219 results on '"Noriyuki Tatsumi"'
Search Results
2. Guidelines for the evaluation of blood cell analysers including those used for differential leucocyte and reticulocyte counting and cell marker applications
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W. Groner, N. K. Shinton, S. M. Lewis, W.H. Coulter, R. Thom, K. Fujimoto, Brian S. Bull, R.M. Rowan, O.W. Assendelft, A.R. Jones, Christine E. McLaren, R.L. Verwilghen, John A. Koepke, Noriyuki Tatsumi, and J. M. England
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Cell marker ,medicine.medical_specialty ,Pathology ,Hematology ,Biology ,Blood cell ,medicine.anatomical_structure ,Reticulocyte ,Internal medicine ,Immunology ,medicine ,Cytometry ,Differential (mathematics) - Published
- 2008
3. [Untitled]
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NORIYUKI TATSUMI
- Published
- 2007
4. Reticulocyte Count Used to Assess Recombinant Human Erythropoietin Sensitivity in Hemodialysis Patients
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Kazuhide Kojima, Seiji Yamagami, Yasuzi Itoh, Izumi Tsuda, Hiroshi Tanaka, and Noriyuki Tatsumi
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business.industry ,Reticulocyte count ,law ,Erythropoietin ,medicine.medical_treatment ,Recombinant DNA ,Medicine ,Sensitivity (control systems) ,Hemodialysis ,Pharmacology ,business ,law.invention ,medicine.drug - Published
- 2015
5. Detection of Coxiella burnetii in Market Chicken Eggs and Mayonnaise
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Ying Qiao, Noriyuki Tatsumi, Kazuo Yamaguchi, Andreas Baumgartner, and Ikkyu Yamamoto
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biology ,Food Handling ,Eggs ,General Neuroscience ,Q fever ,Coxiella burnetii ,biology.organism_classification ,medicine.disease ,Polymerase Chain Reaction ,General Biochemistry, Genetics and Molecular Biology ,Microbiology ,Real-time polymerase chain reaction ,History and Philosophy of Science ,embryonic structures ,Food Microbiology ,medicine ,Food science ,Nested polymerase chain reaction - Abstract
We tried to detect C. burnetii in market chicken eggs and mayonnaise by nested PCR assay. The PCR target was the com 1 gene of C. burnetii. The positive rate for egg and mayonnaise samples was 4.2% and 17.6%, respectively. Direct sequence of some of the positive egg samples shows mutations whereas no mutation was found in the positive mayonnaise samples. The number of molecules of the Q fever agent is estimated at 10(4) to 10(6) per egg, according to our quantitative PCR test.
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- 2006
6. Body fat mass and lean mass as predictors of survival in hemodialysis patients
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Hidenori Koyama, Eiji Ishimura, Eiji Kimoto, Hideki Tahara, Y. Nishizawa, Kayo Shinohara, Sawako Hatsuda, Takami Miki, M. Emoto, Tsutomu Tabata, Yoshihiro Tsujimoto, Ryusuke Kakiya, Noriyuki Tatsumi, and Takuhito Shoji
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Adult ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,Population ,predictor ,Lower risk ,Protein-Energy Malnutrition ,Absorptiometry, Photon ,Predictive Value of Tests ,Renal Dialysis ,Risk Factors ,lean mass ,Internal medicine ,medicine ,Body Fat Distribution ,Humans ,education ,Dialysis ,Aged ,Proportional Hazards Models ,Retrospective Studies ,Univariate analysis ,education.field_of_study ,hemodialysis ,Proportional hazards model ,business.industry ,Middle Aged ,mortality ,body fat ,Endocrinology ,Adipose Tissue ,Nephrology ,Multivariate Analysis ,Body Composition ,Lean body mass ,Kidney Failure, Chronic ,Female ,Hemodialysis ,business ,Body mass index ,Follow-Up Studies - Abstract
A higher body mass index (BMI) is a predictor of better survival in hemodialysis patients, although the relative importance of body fat and lean mass has not been examined in the dialysis population. We performed an observational cohort study in 808 patients with end-stage renal disease on maintenance hemodialysis. At baseline, fat mass was measured by dual-energy X-ray absorptiometry and expressed as fat mass index (FMI; kg/m2). Lean mass index (LMI) was defined as BMI minus FMI. During the mean follow-up period of 53 months, 147 deaths, including 62 cardiovascular (CV) and 85 non-CV fatal events, were recorded. In univariate analysis, LMI was not significantly associated with CV or non-CV death, whereas a higher FMI was predictive of lower risk for non-CV death. Analyses with multivariate Cox models, which took other confounding variables as covariates, indicated the independent associations between a higher LMI and a lower risk of CV death, as well as between a higher FMI and a lower risk of non-CV death. These results indicate that increased fat mass and lean mass were both conditions associated with better outcomes in the dialysis population.
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- 2006
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7. Efficacy and Applicability of Astrim, a Portable Non-invasive Device Evaluating Hemoglobin and Peripheral Circulation
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Noriyuki Tatsumi and Tamaki Matsumoto
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medicine.medical_specialty ,business.industry ,Non invasive ,Medicine ,Medical physics ,Hemoglobin levels ,business ,Medical research ,Near infrared radiation ,Biomedical engineering - Abstract
A widely used non-invasive measurement employing nearinfrared radiation provides valuable information in the clinical and basic medical research field. The Astrim, a portable non-invasive monitoring device recently developed, evaluates peripheral circulation as well as hemoglobin levels. The present study conducted several examinations to scrutinize the reproducibility of results, efficacy, and validity of the device. Our data demonstrated the usefulness of the non-invasive monitoring device in diverse clinical circumstances and its applicability to basic physiological and medical research fields.
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- 2006
8. [Untitled]
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NORIYUKI TATSUMI
- Published
- 2006
9. Autonomic nervous system activity in the late luteal phase of eumenorrheic women with premenstrual symptomatology
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Mina Morimura, Takashi Suzuki, Tatsuya Hayashi, Tamaki Matsumoto, Noriyuki Tatsumi, Toshio Moritani, and Takahisa Ushiroyama
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Adult ,medicine.medical_specialty ,Sympathetic nervous system ,Rest ,media_common.quotation_subject ,Luteal Phase ,Luteal phase ,Autonomic Nervous System ,Severity of Illness Index ,Premenstrual Syndrome ,Electrocardiography ,Parasympathetic nervous system ,Heart Rate ,Parasympathetic Nervous System ,Surveys and Questionnaires ,Internal medicine ,Heart rate ,Follicular phase ,medicine ,Humans ,Heart rate variability ,Menstrual Cycle ,Menstrual cycle ,media_common ,Analysis of Variance ,Obstetrics and Gynecology ,Psychiatry and Mental health ,Clinical Psychology ,Autonomic nervous system ,medicine.anatomical_structure ,Endocrinology ,Follicular Phase ,Reproductive Medicine ,Female ,Psychology - Abstract
The majority of women of reproductive age experience a regular recurrence of various symptoms in the premenstrual phase. The etiopathogenesis of premenstrual symptomatology, however, remains inconclusive. The present study was proposed to evaluate whether the activity of the autonomic nervous system (ANS), which largely contributes to the relative stability of a human's internal environment, is altered during the menstrual cycle of women with premenstrual symptomatology. Thirty eumenorrheic young women participated in this study. All subjects were investigated during the follicular and late luteal phases. The ANS activity was assessed by means of heart rate variability power spectral analysis during supine rest. No intramenstrual cycle differences in the ANS activity were found in women experiencing no or small increases in premenstrual symptoms. In contrast, the sympathetic nervous system (SNS) activity significantly increased and the parasympathetic nervous system (PNS) activity apparently decreased in the late luteal phase in subjects whose premenstrual symptomatology was not unbearable, but substantially increased (> 20%) compared to the symptom-free follicular phase. The women with greater degrees of premenstrual distress possessed higher SNS activity and lower PNS activity in the late luteal phase than the women with less symptomatology. The ANS activity in the follicular phase did not differ among the subjects regardless of their premenstrual symptoms. Although causes and consequences continue to elude, the present study provides additional intriguing evidence that the altered functioning of ANS in the late luteal phase could be associated with diverse psychosomatic or behavioral symptoms appearing premenstrually.
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- 2006
10. Improved Survival in Patients with Multiple Myeloma Treated with DMVM plus IFN-.ALPHA
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Takashi Kageyama, Yoshiteru Konaka, Yukihiro Tokumine, Atsushi Horiuchi, Tadahiro Tsubakio, Hedeki Fujitake, Noriyuki Tatsumi, Kiyoyasu Nagai, Tohru Masaoka, Teruo Kitani, J. Kuyama, Machiko Tsukaguchi, Yonezawa T, Kunio Hayashi, Kaori Nasu, Hiroya Kawagoe, and Eizo Kakishita
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Melphalan ,medicine.medical_specialty ,Vincristine ,business.industry ,Improved survival ,Combination chemotherapy ,General Medicine ,medicine.disease ,Gastroenterology ,Surgery ,Regimen ,Internal medicine ,medicine ,In patient ,business ,Multiple myeloma ,Dexamethasone ,medicine.drug - Abstract
We examined effects of combination chemotherapy with dexamethasone, melphalan, vincristine, and MCNU (DMVM), plus IFN-α in patients with previously untreated and treated multiple myeloma (MM). In the study, 78 previously untreated and 47 treated MM patients were evaluated. The overall response rate was 76% [27% complete response (CR)] : 85% [37% CR] in previously untreated patients and 62% [11% CR] in previously treated patients. The 50% survival time was 45.3 months for untreated patients and 30.1 months for previously treated patients. This regimen is effective in producing a high CR rate and prolonging survival duration of MM patients.
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- 2005
11. Validation of Platelet Counting Accuracy With the Celltac F Automated Hematology Analyzer
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Noriyuki Tatsumi, Hiroshi Kondo, and Yutaka Nagai
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Pathology ,medicine.medical_specialty ,Chromatography ,business.industry ,Becton dickinson ,Computer Science Applications ,Analytical Chemistry ,Hematology analyzer ,Dilution ratio ,Platelet counting ,Chemical Engineering (miscellaneous) ,Medicine ,Vacutainer ,business ,Instrumentation ,Research Article - Abstract
Rapid and accurate analysis of platelet count plays an important role in evaluating hemorrhagic status. Therefore, we evaluated platelet counting performance of a hematology analyzer, Celltac F (MEK-8222, Nihon Kohden Corporation, Tokyo, Japan), that features easy use with low reagent consumption and high throughput while occupying minimal space in the clinical laboratory. All blood samples were anticoagulated with dipotassium ethylenediaminetetraacetic acid (EDTA-2K). The samples were stored at room temperature (18∘C–22∘C) and tested within 4 hours of phlebotomy. We evaluated the counting ability of the Celltac F hematology analyzer by comparing it with the platelet counts obtained by the flow cytometry method that ISLH and ICSH recommended, and also the manual visual method by Unopette (Becton Dickinson Vacutainer Systems). The ICSH/ISLH reference method is based on the fact that platelets can be stained with monoclonal antibodies to CD41 and/or CD61. The dilution ratio was optimized after the precision, coincidence events, and debris counts were confirmed by the reference method. Good correlation of platelet count between the Celltac F and the ICSH/ISLH reference method (r=0.99), and the manual visual method (r=0.93) were obtained. The regressions werey=0.90x+9.0andy=1.11x+8.4, respectively. We conclude that the Celltac F hematology analyzer for platelet counting was well suited to the ICSH/ISLH reference method for rapidness and reliability.
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- 2005
12. Anemia Screening Using a Compact Hemoglobin Meter
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Tamaki Matsumoto, Noriyuki Tatsumi, and Masaharu Yokota
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medicine.medical_specialty ,business.industry ,Anemia ,Physical therapy ,Medicine ,Hemoglobin ,Disease ,business ,medicine.disease ,Intensive care medicine ,Hemoglobinometry ,World health ,Anemia screening - Abstract
Anemia is a basic disease associated with various serious pathological conditions. Thus, effectively screening or monitoring patients for anemia is a critical issue for the World Health Organization. The recently developed Astrim SU, is a portable, noninvasive monitoring device for hemoglobin. In the present study, we investigated the efficacy and validity of the device for measuring basic items including hemoglobin and screening for anemia. Our data demonstrated the usefulness and applicability of the device in diverse clinical circumstances.
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- 2005
13. Comparison of haemoglobinometry by WHO Haemoglobin Colour Scale and copper sulphate against haemiglobincyanide reference method
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I. S. Timan, E. Wangsasaputra, Aulia D, and Noriyuki Tatsumi
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Male ,Pediatrics ,medicine.medical_specialty ,Copper Sulfate ,Clinical Biochemistry ,Color ,World Health Organization ,Sensitivity and Specificity ,Hemoglobins ,Primary health ,Humans ,Medicine ,In patient ,Screening tool ,Developing Countries ,Methemoglobin ,Blood donor screening ,business.industry ,Biochemistry (medical) ,Anemia ,Hematology ,General Medicine ,Reference Standards ,Surgery ,Hemoglobinometry ,Female ,business ,Anaemia screening - Abstract
Summary Although estimation of haemoglobin is essential for diagnosing anaemia and assessing its severity, many health centres in developing countries do not have the facilities for haemoglobinometry. The WHO Haemoglobin Colour Scale (HCS) method is a simple and inexpensive clinical device that was recently developed in order to diagnose anaemia in such centres. In Indonesia, the copper sulphate specific gravity method is used for blood donor screening and also in primary health clinics in the rural and remote areas. In this study, the HCS method is compared with the copper sulphate method and with an earlier paper scale, the Tallquist method, against the standard haemiglobincyanide spectrophotometric method. The HCS method showed an acceptable level of precision and accuracy for use as a reliable screening tool to diagnose anaemia in patients and also for blood donor screening.
- Published
- 2004
14. A Trial for Accuracy Improvement on Fibrinogen Assay
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Hiroshi Kondo, Noriyuki Tatsumi, Masaharu Yokota, and Masahiro Okuda
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Chromatography ,Fibrinogen assay ,business.industry ,Medicine ,business ,Accuracy improvement ,Fibrinogen ,medicine.drug - Published
- 2004
15. XVIIth International Symposium on Technological Innovations in Laboratory Hematology
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Margarida Lima, Rhonda Becker, Josep M Jou, G Gutierrez, JC Reverter, Kap No Lee, Soo-Young Yoon, Han-Ik Cho, Noriyuki Tatsumi, Tomas Maramba, Elkin Simson, Jesus Villarrubia, Szu-Hee Lee, Linda M. Sandhaus, Lothar Thomas, G�nter Valet, Wolf-Dieter Ludwig, M. Tarek Elghetany, Alberto Orfao, John D. Olson, Samuel J Machin, Kandice Kottke-Marchant, Steve Kitchen, Frank J.T. Staal, John Goldman, Claire Harrison, and Carlo L. Balduini
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Laboratory.hematology ,Engineering ,business.industry ,Biochemistry (medical) ,Clinical Biochemistry ,Physiology ,Library science ,Hematology ,business - Published
- 2004
16. Preparation of a purified fibrinogen calibration material for Clauss method and turbidimetric immunoassay possessing biological activity and antigenicity
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K. Naka, Masahiro Okuda, Noriyuki Tatsumi, and Y. Uemura
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Prothrombin time ,Chromatography ,medicine.diagnostic_test ,Chemistry ,FIBRINOGEN STANDARD ,Hematology ,Thrombin time ,Fibrinogen ,Fibrinogen Measurement ,Immunoassay ,Immunology ,Coagulation testing ,medicine ,Partial thromboplastin time ,medicine.drug - Abstract
Fibrinogen plays a major role in basic coagulation tests such as prothrombin time (PT), activated partial thromboplastin time (APTT) and thrombin time (TT). These show high interlaboratory variation because of inaccurate instrumental calibration. The same is true of the fibrinogen assay, despite its being a quantitative assay. Most medical laboratories use automated coagulometers and commercially available calibration materials (calibrators) to obtain an accurate fibrinogen value, but, when checked, calibrators have been found to deviate from the assigned value. The Japan Society of Laboratory Medicine (JSLM) has identified the need for a reliable plasma fibrinogen standard. To enhance the reliability of calibrator fibrinogen values and thereby remedy the poor precision and accuracy of plasma fibrinogen testing, we undertook the preparation of a standard calibration material applicable to both the Clauss method and turbidimetric immunoassay (TIA). The calibrator was prepared from fresh human plasma by glycine precipitation and virus inactivation followed by affinity chromatography to remove contaminated plasminogen. In the resulting product, clottable fibrinogen accounted for 95% of total protein and within-run precision showed a CV of less than 1.8%. We believe the preparation will become a candidate material for laboratory and manufacturer use in Japan.
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- 2003
17. Electrocardiogram is very useful for predicting acute heart failure following myeloablative chemotherapy with hematopoietic stem cell transplantation rescue
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Takahisa Yamane, Takehiro Hayashi, Hirohisa Nakamae, Mika Akahori, Kensuke Ohta, Kei Tsumura, Seiichi Kitagawa, Noriyuki Tatsumi, and Masayuki Hino
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Adult ,Male ,medicine.medical_specialty ,Heart disease ,Systole ,medicine.medical_treatment ,Diastole ,Hematopoietic stem cell transplantation ,QT interval ,Electrocardiography ,Predictive Value of Tests ,Risk Factors ,Internal medicine ,Humans ,Medicine ,Prospective Studies ,cardiovascular diseases ,Prospective cohort study ,Heart Failure ,Transplantation ,medicine.diagnostic_test ,business.industry ,Hematopoietic Stem Cell Transplantation ,Hematology ,Middle Aged ,Myeloablative Agonists ,medicine.disease ,Confidence interval ,Surgery ,Long QT Syndrome ,Heart failure ,Acute Disease ,Multivariate Analysis ,Cardiology ,Female ,business ,Biomarkers - Abstract
A prospective study was conducted in 71 evaluable patients who received myeloablative hematopoietic stem cell transplantation (HSCT) at our facility from 1995 to 2002, to find a sensitive marker for post-transplant heart failure, including echocardiographic systolic and diastolic markers and QTc interval. QTc was found to be an independent and significant risk factor for acute heart failure (AHF) on multivariate logistic regression analysis (OR 1.5, P=0.01, 95% confidence interval (CI) 1.1-2.0), while no significant differences between patients with AHF and those without AHF were found in age, sex, treatment history, type of conditioning regimen, and echocardiographic systolic and diastolic markers. On further analysis, post-transplant risk of AHF appeared to be increased as QTc was prolonged. The post-transplant risk of AHF in the group with longest QTc on multivariate logistic regression analysis was found to be 9.8 times that in the group with shortest QTc (P=0.04, 95% CI 1.0-100). These results suggest that echocardiographic markers are less valuable predictors of post-transplant AHF, but that prolongation of the QTc, an ECG marker, before HSCT is strongly associated with onset of AHF after HSCT.
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- 2003
18. A New Method of Purifying Fibrinogen with Both Biological and Immunological Activity from Human Plasma
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Noriyuki Tatsumi, Masahiro Okuda, and Yahiro Uemura
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Quality Control ,Sodium ,chemistry.chemical_element ,Fractionation ,Chemical Fractionation ,Fibrinogen ,Biochemistry ,Chromatography, Affinity ,Fibrinogen Measurement ,Plasma ,Affinity chromatography ,medicine ,Chemical Precipitation ,Humans ,Polyacrylamide gel electrophoresis ,Chromatography ,Chemistry ,Reproducibility of Results ,General Medicine ,Urokinase-Type Plasminogen Activator ,Electrophoresis ,Coagulation ,Virus Inactivation ,Electrophoresis, Polyacrylamide Gel ,Algorithms ,Biotechnology ,medicine.drug - Abstract
Using a combination of Cohn ethanol fractionation, virus inactivation, glycine and sodium chloride precipitation, and lysine-Sepharose affinity chromatography, a unique and rapid simplified method was developed to obtain highly purified fibrinogen for diagnostic use with both biological (Clauss method) and immunological (Jacobsson method) activity. Yield was 0.66 g of fibrinogen per liter of starting pooled plasma, and the purified product showed good agreement in activity with the starting material. The purified fibrinogen solution contained over 95% clottable protein and had a clear appearance. No degradation was observed after urokinase treatment and the preparation provided good precision in fibrinogen measurement compared to pooled plasma. The simplified method was, thus, shown to result in a high-purity fibrinogen preparation, suitable for in vitro diagnostic use, as well as for use to prepare a fibrinogen reference material and to perform fibrinogen quality control using an automated coagulation analyzer.
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- 2003
19. Diagnostic Usefulness of Erythrocyte Sedimentation Rate Assay as a Marker of Chronic Disorders in the Health Evaluation and Promotion Schema
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Toshiyuki Akiyama, Tomomi Tabuchi, Masaharu Yokota, Noriyuki Tatsumi, and Hiroshi Kondo
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medicine.medical_specialty ,medicine.diagnostic_test ,Health evaluation ,business.industry ,Internal medicine ,Schema (psychology) ,Erythrocyte sedimentation rate ,Immunology ,medicine ,business ,Chronic disorders - Abstract
急激な高齢化の進展を迎えている我が国では, 生活習慣病の予防が社会的関心を集め, 諸種の臨床化学および血液学的検査が疾病の早期発見, 予防, 病態把握のため1ご利用されている。疾病のスクリーニングには, 容易で迅速に実施できる自動分析法が繁用されているものの, 赤血球沈速度 (赤沈) は自動分析になしまないことから健診の場てはあまり利用されていない現状にある。また, 赤沈はCRPと同様の臨床的意義しかないとの誤解もある。このことから赤沈測定の診断的有用性と, そのCRPとの違いについて最近の知見を本稿でまとめてみた。
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- 2003
20. [Untitled]
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NORIYUKI TATSUMI
- Published
- 2003
21. Quality control material for plasma fibrinogen test produced from purified human fibrinogen
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Yahiro Uemura, Masahiro Okuda, and Noriyuki Tatsumi
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Chromatography ,business.industry ,Coefficient of variation ,Fibrinogen ,Blood proteins ,Human fibrinogen ,Computer Science Applications ,Analytical Chemistry ,Fibrinogen Measurement ,Coagulation ,Reagent ,Immunology ,medicine ,Chemical Engineering (miscellaneous) ,Control material ,business ,Instrumentation ,Research Article ,medicine.drug - Abstract
Plasma fibrinogen measurement is a routine laboratory procedure commonly performed on automated coagulation analysers. Its determination is quantitative, not quantitative. Yet, a lack of precision has been an issue for fibrinogen measurement. A control material derived from plasma comprises many proteins, inhibitors and fatty acids, any or all of which can interfere in the fibrinogen assay. This study has attempted to develop a quality control material using purified human fibrinogen and has compared measurement precision between both purified and plasma materials. Purified fibrinogen was prepared using Cohn fraction 1 and glycine precipitation. Purified fibrinogen clottability was greater than 95%, with no main plasma proteins, lipids or fibrinogen degradation products observed. Two purified control materials were lyophilized at normal (2.30 g lm1) and abnormal (1.20 g lm1) levels of fibrinogen concentration. Precision was evaluated using a liquid-type reagent, Thrombocheck Fib(L), on automated coagulation analysers. Coefficient of variation for within-run, intraday and between-day precision of the purified materials was 0.7-3.5%. In comparison, the coefficient of variation for plasma materials ranged from 1.2 to 5.3%. These results suggest that materials prepared from purified fibrinogen can be useful to laboratory quality control by improving overall precision of fibrinogen measurement and are applicable to automated coagulation analysers.
- Published
- 2003
22. Availability of Danaparoid, an Anticoagulant, for Routine Hematological Tests
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Noriyuki Tatsumi, Takuya Nishioka, Hiroshi Kondo, and Masaharu Yokota
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medicine.medical_specialty ,Hematology ,KANAMYCIN SULFATE ,Platelet aggregation ,medicine.drug_class ,Chemistry ,Anticoagulant ,Danaparoid ,Heparin ,medicine.disease ,Internal medicine ,Anesthesia ,medicine ,Coagulopathy ,Platelet ,Intensive care medicine ,medicine.drug - Abstract
In the Multiphasic Health Check-up System, medical laboratory testing has been changing lately to utilize a small and compact type of testing systems. The system uses heparinized blood for chemistry tests to reduce laboratory's workload and sample volume. The dana-paroid, a mixture of glycosamino-glycans (Heparan sulfate), has been used clinically for treatment of patients with disseminated intravascular coagulopathy instead of heparin sulfate. Heparin has been used for routine laboratory tests, although it has a strong activity of platelet aggregation. This study aimed to examine availability of Danaparoid for routine hematology tests as a substitute of heparin. Although Danaparoid induced platelet aggregation directly, the reaction was weaker than that of heparin. The platelet count of Danaparoid blood was decreased after five minutes of mixing. Addition of MgSO4 or of kanamycin sulfate to Danaparoid blood was able to prevent this decrease. When Danaparoid blood was applied to chemistry tests, major chemistry test data showed acceptable results, although level differences were observed slightly. It was concluded that Danaparoid would be a good candidate for a universal anticoagulant for both chemical and hematological laboratory tests.
- Published
- 2003
23. Differential effects of fibroblast growth factor-4, epidermal growth factor and transforming growth factor-β1 on functional development of stromal layers in acute myeloid leukemia
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Noriyuki Tatsumi, Takahisa Yamane, Kensuke Ohta, Hirohisa Nakamae, Ki-Ryang Koh, Masayuki Hino, and Takayuki Takubo
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Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Stromal cell ,medicine.medical_treatment ,Cell Culture Techniques ,Fibroblast Growth Factor 4 ,CD34 ,Cell Count ,Cell Communication ,Biology ,Fibroblast growth factor ,Transforming Growth Factor beta1 ,Bone Marrow ,Transforming Growth Factor beta ,Epidermal growth factor ,Proto-Oncogene Proteins ,Internal medicine ,medicine ,Humans ,Progenitor cell ,Growth Substances ,Aged ,Aged, 80 and over ,Epidermal Growth Factor ,Growth factor ,Hematology ,Middle Aged ,Hematopoietic Stem Cells ,Coculture Techniques ,Fibroblast Growth Factors ,Cytokine ,Endocrinology ,Oncology ,Leukemia, Myeloid ,Acute Disease ,Cancer research ,Female ,Stromal Cells ,Cell Division ,Transforming growth factor - Abstract
The hematopoietic supporting abilities are known to be impaired in marrow stromal layers developed from patients with acute myeloid leukemia (AML). In this study, fibroblast growth factor-4 (FGF-4), epidermal growth factor (EGF) or transforming growth factor-beta1 (TGF-beta1) were studied to see whether these growth factors can modify the functional development of leukemic stromal layers. Adherent stromal layers from 13 patients with AML and from six non-leukemic controls were established with 3ng/ml of FGF-4, EGF or TGF-beta1. Established stromal layers were washed three times and irradiated, followed by recharge of allogenic peripheral CD34 positive cells as an indicator of supportive function. Progenitor-outputs into supernatant were evaluated at biweekly interval with colony-forming assay until 6 weeks. The results showed that both leukemic and non-leukemic stromal cells established with FGF-4, but not with EGF, showed significantly higher progenitor cell-outputs compared with control stromal cells. By contrast, stromal cells developed with TGF-beta1 showed significantly lower progenitor cell-outputs compared with control. These differences were significant at later than 4 weeks after the recharge of indicator cells, suggesting that the stromal layer developed with EGF or TGF-beta1 preferentially affected the primitive progenitors rather than committed ones. These results indicate that FGF-4 and TGF-beta1 differentially affect the functional development of leukemic as well as of normal stromal layers.
- Published
- 2002
24. Role of mitogen-activated protein kinase family in serum-induced leukaemia inhibitory factor and interleukin-6 secretion by bone marrow stromal cells
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Hiroshi Iwao, Masayuki Hino, Shokei Kim, Katsuyuki Miura, Takafumi Nakao, Kensuke Ohta, Hitomi Kawano, and Noriyuki Tatsumi
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Pharmacology ,MAPK/ERK pathway ,Stromal cell ,medicine.medical_treatment ,Biology ,Cell biology ,Haematopoiesis ,Cytokine ,medicine.anatomical_structure ,Cancer research ,medicine ,Cytokine secretion ,Bone marrow ,Protein kinase A ,Leukemia inhibitory factor - Abstract
In the haematopoietic microenvironment, bone marrow stromal cells play an important role in regulating haematopoiesis by expressing various cytokines, including leukaemia inhibitory factor (LIF) and interleukin-6 (IL-6). However, the intracellular signal that regulates cytokine secretion in bone marrow stromal cells has not been determined. The aim of this study was to evaluate the role of mitogen-activated protein kinase (MAPK) family in serum-induced secretion of LIF and IL-6 by bone marrow stromal cells. Transformed human bone marrow stromal cells (HS-5) were stimulated with foetal calf serum (FCS) to produce LIF and IL-6. FCS also induced activation of extracellular signal-regulated kinase (ERK), p38 MAPK and c-Jun NH2-terminal kinase (JNK). Both PD98059 (MAPK/ERK kinase inhibitor) and SB203580 (p38 MAPK inhibitor) attenuated FCS-induced LIF protein production and gene expression. SB203580 decreased IL-6 production and gene expression, but PD98059 had no effect on IL-6 production and gene expression. Expression of a dominant-negative mutant form of JNK1 that blocked FCS-induced JNK activity had no effect on protein production and gene expression of these cytokines. These findings demonstrate that both ERK and p38 MAPK are involved in FCS-induced LIF secretion, whereas only p38 MAPK is important for IL-6 secretion, and that FCS-induced activation of JNK has no effect on the production of LIF and IL-6. We conclude that, in spite of their similar biological effects, they are differentially regulated at the level of MAPK activity in bone marrow stromal cells. British Journal of Pharmacology (2002) 136, 975–984. doi:10.1038/sj.bjp.0704797
- Published
- 2002
25. Simple high-density lipoprotein cholesterol assay based on dry chemistry
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Seiichi Kitagawa, Tetsuaki Yamada, Noriyuki Tatsumi, Masayuki Hino, Susumu Nishino, and Takayuki Takubo
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Time Factors ,Point-of-Care Systems ,Clinical Biochemistry ,Sensitivity and Specificity ,Biochemistry ,Surface-Active Agents ,chemistry.chemical_compound ,Pulmonary surfactant ,Humans ,Whole blood ,Chromatography ,Chemistry ,Cholesterol ,Cholesterol, HDL ,Biochemistry (medical) ,Reproducibility of Results ,General Medicine ,Ascorbic acid ,Dilution ,Logistic Models ,Spectrophotometry ,Reagent ,Hemoglobin ,Quantitative analysis (chemistry) ,Blood Chemical Analysis - Abstract
Background: Currently, high-density lipoprotein cholesterol (HDL-C), a factor which prevents progression of arteriosclerosis, is measured using laboratory-based chemistry analyzers without a pretreatment step. Because HDL-C is measured with a pretreatment step in many point-of-care testing systems, a direct assay is needed. Methods: A dry-chemistry-based assay using surfactants has recently been developed in parallel with the development of a dedicated reagent. A simple analyzer that accepts whole blood samples was also developed. Results: The assay demonstrated excellent precision, dilution linearity and intermethod comparison. In an interference test, assay values tended to be lower in the presence of high concentrations of hemoglobin, conjugated or unconjugated bilirubin. Neither ascorbic acid up to 20 mg/dl, nor formazin turbidity up to 2100, had an effect on the assay. Conclusions: This dry-chemistry assay using only surfactants for specificity in the direct HDL-C method was judged useful for point-of-care instrumentation in terms of equipment compactness, operational simplicity and rapid responsiveness.
- Published
- 2002
26. The HLA-DOB gene displays limited polymorphism with only one amino acid substitution
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K. Yamano, Noriyuki Tatsumi, H. Kawata, Taeko Naruse, M. Hino, Hidetoshi Inoko, and Gen Isshiki
- Subjects
Genetics ,Linkage disequilibrium ,Immunology ,Single-nucleotide polymorphism ,General Medicine ,Human leukocyte antigen ,Biology ,Biochemistry ,DNA sequencing ,Polymorphism (computer science) ,Immunology and Allergy ,Allele ,Gene ,Peptide sequence - Abstract
The HLA-DO molecule is a non-classical class II heterodimer composed of alpha and beta chains. We have previously recognized that all eight of the allelic variations of the HLA-DOA gene represent non-synonymous amino acid substitution. In the present study, to analyze genetic polymorphism and allelic variation of the HLA-DOB gene which may affect the efficiency of class II restricted antigen presentation thereby being involved in the susceptibility of HLA associated diseases, we conducted direct DNA sequencing of HLA-DOB in 36 HLA class II homozygous typing cells and identified six new allelic variations (DOB*0101101, *0101102, *01012, *01022, *0104101 and *0104102) including five single nucleotide polymorphisms with only one amino acid substitution. Furthermore, strong linkage disequilibrium was detected between DOB*01022 and DRB1*1502 only, with no linkage disequilibrium between the DOA and the DOB genes. The HLA-DOB gene has been identified in other mammals, and their nucleotide sequences are well conserved. These facts suggest that limited polymorphism in the DOB gene is profitable to execute their unique function as a co chaperone and so strong selective pressure is operating to prevent generic variation against the DOB molecule interacting with the DM molecule and thus maintaining the specified immunological function of regulating antigen presentation.
- Published
- 2002
27. Flow cytometric analysis of platelet activation under calcium ion-chelating conditions
- Author
-
Noriyuki Tatsumi, Izumi Tsuda, Takuya Nishioka, and Masaharu Yokota
- Subjects
medicine.medical_specialty ,Chemistry ,Platelet Distribution Width ,Ethylenediaminetetraacetic acid ,Hematology ,chemistry.chemical_compound ,Endocrinology ,Biochemistry ,Pseudothrombocytopenia ,Internal medicine ,Sodium citrate ,medicine ,Platelet ,Platelet activation ,Mean platelet volume ,Whole blood - Abstract
Platelet activation and aggregation results in factitious counting and sizing in routine haematology testing. In this study, the possibility of platelet activation in anticoagulated solutions was examined. Whole blood was examined using an automated counter and a flow cytometer before and after strong vortex agitation. Blood treated with ethylenediaminetetraacetic acid (EDTA) exhibited platelet activation both pre- and postagitation but activated platelets did not cause platelet aggregation. With sodium citrate, platelets were only minimally activated both pre- and postagitation. Heparin-treated blood exhibited minimal platelet activation preagitation, but agitation resulted in strong platelet activation and aggregation. Platelet size was increased by agitation in blood with EDTA and with sodium citrate, in association with significant increases in mean platelet volume (MPV) and platelet distribution width (PDW), but MPV and PDW were significantly higher in EDTA solution than in sodium citrate solution. Change in platelet size was observed even in the presence of EDTA, indicating that careful sampling and processing are needed in the collection of specimens. Specimens obtained from patients with EDTA-dependent pseudothrombocytopenia exhibited the same level of activation as controls, although platelets exhibited aggregation in such specimens. In conclusion, platelet activation involving platelet size change can occur in the absence of calcium ions in blood treated with EDTA.
- Published
- 2002
28. A novel cell surface antigen, 4C8, is expressed on human eosinophils
- Author
-
Takahisa Yamane, Jun-Ichi Masuyama, Yasutaka Aoyama, Kensuke Ohta, Seiichi Kitagawa, Kenichi Suzuki, Noriyuki Tatsumi, and Masayuki Hino
- Subjects
Neutrophils ,medicine.drug_class ,Blotting, Western ,Biophysics ,Monoclonal antibody ,Peripheral blood mononuclear cell ,Monocytes ,Immunophenotyping ,Pathology and Forensic Medicine ,Flow cytometry ,Endocrinology ,Antigen ,medicine ,Humans ,Lymphocytes ,biology ,medicine.diagnostic_test ,Chemistry ,Receptors, IgG ,Antibodies, Monoclonal ,Cell Biology ,Hematology ,Eosinophil ,Flow Cytometry ,Molecular biology ,Eosinophils ,Blot ,medicine.anatomical_structure ,Antigens, Surface ,Immunology ,biology.protein ,Antibody ,Cytometry - Abstract
Background: A novel monoclonal antibody, anti-4C8, reacted with human peripheral lymphocytes and monocytes but not with neutrophils. In this study, we investigated whether the 4C8 antigen is expressed on human peripheral eosinophils. Methods: Expression of the 4C8 antigen on eosinophils was analyzed by flow cytometry and molecular analysis of the antigen was performed with eosinophils by Western blotting. Results: Among human peripheral granulocytes, the 4C8 antigen was expressed on CD16-negative cells but not on CD16-positive cells. The 4C8 antigen also appeared to be expressed on eosinophils. To confirm the latter finding, eosinophils were purified from peripheral blood. On flow cytometric analysis, anti-4C8 antibody reacted with purified eosinophils. On Western blotting analysis, anti-4C8 reacted with a single band of 80 kDa in lysates from purified eosinophils. The correlation between the percentage of eosinophils determined by May-Giemsa staining and the percentage of 4C8-positive/CD16-negative cells among granulocytes was good (r = 0.91, P < 0.0001). Conclusions: Only a few cell surface antigens are available to distinguish human peripheral eosinophils from neutrophils. The novel cell surface antigen, 4C8, is a useful new marker of human eosinophils. Cytometry (Clin. Cytometry) 50:8–13, 2002. © 2002 Wiley-Liss, Inc.
- Published
- 2002
29. New compact-type latex photometric immunoassay system for hemoglobin and three acute inflammation markers: Neutrophil count, C-reactive protein, and anti-streptolysin O
- Author
-
Masayuki Hino, Takahisa Yamane, Hironobu Ohta, Tatsuo Fukumori, Akio Okubo, Noriyuki Tatsumi, and Kensuke Ohta
- Subjects
Microbiology (medical) ,medicine.medical_specialty ,Neutrophils ,Clinical Biochemistry ,Sensitivity and Specificity ,Gastroenterology ,Hemoglobins ,Leukocyte Count ,Bacterial Proteins ,White blood cell ,Internal medicine ,Humans ,Immunology and Allergy ,Medicine ,Whole blood ,Immunoassay ,Inflammation ,biology ,medicine.diagnostic_test ,Diagnostic Tests, Routine ,business.industry ,Biochemistry (medical) ,C-reactive protein ,Public Health, Environmental and Occupational Health ,Reproducibility of Results ,Original Articles ,Hematology ,Antibodies, Bacterial ,Medical Laboratory Technology ,C-Reactive Protein ,medicine.anatomical_structure ,Streptolysins ,Immunology ,Absolute neutrophil count ,biology.protein ,Streptolysin ,Hemoglobin ,Anti-streptolysin O ,business ,Biomarkers ,Latex Fixation Tests - Abstract
A new compact‐type latex photometric immunoassay system, SPOTCHEM IM SI‐3510 (ARKRAY, Inc., Kyoto, Japan), which assays three kinds of inflammatory markers—neutrophil count (NPC), C‐reactive protein (CRP), and anti‐streptolysin O (ASO)—was evaluated. Hemoglobin (Hb), which is a good marker for anemia, can also be measured with it. NPC and CRP are measured using antibodies against neutrophilic elastase and CRP, purified streptolysin O was used for ASO determination, and Hb was measured by an azide‐methemoglobin method. Whole blood, serum, and plasma specimens can be used as samples with this system. In this study, whole blood treated with dipotassium ethylenediamine tetraacetic acid was used for evaluation. Linearity and reproducibility were good for all of the items studied. Good correlations were observed between the results obtained by this system and those obtained by routine methods. Since NPC exhibited a high correlation with the routine white blood cell (WBC) counts, it was judged to be useful as a substitute for WBC counting. Since this system is small and easy to operate, and evaluation revealed reliable results, it was judged to be practical for small laboratories, and satellite testing in hospitals and physicians’ office laboratories for patients suspected to have acute inflammation. J. Clin. Lab. Anal. 16:95–102, 2002. © 2002 Wiley‐Liss, Inc.
- Published
- 2002
30. Universal Anticoagulantsfor Medical Laboratory use
- Author
-
Noriyuki Tatsumi
- Subjects
medicine.medical_specialty ,business.industry ,Medical laboratory ,Medicine ,Medical physics ,business - Published
- 2002
31. Possible automatic cell classification of bone marrow aspirate using the CELL-DYN 4000®automatic blood cell analyzer
- Author
-
Izumi Tsuda, Noriyuki Tatsumi, Ryousuke Yamamura, Kensuke Ohta, Takahisa Yamane, Masayuki Hino, and Hisako Shibata
- Subjects
Microbiology (medical) ,medicine.medical_specialty ,Pathology ,Myeloid ,Hematology ,medicine.diagnostic_test ,business.industry ,Biochemistry (medical) ,Clinical Biochemistry ,Public Health, Environmental and Occupational Health ,medicine.disease ,Flow cytometry ,Bone marrow examination ,Blood cell ,Medical Laboratory Technology ,Leukemia ,medicine.anatomical_structure ,Nucleated cell ,Internal medicine ,Immunology ,medicine ,Immunology and Allergy ,Bone marrow ,business - Abstract
In clinical hematology, the demand for bone marrow aspiration testing is increasing. However, conventional automatic blood cell analyzers cannot completely analyze erythroblasts, and evaluation has mainly been performed by visual examination (the microscopic method). Using the CELL-DYN 4000 automatic blood cell analyzer (CD4000) (Abbott Laboratories, North Chicago, IL), specific recognition and classification of erythroblasts by DNA staining is possible. In the present study, using bone marrow blood collected from normal subjects and patients with hematological malignancy, we classified cells by the microscopic method and with the CD4000, and compared the results. Good correlations were found for total nucleated cell count (TNCC), neutrophils, lymphocytes, erythroblasts, and the myeloid series to erythroid series (M/E) ratio. It is possible to detect blasts that emerge in patients with hematological malignancy using the blast flag system installed on the CD4000. Since all of the items can be analyzed in about 80 sec with the CD4000, cells in bone marrow aspirates can be classified faster with this apparatus than by the microscopic method. Therefore, analysis of bone marrow aspirates with this apparatus appears to be very useful not only for laboratory testing but also for clinical screening.
- Published
- 2002
32. Clinical Utility of Erythrocyte Sedimentation Rate Test
- Author
-
Hiroshi Tominaga, Akira Furota, Noriyuki Tatsumi, Genzi Suganuma, Tomomi Tabuchi, and Norifumi Iwata
- Subjects
medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Erythrocyte sedimentation ,Erythrocyte sedimentation rate ,Immunology ,medicine ,Common method ,Standard methods ,Intensive care medicine ,business ,Test (assessment) - Abstract
The Erythrocyte sedimentation test is a common method used worldwide to detect acute inflammation. ICSH-WHO reference and standard methods have been routinely used for many years. However, they require much blood and long testing time over 2 hours, and several simple and rapid methods for what have recently been proposed. This paper describes advantages and disadvantages of these methods, plasma factors affecting ESR and clinical utility of which test.
- Published
- 2002
33. Virus-associated hemophagocytic syndrome due to rubella virus and varicella-zoster virus dual infection in patient with adult idiopathic thrombocytopenic purpura
- Author
-
Yasutaka Aoyama, Naoki Oiso, Nishi S, Fujino H, Yasunobu Takeoka, Masayuki Hino, Takayuki Takubo, Asao Hirose, Ki-Ryang Koh, Hirohisa Nakamae, T. Inoue, Kensuke Ohta, Noriyuki Tatsumi, and Takahisa Yamane
- Subjects
Adult ,Histiocytosis, Non-Langerhans-Cell ,Prednisolone ,viruses ,medicine.disease_cause ,Herpes Zoster ,Rubella ,Disease-Free Survival ,Herpesviridae ,Virus ,Japan ,Humans ,Medicine ,Purpura, Thrombocytopenic, Idiopathic ,medicine.diagnostic_test ,business.industry ,Varicella zoster virus ,virus diseases ,Rubella virus ,Hematology ,General Medicine ,medicine.disease ,Virology ,Thrombocytopenic purpura ,Bone marrow examination ,Immunology ,Female ,gamma-Globulins ,Hemophagocytosis ,business - Abstract
A 26-year-old woman with idiopathic thrombocytopenic purpura (ITP) was admitted to our hospital because of fever and rash. Blood tests revealed thrombocytopenia, liver dysfunction, coagulopathy, and hyperferritinemia. Bone marrow examination revealed many atypical lymphocytes and some histiocytes with hemophagocytosis. On admission she was diagnosed with rubella virus-associated hemophagocytic syndrome (VHAS), but on laboratory examination, she was seropositive for varicella-zoster virus (VZV)-IgM as well as rubella virus-IgM. She was therefore diagnosed with dual infection by rubella virus and VZV. Her simultaneous rubella virus and VZV infection may have been related to the VAHS pathogenesis. She was treated with prednisolone and gamma globulin therapy and recovered completely.
- Published
- 2001
34. RNA Extraction from Ethanol-Fixed Leukocytes Trapped on Polyethylene Terephthalate Filter for RT-PCR Analysis
- Author
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Masaharu Yokota, Noriyuki Tatsumi, Masayuki Hino, Izumi Tsuda, and Takuya Nishioka
- Subjects
Adult ,Fusion Proteins, bcr-abl ,General Biochemistry, Genetics and Molecular Biology ,Fixatives ,chemistry.chemical_compound ,Rt pcr analysis ,Leukemia, Myelogenous, Chronic, BCR-ABL Positive ,Leukocytes ,Polyethylene terephthalate ,Humans ,RNA, Messenger ,Edetic Acid ,Chromatography ,Ethanol ,Polyethylene Terephthalates ,Reverse Transcriptase Polymerase Chain Reaction ,Chemistry ,Extraction (chemistry) ,Anticoagulants ,Glyceraldehyde-3-Phosphate Dehydrogenases ,Interferon-alpha ,RNA ,RNA-Directed DNA Polymerase ,Reverse transcriptase ,Filter (aquarium) ,Biochemistry ,Spectrophotometry ,RNA extraction ,Biotechnology - Published
- 2001
35. Serum KL-6 levels in haematologic malignancies and their clinical application
- Author
-
Saori Nishiki, Noriyuki Tatsumi, Takafumi Nakao, Ki-Ryang Koh, Hirohisa Nakamae, Kensuke Ohta, Yoshimitsu Kinoshita, Takayuki Takubo, Takeo Kumura, Takahisa Yamane, Tomio Kamitani, Masayuki Hino, and Yasutaka Aoyama
- Subjects
Oncology ,medicine.medical_specialty ,business.industry ,Mucin-1 ,Mucins ,Hematology ,Antigens, Neoplasm ,Hematologic Neoplasms ,Internal medicine ,Biomarkers, Tumor ,Humans ,Medicine ,Antigens ,business ,Glycoproteins - Published
- 2001
36. Tissue Factor Pathway Inhibitor as a Universal Anticoagulant for Use in Clinical Laboratory Tests
- Author
-
Noriyuki Tatsumi, Ryuhei Tsuji, Takayuki Takubo, Masayuki Hino, and Takuya Nishioka
- Subjects
Pathology ,medicine.medical_specialty ,Erythrocytes ,Neutrophils ,Lipoproteins ,In Vitro Techniques ,Cell morphology ,General Biochemistry, Genetics and Molecular Biology ,Blood cell ,Andrology ,Tissue factor pathway inhibitor ,White blood cell ,medicine ,Humans ,Platelet ,Blood Coagulation ,Whole blood ,Prothrombin time ,Blood Cells ,medicine.diagnostic_test ,Clinical Laboratory Techniques ,Chemistry ,Anticoagulants ,General Medicine ,Blood Cell Count ,medicine.anatomical_structure ,Partial thromboplastin time - Abstract
Tissue factor pathway inhibitor (TFPI) is a protease inhibitor of extrinsic coagulation. The present study investigates the possibility of utilizing TFPI as a universal anticoagulant in clinical laboratory tests. The optimal concentration of TFPI for use in clinical laboratory tests was found to be 1 microl TFPI/ml blood (100 mmol TFPI/ml blood); the subsequent analyses were conducted at this concentration. In hematological tests, complete blood cell count and differential white blood cell count were done with an automatic blood analyzer. The results except for platelet and white blood cell counts were similar for ethylenediaminetetraacetic acid (EDTA)-treated and TFPI-treated samples. The effects of TFPI on platelet count were more pronounced when blood samples were stored at 4 degrees C than at room temperature. The effects of TFPI on cell morphology were evaluated by spreading blood samples into thin films and applying a Giemsa stain. The results showed that TFPI did not alter the morphology of blood cells. An automatic biochemical analyzer performed seventeen basic biochemical tests on serum samples and TFPI-treated plasma samples. The results of seventeen tests were comparable between TFPI-treated samples and EDTA-treated samples. The prothrombin time for TFPI-treated plasma samples was longer than that for citrated plasma samples. Nonetheless, in activated partial thromboplastin time tests, the addition of the reagent caused turbidity and partial coagulation, thus demonstrating that TFPI is not suitable for this assay. These findings suggest that although some tests cannot be performed with TFPI, this compound may be useful as a universal anticoagulant in the future.
- Published
- 2001
37. Nodal Gamma/Delta T Cell Lymphoma in Complete Remission following Allogeneic Bone Marrow Transplantation from an HLA-Matched Unrelated Donor
- Author
-
Ki-Ryang Koh, Yasutaka Aoyama, Hirohisa Nakamae, Chikahiko Sakamoto, Noriyuki Tatsumi, Takahisa Yamane, Takayuki Takubo, Ryousuke Yamamura, Kazuo Tsubaki, Taro Hasegawa, Kensuke Ota, Takeshi Inoue, and Masayuki Hino
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Adolescent ,Biopsy ,Prednisolone ,Human leukocyte antigen ,Lymphoma, T-Cell ,Bone Marrow ,immune system diseases ,hemic and lymphatic diseases ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Asparaginase ,Humans ,Transplantation, Homologous ,T-cell lymphoma ,Gamma delta T cell ,Cyclophosphamide ,Bone Marrow Transplantation ,Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor ,business.industry ,Remission Induction ,T-cell receptor ,Cytarabine ,Receptors, Antigen, T-Cell, gamma-delta ,Hematology ,General Medicine ,medicine.disease ,Combined Modality Therapy ,Peripheral T-cell lymphoma ,Lymphoma ,medicine.anatomical_structure ,Doxorubicin ,Vincristine ,Bone marrow ,Stem cell ,business - Abstract
Gamma/delta T cell lymphoma is very rare, and usually occurs as an extranodal tumor. We describe the case of a 16-year-old Japanese man with an unusual nodal gamma/delta T cell lymphoma with generalized lymphadenopathy and bone marrow involvement. No tumor involvement was observed in the liver, spleen, or nasal cavity. Examination for surface antigens on lymphoma cells revealed a unique phenotype, positive for CD3 and T cell receptor (TCR) gamma/delta, but negative for CD2. Genotypic analysis revealed the tumor to be of monoclonal origin and characterized by TCR gamma-chain gene rearrangement, but there was no rearrangement of the TCR beta-chain gene. Our patient’s tumor responded to combination chemotherapy and subsequent allogeneic bone marrow transplantation from an HLA-matched unrelated donor. He has remained well and free of disease for 35 months.
- Published
- 2001
38. [Untitled]
- Author
-
NORIYUKI TATSUMI
- Published
- 2001
39. New enzymatic method for determiningD-arabinitol in serum
- Author
-
Noriyuki Tatsumi, Takahisa Yamane, Takayuki Takubo, Kensuke Ohta, Masahiko Yabuuchi, and Masayuki Hino
- Subjects
chemistry.chemical_classification ,chemistry.chemical_compound ,Positive sample ,Enzyme ,Chromatography ,chemistry ,Reagent ,Analytical chemistry ,General Medicine ,Bacillus sp ,Formazan ,D-arabinitol ,Applied Microbiology and Biotechnology - Abstract
A new reagent has been developed to determine D-arabinitol. This utilizes D-arabinitol 2-oxidoreductase derived from Bacillus sp. with high stability, and water-soluble tetrazolium salt, that can detect NADH with high sensitivity. Since this enzyme does not react to D-mannitol, elimination of D-mannitol is unnecessary. Thus, this is a much simpler process than currently available with commercial kits use D-arabinitol 4-oxidoreductase. The within-run and between-run precisions (CV) were 2.4-6.9% and 3.1-8.7%, respectively, whilst the correlation (r) between the results obtained with our proposed method (y) and those obtained with the commercial "Arabinitec-auto" kit (x) was 0.964 (y = 1.02x + 0.933 mumol/l; n = 69). However, some samples deviated remarkably from correlation in both methods. Our analyzing accuracy is satisfactory in clinical application, as it does not miss positive sample over cut-off value. We are refining this method by investigating why some specimens are apart from correlation significantly.
- Published
- 2000
40. Ex vivoexpansion of mature human neutrophils with normal functions from purified peripheral blood CD34+haematopoietic progenitor cells
- Author
-
Seiichi Kitagawa, Ki Ryang Koh, Noriyuki Tatsumi, Fumihiko Hato, Nobuaki Sakai, Kensuke Ohta, Masayuki Hino, Hiroshi Kubota, Kenichi Suzuki, and Takahisa Yamane
- Subjects
CD34 ,Stem cell factor ,Hematology ,Granulocyte ,Biology ,Molecular biology ,Haematopoiesis ,medicine.anatomical_structure ,Immunology ,Interleukin 12 ,medicine ,Progenitor cell ,Ex vivo ,Interleukin 3 - Abstract
Purified CD34+ haematopoietic progenitor cells were cultivated with stem cell factor, interleukin 3 (IL-3), granulocyte–macrophage colony-stimulating factor (GM-CSF) and granulocyte CSF (G-CSF) for 7 d, and thereafter non-adherent cells were divided into two groups. Cells in one group (group A) were further cultivated for 7 d with four cytokines, and cells in the other group (group B) were further cultivated for 7 d with G-CSF alone. On day 14, 220-fold and 130-fold increases in the numbers of non-adherent cells were achieved for groups A and B respectively. These cell preparations contained 65% granulocytes for group A and 95% granulocytes for group B. These cells gained the ability to respond effectively with chemotaxis, phagocytosis and superoxide (O2−) release. Cells in group B were appropriately primed by G-CSF, GM-CSF, tumour necrosis factor α and IL-1β for enhanced release of . The responsiveness of these cells was identical to that of peripheral blood neutrophils, indicating that cells in group B may be in the resting state. In contrast, cells in group A were not primed by these cytokines for enhanced release of O2− and released a large amount of O2− spontaneously, indicating that cells in group A may be in the activated state. These findings indicate that mature neutrophils with normal functions were expanded ex vivo in group B and suggest that these cells could be used for possible autologous neutrophil transfusion to prevent bacterial infections during severe neutropenia after cytotoxic chemotherapy.
- Published
- 2000
41. Lymphoma in Castleman's disease, acute lymphocytic leukemia, adult T-cell leukemia and cutaneous T-cell lymphoma accompanied with high serum soluble Fas ligand levels
- Author
-
Takeo Kumura, Noriyuki Tatsumi, Masayuki Hino, Tomio Kamitani, Takayuki Takubo, Takahisa Yamane, H Ohkura, and Kensuke Ohta
- Subjects
Adult ,Male ,Anemia, Hemolytic ,Fas Ligand Protein ,T-cell leukemia ,Fas ligand ,Acute lymphocytic leukemia ,Biomarkers, Tumor ,medicine ,Humans ,Leukemia-Lymphoma, Adult T-Cell ,Multiple myeloma ,Aged ,Aged, 80 and over ,Membrane Glycoproteins ,Myeloproliferative Disorders ,biology ,business.industry ,Castleman Disease ,Lymphoma, Non-Hodgkin ,Anemia, Refractory ,Cutaneous T-cell lymphoma ,Hematology ,Middle Aged ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,medicine.disease ,BCL10 ,Lymphoma, T-Cell, Cutaneous ,Neoplasm Proteins ,Lymphoma ,Membrane glycoproteins ,Hematologic Neoplasms ,Cancer research ,biology.protein ,Female ,Multiple Myeloma ,business ,Precancerous Conditions - Published
- 2000
42. A practical quick staining method using hydrochloric acid-fast metachromatic dye for megakaryocytes
- Author
-
Noriyuki Tatsumi, Takahisa Yamane, Kishida T, K. Ota, Masayuki Hino, and T. Ota
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Indoles ,Biology ,Hematopoietic Cell Growth Factors ,Stain ,Acute megakaryoblastic leukemia ,Megakaryocyte ,Leukemia, Megakaryoblastic, Acute ,Tumor Cells, Cultured ,medicine ,Humans ,Coloring Agents ,Staining and Labeling ,Metachromasia ,Color intensity ,Bone Marrow Examination ,Cell Differentiation ,Hematology ,Hematopoietic Stem Cells ,medicine.disease ,Violet polymethine dye ,Hematologic Diseases ,Staining ,medicine.anatomical_structure ,Hematologic Neoplasms ,Female ,Hydrochloric Acid ,Bone marrow ,Megakaryocytes - Abstract
A specific stain using violet polymethine dye (VPM stain) for megakaryocytes was first developed by Kass (1995). We have modified this method for practical use in bone marrow specimens. The modified VPM stain labels megakaryocytes very well, while other marrow cells are poorly colorized. This staining procedure was more stable, and its color intensity was finer and clearer than the original. Using this stain, morphologic classification of megakaryocytes in bone marrow specimens from 11 normal and 8 myelodysplastic syndrome (MDS) patients was performed. Many megakaryocytes observed in MDS patients were juvenile compared with normal subjects according to their morphology. Blasts from acute megakaryoblastic leukemia (M7) and from a megakaryoblastic cell line (Mo7e) were also clearly stained with our method. This staining method is practical and very useful for rapid identification of megakaryocyte distribution and morphology.
- Published
- 2000
43. Synthetic polymer sulphonated polyisoprene as a universal anticoagulant for laboratory testing
- Author
-
Takahisa Yamane, Noriyuki Tatsumi, Takayuki Takubo, Kensuke Ohta, Yoshimitsu Kinoshita, Masayuki Hino, and Tomohiro Samori
- Subjects
Adult ,Male ,Microbiology (medical) ,Platelet Aggregation ,Platelet aggregation ,Polymers ,medicine.drug_class ,Clinical Biochemistry ,Electrolyte ,Laboratory testing ,Electrolytes ,Leukocyte Count ,Hemiterpenes ,Kanamycin ,Pentanes ,Butadienes ,medicine ,Humans ,Immunology and Allergy ,Organic chemistry ,Platelet ,Edetic Acid ,Aged ,Chelating Agents ,Aspirin ,Chromatography ,Platelet Count ,Chemistry ,Biochemistry (medical) ,Anticoagulant ,Public Health, Environmental and Occupational Health ,Anticoagulants ,Original Articles ,Hematology ,Middle Aged ,Synthetic polymer ,Anti-Bacterial Agents ,Blood Cell Count ,Medical Laboratory Technology ,Chemistry, Clinical ,Female ,medicine.drug - Abstract
To evaluate the feasibility of a synthetic polymer, sulphonated polyisoprene (SPIP), to be used as a new laboratory anticoagulant, hematological items were compared between blood samples anticoagulated with EDTA and SPIP, as were biochemical and electrolyte items between serum samples and SPIP‐anticoagulated blood. Among hematological items, the average platelet count of the SPIP group was significantly lower than that of the EDTA group, due to platelet aggregation in the SPIP group. Addition of kanamycin to SPIP, but not of aspirin or prostaglandin E1, restored platelet count to about 80% of that in the EDTA group, and increased coefficients of correlation with the EDTA group for most hematological items examined. For biochemical and electrolyte items, high (> 0.95) correlation coefficients were obtained for 15 of 18 items between the serum and SPIP‐anticoagulated samples. However, for some of these items, the addition of kanamycin decreased coefficients of correlation. Thus, SPIPs are excellent candidates for new anticoagulants that can be used for evaluation of hematological, biochemical, and electrolyte items with a single test tube in routine laboratory work. However, some improvements are required for use of kanamycin as an additive to inhibit platelet aggregation induced by SPIP, and in maintaining reliability of biochemical and electrolyte measurements. J. Clin. Lab. Anal. 14:180–187, 2000. © 2000 Wiley‐Liss, Inc.
- Published
- 2000
44. MATURITY OF RETICULOCYTES IN VARIOUS HEMATOLOGICAL DISORDERS
- Author
-
Noriyuki Tatsumi and Izumi Tsuda
- Subjects
Hematological disorders ,Pediatrics ,medicine.medical_specialty ,Reticulocytes ,business.industry ,Erythrocyte Count ,Humans ,Medicine ,Hematology ,General Medicine ,business ,Hematologic Diseases ,Maturity (finance) - Published
- 2009
45. Relative distribution of myosin, actin and α-actinin in surface-activated, spreading platelets
- Author
-
Masayuki Hino, Kenichi Suzuki, Noriyuki Tatsumi, and Takayuki Takubo
- Subjects
Blood Platelets ,Chemistry ,Relative distribution ,Hematology ,General Medicine ,Actinin ,Myosins ,Platelet Activation ,Actins ,Actinin, alpha 1 ,Microscopy, Fluorescence ,Myosin ,Biophysics ,Humans ,Platelet ,Platelet activation ,Actin - Published
- 2009
46. A new direction in automated laboratory testing in Japan: five years of experience with total laboratory automation system management
- Author
-
Noriyuki Tatsumi, Kiyoshi Okuda, and Izumi Tsuda
- Subjects
medicine.medical_specialty ,Inservice Training ,Point-of-Care Systems ,Clinical Biochemistry ,Context (language use) ,computer.software_genre ,Biochemistry ,Disasters ,Hospitals, University ,Automation ,Japan ,Systems management ,Health care ,Medicine ,Operations management ,Clinical Laboratory Techniques ,business.industry ,Public health ,Politics ,Biochemistry (medical) ,General Medicine ,Systems Integration ,Laboratory automation ,Managed care ,System integration ,Health Expenditures ,Laboratories ,business ,computer - Abstract
The introduction of integrated laboratory systems has proceeded rapidly in Japan in these 15 years, but they require large initial investment for installation and do not always succeed in reducing laboratory cost. We also experienced three major events that taught us that total laboratory systems are not always effective: these were an earthquake, a nerve gas attack, and an outbreak of food poisoning. Political changes in the national health care system in Japan have forced the cutting of expenses for laboratory testing. In this context, cost-effective laboratory testing has been considered, and many hospitals have replaced total laboratory systems with small laboratory systems. Our University Hospital introduced a mini-lab system consisting of compact instruments to increase laboratory efficiency, and we have begun point-of-care testing education for medical students. This combination enables rapid and convenient testing, and is responsive to the political changes in the Japanese health care system.
- Published
- 1999
47. HLA class II polymorphism in Thai patients with non-Hodgkin’s lymphoma
- Author
-
Suwanasophon C, O. Nathalang, Noriyuki Tatsumi, Wichai Prayoonwiwat, M. Hino, T. Sriphaisal, and Takahisa Yamane
- Subjects
musculoskeletal diseases ,Incidence (epidemiology) ,Immunology ,Disease ,Biology ,medicine.disease ,Non-Hodgkin's lymphoma ,Lymphoma ,law.invention ,immune system diseases ,law ,Polymorphism (computer science) ,hemic and lymphatic diseases ,Genetics ,medicine ,Primer (molecular biology) ,Allele ,skin and connective tissue diseases ,Polymerase chain reaction - Abstract
The distribution of HLA-DRB1 alleles and DQB1 alleles in 100 Thai patients with non-Hodgkin's lymphoma (NHL) was analysed using the polymerase chain reaction with sequence-specific primer (PCR-SSP) method, and the association between the disease and the presence of certain HLA class II alleles was investigated. The frequencies of HLA-DRB1*1502 and DRB1*09012 were increased while those of DRB1*0404, DRB1*0803 and DRB1*1106 were decreased. On the other hand, the incidence of HLA-DQB1 alleles was similar to that in the normal population. Interestingly, only HLA-DRB1*1502 showed a significant positive association with NHL, especially in patients < or / = 45 years and in male patients. It is concluded that the DRB1*1502 allele may contribute to NHL susceptibility in the Thai population. However, further studies on the functional roles of the HLA class II alleles are necessary to elucidate NHL susceptibility.
- Published
- 1999
48. Limited polymorphism in the HLA-DOA gene
- Author
-
Taeko Naruse, Hidetoshi Inoko, Tatsuya Anzai, M. Kagiya, N. Takashige, Gen Isshiki, N. Nabeya, H. Kawata, Noriyuki Tatsumi, and Yoshisuke Nose
- Subjects
chemistry.chemical_classification ,Genetics ,Immunology ,General Medicine ,Biology ,Biochemistry ,DNA sequencing ,Amino acid ,Class II gene ,chemistry ,HLA-DOA ,Immunology and Allergy ,Nucleotide ,Typing ,Allele ,Gene - Abstract
The HLA-DO molecule, a heterodimer consisting of two novel members of the class II gene family, DOA and DOB, has recently been suggested to function as an important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule. In this study, we have analyzed genetic polymorphism and allelic variation of the HLA-DOA gene in 37 HLA class II homozygous typing cells using the direct DNA sequencing technique. As a result, we recognized at least eight allelic variations, DOA*01011, *0101201, *0101202, *0101203, *01013, *0101401, *0101402 and *01015. None of them, however, result in amino acid substitution. The HLA-DOA gene has been identified in other mammals as well, and the nucleotide sequences were well conserved among these species. These results suggest that the DOA molecule has undergone strong selective pressure to preserve functional structure and conformation required for interaction with the DM molecule, preventing non-synonymous amino acid substituiton Note.
- Published
- 1999
49. A comparative study of administration methods of granisetron injection used to treat nausea/vomiting induced by cancer chemotherapy without cisplatin in tumors of hematopoietic organs
- Author
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Noriyuki Tatsumi and Tohru Masaoka
- Subjects
Pharmacology ,Cisplatin ,Cancer Research ,Chemotherapy ,Cyclophosphamide ,business.industry ,Nausea ,medicine.drug_class ,medicine.medical_treatment ,Toxicology ,Granisetron ,Nitrogen mustard ,chemistry.chemical_compound ,Oncology ,chemistry ,Anesthesia ,Vomiting ,Medicine ,Antiemetic ,Pharmacology (medical) ,medicine.symptom ,business ,medicine.drug - Abstract
Purpose: The antiemetic effect of granisetron injection at a dose of 40 μg/kg used in the treatment of nausea/vomiting induced by multidrug combined cancer chemotherapy excluding cisplatin in patients with tumors of hematopoietic organs was evaluated by comparing a 30-min infusion and a slow intravenous injection given over 30 s. Methods: A two-group random-allocation comparative study was performed with the cooperation of multiple institutions using a central registration system. Results: In the treatment of acute clinical symptoms, appetite was described as “similar to that during good health” by 61.1% of patients (55/93) in the instillation group and by 47.3% (44/93) in the slow injection group, a significant advantage in the infusion group. However, no significant differences in the number of episodes of vomiting, the severity of nausea or clinical efficacy were found. In the final clinical evaluation and assessment of usefulness based on the subjective judgement of physicians throughout the entire therapeutic period, no differences were discernible. No side effects were reported for either method and there was no indication of a sex difference concerning efficacy. However, the efficacy in patients with an anemic tendency was slightly inferior. Conclusions: The maintenance of appetite during the administration of anticancer drugs is very important to maintain patients' daily activities and quality of life. The present results support the usefulness of infusion of granisetron as an administration method during chemotherapy for malignant hemopathy.
- Published
- 1999
50. Cytokine-Specific Activation of Distinct Mitogen-Activated Protein Kinase Subtype Cascades in Human Neutrophils Stimulated by Granulocyte Colony-Stimulating Factor, Granulocyte-Macrophage Colony-Stimulating Factor, and Tumor Necrosis Factor-
- Author
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Seiichi Kitagawa, Fumihiko Hato, Masayuki Hino, Noriyuki Tatsumi, and Kenichi Suzuki
- Subjects
MAPK/ERK pathway ,medicine.medical_specialty ,Kinase ,MEK inhibitor ,medicine.medical_treatment ,Immunology ,MAP Kinase Kinase 3 ,Cell Biology ,Hematology ,Mitogen-activated protein kinase kinase ,Biology ,Biochemistry ,Molecular biology ,Endocrinology ,Cytokine ,Internal medicine ,Mitogen-activated protein kinase ,medicine ,biology.protein ,Protein kinase A - Abstract
To clarify the differences of the signaling pathways used by granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), and tumor necrosis factor- (TNF), we investigated activation of mitogen-activated protein kinase (MAPK) subtype cascades in human neutrophils stimulated by these cytokines. G-CSF exclusively tyrosine-phosphorylated extracellular signal-regulated kinase (ERK). GM-CSF tyrosine-phosphorylated ERK strongly and p38 MAPK weakly, whereas TNF tyrosine-phosphorylated p38 MAPK strongly and ERK weakly. Consistent with these findings, MEK, an upstream kinase of ERK, was phosphorylated by G-CSF, GM-CSF, and TNF, whereas MKK3/MKK6, an upstream kinase of p38 MAPK, was phosphorylated by GM-CSF and TNF, but not by G-CSF. The potency of these cytokines to phosphorylate ERK and MEK was GM-CSF > G-CSF > TNF, whereas that to phosphorylate p38 MAPK and MKK3/MKK6 was TNF > GM-CSF. C-Jun amino-terminal kinase (JNK) was not tyrosine-phosphorylated by any cytokine despite the existence of JNK proteins in human neutrophils, whereas it was tyrosine-phosphorylated by TNF in undifferentiated and all-trans retinoic acid-differentiated HL-60 cells. Increased phosphorylation of ERK or p38 MAPK was detected within 1 to 5 minutes after stimulation with each cytokine and was dependent on the concentrations of cytokines used. MEK inhibitor (PD98059) reduced tyrosine phosphorylation of ERK, but not p38 MAPK, induced by G-CSF, GM-CSF, or TNF. GM-CSF– or TNF-induced superoxide (O2−) release was inhibited by p38 MAPK inhibitor (SB203580) in a dose-dependent manner, suggesting the possible involvement of p38 MAPK in GM-CSF– or TNF-induced O2− release. The results indicate that G-CSF, GM-CSF, and TNF activate the overlapping but distinct MAPK subtype cascades in human neutrophils and suggest that the differential activation of ERK and p38 MAPK cascades may explain the differences of the effects of these cytokines on human neutrophil functions.
- Published
- 1999
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