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The runout behavior of rockfall is mostly determined by the terrain and its mechanical parameters. The two factors can be significantly changed in a short period of time due to rockfall deposits in high-frequency rockfall regions. In this paper, a self-evolution process of rockfall from the Hongshiyan post-earthquake rock slope is observed and analyzed. This self-evolution process of the rockfall is the consequence of the re-occurrences of a large number of rockfall events in a short period of time, which lead to an increase in the elevation, slope angle, and hardness of the terrain, and aggravate further the severity of subsequent rockfall disasters. In order to analyze the self-evolution process, numerical simulations based on a probabilistic model were carried out on eight different conditions to analyze the interaction between the rockfall and different ground surface. The corresponding rockfall hazard was then analyzed according to a quantitative assessment method. The statistics of the average class (AC) and mitigation index (MI) of the rockfall were proposed to quantify the rockfall characteristics in different conditions. The effectiveness of structural and non-structural rockfall protection measures in high-frequency rockfall areas was discussed. An interim non-structural measure that addresses the self-evolution of rockfall is proposed to improve the mitigation efficiency, which can also reduce the maintenance cost. [ABSTRACT FROM AUTHOR]

The major damage observed in recent earthquakes is the separation of non-structural walls from structural components and large longitudinal and diagonal cracks in them; so, selecting the appropriate wall is very important. Considering the diversity of the existing walls and the multiplicity of the criteria, making an inclusive decision for choosing the most practical wall which considers all the relevant criteria seems necessary. Therefore, Analytical Hierarchy Process method was used for analysis in this paper. This method is compatible with multiple criteria and decision making purposes. In order to identify and determine the importance of effective criteria in choosing the appropriate wall, the Empirical comparisons have been used and for a more accurate comparison between the types of walls based on quantitative criteria, in addition to the questionnaire, numerical modeling has also been used. criteria include design criterion, performance criterion, economic criterion, and time criterion. Finally, using the hierarchical analysis method, the suitable types of walls in seismic areas have been prioritized. The materials used to evaluate the non-loaded walls are: 3D-panels, drywalls, cement blocks containing lightweight expanded clay aggregate (lECA), Autoclaved aerated concrete. The gained results of the study indicate that in order to achieve a safe and earthquake-resistant hospital structure, the drywalls is considered to be the best option and autoclaved aerated concrete as well as blocks options the light expanded clay aggregate and 3D-panel were selected respectively.

Innovative damage-mitigation technologies have been recently developed to improve the seismic performance of structural and non-structural elements. The combination of these solutions can lead to a high-performance and cost-efficient building system, capable of sustaining earthquakes with limited damage and reduced socio-economic losses. This article investigates the convenience of implementing damage-control solutions through a cost/performance-based evaluation of multi-story-reinforced concrete buildings, comprising alternative combinations of traditional vs low-damage technologies for both structural skeletons (frames, walls) and non-structural elements (heavy/light facades, heavy/light partitions, suspended ceilings). The significant benefits of the innovative systems are investigated through loss assessment studies, implemented using a practical approach based on numerical pushover analyses and the capacity spectrum method. The parametric analyses confirm that the integrated low-damage structural/non-structural system can lead to significant savings, in these specific cases, in the range of 150–300 €/m2 during the 50-year building-life and downtime reductions at ultimate limit state in the order of 2–7 months. [ABSTRACT FROM AUTHOR]

Multi-target searching is a hotspot and foundation topic in multi-agent systems research. However, most of the research is based on simple environment or known environment, which greatly limits the application of target search. In the non-structured environment, the searching result can be greatly affected by the complex terrain constraints and detectability of targets especially when we have no prior knowledge about the environment. In the paper, a novel search strategy combining maximum visibility and particle swarm optimization is proposed for the target search problem in a completely unknown and non-structural environment. The strategy utilizes the concept of visibility to describe how well the agent detects the map, and guides the agent to perform online path planning to complete the search task. In addition, considering the limited communication distance and communication bandwidth, the strategy introduces a cooperative mechanism for each agent to improve the search efficiency. Finally, in the experimental part, the search strategy is compared with the commonly used search strategies. Compared with the methods combining advantages, the proposed strategy can still achieve similar results, which proves the feasibility and efficiency of the strategy. [ABSTRACT FROM AUTHOR]

The evidence base for the performance and effectiveness of non-structural measures to manage stormwater pollution in industrial areas is relatively underdeveloped, despite their increased use in practice. This study aims to advance stormwater management practice and research by presenting a detailed case study of the development, implementation and evaluation of a targeted behaviour change trial that engaged small to medium industrial businesses in stormwater pollution prevention. Utilising a combination of different behaviour change strategies - including capacity building, social norms and commitment - a number of preventative stormwater pollution behaviours were changed in participating businesses. Our study provides a practice model for tackling stormwater pollution from a behavioural perspective that can be further developed by both practitioners and researchers to create effective and long-lasting change. [ABSTRACT FROM AUTHOR]

A numerical study of unobstructed content sliding within several low-to-midrise reinforced concrete cantilever wall buildings designed to Wellington conditions in New Zealand is performed to validate the belief that increasing a building's strength and/or stiffness would result in more severe sliding response. It was shown that contents within stronger buildings experienced larger sliding response. If the building was designed to be strong, the sliding response of contents with a friction coefficient of 0.1 was smaller in stiffer buildings compared with those in flexible buildings. However, the trends start reversing with an increase in friction coefficient or a decrease in building strength. Overall, content sliding is not necessarily more severe in stiffer buildings, and in many cases, the opposite is true. This study's findings were compared against an existing parametric equation for estimating the maximum sliding displacement. This equation, which was originally derived for contents located within elastic frame buildings, was found to be more efficient than considering total floor accelerations alone but was underconservative by a mean of 17% for yielding multistorey buildings. A design procedure considering content sliding using the parametric equation and an example are provided. Copyright © 2016 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Flood resilience and sustainable development in urban Nigeria: integrating traditional and non-structural methods of mitigating and adapting to flooding in cross river state, south-eastern Nigeria. We examined application of non-structural measures in addition to conventional structural approaches by Government Agency and community for flood management in Cross River State (Nigeria) at: regional-ambit and community levels. We used focus group discussion in depth interview, and observation methods to collect data from primary and secondary sources. Our findings include: emphasis on structural flood control measures by government agencies contrasted to use of rudimentary non-structural approaches by communities. Conceptual frames proposed for managing disasters include: emphasizing future climate change impacts based on multiple scales (temporal, spatial and societal) and emphasizing historical response to disasters without increasing the visibility of climate change. We conclude that community institutions, non-government/civil society organizations should lead public institutions in promoting flood resilience based on integrated non-structural to structural measures and show recent developments regarding civil society coalition committed towards promoting environmental governance in Nigeria. Frequent flooding associated with huge losses of lives and property in the study areas, as in most of urban Nigeria, persuade us to recommend that strategically placed civil society be supported by donor/funding organizations to promote integrated non-structural and traditional-structural measures to achieve urban flood resilience nationwide.

Flood resilience and sustainable development in urban Nigeria: integrating traditional and non-structural methods of mitigating and adapting to flooding in cross river state, south-eastern Nigeria. We examined application of non-structural measures in addition to conventional structural approaches by Government Agency and community for flood management in Cross River State (Nigeria) at: regional-ambit and community levels. We used focus group discussion in depth interview, and observation methods to collect datafrom primary and secondary sources. Our findings include: emphasis on structural flood control measures by government agencies contrasted to use of rudimentary non-structural approaches by communities. Conceptual frames proposed for managing disasters include: emphasizing future climate change impacts based on multiple scales (temporal, spatial and societal) and emphasizing historical response to disasters without increasing the visibility of climate change. We conclude that community institutions, non-government/civil society organizations should lead public institutions in promoting flood resilience based on integrated non-structural to structural measures and show recent developments regarding civil society coalition committed towards promoting environmental governance in Nigeria. Frequent flooding associated with huge losses of lives and property in the studyareas, as in most of urban Nigeria, persuade us to recommend that strategically placed civil society be supported by donor/funding organizations to promote integrated non-structural and traditional-structural measures to achieve urban flood resilience nationwide.

As the desire for high performance buildings increases, it is increasingly evident that engineers require reliable methods for the estimation of seismic demands on both structural and non-structural components. To this extent, improved tools for the prediction of floor spectra would assist in the assessment of acceleration sensitive non-structural and secondary components. Recently, a new procedure was successfully developed and tested for the simplified construction of floor spectra, at various levels of elastic damping, atop single-degree-of-freedom structures. This paper extends the methodology to multi-degree-of-freedom (MDOF) supporting systems responding in the elastic range, proposing a simplified modal combination approach for floor spectra over upper storeys and accounting for the limited filtering of the ground motion input that occurs over lower storeys. The procedure is tested numerically by comparing predictions with floor spectra obtained from time-history analyses of RC wall structures of 2- to 20-storeys in height. Results demonstrate that the method performs well for MDOF systems responding in the elastic range. Future research should further develop the approach to permit the prediction of floor spectra in MDOF systems that respond in the inelastic range. [ABSTRACT FROM AUTHOR]

Despite the seismic vulnerability of non-structural Exterior Infill Walls (EIWs), their resilient design has received minimal attention. This study addresses the issue by proposing a novel framework for predicting possible damage states of EIWs. The framework benefits from an automated combination of Building Information Modeling as a visualized 3D database of the building's components and the Machine Learning classification as the prediction engine. The framework's applicability is studied in a Proof of Concept example of the exterior walls of the buildings damaged in the 2017 earthquake in Kermanshah, Iran. The Extremely Randomized Trees classifier produced the best results for predicting new cases with an overall accuracy of %86. The trained model is used to develop a system for predicting the damage states of EIWs of a new building. The proposed framework works as a complementary tool in buildings' design and operation phases to enhance EIWs' seismic resilience. • Proposed a novel damage prediction framework for non-structural building systems. • Combined Building Information Modeling and Machine Learning Classification. • Applied the framework to the exterior infill walls in Kermanshah earthquake example. • Identified seismic stability features of exterior infill walls from the literature. • Framework represented %86 accuracy using the Extremely Randomized Trees classifier. [ABSTRACT FROM AUTHOR]

Background Bluetongue virus (BTV) is an arbovirus that is responsible for 'bluetongue', an economically important disease of livestock. Although BTV is well characterised at the protein level, less is known regarding its interaction with host cells. During studies of virus inclusion body formation we observed what appeared to be a large proportion of cells in mitosis. Although the modulation of the cell cycle is well established for many viruses, this was a novel observation for BTV. We therefore undertook a study to reveal in more depth the impact of BTV upon cell division. Methods We used a confocal microscopy approach to investigate the localisation of BTV proteins in a cellular context with their respective position relative to cellular proteins. In addition, to quantitatively assess the frequency of aberrant mitosis induction by the viral non-structural protein (NS) 2 we utilised live cell imaging to monitor HeLa-mCherry tubulin cells transfected with a plasmid expressing NS2. Results Our data showed that these 'aberrant mitoses' can be induced in multiple cell types and by different strains of BTV. Further study confirmed multiplication of the centrosomes, each resulting in a separate mitotic spindle during mitosis. Interestingly, the BTV NS1 protein was strongly localised to the centrosomal regions. In a separate, yet related observation, the BTV NS2 protein was co-localised with the condensed chromosomes to a region suggestive of the kinetochore. Live cell imaging revealed that expression of an EGFP-NS2 fusion protein in HeLa-mCherry tubulin cells also results in mitotic defects. Conclusions We hypothesise that NS2 is a microtubule cargo protein that may inadvertently disrupt the interaction of microtubule tips with the kinetochores during mitosis. Furthermore, the BTV NS1 protein was distinctly localised to a region encompassing the centrosome and may therefore be, at least in part, responsible for the disruption of the centrosome as observed in BTV infected mammalian cells. [ABSTRACT FROM AUTHOR]

We examined application of non-structural measures in addition to conventional structural approaches by Government Agency and community for flood management in Cross River State (Nigeria) at: regional-ambit and community levels. We used focus group discussion in depth interview, and observation methods to collect data from primary and secondary sources. Our findings include: emphasis on structural flood control measures by government agencies contrasted to use of rudimentary non-structural approaches by communities. Conceptual frames proposed for managing disasters include: emphasizing future climate change impacts based on multiple scales (temporal, spatial and societal) and emphasizing historical response to disasters without increasing the visibility of climate change. We conclude that community institutions, non-government/civil society organizations should lead public institutions in promoting flood resilience based on integrated non-structural to structural measures and show recent developments regarding civil society coalition committed towards promoting environmental governance in Nigeria. Frequent flooding associated with huge losses of lives and property in the study areas, as in most of urban Nigeria, persuade us to recommend that strategically placed civil society be supported by donor/funding organizations to promote integrated non-structural and traditional-structural measures to achieve urban flood resilience nationwide. [ABSTRACT FROM AUTHOR]

Abstract: India faced an epizootic of equine influenza in 2008–2009. The isolated viruses were typed as H3N8 and grouped with the clade 2 viruses of Florida sublineage on the basis of haemagglutinin (HA) gene sequence analysis. This report describes the genetic analysis and selection pressure of matrix (M) and non-structural 1 (NS1) genes of the Indian isolates. All isolates shared 98.41% and 99.54% homology with other clade 2 viruses of Asian origin for M1 and M2 amino acid (aa) sequences, respectively. There were 3 and 4 unique aa residue changes respectively in M1 and M2 proteins in all Asian isolates. Phylogenetic analysis revealed clustering of Indian and Chinese isolates in a separate group designated here as Asian clade for M gene. Indian and Chinese isolates shared homology ranging from 98.17% to 99.08% at aa level. The M and NS1 genes were under negative selection pressure with estimated magnitude of pressure (ω) 0.054, 0.581 and 0.30 for M1, M2 and NS1, respectively. [Copyright &y& Elsevier]

Background/Aims: The reason why patients with hepatitis C virus (HCV) genotype non-1 infection respond better to antiviral therapy than patients with genotype 1 infection is not known. The aim of this study is to explore the relation between the viral genotype, viral load, and the endogenous T cell response.Methods: The viral genotype, the viral load, and the endogenous proliferative T cell response to the non-structural 3 protein (NS3) was analysed using serum and peripheral blood mononuclear cells from 103 patients with chronic HCV infection.Results: Among 71 nontreated patients a T cell response was more common among those infected by genotype 3, as compared to those infected with genotype 1 (P<0.05). Among 32 patients undergoing antiviral therapy, presence of a T cell response was more common in genotype non-1 infected patients than in those infected by genotype 1 (P<0.01). Presence of a T cell response was related to a more rapid viral clearance (P<0,05), a negative HCV RNA test at week 12 (P<0.05), and a shorter viral half-life (P<0.05).Conclusions: The presence of an NS3-specific T cell response is related to the viral genotype and to a more rapid clearance of HCV RNA during antiviral therapy. [Copyright &y& Elsevier]

Recent natural hazards have exposed the dire consequence of damage and impact upon the built environment. It appears that one of the biggest challenges to the natural hazard mitigation community is how to improve the performance of older building and infrastructure to enhance their ability to withstand natural hazards. By improving their performance, the risk associated with buildings and infrastructure against natural hazards can be mitigated. Within the context of risk management of buildings against earthquakes, the general practice is to follow a three-step process, namely screening, evaluation and mitigation. Screening constitutes a preliminary evaluation process and sets priority for detailed evaluation. Evaluation compares a built environment with code requirements for new construction and sets priority for mitigation. Mitigation can be achieved by means of retrofit or replacement. Retrofit is intended to improve the performance of built environment as required. Replacement may be the only viable solution when economical, technical and environmental considerations are account for. [ABSTRACT FROM AUTHOR]

The 3′-ends of the genomes (9538 bp) of two wild-type respiratory bovine coronavirus (RBCV) isolates LSU and OK were obtained by cDNA sequencing. In addition, the 3′-end of the genome (9545) of the wild-type enteric bovine coronavirus (EBCV) strain LY-138 was assembled from available sequences and by cDNA sequencing of unknown genomic regions. Comparative analyses of RBCV and EBCV nucleotide and deduced amino acid sequences revealed that RBCV-specific nucleotide and amino acid differences were disproportionally concentrated within the S gene and the genomic region between the S and E genes. Comparisons among virulent and avirulent BCV strains revealed that virulence-specific nucleotide and amino acid changes were located within the S and E genes, and the 32 kDa open reading frame. [ABSTRACT FROM AUTHOR]

Flaviviruses related to hepatitis C virus (HCV) in suitable animal models may provide further insight into the role that cellular immunity contributes to spontaneous clearance of HCV. We characterised changes in lymphocyte populations in tamarins with an acute GBV-B infection, a hepatitis virus of the flaviviridae. Major immune cell populations were monitored in peripheral and intra-hepatic lymphocytes at high viraemia or following a period when peripheral virus was no longer detected. Limited changes in major lymphocyte populations were apparent during high viraemia; however, the proportions of CD3(+) lymphocytes decreased and CD20(+) lymphocytes increased once peripheral viraemia became undetectable. Intrahepatic lymphocyte populations increased at both time points post-infection. Distinct expression patterns of PD-1, a marker of T-cell activation, were observed on peripheral and hepatic lymphocytes; notably there was elevated PD-1 expression on hepatic CD4(+) T-cells during high viraemia, suggesting an activated phenotype, which decreased following clearance of peripheral viraemia. At times when peripheral vRNA was not detected, suggesting viral clearance, we were able to readily detect GBV-B RNA in the liver, indicative of long-term virus replication. This study is the first description of changes in lymphocyte populations during GBV-B infection of tamarins and provides a foundation for more detailed investigations of the responses that contribute to the control of GBV-B infection., (Crown Copyright © 2013. Published by Elsevier B.V. All rights reserved.)

Secreted infectious particles generated by the genotype 2a JFH-1 hepatitis C virus infectious clone are resistant to acidic pH, whereas intracellular virions remain acid-labile. Thus, JFH-1 particles are thought to undergo pH maturation as they are secreted from the cell. Here, we demonstrate that both infectious intracellular and secreted genotype 1a (H77)/JFH-1 chimaeric particles display enhanced acid sensitivity compared with JFH-1, although pH maturation still occurs upon release. Introduction of p7 sequences from genotype 1a infected HCV patients into the H77/JFH-1 background yielded variable effects on infectious particle production and sensitivity to small molecule inhibitors. However, two selected patient p7 sequences increased the acid stability of secreted, but not intracellular H77/JFH-1 particles, suggesting that p7 directly influences particle pH maturation via an as yet undefined mechanism. We propose that HCV particles vary in acid stability, and that this may be dictated by variations in both canonical structural proteins and p7., (© 2013 Elsevier Inc. All rights reserved.)

Infection with genotype 3 hepatitis C virus (HCV) is common throughout the world, however no direct-acting antiviral (DAA) has been approved to treat this genotype. We therefore attempted to develop novel genotype 3 replicons to facilitate the discovery and development of new HCV therapies. A novel Huh-7-derived cell line 1C but not Lunet cells enabled the selection of a few stable colonies of a genotype 3a subgenomic replicon (strain S52). Genotypic analysis revealed a mutation of P89L in the viral NS3 protease domain, which was confirmed to enhance genotype 3a RNA replication and enable the establishment of highly replicating luciferase-encoding replicons. Secondary adaptive mutations that further enhanced RNA replication were identified in the viral NS3 and NS4A proteins. In addition, cell lines that were cured of genotype 3a replicons demonstrated higher permissiveness specifically to genotype 3a HCV replication. These novel replicons and cell lines were then used to study the activity of approved and experimental HCV inhibitors. NS3 protease and non-nucleoside NS5B polymerase inhibitors often demonstrated substantially less antiviral activity against genotype 3a compared to genotype 1b. In contrast, nucleoside analog NS5B inhibitors and host-targeting HCV inhibitors showed comparable antiviral activity between genotypes 3a and 1b. Overall, the establishment of this novel genotype 3a replicon system, in conjunction with those derived from other genotypes, will aid the development of treatment regimens for all genotypes of HCV., (Copyright © 2013 Elsevier B.V. All rights reserved.)

Dengue virus (DENV) protein NS5 carries two mRNA cap methyltransferase (MTase) activities involved in the synthesis of a cap structure, (7Me)GpppA(2'OMe)-RNA, at the 5'-end of the viral mRNA. The methylation of the cap guanine at its N7-position (N7-MTase, (7Me)GpppA-RNA) is essential for viral replication. The development of high throughput methods to identify specific inhibitors of N7-MTase is hampered by technical limitations in the large scale synthesis of long capped RNAs. In this work, we describe an efficient method to generate such capped RNA, GpppA(2'OMe)-RNA₇₄, by ligation of two RNA fragments. Then, we use GpppA(2'OMe)-RNA₇₄ as a substrate to assess DENV N7-MTase activity and to develop a robust and specific activity assay. We applied the same ligation procedure to generate (7Me)GpppA-RNA₇₄ in order to characterize the DENV 2'-O-MTase activity specifically on long capped RNA. We next compared the N7- and 2'-O-MTase inhibition effect of 18 molecules, previously proposed to affect MTase activities. These experiments allow the validation of a rapid and sensitive method easily adaptable for high-throughput inhibitor screening in anti-flaviviral drug development., (Copyright © 2013 Elsevier B.V. All rights reserved.)