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The aim of this study was to evaluate the effect of genetic groups and diets with different sources of nonprotein nitrogen (NPN) on the carcass and meat characteristics of beef heifers. The meat from 40 heifers (20 ½ Angus ½ Nellore (A × N) and 20 ½ Charolais ½ Nellore (L × N)), finished in feedlots, was used. The heifers were fed diets containing different sources of NPN-(1) a diet with livestock urea and protected urea (LPU) and (2) a diet with extruded urea (EU)-in a completely randomized design with a 2 × 2 factorial arrangement. Carcass, composition and meat quality evaluations were carried out. There were no significant interactions between diet and genetic group for most of the variables evaluated ( p > 0.05). The A × N heifers had higher hot carcass weights (305.73 vs. 279.80 kg), loin eye areas (80.87 vs. 75.45 cm 2 ), subcutaneous fat thicknesses (8.69 vs. 6.35 mm) and lower shear forces (6.98 vs. 7.7 kg) compared to the C × N heifers ( p < 0.05). The meat from the A × N heifers had higher proportions of saturated fatty acids (49.41 vs. 47.95%), with no effects on the proportions of monounsaturated (47.57%) and polyunsaturated (4.01%) fatty acids. The A × N heifers had better carcass and meat characteristics, while the C × N heifers had meat and fat with better fatty acid profiles.

Our objectives were to evaluate the use of cottonseed cake in replacing corn silage in a diet without forage and to identify the model with higher precision and accuracy of adjustment of parameters of ruminal degradation kinetics. A diet containing corn silage and another with cottonseed cake as a fiber source were formulated. Gompertz, Dual-pool Logistic, Brody, and Ørskov models were evaluated for goodness of fit to gas production. There were significant differences in dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF) in the in vitro digestibility for diets and fiber sources. The estimated values of the Gompertz (6.77), Brody (6.72), and Ørskov (6.73) models were similar to the observed mean of gas production in the corn silage diet (6.73 mL/100 mg DM). Similarly, the estimated values of the Brody (5.87) and Ørskov (5.89) models were similar to the observed mean of gas production in the cottonseed cake diet (5.87 mL/100 mg DM). The roughage-free diet containing cottonseed cake as a fiber source stimulated higher gas production. Brody and Ørskov models presented higher precision and accuracy in the fitting of kinetics of degradation independent of the fiber source in the diet.

We hypothesized that the protein source in supplements and the insolation and ambient temperature changes in different seasons could cause changes in the skin of Nellore steers during the rearing phase on warm-climate pasture. The objective of this study was to evaluate the effects of replacing true protein (soybean meal) with NPN (extruded urea) in the supplement on the skin characteristics of steers grazing on Marandu grass pastures. Thirty-six Nellore steers with an average initial weight of 250.0 kg and 15 months of age were used. Skin biopsies were performed at three different times: summer, autumn, and winter. The protein source has effects on the length of the glandular portion, number of follicles, and gland area in steers during the rearing phase at different seasons of the year. In the summer, the skin presented a higher compact structure, while in the autumn and winter, the skin presented a sparser arrangement. Skin from steers that received soybean meal in supplement had a more significant number of follicles in the summer and a smaller area and length of sweat glands. The dermis thickness of steers supplemented with soybean meal was greater than that of urea supplemented. The epidermis thickness and dermis of the steers' skin were greater at the autumn (April). Sweat gland depth was greater in autumn (April) and winter (July) than in summer (December). The protein source in the supplement alters the skin characteristics by increasing of dermis thickness of Nellore steers during the growth phase. The insolation and ambient temperature changes in different seasons alter the skin structure by increasing the epidermis and dermis thickness, sweat glands depth, and glands area of the skin of steers during the growth phase., (© 2023. The Author(s).)

It aimed to evaluate the effects of different whole oilseeds in lipid-rich diets on nutrient intake, apparent digestibility, ingestive behavior, and ruminal and blood parameters of steers. A control diet (without oilseed) and four diets containing whole oilseeds (cotton, canola, sunflower, and soybean) were tested. All diets used the whole-plant corn silage at 400 g/kg as roughage. Five diets, being a control diet (without oilseed) and four diets containing whole oilseeds (cotton, canola, sunflower, and soybean), were tested. All diets used the whole-plant corn silage at 400 g/kg as roughage. Five rumen fistulated crossbreed steers, in a 5 × 5 Latin square design were distributed using five periods of 21 days. The steers fed cottonseed and canola diets had lower dry matter intakes (6.6 kg/day). Steers showed higher averages of time in rumination for treatments with sunflower, soybean, and cottonseed (406, 362, and 361 min/day, respectively). There was no treatment effect for the ruminal pH and ammonia (NH 3 ) variables. There was an effect of the treatment on the volatile fatty acid concentrations. The animals that received soybean showed a higher plasma urea concentration (50.7 mg/dL). Animals fed the control diet showed lower serum cholesterol levels (111.8 mg/dL) than those fed diets containing whole cottonseed, canola, sunflower, and soybean (152.7, 137.1, 146.9, and 138.2 mg/dL, respectively). We recommended using whole soybean or sunflower seeds to formulate lipid-rich diets with 70 g/kg of ether extract for crossbreed steers in the feedlot., (© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)

The objective was to evaluate the effect of using prebiotics (Saccharomyces cerevisiae boulardii) or Monensin in the confinement initial phase and replacing monensin with probiotics (Bacillus toyonensis) in the final phase. Forty-eight Nellore steers were used, with an initial mean body weight of 356.2 ± 17.98 kg, distributed in a completely randomized design. Two animals per pen were confined in 80 m 2 pens. The experiment was divided into two stages. The first phase lasted from day 1 to the 30th day, during which the animals were divided into two groups of 24 animals each. The treatments were the nutritional additives added to the diet: monensin or prebiotics (Saccharomyces cerevisiae boulardii). In the second phase, each group was subdivided into 12 animals by treatment, which received monensin or probiotics (Bacillus toyonensis). Dry matter intake (DMI), animal performance, and economic evaluation of the use of additives were evaluated. There was no additive effect on DMI, average daily gain, and total weight gain of the animals in the first experimental stage (0-30th day). Likewise, in the second stage (31st-100th day), there was no treatment effect for the variables of intake and performance. There was no effect of the use of different nutritional additives on carcass characteristics. The use of prebiotics sequentially to probiotics promoted gross and net yield that was superior to that of the animals that consumed monensin. Yeasts and bacteria respectively in the first and second phases of confinement can replace monensin in confinement diets., (© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)

Protodioscin poisoning of Brachiaria spp. has been a serious problem for lambs in grazing systems. The defoliation process can stimulate the appearance of new leaves and, the younger leaves have a lower concentration of protodioscin. Thus, it was aimed to assess the effect of different leaf offerings on the protodioscin content in forage and if protodioscin can interfere with the dry matter intake (DMI) and on metabolic and productive parameters of lambs. Twelve tester lambs (average weight 17.5 ± 3.48 kg) were divided into four groups of different levels of dry leafy matter (60, 75, 90, or 105 g/kg body weight). In addition to these, 33 regulator lambs were used as needed to adjust the leaf offerings of pasture. The animals were divided and kept in 12 paddocks, each with a tester lamb. The DMI was estimated using chromic oxide as an external marker. In vitro digestibility and degradation kinetics and ruminal, blood, and urinary parameters were measured in the forage sampled by the hand plucking method. The protodioscin concentration in forage was determined by high-performance liquid chromatography. The DMI increased linearly as a result of the supply of leaves. However, the protodioscin content and its consumption showed a quadratic behavior. There was no effect of leaf supply on in vitro digestibility, in vitro degradation kinetics of forage, and on ruminal, blood, and urinary parameters of lambs. However, a negative correlation was observed between the DMI and the concentration of protodioscin at the highest level of leaf supply. This is due to the fact that in the most intense grazing, there is a stimulus for greater regrowth; therefore, there was an increase in the concentration of protodioscin in forages in older forages. Protodioscin poisoning was confirmed by urinary and blood parameters.