typing detected 34 different profiles among the fermenters and 68 among the non-fermenters. Antibiotic resistance was very common among both fermenters and non-fermenters. However, the wide heterogeneity of RAPD patterns seems to support a prominent role of cross-contamination rather than a clonal expansion of a few resistant isolates. A contribution of commensal Gram negatives colonizing foods to a common bacterial resistance pool should not been overlooked.
- Published
- 2009
- Full Text
- View/download PDF
31. VanB-VanC1 Enterococcus gallinarum, Italy.
- Author
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Mammina C, Di Noto AM, Costa A, and Nastasi A
- Subjects
- Animals, Chickens, Italy, Microbial Sensitivity Tests, Bacterial Proteins drug effects, Enterococcus drug effects, Peptide Synthases drug effects, Vancomycin Resistance genetics
- Published
- 2005
- Full Text
- View/download PDF
32. Pheno-genotyping of Salmonella enterica serotype Enteritidis isolates identified in Sicily during a reemergence period.
- Author
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Mammina C, Noto AM, Ricci A, and Nastasi A
- Subjects
- Animals, Chickens, Disease Outbreaks, Electrophoresis, Gel, Pulsed-Field, Food Microbiology, Genotype, Humans, Molecular Epidemiology, Phenotype, Phylogeny, Salmonella enteritidis classification, Salmonella enteritidis genetics, Sicily epidemiology, Bacterial Typing Techniques, Eggs microbiology, Plasmids genetics, Salmonella Food Poisoning epidemiology, Salmonella enteritidis isolation & purification
- Abstract
After an upward trend paralleling that occurring in most European countries, including Italy, since October 2002 Salmonella enterica serotype Enteritidis (S. Enteritidis) has again gained the first position among outbreak and sporadic human isolates of Salmonella in Sicily. Because phage typing of S. Enteritidis has many technical and epidemiological limitations and molecular methods have proved to be poorly discriminative for this organism, multiple typing, using phage typing together with pulsed field gel electrophoresis (PFGE) and plasmid profiling on a sample of fifty human and poultry isolates identified during the period October 2002 to May 2003 in Sicily, was chosen as the most valuable strategy to explore key features of this new epidemic wave. Although the limited number of strains imposes a cautious interpretation of the results, an apparently increasing phage type heterogeneity has emerged with rise in PT6 as the more striking event. While PFGE has confirmed the findings by other authors about the close genetic homogeneity between PT4 and PT6, plasmid profiling has provided discriminative patterns for PT6 strains. Combined phenotypic and genotypic profiles are necessary for epidemiological studies and public health investigations on S. enteritidis.
- Published
- 2004
- Full Text
- View/download PDF
33. Clonal circulation of Salmonella enterica serotype Heidelberg in Italy?
- Author
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Mammina C, Talini M, Pontello M, Di Noto AM, and Nastasi A
- Subjects
- Animals, Chickens microbiology, Child, DNA, Bacterial classification, DNA, Bacterial genetics, Drug Resistance, Multiple, Bacterial, Electrophoresis, Gel, Pulsed-Field methods, Genotype, Humans, Italy epidemiology, Male, Microbial Sensitivity Tests methods, Molecular Epidemiology classification, Molecular Epidemiology methods, Phenotype, Population Surveillance methods, Salmonella Food Poisoning epidemiology, Salmonella Infections classification, Salmonella Infections metabolism, Salmonella enterica drug effects, Salmonella enterica genetics, Serotyping, Salmonella Infections epidemiology, Salmonella Infections genetics, Salmonella enterica classification, Salmonella enterica isolation & purification
- Abstract
Phenotypic and genetic characteristics of 21 strains of Salmonella serotype Heidelberg isolated in the years 1999-2003 from different sources in Italy were studied. Susceptibility patterns, plasmid analysis, and PFGE were used as epidemiological markers. Although non-homogeneous drug resistance patterns and plasmid profiles had been detected, PFGE patterns suggest the hypothesis of a nationwide clonal spread of this serotype associated with poultry.
- Published
- 2003
- Full Text
- View/download PDF
34. Tibial cortical bone peg fixation in osteochondritis dissecans of the knee.
- Author
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Slough JA, Noto AM, and Schmidt TL
- Subjects
- Adolescent, Adult, Follow-Up Studies, Humans, Knee Joint pathology, Magnetic Resonance Imaging, Osteochondritis Dissecans diagnosis, Retrospective Studies, Tibia transplantation, Bone Transplantation methods, Knee Joint surgery, Osteochondritis Dissecans surgery
- Abstract
Ten knees in nine patients with large, symptomatic osteochondritis dissecans lesions of the knee were treated by cortical bone peg fixation. The follow-up time averaged 2.9 years (range, 0.9-6.6 years). The average age was 19.5 years (range, 15-33 years). The knees were evaluated and rated clinically and roentgenographically. Magnetic resonance (MR) imaging of nine knees was obtained at the follow-up examination to evaluate lesion and bone peg incorporation, evidence of lesion loosening, and the articular cartilage. Eight good to excellent, one fair, and one poor result were obtained clinically. MR imaging showed 33% of knees had poor lesion cartilage and 44% had partial or poor lesion incorporation possibly consistent with loosening. Complications included four partial defect healings, one tibial donor graft-site fracture, and one bone peg loosening. Four symptomatic knees had follow-up arthroscopy at an average of 1.8 years postsurgery and findings included four loose bodies, one loose peg, one meniscal tear, and one symptomatic hypertrophic synovium. MR imaging was a useful tool in evaluating lesion stability, articular cartilage quality, lesion and bone peg incorporation, the menisci, and cruciate ligaments. Bone peg fixation of large osteochondritis dissecans defects of the knee offers a physiologic approach to treatment of this lesion in nearly skeletally mature or skeletally mature patients.
- Published
- 1991
35. Epidemiological evaluation by rRNA-DNA hybridization of strains of Salmonella enterica subsp. enterica serovar Abortusovis isolated in southern Italy in the years 1981-1989.
- Author
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Nastasi A, Mammina C, Villafrate MR, Caracappa S, Di Noto AM, and Balbo R
- Subjects
- Abortion, Veterinary epidemiology, Animals, Azides, Biotin analogs & derivatives, DNA Fingerprinting, Drug Resistance, Microbial, Escherichia coli genetics, Female, Nucleic Acid Hybridization, Pregnancy, Salmonella drug effects, Salmonella genetics, Salmonella isolation & purification, Salmonella Infections, Animal epidemiology, Sheep microbiology, Sheep Diseases epidemiology, Sicily epidemiology, Species Specificity, Abortion, Veterinary microbiology, Bacterial Typing Techniques, DNA, Bacterial genetics, DNA, Ribosomal genetics, RNA, Bacterial genetics, RNA, Ribosomal genetics, Salmonella classification, Salmonella Infections, Animal microbiology, Sheep Diseases microbiology
- Abstract
Salmonella enterica subsp. enterica serovar Abortusovis is a major agent of abortion of the sheep and is firmly established, although at low prevalence, in Sicily. This paper describes the application rDNA gene restriction pattern fingerprinting to investigate relatedness among 7 serovar Abortusovis strains isolated at the "Istituto Zooprofilattico Sperimentale" of Sicily and 29 isolates identified at the Southern Italy Centre of Enterobacteriaceae between 1981 and 1989. Although Abortusovis serovar has exhibited a remarkable degree of homogeneity, genomic DNA polymorphisms, that have emerged, suggest possible importation of bacterial clones from different geographic areas.
- Published
- 1991
36. Volumetric three-dimensional computed tomography for acute calcaneus fractures: preliminary report.
- Author
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Carr JB, Noto AM, and Stevenson S
- Subjects
- Calcaneus diagnostic imaging, Humans, Calcaneus injuries, Fractures, Bone diagnostic imaging, Tomography, X-Ray Computed methods
- Abstract
Volumetric three-dimensional (3-D) computed tomographic reconstructions were performed on three cadaver specimens before and after fracture for 16 acute calcaneal fractures. This "second-generation" 3-D reconstruction technique produced accurate, detailed images of the extra-articular fracture anatomy. Use of this method provided visualization of specific fracture component displacement otherwise unappreciated prior to surgery. In two cases, vertical splits in the superomedial fragment were demonstrated to best advantage by this 3-D technique.
- Published
- 1990
- Full Text
- View/download PDF
37. Infections of the musculoskeletal system: high-field-strength MR imaging.
- Author
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Beltran J, Noto AM, McGhee RB, Freedy RM, and McCalla MS
- Subjects
- Humans, Abscess diagnosis, Connective Tissue Diseases diagnosis, Joint Diseases diagnosis, Magnetic Resonance Spectroscopy, Osteomyelitis diagnosis
- Abstract
Twenty-two patients with clinical findings consistent with osteomyelitis, soft-tissue infection, or both were studied with magnetic resonance (MR) imaging at 1.5 T. Another 15 patients with joint effusion but no clinical or laboratory signs of infection served as controls. Soft-tissue abscesses, osteomyelitis, joint and tendon sheath effusion, and cellulitis were well depicted on MR imaging, allowing the correct diagnosis of presence and extent of infection in all but two cases. MR imaging was as sensitive as technetium-99m methylene diphosphonate bone scintigraphy in demonstrating osteomyelitis and was more specific and more sensitive than other scintigraphic techniques in demonstrating soft-tissue infections, primarily because of its superior spatial resolution. Computed tomography, performed in seven cases, was as accurate as MR imaging in demonstrating bone and soft-tissue infections. Infected and noninfected synovial effusions had the same signal intensity, but associated findings such as soft-tissue fluid collections or osteomyelitis made the distinction possible.
- Published
- 1987
- Full Text
- View/download PDF
38. Ankle: surface coil MR imaging at 1.5 T.
- Author
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Beltran J, Noto AM, Mosure JC, Shamam OM, Weiss KL, and Zuelzer WA
- Subjects
- Ankle pathology, Ankle Injuries, Ankle Joint pathology, Humans, Ankle anatomy & histology, Joint Diseases pathology, Magnetic Resonance Spectroscopy
- Abstract
High-field surface coil magnetic resonance (MR) images were obtained of 12 ankles: two from healthy volunteers, seven from patients, and three from fresh cadavers. The cadaver ankles were sectioned in the coronal, sagittal, and axial planes for direct comparison with the MR images. Plain film confirmation of pathologic conditions was obtained in all patients, and five underwent arthroscopy or surgery, or both. MR imaging provided excellent delineation of ligaments and cartilaginous structures in all cases.
- Published
- 1986
- Full Text
- View/download PDF
39. Rupture of the posterior tibial tendon: CT and surgical findings.
- Author
-
Rosenberg ZS, Jahss MH, Noto AM, Shereff MJ, Cheung Y, Frey CC, and Norman A
- Subjects
- Adult, Aged, Female, Humans, Male, Middle Aged, Rupture, Tendon Injuries surgery, Ankle Joint diagnostic imaging, Tendon Injuries diagnostic imaging, Tomography, X-Ray Computed
- Abstract
Computed tomography (CT) was performed in 42 patients with 49 clinically suspected tears of the posterior tibial tendon. Twenty-eight of the 49 suspected tears were subsequently surgically explored and repaired. Three patterns of tendon abnormalities were recognized on CT scans: type I-intact, hypertrophied, heterogeneous tendon; type II-attenuated tendon; and type III-absence of a portion of a tendon. Types I and II correlated with partial rupture seen during surgery, and type III correlated with complete rupture of the tendon. CT findings were accurate in 96% of the patients who underwent surgery. In four cases (14%), tendon rupture was seen on CT scans, but the extent of the injury was underestimated and the rupture was misclassified. Reactive periostitis of the distal tibia was seen in 71% of diseased tendons and may represent an important factor in the diagnosis of tendon rupture.
- Published
- 1988
- Full Text
- View/download PDF
40. MR imaging of the ankle: normal variants.
- Author
-
Noto AM, Cheung Y, Rosenberg ZS, Norman A, and Leeds NE
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Ankle Joint anatomy & histology, Magnetic Resonance Imaging
- Abstract
Thirty asymptomatic ankles were studied with high-resolution surface coil magnetic resonance (MR) imaging to identify normal structures. Several normal variants were demonstrated, including (a) Cortical irregularity of the posterior tibiotalar joint (should not be mistaken for osteonecrosis) in 27 of 30 cases; (b) normal posterior talofibular ligament with irregular and frayed superior edge simulating a tear in 13 of 30 cases; (c) normal navicular insertion of the posterior tibial tendon with heterogeneous signal intensity in 14 of 30 cases; (d) deltoid ligament inhomogeneity in seven of ten cases; and (e) fluid in the shared peroneal tendons sheath (may be confused with a longitudinal tendon tear) in three of 30 cases. Ankle imaging with the use of MR is still a relatively new procedure. Further investigation is needed to better define normal anatomy and normal variants.
- Published
- 1989
- Full Text
- View/download PDF
41. Magnetic resonance imaging: failure to demonstrate spinal arteriovenous malformations in four patients.
- Author
-
Shah DC, James R, Ashraf A, Noto AM, and Shull SY
- Subjects
- Adult, Evaluation Studies as Topic, Female, Humans, Male, Myelography, Arteriovenous Malformations diagnosis, Magnetic Resonance Imaging, Spinal Cord blood supply
- Abstract
Magnetic resonance imaging (MRI), often useful for detecting spinal lesions, was unsuccessful in revealing spinal arteriovenous malformations in four patients, as recorded here. Patients with persistent neurological symptoms after "normal" MRI scans should undergo additional imaging procedures.
- Published
- 1989
42. Rupture of posterior tibial tendon: CT and MR imaging with surgical correlation.
- Author
-
Rosenberg ZS, Cheung Y, Jahss MH, Noto AM, Norman A, and Leeds NE
- Subjects
- Adult, Female, Humans, Male, Middle Aged, Rupture, Tendon Injuries surgery, Ankle, Magnetic Resonance Imaging, Tendon Injuries diagnosis, Tomography, X-Ray Computed
- Abstract
Computed tomography (CT) and magnetic resonance (MR) imaging were performed in 32 cases of clinically suspected chronic tears of the posterior tibial tendon. Surgery was performed in 22 patients (69%). Each case was classified radiographically and surgically as normal or a type 1, type 2, or type 3 rupture. The sensitivity and specificity of CT were 90% and 100%, respectively, while those of MR imaging were 95% and 100%. The accuracy in detecting ruptures was 91% for CT and 96% for MR imaging. The overall accuracy, which reflected the percentage of cases correctly diagnosed as well as those correctly classified, was 59% for CT and 73% for MR imaging. Although the differences between the CT and MR imaging parameters were not statistically significant (possibly due to the small population), the results suggest that MR imaging is the method of choice for detecting ruptures of the posterior tibial tendon. MR imaging provided greater definition of tendon outline, vertical splits, synovial fluid, edema, and degenerated tissue. CT was superior to MR imaging in showing associated bone abnormalities such as periostitis, subtalar osteoarthritis, and subtalar dislocation.
- Published
- 1988
- Full Text
- View/download PDF
43. Tumors of the osseous spine: staging with MR imaging versus CT.
- Author
-
Beltran J, Noto AM, Chakeres DW, and Christoforidis AJ
- Subjects
- Humans, Retrospective Studies, Spinal Neoplasms diagnostic imaging, Magnetic Resonance Spectroscopy, Spinal Neoplasms diagnosis, Tomography, X-Ray Computed
- Abstract
Twelve patients with tumors involving the osseous spine were evaluated with magnetic resonance (MR) imaging and computed tomography (CT), six with and six without intrathecal injection of contrast material. MR imaging was found to be superior to CT without contrast material and equal to CT with contrast material in delineating the anatomic relationships of the tumors, including bone involvement, spinal canal invasion, paraspinal soft-tissue extension, and vascular involvement. Since MR imaging can provide most of the anatomic information necessary for treatment planning, intrathecal injection of contrast material can be avoided in most instances when evaluating tumor involvement of the osseous spine. However, CT without contrast medium was superior to MR imaging in showing critical cortical bone destruction and calcified tumor matrix.
- Published
- 1987
- Full Text
- View/download PDF
44. Meniscal tears: MR demonstration of experimentally produced injuries.
- Author
-
Beltran J, Noto AM, Mosure JC, Bools JC, Zuelzer W, and Christoforidis AJ
- Subjects
- Animals, Disease Models, Animal, Knee Joint pathology, Magnetic Resonance Spectroscopy, Swine, Knee Injuries diagnosis
- Abstract
Magnetic resonance (MR) imaging was performed using a 1.5-T magnet on eight immature swine knees with surgically produced vertical and horizontal meniscal tears. Three radiologists, interpreting the images independently, detected all tears. Tears in the middle portion of the meniscus were best seen on sagittal views, while lesions of the anterior and posterior horns of the meniscus were best seen on coronal views. MR has several advantages over arthrography, the current diagnostic method for knee injuries: it is noninvasive, uses no ionizing radiation, and provides excellent soft-tissue contrast resolution.
- Published
- 1986
- Full Text
- View/download PDF
45. Tendons: high-field-strength, surface coil MR imaging.
- Author
-
Beltran J, Noto AM, Herman LJ, and Lubbers LM
- Subjects
- Animals, Cicatrix diagnosis, Humans, Postoperative Complications diagnosis, Rupture, Swine, Tendinopathy diagnosis, Tendon Injuries diagnosis, Tendons pathology, Magnetic Resonance Spectroscopy, Tendons anatomy & histology
- Abstract
High-resolution magnetic resonance (MR) images of the tendons of the hands, wrists, feet, and ankles of six healthy volunteers and six cadavers were obtained using receive-only surface coils and reduced-field-of-view imaging. Normal anatomy was identified and compared with gross anatomic sections of the six cadavers. Experimentally produced tears of the calcaneal (Achilles) tendon in domestic swine were identified on MR images. The hands and feet of 11 patients were examined, and a variety of pathologic lesions were identified, including acute posttraumatic rupture, acute tenosynovitis, chronic tendonitis, and postsurgical complications. MR imaging provides inherently greater soft-tissue contrast than any other currently available imaging modality. With the use of surface coils and reduced-field-of-view imaging to enhance spatial resolution, MR imaging has become a valuable tool for imaging tendons. Advantages over other available modalities include excellent depiction of anatomic detail, superior contrast resolution, and the potential for multiplanar imaging.
- Published
- 1987
- Full Text
- View/download PDF
46. Right ventricular aneurysm due to ischemic disease. Diagnosis by radionuclide angiography with localization of the site of PVC origin by phase analysis.
- Author
-
Shaffer PB, Bashore TM, Noto AM, Diblasio G, Olsen JO, and Schall SF
- Subjects
- Aged, Coronary Vessels diagnostic imaging, Electrocardiography, Heart Aneurysm diagnostic imaging, Heart Ventricles diagnostic imaging, Humans, Male, Radionuclide Angiography, Coronary Disease complications, Heart Aneurysm etiology
- Abstract
A case of right ventricular aneurysm due to coronary artery disease in a 69-year-old male with no prior history of surgery or chest trauma is reported. The presence of the aneurysm was diagnosed by gated radionuclide angiography and confirmed by contrast angiography. This is the first description of an isolated right ventricular aneurysm on the basis of ischemic disease. In addition, the patient had recurrent ventricular tachycardia and frequent premature ventricular contractions (PVC's). Using radionuclide angiographic phase analysis of the PVC's, their apparent origin was localized to the margin of the right ventricular aneurysm.
- Published
- 1987
- Full Text
- View/download PDF
47. CT demonstration of a ureteroinguinal hernia causing hydronephrosis.
- Author
-
Noto AM, Ragosin RJ, and Beltran J
- Subjects
- Aged, Hernia complications, Hernia diagnostic imaging, Hernia, Inguinal complications, Humans, Hydronephrosis diagnostic imaging, Male, Ureteral Diseases complications, Hernia, Inguinal diagnostic imaging, Hydronephrosis etiology, Tomography, X-Ray Computed, Ureteral Diseases diagnostic imaging
- Published
- 1985
48. Rheumatoid arthritis: MR imaging manifestations.
- Author
-
Beltran J, Caudill JL, Herman LA, Kantor SM, Hudson PN, Noto AM, and Baran AS
- Subjects
- Adult, Aged, Arthritis, Rheumatoid drug therapy, Arthrography, Chronic Disease, Female, Gold therapeutic use, Humans, Joints pathology, Male, Middle Aged, Time Factors, Arthritis, Rheumatoid diagnosis, Magnetic Resonance Spectroscopy methods
- Abstract
Radiologic assessment of the stage and treatment response of rheumatoid arthritis (RA) is based on the presence of bone erosions, joint-space narrowing, and osteoporosis. Most radiologic methods for staging RA lack interobserver correlation and are time consuming. Magnetic resonance (MR) imaging provides excellent depiction of soft-tissue abnormalities of the joints affected by RA, which allows detection of early changes. Nineteen joints of 17 patients with RA were studied with surface-coil MR imaging. Measurable abnormalities demonstrated by MR imaging but not clearly seen on plain radiographs included bone erosions, joint effusion, synovial sheath effusion, and cartilage irregularity and thinning. Seven patients of this group underwent MR imaging before and after 6 months of gold therapy. Four patients had significant interval changes on MR images that were not seen on plain radiographs. MR imaging may become a sensitive and objective method for quantitative assessment of the joint changes of RA.
- Published
- 1987
- Full Text
- View/download PDF
49. The knee: surface-coil MR imaging at 1.5 T1.
- Author
-
Beltran J, Noto AM, Mosure JC, Weiss KL, Zuelzer W, and Christoforidis AJ
- Subjects
- Humans, Knee Injuries diagnosis, Knee Joint pathology, Knee Joint anatomy & histology, Magnetic Resonance Spectroscopy
- Abstract
Seven normal knees (in five volunteers) and seven injured knees (in seven patients) were examined by high-resolution magnetic resonance (MR) imaging at 1.5 T with a surface coil. Seven medial meniscal tears, three anterior cruciate ligament tears, one posterior cruciate ligament avulsion, an old osteochondral fracture, femoral condylar chondromalacia, and one case of semimembranous tendon reinsertion were identified. MR images correlated well with recent double-contrast arthrograms or results of surgery. All tears were identified in both the sagittal and coronal planes. Because of its ability to demonstrate small meniscal lesions and ligamentous injuries readily, MR imaging with a surface coil may eventually replace the more invasive arthrography.
- Published
- 1986
- Full Text
- View/download PDF
50. Joint effusions: MR imaging.
- Author
-
Beltran J, Noto AM, Herman LJ, Mosure JC, Burk JM, and Christoforidis AJ
- Subjects
- Adult, Animals, Blood, Cartilage, Articular anatomy & histology, Cartilage, Articular pathology, Hemarthrosis diagnosis, Hip Joint pathology, Humans, Injections, Knee Joint pathology, Sodium Chloride, Swine, Exudates and Transudates, Hip Joint anatomy & histology, Joint Diseases diagnosis, Knee Joint anatomy & histology, Magnetic Resonance Spectroscopy, Synovial Fluid
- Abstract
Magnetic resonance (MR) images of the hips and knees of three healthy volunteers were reviewed and compared with images obtained from the hips of three patients with effusions. Comparison was also made with MR images obtained from two swine knees following the injections of saline in one and blood in the other. The anatomy of the knees and hips was well defined. The hip effusions were all easily recognized. MR images of the swine knees clearly demonstrated the experimentally produced joint effusions. The hip effusions and the experimentally introduced blood and saline all had long T1 and T2 values allowing easy differentiation from articular cartilage. We were unable to distinguish fresh blood from saline in the knee joints.
- Published
- 1986
- Full Text
- View/download PDF
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