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11 results on '"Notelaers, Tineke"'

1. MAPC culture conditions support the derivation of cells with nascent hypoblast features from bone marrow and blastocysts

Author

Lo Nigro, Antonio, Geraerts, Martine, Notelaers, Tineke, Roobrouck, Valerie D., Muijtjens, Manja, Eggermont, Kristel, Subramanian, Kartik, Ulloa-Montoya, Fernando, Park, Yonsil, Owens, Jason, Burns, Terry C., Low, Walter, Sharma, Shikha, Sohni, Abhishek, Crabbe, Annelies, Pauwelyn, Karen, Roelandt, Philip, Agirre, Xabier, Prosper, Felipe, OʼBrien, Timothy D., Zwijsen, An, Hu, Wei-Shou, Binas, Bert, and Verfaillie, Catherine M.

Published
2012
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2. Integrative and perturbation-based analysis of the transcriptional dynamics of TGFβ/BMP system components in transition from embryonic stem cells to neural progenitors

Author

Dries, Ruben, primary, Stryjewska, Agata, additional, Coddens, Kathleen, additional, Okawa, Satoshi, additional, Notelaers, Tineke, additional, Birkhoff, Judith, additional, Dekker, Mike, additional, Verfaillie, Catherine M., additional, Sol, Antonio, additional, Mulugeta, Eskeatnaf, additional, Conidi, Andrea, additional, Grosveld, Frank G., additional, and Huylebroeck, Danny, additional

Published
2019
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3. Integrative and perturbation-based analysis of the transcriptional dynamics of TGFβ/BMP system components in transition from embryonic stem cells to neural progenitors.

Author

Dries, Ruben, Stryjewska, Agata, Coddens, Kathleen, Satoshi Okawa, Notelaers, Tineke, Birkhoff, Judith, Dekker, Mike, Verfaillie, Catherine M., del Sol, Antonio, Mulugeta, Eskeatnaf, Conidi, Andrea, Grosveld, Frank G., and Huylebroeck, Danny

Subjects
GENETIC transcription, TRANSFORMING growth factors-beta, BONE morphogenetic proteins, EMBRYONIC stem cells, EMBRYOLOGY, MESSENGER RNA
Abstract

Cooperative actions of extrinsic signals and cell-intrinsic transcription factors alter gene regulatory networks enabling cells to respond appropriately to environmental cues. Signaling by transforming growth factor type β (TGFβ) family ligands (eg, bone morphogenetic proteins [BMPs] and Activin/Nodal) exerts cell-type specific and context-dependent transcriptional changes, thereby steering cellular transitions throughout embryogenesis. Little is known about coordinated regulation and transcriptional interplay of the TGFβ system. To understand intrafamily transcriptional regulation as part of this system's actions during development, we selected 95 of its components and investigated their mRNA-expression dynamics, gene-gene interactions, and single-cell expression heterogeneity in mouse embryonic stem cells transiting to neural progenitors. Interrogation at 24 hour intervals identified four types of temporal gene transcription profiles that capture all stages, that is, pluripotency, epiblast formation, and neural commitment. Then, between each stage we performed esiRNA-based perturbation of each individual component and documented the effect on steady-state mRNA levels of the remaining 94 components. This exposed an intricate system of multilevel regulation whereby the majority of gene-gene interactions display a marked cell-stage specific behavior. Furthermore, single-cell RNA-profiling at individual stages demonstrated the presence of detailed co-expression modules and subpopulations showing stable co-expression modules such as that of the core pluripotency genes at all stages. Our combinatorial experimental approach demonstrates how intrinsically complex transcriptional regulation within a given pathway is during cell fate/state transitions. [ABSTRACT FROM AUTHOR]

Published
2020
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5. SMAD signaling regulates CXCL12 expression in osteoblasts, affecting homing and mobilization of hematopietic progenitors

Author

Khurana, Satish, Melacarne, Alessia, Yadak, Rana, Schouteden, Sarah, Notelaers, Tineke, Pistoni, Elena, Maes, Christa, and Verfaillie, Catherine

Subjects
animal structures, embryonic structures, biological phenomena, cell phenomena, and immunity, biological factors
Abstract

We recently demonstrated that ex vivo activation of SMAD-independent bone morphogenetic protein 4 (BMP4) signaling in hematopoietic stem/progenitor cells (HSPCs) influences their homing into the bone marrow (BM). Here, we assessed whether alterations in BMP signaling in vivo affects adult hematopoiesis by affecting the BM niche. We demonstrate that systemic inhibition of SMAD-dependent BMP signaling by infusion of the BMP antagonist noggin (NGN) significantly increased CXCL12 levels in BM plasma leading to enhanced homing and engraftment of transplanted HSPCs. Conversely, the infusion of BMP7 but not BMP4, resulted in decreased HSPC homing. Using ST2 cells as an in vitro model of BM niche, we found that incubation with neutralizing anti-BMP4 antibodies, NGN, or dorsomorphin (DM) as well as knockdown of Smad1/5 and Bmp4, all enhanced CXCL12 production. Chromatin immunoprecipitation identified the SMAD-binding element in the CXCL12 promoter to which SMAD4 binds. When deleted, increased CXCL12 promoter activity was observed, and NGN or DM no longer affected Cxcl12 expression. Interestingly, BMP7 infusion resulted in mobilization of only short-term HSCs, likely because BMP7 affected CXCL12 expression only in osteoblasts but not in other niche components. Hence, we describe SMAD-dependent BMP signaling as a novel regulator of CXCL12 production in the BM niche, influencing HSPC homing, engraftment, and mobilization. ispartof: Stem Cells vol:32 issue:11 pages:3012-3022 ispartof: location:England status: published

Published
2014

8. 18F-FDG Labeling of Mesenchymal Stem Cells and Multipotent Adult Progenitor Cells for PET Imaging: Effects on Ultrastructure and Differentiation Capacity

Author

Wolfs, Esther, primary, Struys, Tom, additional, Notelaers, Tineke, additional, Roberts, Scott J., additional, Sohni, Abhishek, additional, Bormans, Guy, additional, Van Laere, Koen, additional, Luyten, Frank P., additional, Gheysens, Olivier, additional, Lambrichts, Ivo, additional, Verfaillie, Catherine M., additional, and Deroose, Christophe M., additional

Published
2013
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