1. Etiologic features of diarrheagenic microbes in stool specimens from patients with acute diarrhea in Thailand
- Author
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Supalert Nedsuwan, Siriporn Chantaroj, Sho Komukai, Piyada Wangroongsarb, Weerawat Manosuthi, Patpong Udompat, Warawan Wongboot, Namfon Suebwongsa, Watcharaporn Kamjumphol, Suwatthiya Kitsaran, Chotipong Siripipattanamongkol, Chareeya Thanee, Thanee Wongchai, Pipat Kluabwang, Pilailuk Akkapaiboon Okada, Charoen Jaiwong, Witaya Swaddiwudhipong, Lakkana Jirapong, Norrathep Assawapatchara, Shigeyuki Hamada, Patchanee Khum-on, Nuttagarn Chuenchom, and Kazuhisa Okada
- Subjects
Diarrhea ,Male ,0301 basic medicine ,Microbiological culture ,Viral epidemiology ,030106 microbiology ,lcsh:Medicine ,Real-Time Polymerase Chain Reaction ,medicine.disease_cause ,Article ,Microbiology ,Feces ,03 medical and health sciences ,fluids and secretions ,Rotavirus ,parasitic diseases ,Multiplex polymerase chain reaction ,medicine ,Humans ,Shigella ,lcsh:Science ,Clinical microbiology ,Infectious-disease epidemiology ,Multidisciplinary ,Bacteria ,business.industry ,Campylobacter ,lcsh:R ,Infectious-disease diagnostics ,Thailand ,Diarrhoea ,030104 developmental biology ,Acute Disease ,Etiology ,lcsh:Q ,Female ,medicine.symptom ,business ,Multiplex Polymerase Chain Reaction ,Asymptomatic carrier - Abstract
Many microbial species have been recognized as enteropathogens for humans. Here, we predicted the causative agents of acute diarrhea using data from multiplex quantitative PCR (qPCR) assays targeting 19 enteropathogens. For this, a case-control study was conducted at eight hospitals in Thailand. Stool samples and clinical data were collected from 370 hospitalized patients with acute diarrhea and 370 non-diarrheal controls. Multiple enteropathogens were detected in 75.7% and 13.0% of diarrheal stool samples using multiplex qPCR and bacterial culture methods, respectively. Asymptomatic carriers of enteropathogens were found among 87.8% and 45.7% of individuals by qPCR and culture methods, respectively. These results suggested the complexity of identifying causative agents of diarrhea. An analysis using the quantification cut-off values for clinical relevance drastically reduced pathogen-positive stool samples in control subjects from 87.8% to 0.5%, whereas 48.9% of the diarrheal stool samples were positive for any of the 11 pathogens. Among others, rotavirus, norovirus GII, Shigella/EIEC, and Campylobacter were strongly associated with acute diarrhea (P-value
- Published
- 2020
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