Search

Your search keyword '"Nordling, Åsa"' showing total 21 results
Start Over Author "Nordling, Åsa"
21 results on '"Nordling, Åsa"'

2. A 12-gene pharmacogenetic panel to prevent adverse drug reactions: an open-label, multicentre, controlled, cluster-randomised crossover implementation study

Author

Buunk, Annemarie, Goossens, Hanneke, Baas, Gert, Algera, Maartje, Schuil-Vlassak, Evelyn, Ambagts, Thijs, De Hoog-Schouten, Leonie, Musaafir, Sara, Bosch, Roelof, Tjong, Carol, Steeman, Sanne, Van der Plas, Martine, Baldew, Glenn, Den Hollander, Iris, De Waal, Zacharias, Heijn, Aurele, Nelemans, Leen, Kouwen-Lubbers, Kirsten, Van Leeuwen, Maartje, Hoogenboom, Sacha, Van Doremalen, Jacobine, Ton, Célin, Beetstra, Bastien, Meijs, Veronique, Dikken, Jan, Dubero, Dasha, Slager, Mark, Houben, Tom, Kanis, Thomas, Overmars, Wietske, Nijenhuis, Marga, Steffens, Michael, Bergs, Ingmar, Karamperis, Kariofyllis, Siamoglou, Stavroula, Ivantsik, Ouliana, Samiou, Georgia-Chryssa, Kordou, Zoe, Tsermpini, Evira, Ferentinos, Panagiotis, Karaivazoglou, Aikaterini, Rigas, George, Gerasimou, Harilaos, Voukelatou, Georgia, Georgila, Eleni, Tsermpini, Evangelia Eirini, Mendrinou, Efrossyni, Chalikiopoulou, Konstantina, Kolliopoulou, Alexandra, Mitropoulos, Konstantinos, Stratopoulos, Apostolos, Liopetas, Ioannis, Tsikrika, Athina, Barba, Evangelia, Emmanouil, Georgia, Stamopoulou, Theano, Stathoulias, Andreas, Giannopoulos, Panagiotis, Kanellakis, Filippos, Bartsakoulia, Marina, Katsila, Theodora, Douzenis, Athanassios, Gourzis, Filippos, Assimakopoulos, Konstantinos, Bignucolo, Alessia, Dal Cin, Lisa, Comello, Francesco, Mezzalira, Silvia, Puglisi, Fabio, Spina, Michele, Foltran, Luisa, Guardascione, Michela, Buonadonna, Angela, Bartoletti, Michele, Corsetti, Serena, Ongaro, Elena, Da Ros, Lucia, Bolzonello, Silvia, Spazzapan, Simon, Freschi, Andrea, Di Nardo, Paola, Palazzari, Elisa, Navarria, Federico, Innocente, Roberto, Berretta, Massimiliano, D'Andrea, Mario, Angelini, Francesco, Diraimo, Tania, Favaretto, Adolfo, Dávila-Fajardo, Cristina Lucía, Díaz-Villamarín, Xando, Martínez-González, Luis Javier, Antúnez-Rodríguez, Alba, Moreno-Escobar, Eduardo, Fernández-Gómez, Ana Estefanía, García-Navas, Paloma, Bautista-Pavés, Alicia Bautista Pavés, Burillo-Gómez, Francisco, Villegas-Rodríguez, Inmaculada, Sánchez-Ramos, Jesús Gabriel, Antolinos-Pérez, Mª José, Rivera, Ricardo, Martínez-Huertas, Susana, Thomas-Carazo, Jesús, Yañez-Sanchez, Jose Julio, Blancas-López-Barajas, Mª Isabel, García-Orta, Rocío, González-Astorga, Beatriz, Rodríguez-González, Carlos José, Ruiz-Carazo, Francisco Javier, Pérez-Campos, Manuel, Cano-Herrera, Irene, Herrera, Rosa, Gil-Jiménez, Teresa, Delgado-Ureña, Mª Teresa, Triviño-Juarez, Jose Matías, Campos-Velázquez, Salustiano, Alcántara- Espadafor, Silvia, Moreno Aguilar, Maria Rosario, Ontiveros- Ortega, Maria Carmen, Carnerero-Córdoba, Lidia, Guerrero-Jiménez, Margarita, Legeren- Álvarez, Marta, Yélamos-Vargas, Marisol, Castillo-Pérez, Isabel, Aomar-Millán, Ismael, Anguita-Romero, Manuel, Sánchez-García, María José, Sequero-Lopez, Silvia, Faro-Miguez, Naya, López-Fernández, Silvia, Leyva-Ferrer, Rosario Nieves, Herrera-Gómez, Norberto, Pertejo-Manzano, Laura, Pérez-Gutierrez, Eva Mª, Martín-de la Higuera, Antonio J., Plaza-Carrera, Jose, Baena-Garzón, Flor, Toledo-Frías, Pablo, Cruz-Valero, Inés, Chacón-McWeeny, Verónica, Gallardo- Sánchez, Isabel, Arrebola, Antonio, Guillén-Zafra, Lucía, Ceballos-Torres, Ángel, Guardia-Mancilla, Plácido, Guirao-Arrabal, Emilio, Canterero-Hinojosa, Jesús, Velasco-Fuentes, Sara, Sánchez- Cano, Daniel, Aguilar-Jaldo, Mª del Pilar, Caballero-Borrego, Juan, Praznik, Monika, Slapšak, Urška, Voncina, Blaz, Rajter, Branka, Škrinjar, Andrej, Marjetic Ulcakar, Angelika, Zidanšek, Anja, Stegne Ignjatvic, Tea, Mazej Poredoš, Barbara, Vivod Pecnik, Živka, Poplas Susic, Tonka, Juteršek, Milojka, Klen, Jasna, Skoporc, Janja, Kotar, Tjaša, Petek Šter, Marija, Zvezdana Dernovšk, Mojca, Mlinšek, Gregor, Miklavcic, Petra, Plemenitaš Ilješ, Anja, Grašic Kuhar, Cvetka, Oblak, Irena, Stražišar, Branka, Štrbac, Danijela, Matos, Erika, Mencinger, Marina, Vrbnjak, Marko, Saje, Marko, Radovanovic, Mirjana, Jeras, Katja, Bukovec, Lucija, Terzic, Tea, Minichmayr, Iris, Nanah, Abdulaziz, Nielsen, Elisabet, Zou, Yuanxi, Lauschke, Volker, Johansson, Inger, Zhou, Yitian, Nordling, Åsa, Aigner, Christof, Dames-Ludwig, Marlies, Monteforte, Rossella, Sunder-Plassmann, Raute, Steinhauser, Corinna, Sengoelge, Guerkan, Winnicki, Wolfgang, Schmidt, Alice, Vasileios, Fragoulakis, Fontana, Vanessa, Hanson, Anita, Little, Margaret, Hornby, Rachael, Dello Russo, Cinzia, French, Stephanie, Hampson, Jamie, Gumustekin, Mukaddes, Anyfantis, George, Hampson, Lucy, Lewis, David, Westhead, Ruth, Prince, Clare, Rajasingam, Arjunan, Swen, Jesse J, van der Wouden, Cathelijne H, Manson, Lisanne EN, Abdullah-Koolmees, Heshu, Blagec, Kathrin, Blagus, Tanja, Böhringer, Stefan, Cambon-Thomsen, Anne, Cecchin, Erika, Cheung, Ka-Chun, Deneer, Vera HM, Dupui, Mathilde, Ingelman-Sundberg, Magnus, Jonsson, Siv, Joefield-Roka, Candace, Just, Katja S, Karlsson, Mats O, Konta, Lidija, Koopmann, Rudolf, Kriek, Marjolein, Lehr, Thorsten, Mitropoulou, Christina, Rial-Sebbag, Emmanuelle, Rollinson, Victoria, Roncato, Rossana, Samwald, Matthias, Schaeffeler, Elke, Skokou, Maria, Schwab, Matthias, Steinberger, Daniela, Stingl, Julia C, Tremmel, Roman, Turner, Richard M, van Rhenen, Mandy H, Dávila Fajardo, Cristina L, Dolžan, Vita, Patrinos, George P, Pirmohamed, Munir, Sunder-Plassmann, Gere, Toffoli, Giuseppe, and Guchelaar, Henk-Jan

Published
2023
Full Text
View/download PDF

5. Mechanistic, Functional, and Clinical Aspects of Pro‐inflammatory Cytokine Mediated Regulation of ADME Gene Expression in 3D Human Liver Spheroids.

Author

Klöditz, Katharina, Tewolde, Eida, Nordling, Åsa, and Ingelman‐Sundberg, Magnus

Subjects
GENETIC regulation, NUCLEAR proteins, GENE expression, DRUG metabolism, LIVER, GENE expression profiling
Abstract

During systemic inflammation, pro‐inflammatory cytokines alter metabolism and transport of drugs affecting the clinical outcome. We used an in vivo like human 3D liver spheroid model to study the effects and mechanisms of pro‐inflammatory cytokines on the expression of 9 different genes encoding enzymes responsible for the metabolism of > 90% of clinically used drugs. Treatment of spheroids with pathophysiologically relevant concentrations of IL‐1β, IL‐6, or TNFα resulted in a pronounced decrease in mRNA expression of CYP3A4 and UGT2B10 within 5 hours. The reduction of CYP1A2, CYP2C9, CYP2C19, and CYP2D6 mRNA expression was less pronounced, whereas the pro‐inflammatory cytokines caused increased CYP2E1, and UGT1A3 mRNA expression. The cytokines did not influence expression of key nuclear proteins, nor the activities of specific kinases involved in the regulation of genes encoding drug metabolizing enzymes. However, ruxolitinib, a JAK1/2 inhibitor, inhibited the IL‐6 dependent increase in CYP2E1 and the decrease in CYP3A4 and UGT2B10 mRNA expression. We evaluated the effect of TNFα in hepatocytes in 2D plates and found a rapid decrease in drug‐metabolizing enzyme mRNA both in the absence or presence of the cytokines. Taken together, these data suggest that pro‐inflammatory cytokines regulate multiple gene‐ and cytokine‐specific events seen in in vivo and in 3D but not in 2D liver models. We propose that the 3D spheroid system is suitable for the prediction of drug metabolism under conditions of inflammation and constitutes a versatile system for short‐ and long‐term preclinical and mechanistic studies of cytokine‐induced changes in drug metabolism. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

8. A 12-gene pharmacogenetic panel to prevent adverse drug reactions: an open-label, multicentre, controlled, cluster-randomised crossover implementation study

Author

Swen, Jesse J, van der Wouden, Cathelijne H, Manson, Lisanne EN, Abdullah-Koolmees, Heshu, Blagec, Kathrin, Blagus, Tanja, Böhringer, Stefan, Cambon-Thomsen, Anne, Cecchin, Erika, Cheung, Ka-Chun, Deneer, Vera HM, Dupui, Mathilde, Ingelman-Sundberg, Magnus, Jonsson, Siv, Joefield-Roka, Candace, Just, Katja S, Karlsson, Mats O, Konta, Lidija, Koopmann, Rudolf, Kriek, Marjolein, Lehr, Thorsten, Mitropoulou, Christina, Rial-Sebbag, Emmanuelle, Rollinson, Victoria, Roncato, Rossana, Samwald, Matthias, Schaeffeler, Elke, Skokou, Maria, Schwab, Matthias, Steinberger, Daniela, Stingl, Julia C, Tremmel, Roman, Turner, Richard M, van Rhenen, Mandy H, Dávila Fajardo, Cristina L, Dolžan, Vita, Patrinos, George P, Pirmohamed, Munir, Sunder-Plassmann, Gere, Toffoli, Giuseppe, Guchelaar, Henk-Jan, Buunk, Annemarie, Goossens, Hanneke, Baas, Gert, Algera, Maartje, Schuil-Vlassak, Evelyn, Ambagts, Thijs, De Hoog-Schouten, Leonie, Musaafir, Sara, Bosch, Roelof, Tjong, Carol, Steeman, Sanne, Van der Plas, Martine, Baldew, Glenn, Den Hollander, Iris, De Waal, Zacharias, Heijn, Aurele, Nelemans, Leen, Kouwen-Lubbers, Kirsten, Van Leeuwen, Maartje, Hoogenboom, Sacha, Van Doremalen, Jacobine, Ton, Célin, Beetstra, Bastien, Meijs, Veronique, Dikken, Jan, Dubero, Dasha, Slager, Mark, Houben, Tom, Kanis, Thomas, Overmars, Wietske, Nijenhuis, Marga, Steffens, Michael, Bergs, Ingmar, Karamperis, Kariofyllis, Siamoglou, Stavroula, Ivantsik, Ouliana, Samiou, Georgia-Chryssa, Kordou, Zoe, Tsermpini, Evira, Ferentinos, Panagiotis, Karaivazoglou, Aikaterini, Rigas, George, Gerasimou, Harilaos, Voukelatou, Georgia, Georgila, Eleni, Tsermpini, Evangelia Eirini, Mendrinou, Efrossyni, Chalikiopoulou, Konstantina, Kolliopoulou, Alexandra, Mitropoulos, Konstantinos, Stratopoulos, Apostolos, Liopetas, Ioannis, Tsikrika, Athina, Barba, Evangelia, Emmanouil, Georgia, Stamopoulou, Theano, Stathoulias, Andreas, Giannopoulos, Panagiotis, Kanellakis, Filippos, Bartsakoulia, Marina, Katsila, Theodora, Douzenis, Athanassios, Gourzis, Filippos, Assimakopoulos, Konstantinos, Bignucolo, Alessia, Dal Cin, Lisa, Comello, Francesco, Mezzalira, Silvia, Puglisi, Fabio, Spina, Michele, Foltran, Luisa, Guardascione, Michela, Buonadonna, Angela, Bartoletti, Michele, Corsetti, Serena, Ongaro, Elena, Da Ros, Lucia, Bolzonello, Silvia, Spazzapan, Simon, Freschi, Andrea, Di Nardo, Paola, Palazzari, Elisa, Navarria, Federico, Innocente, Roberto, Berretta, Massimiliano, D'Andrea, Mario, Angelini, Francesco, Diraimo, Tania, Favaretto, Adolfo, Dávila-Fajardo, Cristina Lucía, Díaz-Villamarín, Xando, Martínez-González, Luis Javier, Antúnez-Rodríguez, Alba, Moreno-Escobar, Eduardo, Fernández-Gonzalez, Ana Estefanía, García-Navas, Paloma, Bautista-Pavés, Alicia Bautista Pavés, Burillo-Gómez, Francisco, Villegas-Rodríguez, Inmaculada, Sánchez-Ramos, Jesús Gabriel, Antolinos-Pérez, Mª José, Rivera, Ricardo, Martínez-Huertas, Susana, Thomas-, Jesús, Carazo, Jose Julio, Yañez-Sanchez, Mª Isabel, Blancas-López-Navajas, Rocío, García-Orta, Beatriz, González-Astorga, Carlos José, Rodríguez-González, Francisco Javier, Ruiz-Carazo, Manuel, López-Pérez, Irene, Cano-Herrera, Rosa, Herrera, Teresa, Gil-Jiménez, Delgado-Ureña, Mª Teresa, Triviño-Juarez, Jose Matías, Campos-Velázquez, Salustiano, Alcántara-Espadafor, Silvia, Moreno Aguilar, Maria Rosario, Ontiveros-Ortega, Maria Carmen, Carnerero-Córdoba, Lidia, Guerrero-Jiménez, Margarita, Legeren-Álvarez, Marta, Yélamos-Vargas, Marisol, Castillo-Pérez, Isabel, Aomar-Millán, Ismael, Anguita-Romero, Manuel, Sánchez-García, María José, Sequero-Lopez, Silvia, Faro-Miguez, Naya, López-Fernández, Silvia, Leyva-Ferrer, Rosario Nieves, Herrera-Gómez, Norberto, Pertejo-Manzano, Laura, Pérez-Gutierrez, Eva Mª, Martín-de la Higuera, Antonio J., Plaza-Carrera, Jose, Baena-Garzón, Flor, Toledo-Frías, Pablo, Cruz-Valero, Inés, Chacón-McWeeny, Verónica, Gallardo-Sánchez, Isabel, Arrebola, Antonio, Guillén-Zafra, Lucía, Ceballos-Torres, Ángel, Guardia-Mancilla, Plácido, Guirao-Arrabal, Emilio, Canterero-Hinojosa, Jesús, Velasco-Fuentes, Sara, Sánchez-Cano, Daniel, Aguilar-Jaldo, Mª del Pilar, Caballero-Borrego, Juan, Praznik, Monika, Slapšak, Urška, Vončina, Blaz, Rajter, Branka, Škrinjar, Andrej, Marjetič Ulčakar, Angelika, Zidanšek, Anja, Stegne Ignjatvič, Tea, Mazej Poredoš, Barbara, Vivod Pečnik, Živka, Poplas Susič, Tonka, Juteršek, Milojka, Klen, Jasna, Skoporc, Janja, Kotar, Tjaša, Petek Šter, Marija, Zvezdana Dernovšk, Mojca, Klen, Jasna, Mlinšek, Gregor, Miklavčič, Petra, Plemenitaš Ilješ, Anja, Grašič Kuhar, Cvetka, Oblak, Irena, Stražišar, Branka, Štrbac, Danijela, Matos, Erika, Mencinger, Marina, Vrbnjak, Marko, Saje, Marko, Radovanovič, Mirjana, Jeras, Katja, Bukovec, Lucija, Terzič, Tea, Minichmayr, Iris, Nanah, Abdulaziz, Nielsen, Elisabet, Zou, Yuanxi, Lauschke, Volker, Johansson, Inger, Zhou, Yitian, Nordling, Åsa, Aigner, Christof, Dames-Ludwig, Marlies, Monteforte, Rossella, Sunder-Plassmann, Raute, Steinhauser, Corinna, Sengoelge, Guerkan, Winnicki, Wolfgang, Schmidt, Alice, Vasileios, Fragoulakis, Fontana, Vanessa, Hanson, Anita, Little, Margaret, Hornby, Rachael, Dello Russo, Cinzia, French, Stephanie, Hampson, Jamie, Gumustekin, Mukaddes, Anyfantis, George, Hampson, Lucy, Lewis, David, Westhead, Ruth, Prince, Clare, and Rajasingam, Arjunan

Abstract

The benefit of pharmacogenetic testing before starting drug therapy has been well documented for several single gene–drug combinations. However, the clinical utility of a pre-emptive genotyping strategy using a pharmacogenetic panel has not been rigorously assessed.

Published
2023
Full Text
View/download PDF

10. Clinically Relevant Cytochrome P450 3A4 Induction Mechanisms and Drug Screening in Three‐Dimensional Spheroid Cultures of Primary Human Hepatocytes

Author

Hendriks, Delilah F.G., primary, Vorrink, Sabine U., additional, Smutny, Tomas, additional, Sim, Sarah C., additional, Nordling, Åsa, additional, Ullah, Shahid, additional, Kumondai, Masaki, additional, Jones, Barry C., additional, Johansson, Inger, additional, Andersson, Tommy B., additional, Lauschke, Volker M., additional, and Ingelman‐Sundberg, Magnus, additional

Published
2020
Full Text
View/download PDF

12. Perioperativa åtgärder för att lindra och förebygga postoperativ halsont och heshet hos patienter som genomgått generell anestesi

Author

Ekdahl, Manne and Nordling, Åsa

Subjects
Postoperative complications, Sore throat, Hoarseness, Heshet, Omvårdnad, General anesthesia, Generell anestesi, Nursing, Intubation, Halsont, Postoperativa komplikationer
Abstract

Bakgrund: Halsont och heshet är några av de vanligaste postoperativa komplikationerna hos patienter som erhållit endotrakealtub eller larynxmask under generell anestesi. Syfte: Att kartlägga anestesisjuksköterskans perioperativa åtgärder för att förebygga och lindra patientens postoperativa halsont och heshet. Metod: En litteraturöversikt med deskriptiv design. Resultat sammanställdes utifrån 18 granskade artiklar av kvantitativ ansats, eftersökta i databaserna PudMed och Cinahl. Resultat: Litteraturöversikten visade flertalet åtgärder som kunde minska både incidens och svårighetsgrad av postoperativ halsont och heshet. En mindre endotrakealtub, användning av kontinuerlig tryckregulator, administrering av glukokortikoider och användning av larynxmask gav minskad incidens av halsont och heshet. Även videolaryngoskop, minskad kraft vid extraktion av ledare och vissa naturläkemedel visade sig minska incidensen. Åtgärderna visade på goda effekter i nära anslutning till operationen och inom första postoperativa dygnet, emellertid gav få artiklar resultat med bibehållen effekt över tid. Slutsats: Studien gav goda indikationer för åtgärder som kunde minska patienters förekomst av halsont och heshet och därigenom deras lidande. Resultatet visade på stor spridning vilket gjorde det svårt bedöma vilka åtgärder som var lämpliga att applicera i kliniken. Att minska storleken på endotrakealtuben och använda larynxmask när operationen tillåter var åtgärder som ansågs användbara. Även användning av glukokortikoider/antiinflammatoriska läkemedel samt att i större utsträckning utnyttja videolaryngoskopstekniken ansågs som adekvata åtgärder för att minska patientens lidande orsakat av postoperativ halsont och heshet. Background: Sore throat and hoarseness are some of the most common postoperative complications in patients receiving endotracheal tube or laryngeal mask during general anesthesia. Aim: To investigate the anesthetic nurse's perioperative measures to prevent and alleviate the patient's suffering from postoperative sore throat and hoarseness. Method: A literature review with descriptive design. Results were compiled based on 18 articles of quantitative approaches, requested in the databases PudMed and Cinahl. Result: The literature review showed several measures that could reduce both incidence and severity of postoperative sore throat and hoarseness. A minor endotracheal tube, the use of continuous cuff pressure regulator, administration of glucocorticoids and use of laryngeal mask reduced the incidence of sore throat and hoarseness. Also, video laryngoscopes, reduced force when extracting stylet removal and some herbal medications seemed to give good results in reducing the incidence. The measures showed good effects close to surgery and within the first postoperative day, however, few studies gave results with retained effect over time. Conclusions: The study provided good indications for measures that could reduce patients' incidence of sore throat and hoarseness and thereby their suffering. The results showed a large spread, which made it difficult to assess which measures were suitable for application in the clinic. Reducing the size of the endotracheal tube and using the laryngeal mask when the operation allows was implications that considered useful. Also, the use of glucocorticoids / anti-inflammatory drugs and to a greater extent utilizing the video laryngoscope technique was considered adequate measures to reduce the suffering of patients caused by postoperative sore throat and hoarseness.

Published
2019

14. Livskvalitet hos personer som lever med en implanterbar defibrillator (ICD), ur ett köns- och åldersperspektiv : En beskrivande litteraturstudie

Author

Bjerke, Sofia and Nordling, Åsa

Subjects
sex differences, age factors, Medicin och hälsovetenskap, Implanterbar defibrillator, quality of life, livskvalitet, åldersskillnader, könsskillnader, Defibrillators implantable, Medical and Health Sciences
Abstract

Background: The ICD controls the heart rhythm and reacts to serious heart rhythm abnormalities and trigger, if necessary, a defibrillation to restore the heart to normal rhythm. Living with an ICD can provide major changes in everyday life. Nurse´s important role regards to provide relevant patient education to include promoting the health and quality of life (QOL) for these individuals. Purpose: To describe QOL for people living with an ICD, from a gender and age perspective, and to examine the included articles selection strategy and research group. Method: The result of this descriptive literature review compiled from eleven quantitative scientific articles, sought in PubMed and Scopus. Main Results: Women reported lower QOL then men linked to impaired mental, physical and social function. Also a greater concern and anxiety were found in women. Mental illness affected mainly young people, who also experienced a lower acceptance to the device than older ICD- receivers. Older people reported less concern that the ICD would deliver a shock. However, a reduced QOL related to higher incidence of physical impairment, were noticed in the elderly ICD- receivers compared to the younger. Four articles specified their selection strategy. All reported the number of participants and how many were men and women. The age range of participants was specified in five articles. Conclusion: Impacts on QOL could be discerned to different gender and ages. These findings confirmed the need for health professionals to access more holistic patient- education programs that focus not only on the technical aspects of living with an ICD but more on the individual impact. Bakgrund: En ICD kontrollerar hjärtrytmen, reagerar på allvarliga hjärtrytmrubbningar och utlöser vid behov en defibrillering för att återställa normal hjärtrytm. Att leva med en ICD kan innebära stora omställningar i det vardagliga livet. Sjuksköterskan har en betydande roll i omvårdnaden kring dessa personer där bland annat relevant patientutbildning är viktig för att främja personers hälsa och livskvalitet. Syfte: Syftet med denna litteraturstudie var att beskriva livskvaliteten hos personer som lever med en implanterbar defibrillator (ICD), ur ett köns- och åldersperspektiv, samt att granska de inkluderade artiklarnas urvalsstrategi och undersökningsgrupp. Metod: Denna beskrivande litteraturstudies resultat sammanställdes utifrån elva granskade artiklar av kvantitativ ansats, eftersökta i databaserna PubMed och Scopus. Huvudresultat: Kvinnor rapporterade lägre livskvalitet än männen kopplat till nedsatt mental, fysisk och social funktion. Även en ökad oro och ångest förekom hos kvinnor. Psykisk ohälsa utvecklades främst hos yngre, vilka också upplevde lägre acceptans till enheten än äldre ICD- bärare vilket påverkade livskvaliteten. Äldre personer rapporterade mindre oro över att ICD:n skulle defibrillera. Dock sågs en nedsatt livskvalitet relaterat till högre förekomst av fysisk nedsättning i större grad hos äldre än yngre ICD- bärare. Fyra av resultatartiklarna angav använd urvalsstrategi. Samtliga redogjorde för antalet deltagare och hur många som var män respektive kvinnor. Åldersspannet mellan deltagarna angavs i fem artiklar. Slutsats: Olika inverkan på livskvaliteten kunde skönjas både mellan kön och olika åldrar. Dessa fynd bekräftade behovet av att sjukvårdspersonal behöver få tillgång till mer holistiska patientutbildningsprogram som inte enbart fokuserar på de tekniska aspekterna av att leva med en ICD.

Published
2015

15. Massive rearrangements of cellular MicroRNA signatures are key drivers of hepatocyte dedifferentiation

Author

Lauschke, Volker M., primary, Vorrink, Sabine U., additional, Moro, Sabrina M.L., additional, Rezayee, Fatemah, additional, Nordling, Åsa, additional, Hendriks, Delilah F.G., additional, Bell, Catherine C., additional, Sison-Young, Rowena, additional, Park, B. Kevin, additional, Goldring, Christopher E., additional, Ellis, Ewa, additional, Johansson, Inger, additional, Mkrtchian, Souren, additional, Andersson, Tommy B., additional, and Ingelman-Sundberg, Magnus, additional

Published
2016
Full Text
View/download PDF

16. Characterization of primary human hepatocyte spheroids as a model system for drug-induced liver injury, liver function and disease

Author

Bell, Catherine C., primary, Hendriks, Delilah F. G., additional, Moro, Sabrina M. L., additional, Ellis, Ewa, additional, Walsh, Joanne, additional, Renblom, Anna, additional, Fredriksson Puigvert, Lisa, additional, Dankers, Anita C. A., additional, Jacobs, Frank, additional, Snoeys, Jan, additional, Sison-Young, Rowena L., additional, Jenkins, Rosalind E., additional, Nordling, Åsa, additional, Mkrtchian, Souren, additional, Park, B. Kevin, additional, Kitteringham, Neil R., additional, Goldring, Christopher E. P., additional, Lauschke, Volker M., additional, and Ingelman-Sundberg, Magnus, additional

Published
2016
Full Text
View/download PDF

18. Amidoxime Reductase System Containing Cytochrome b5 Type B (CYB5B) and MOSC2 Is of Importance for Lipid Synthesis in Adipocyte Mitochondria

Author

Neve, Etienne P A, Nordling, Åsa, Andersson, Tommy B, Hellman, Ulf, Diczfalusy, Ulf, Johansson, Inger, Ingelman-Sundberg, Magnus, Neve, Etienne P A, Nordling, Åsa, Andersson, Tommy B, Hellman, Ulf, Diczfalusy, Ulf, Johansson, Inger, and Ingelman-Sundberg, Magnus

Abstract

Reduction of hydroxylamines and amidoximes is important for drug activation and detoxification of aromatic and heterocyclic amines. Such a reductase system was previously found to be of high activity in adipose tissue and liver, and furthermore, in vitro studies using recombinant truncated and purified enzymes suggested the participation of cytochrome b(5) reductase (CYB5R), cytochrome b(5) (CYB5), and molybdenum cofactor sulfurase C-terminal containing 1 and 2 (MOSC1 and -2). Here, we show that purified rat liver outer mitochondrial membrane contains high amidoxime reductase activity and that MOSC2 is exclusively localized to these membranes. Moreover, using the same membrane fraction, we could show direct binding of a radiolabeled benzamidoxime substrate to MOSC2. Following differentiation of murine 3T3-L1 cells into mature adipocytes, the MOSC2 levels as well as the amidoxime reductase activity were increased, indicating that the enzyme is highly regulated under lipogenic conditions. siRNA-mediated down-regulation of MOSC2 and the mitochondrial form of cytochrome b(5) type B (CYB5B) significantly inhibited the reductase activity in the differentiated adipocytes, whereas down-regulation of MOSC1, cytochrome b(5) type A (CYB5A), CYB5R1, CYB5R2, or CYB5R3 had no effect. Down-regulation of MOSC2 caused impaired lipid synthesis. These results demonstrate for the first time the direct involvement of MOSC2 and CYB5B in the amidoxime reductase activity in an intact cell system. We postulate the presence of a novel reductive enzyme system of importance for lipid synthesis that is exclusively localized to the outer mitochondrial membrane and is composed of CYB5B, MOSC2, and a third unknown component (a CYB5B reductase).

Published
2012
Full Text
View/download PDF

20. Zonation of cytochrome <em>P</em>450 isozyme expression and induction in rat liver.

Author

Bühler, Rolf, Lindros, Kai O., Nordling, Åsa, Johansson, Inger, and Ingelman-Sundberg, Magnus

Subjects
CYTOCHROMES, HEMOPROTEINS, BIOLOGICAL pigments, GENETICS, BIOCHEMISTRY, MOLECULAR biology
Abstract

The regional expression of six different cytochrome P450 (CYP) forms in rat liver under constitutive and induced conditions was compared using immunological techniques, lmmunostaining of consecutive thin sections from control liver revealed that the same hepatocytes, forming a 6-8 cells thick layer surrounding the terminal hepatic venules, were stained for CYP2B1/2, CYP2EI and CYP3A1. Staining of CYP2A1 extended further into the midzonal region, whereas all cells of the acinus stained for CYPEtOH2, These results were supported by Western blot analysis of cell lysates from the periportal or perivenous region obtained by zone-restricted digitonin treatment during in situ perfusion. The data suggest three distinct patterns of constitutive P450 expression: perivenousrestricted (CYP2BI/2, CYP2E1 and CYP3A1); pefivenous-dominated (CYP2AI) and panacinar (CYPEtOH2). Chronic exposure to ethanol caused induction of CYP2E1 in the same cells already being constitutively expressed, whereas CYPEtOH2 was more induced in the pefiportal area. The relative induction of CYP2B1/2, CYP3A1 and CYPEtOH2 after treatment with phenobarbital was stronger in periportal hepatocytes, resulting in levelling out of the initial pedvenous dominance of CYP2B1/2 and CYP3A1, whereas CYPEtOH2 became pefiportal-dominated. Acetone induced CYP2E1, CYP2Cll and CYP3A1 selectively in the perivenous area. These studies indicate that a particular P450 isozyme is generally induced in the same cells where it is constitutively expressed, and that this regional selectivity is independent of the kind of inducer. The data suggest that, during maturation, the hepatocytes acquire various phenotypes in the pefiportal and perivenous region, to respond differently to endogenous and exogenous signals in the control of P450 expression. [ABSTRACT FROM AUTHOR]

Published
1992
Full Text
View/download PDF

21. CHARACTERIZATION AND PARTIAL PURIFICATION OF THE RAT AND HUMAN ENZYME SYSTEMS ACTIVE IN THE REDUCTION OF N-HYDROXYMELAGATRAN AND BENZAMIDOXIME

Author

Andersson, Susanne, Hofmann, Yvonne, Nordling, Åsa, Li, Xue-qing, Nivelius, Sabina, Andersson, Tommy B., Ingelman-Sundberg, Magnus, and Johansson, Inger

Abstract

The enzymic basis for intracellular reduction of N-hydroxylated amidines to their corresponding amidines, and hydroxylamines to their corresponding amines, is unknown. The hydroxylated amidines can be used as prodrug moieties, and an understanding of the enzyme system active in the reduction can contribute to more efficient drug development. In this study, we examined the properties of this enzyme system using benzamidoxime and N-hydroxymelagatran as substrates. In rats and humans, the hepatic enzyme system was localized in mitochondria as well as in microsomes, using preferably NADH as cofactor. Potassium cyanide, N-methylhydroxylamine, p-hydroxymercuribenzoate, and desferrioxamine were efficient inhibitors, whereas typical cytochrome P450 (P450) inhibitors were ineffective. In rats, the highest specific activity was found in liver, adipose tissue, and kidneys, whereas in humans, the specific activity in the preparations of adipose tissue examined was lower. A sex difference was observed in rat liver, where 4-fold higher activity was seen in microsomes from female rats. No gender differences were present in any other tissue investigated. Partial purification of the hepatic system was achieved using polyethylene glycol fractionation followed by Octyl Sepharose chromatography at low detergent concentrations, whereas the enzyme was denatured after complete solubilization. The unique appearance of the enzyme activity in adipose tissue, together with the cyanide sensitivity and the failure of typical P450 inhibitors to impede the reaction, indicates that the enzyme system active in reduction of benzamidoxime and N-hydroxymelagatran formation is not of cytochrome P450 origin, but likely consists of an NADH-dependent electron transfer chain with a cyanide-sensitive protein as the terminal component.

Published
2005

Catalog

Books, media, physical & digital resources
See catalog results