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41 results on '"Nonaka, Yasuhiro"'

15. Crystal structure and conformational stability of a galectin-1 tandem-repeat mutant with a short linker.

Author

Nonaka, Yasuhiro, Ogawa, Takashi, Shoji, Hiroki, Nishi, Nozomu, Kamitori, Shigehiro, and Nakamura, Takanori

Subjects
*STRUCTURAL stability, *CRYSTAL structure, *TERTIARY structure, *PEPTIDES, *GALECTINS, *TANDEM repeats
Abstract

Modification of the domain architecture of galectins has been attempted to analyze their biological functions and to develop medical applications. Several types of galectin-1 repeat mutants were previously reported but, however, it was not clear whether the native structure of the wild type was retained. In this study, we determined the crystal structure of a galectin-1 tandem-repeat mutant with a short linker peptide, and compared the unfolding profiles of the wild type and mutant by chemical denaturation. The structure of the mutant was consistent with that of the dimer of the wild type, and both carbohydrate-binding sites were retained. The unfolding curve of the wild type with lactose suggested that the dimer dissociation and the tertiary structure unfolding was concomitant at micromolar protein concentrations. The midpoint denaturant concentration of the wild type was dependent on the protein concentration and lower than that of the mutant. Linking the two subunits significantly stabilized the tertiary structure. The mutant exhibited higher T-cell growth-inhibition activity and comparable hemagglutinating activity. Structural stabilization may prevent the oxidation of the internal cysteine residue. [ABSTRACT FROM AUTHOR]

Published
2022
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17. STPR, a 23-amino acid tandem repeat domain, found in the human function-unknown protein ZNF821

Author

Nonaka, Yasuhiro, Muto, Hideki, Aizawa, Tomoyasu, Okabe, Etsuro, Myoba, Shohei, Yokoyama, Takuya, Saito, Shin, Tatami, Fumie, Kumaki, Yasuhiro, Kamiya, Masakatsu, Kikukawa, Takashi, Mizuguchi, Mineyuki, Takiya, Shigeharu, Kinjo, Masataka, Demura, Makoto, and Kawano, Keiichi

Subjects
Silkworms -- Genetic aspects, Silkworms -- Physiological aspects, Proteins -- Structure, Proteins -- Analysis, DNA-ligand interactions -- Analysis, Peptides -- Structure, Peptides -- Chemical properties, Biological sciences, Chemistry
Abstract

The peptides of silkworm FMBP-1 STPR (sSTPR), a 23-amino acid tandem repeat domain, and human ZNF821 STPR (hSTPR) are prepared and their DNA binding behaviors are compared. STPR sequences are found in many proteins from vertebrates, insects and nematodes and some of the consensus amino acid residues is responsible for DNA binding and concomitant has increased in [alpha]-helix structure content.

Published
2010

18. Novel pyromellitic diimide-based macrocycle with a linear [pi]-electronic system and bis(phenylethynyl)pyromellitic diimide: syntheses, structures, photophysical properties, and redox characteristics

Author

Kato, Shin-ichiro, Nonaka, Yasuhiro, Shimasaki, Toshiaki, Goto, Kenta, and Shinmyozu, Teruo

Subjects
Clathrate compounds -- Chemical properties, Organometallic compounds -- Structure, Organometallic compounds -- Chemical properties, Oxidation-reduction reaction -- Analysis, X-ray crystallography -- Usage, Biological sciences, Chemistry
Abstract

The syntheses, structures, photophysical properties, and redox characteristics of the [2+2] pyromellitic diimide-based macrocycle with a linear [pi]-electronic system as well as the 3,6-bis(phenylethynyl)pyromellitic diimide derivatives are reported. The results showed that the 3,6-bis(phenylethynyl)pyromellitic diimide moiety with elongated [pi]-system, aggregation ability, and electronic affinity as a new n-type structural motif can be used for preparation of organic field-effect transistor (OFET) materials and creation of pyromellitic diimide-based [pi]-electron materials.

Published
2008

20. Galectin-9 induces atypical ubiquitination leading to cell death in PC-3 prostate cancer cells.

Author

Itoh, Aiko, Nonaka, Yasuhiro, Ogawa, Takashi, Nakamura, Takanori, and Nishi, Nozomu

Subjects
*GALECTINS, *UBIQUITINATION, *PROSTATE cancer, *CELL death, *DNA analysis, *ENDOCYTOSIS
Abstract

Galectin-9 is the most potent inducer of cell death in lymphomas and other malignant cell types among the members of the galectin family. We investigated the mechanism of galectin-9-induced cell death in PC-3 prostate cancer cells in comparison with in Jurkat T cells. Galectin-9 induced apoptotic cell death in Jurkat cells, as typically revealed by DNA ladder formation. On the other hand, DNA ladder formation and other features of apoptosis were not apparent in PC-3 cells undergoing galectin-9-induced death. Exogenous galectin-9 was endocytosed and destined to the lysosomal compartment in PC-3 cells. The internalized galectin-9 was resistant to detergent solubilization but was solubilized with lactose. Agents inhibiting actin filament dynamics abolished the internalization and cytocidal effect of galectin-9 in PC-3 but not Jurkat cells. Galectin-9 induced accumulation of ubiquitinated proteins, possibly heterogeneously ubiquitinated and/or monoubiquitinated proteins, in PC-3 cells. PYR-41, an inhibitor of the ubiquitin-activating E1 enzyme, suppressed the cytocidal effect of galectin-9. Although ubiquitination was upregulated also in Jurkat cells by galectin-9, PYR-41 was ineffective against galectin-9-induced cell death. Colocalization of ubiquitinated proteins and LAMP-1 was detectable in PC-3 cells treated with galectin-9. The ubiquitinated proteins were recovered in the insoluble fraction upon cell fractionation. In contrast, ubiquitinated proteins that accumulated after treatment with proteasome inhibitors did not co-localize with LAMP-1 and were mainly recovered in soluble fraction. The results suggest that atypical ubiquitination and accumulation of ubiquitinated proteins in lysosomes play a pivotal role in galectin-9-induced non-apoptotic death in PC-3 cells. [ABSTRACT FROM AUTHOR]

Published
2019
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24. 1P083 STPR, a 23-amino-acid tandem repeat domain, found in the human function-unknown protein ZNF821(Protein:Function,The 48th Annual Meeting of the Biophysical Society of Japan)

Author

OKABE, Etsuro, primary, NONAKA, Yasuhiro, additional, MUTO, Hideki, additional, AIZAWA, Tomoyasu, additional, MYOBA, Shohei, additional, KAMIYA, Masakatsu, additional, KIKUKAWA, Takashi, additional, TAKIYA, Shigeharu, additional, KINJO, Masataka, additional, DEMURA, Makoto, additional, and KAWANO, Keiichi, additional

Published
2010
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27. 1TP5-09 DNA-binding function of the score and three amino acid peptide repeat domain in a human unknown protein(The 47th Annual Meeting of the Biophysical Society of Japan)

Author

Nonaka, Yasuhiro, primary, Myoba, Shohei, additional, Okabe, Etsuro, additional, Muto, Hideki, additional, Kamiya, Masakatsu, additional, Kikukawa, Takashi, additional, Aizawa, Tomoyasu, additional, Kinjo, Masataka, additional, Takiya, Shigeharu, additional, Demura, Makoto, additional, and Kawano, Keiichi, additional

Published
2009
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35. Spontaneous Dislocation of the Arytenoid Cartilage

Author

Saigusa, Hideto, primary, Nonaka, Yasuhiro, additional, Ikezono, Tetsuro, additional, Aino, Iichiro, additional, Iwasaki, Chiharu, additional, Kokawa, Takayuki, additional, Nakamura, Takashi, additional, and Yagi, Toshiaki, additional

Published
2005
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