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OBJECTIVES: Determine the incremental diagnostic yield of prenatal exome sequencing (pES) over chromosome microarray (CMA) or G-banding karyotype in fetuses with central nervous system (CNS) abnormalities. METHODS: Data were collected via electronic searches from January 2010 to April 2022 in MEDLINE, Cochrane, Web of Science and EMBASE. The NHS England prenatal exome cohort was also included. Incremental yield was calculated as a pooled value using a random-effects model. RESULTS: Thirty studies were included (n = 1583 cases). The incremental yield with pES for any CNS anomaly was 32% [95%CI 27%-36%; I2 = 72%]. Subgroup analysis revealed apparent incremental yields in; (a) isolated CNS anomalies; 27% [95%CI 19%-34%; I2 = 74%]; (b) single CNS anomaly; 16% [95% CI 10%-23%; I2 = 41%]; (c) more than one CNS anomaly; 31% [95% Cl 21%-40%; I2 = 56%]; and (d) the anatomical subtype with the most optimal yield was Type 1 malformation of cortical development, related to abnormal cell proliferation or apoptosis, incorporating microcephalies, megalencephalies and dysplasia; 40% (22%-57%; I2 = 68%). The commonest syndromes in isolated cases were Lissencephaly 3 and X-linked hydrocephalus. CONCLUSIONS: Prenatal exome sequencing provides a high incremental diagnostic yield in fetuses with CNS abnormalities with optimal yields in cases with multiple CNS anomalies, particularly those affecting the midline, posterior fossa and cortex.

Mentoring by the mentor teachers to the trainee teacher is an important element in the component of professional practice, namely practicum. Mentoring of mentor teachers in this study refers to the quality of guidance given by mentor teachers to trainee teachers. This aspect is seen to have an impact on the success of trainee teachers during the practicum session. However, there are past studies that state that mentor teachers do not provide guidance as required by the trainee teachers. Therefore, this study aimed to examine the quality of mentoring of mentor teachers from the perspective of trainee teachers. This study used a mixed-method approach. Quantitative study was using a questionnaire namely mentor teacher's guidance while utilizing random sampling method was used on a sample of 217 trainee teachers from the Institute of Teacher Education in the northern zone of Malaysia who have undergone the practicum session. The qualitative approach, on the hand involve semi-structured interview with two trainee teachers as the participants. This study found that the quality of guidance of mentor teachers is at a very good level. Quantitative findings are supported by qualitative findings. Four themes were identified from the interview analysis namely; excellent guidance, informative, cooperation and the needs of mentor teachers.

This paper seeks to explore the challenges faced by excellent teachers in preparing for authentic assessment in their English as a Second Language (ESL) classrooms. This qualitative case study involving six excellent teachers was based on purposive sampling. Data sources in this study used classroom observation and a series of semi-structured interviews with the excellent teachers. Thematic analysis was used to identify emerging themes from the codes gathered from the interviews and observations. Data showed that excellent teachers faced multiple challenges prior to preparing for authentic assessment. They have also been using variations of authentic assessment to help children in their learning besides accurate documentation and extensive reading from around the globe to equip themselves with the current knowledge. Since there is no clear guideline for teachers who practice authentic assessment in their classes, this study provided some insights on the preparations and the use of authentic assessment as part of their teaching and learning process.

Writing anxiety is a significant indicator that could hinder writing abilities and lead to performance inefficiency. The study aims at assessing Iraqi ESL 4th Year College of Physical Education and Sport Sciences Students' Writing Anxiety. Iraqi ESL university students encounter greater difficulties and obstacles in this area than native writers do. The current study has a descriptive design. A scale with (20) items comprises the study's constructed instrument. The sample of the present study has randomly been selected from the College of Physical Education and Sport Sciences at the university of Baghdad from the fourth-year total (300). Face validity and reliability have been calculated. The results of this investigation indicate that Iraqi university students unluckily faced real challenges and issues in this field. The conclusions and recommendations were then determined. Keywords: Writing Skill, Anxiety

Truncated distributions arise naturally in many practical situations. It’s a conditional distribution that develops when the parent distribution's domain is constrained to a smaller area. The distribution of a right truncated is one of the types of a single truncated that is restricted within a specific field and usually occurs when the specified period for the study is complete. Hence, this paper introduces Right Truncated Inverse Generalized Rayleigh Distribution (RTIGRD) with two parameters is introduced. Then, provided some properties such as; (probability density function, cumulative distribution function (CDF), survival function, hazard function, ‎rth moment, mean, variance, Moment Generating Function, Skewness, kurtosis, Median, and Mode for Right Truncated Inverse Generalized Rayleigh Distribution on [0,1].

Background: Type1 Diabetes Mellitus is a common chronic diseases among children, and associated with morbidity, mortality, and enormous healthcare expenditures. Objectives; to estimate the prevalence, incidence, and describe the epidemiological characteristics of Type 1 Diabetes Mellitus among children in Karbala governorate, Methods: A cross-sectional study was conducted among all children who attended the main DM center in Karbala Teaching Hospital for Children, Public Clinics, and Primary Health Care centers. Data was collected by structured questionnaire and biochemical and anthropometric measurements. The statistical analysis data entry was conducted using Statistical Package for Social Sciences. Results: Total number of Type 1 Diabetes Mellitus patients aged 0-15 years in Karbala in 2015 was 199; making 44.66/100 5. Fifty-four patients were newly diagnosed patients making an incidence of 12.11/100 5. There was nearly equal distribution among both sexes. Most of the cases were from urban areas and most of the patients had a Family history of Type 2 Diabetes Mellitus. Diabetic ketoacidosis was reported in 16.8% of the patients. Only 31.6% of patients had HbA1c < 7%. Half of the patients had a history of admission to the hospital for diabetes. Logistic regression analysis revealed that the only independent variables significantly correlated with poor glycemic control were lack of Self-Monitoring Blood Glucose, irregular visits to the Diabetic Center, and positive family history of diabetes. Conclusion: Only one-third of T1DM children in Karbala city had controlled blood sugar. Lack of regular blood glucose monitoring and irregular contact with health care providers were the main determinants of uncontrolled blood sugar.

Agriculture plays a key role in Indonesia’s economy as it provides notable job opportunities for the people and contributes to the country’s foreign income. The Indonesian government initialized integration into the world economy through the implementation of development plans three decades ago. It has considerably influenced all economic sectors, including agriculture. Official data on nuts production shows that this activity has followed outstanding progress since the time that the plan came into force. Hence, the current study investigates the possible impacts of globalization on nuts production in Indonesia. To do so, we applied the non-linear autoregressive distributed lag model (NARDL) to two variables of interest, namely the nuts production and the KOF globalization index for the period 1975-2019. The main findings prove the existence of a long-run equilibrium relationship between the variables of interest. Moreover, the positive shocks to the globalization index have a direct impact on nuts production. In addition, we found evidence of the significant asymmetry between the globalization index and the nuts production in Indonesia, implying the significant difference between the effect of positive and negative shocks to globalization on nuts production.

Rapid urbanisation and rising population mobility have resulted in an increase in the number of vehicles on the road, providing considerable challenges for road traffic management authorities in the form of increased congestion, accidents, and pollution. Researchers in both industry and academia have been attempting to improve the performance of present road Traffic Management Systems in light of the issues that future ''smart cities'' would face by leveraging advances in sensing, communication, and dynamic adaptive technologies. The interaction between the development of society and the development of technologies does not escape these two factors, which impact each other more profoundly each time. This research is being conducted in response to challenges in Baghdad, Iraq, such as infrastructure, supply, and equipment, which have an impact on the proper performance of work by employees and employees who operate in the business. Fifty individuals from the Iraqi city of Baghdad were hired for the study project. Following that, the communications device research was carried out. Based on the study's findings, a strategy for upgrading Baghdad's communications network using high-availability technology is given. Its execution would help the organisation. We believe that the redesign work provided in this work can also help to improve organisational communicative performance by providing subsidies for network communications and contemplation on the most appropriate internal communication forms for public profile and organisational culture.

Background: Type1 Diabetes Mellitus is a common chronic diseases among children, and associated with morbidity, mortality, and enormous healthcare expenditures. Objectives; to estimate the prevalence, incidence, and describe the epidemiological characteristics of Type 1 Diabetes Mellitus among children in Karbala governorate, Methods: A cross-sectional study was conducted among all children who attended the main DM center in Karbala Teaching Hospital for Children, Public Clinics, and Primary Health Care centers. Data was collected by structured questionnaire and biochemical and anthropometric measurements. The statistical analysis data entry was conducted using Statistical Package for Social Sciences. Results: Total number of Type 1 Diabetes Mellitus patients aged 0-15 years in Karbala in 2015 was 199; making 44.66/1005. Fifty-four patients were newly diagnosed patients making an incidence of 12.11/1005. There was nearly equal distribution among both sexes. Most of the cases were from urban areas and most of the patients had a Family history of Type 2 Diabetes Mellitus. Diabetic ketoacidosis was reported in 16.8% of the patients. Only 31.6% of patients had HbA1c < 7%. Half of the patients had a history of admission to the hospital for diabetes. Logistic regression analysis revealed that the only independent variables significantly correlated with poor glycemic control were lack of Self-Monitoring Blood Glucose, irregular visits to the Diabetic Center, and positive family history of diabetes. Conclusion: Only one-third of T1DM children in Karbala city had controlled blood sugar. Lack of regular blood glucose monitoring and irregular contact with health care providers were the main determinants of uncontrolled blood sugar.

In most developed countries, the medical healthcare system is experiencing rapid development from the stage of clinical information to the stage of information dissemination. In all of these countries, it is undeniable that the Internet of Medical Things (IoMT) technologies have contributed in order to develop information medical healthcare. In reality, the development of smart medical healthcare has been hindered by the protection of medical privacy, according to research and acceptance. This is especially true as telecommunications systems continue to expand and wireless sensor networks (WSN) develop, as well as ways to penetrate those checks that have become increasingly difficult. In the smart healthcare system, protecting users' information remains an outstanding issue. IoMT features and the protection of privacy and security have led to the development of an extended privacy homomorphism algorithm based on scrambling matrixes, an encryption algorithm enhanced by Modified RSA (mRSA), and a method of encrypted data compression that ensures data confidentiality. For the above purpose, we built a prototype system on a demo temporary domain using both hardware and software. According to the results, the proposed scheme protects E-healthcare from potential threats by providing stakeholders with a secure interface and preventing unauthorized users from accessing the mCloud, thus ensuring privacy. E-healthcare services based on cloud technology are protected by the proposed scheme because it is simple and robust. [ABSTRACT FROM AUTHOR]

This study aimed at discussing the benefits of portfolio assessment in assessing students' writing skills. The study explores the use of authentic assessment in the classroom. Eleven primary school children from Year 4 in a rural school in Sabah participated in this study. Data were collected by observing them during the English Language lessons and at the end of the series of observations, an interview session was conducted with each of them for triangulation. Through the observation, the children can be categorized into 4 groups of improvement, which are "emerging", "beginning", "developing" and "expanding" using the coding from British Columbia (1999). The results of this study revealed that using portfolio to assess young learners' writing skills is beneficial and effective in helping them to write in the English language classroom. The value of this study is to suggest the use of a more authentic classroom assessment, particularly in English language teaching and learning.

The research deals with the study of the varying coefficient model as well as the Time-Varying Coefficient Model because of their remarkable interest in recent years. Methods Two methods were used, local polynomial kernal method and the cubic spline method. The simulation method was used for comparison. Compared to three mathematical functions that represented the variable coefficients, different sample sizes, and different levels of the model's standard deviation, the cubic spline method was best.

Background: Chronic myeloid leukemia (CML) is a clonal myeloproliferative neoplasm that is characterized by the presence of the Philadelphia (Ph) chromosome, which results from a reciprocal translocation between the breakpoint cluster region (BCR) of chromosome 22 and the Abelson homolog 1 (ABL1) region of chromosome 9. Programmed death-ligand 1 (PD-L1; also known as B7 homolog 1 and CD274) is an immune checkpoint protein, and its engagement with programmed cell death protein-1 (PD-1) receptor on T cells activates co-inhibitory signaling to suppress the function of cytotoxic T lymphocytes (CTLs). Interleukin-2 receptor (IL-2R, CD25) expression is a soluble form of the a-chain of IL-2R (sIL-2R). The IL-2/IL-2 receptor α (IL-2RA) signaling pathway is essential for the regulation of immune responses. Objective: To assess the plasma level of PDL-1 and CD25 in patients with CML and their correlations with response to the first line of therapy, tyrosine kinase inhibitor (imatinib). Materials and Methods: This case-control study was conducted on 66 patients with CML in the chronic phase (CP) from May 2019 to October 2019. The patients were sequentially selected and subdivided into three subgroups: 20 new diagnoses before starting treatment, 30 imatinib therapy responders, and 16 imatinib resistant for each group. Plasma samples were tested for the levels of PD-L1 and CD25 by the enzyme-linked immunosorbent assay technique. Results: Programmed cell death ligands were significantly increased in the plasma of patients with CML compared with the control group, and there was a significant increase in the plasma PD-L1 of patients with a new diagnosis compared with imatinib responders and patients who were resistant. There was no significant difference in PD-L1 level between the responder and resistant groups. Soluble CD25 was significantly increased in the plasma of patients with CML compared with the control group, and there was a significant increase in the plasma CD25 of patients with a new diagnosis compared with imatinib responders and patients who were resistant. There was no significant difference in CD25 level between responder and resistant groups. Conclusion: Plasma PD-L1 and CD25 levels were significantly higher in adult patients with CML compared with healthy subjects, and these levels were significantly higher in patients with CML who are newly diagnosed compared with imatinib therapy responders and resistant counterparts. There is no significant difference between responders and resistant groups in regard to PD-L1 and CD25.

[18F]sodium fluoride ([18F]NaF) is recognised to be superior to [99mTc]-methyl diphosphate ([99mTc]Tc-MDP) and 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) in bone imaging. However, there is concern that [18F]NaF uptake is not cancer-specific, leading to a higher number of false-positive interpretations. Therefore, in this work, [18F]AlF-NOTA-pamidronic acid was prepared, optimised, and tested for its in vitro uptake. NOTA-pamidronic acid was prepared by an N-Hydroxysuccinimide (NHS) ester strategy and validated by liquid chromatography-mass spectrometry analysis (LC-MS/MS). Radiolabeling of [18F]AlF-NOTA-pamidronic acid was optimised, and it was ensured that all quality control analysis requirements for the radiopharmaceuticals were met prior to the in vitro cell uptake studies. NOTA-pamidronic acid was successfully prepared and radiolabeled with 18F. The radiolabel was prepared in a 1:1 molar ratio of aluminium chloride (AlCl3) to NOTA-pamidronic acid and heated at 100 °C for 15 min in the presence of 50% ethanol (v/v), which proved to be optimal. The preliminary in vitro results of the binding of the hydroxyapatite showed that [18F]AlF-NOTA-pamidronic acid was as sensitive as [18F]sodium fluoride ([18F]NaF). Normal human osteoblast cell lines (hFOB 1.19) and human osteosarcoma cell lines (Saos-2) were used for the in vitro cellular uptake studies. It was found that [18F]NaF was higher in both cell lines, but [18F]AlF-NOTA-pamidronic acid showed promising cellular uptake in Saos-2. The preliminary results suggest that further preclinical studies of [18F]AlF-NOTA-pamidronic acid are needed before it is transferred to clinical research.

Rare copy number variants (CNVs) disrupting ASTN2 or both ASTN2 and TRIM32 have been reported at 9q33.1 by genome-wide studies in a few individuals with neurodevelopmental disorders (NDDs). The vertebrate-specific astrotactins, ASTN2 and its paralog ASTN1, have key roles in glial-guided neuronal migration during brain development. To determine the prevalence of astrotactin mutations and delineate their associated phenotypic spectrum, we screened ASTN2/TRIM32 and ASTN1 (1q25.2) for exonic CNVs in clinical microarray data from 89 985 individuals across 10 sites, including 64 114 NDD subjects. In this clinical dataset, we identified 46 deletions and 12 duplications affecting ASTN2. Deletions of ASTN1 were much rarer. Deletions near the 3' terminus of ASTN2, which would disrupt all transcript isoforms (a subset of these deletions also included TRIM32), were significantly enriched in the NDD subjects (P = 0.002) compared with 44 085 population-based controls. Frequent phenotypes observed in individuals with such deletions include autism spectrum disorder (ASD), attention deficit hyperactivity disorder (ADHD), speech delay, anxiety and obsessive compulsive disorder (OCD). The 3'-terminal ASTN2 deletions were significantly enriched compared with controls in males with NDDs, but not in females. Upon quantifying ASTN2 human brain RNA, we observed shorter isoforms expressed from an alternative transcription start site of recent evolutionary origin near the 3' end. Spatiotemporal expression profiling in the human brain revealed consistently high ASTN1 expression while ASTN2 expression peaked in the early embryonic neocortex and postnatal cerebellar cortex. Our findings shed new light on the role of the astrotactins in psychopathology and their interplay in human neurodevelopment.

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Objective: Chromosomal microarray (CMA), while considered the gold standard for detecting copy number variants (CNVs) in prenatal diagnostics, has its limitations, including the necessity to replace aging microarray equipment, low throughput, a static design, and an inefficient multi‐day workflow. This study evaluates the feasibility of low‐pass genome sequencing (LP‐GS) as a potential replacement for CMA in prenatal diagnostics. Methods: We comprehensively compared LP‐GS at 10x and 5x average depths with CMA in a prenatal laboratory. We examined parameters, including concordance, sensitivity, specificity, workflow efficiency, and cost‐effectiveness. Results: We found a high degree of agreement between LP‐GS and CMA for detecting CNVs and absence of heterozygosity. Furthermore, compared to CMA, LP‐GS increased workflow efficiency and proved to be cost‐neutral at 10x and cost‐effective at 5x. Conclusion: Our study suggests that LP‐GS is a promising alternative to CMA in prenatal diagnostics, offering advantages, including a more efficient workflow and scalability for larger testing volumes. Importantly, for clinical laboratories that have adopted next‐generation sequencing in a separate capacity, LP‐GS facilitates a unified NGS‐centric approach, enabling workflow consolidation. By offering a single, streamlined platform for detecting a broad range of genetic variants, LP‐GS may represent a critical step toward enhancing the diagnostic capabilities of prenatal laboratories. Key points: What's already known about this topic? Chromosomal microarray (CMA) is currently the gold standard for analyzing copy number variants (CNVs) in prenatal diagnostics.However, CMA is limited by relatively low throughput, inefficient workflow, and a static test design that does not easily accommodate updates or novel disease associations.With more laboratories transitioning to next‐generation sequencing (NGS), there is a growing need for a unified workflow to streamline diagnostic procedures.Low‐pass genome sequencing (LP‐GS) is emerging as an alternative to CMA for CNV detection. What does this study add? Our study assessed the potential of LP‐GS to replace CMA in our prenatal laboratory setting.We systematically compared the performance of LP‐GS at 10x and 5x average depths to CMA and found excellent concordance, sensitivity, and specificity.LP‐GS has a streamlined and efficient workflow compared to CMA and offers economic benefits of being cost‐neutral at 10x and cost‐effective at 5x average read depths.Our findings indicate that LP‐GS is a potential alternative to CMA in prenatal diagnostics addressing some of the existing constraints in the field, including the shortage of genetic technologists. [ABSTRACT FROM AUTHOR]

Currently, the available indices to measure mangrove health are not comprehensive. An integrative ecological-socio economic index could give a better picture of the mangrove ecosystem health. This method explored all key biological, hydrological, ecological and socio-economic variables to form a comprehensive mangrove quality index. A total of 10 out of 43 variables were selected based on principal component analysis (PCA). They are aboveground biomass, crab abundance, soil carbon, soil nitrogen, number of phytoplankton species, number of diatom species, dissolved oxygen, turbidity, education level and fishing time spent by fishers. Two types of indices were successfully developed to indicate the health status viz., (1) Mangrove quality index for a specific category (MQISi) and, (2) Overall mangrove quality index (MQI) to reflect the overall health status of the ecosystem. The indices for the five different categories were mangrove biotic integrity index (MQIS1), mangrove soil index (MQIS2), marine-mangrove index (MQIS3), mangrove-hydrology index (MQIS4) and mangrove socio-economic index (MQIS5). The quality of the mangroves was classified from 1 to 5 viz. 1 (worst), 2 (bad), 3 (moderate), 4 (good), 5 (excellent). These MQI class could reflect the quality of mangrove forest which could be managed with the objective of improving its quality. Advantages of this method include: • PCA to select metrics from ecological-socioeconomic variables • Formulation of MQI based on selected metrics • Comprehensive index to classify mangrove ecosystem health Method name: How to develop a comprehensive mangrove quality index?, Keywords: Mangrove health, PCA, Indicator, Formulation

The reactive yellow azo dye (λmax = 420 nm) is widely utilized for textile coloring due to its low-cost stability and tolerance properties. Treatment of dye-containing wastewater by traditional methods is usually inadequate because of its resistance to biological and chemical degradation. From this research, the continuous reactor of an advanced oxidation method supported the use of H2O2/TiO2/UV to remove the coloration of the reactive yellow dye from the discharge. At constant best conditions obtained from the batch reactor tests pH=7, H2O2 dosage = 400 mg/l and TiO2=25mg/l , the aqueous solutions were tested in the continuous reactor at different dye concentration and different flow rates . The maximum removal efficiency was found to be 91.552% obtained at the flow rate 5 l/h, also the results of decomposition information proved that method was pseudo-first-order kinetics.

Although autism spectrum disorders (ASDs) have a substantial genetic basis, most of the known genetic risk has been traced to rare variants, principally copy number variants (CNVs). To identify common risk variation, the Autism Genome Project (AGP) Consortium genotyped 1558 rigorously defined ASD families for 1 million single-nucleotide polymorphisms (SNPs) and analyzed these SNP genotypes for association with ASD. In one of four primary association analyses, the association signal for marker rs4141463, located within MACROD2, crossed the genome-wide association significance threshold of P < 5 × 10(-8). When a smaller replication sample was analyzed, the risk allele at rs4141463 was again over-transmitted; yet, consistent with the winner's curse, its effect size in the replication sample was much smaller; and, for the combined samples, the association signal barely fell below the P < 5 × 10(-8) threshold. Exploratory analyses of phenotypic subtypes yielded no significant associations after correction for multiple testing. They did, however, yield strong signals within several genes, KIAA0564, PLD5, POU6F2, ST8SIA2 and TAF1C.

The ball and beam is, in fact, a standout amongst the essentially vital models, which are generally utilized for educating the control system because its simplicity to be built, modeled, and controlled. This system involve a ball roll on a beam and since the angle of the beam manipulate by servo motor, so the purposes of this paper is to design two stages controller to stabilize the ball position along the beam by varying the angle of beam. The First controller stage “is Proportional- Integral- Derivative” (PID) “for servo motor. The second stage of the controller is Proportional-Derivative (PD) to control the ball and beam plant. The Matlab simulation results illustrate the efficiency of this controller. Furthermore, this paper presents the complete hardware Field Programmable Gate Arrays (FPGA) design with real time implementation for the suggested controller. This controller utilized 1% of occupied slices when implement on Spartan-3A DSP 3400A Xilinx kit with 70.265 𝑛𝑠 minimum time required to complete the controller function. The experimental tests shows that the suggested two stages controller satisfy the functional requirements results.